JP2007534308A5 - - Google Patents
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- JP2007534308A5 JP2007534308A5 JP2006541541A JP2006541541A JP2007534308A5 JP 2007534308 A5 JP2007534308 A5 JP 2007534308A5 JP 2006541541 A JP2006541541 A JP 2006541541A JP 2006541541 A JP2006541541 A JP 2006541541A JP 2007534308 A5 JP2007534308 A5 JP 2007534308A5
- Authority
- JP
- Japan
- Prior art keywords
- photometrically
- labeled
- oligonucleotide
- labeling molecules
- labeling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000002372 labelling Methods 0.000 claims 6
- 108091034117 Oligonucleotide Proteins 0.000 claims 5
- 230000005284 excitation Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
Description
Claims (1)
- 標識オリゴヌクレオチドであって、以下:
オリゴヌクレオチド配列;および
少なくとも2つの光度測定可能なラベリング分子であって、当該少なくとも2つの光度測定可能なラベリング分子は、互いの励起波長の15nm以内にある励起波長を有し、ここで、少なくとも2つの光度測定可能なラベリング分子は当該オリゴヌクレオチドに連結され、そして当該オリゴヌクレオチドに連結された少なくとも2つの光度測定可能なラベリング分子は互いに3〜60ヌクレオチドの範囲内にある、
を含んでなり、
ここで、前記の少なくとも2つの光度測定可能なラベリング分子の少なくとも2つが互いから引き離される時に、前記の光度測定可能なラベリング分子の少なくとも2つがシグナルの検出可能な変化を発生する、
前記標識オリゴヌクレオチド。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52326303P | 2003-11-19 | 2003-11-19 | |
PCT/US2004/038946 WO2005051967A2 (en) | 2003-11-19 | 2004-11-19 | Oligonucleotides labeled with a plurality of fluorophores |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011177659A Division JP5307206B2 (ja) | 2003-11-19 | 2011-08-15 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007534308A JP2007534308A (ja) | 2007-11-29 |
JP2007534308A5 true JP2007534308A5 (ja) | 2011-10-06 |
Family
ID=34632765
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006541541A Withdrawn JP2007534308A (ja) | 2003-11-19 | 2004-11-19 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
JP2011177659A Expired - Fee Related JP5307206B2 (ja) | 2003-11-19 | 2011-08-15 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011177659A Expired - Fee Related JP5307206B2 (ja) | 2003-11-19 | 2011-08-15 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
Country Status (5)
Country | Link |
---|---|
US (2) | US7667024B2 (ja) |
EP (1) | EP1689764B1 (ja) |
JP (2) | JP2007534308A (ja) |
CN (1) | CN101076537B (ja) |
WO (1) | WO2005051967A2 (ja) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006002167A2 (en) * | 2004-06-17 | 2006-01-05 | University Of Florida Research Foudation, Inc. | Multi-acceptor molecular probes and applications thereof |
US9040305B2 (en) | 2004-09-28 | 2015-05-26 | Singulex, Inc. | Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry |
US8685711B2 (en) | 2004-09-28 | 2014-04-01 | Singulex, Inc. | Methods and compositions for highly sensitive detection of molecules |
US8092784B2 (en) | 2005-11-30 | 2012-01-10 | Biotium, Inc. | Enzyme substrate comprising a functional dye and associated technology and methods |
CA3086149A1 (en) * | 2006-04-04 | 2007-10-11 | Singulex, Inc. | Highly sensitive system and methods for analysis of troponin |
CN103543094B (zh) | 2007-12-19 | 2017-06-09 | 神谷来克斯公司 | 单分子检测用扫描分析器和使用方法 |
US10759824B2 (en) | 2008-09-03 | 2020-09-01 | Quantumdx Group Limited | Design, synthesis and use of synthetic nucleotides comprising charge mass tags |
WO2010026488A2 (en) | 2008-09-03 | 2010-03-11 | Quantumdx Group Limited | Methods and kits for nucleic acid sequencing |
WO2011068569A1 (en) | 2009-12-04 | 2011-06-09 | Biotium, Inc. | Heterocycle-substituted xanthene dyes |
CN101709335B (zh) * | 2009-12-10 | 2011-12-21 | 浙江大学 | 一种人乳头瘤病毒常见型四重荧光定量pcr分型检测方法 |
CN102762744B (zh) * | 2009-12-21 | 2017-07-25 | Seegene株式会社 | 利用靶信号产生引物进行的靶检测 |
WO2011078439A1 (en) * | 2009-12-24 | 2011-06-30 | Seegene, Inc. | Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals |
DE102010003781B4 (de) | 2010-04-08 | 2012-08-16 | Aj Innuscreen Gmbh | Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen |
EP2673381A4 (en) | 2011-02-07 | 2014-10-15 | Univ Toronto | BIOSONDES AND METHOD FOR THEIR USE |
JP6120449B2 (ja) * | 2011-05-06 | 2017-04-26 | キアゲン ゲーエムベーハー | リンカーを介して結合した標識を含むオリゴヌクレオチド |
US9056885B2 (en) | 2011-11-21 | 2015-06-16 | Promega Corporation | Carboxy X rhodamine analogs |
EP2809813B1 (en) | 2012-02-03 | 2018-08-08 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
WO2014022827A1 (en) | 2012-08-03 | 2014-02-06 | California Institute Of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
ES2653642T3 (es) * | 2012-12-27 | 2018-02-08 | Seegene, Inc. | Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO |
CA2897474A1 (en) | 2013-01-24 | 2014-07-31 | California Institute Of Technology | Chromophore-based characterization and detection methods |
WO2014144548A2 (en) | 2013-03-15 | 2014-09-18 | Nanobiosym, Inc. | Systems and methods for mobile device analysis of nucleic acids and proteins |
JP6190534B2 (ja) | 2013-08-22 | 2017-08-30 | ソニー株式会社 | 水溶性蛍光染料又は有色染料及びその使用方法 |
US10227638B2 (en) | 2014-06-02 | 2019-03-12 | Base4 Innovation Ltd. | Nucleotide polymorphism detection method |
WO2016081549A1 (en) * | 2014-11-20 | 2016-05-26 | Ampliwise Inc. | Compositions, methods, systems and kits for nucleic acid amplification and analysis |
WO2016138457A1 (en) | 2015-02-26 | 2016-09-01 | Sony Corporation | Phenylethynylnaphthalene dyes and methods for their use |
US11827661B2 (en) | 2015-02-26 | 2023-11-28 | Sony Group Corporation | Water soluble fluorescent or colored dyes comprising conjugating groups |
JP2019503174A (ja) | 2016-01-04 | 2019-02-07 | クァンタムディーエックス グループ リミテッドQuantumdx Group Limited | 電荷量タグを含む合成ヌクレオチドのデザイン、合成、および使用 |
JP7145079B2 (ja) | 2016-04-01 | 2022-09-30 | ソニーグループ株式会社 | 剛性のスペーシング基を有する超明色ダイマーまたはポリマー染料 |
BR112018070164B1 (pt) | 2016-04-01 | 2022-09-27 | Sony Corporation Of America | Corantes diméricos ou poliméricos ultrabrilhantes |
US9851359B2 (en) | 2016-04-06 | 2017-12-26 | Sony Corporation Of America | Ultra bright dimeric or polymeric dyes with spacing linker groups |
EP3455238A1 (en) | 2016-05-10 | 2019-03-20 | Sony Corporation | Ultra bright polymeric dyes with peptide backbones |
EP3455299B1 (en) | 2016-05-10 | 2024-01-17 | Sony Group Corporation | Compositions comprising a polymeric dye and a cyclodextrin and uses thereof |
WO2017197144A1 (en) | 2016-05-11 | 2017-11-16 | Sony Corporation | Ultra bright dimeric or polymeric dyes |
US11377563B2 (en) | 2016-06-06 | 2022-07-05 | Sony Corporation | Ionic polymers comprising fluorescent or colored reporter groups |
WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
EP3491071A1 (en) | 2016-07-29 | 2019-06-05 | Sony Corporation | Ultra bright dimeric or polymeric dyes and methods for preparation of the same |
CN111315415A (zh) | 2017-10-05 | 2020-06-19 | 索尼公司 | 可编程的聚合药物 |
JP2021503450A (ja) | 2017-11-16 | 2021-02-12 | ソニー株式会社 | プログラム可能なポリマー薬 |
EP4137818A1 (en) | 2018-03-19 | 2023-02-22 | Sony Group Corporation | Use of divalent metals for enhancement of fluorescent signals |
WO2019182766A1 (en) | 2018-03-21 | 2019-09-26 | Sony Corporation | Polymeric tandem dyes with linker groups |
JP2021529194A (ja) | 2018-06-27 | 2021-10-28 | ソニーグループ株式会社 | デオキシリボースを含むリンカー基を有するポリマー色素 |
CA3127102A1 (en) * | 2019-01-23 | 2020-07-30 | Quantum-Si Incorporated | High intensity labeled reactant compositions and methods for sequencing |
WO2021004379A1 (zh) * | 2019-07-05 | 2021-01-14 | 南京金斯瑞生物科技有限公司 | 一种荧光标记核酸及其合成方法 |
WO2021062176A2 (en) | 2019-09-26 | 2021-04-01 | Sony Corporation | Polymeric tandem dyes with linker groups |
EP4038081A1 (en) * | 2019-09-30 | 2022-08-10 | Sony Group Corporation | Nucleotide probes |
US20230279479A1 (en) * | 2021-12-29 | 2023-09-07 | Co-Diagnostics, Inc. | Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores |
US20230366038A1 (en) | 2022-05-16 | 2023-11-16 | Bioo Scientific Corporation | Compositions and methods relating to loop mediated isothermal amplification (lamp) |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5654419A (en) * | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
WO1997027317A1 (en) * | 1996-01-23 | 1997-07-31 | Affymetrix, Inc. | Nucleic acid analysis techniques |
US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
US6312894B1 (en) * | 1995-04-03 | 2001-11-06 | Epoch Pharmaceuticals, Inc. | Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders |
DE69610304T2 (de) * | 1995-05-05 | 2001-04-05 | Perkin Elmer Corp | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
US6008373A (en) * | 1995-06-07 | 1999-12-28 | Carnegie Mellon University | Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer |
DE69738687D1 (de) * | 1996-04-12 | 2008-06-26 | Phri Properties Inc | Sonden, kits und assays |
US5800996A (en) * | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
ATE295427T1 (de) * | 1996-06-04 | 2005-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5696157A (en) * | 1996-11-15 | 1997-12-09 | Molecular Probes, Inc. | Sulfonated derivatives of 7-aminocoumarin |
US6037137A (en) * | 1997-02-20 | 2000-03-14 | Oncoimmunin, Inc. | Fluorogenic peptides for the detection of protease activity |
US6893868B2 (en) * | 1997-02-20 | 2005-05-17 | Onco Immunin, Inc. | Homo-doubly labeled compositions for the detection of enzyme activity in biological samples |
US5846726A (en) * | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
US6130101A (en) * | 1997-09-23 | 2000-10-10 | Molecular Probes, Inc. | Sulfonated xanthene derivatives |
US6485901B1 (en) * | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
US6133445A (en) * | 1997-12-17 | 2000-10-17 | Carnegie Mellon University | Rigidized trimethine cyanine dyes |
ATE414169T1 (de) * | 1998-03-05 | 2008-11-15 | Johnson & Johnson Res Pty Ltd | Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits |
GB2341189B (en) | 1998-08-31 | 2001-06-13 | Amersham Pharm Biotech Inc | Energy transfer dyes |
US6573047B1 (en) * | 1999-04-13 | 2003-06-03 | Dna Sciences, Inc. | Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation |
US6472153B1 (en) * | 1999-10-26 | 2002-10-29 | Epoch Biosciences, Inc. | Hybridization-triggered fluorescent detection of nucleic acids |
GB2359809B (en) * | 1999-12-24 | 2004-01-14 | Sumitomo Chemical Co | Phenylacetylene compound liquid crystal composition, polymer optically anisotropic product, and liquid crystal or optical element |
CA2417986C (en) * | 2000-08-11 | 2013-11-26 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
AU2001294859A1 (en) * | 2000-09-29 | 2002-04-08 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
-
2004
- 2004-11-19 CN CN200480040647.1A patent/CN101076537B/zh not_active Expired - Fee Related
- 2004-11-19 US US10/993,625 patent/US7667024B2/en not_active Expired - Fee Related
- 2004-11-19 WO PCT/US2004/038946 patent/WO2005051967A2/en active Application Filing
- 2004-11-19 EP EP04811635A patent/EP1689764B1/en not_active Not-in-force
- 2004-11-19 JP JP2006541541A patent/JP2007534308A/ja not_active Withdrawn
-
2009
- 2009-12-23 US US12/646,514 patent/US20100240103A1/en not_active Abandoned
-
2011
- 2011-08-15 JP JP2011177659A patent/JP5307206B2/ja not_active Expired - Fee Related
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