JP4352030B2 - healthy food - Google Patents

Description

  The present invention relates to a health food containing a processed kuzu flower and γ-aminobutyric acid or a derivative thereof.

  Kuzu is a large vine plant of the legume family, and katsu starch collected from its roots has long been used as a raw material for Japanese confectionery. The roots and flowers are referred to as kudzu and kuzuka, respectively, and are used as raw materials for traditional Chinese medicines such as antipyretic drugs, analgesics, antispasmodics, and drugs for symptoms such as sweating. In particular, unlike other legumes, Kuzuka has been shown to have various effects such as an action for improving liver damage, an action for preventing hangover, and an action for improving urinary nitrogen metabolism (Patent Documents 1 to 3). .

Kuzuka contains various components that may be useful, such as flavonoids. However, since only a limited function has been revealed, it is not fully utilized at present.
Japanese Patent No. 3454718 Japanese Patent Publication No. 8-32632 JP-A 64-68318

  An object of the present invention is to expand the use of Kuzuka by finding a new function of Kuzuka.

  The present inventor has made various studies on the use of kuzuka and found that when the kuzuka processed product and γ-aminobutyric acid or a derivative thereof are combined, they have synergistically excellent anti-obesity and antihypertensive effects. I found it.

  The present invention provides a food containing the processed kuzuka and γ-aminobutyric acid or a derivative thereof.

  Furthermore, the present invention provides an anti-obesity agent containing a processed kuzuka product and γ-aminobutyric acid or a derivative thereof.

  The food and anti-obesity agent of the present invention exhibit excellent anti-obesity and anti-hypertensive effects. Therefore, according to this invention, the use of Kuzuka can be expanded.

  The food and anti-obesity agent of the present invention contain a processed kuzuka product and γ-aminobutyric acid or a derivative thereof, and an additive or the like as necessary. Hereinafter, the components of the food of the present invention will be described individually. The present invention should not be construed as being limited by the following embodiments, and various modifications can be made within the scope of the claims.

(Katsuhana treatment product)
Kuzuka, the flower part of Kuzu, contains flavonoids, saponins, and tryptophan glycosides. Kuzuka includes flowers collected at the stage from buds to fully opened flowers. In the present invention, it is particularly preferable to use soot.

  The processed Kuzuka product used in the food and anti-obesity agent of the present invention is not particularly limited as long as it is a processed Kuzuka product. In the present specification, the “Kuzuhana processed product” refers to a product obtained by subjecting Kuzuka to at least one of a drying process, a pulverizing process, and an extracting process.

  In this specification, in particular, the term “dried kuzuka” refers to a product obtained by drying kuzuka, a dry powder obtained by crushing kuzuka after drying, and the following kuzuka. Contains no flower extract.

  In this specification, when describing as "Kuzuhana extract", the juice of Kuzuka, the extract extracted from Kuzuka, the concentrate obtained by concentrating these juice or extract, the juice of these juices or A dry powder (extract powder) obtained by drying the extract.

  Hereinafter, a method for preparing a kuzu flower dried product, kuzu flower powder (dry powder and extract powder), and a kuzu flower extract, which are processed kuzu flowers, will be described.

  The dried kuzu flower is obtained by drying kuzu flowers, preferably kuzu flowers in the cocoon stage, by sun drying, hot air drying or the like. Preferably, it is dried until the water content is 10% by mass or less.

  Kuzuka powder (dry powder) is obtained by pulverizing the dried Kuzuka product. The powderization is performed by a method commonly used by those skilled in the art, for example, a ball mill, a hammer mill, a roller mill or the like.

  Alternatively, the Kuzuka powder (dried powder) is obtained by crushing the collected Kuzuka using a mass colloider, a slicer, a Komitrol, etc. to obtain a Kuzuka crushed product, and drying the Kuzuka crushed product. .

  The kuzu flower extract is prepared by adding a solvent to a kuzu flower dried product such as kuzu flower, kuzu flower crushed product, kuzu flower dried product, or kuzu flower powder (dried powder), preferably kuzu flower dried powder, for example. Accordingly, the extraction is performed by heating, and the extract is collected by centrifugation or filtration.

  Examples of the solvent that can be used to obtain the Kuzuhana extract include water, organic solvents, and hydrous organic solvents. Examples of the organic solvent include methanol, ethanol, n-propanol, n-butanol, acetone, hexane, cyclohexane, propylene glycol, ethyl methyl ketone, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible oils and fats, 1, Examples include 1,1,2-tetrafluoroethane, 1,1,2-trichloroethene and the like. Among these, a polar organic solvent is preferable, ethanol, n-butanol, methanol, acetone, propylene glycol, and ethyl acetate are more preferable, and ethanol is most preferable.

  Examples of the extraction method include a warm extraction method such as heating reflux, a supercritical extraction method, and the like. In these extraction methods, heating may be performed by applying pressure as necessary. When heating, it is necessary to prevent the solvent added to Kuzuka from volatilizing. When heating, the extraction temperature is preferably 50 ° C. or higher, more preferably 70 ° C. or higher, preferably 130 ° C. or lower, more preferably 100 ° C. or lower.

  The extraction time may be a time for sufficiently extracting soluble components from the extraction raw material, and may be set as appropriate according to the extraction temperature and the like. Preferably, it is 30 minutes to 48 hours. For example, when the extraction temperature is less than 50 ° C., it can be 6 hours to 48 hours, and when it is 50 ° C. or more, it can be 30 minutes to 24 hours.

  The obtained extract may be concentrated or dried by a method such as concentration under reduced pressure or freeze-drying, if necessary, to form a liquid, a paste, or a powder (extract powder).

  The processed Kuzuka product thus obtained may contain isoflavones, saponins and the like. The isoflavones contained in the processed kuzuhana product are preferably 3% by mass or more, more preferably 5% by mass to 90% by mass with respect to the dry mass of the extract. The saponin contained is preferably 1% by mass or more, more preferably 2% by mass to 50% by mass.

(Γ-aminobutyric acid or its derivative)
It is known that γ-aminobutyric acid is a kind of amino acid, plays an important function as a signal transmitter in the brain, and is involved in metabolic regulation in the brain. In recent years, it has also been revealed that γ-aminobutyric acid has an antihypertensive action, and it has begun to be used as a food and medicine for the purpose of preventing hypertension. Furthermore, since it is possible to produce γ-aminobutyric acid from glutamic acid by glutamic acid decarbonase present in plants, it has begun to be used as a functional component contained in plants.

  Examples of derivatives of γ-aminobutyric acid include salts and esters of γ-aminobutyric acid. Specific examples include nicotinoyl γ-aminobutyric acid, calcium salt of γ-aminobutyric acid, methyl ester, ethyl ester, propyl ester, isopropyl ester, butyl ester, tert-butyl ester and the like of γ-aminobutyric acid. In the present invention, a raw material containing these components may be used. Examples of such a raw material include a plant containing γ-aminobutyric acid or a derivative thereof or a processed product thereof (squeezed juice such as tea, wheat young leaves, kale or the extract thereof, dried powder, germinated brown rice, etc.).

(Other additives)
The food and anti-obesity agent of the present invention include excipients, extenders, binders, thickeners, emulsifiers, colorants, fragrances, other food ingredients, seasonings, pharmaceutical ingredients, etc., as necessary. May be added. Furthermore, you may shape | mold into forms, such as a granule and a tablet, according to a use. The form of the food and anti-obesity agent of the present invention is not particularly limited, but may be formed into capsules such as hard capsules and soft capsules, tablets, or pills as necessary, or in powder form or granule form Shape, tea shape, tea bag shape, bowl shape, or the like, or a beverage form as it is. Depending on these shapes or preferences, the food and anti-obesity agent of the present invention may be eaten as they are, or may be taken by dissolving in water, hot water, milk or the like. For example, it may be pulverized to form a tea bag, and the ingredients may be leached before drinking.

  Examples of food materials that can be added to the food and anti-obesity agent of the present invention include royal jelly, propolis, vitamins (A, C, D, E, K, folic acid, pantothenic acid, biotin, derivatives thereof, etc.), minerals (Iron, magnesium, calcium, zinc, selenium, etc.), α-lipoic acid, lecithin, polyphenols (flavonoids, derivatives thereof, etc.), carotenoids (lycopene, astaxanthin, zeaxanthin, lutein, etc.), xanthine derivatives (caffeine, etc.) Fatty acids, proteins (collagen, elastin, etc.), mucopolysaccharides (hyaluronic acid, etc.), amino sugars (glucosamine, acetylglucosamine, galactosamine, acetylgalactosamine, neuraminic acid, acetylneuraminic acid, hexosamine, salts thereof, etc.), oligo sugar Isomalt-oligosaccharides, cyclic oligosaccharides, etc.), phospholipids and their derivatives (phosphatidylcholine, sphingomyelin, ceramide, etc.), sulfur-containing compounds (eg, alliin, sepaene, taurine, glutathione, methylsulfonylmethane), sugar alcohols, lignans (Such as sesamin), animal and plant extracts containing these, root vegetables (such as turmeric and ginger), and the like.

  In particular, if a lipid absorption inhibitory component and / or a lipid metabolism promoting component is added to the food and anti-obesity agent of the present invention, a lipid accumulation inhibitory effect or a body fat reduction effect can be obtained synergistically. As a result, it can be expected to prevent various symptoms caused by obesity (for example, diabetes).

  Examples of the lipid absorption inhibitory component include components having an action of excreting bile acids (chitosan and its derivatives, psyllium, proanthocyanidins and the like) and components having a lipase inhibitory action (gallotannins and the like). In this invention, you may use the raw material containing these components instead of these components. Examples of such raw materials include plant extracts and loquat leaves, and preferably pine bark extracts and loquat leaf extracts.

  Examples of lipid metabolism promoting components include riboflavin, tea catechins, isomerized linoleic acid, caffeine, capsaicin, carnitine, coenzyme Q10, soybean peptide, branched amino acid, phosphatidylcholine, allyl sulfide compound, forskolin, bergenin, quercetin, astilbine, Examples include hydroxycitric acid and salts thereof. In this invention, you may use the raw material containing these components instead of these components. Examples of such raw materials include extracts such as tea, coleus foliage, red pepper, yellow potato, soybean, chili, buckwheat, garlic, onion, and coffee.

  The lipid absorption suppressing component and the lipid metabolism promoting component are appropriately blended in the food and anti-obesity agent of the present invention according to the purpose. These components may be used alone or in combination of two or more.

(Anti-obesity agent)
The anti-obesity agent of the present invention contains a processed kuzuka product and γ-aminobutyric acid or a derivative thereof, and contains additives as necessary.

  There is no restriction | limiting in particular in content of each of these components. For example, the content of Kuzuka extract (dry mass) is preferably formulated so that the intake of Kuzuka extract (dry mass) per adult day is preferably 10 mg or more, preferably Is preferably not more than 3000 mg, more preferably not more than 1000 mg.

  In the case of dried katsuhana, the amount of dried kuzuka per day is preferably 0.1 g or more, preferably 30 g or less, more preferably 10 g or less. It is good to mix | blend so that it may become.

  Since the content of the processed kuzuhana product differs depending on the form or dosage form, it may be appropriately adjusted. For example, in the case of Kuzuhana extract, the Kuzuka extract (dry mass) in the anti-obesity agent is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, preferably Is 50% by mass or less, more preferably 30% by mass or less. In the case of dried katsuhana, the dried kuzuka is preferably 0.01% by mass or more, more preferably 0.1% by mass or more, preferably 80% by mass or less. More preferably, it is good to set it as 50 mass% or less.

  The content of γ-aminobutyric acid or a derivative thereof in the anti-obesity agent of the present invention is not particularly limited. For example, although depending on the dosage form, in the anti-obesity agent, γ-aminobutyric acid or a derivative thereof is preferably 0.01% by mass or more, and γ-aminobutyric acid or a derivative thereof is preferably 50% or more. It is good to be less than mass%.

  There are no particular limitations on the blending ratio of the processed kuzuka product and γ-aminobutyric acid or its derivative. For example, in the case of Kuzuka extract, γ-aminobutyric acid or a derivative thereof is preferably 0.01 parts by mass or more, more preferably 0.1 parts by mass or more with respect to 100 parts by mass of Kuzuka extract. Preferably, it is 300 parts by mass or less, more preferably 200 parts by mass or less.

(Food)
The food of the present invention contains a processed kuzuka product and γ-aminobutyric acid or a derivative thereof, and contains additives and the like as necessary. Preferably, the above anti-obesity agent can be used as the food of the present invention. Since the food of the present invention exhibits an antihypertensive effect in addition to the excellent antiobesity effect, it can also be used as an antihypertensive agent.

  The content of the processed Kuzuka product and γ-aminobutyric acid or a derivative thereof in the anti-obesity agent of the present invention is not particularly limited. Therefore, the content of the processed Kuzuka product and γ-aminobutyric acid or a derivative thereof may be within the above range.

  Furthermore, the food of the present invention may be in the form of a beverage such as fermented plant juice, vegetable juice (eg, carrot juice), plant extract, fruit juice and the like containing the anti-obesity agent of the present invention. By setting it as such a form, not only the functionality or nutritional value of these drinks is improved, but also palatability is improved. These beverages can be enhanced in sweetness by adding sugar solution, sugar alcohol, seasoning or the like.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited to this range.

(Example 1: Examination of anti-obesity effect)
About the foodstuff of this invention, the effect with respect to a body weight increase was examined with the following method.

  5% by mass of kuzuhana extract (containing 10% by mass of isoflavones and 1% by mass of saponin: Ota Gassan Co., Ltd.) and 0.3% by mass of γ-aminobutyric acid-containing rice germ extract (5% by mass in the extract) Of γ-aminobutyric acid, 0.015% by mass of γ-aminobutyric acid based on the whole feed: Oriza Oil Chemical Co., Ltd.), 40% by weight of beef tallow, and 9% by mass of granulated sugar Thus, test feed 1 was prepared by adding to the standard feed.

  Comparative feed 1 was prepared in the same procedure as test feed 1 except that the kuzu flower extract was not added.

  Control feed 1 was prepared in the same procedure as test feed 1 except that the kuzu flower extract and the γ-aminobutyric acid-containing rice germ extract were not added.

  Twelve 8-week-old female ICR mice (Nippon Charles River Co., Ltd.) were fed with a standard diet (MF feed, Oriental Yeast Co., Ltd.) for one week. Then, it was divided into 3 groups of 4 animals per group so that the average body weight per group was uniform.

  One group of mice received test feed 1, the other group received comparative feed 1, and the other group of mice received control feed 1 ad libitum.

  On the 25th day from the start of free intake, the weight of each mouse was measured. After the measurement, the weight gain rate (%) was calculated from the following formula (I). The results are shown in Table 1.

  As shown in Table 1, the mice in the group that ingested the test feed 1 were found to have reduced body weight gain compared to the mice in the group that ingested the comparative feed 1 and the mice in the group that received the control feed 1. Recognize. Therefore, it turns out that the foodstuff of this invention has an anti-obesity action.

(Example 2: Examination of body fat reduction effect)
Regarding the food of the present invention, the influence on fat accumulation in subcutaneous fat was examined by the following method.

  Twenty-one 7-week-old female ICR mice (Nippon Charles River Co., Ltd.) were fed with a standard diet (MF feed, Oriental Yeast Co., Ltd.) for one week. Subsequently, it was divided into 3 groups of 7 animals per group so that the average body weight per group was uniform.

  Then, one group of mice received the test feed 1 freely, the other group of mice received the comparative diet 1 and the remaining one group of mice received the control diet 1 freely.

  As shown in Table 2, the accumulation of subcutaneous fat was suppressed in the mice in the group that received the test diet 1 compared to the mice in the group that received the comparative diet 1 and the mice in the group that received the control diet 1 . Therefore, it can be seen that the food of the present invention has an excellent body fat reducing action.

  As mentioned above, it turns out that the foodstuff of this invention prevents obesity by reducing a body fat, and has the outstanding anti-obesity action.

(Example 3: Examination of antihypertensive effect)
About the foodstuff of this invention, the influence with respect to blood pressure was examined with the following method.

  A rice germ extract containing 10% by weight of kuzuhana extract (containing 10% by weight of isoflavones and 1% by weight of saponin: Ota Sakusan Co., Ltd.) and γ-aminobutyric acid (5% by weight of γ-aminobutyric acid in the extract) Containing 0.003% by mass of γ-aminobutyric acid with respect to the total feed: Oriza Oil Chemical Co., Ltd.) is added to the standard feed so as to contain 0.06% by mass, and test feed 2 is prepared. did.

  Moreover, the control feed 2 was prepared by adding the kuzu flower extract to the standard feed at a ratio of 1% by mass.

  Twelve 5-week-old spontaneously hypertensive (SHR) rats (Nippon Charles River Co., Ltd.) were fed with a standard feed (MF feed, Oriental Yeast Co., Ltd.) for one week. Then, the group was divided into two groups of six per group so that the average blood pressure per group was uniform.

  A group of rats was then given free access to test feed 2 and the remaining group of rats was given control feed 2 ad libitum.

  On the 28th day from the start of free intake, the systolic blood pressure of the tail of each rat was measured. The results are shown in Table 3.

  As shown in Table 3, the systolic blood pressure of the rats fed the test diet 2 was lower than the systolic blood pressure of the rats fed the control diet 2. Therefore, it turns out that the foodstuff of this invention shows the outstanding antihypertensive effect by ingesting continuously.

(Production Example 1)
Tablets (200 mg per tablet) were produced using the following raw materials.

<Tablet ingredients> Amount (% by mass)
Kuzuhana extract (Ota gassan) 5
Pine bark extract (Toyo Shinyaku Co., Ltd.) 1
Ascorbic acid 10
Crystalline cellulose 14
Sucrose ester 4
Reduced maltose 30
Silicon dioxide 1
Trehalose 25
Barley young leaf powder containing 1% by weight of γ-aminobutyric acid 10

(Production Example 2)
Granules were produced using the following raw materials.

<Blend ingredients> Blending amount (% by mass)
Kuzuhana extract 2
Soy peptide 25
Tea catechin 10
Crystalline cellulose 10
Reduced maltose 20
Kale dry powder containing 1% by mass of γ-aminobutyric acid 33

  Since the food and anti-obesity agent of the present invention exhibit excellent anti-obesity and anti-hypertensive effects, they are useful for preventing various symptoms caused by obesity and hypertension.

Claims (1)

  An anti-obesity agent comprising a processed kuzu flower and γ-aminobutyric acid or a derivative thereof.