JP2005537030A - 核酸を分析する方法 - Google Patents
核酸を分析する方法 Download PDFInfo
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- JP2005537030A JP2005537030A JP2004555256A JP2004555256A JP2005537030A JP 2005537030 A JP2005537030 A JP 2005537030A JP 2004555256 A JP2004555256 A JP 2004555256A JP 2004555256 A JP2004555256 A JP 2004555256A JP 2005537030 A JP2005537030 A JP 2005537030A
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| JP2010522569A (ja) * | 2007-03-28 | 2010-07-08 | バイオナノマトリックス、インク． | ナノチャネルアレイを用いた巨大分子解析方法 |
| JP2010233496A (ja) * | 2009-03-31 | 2010-10-21 | Sekisui Medical Co Ltd | Ｕｇｔ１ａ１遺伝子多型検出法 |
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| US6696022B1 (en) | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
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| US20020187508A1 (en) * | 2001-06-08 | 2002-12-12 | Wong Gordon G. | Methods and products for analyzing nucleic acids using nick translation |
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| WO2007011660A2 (en) * | 2005-07-14 | 2007-01-25 | William Marsh Rice University | Quantum dot probes |
| US20070128083A1 (en) * | 2005-07-18 | 2007-06-07 | U.S. Genomics, Inc. | Microfluidic methods and apparatuses for sample preparation and analysis |
| JP4767654B2 (ja) * | 2005-10-21 | 2011-09-07 | 株式会社エヌ・ティ・ティ・ドコモ | 分子伝送・分子配送システムおよび分子伝送・分子配送方法 |
| US20080241071A1 (en) * | 2006-07-14 | 2008-10-02 | West Jennifer L | Quantum Dot Probes |
| EP3978121A1 (en) | 2006-07-19 | 2022-04-06 | BioNano Genomics, Inc. | Nanonozzle device arrays: their preparation and use for macromolecular analysis |
| US20080066549A1 (en) * | 2006-09-18 | 2008-03-20 | Oldham Mark F | Methods, systems and apparatus for light concentrating mechanisms |
| WO2008085991A2 (en) | 2007-01-08 | 2008-07-17 | U.S. Genomics, Inc. | Reaction chamber |
| US8628919B2 (en) | 2008-06-30 | 2014-01-14 | Bionano Genomics, Inc. | Methods and devices for single-molecule whole genome analysis |
| US8361716B2 (en) | 2008-10-03 | 2013-01-29 | Pathogenetix, Inc. | Focusing chamber |
| CN108467887A (zh) | 2008-11-18 | 2018-08-31 | 生物纳米基因公司 | 多核苷酸作图和测序 |
| US8738296B2 (en) * | 2009-02-03 | 2014-05-27 | Complete Genomics, Inc. | Indexing a reference sequence for oligomer sequence mapping |
| EP2394165A4 (en) * | 2009-02-03 | 2013-12-11 | Complete Genomics Inc | ASSIGNMENT OF OLIGOMER SEQUENCES |
| EP2394164A4 (en) * | 2009-02-03 | 2014-01-08 | Complete Genomics Inc | ASSIGNMENT OF OLIGOMER SEQUENCES |
| EP2511843B1 (en) * | 2009-04-29 | 2016-12-21 | Complete Genomics, Inc. | Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US9028776B2 (en) | 2012-04-18 | 2015-05-12 | Toxic Report Llc | Device for stretching a polymer in a fluid sample |
| US8956815B2 (en) | 2012-04-18 | 2015-02-17 | Toxic Report Llc | Intercalation methods and devices |
| US8685708B2 (en) | 2012-04-18 | 2014-04-01 | Pathogenetix, Inc. | Device for preparing a sample |
| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| US10726942B2 (en) | 2013-08-23 | 2020-07-28 | Complete Genomics, Inc. | Long fragment de novo assembly using short reads |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CN109922430B (zh) * | 2019-03-20 | 2020-12-18 | 苏州真趣信息科技有限公司 | 受限空间作业系统及方法 |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| ES2982420T3 (es) | 2019-12-23 | 2024-10-16 | 10X Genomics Inc | Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| ES2994976T3 (en) | 2020-06-25 | 2025-02-05 | 10X Genomics Inc | Spatial analysis of dna methylation |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4482979B1 (en) | 2022-11-09 | 2025-10-01 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US6007987A (en) * | 1993-08-23 | 1999-12-28 | The Trustees Of Boston University | Positional sequencing by hybridization |
| FR2716263B1 (fr) * | 1994-02-11 | 1997-01-17 | Pasteur Institut | Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon. |
| US20010055812A1 (en) * | 1995-12-05 | 2001-12-27 | Alec Mian | Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics |
| WO1998035012A2 (en) * | 1997-02-12 | 1998-08-13 | Chan Eugene Y | Methods and products for analyzing polymers |
| US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
| US6210896B1 (en) * | 1998-08-13 | 2001-04-03 | Us Genomics | Molecular motors |
| US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US6927065B2 (en) * | 1999-08-13 | 2005-08-09 | U.S. Genomics, Inc. | Methods and apparatus for characterization of single polymers |
| US6696022B1 (en) * | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
| EP1265709A2 (en) * | 2000-03-17 | 2002-12-18 | Aclara BioSciences, Inc. | Microfluidic device and system with additional peripheral channels |
| US20060008799A1 (en) * | 2000-05-22 | 2006-01-12 | Hong Cai | Rapid haplotyping by single molecule detection |
| US20020187508A1 (en) * | 2001-06-08 | 2002-12-12 | Wong Gordon G. | Methods and products for analyzing nucleic acids using nick translation |
| US20020197639A1 (en) * | 2001-06-08 | 2002-12-26 | Shia Michael A. | Methods and products for analyzing nucleic acids based on methylation status |
| WO2003025540A2 (en) * | 2001-09-18 | 2003-03-27 | U.S. Genomics, Inc. | Differential tagging of polymers for high resolution linear analysis |
| US8423294B2 (en) * | 2001-09-18 | 2013-04-16 | Pathogenetix, Inc. | High resolution linear analysis of polymers |
| US20030215864A1 (en) * | 2002-04-23 | 2003-11-20 | U.S. Genomics, Inc. | Compositions and methods related to two-arm nucleic acid probes |
| US7371520B2 (en) * | 2002-05-28 | 2008-05-13 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
| EP1543155A4 (en) * | 2002-07-17 | 2006-08-16 | Us Genomics Inc | METHOD AND COMPOSITIONS FOR THE ANALYSIS OF POLYMERS USING CHIMERIC TAGS |
| US20040214211A1 (en) * | 2003-01-23 | 2004-10-28 | U.S. Genomics, Inc. | Methods for analyzing polymer populations |
| US20050123944A1 (en) * | 2003-08-01 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions |
| WO2005022162A1 (en) * | 2003-08-21 | 2005-03-10 | U.S. Genomics, Inc. | Quantum dots and methods of use thereof |
| US20050142595A1 (en) * | 2003-11-07 | 2005-06-30 | U.S. Genomics, Inc. | Intercalator FRET donors or acceptors |
| EP1704256A4 (en) * | 2004-01-13 | 2008-01-16 | Us Genomics Inc | Detection and quantification of analytes in solution using polymers |
-
2003
- 2003-05-09 EP EP03811981A patent/EP1540017A4/en not_active Withdrawn
- 2003-05-09 JP JP2004555256A patent/JP2005537030A/ja not_active Withdrawn
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- 2003-05-09 AU AU2003302463A patent/AU2003302463A1/en not_active Abandoned
- 2003-05-09 US US10/435,399 patent/US20030235854A1/en not_active Abandoned
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010522569A (ja) * | 2007-03-28 | 2010-07-08 | バイオナノマトリックス、インク． | ナノチャネルアレイを用いた巨大分子解析方法 |
| JP2014097058A (ja) * | 2007-03-28 | 2014-05-29 | Bionanomatrix Llc | ナノチャネルアレイを用いた巨大分子解析方法 |
| JP2010233496A (ja) * | 2009-03-31 | 2010-10-21 | Sekisui Medical Co Ltd | Ｕｇｔ１ａ１遺伝子多型検出法 |
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| EP1540017A2 (en) | 2005-06-15 |
| WO2004048514A3 (en) | 2005-04-21 |
| AU2003302463A1 (en) | 2004-06-18 |
| WO2004048514A2 (en) | 2004-06-10 |
| US20030235854A1 (en) | 2003-12-25 |
| EP1540017A4 (en) | 2006-05-24 |
| AU2003302463A8 (en) | 2004-06-18 |
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