JP2005537030A5 - - Google Patents
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- JP2005537030A5 JP2005537030A5 JP2004555256A JP2004555256A JP2005537030A5 JP 2005537030 A5 JP2005537030 A5 JP 2005537030A5 JP 2004555256 A JP2004555256 A JP 2004555256A JP 2004555256 A JP2004555256 A JP 2004555256A JP 2005537030 A5 JP2005537030 A5 JP 2005537030A5
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- Prior art keywords
- polynucleotide
- specific marker
- unit specific
- haplotype
- labeled
- Prior art date
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- 238000000034 method Methods 0.000 claims description 59
- 108091033319 polynucleotide Proteins 0.000 claims description 46
- 102000040430 polynucleotide Human genes 0.000 claims description 46
- 239000002157 polynucleotide Substances 0.000 claims description 46
- 239000003550 marker Substances 0.000 claims description 28
- 108091034117 Oligonucleotide Proteins 0.000 claims description 24
- 102000054766 genetic haplotypes Human genes 0.000 claims description 21
- 108020004414 DNA Proteins 0.000 claims description 19
- 239000002773 nucleotide Substances 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 16
- 238000001514 detection method Methods 0.000 claims description 16
- 239000000523 sample Substances 0.000 claims description 16
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 125000003729 nucleotide group Chemical group 0.000 claims description 14
- 238000009396 hybridization Methods 0.000 claims description 12
- 238000002372 labelling Methods 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- -1 oligo beacon Proteins 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 230000002068 genetic effect Effects 0.000 claims description 4
- 239000001226 triphosphate Substances 0.000 claims description 4
- 235000011178 triphosphate Nutrition 0.000 claims description 4
- 230000005670 electromagnetic radiation Effects 0.000 claims description 3
- 238000009830 intercalation Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims description 2
- 238000000137 annealing Methods 0.000 claims 2
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- 108060002716 Exonuclease Proteins 0.000 claims 1
- 108091092878 Microsatellite Proteins 0.000 claims 1
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- 102000015636 Oligopeptides Human genes 0.000 claims 1
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- 108010010677 Phosphodiesterase I Proteins 0.000 claims 1
- 230000009471 action Effects 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000004891 communication Methods 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
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- 238000012360 testing method Methods 0.000 claims 1
- 229920000642 polymer Polymers 0.000 description 12
- 238000010586 diagram Methods 0.000 description 9
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 8
- 239000012634 fragment Substances 0.000 description 7
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- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
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- SAOKZLXYCUGLFA-UHFFFAOYSA-N bis(2-ethylhexyl) adipate Chemical compound CCCCC(CC)COC(=O)CCCCC(=O)OCC(CC)CCCC SAOKZLXYCUGLFA-UHFFFAOYSA-N 0.000 description 3
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- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
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- 229940104230 thymidine Drugs 0.000 description 2
- 102000007469 Actins Human genes 0.000 description 1
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- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
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- 102000005936 beta-Galactosidase Human genes 0.000 description 1
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- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
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- 238000004925 denaturation Methods 0.000 description 1
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- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000000138 intercalating agent Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
Images
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37946102P | 2002-05-09 | 2002-05-09 | |
| PCT/US2003/014776 WO2004048514A2 (en) | 2002-05-09 | 2003-05-09 | Methods for analyzing a nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005537030A JP2005537030A (ja) | 2005-12-08 |
| JP2005537030A5 true JP2005537030A5 (https=) | 2006-06-29 |
Family
ID=32393218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004555256A Withdrawn JP2005537030A (ja) | 2002-05-09 | 2003-05-09 | 核酸を分析する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20030235854A1 (https=) |
| EP (1) | EP1540017A4 (https=) |
| JP (1) | JP2005537030A (https=) |
| AU (1) | AU2003302463A1 (https=) |
| WO (1) | WO2004048514A2 (https=) |
Families Citing this family (88)
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| WO1998035012A2 (en) | 1997-02-12 | 1998-08-13 | Chan Eugene Y | Methods and products for analyzing polymers |
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| US20050123944A1 (en) * | 2003-08-01 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions |
| WO2005017205A2 (en) * | 2003-08-04 | 2005-02-24 | U. S. Genomics, Inc. | Nucleic acid mapping using linear analysis |
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| US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
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| US7575863B2 (en) * | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
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| US7642055B2 (en) * | 2004-09-21 | 2010-01-05 | Applied Biosystems, Llc | Two-color real-time/end-point quantitation of microRNAs (miRNAs) |
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| WO2006044728A2 (en) * | 2004-10-18 | 2006-04-27 | U.S.Genomics, Inc. | Methods for isolation of nucleic acids from prokaryotic spores |
| WO2007011660A2 (en) * | 2005-07-14 | 2007-01-25 | William Marsh Rice University | Quantum dot probes |
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| EP1543155A4 (en) * | 2002-07-17 | 2006-08-16 | Us Genomics Inc | METHOD AND COMPOSITIONS FOR THE ANALYSIS OF POLYMERS USING CHIMERIC TAGS |
| US20040214211A1 (en) * | 2003-01-23 | 2004-10-28 | U.S. Genomics, Inc. | Methods for analyzing polymer populations |
| US20050123944A1 (en) * | 2003-08-01 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions |
| WO2005022162A1 (en) * | 2003-08-21 | 2005-03-10 | U.S. Genomics, Inc. | Quantum dots and methods of use thereof |
| US20050142595A1 (en) * | 2003-11-07 | 2005-06-30 | U.S. Genomics, Inc. | Intercalator FRET donors or acceptors |
| EP1704256A4 (en) * | 2004-01-13 | 2008-01-16 | Us Genomics Inc | Detection and quantification of analytes in solution using polymers |
-
2003
- 2003-05-09 EP EP03811981A patent/EP1540017A4/en not_active Withdrawn
- 2003-05-09 JP JP2004555256A patent/JP2005537030A/ja not_active Withdrawn
- 2003-05-09 WO PCT/US2003/014776 patent/WO2004048514A2/en not_active Ceased
- 2003-05-09 AU AU2003302463A patent/AU2003302463A1/en not_active Abandoned
- 2003-05-09 US US10/435,399 patent/US20030235854A1/en not_active Abandoned
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