JP2005500052A5 - - Google Patents
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- Publication number
- JP2005500052A5 JP2005500052A5 JP2003517223A JP2003517223A JP2005500052A5 JP 2005500052 A5 JP2005500052 A5 JP 2005500052A5 JP 2003517223 A JP2003517223 A JP 2003517223A JP 2003517223 A JP2003517223 A JP 2003517223A JP 2005500052 A5 JP2005500052 A5 JP 2005500052A5
- Authority
- JP
- Japan
- Prior art keywords
- polypeptide
- nucleic acid
- aaa
- aag
- fusion polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000004927 fusion Effects 0.000 claims description 47
- 210000004027 cell Anatomy 0.000 claims description 23
- 230000027455 binding Effects 0.000 claims description 16
- 239000005557 antagonist Substances 0.000 claims description 8
- 230000004071 biological effect Effects 0.000 claims description 7
- 102000004278 Receptor Protein-Tyrosine Kinases Human genes 0.000 claims description 6
- 108090000873 Receptor Protein-Tyrosine Kinases Proteins 0.000 claims description 6
- 239000000556 agonist Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 102000001301 EGF receptor Human genes 0.000 claims description 4
- 108060006698 EGF receptor Proteins 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 92
- 229920001184 polypeptide Polymers 0.000 claims 88
- 102000004196 processed proteins & peptides Human genes 0.000 claims 88
- 150000007523 nucleic acids Chemical class 0.000 claims 41
- 108020004707 nucleic acids Proteins 0.000 claims 29
- 102000039446 nucleic acids Human genes 0.000 claims 29
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims 20
- 229960002897 heparin Drugs 0.000 claims 20
- 229920000669 heparin Polymers 0.000 claims 17
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 16
- 239000003446 ligand Substances 0.000 claims 14
- 229920002971 Heparan sulfate Polymers 0.000 claims 13
- 102000005962 receptors Human genes 0.000 claims 13
- 108020003175 receptors Proteins 0.000 claims 13
- 230000008685 targeting Effects 0.000 claims 7
- 102000000844 Cell Surface Receptors Human genes 0.000 claims 6
- 108010001857 Cell Surface Receptors Proteins 0.000 claims 6
- 239000013604 expression vector Substances 0.000 claims 6
- 210000004962 mammalian cell Anatomy 0.000 claims 6
- 238000000159 protein binding assay Methods 0.000 claims 6
- 239000008194 pharmaceutical composition Substances 0.000 claims 5
- 241000282326 Felis catus Species 0.000 claims 4
- 206010028980 Neoplasm Diseases 0.000 claims 4
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 4
- 208000012902 Nervous system disease Diseases 0.000 claims 4
- 102000014413 Neuregulin Human genes 0.000 claims 4
- 108050003475 Neuregulin Proteins 0.000 claims 4
- 201000010099 disease Diseases 0.000 claims 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 4
- 101800003838 Epidermal growth factor Proteins 0.000 claims 3
- 102400001368 Epidermal growth factor Human genes 0.000 claims 3
- 102000008055 Heparan Sulfate Proteoglycans Human genes 0.000 claims 3
- 241001465754 Metazoa Species 0.000 claims 3
- 102000007072 Nerve Growth Factors Human genes 0.000 claims 3
- 108090000054 Syndecan-2 Proteins 0.000 claims 3
- 150000001413 amino acids Chemical group 0.000 claims 3
- 239000004020 conductor Substances 0.000 claims 3
- 229940116977 epidermal growth factor Drugs 0.000 claims 3
- 239000003900 neurotrophic factor Substances 0.000 claims 3
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 3
- JVJUWEFOGFCHKR-UHFFFAOYSA-N 2-(diethylamino)ethyl 1-(3,4-dimethylphenyl)cyclopentane-1-carboxylate;hydrochloride Chemical compound Cl.C=1C=C(C)C(C)=CC=1C1(C(=O)OCCN(CC)CC)CCCC1 JVJUWEFOGFCHKR-UHFFFAOYSA-N 0.000 claims 2
- 102000002110 C2 domains Human genes 0.000 claims 2
- 108050009459 C2 domains Proteins 0.000 claims 2
- 108090000695 Cytokines Proteins 0.000 claims 2
- 102000004127 Cytokines Human genes 0.000 claims 2
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims 2
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims 2
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 claims 2
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 claims 2
- 108060003951 Immunoglobulin Proteins 0.000 claims 2
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims 2
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims 2
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 claims 2
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 claims 2
- 102000010803 Netrins Human genes 0.000 claims 2
- 108010063605 Netrins Proteins 0.000 claims 2
- 108010009583 Transforming Growth Factors Proteins 0.000 claims 2
- 102000009618 Transforming Growth Factors Human genes 0.000 claims 2
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 claims 2
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 2
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 2
- 230000004913 activation Effects 0.000 claims 2
- 201000011510 cancer Diseases 0.000 claims 2
- 239000003937 drug carrier Substances 0.000 claims 2
- 230000002708 enhancing effect Effects 0.000 claims 2
- 108060002566 ephrin Proteins 0.000 claims 2
- 102000012803 ephrin Human genes 0.000 claims 2
- 229940126864 fibroblast growth factor Drugs 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 239000003102 growth factor Substances 0.000 claims 2
- 210000005260 human cell Anatomy 0.000 claims 2
- 102000018358 immunoglobulin Human genes 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 208000015122 neurodegenerative disease Diseases 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims 2
- 230000000472 traumatic effect Effects 0.000 claims 2
- 208000024827 Alzheimer disease Diseases 0.000 claims 1
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 claims 1
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 claims 1
- 102000034615 Glial cell line-derived neurotrophic factor Human genes 0.000 claims 1
- 108091010837 Glial cell line-derived neurotrophic factor Proteins 0.000 claims 1
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 1
- 108090000742 Neurotrophin 3 Proteins 0.000 claims 1
- 102000004230 Neurotrophin 3 Human genes 0.000 claims 1
- 108090000099 Neurotrophin-4 Proteins 0.000 claims 1
- 102000003683 Neurotrophin-4 Human genes 0.000 claims 1
- 208000018737 Parkinson disease Diseases 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 208000010886 Peripheral nerve injury Diseases 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 208000006011 Stroke Diseases 0.000 claims 1
- 208000030886 Traumatic Brain injury Diseases 0.000 claims 1
- 206010002026 amyotrophic lateral sclerosis Diseases 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 229940077737 brain-derived neurotrophic factor Drugs 0.000 claims 1
- 239000000539 dimer Substances 0.000 claims 1
- 206010015037 epilepsy Diseases 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 201000006417 multiple sclerosis Diseases 0.000 claims 1
- 229940053128 nerve growth factor Drugs 0.000 claims 1
- 230000004770 neurodegeneration Effects 0.000 claims 1
- 229940032018 neurotrophin 3 Drugs 0.000 claims 1
- 229940097998 neurotrophin 4 Drugs 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 238000003259 recombinant expression Methods 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 208000020431 spinal cord injury Diseases 0.000 claims 1
- 230000000638 stimulation Effects 0.000 claims 1
- 230000009529 traumatic brain injury Effects 0.000 claims 1
- 239000013638 trimer Substances 0.000 claims 1
- 239000013603 viral vector Substances 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000026731 phosphorylation Effects 0.000 description 3
- 238000006366 phosphorylation reaction Methods 0.000 description 3
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
- 101710154606 Hemagglutinin Proteins 0.000 description 1
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
- 101710176177 Protein A56 Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 239000000185 hemagglutinin Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 210000000107 myocyte Anatomy 0.000 description 1
- DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30856301P | 2001-07-31 | 2001-07-31 | |
| PCT/US2002/024053 WO2003012045A2 (en) | 2001-07-31 | 2002-07-31 | Hybrid proteins with neuregulin heparin-binding domain for targeting to heparan sulfate proteoglycans |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005500052A JP2005500052A (ja) | 2005-01-06 |
| JP2005500052A5 true JP2005500052A5 (https=) | 2006-06-15 |
| JP4314113B2 JP4314113B2 (ja) | 2009-08-12 |
Family
ID=23194471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003517223A Expired - Fee Related JP4314113B2 (ja) | 2001-07-31 | 2002-07-31 | ヘパラン硫酸プロテオグリカンに対する標的化のための、ニューレグリンヘパリン結合ドメインとのハイブリッドタンパク質 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7527794B2 (https=) |
| EP (1) | EP1456239B1 (https=) |
| JP (1) | JP4314113B2 (https=) |
| AT (1) | ATE427317T1 (https=) |
| AU (1) | AU2002322762B2 (https=) |
| CA (1) | CA2455830C (https=) |
| DE (1) | DE60231833D1 (https=) |
| WO (1) | WO2003012045A2 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1824879B1 (en) * | 2004-07-09 | 2014-10-29 | Wayne State University | HYBRID PROTEINS WITH ErbB4 EXTRACELLULAR DOMAIN AND NEUREGULIN HEPARIN-BINDING DOMAIN FOR TARGETING |
| US20110034383A1 (en) * | 2007-10-15 | 2011-02-10 | Institut Pasteur | Cxcl12 gamma a chemokine and uses thereof |
| EP2346526A2 (en) | 2008-09-22 | 2011-07-27 | Massachusetts Institute of Technology | Compositions of and methods of using ligand dimers |
| US9029328B2 (en) | 2010-03-24 | 2015-05-12 | The Brigham And Women's Hospital, Inc. | Methods for cardioprotection and cardioregeneration with dimers of EGF family ligands |
| CN103118698A (zh) * | 2010-05-28 | 2013-05-22 | 意识-Nrg公司 | 神经调节素同种型，神经调节素多肽和其使用 |
| JP2014520148A (ja) * | 2011-06-20 | 2014-08-21 | セントルイス ユニバーシティ | 治療のための神経筋接合部への標的化 |
| US20130058871A1 (en) * | 2011-07-28 | 2013-03-07 | Howard Hughes Medical Institute | Method and system for mapping synaptic connectivity using light microscopy |
| US9790264B2 (en) | 2012-06-25 | 2017-10-17 | The Brigham And Women's Hospital, Inc. | Compounds and methods for modulating pharmacokinetics |
| EP2864360B1 (en) | 2012-06-25 | 2017-09-06 | The Brigham and Women's Hospital, Inc. | Targeted therapeutics |
| EP3247380A1 (en) * | 2015-01-20 | 2017-11-29 | Universiteit Antwerpen | Neuregulin in the treatment of fibrotic disorders |
| WO2016123163A2 (en) | 2015-01-27 | 2016-08-04 | Kardiatonos, Inc. | Biomarkers of vascular disease |
| US12466865B2 (en) | 2019-01-03 | 2025-11-11 | The Board Of Trustees Of The University Of Illinois | Method and kit for treating a neurodegenerative disease |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8947791A (en) * | 1990-11-01 | 1992-05-26 | Scripps Research Institute, The | Glycosaminoglycan-targeted fusion proteins, their design, construction and compositions |
| US5367060A (en) * | 1991-05-24 | 1994-11-22 | Genentech, Inc. | Structure, production and use of heregulin |
| FI945085A0 (fi) * | 1992-04-29 | 1994-10-28 | Amgen Inc | Yhdistelmä-neu-reseptoristimulaatiotekijä |
| CA2145769A1 (en) * | 1992-09-29 | 1994-04-14 | Gerald D. Fischbach | Trophic factor having ion channel-inducing activity in neuronal cells |
| US6037329A (en) * | 1994-03-15 | 2000-03-14 | Selective Genetics, Inc. | Compositions containing nucleic acids and ligands for therapeutic treatment |
| WO1997009051A1 (en) | 1995-09-05 | 1997-03-13 | Cambridge Neuroscience, Inc. | Analogs for specific oligosaccharide-neuregulin interactions and uses thereof |
| US6344436B1 (en) * | 1996-01-08 | 2002-02-05 | Baylor College Of Medicine | Lipophilic peptides for macromolecule delivery |
| WO1998002540A1 (en) * | 1996-07-12 | 1998-01-22 | Genentech, Inc. | Chimeric heteromultimer adhesins |
| CA2257839C (en) * | 1996-07-12 | 2012-10-23 | Genentech, Inc. | Gamma-heregulin |
| KR100195886B1 (ko) | 1996-11-01 | 1999-06-15 | 김상조 | 당뇨병 치료용 의약조성물 |
| WO1998043649A2 (en) | 1997-03-28 | 1998-10-08 | Otsuka Pharmaceutical Co., Ltd. | Age production inhibitory composition comprising a maillard reaction inhibitor and vitamin b¿6? |
| AU4469997A (en) | 1997-09-17 | 1999-04-05 | Urs Eppenberger | Heregulin-gamma |
| WO1999055351A1 (en) | 1998-04-23 | 1999-11-04 | Vladimir Leko | Herbal composition and medicament against diabetes mellitus type ii manufactured thereof |
| US6344572B1 (en) | 1999-01-29 | 2002-02-05 | Kaneka Corporation | Processes for the preparation of threo-1,2-epoxy-3-amino-4-phenylbutane derivatives |
-
2002
- 2002-07-31 JP JP2003517223A patent/JP4314113B2/ja not_active Expired - Fee Related
- 2002-07-31 AT AT02756777T patent/ATE427317T1/de not_active IP Right Cessation
- 2002-07-31 AU AU2002322762A patent/AU2002322762B2/en not_active Ceased
- 2002-07-31 US US10/485,206 patent/US7527794B2/en not_active Expired - Lifetime
- 2002-07-31 CA CA2455830A patent/CA2455830C/en not_active Expired - Fee Related
- 2002-07-31 EP EP02756777A patent/EP1456239B1/en not_active Expired - Lifetime
- 2002-07-31 DE DE60231833T patent/DE60231833D1/de not_active Expired - Lifetime
- 2002-07-31 WO PCT/US2002/024053 patent/WO2003012045A2/en not_active Ceased
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