JP2006219407A - Hair papilla cell proliferation promoter and hair tonic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a hair papilla cell proliferation promoter containing a natural extract, and to provide a hair tonic containing the hair papilla cell proliferation promoter. <P>SOLUTION: This hair papilla cell proliferation promoter is characterized by containing one or more plant extracts selected from the group consisting of a Coix lachrymal-jobi L. extract, a Thymus serpyllum L. extract, an Equisetum arvense L. extract, an Acorus calamus L. extract, a Rosmarinus officinalis L. extract, a Curcuma longa L. extract, a Betula alba L. extract and a Camellia sinensis extract, as an active ingredient. The hair tonic is characterized by containing the hair papilla cell proliferation promoter. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a hair papilla cell growth promoter and a hair restorer.

  Hair repeats growth and loss according to a periodic hair cycle (hair cycle) consisting of a growth period, a regression period, and a rest period. Of these hair cycles, the stage at which new hair follicles are formed from the resting stage to the growing stage is considered to be the most important for hair growth, and the proliferation and differentiation of hair follicle epithelial cells at this stage It is believed that the dermal papilla cells play an important role. The dermal papilla cell is a cell located inside the hair follicle epithelial cell composed of outer root sheath cells and matrix cells in the vicinity of the hair root, and located in the root portion of the hair root wrapped in the basement membrane. It plays an important role in the growth / differentiation of hair follicle epithelial cells and the formation of hair, such as by acting on epithelial cells to promote their proliferation (see Non-Patent Document 1).

  Thus, dermal papilla cells play the most important role in the proliferation and differentiation of hair follicle epithelial cells and the formation of hair. Conventionally, the target substance is brought into contact with cultured dermal papilla cells to proliferate the cells. There has been proposed a method for testing the hair-growth effect of the target substance by specifying the presence and / or absence and / or strength of the skin (see Patent Document 1).

In addition, conventionally, as herbal medicines having a hair papillary cell growth promoting action, for example, an ogi extract, an auren extract, an anemone extract, and the like have been proposed (see Patent Document 2 and Patent Document 3). As described above, the demands of customers for various natural preparations that are excellent in safety and productivity, can be ingested on a daily basis, and have an excellent hair papillary cell proliferation promoting action while being inexpensive are extremely strong. The current situation is that no satisfactory product has yet been provided.
JP-A-10-229978 JP-A-9-208431 Japanese Patent Laid-Open No. 11-12134 “Trends Genet”, 1992, Vol. 8, pp. 56-61

  The present invention finds a substance having a dermal papilla cell proliferation promoting action among highly safe natural products, a dermal papilla cell proliferation promoter containing the substance as an active ingredient, and a hair growth that contains the dermal papilla cell proliferation promoter The purpose is to provide an agent.

  In order to solve the above-mentioned problems, the hair papilla cell growth promoter of the present invention comprises a pearl barley extract, a wild thyme extract, a cedar extract, a shobu extract, a rosemary extract, a turmeric extract, a birch extract, and a kocha. One or two or more kinds of plant extracts selected from the group consisting of extracts are contained as active ingredients, and the hair restorer of the present invention is characterized in that the hair papilla cell proliferation promoter is blended. To do.

  According to the present invention, one or two kinds selected from the group consisting of pearl barley extract, wild thyme extract, cedar extract, ginger extract, rosemary extract, turmeric extract, birch extract and kocha extract It is possible to provide a hair papilla cell growth promoter that contains the above plant extract as an active ingredient and is excellent in safety, and a hair growth agent that contains the hair papilla cell growth promoter.

The present invention will be described below.
[Hair papillary cell growth promoter]
The hair papilla cell proliferation promoter of the present invention is selected from the group consisting of pearl barley extract, wild thyme extract, horsetail extract, shobu extract, rosemary extract, turmeric extract, birch extract and kocha extract. Contains one or more plant extracts as active ingredients.

  Here, in the present invention, as the “extract”, one or two or more kinds of plants selected from the group consisting of pearl barley, wild thyme, cedar, shobu, rosemary, turmeric, birch and kocha can be obtained as an extraction raw material. An extract, a diluted solution or a concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product or a purified product thereof are included.

  Extraction raw materials used in the present invention are pearl barley (scientific name: Coix lachrymal-jobi L.), wild time (scientific name: Thymus serpyllum L.), sugina (scientific name: Equisetum arvense L.), shobu (scientific name: Acorus calamus L.). ), Rosemary (scientific name: Rosmarinus officinalis L.), turmeric (scientific name: Curcuma longa L.), birch (scientific name: Betula alba L.) or kocha (plant name: cha, scientific name: Camellia sinensis). When using two or more kinds of plants as an extraction raw material, the above plants can be used in any combination.

  Barley (Coix lachrymal-jobi L.) is a plant native to the Indochina region of China, and is an annual plant belonging to the grass family cultivated in the warm southwestern part of Japan. can do. Examples of constituent parts that can be used as an extraction raw material include leaves, branches, bark parts, trunks, stem parts, fruit parts, seed parts, flower parts, root parts, or a mixture of these parts, but preferably Is the seed part. The seed part of pearl barley is particularly called Yokuinin, and is used for purposes such as wet water wetting, clean fever, drainage, numbness, and splenic pain.

  Wild thyme (Thymus serpyllum L.) is a perennial plant belonging to the family Lamiaceae distributed from Afghanistan to China, Himalayas, Japan, etc., and can be easily obtained from these regions. Examples of constituent parts that can be used as an extraction raw material include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts and the like, root parts, or a mixture of these parts. The above-mentioned part is preferable.

  Horsetail (Equisetum arvense L.) is a summer green perennial fern plant that belongs to the family Crestaceae widely distributed in the northern hemisphere and beyond, and can be easily obtained from these regions. Examples of constituent parts that can be used as an extraction raw material include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts and the like, root parts, or a mixture of these parts. Preferably, it is whole grass.

  Ginger (Acorus calamus L.) is a perennial plant belonging to the taro family distributed in East Asia, India, Japan, etc., and can be easily obtained from these regions. Examples of constituent parts that can be used as the raw material for extraction include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts and other above-ground parts, rhizome parts, and mixtures of these parts. Although it is mentioned, Preferably it is a rhizome part.

  Rosemary (Rosmarinus officinalis L.) is an evergreen shrub belonging to the family Lamiaceae distributed in Europe, Japan and the like, and can be easily obtained from these regions. Examples of constituent parts that can be used as an extraction raw material include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts and the like, root parts, or a mixture of these parts. Preferably, they are leaves and flowers.

  Turmeric (Curcuma longa L.) is a perennial plant belonging to the ginger family distributed in China, India, Indonesia, Taiwan, Japan, etc., and can be easily obtained from these regions. Examples of constituent parts that can be used as the raw material for extraction include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts and other above-ground parts, rhizome parts, and mixtures of these parts. Although it is mentioned, Preferably it is a rhizome part.

  Birch (Betula alba L.) is a deciduous tree plant belonging to the birch family distributed in Mongolia from China, and can be easily obtained from these regions. Examples of constituent parts that can be used as the raw material for extraction include leaf parts, branch parts, bark parts, trunk parts, stem parts, fruit parts, seed parts, flower parts and other above-ground parts, rhizome parts, and mixtures of these parts. Although it is mentioned, Preferably it is a bark part.

  Koucha means a fermented leaf and / or stem of tea (scientific name: Camellia sinensis). Cha is an evergreen small shrub belonging to the Camellia family distributed in China, India, Japan, etc., and can be easily obtained from these areas. The kocha as an extraction raw material of the hair papilla cell growth promoter of the present invention is preferably a fermented leaf and / or stem part of Assamcha (scientific name: Camellia sinensis var. Assamica Pierre).

  Details of substances with a papillary cell growth-promoting action contained in pearl barley extract, wild thyme extract, cedar extract, shobu extract, rosemary extract, turmeric extract, birch extract or kocha extract are unknown However, an extract having a hair papillary cell growth promoting action can be obtained from these plants by an extraction method generally used for the extraction of plants.

  For example, after drying the plant, it is pulverized as it is or using a crusher, and subjected to extraction with an extraction solvent, whereby an extract having a hair papillary cell proliferation promoting action can be obtained. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing pretreatment such as degreasing, extraction treatment with the polar solvent of the plant can be efficiently performed.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These may be used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. Is preferred.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; -C2-C5 polyhydric alcohols, such as butylene glycol, propylene glycol, and glycerol, etc. are mentioned.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a liquid mixture of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by weight of a lower aliphatic alcohol with respect to 10 parts by weight of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by mass of a lower aliphatic ketone with 10 parts by mass of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by mass of polyhydric alcohol with respect to parts by mass.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.

  Purification can be performed by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like. The obtained extract can be used as it is as an active ingredient of a dermal papilla cell growth promoter, but a concentrated solution or a dried product is easier to use.

  Since each of the above plant extracts has a characteristic odor, it is possible to perform purification for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in the physiological activity, but it is blended in a hair restorer. In some cases, it is not used in large quantities, so there is no practical problem even if it is not purified.

  The barley extract, wild thyme extract, cedar extract, ginger extract, rosemary extract, turmeric extract, birch extract or kocha extract obtained as described above has an effect of promoting the growth of dermal papilla cells. Therefore, it can be used as an active ingredient of a hair papilla cell proliferation promoter utilizing its action.

  The hair papilla cell proliferation promoter of the present invention is one or more selected from pearl barley extract, wild thyme extract, horsetail extract, shobu extract, rosemary extract, turmeric extract, birch extract and kocha extract. It may consist of only two or more plant extracts, or may be a formulation of the plant extract.

  The above plant extract should be formulated into an arbitrary dosage form such as powder, granule, liquid, etc. according to a conventional method using a pharmaceutically acceptable carrier such as dextrin, cyclodextrin and any other auxiliary agent. Can do. At this time, as an auxiliary agent, for example, an excipient, a stabilizer, a flavoring agent and the like can be used. The plant extract can be used by blending with other compositions (hair cosmetics, etc.), and can also be used as an ointment, an external liquid, a patch and the like.

  The dermal papilla cell proliferation promoter of the present invention can be used as an active ingredient by blending with other natural extracts having a dermal papilla cell proliferation promoting effect, if necessary.

  The hair papilla cell proliferation promoter of the present invention is a hair papilla cell growth possessed by pearl barley extract, wild thyme extract, horsetail extract, shobu extract, rosemary extract, turmeric extract, birch extract or kocha extract. Promotes dermal papilla cells through the promoting action, promotes the proliferation and differentiation of hair follicle epithelial cells and the formation of hair, and prevents the transition from the growth phase to the regression phase and the resting phase in the hair cycle , Can extend the growth period. As a result, the dermal papilla cell proliferation promoter of the present invention can promote hair growth and / or hair growth, and can prevent and improve various alopecia (eg, male pattern alopecia, alopecia areata). Can do. However, the dermal papilla cell proliferation promoter of the present invention can be used for all applications that are meaningful for exerting a dermal papilla cell proliferation promoting effect in addition to these applications.

[Hair restorer]
The hair growth agent of the present invention comprises the above-described hair papilla cell proliferation promoter (pearl barley extract, wild thyme extract, cedar extract, shobu extract, rosemary extract, turmeric extract, birch extract and kocha extract). Including only one or two or more plant extracts selected from the group).

  Pearl barley extract, wild thyme extract, cedar extract, shobu extract, rosemary extract, turmeric extract, birch extract, or kocha extract has an action to promote hair papillary cell proliferation and is applied to the scalp It is suitable for blending in a hair restorer as a skin external preparation because it is excellent in use feeling and safety. In this case, one or more plants selected from the group consisting of pearl barley extract, wild thyme extract, cedar extract, ginger extract, rosemary extract, turmeric extract, birch extract and kocha extract A hair papilla cell growth promoter formulated from the extract may be added, or pearl extract, wild thyme extract, cedar extract, shobu extract, rosemary extract, turmeric extract, birch extract or The kocha extract may be blended as it is. By blending the hair papilla cell growth promoter or the plant extract with a hair growth agent, the hair growth agent can be imparted with a hair papilla cell growth promotion effect. As a result, the hair restorer of the present invention can promote hair growth and / or hair growth, and can prevent and improve various alopecias (for example, male pattern alopecia, alopecia areata).

  The form of the hair restorer of the present invention is not particularly limited, and specific examples thereof include hair tonic, hair cream, hair liquid, shampoo, rinse, pomade and the like.

  The compounding amount of the active ingredient in the hair restorer can be appropriately adjusted according to the type of the hair restorer, but is preferably about 0.0001 to 10% by mass in terms of a plant extract, particularly about It is preferable that it is 0.001-1 mass%.

  The hair growth agent of the present invention, together with the above-mentioned dermal papilla cell proliferation promoter, is any physiologically active substance or auxiliary agent depending on the application, such as astringent, bactericidal, antibacterial agent, ultraviolet absorber, moisturizer, cell activator, anti-inflammatory agent・ Antiallergic agents, antioxidant / active oxygen scavengers, etc. can be used in combination. When used in combination, it may result in a more general product, and a synergistic effect with other active ingredients used in combination may produce an effect that is more than expected. .

  The hair papilla cell growth promoter or hair restorer of the present invention is preferably applied to humans, but may be applied to animals other than humans as long as the respective effects are exhibited. You can also.

  Hereinafter, although a manufacture example and a test example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Production Example 1] Production of each plant extract To 300 g of coarsely pulverized seeds of pearl barley was added 3000 mL of extraction solvent, heated and extracted at 80 ° C. for 2 hours with gentle stirring, and filtered while hot. The obtained filtrate was concentrated under reduced pressure at 40 ° C., and further dried with a vacuum dryer to obtain a pearl barley extract. Table 1 shows the yield of each extract when water, 50 mass% ethanol (mass ratio of water to ethanol of 1: 1), and ethanol are used as the extraction solvent.

  In the same manner as above, wild thyme leaf, whole horsetail grass, ginger rhizome, rosemary leaf, turmeric rhizome, birch bark or kocha (completely fermented assamcha leaf) As an extraction raw material, extracts from each plant were obtained. Table 1 shows the yield of each extract when water, 50 mass% ethanol, or ethanol was used as the extraction solvent.

[Test Example 1] Hair papilla cell proliferation promoting action test Each plant extract obtained in Production Example 1 was tested for hair papilla cell proliferation promoting action as follows.

Normal human hair hair papilla cells were cultured using a hair papilla cell growth medium (manufactured by Cell Application Inc.) containing 2% fetal bovine serum (FBS) and growth additives, and then cells were collected by trypsin treatment. The collected cells were diluted to a cell density of 1.0 × 10 ⁴ cells / mL using DMEM medium containing 10% FBS, then seeded at 200 μL per well in a collagen-coated 96-well plate and cultured for 3 days. . After culturing, the medium was removed, and 200 μL of a sample solution in which each plant extract was dissolved in serum-free DMEM was added to each well and further cultured for 4 days.

  The dermal papilla cell proliferation promoting effect was measured using MTT assay. After completion of the culture, the medium was removed, and 100 μL of MTT (final concentration 0.4 mg / mL) dissolved in serum-free DMEM was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. As a control, the same measurement was performed when serum-free DMEM was added instead of the sample solution. From the obtained results, the dermal papilla cell proliferation promotion rate (%) was calculated based on the following formula.

Growth rate of dermal papilla cells (%) = A / B × 100
In the formula, A represents “absorbance when a sample is added”, and B represents “absorbance when no sample is added”.
The results of the above test are shown in Table 2.

  As shown in Table 2, pearl extract, wild thyme extract, cedar extract, shobu extract, rosemary extract, turmeric extract, birch extract and kocha extract are excellent in promoting the growth of dermal papilla cells. It was confirmed to have

The hair papilla cell growth promoter and hair restorer of the present invention can greatly contribute to the prevention and improvement of various alopecia (eg, male pattern alopecia, alopecia areata) and the like.

Claims (2)

  One or more plant extracts selected from the group consisting of pearl barley extract, wild thyme extract, cedar extract, shobu extract, rosemary extract, turmeric extract, birch extract and kocha extract A hair papilla cell growth promoter characterized by containing as an active ingredient. A hair growth agent comprising the hair papilla cell growth promoter according to claim 1.