JP2006160655A - Muscular activator - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an ameliorating agent for a change in skin conditions according to aging by promoting energy production in myocytes and activating the muscle. <P>SOLUTION: The ameliorating agent for the change in the skin conditions effective in ameliorating weakening of the skin according to the aging is obtained by including an extract from Glycyrrhiza glabra Linne or Glycyrrhiza uralensis Fisher or other plants of the same genus. The ameliorating agent for the skin conditions comprising the extract from the Glycyrrhiza glabra Linne or Glycyrrhiza uralensis Fisher ameliorates the change in the skin conditions according to the aging by promoting the energy production in the myocytes and activating the muscle. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a muscle activator characterized by containing an extract of licorice, and more particularly to a muscle activator that exhibits effectiveness in improving changes in skin properties due to aging.

  Muscle regulates the contraction and relaxation of the skin by its contraction function. On the face, the subcutaneous muscles are in close contact with the skin, preventing the skin from descending due to gravity and maintaining the beam directly. Conventionally, it is well known that aging causes changes in skin wrinkles and sagging. It is known that muscular function is also reduced by aging, and it is considered that the formation of wrinkles and sagging is deeply related to the hypodermic dysfunction as well as changes in the epidermis and dermis.

Muscle contraction occurs when the actin fibers slide on the myosin fibers using the energy associated with the hydrolysis of ATP (Non-patent Document 1). Therefore, in order to prevent a decrease in muscle function, it is considered effective to promote energy production in the cells constituting the muscle and to activate the muscle. Has been reported. On the other hand, muscle cell energy production promoting effect of licorice extract is not known.
Original Book 13th edition, Medical Physiology Perspective p53-64 Maruzen JP2001-10947

  An object of the present invention is to suppress or improve changes in skin atrophy, wrinkles, sagging, etc. caused by aging by promoting energy production in muscle cells and activating muscles.

  Under these circumstances, as a result of intensive research, the present inventors have found that licorice extract promotes energy production in muscle cells and activates muscles, thereby preventing changes in skin properties associated with aging. The present inventors have found that it has an excellent improvement effect and have completed the present invention.

  The licorice used in the present invention refers to a plant belonging to the Leguminosae family, and has a scientific name of Glycyrrhiza glabra Linne, Glycyrrhiza uralensis Fisher, or other related genera. It has been used as a medicinal plant since 4000 years ago, and its records remain in Assyrian tablets (paques) and Egyptian papyrus. Since dried roots and rhizomes are distributed as crude drugs, they can be used.

  The extraction method of licorice used by this invention is not specifically limited, For example, what was extracted by heating may be used, and what was extracted at normal temperature or low temperature may be used. Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol). , Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) Etc.). Preferred are polar solvents such as lower alcohols and liquid polyhydric alcohols, and particularly preferred are ethanol, 1,3-butylene glycol, and propylene glycol. The above solvents may be used alone or in combination of two or more, and may be used as a hydrous solvent.

  The extract may be used as it is, or may be used after concentration, dilution, filtration, decolorization with activated carbon, deodorization, or the like, if necessary. Further, the extracted solution may be used as a dried product after being concentrated and dried, spray dried, freeze dried, etc., or may be used after concentrating or isolating the active ingredient by performing column purification or the like. good. Moreover, a commercial item can also be used for these extracts and refined products. This extract may be used as it is, and within the range that does not impair the effect of the extract, oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjustment Components such as agents, preservatives, fragrances, moisturizers, powders, ultraviolet absorbers, thickeners, dyes, antioxidants, whitening agents, chelating agents and the like can also be blended.

  The muscle activator of the present invention and the energy production promoter in muscle cells are usually administered systemically or locally. Examples of the administration method include oral administration and transdermal administration. The dose varies depending on age, body weight, symptoms, therapeutic effect, administration method, treatment time, etc., but is usually 1 mg to 1 g per adult, preferably 20 mg to 200 mg once to several times a day. Is done. Since the dosage varies depending on various conditions, an amount smaller than the above-mentioned administration range may be sufficient, or it may be necessary to administer beyond the range.

  The muscle activator used in the present invention and the energy production promoter in muscle cells can be used for any of foods, cosmetics, quasi drugs, and pharmaceuticals. Examples of the dosage form include tablet confectionery, beverages, Lotion, cream, emulsion, gel, aerosol, ointment, poultice, paste, plaster, essence, pack, cleaning agent, bath preparation, foundation, powder, lipstick, powder, pill, tablet, injection, Suppositories, emulsions, capsules, granules, liquids (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.).

  The blending amount of the muscle activating agent and the energy production promoter in muscle cells used in the present invention is not particularly limited, but is preferably in the range of 0.0001 to 75% by weight, more preferably 0.001 as a dried product of licorice extract. -30% by weight. If it is 0.0001% by weight or less, the effect is low, and even if it exceeds 75% by weight, the effect is hardly increased and it is not efficient. The addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.

  That is, the present invention relates to a muscle activator characterized by containing an extract of licorice, an energy production promoter in muscle cells, and an anti-aging agent for skin.

  Next, in order to describe the present invention in detail, examples of production of the extract used in the present invention, formulation examples and experimental examples will be given as examples, but the present invention is not limited thereto. The compounding amount shown in the examples indicates% by weight.

Production Example 1 Licorice Ethanol Extract 800 g of 80% ethanol was added to 100 g of licorice root, extracted at room temperature for 7 days, insoluble matter was filtered, the filtrate was concentrated to dryness, and 8.5 g of licorice ethanol extract was obtained. Got.

Production Example 2 Extract of licorice 1,3-butylene glycol aqueous solution To 25 g of licorice root and stem, 200 g of purified water and 200 g of 1,3-butylene glycol were added and kept at 80 ° C. for 3 hours with stirring, followed by filtration. 380 g of 1,3-butylene glycol aqueous solution extract was obtained.

Production Example 3 Hot Water Extract of Licorice 400 mL of purified water was added to 20 g of licorice root, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried and hot water extract of licorice 2.2g was obtained.

Production Example 4 Licorice ethyl ether extract 800 mL of ethyl ether was added to 100 g of licorice root, extracted at room temperature for 7 days, insoluble matter was filtered, the filtrate was concentrated to dryness, and licorice ethyl ether extract 1. 5 g was obtained.

  Next, the prescription of the Example which concerns on this invention is shown.

Formulation Example 1 Cream Formulation Amount (% by weight)
1. Licorice ethanol extract (Production Example 1) 0.05
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11.1,3-butylene glycol 8.5
12 Ethyl paraoxybenzoate 0.05
13. Methyl paraoxybenzoate 0.2
14 Purified water 68.1
[Manufacturing method] Components 1 to 9 are heated and mixed to maintain an oil phase at 70 ° C. Ingredients 11-14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Comparative Example 1 Conventional Cream A conventional cream was prepared by replacing the ethanol extract of licorice with purified water in Formulation Example 1.

Formulation Example 2 Lotion Formulation Amount (% by weight)
1. Licorice 1,3-butylene glycol aqueous solution extract (Production Example 2) 0.1
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Fragrance 0.1
11. Purified water 84.47
[Production method] Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved, and both are mixed and filtered to obtain a product.

Formulation Example 3 Emulsion Formulation Amount (wt%)
1. Licorice ethyl ether extract (Production Example 4) 1.0
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Purified water 72.2
[Manufacturing method] Components 1 to 8 are dissolved by heating and mixed to maintain an oil phase at 70 ° C. Ingredients 10 to 13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.

Formulation Example 4 Tablet Confectionery Formulation Amount (wt%)
1. Licorice hot water extract (Production Example 3) 2.0
2. Dried corn starch 50.0
3. Erythritol 40.0
4). Citric acid 5.0
5. Sucrose fatty acid ester 3.0
6). Perfume appropriate amount 7. Water Appropriate amount [Production method] Components 1 to 4 and 7 are mixed and granulated. Ingredients 5 and 6 are added to the formed granules and compressed. One tablet is 1.0 g.

Formulation Example 5 Tablet formulation Formulation amount (% by weight)
1. Licorice ethanol extract (Production Example 1) 10.0
2. Corn starch 10.0
3. Purified white sugar 20.0
4). Carboxymethylcellulose 10.0
5. Microcrystalline cellulose 35.0
6). Polyvinylpyrrolidone 5.0
7). Talc 10.0
[Production Method] Components 1 to 5 were mixed, and then an aqueous solution of Component 6 was added as a binder and granulated by a conventional method. This was mixed with ingredient 7 as a lubricant and then tableted into 100 mg tablets.

  Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 Energy production promoting effect in muscle cells by licorice extract Muscle-derived cell line C2C12 cells were cultured in a DMEM culture solution containing 10% fetal bovine serum under conditions of 37 ° C. and 5% CO ₂ . In a confluent state, the sample was added to a DMEM culture solution containing 0.75% fetal calf serum added to a final concentration of 1 μg / mL as a solid substance at 37 ° C. under 5% CO ₂ for another 24 hours. Cultured. After culturing, the cells were detached by trypsin treatment, the number of cells was counted using a hemocytometer, and the amount of ATP in the cells was measured using Lucifer 250 (Kikkoman). That is, 0.1 mL of cell suspension was placed in a measurement tube, 0.1 mL of ATP extraction reagent was added thereto, and after 20 seconds, 0.1 mL of a luminescent reagent was added, stirred with a vortex mixer, The amount of luminescence was measured with LIGHT 55). The amount of ATP in the sample was calculated from the calibration curve obtained with the ATP standard, and the amount of ATP per cell was calculated from the result of the number of cells obtained previously. The ATP production rate when the sample was added was determined as a value when the amount of ATP when the sample was not added was taken as 100.

The test results are shown in Table 1. As a result, the licorice extract was confirmed to have an effect of promoting ATP production in C2C12 cells. In particular, the effect of ethanol and 1,3-butylene glycol aqueous solution extract was high.

Experiment 2 Use test 1
4 months use for 30 women (33-58 years old) who feel skin changes due to aging using 50% ethanol aqueous solution containing 1% licorice ethanol extract (Production Example 1) A test was conducted. After use, the effect of improving skin sagging, wrinkles and beams was judged by a questionnaire.

  These results are shown in Table 2. When the ethanol extract of 1% licorice was used, the skin sagging, wrinkle and elasticity were improved more than when the base alone was used as a control. During the test period, there was no skin problem and there was no problem with safety.

Experiment 3 Use test 2
Using the cream of Formulation Example 1 and the conventional cream of Comparative Example 1, a use test for 3 months was conducted on 40 women (29 to 60 years old) who each felt skin changes due to aging. After use, the effect of improving skin sagging, wrinkles and beams was judged by a questionnaire.

  These results are shown in Table 3. The skin external preparation characterized by containing the licorice ethanol extract of Formulation Example 1 showed an excellent effect. During the test period, there was no skin problem and there was no problem with safety.

  When the usage test of the skin lotion of Formulation Example 2, the emulsion of Formulation Example 3, the tablet confection of Formulation Example 4 and the tablet of Formulation Example 5 was conducted, all of them were safe and had an excellent improvement effect on sagging skin, wrinkles, and elasticity. Indicated.

As an application example of the present invention, it can be used for any of foods, cosmetics, quasi drugs and pharmaceuticals. Examples of the dosage form include skin lotion, cream, milky lotion, tablet confectionery, tablet and the like, and can be used as an excellent muscle activator by external application or internal use. In addition, it is possible to provide a skin anti-aging agent that is safe and has an excellent effect of improving changes in skin properties due to aging.

Claims (3)

  A muscle activator comprising an extract of licorice.   An agent for promoting energy production in muscle cells, comprising an extract of licorice. A skin anti-aging agent comprising the muscle activator according to claim 1 or the energy production promoter in muscle cells according to claim 2.