ATE512230T1 - Proteinherstellungsverfahren unter nutzung von yebf - Google Patents
Proteinherstellungsverfahren unter nutzung von yebfInfo
- Publication number
- ATE512230T1 ATE512230T1 AT05774845T AT05774845T ATE512230T1 AT E512230 T1 ATE512230 T1 AT E512230T1 AT 05774845 T AT05774845 T AT 05774845T AT 05774845 T AT05774845 T AT 05774845T AT E512230 T1 ATE512230 T1 AT E512230T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- fusion protein
- yebf
- polypeptide
- further comprise
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title 1
- 230000014616 translation Effects 0.000 title 1
- 108020001507 fusion proteins Proteins 0.000 abstract 6
- 102000037865 fusion proteins Human genes 0.000 abstract 6
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000007026 protein scission Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52212504P | 2004-08-18 | 2004-08-18 | |
| PCT/CA2005/001249 WO2006017929A1 (en) | 2004-08-18 | 2005-08-12 | Protein production method utilizing yebf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE512230T1 true ATE512230T1 (de) | 2011-06-15 |
Family
ID=35907194
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05774845T ATE512230T1 (de) | 2004-08-18 | 2005-08-12 | Proteinherstellungsverfahren unter nutzung von yebf |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20060246539A1 (enExample) |
| EP (1) | EP1791961B1 (enExample) |
| JP (1) | JP2008509682A (enExample) |
| AT (1) | ATE512230T1 (enExample) |
| AU (1) | AU2005274628A1 (enExample) |
| CA (1) | CA2576083A1 (enExample) |
| WO (1) | WO2006017929A1 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102006050332A1 (de) | 2006-10-25 | 2008-04-30 | Wacker Chemie Ag | DNS-Konstrukt und Verfahren zur fermentativen Herstellung von Fusionsproteinen |
| AU2012253359B2 (en) * | 2011-05-11 | 2017-06-08 | Children's Medical Center Corporation | Multiple antigen presenting immunogenic composition, and methods and uses thereof |
| CA2900008C (en) | 2013-02-07 | 2025-10-07 | Children's Medical Center Corporation | PROTEIN ANTIGENS THAT CONFER PROTECTION AGAINST COLONIZATION AND/OR PNEUMOCOCCAL DISEASE |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| US10435697B2 (en) * | 2014-11-03 | 2019-10-08 | Nanyang Technological University | Recombinant expression system that senses pathogenic microorganisms |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11365225B2 (en) | 2015-11-19 | 2022-06-21 | President And Fellows Of Harvard College | Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria |
| US12351805B2 (en) | 2015-11-19 | 2025-07-08 | Northeastern University | Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| EP3600400A4 (en) | 2017-03-28 | 2021-01-13 | The Children's Medical Center Corporation | MULTIPLE ANTIGEN PRESENTATION SYSTEM (MAPS) BASED STAPHYLOCOCCUS AUREUS VACCINE, IMMUNOGEN COMPOSITION AND THEIR USES |
| CN111065417B (zh) | 2017-06-23 | 2024-04-19 | 美商艾芬尼维克斯公司 | 免疫原性组合物 |
| TW202019470A (zh) | 2018-09-12 | 2020-06-01 | 美商艾芬尼維克斯公司 | 多價肺炎球菌疫苗 |
| KR102922338B1 (ko) | 2018-09-12 | 2026-02-05 | 더 칠드런스 메디칼 센터 코포레이션 | 폐렴구균 융합 단백질 백신 |
| CN113383080B (zh) * | 2019-02-15 | 2024-05-14 | 昂科斯密斯生物技术私人有限公司 | 用于增加蛋白质分泌的细菌表达载体 |
| JP7487951B2 (ja) * | 2019-02-24 | 2024-05-21 | オンコシミス バイオテック プライベート リミテッド | 細菌による組換えｇｌｐ－１ペプチドの連続生産方法 |
| US11471497B1 (en) | 2019-03-13 | 2022-10-18 | David Gordon Bermudes | Copper chelation therapeutics |
| US10973908B1 (en) | 2020-05-14 | 2021-04-13 | David Gordon Bermudes | Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine |
| US12537071B1 (en) | 2020-07-22 | 2026-01-27 | David Gordon Bermudes | Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins |
| WO2023039223A1 (en) | 2021-09-09 | 2023-03-16 | Affinivax, Inc. | Multivalent pneumococcal vaccines |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1335357C (en) * | 1987-10-30 | 1995-04-25 | Timothy M. Rose | Expression systems for preparation of polypeptides in prokaryotic cells |
| US5646016A (en) * | 1991-02-06 | 1997-07-08 | Genetics Institute, Inc. | Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules |
| US6033877A (en) * | 1995-11-02 | 2000-03-07 | Research Foundation Of State University Of New York | Peptide expression and delivery system |
| US6022952A (en) * | 1998-04-01 | 2000-02-08 | University Of Alberta | Compositions and methods for protein secretion |
| US6733997B1 (en) * | 1998-10-30 | 2004-05-11 | National University Of Singapore | Isolated nucleic acids encoding a secretory signal for expression and secretion of heterologous recombinant proteins |
| FI109361B (fi) | 1999-05-04 | 2002-07-15 | Korpela Timo | Mikrobinen proteiinin ilmentämisjärjestelmä |
| AU2001261242B2 (en) * | 2000-05-03 | 2006-12-14 | Expressive Constructs, Inc. | A method for improving recombinant protein stability and solubility |
| EP1343870A4 (en) * | 2000-11-09 | 2005-07-13 | Univ Queensland | BACTERIAL EXPRESSION |
| US20040142429A1 (en) * | 2001-01-25 | 2004-07-22 | Grant Chris W.M. | Method for the expression, purification, and structure recovery and handling of hydrophobic proteins, polypeptides or peptides |
| JP2004532033A (ja) * | 2001-05-04 | 2004-10-21 | コーネル リサーチ ファンデイション インコーポレイテッド | 発現し難いタンパク質用の迅速に開裂可能なｓｕｍｏ融合タンパク質発現系 |
| KR100447530B1 (ko) * | 2001-08-14 | 2004-09-08 | 한국과학기술원 | ＯｍｐＦ를 이용하여 목적 단백질을 대장균 세포외로분비생산하는 방법 |
| US6790051B1 (en) * | 2003-12-20 | 2004-09-14 | Lear Corporation | Connector for printed circuit board |
-
2005
- 2005-08-12 CA CA002576083A patent/CA2576083A1/en not_active Abandoned
- 2005-08-12 JP JP2007526148A patent/JP2008509682A/ja active Pending
- 2005-08-12 WO PCT/CA2005/001249 patent/WO2006017929A1/en not_active Ceased
- 2005-08-12 AU AU2005274628A patent/AU2005274628A1/en not_active Abandoned
- 2005-08-12 AT AT05774845T patent/ATE512230T1/de not_active IP Right Cessation
- 2005-08-12 EP EP05774845A patent/EP1791961B1/en not_active Expired - Lifetime
- 2005-08-15 US US11/203,168 patent/US20060246539A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2576083A1 (en) | 2006-02-23 |
| EP1791961B1 (en) | 2011-06-08 |
| US20060246539A1 (en) | 2006-11-02 |
| JP2008509682A (ja) | 2008-04-03 |
| EP1791961A4 (en) | 2008-11-19 |
| EP1791961A1 (en) | 2007-06-06 |
| AU2005274628A1 (en) | 2006-02-23 |
| WO2006017929A1 (en) | 2006-02-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |