JP2005519145A5 - - Google Patents
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- Publication number
- JP2005519145A5 JP2005519145A5 JP2003571320A JP2003571320A JP2005519145A5 JP 2005519145 A5 JP2005519145 A5 JP 2005519145A5 JP 2003571320 A JP2003571320 A JP 2003571320A JP 2003571320 A JP2003571320 A JP 2003571320A JP 2005519145 A5 JP2005519145 A5 JP 2005519145A5
- Authority
- JP
- Japan
- Prior art keywords
- sodium
- aqueous solution
- polyacrylamide gel
- enclosed
- oxygen scavenger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920003023 plastic Polymers 0.000 claims 18
- 239000004033 plastic Substances 0.000 claims 18
- 229920002401 polyacrylamide Polymers 0.000 claims 16
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims 14
- 239000007864 aqueous solution Substances 0.000 claims 13
- 229940123973 Oxygen scavenger Drugs 0.000 claims 12
- 238000000034 method Methods 0.000 claims 11
- 239000000203 mixture Substances 0.000 claims 10
- 239000000178 monomer Substances 0.000 claims 10
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 9
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 claims 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 8
- 235000010265 sodium sulphite Nutrition 0.000 claims 7
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 6
- 239000011734 sodium Substances 0.000 claims 6
- 229910052708 sodium Inorganic materials 0.000 claims 6
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims 5
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims 5
- AOSFMYBATFLTAQ-UHFFFAOYSA-N 1-amino-3-(benzimidazol-1-yl)propan-2-ol Chemical compound C1=CC=C2N(CC(O)CN)C=NC2=C1 AOSFMYBATFLTAQ-UHFFFAOYSA-N 0.000 claims 4
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-araboascorbic acid Natural products OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 claims 4
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims 4
- 235000010350 erythorbic acid Nutrition 0.000 claims 4
- 239000004318 erythorbic acid Substances 0.000 claims 4
- 229940026239 isoascorbic acid Drugs 0.000 claims 4
- -1 polyethylene terephthalate Polymers 0.000 claims 4
- 235000015424 sodium Nutrition 0.000 claims 4
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims 4
- 235000019345 sodium thiosulphate Nutrition 0.000 claims 4
- ZYZYQCACSQDPSB-UHFFFAOYSA-N 12,15-dioxatricyclo[8.6.0.02,7]hexadeca-1(10),2,4,6,8-pentaene-11,16-dione Chemical compound O=C1OCCOC(=O)C2=C1C=CC1=CC=CC=C21 ZYZYQCACSQDPSB-UHFFFAOYSA-N 0.000 claims 2
- 229920001174 Diethylhydroxylamine Polymers 0.000 claims 2
- 239000004793 Polystyrene Substances 0.000 claims 2
- 150000003926 acrylamides Chemical class 0.000 claims 2
- 235000010323 ascorbic acid Nutrition 0.000 claims 2
- 229960005070 ascorbic acid Drugs 0.000 claims 2
- 239000011668 ascorbic acid Substances 0.000 claims 2
- 239000003431 cross linking reagent Substances 0.000 claims 2
- FVCOIAYSJZGECG-UHFFFAOYSA-N diethylhydroxylamine Chemical compound CCN(O)CC FVCOIAYSJZGECG-UHFFFAOYSA-N 0.000 claims 2
- AGGFJZYMAXECOR-UHFFFAOYSA-N hexane-3,3-diol Chemical compound CCCC(O)(O)CC AGGFJZYMAXECOR-UHFFFAOYSA-N 0.000 claims 2
- 239000003999 initiator Substances 0.000 claims 2
- WHIVNJATOVLWBW-UHFFFAOYSA-N n-butan-2-ylidenehydroxylamine Chemical compound CCC(C)=NO WHIVNJATOVLWBW-UHFFFAOYSA-N 0.000 claims 2
- 229920002285 poly(styrene-co-acrylonitrile) Polymers 0.000 claims 2
- 229920000515 polycarbonate Polymers 0.000 claims 2
- 239000004417 polycarbonate Substances 0.000 claims 2
- 229920000139 polyethylene terephthalate Polymers 0.000 claims 2
- 239000005020 polyethylene terephthalate Substances 0.000 claims 2
- 230000000379 polymerizing effect Effects 0.000 claims 2
- 229920002223 polystyrene Polymers 0.000 claims 2
- 150000003254 radicals Chemical class 0.000 claims 2
- 238000005266 casting Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/087,140 US6846881B2 (en) | 2002-02-27 | 2002-02-27 | Preparation of defect-free polyacrylamide electrophoresis gels in plastic cassettes |
| PCT/US2003/006207 WO2003072616A1 (en) | 2002-02-27 | 2003-02-26 | Preparation of defect-free polyacrylamide electrophoresis gels in plastic cassettes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005519145A JP2005519145A (ja) | 2005-06-30 |
| JP2005519145A5 true JP2005519145A5 (https=) | 2006-06-22 |
| JP4287284B2 JP4287284B2 (ja) | 2009-07-01 |
Family
ID=27753895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003571320A Expired - Fee Related JP4287284B2 (ja) | 2002-02-27 | 2003-02-26 | プラスチック製カセット中の欠陥のないポリアクリルアミド電気泳動ゲルの作製 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6846881B2 (https=) |
| EP (1) | EP1478670B1 (https=) |
| JP (1) | JP4287284B2 (https=) |
| AU (1) | AU2003212467C1 (https=) |
| CA (1) | CA2476768C (https=) |
| DE (1) | DE60322027D1 (https=) |
| WO (1) | WO2003072616A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6846881B2 (en) * | 2002-02-27 | 2005-01-25 | Bio-Rad Laboratories, Inc. | Preparation of defect-free polyacrylamide electrophoresis gels in plastic cassettes |
| EP1864123A1 (en) * | 2004-11-26 | 2007-12-12 | GE Healthcare Bio-Sciences AB | Gel composite |
| US20090211907A1 (en) * | 2005-02-25 | 2009-08-27 | Japan Science And Technology Agency | Separation Medium for Biochemical Analysis |
| ES2294949B1 (es) * | 2006-09-22 | 2009-04-01 | Universidad De Barcelona | Gel para la separacion de proteinas. |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US20080272002A1 (en) * | 2007-05-03 | 2008-11-06 | Protein Forest, Inc. | System and Method for Proteomics |
| DE102008006086A1 (de) * | 2008-01-25 | 2009-09-03 | Evonik Goldschmidt Gmbh | Biomimetische Modellsysteme zur Nachstellung von Spreitungsphänomenen kosmetischer und pharmazeutischer Formulierungen auf menschlicher Haut |
| US20100032296A1 (en) * | 2008-08-07 | 2010-02-11 | Protein Forest, Inc. | Systems and methods for quantitative analyte transfer |
| US8574835B2 (en) * | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| US8653567B2 (en) | 2010-07-03 | 2014-02-18 | Life Technologies Corporation | Chemically sensitive sensor with lightly doped drains |
| WO2014081874A1 (en) | 2012-11-20 | 2014-05-30 | Bio-Rad Laboratories, Inc. | Polyacrylamide electrophoresis gels with protection against oxygen exposure |
| US11298678B2 (en) | 2016-03-30 | 2022-04-12 | Trustees Of Tufts College | Fabrication of macroporous polymeric hydrogel microparticles |
| CN107266692A (zh) * | 2017-05-12 | 2017-10-20 | 李宗津 | 纳米复合水凝胶材料及制备方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3755280A (en) * | 1971-03-05 | 1973-08-28 | American Cyanamid Co | Drying of acrylamide polymer gels |
| US4806434A (en) * | 1984-07-06 | 1989-02-21 | Fuji Photo Film Co., Ltd. | Medium for electrophoresis |
| EP0172723B2 (en) * | 1984-08-15 | 1997-11-05 | Ciba Specialty Chemicals Water Treatments Limited | Water soluble polymers |
| US4746551A (en) * | 1985-11-20 | 1988-05-24 | Micro-Map, Inc. | Rehydratable polyacrylamide gels |
| US5292665A (en) * | 1989-03-15 | 1994-03-08 | The United States Of America As Represented By The Department Of Health And Human Services | Catalyst for preparing polyacrylamide gel which improves the detection of biomaterials by silver staining |
| US5149416A (en) * | 1989-04-18 | 1992-09-22 | Eastman Kodak Company | Polymeric electrophoresis media |
| US5290411A (en) * | 1992-07-14 | 1994-03-01 | California Institute Of Technology | Acrylic polymer electrophoresis support media |
| AU3512695A (en) * | 1994-09-19 | 1996-04-09 | Novel Experimental Technology | Plastic mold for electrophoresis gel |
| AUPO093296A0 (en) * | 1996-07-10 | 1996-08-01 | University Of Melbourne, The | Electrophoresis gels and crosslinking agents for their preparation |
| EP1032827A1 (en) * | 1997-05-13 | 2000-09-06 | Hoefer Pharmacia Biotech, Inc. | Gel casting and electrophoresis device |
| US6846881B2 (en) * | 2002-02-27 | 2005-01-25 | Bio-Rad Laboratories, Inc. | Preparation of defect-free polyacrylamide electrophoresis gels in plastic cassettes |
-
2002
- 2002-02-27 US US10/087,140 patent/US6846881B2/en not_active Expired - Lifetime
-
2003
- 2003-02-26 CA CA002476768A patent/CA2476768C/en not_active Expired - Fee Related
- 2003-02-26 DE DE60322027T patent/DE60322027D1/de not_active Expired - Lifetime
- 2003-02-26 AU AU2003212467A patent/AU2003212467C1/en not_active Ceased
- 2003-02-26 JP JP2003571320A patent/JP4287284B2/ja not_active Expired - Fee Related
- 2003-02-26 WO PCT/US2003/006207 patent/WO2003072616A1/en not_active Ceased
- 2003-02-26 EP EP03709407A patent/EP1478670B1/en not_active Expired - Lifetime
-
2004
- 2004-10-25 US US10/973,541 patent/US20050059761A1/en not_active Abandoned
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