JP2006042820A5 - - Google Patents
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- Publication number
- JP2006042820A5 JP2006042820A5 JP2005223872A JP2005223872A JP2006042820A5 JP 2006042820 A5 JP2006042820 A5 JP 2006042820A5 JP 2005223872 A JP2005223872 A JP 2005223872A JP 2005223872 A JP2005223872 A JP 2005223872A JP 2006042820 A5 JP2006042820 A5 JP 2006042820A5
- Authority
- JP
- Japan
- Prior art keywords
- fusion protein
- purifying
- glutathione
- recovered
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020001507 fusion proteins Proteins 0.000 claims description 3
- 102000037865 fusion proteins Human genes 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 2
- 239000003945 anionic surfactant Substances 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 235000019626 lipase activity Nutrition 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- 108090000190 Thrombin Proteins 0.000 description 3
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 3
- 108010024636 Glutathione Proteins 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 229960004072 thrombin Drugs 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 101710098556 Lipase A Proteins 0.000 description 1
- 101710099648 Lysosomal acid lipase/cholesteryl ester hydrolase Proteins 0.000 description 1
- 102100026001 Lysosomal acid lipase/cholesteryl ester hydrolase Human genes 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- PXXJHWLDUBFPOL-UHFFFAOYSA-N benzamidine Chemical compound NC(=N)C1=CC=CC=C1 PXXJHWLDUBFPOL-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI20043110A MY136932A (en) | 2004-08-02 | 2004-08-02 | Novel geobacillus microorganism |
| MYPI20043110 | 2004-08-02 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006042820A JP2006042820A (ja) | 2006-02-16 |
| JP2006042820A5 true JP2006042820A5 (enExample) | 2008-07-03 |
| JP4912636B2 JP4912636B2 (ja) | 2012-04-11 |
Family
ID=35219679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005223872A Expired - Fee Related JP4912636B2 (ja) | 2004-08-02 | 2005-08-02 | 新規なジオバチルス微生物 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7319029B2 (enExample) |
| EP (1) | EP1624056B1 (enExample) |
| JP (1) | JP4912636B2 (enExample) |
| DE (1) | DE602005011079D1 (enExample) |
| DK (1) | DK1624056T3 (enExample) |
| MY (1) | MY136932A (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007114324A1 (ja) * | 2006-03-31 | 2007-10-11 | Menicon Co., Ltd. | バイオマス処理方法、堆肥、畜産用敷料、及びバイオマス用処理剤 |
| MY154817A (en) * | 2006-06-21 | 2015-07-31 | Univ Putra Malaysia | Cystallization of enzyme and method for producing same |
| DE602006017710D1 (en) | 2006-09-14 | 2010-12-02 | Tmo Renewables Ltd | Lipase |
| EP2516611A1 (en) * | 2009-12-21 | 2012-10-31 | Danisco US Inc. | Detergent compositions containing geobacillus stearothermophilus lipase and methods of use thereof |
| WO2012150976A2 (en) * | 2011-02-04 | 2012-11-08 | North Carolina State University | Methods and compositions for the production of extremophile enzymes from green microalgae and cyanobacteria |
| CN102732490A (zh) * | 2011-04-01 | 2012-10-17 | 武汉新华扬生物股份有限公司 | 一种温度适性广的中性脂肪酶lipg及其基因和应用 |
| US10196588B2 (en) * | 2012-05-02 | 2019-02-05 | Universiti Putra Malaysia | Detergent formulation for dishwashing machine |
| CN102876643A (zh) * | 2012-09-21 | 2013-01-16 | 中国农业科学院饲料研究所 | Gdsl蛋白在制备脂肪酶中的新用途 |
| WO2014069986A1 (en) * | 2012-10-30 | 2014-05-08 | Universiti Putra Malaysia | Use of a newly isolated yeast in the expression of recombinant proteins |
| EP2806030A1 (en) * | 2013-05-20 | 2014-11-26 | Universiti Putra Malaysia | A process for producing lipase |
| RU2540873C1 (ru) * | 2013-09-23 | 2015-02-10 | Федеральное государственное бюджетное учреждение науки Институт цитологии и генетики Сибирского отделения Российской академии наук (ИЦиг СО РАН) | ШТАММ БАКТЕРИИ Escerichia coli XL1-blue/pQS-G3, ПРОДУЦЕНТ ТЕРМОСТАБИЛЬНОЙ ЛИПАЗЫ БАКТЕРИИ Geobacillus stearothermophilus G3 |
| WO2017069606A2 (en) * | 2015-10-23 | 2017-04-27 | Myenzyme Sdn Bdh | Thermolabile enzyme and method of preparing thereof |
| CN106868026A (zh) * | 2017-03-22 | 2017-06-20 | 东莞泛亚太生物科技有限公司 | 用于生产具有耐温性脂肪酶的基因 |
| CN107475145B (zh) * | 2017-04-21 | 2021-05-25 | 宜春学院 | 产耐中高温纤维素酶菌株及其筛选方法 |
-
2004
- 2004-08-02 MY MYPI20043110A patent/MY136932A/en unknown
-
2005
- 2005-03-16 DE DE602005011079T patent/DE602005011079D1/de not_active Expired - Fee Related
- 2005-03-16 DK DK05005743T patent/DK1624056T3/da active
- 2005-03-16 EP EP05005743A patent/EP1624056B1/en not_active Expired - Lifetime
- 2005-07-29 US US11/193,282 patent/US7319029B2/en not_active Expired - Lifetime
- 2005-08-02 JP JP2005223872A patent/JP4912636B2/ja not_active Expired - Fee Related
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