JP2005292112A - 生物学的材料の存在を検出し画像化するための方法及び装置 - Google Patents
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Abstract
【解決手段】非生体表面上の生物学的材料を検出するための方法及び装置において、種々の内因性蛍光団を励起することができる特定のエネルギーの電磁放射に各種試料を曝露し、これらの蛍光団が測定可能な蛍光を発する。生物学的材料からの内因性蛍光の蛍光信号からバックグラウンド、散乱励起光、反射励起光に起因する信号を除去し、内因性蛍光団からの信号強度が予想される領域に収めることが要求される。
【選択図】なし
Description
Claims (11)
- 生物学的材料を検出し識別する方法であって、次のa〜e、
a.200nmを超える電磁放射波長の特定の励起領域を有する少なくとも一種以上の生物学的内因性蛍光団を励起することにより、前記生物学的材料中の前記内因性蛍光団を励起して蛍光を発せしめ、
b.内因性蛍光の最大値及び最小値に関連する信号強度を検出し、
c.励起がない場合の内因性蛍光の最大値及び最小値に関連するバックグラウンド強度を検出し、
d.バックグラウンドを差引いた最小値の強度から、蛍光の最大値における反射及び散乱強度を適切なアルゴリズムを用いて計算し、
e.検出された生物学的蛍光の信号から、計算された反射及び散乱強度と計測されたバックグラウンド強度とを差引くことにより、バックグラウンド寄与分と反射寄与分と散乱寄与分とが差引かれた検出蛍光の大きさにより材料量を決定する、を含む方法。 - 請求項1に記載の方法において、測定されたバックグラウンドと計算された反射と散乱とが差引かれた複数の蛍光信号強度の比が決定され、それにより、次の条件即ち、バックグラウンド、散乱、反射がそれぞれ補正された信号の比が一定の領域内にあり、且つ信号の比が前記予想される領域内にある前記検出信号の大きさによって材料量を決定する条件に依存して前記生物学的材料の識別がなされる方法。
- 請求項1に記載の方法において、前記生物学的内因性蛍光は320〜360、380〜460、430〜480、420〜510、430〜540、480〜570、630〜700、760〜840及び860〜930nm領域の蛍光群から選択される方法。
- 生物学的材料を検出し識別する方法であって、次のa〜f、
a.200nm〜300nmの種々の励起波長の紫外電磁照射を用いて複数の生物学的内因性蛍光団を励起することにより、存在するいずれかの生物学的材料中の内因性蛍光団を励起し蛍光を発せしめ、その蛍光の一部を自己吸収して他の内因性蛍光団を励起し新たに蛍光を発せしめ、
b.内因性蛍光の最大値及び最小値に関連する蛍光信号強度を検出し、
c.励起がない場合の内因性蛍光の最大値及び最小値に関連するバックグラウンド強度を検出し、
d.バックグラウンドを差引いた最小値の強度から、蛍光の最大値における反射及び散乱強度を適切なアルゴリズムを用いて計算し、
e.検出された生物学的蛍光の信号から、計算された反射及び散乱強度と計測されたバックグラウンド強度とを差引き、
f.バックグラウンド寄与分と反射寄与分と散乱寄与分とが差引かれた検出蛍光信号の比が予想される領域にあることを決定し、比が予想される領域にあるバックグラウンド寄与分と反射寄与分と散乱寄与分とが差引かれた検出蛍光信号の大きさにより生物学的材料量を決定する、を含む方法。 - 請求項4に記載の方法において、前記生物学的内因性蛍光の生物学的内因性蛍光団は320〜360、380〜460、430〜480、420〜510、430〜540、480〜570、630〜700、760〜840及び860〜930nm領域の蛍光を発する群から選択される方法。
- 請求項4に記載の方法において、二次励起内因性蛍光は430〜480、420〜510、430〜540、480〜570、630〜700、760〜840及び860〜930nm等の領域を含む方法。
- 生物学的材料を検出し、識別し、画像化する方法であって、次のa〜e、
a.200nmを超える電磁放射波長の特定の励起領域を有する少なくとも一種以上の生物学的内因性蛍光団を励起することにより、前記生物学的材料中の前記内因性蛍光団を励起して蛍光を発せしめ、
b.対象の生物学的材料の内因性蛍光の予想される最大発光領域に関連する信号強度を検出して画像化し、
c.基体のバックグラウンドを、内因性蛍光の予想される発光波長の最長側の領域から少なくとも50nmを超える波長の周囲の光、反射励起放射及び散乱励起光で画像化し、
d.内因性蛍光発光領域の出力イメージと、内因性蛍光の予想される発光波長の最長側の領域を少なくとも50nmを越える波長の周囲の光、反射励起放射及び散乱励起光から得られる基体表面の出力イメージとを結合し、高波長の基体表面イメージ上に低波長イメージを存在させることによって生物学的材料を検出し、
e.検出された内因性蛍光の発光の比が予想される領域内に収まることを要求することにより生物学的材料試料を識別し、且つ検出蛍光の大きさによって材料量を決定する、を含む方法。 - 非生体表面上の生物学的材料を検出するための装置であって、次のa〜c、
a.試料に向けて電磁放射を照射する手段であって、当該手段は一種以上の生物学的内因性蛍光団を励起することができる放射エネルギーを放射するように構成された手段と、
b.放射又は反射及び散乱した放射を電気信号に変換することができる一種以上の電磁放射検出器であって、当該検出器は前記生物学的内因性蛍光団の蛍光発光の最大値及び最小値を検出するための320nmより長い波長の電磁放射を検出するように構成された検出器と、
c.内因性蛍光団の蛍光及び反射及び散乱励起の強度に対応する電気信号を分析して、生物学的材料の存在を決定する手段と、
を含む装置。 - 請求項8に記載の装置であって、電磁波が時変調されたパルスとして前記基体表面に照射される方法。
- 請求項8に記載の装置であって、前記電磁放射を照射する手段が前記電磁放射を時変調させる手段を含む装置。
- 請求項8に記載の装置であって、前記電磁放射を照射する手段が試料表面に順次的に電磁放射させる手段を含む装置。
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JP2007163873A (ja) * | 2005-12-14 | 2007-06-28 | Optrex Corp | 汚染物質の除去方法 |
WO2019012765A1 (ja) * | 2017-07-11 | 2019-01-17 | 浜松ホトニクス株式会社 | 試料観察装置及び試料観察方法 |
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US6914423B2 (en) | 2000-09-05 | 2005-07-05 | Cascade Microtech, Inc. | Probe station |
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- 2004-05-20 JP JP2004151028A patent/JP2005292112A/ja active Pending
- 2004-05-26 EP EP04012456A patent/EP1582860A1/en not_active Withdrawn
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- 2004-06-10 CN CNB2004100492958A patent/CN100561200C/zh not_active Expired - Fee Related
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JP2007163873A (ja) * | 2005-12-14 | 2007-06-28 | Optrex Corp | 汚染物質の除去方法 |
WO2019012765A1 (ja) * | 2017-07-11 | 2019-01-17 | 浜松ホトニクス株式会社 | 試料観察装置及び試料観察方法 |
JP7007122B2 (ja) | 2017-07-11 | 2022-01-24 | 浜松ホトニクス株式会社 | 試料観察装置及び試料観察方法 |
US11340165B2 (en) | 2017-07-11 | 2022-05-24 | Hamamatsu Photonics K.K. | Sample observation device and sample observation method |
Also Published As
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US20040197771A1 (en) | 2004-10-07 |
SG144722A1 (en) | 2008-08-28 |
CN100561200C (zh) | 2009-11-18 |
US7186990B2 (en) | 2007-03-06 |
CA2466433A1 (en) | 2005-10-02 |
EP1582860A1 (en) | 2005-10-05 |
CN1677088A (zh) | 2005-10-05 |
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