JP2006193510A - Active oxygen-eliminating agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To aim at the expansion of uses of Terminalia by finding out the new activities of the Terminalia. <P>SOLUTION: This active oxygen-eliminating agent consists of a processed material obtained from the Terminalia. Since the active oxygen-eliminating agent can hold a component easily degenerated by the active oxygen, it can be used also as a food additive. As the raw material of such the processed material, especially, the fruit section except for the seeds of the Terminalia can be used preferably. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an active oxygen scavenger or food additive comprising a processed product obtained from terminaria.

  Terminalia is a plant of the family Sequimidae. For example, in India, the fruit is mainly used as an antidiarrheal agent and the ripe fruit is used as an antidiarrheal agent.

Recently, it has been found that the fruit of Terminalia bellirica, a kind of terminaria, has an action to prevent vascular fibrosis, and prevents or prevents vascular fibrosis consisting of its dry powder or extract. A therapeutic agent is disclosed (Patent Document 1). However, the effects of terminaria have not yet been fully studied, and terminaria is not fully utilized at present.
JP 2004-75584 A

  An object of the present invention is to expand the usage of terminaria by finding a new action of terminaria.

  The present inventor has found that a workpiece obtained from terminaria has an active oxygen scavenging ability, and has completed the present invention.

  The present invention provides an active oxygen scavenger comprising a processed product obtained from terminaria.

  Furthermore, this invention provides the food additive which consists of a processed material obtained from terminaria.

  According to the present invention, a workpiece obtained from terminaria has excellent active oxygen scavenging ability and can be used as an active oxygen scavenger. Further, it can be used as a food additive for maintaining the quality of food.

(Processed products obtained from Terminaria)
As used herein, “terminaria” refers to a plant belonging to the genus Terminalia. Examples of plants belonging to the genus Terminalia include Terminalia bellirica (belerica), Terminalia catappa, Terminalia tomentosa, Terminalia citrina, Terminalia phellocarpa, Terminalia copelandii, Terminalia brassi, Terminalia ivorensis, Terminalia superba, Terminalia arjuna, and Terminalia chebula. Among these, Terminalia bellirica (belerica), Terminalia arjuna, and Terminalia chebula are preferred, and Terminalia bellirica (belerica) and Terminalia chebula are more preferred.

  In the present invention, a processed product obtained from terminaria (hereinafter sometimes simply referred to as processed product) is a dry process or a pulverization process on parts such as leaves, bark, roots, flowers, xylem, fruits and seeds of terminaria. , A thing obtained by giving a pressing process, an extraction process, etc. Examples of such processed products include dried products, pastes, juices and extracts. In the present invention, any part of Terminaria may be used, and particularly a part other than seeds is preferable, and a part (fruit skin or pulp part) excluding leaves, bark, and fruit seeds is more preferable.

  The dried product, which is one form of the processed product, can be obtained, for example, by subjecting each portion of the terminaria to sun drying, half sun drying, shade drying, heat drying, room temperature drying, freeze drying, and the like. After drying, if necessary, it may be pulverized using a ball mill, a hammer mill or the like to obtain a dry powder.

  The paste which is one form of the processed product can be obtained, for example, by pulverizing each part of the terminaria with a mascolloider or the like without drying. Further, the juice is obtained by filtering or centrifuging the paste. Squeezed juice can also be obtained by squeezing each part of the above terminaria. The obtained juice may be used as it is, or may be dried to obtain an extract powder.

  The extract which is one form of the processed product is obtained by, for example, extracting each part of the terminia as it is from a paste or a dried product using water or an organic solvent. In particular, it is preferable to extract from paste or dry powder.

  Examples of the solvent that can be used to obtain the extract include water, an organic solvent, and a mixed solution of water and an organic solvent. Examples of the organic solvent include methanol, ethanol, n-propanol, n-butanol, acetone, Hexane, cyclohexane, propylene glycol, ethyl methyl ketone, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene, etc. Can be mentioned. Of these, polar organic solvents are preferred, more preferably ethanol, n-butanol, methanol, acetone, propylene glycol, and ethyl acetate, most preferably ethanol.

  The amount of the solvent used for extraction is not particularly limited as long as the components contained in the terminaria are sufficiently extracted. For example, when obtaining an extract from the dried product of Terminaria, the amount of the solvent is preferably 1 to 50 parts by mass with respect to 1 part by mass of the dried product. When obtaining an extract from other than the dried product, the amount of the solvent is preferably 0.1 to 30 parts by mass with respect to 1 part by mass of the dried product.

  The extraction temperature is not particularly limited, and can be appropriately set according to the boiling point of the solvent used. In order to extract efficiently, extraction temperature becomes like this. Preferably it is 10 degreeC or more, More preferably, it is 20 degreeC or more, Preferably it is 120 degrees C or less, More preferably, it is below the boiling point of the solvent to be used.

  The extraction time can be appropriately set depending on the amount of extraction solvent used or the extraction temperature. The extraction time is preferably 30 minutes or longer, more preferably 1 hour or longer, preferably 72 hours or shorter, more preferably 48 hours or shorter.

  In this way, an extract can be obtained from the terminaria. The obtained extract may be used as an extract as it is, or may be concentrated by a method commonly used by those skilled in the art and used as an extract in the form of a paste or powder, if necessary. In order to concentrate the extract, heating to dryness, vacuum concentration to dryness, freeze drying, and the like are performed. Among these, from the viewpoint of suppressing the denaturation of the components in the extract, it is preferably vacuum-concentrated to dryness or freeze-dried.

  Furthermore, in order to make the obtained extract more active, this extract was synthesized with a synthetic adsorbent (Diaion HP20, Sephabies SP825, Amberlite XAD4, MCIgelCHP20P, etc.) and dextran resin (Sephadex LH-20, etc.). ) Can be used to fractionate or concentrate the components.

(Active oxygen scavenger)
The active oxygen scavenger of this invention consists of the processed material obtained from the above-mentioned terminaria. The active oxygen scavenger of the present invention exhibits the same activity as SOD present in the living body, and can quickly scavenge active oxygen.

  Active oxygen refers to oxygen and its related compounds activated more than normal oxygen, and in particular in relation to living organisms, superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like can be mentioned. It has been clarified that these active oxygens cause various tissue damages when they are excessively present in the living body.

  In vivo, superoxide, which is usually produced in vivo and serves as a starting material for other active oxygen, is sequentially eliminated by the catalytic action of superoxide dismutase (SOD) contained in cells. However, when the production of superoxide is excessive or when the action of SOD is reduced as in the body of an elderly person, the superoxide is not sufficiently eliminated and the superoxide concentration in the living body becomes high. As a result, tissue damage such as rheumatoid arthritis and Behcet's disease, cataracts, spots, freckles, wrinkles, stiff shoulders, coldness, etc. are caused. Therefore, it is important to quickly eliminate active oxygen in order to prevent various diseases in vivo.

  The intake amount of the active oxygen scavenger of the present invention is not particularly limited, and varies depending on the form of a processed product such as an extract or a dry powder. For example, in the case of an extract of terminaria, the daily intake amount of an adult is preferably 10 mg or more, more preferably 20 mg or more, preferably 1000 mg or less, more preferably 600 mg or less in terms of dry mass. In the case of a dry powder of terminaria, the daily intake of an adult is preferably 100 mg or more, more preferably 200 mg or more, preferably 10,000 mg or less, more preferably 6000 mg or less.

(Food additive)
Since the processed product obtained from Terminaria has excellent active oxygen scavenging ability, it can be used as a food additive for maintaining food quality.

  For example, unstable components such as ascorbic acid, thiamine derivatives, and polyphenols that are present in foods are denatured by active oxygen and cause inactivation, discoloration, and the like. Therefore, by adding the food additive of the present invention to food, it is possible to prevent these unstable components, particularly water-soluble components, from being denatured by active oxygen. Can prevent.

  The compounding quantity in the foodstuff of the food additive of this invention is not restrict | limited, It changes with aspects of processed materials, such as an extract and a dry powder. For example, in the case of an extract of terminaria, the blending amount in the food is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, preferably 90% by mass or less, in terms of dry mass. More preferably, it is 60 mass% or less. In the case of dry powder of terminaria, the blending amount in the food is preferably 0.001% by mass or more, more preferably 0.01% by mass or more, preferably 90% by mass or less, more preferably 60% by mass or less. It is. When using paste, squeezed juice, and extract powder, the dry mass may be equivalent to the dry powder of terminaria.

  The food additive of the present invention can be added to any form of food. Examples of such food forms include beverages, granules, tablets, gums, candies, troches, jelly, gummi, tea bags and the like.

  The active oxygen scavenger and food additive of the present invention are optionally mixed with an excipient, a bulking agent, a binder, a thickener, an emulsifier, a colorant, a fragrance, and a mineral (iron, magnesium, calcium, zinc, selenium). And other ingredients such as other food ingredients and pharmaceutical ingredients.

Examples of the food additive of the present invention include royal jelly, propolis, vitamins (A, B ₁ , B ₂ , B ₆ , B ₁₂ , niacin, C, D, E, K, folic acid, pantothenic acid, biotin, and the like. Derivatives), chitin, chitosan, lecithin, polyphenols (flavonoids, their derivatives, etc.), carotenoids (lycopene, astaxanthin, zeaxanthin, lutein, etc.), xanthine derivatives (such as caffeine), proteins or peptides (soy protein, collagen, elastin) , Silk or degradation products thereof), mucopolysaccharides (hyaluronic acid, chondroitin, dermatan, heparan, heparin, ketalan, derivatives thereof, etc.), amino sugars (glucosamine, acetylglucosamine, galactosamine, acetylgalactosamine, Iramamic acid, acetylneuraminic acid, hexosamine, salts thereof, etc., oligosaccharide (isomalto-oligosaccharide, cyclic oligosaccharide, etc.), phospholipid (phosphatidylcholine, phosphatidylserine, etc.), sphingolipid and its derivatives (sphingomyelin, ceramide, etc.) ), Sulfur-containing compounds (such as alliin, sepaene, taurine, glutathione, methylsulfonylmethane), lignans (such as sesamin), pearl powder, α-lipoic acid or derivatives thereof, CoQ10, and animal and plant extracts containing these, root vegetables The denaturation of components such as ginger (ginger etc.) can be prevented. In particular, unstable components that are easily denatured by active oxygen include polyphenols such as proanthocyanidins, gallotannins, chlorogenic acid and derivatives thereof, plant extracts containing these, CoQ10 or derivatives thereof, α-lipoic acid and derivatives thereof, Examples include cysteine or a derivative thereof, thiamine or a derivative thereof, carotenoid or a derivative thereof, tocopherol or a derivative thereof, glutathione and SOD or an extract containing them, lignans, a xanthine derivative, a tomato extract and the like.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited to this range.

(Preparation Example 1)
200 mL of water was added to 5 g of the remaining portion from which the seeds had been removed from the fruit of Terminaria berylica, and the mixture was heated to reflux at 100 ° C. for 30 minutes. After extraction, filtration was performed to obtain a filtrate, and 200 mL of water was further added to the residue, followed by heating under reflux at 100 ° C. for 30 minutes. After extraction, the filtrates obtained by filtration were combined and freeze-dried to obtain extract powder A (2.3 g).

(Preparation Example 2)
Extract powder B (2.4 g) was prepared in the same procedure as in Preparation Example 1 except that 50% by volume ethanol aqueous solution was used in place of the water used in Preparation Example 1 and the heating temperature was 80 ° C. Obtained.

(Preparation Example 3)
Extract powder C (0.4 g) was obtained in the same procedure as in Preparation Example 1 except that 2 g of seed was used instead of the remaining part from which the seed was removed from the fruit used in Preparation Example 1.

(Preparation Example 4)
Extract powder D (0.6 g) was obtained in the same procedure as in Preparation Example 2 except that 2 g of seed was used instead of the remaining part from which the seed was removed from the fruit used in Preparation Example 2.

(Example 1: Evaluation of active oxygen scavenging ability)
Samples were prepared by dissolving the extract powders A to D obtained in Preparation Examples 1 to 4 in water so as to be 3 mg / mL, respectively. Subsequently, SOD activity was measured using a measurement kit (SOD Test Wako, Wako Pure Chemical Industries, Ltd.), and SOD-like activity (unit) per 1 mg of the extract powder was calculated. For comparison, SOD-like activity was calculated using green tea extract (Mitsui Norin Co., Ltd.). The results are shown in Table 1. The measurement result is an average value of triple measurements.

  As shown in Table 1, it is known that all processed products (extract powder) obtained from the fruits of Terminaria berylica have SOD-like activity, particularly in parts other than seeds. It can be seen that it has a much better SOD-like activity than the green tea extract. That is, it can be seen that the processed product obtained from the fruit of Terminaria berylica has excellent active oxygen scavenging ability. Therefore, the active oxygen scavenger of the present invention has an excellent ability to remove active oxygen, and can be used particularly as a food additive.

(Preparation Example 5)
200 mL of water was added to 5 g of the remaining portion from which the seeds had been removed from the fruit of Terminalia chebula, and extraction under heating and reflux was performed at 100 ° C. for 30 minutes. After extraction, filtration was performed to obtain a filtrate, and 200 mL of water was further added to the residue, followed by heating under reflux at 100 ° C. for 30 minutes. After extraction, the filtrates obtained by filtration were combined and freeze-dried to obtain extract powder E (2.3 g).

(Preparation Example 6)
Extract powder F (1.2 g) was obtained in the same procedure as in Preparation Example 5 except that the leaves of Terminalia catappa were used in place of the Terminaria chebra in Preparation Example 5.

(Example 2: Evaluation of active oxygen scavenging ability)
Samples were prepared by dissolving the extract powders E and F obtained in Preparation Examples 5 and 6 in water so as to be 3 mg / mL, respectively. Subsequently, SOD activity was measured using a measurement kit (SOD Test Wako, Wako Pure Chemical Industries, Ltd.), and SOD-like activity (unit) per 1 mg of the extract powder was calculated. For comparison, SOD-like activity was calculated using green tea extract (Mitsui Norin Co., Ltd.). The results are shown in Table 2. The measurement result is an average value of triple measurements.

  As shown in Table 2, the processed product (extract powder) obtained from the fruits of Terminaria chebra and the leaves of Terminaria Katappa was superior to the green tea extract known to have SOD-like activity. It can be seen that it has SOD-like activity. That is, it can be seen that the processed product obtained from the terminaria of the present invention has an excellent active oxygen scavenging ability. Therefore, the active oxygen scavenger of the present invention has an excellent ability to remove active oxygen, and can be used particularly as a food additive.

(Production Example 1)
A beverage (500 mL) was prepared using the following raw materials.

<Blending ingredients>
Extract powder A 0.002 obtained in Preparation Example 1 above
Silk peptide (made by Ichimaru Falcos Co., Ltd.) 0.001
Collagen peptide (Nippi Co., Ltd.) 0.001
Elastin peptide (manufactured by Aswell Corporation) 0.001
Chondroitin complex protein (manufactured by Maruha Corporation) 0.001
Sodium ascorbate (BASF Takeda Vitamin Co., Ltd.) 0.1
Cystine (Ajinomoto Co., Inc.) 0.001
Vitamin B1 (BASF Takeda Vitamin Co., Ltd.) 0.001

(Production Example 2)
A beverage was prepared in the same manner as in Production Example 1 except that Extract Powder E was used instead of Extract Powder A used in Production Example 1.

  The beverages obtained in Production Examples 1 and 2 stably retained ascorbic acid and vitamin B1 that were easily denatured by active oxygen even when stored at 40 ° C. for 1 month.

(Production Example 3)
Tablets (200 mg) were prepared using the following raw materials.

<Blending ingredients> Blending amount (mg)
Extract powder B 30 obtained in Preparation Example 2 above
Pine bark extract (Toyo Shinyaku Co., Ltd.) 30
Sodium ascorbate (BASF Takeda Vitamin Co., Ltd.) 50
Sucrose ester 5.0
Silicon dioxide 2.5
Reduced maltose 82.5

(Production Example 4)
A tablet was prepared in the same manner as in Production Example 3 except that Extract Powder F was used instead of Extract Powder B used in Production Example 3.

  Even when the tablets obtained in Production Examples 3 and 4 were stored at 40 ° C. for 1 month, browning caused by denaturation of pine bark extract and ascorbic acid was not observed.

  ADVANTAGE OF THE INVENTION According to this invention, the active oxygen scavenger which has the outstanding active oxygen scavenging ability consisting of the processed material obtained from terminaria is provided. This active oxygen scavenger is useful, for example, for preventing various diseases caused by superoxide. Furthermore, according to this invention, the food additive which can hold | maintain the component which is easy to modify | denature with active oxygen is provided, and the fall of the quality of a foodstuff can be prevented.

Claims (2)

  An active oxygen scavenger consisting of processed products obtained from Terminaria.   A food additive consisting of processed products obtained from Terminaria.