JP2005206466A - Wrinkle-smoothing skin lotion - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a wrinkle smoothing skin lotion effective for decreasing wrinkles and creases. <P>SOLUTION: The wrinkle-smoothing skin lotion develops excellent wrinkle-smoothing effect by simultaneously applying to the skin, an extract of the root of Valeriana officinalis L. and an extract of the root of Piper methysticum as a plant extract having muscle relaxing action, an extract of the bark of Magnoliaceae obovata Thunberg, an extract of the flower of Magnolia quinquepeta (Buc'hoz) Dandy, an extract of the aerial part such as stem or stalk of Uncaria rhynchophylla Miquel, and an extract of the peel or leaf of Vitis vinifera L and the root of Reynoutria japonica Houtt.var.japnica effective for increasing the collagen productivity of fibroblast cell. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

The present invention relates to Valeriana officinalis L. or biper (Mipoliaceae obovata Thunberg) bark extract or magnolia (Magnolia quinquepeta), which is a plant extract having a mild muscle relaxing action. (Buc'hoz) Dandy] Flower extract or fishing rattan (Uncaria rhynchophylla Miquel or Uncaria
One or more of the above-ground extracts such as sinensis oliver (stems and branches) and grape (Vitis vinifera L) pericarp or grape (Vitis vinifera L) leaves or itado (Reynoutria japonica Houtt) that enhance fibroblast collagen production .var.japnica) The present invention relates to an external preparation for improving wrinkles, which is effective in improving wrinkles and fine wrinkles by applying to the skin one or more root extract.

In recent years, as an aging society progresses, the role required of cosmetics has been increasing in order to beautifully grow older. However, due to internal factors such as aging, and external factors such as ultraviolet rays and active oxygen, the skin's inherent functions such as contractility, flexibility, and moisture retention deteriorate, causing various problems. To do.

Wrinkles, one of these troubles, are the decrease in the number of cells that produce the extracellular matrix of the dermis, the aging of cell functions such as the decrease in cell division rate, the decrease and degeneration of collagen fibers, the decrease of subcutaneous adipose tissue, etc. Due to the loss of elasticity.

Conventionally, skin aging has been dealt with by a composition in which substances such as collagen and hyaluronic acid that are lost due to aging are applied directly to the skin, or indirectly by combining protective substances to protect the skin from ultraviolet rays and active oxygen. Anti-aging agents were mainstream.

Moreover, as an attempt to fundamentally improve the generated wrinkles, α-hydroxy acids represented by retinoic acid and glycolic acid described in JP-T-2001-52065 and JP-A-3001856 are exemplified. is there. However, α-hydroxy acid requires a high blending amount, and retinoic acid has safety problems such as causing inflammation accompanied by swelling, and cannot be used for long-term use.

Another factor of wrinkle formation is thought to be excessive facial muscle tension. Facial skin muscles are under the control of facial nerve motor afferents, and the interlobular septum of the subcutaneous tissue contains fibers that make up the striated muscle tissue (skin muscle layer). It has been. Still further, a subpopulation of dermal fibroblasts known as myofibroblasts has contractile properties common to muscle tissue.

Considering this point, botulinum toxin has been conventionally used in the treatment of wrinkles in plastic surgery. As described in JP-T-2003-505343, botulinum toxin useful for reducing spasm has been shown to be capable of acting on muscle cramps and wrinkles of miken that are wrinkles between wrinkles. Furthermore, this botulinum toxin is conventionally used also in plastic surgery in the treatment of wrinkles. Therefore, although it is possible to act on the neuromuscular component of wrinkles pharmacologically, there is a drawback that the action is powerful and pain is caused by injection. In addition, botulinum toxin has a large molecular weight and is not expected to be absorbed from the skin, making it difficult to use it as a skin external preparation.
Special table 2001-52065 gazette Japanese Patent No. 3001856 Special table 2003-505343 gazette

The problem to be solved is to provide an external preparation for skin that is painless, has no harmful effects on the skin, can be used safely, has a high wrinkle-improving effect, and is effective in preventing aging with little deterioration.

Therefore, the present inventor has made extensive studies in view of such circumstances, and as a result, peeled and / or leaves of grapes (Vitis vinifera L) that enhance the collagen-producing ability of fibroblasts and roots of Japanese knotweed (Reynoutria japonica Houtt. Var. Japnica) Valeriana officinalis L., a plant extract that has a mild muscle relaxant activity and one or more selected from the extracts, birch (Piper methysticum) root extract, honoki (Magnoliaceae obovata Thunberg) Bark extract, magnolia [Magnolia quinquepeta (Buc'hoz) Dandy] flower extract, and fishing rattan (Uncaria rhynchophylla Miquel or Uncaria)
Sinensis Oliver) is found to have an extremely excellent wrinkle-improving effect by applying it to the skin in combination with one or more selected from above ground parts such as trunks and branches, and completes the present invention. It came to.

That is, the present invention relates to a skin external preparation for improving wrinkles characterized by comprising a plant extract that enhances the collagen-producing ability of dermal fibroblasts and a plant extract having a muscle relaxing action. is there.

In wrinkle improvement, wrinkles in the eyes are prevented from forming by relaxing expansion and contraction because the crease groove is formed in the part by repeatedly expanding and contracting the same place depending on the facial expression when laughing. Improving the ability to produce collagen, which is a component, can be expected to further improve wrinkles by imparting tension to the dermis and extending the surface of the skin.

Hereinafter, the configuration of the present invention will be described in detail. Grape (Vitis vinifera L) pericarp and / or leaf, Japanese knotweed (Reynoutria japonica Houtt. Var. Japnica) root extract is used as a plant extract that enhances the collagen production ability of dermal fibroblasts used in the present invention. . Plant extracts with muscle relaxant effects include Valeriana officinalis L. root extract, Piper methysticum root extract, Honoki (Magnoliaceae)
obovata Thunberg) Bark extract, Magnolia quinquepeta (Buc'hoz) Dandy flower extract, Uncaria
rhynchophylla Miquel or Uncaria sinensis Oliver) above-ground extracts such as trunks and branches are used.

EXAMPLES Next, although a test example and an Example are given and this invention is demonstrated still in detail, this invention is not limited by this. Further, unless otherwise specified, the blending amount is% by weight with respect to the entire skin external preparation.

Grapes (Vitis vinifera L.) belong to the Grapeaceae genus, are native to the western part of Asia, and are perennial deciduous vines that are widely cultivated as fruit trees in the temperate regions of the world. The flower blooms in early summer and forms conical inflorescences against the leaves of new branches. The berries produce raw food or wine.

Itadori (Reynoutria japonica Houtt.var.japnica) belongs to the genus Itadori and is widely distributed in Asia including Japan. It is a perennial that grows everywhere in Yamano. The flowers bloom in summer and the young stems are sour and edible. Rhizome is used for medicinal purposes and is called tiger root.

Valeriana (Valeriana officinalis L.) is a perennial herb that belongs to the genus Valeriana and is widely distributed in Europe and Asia with a temperate climate. Valeriana root is a sweet, slightly bitter, mild sedative used to relieve tension, emotional instability, irritability and insomnia. Essential oil components valerenic acid, valerenal and valeranone showed muscle relaxant action in mice.

Hippopotamus (Piper methysticum) is a Pepperaceae plant that has long been known to provide analgesic, sedative and relaxing effects. The active ingredient is said to be kawain.

Honoki (Magnoliaceae obovata Thunberg) is a deciduous tree that belongs to the genus Honoki and is widely distributed in Asia including Japan. The flowers bloom in late spring and have a high aroma. The old name Hogasiwa was due to the fact that food was served in the leaves. Even today, he enjoys the fragrance. Honoki's bark is said to be Japanese Kobak and is used as an astringent, healthy stomach, and diuretic. It is known that magnoclarin, an alkaloid in the park, shows a muscle relaxing action by blocking the connection between motor nerves and skeletal muscle.

Magnolia (Magnolia quinquepeta (Buc’hoz) Dandy) belongs to the genus Honoki, which is native to central China and is a large deciduous shrub cultivated for ornamental purposes. The flower blooms in the spring before the leaves, and is known as a pungent Chinese name. It is used as a sedative and analgesic. Spicy alkaloids act to block the neuromuscular junction.

The fishing rattan is a Rubiaceae lizard (Uncaria rhynchophylla Miquel or Uncaria sinensis Oliver), which has a blood pressure lowering effect, and the contained components hirsutine and isorhynchophylline produce a mild curre action in the peripheral nervous system.

Here, examples of plant extracts that have muscle relaxant activity include valeriana roots, birch roots, honoki bark, magnolia flowers, trunks / branches of fishing wicker baskets, etc., but also belladonna, thorn apple, henivein. It goes without saying that the same effect can be obtained even if a plant having a muscle relaxing action such as the above is used. These plant extracts are obtained by the following method. Grape skin, grape leaves, itadori root, valeriana root, birch root, cypress bark, magnolia flowers, trunks / branches of fishing wicker baskets, water, lower alcohol such as methanol and ethanol, hydrous lower alcohol or 1 , 3-butylene glycol, propylene glycol, or a low-polarity solvent such as water-containing polyhydric alcohol, butanol, ethyl acetate, and the like, and obtained by filtration.

The extraction conditions are not particularly limited, but are usually performed under conditions such as normal temperature, constant temperature heating, and heating reflux. After extraction, it can be filtered and used as it is, but if necessary, further purification treatment such as deodorization and decolorization is performed within a range that does not affect the effect.

Various methods are used to purify the extract, but it is adsorbed by activated carbon, styrene-divinylbenzene synthetic adsorbent (HP-20: manufactured by Mitsubishi Kasei) or octadecylsilane-treated silica (Chromatorex ODS: manufactured by Fuji Silysia Chemical). The method of eluting with an appropriate solvent is simple and practical, but is not particularly limited.

In the present invention, the amount of the grape skin and / or leaves and the locust root extract added to the skin external preparation for wrinkles is such that the dry weight of the active ingredient is 0.001 to 20.0% by weight based on the entire skin external preparation. Is preferable, and 0.01 to 5.0 weight% is more preferable. If the amount is less than 0.001% by weight, the effect of the present invention is not sufficient, and if it exceeds 20.0% by weight, coloring or the like occurs in the product, but this is not the case when decolorization is performed using a column or the like.

In the present invention, the blending amount of the valeriana root extract, birch root extract, honoki bark extract, magnolia flower extract, and the above-ground extract, such as the trunk / branch of a fishing wicker basket, into the external preparation for wrinkles, The dry weight of the active ingredient is preferably 0.001 to 10.0% by weight, more preferably 0.01 to 2.0% by weight, based on the whole external preparation for skin. If the amount is less than 0.001% by weight, the effect of the present invention is not sufficient. If the amount exceeds 10.0% by weight, coloring or the like occurs in the product, but this is not the case when the column is decolorized.

For example, the active ingredient according to the present invention is blended in various cosmetic bases, and various basic cosmetics such as creams, emulsions, lotions, packs, facial cleansers, foundations, lipsticks, cheeks, white powders, etc. It can be applied in a wide range to other cosmetics such as makeup hair, hair wash, hair nourishing agent, various hair cosmetics such as shampoo and rinse, soap, nail polish, eau de cologne. In addition, the various cosmetic embodiments can be applied in various forms such as solutions, emulsions, ointments, sols, gels, powders, and sprays.

First, the test method and standard used for evaluating the skin external preparation for wrinkles of the present invention will be described.

Production examples of the plant extract according to the present invention will be described.

[Manufacture of Extracts of 50% Itadori Root 1,3-Butylene Glycol]
5 L of 50% butylene glycol was added to 500 g of cuttlefish roots of Japanese knotweed root, and left at room temperature for 1 week. Seeds were separated by filtration to obtain a 50% butylene glycol extract of Knotweed root. The extract was subjected to distillation of the solvent under reduced pressure to obtain a locust root 50% butylene glycol extract.

[Production of Knotweed Root Ethanol Extract]
5 L of 99.5% ethanol was added to 500 g of cuttlefish roots and left at room temperature for 1 week. Seeds were separated by filtration to obtain a Knotweed root ethanol extract. The extract was subjected to distillation of the solvent under reduced pressure to obtain a locust root ethanol extract.

[Manufacture of grape skin 50% ethanol extract]
500 mL of 50% ethanol was added to 100 g of the peel from which the pulp had been removed from the grape fruit, and left at room temperature for 1 week. The mixture was filtered to obtain a 50% ethanol extract of grape skin. The extract was distilled off under reduced pressure to obtain a grape skin 50% ethanol extract.

[Production of grape skin 50% butylene glycol extract]
500 g of 50% butylene glycol was added to 100 g of the peel obtained by removing the flesh from the grape fruit, and left at room temperature for 1 week. Filtration was performed to obtain a grape skin 50% butylene glycol extract. The extract was distilled off under reduced pressure to obtain a grape skin 50% butylene glycol extract.

[Production of 50% ethanol extract of grape leaves]
600 g of 50% ethanol was added to 100 g of vine leaves and left at room temperature for 1 week. The mixture was filtered to obtain a 50% grape leaf ethanol extract. The extract was distilled off under reduced pressure to obtain a 50% ethanol extract of grape leaves.

[Manufacture of grape leaf ethanol extract]
600 g of 99.5% ethanol was added to 100 g of vine leaves and left at room temperature for 1 week. Filtration was performed to obtain a grape leaf ethanol extract. A grape leaf ethanol extract was obtained by distilling off the solvent from the extract under reduced pressure.

[Manufacture of Valeriana root 70% ethanol extract]
5 kg of 70% ethanol was added to 1 kg of valeriana roots and left at room temperature for 1 week. Filtration was performed to obtain a 70% ethanol extract of Valeriana root. The extract was distilled off under reduced pressure to obtain a 70% ethanol extract of valeriana root.

[Manufacture of 50% birch root extract]
600 g of 50% ethanol was added to 100 g of shredded birch roots and allowed to stand at room temperature for 1 week. By filtration, a 50% ethanol extract of birch root was obtained. The extract was distilled off under reduced pressure to give a 50% birch root ethanol extract.

[Production of 50% ethanol extract of honoki bark]
600 g of 50% ethanol was added to 100 g of honoki bark, and left at room temperature for 1 week. The honoki bark 50% ethanol extract was obtained by filtration. The extract was distilled off under reduced pressure to obtain 50% ethanol extract of honoki bark.

[Manufacture of magnolia petal water extract]
1000 g of water was added to 100 g of magnolia petals and extracted at 60 ° C. for 3 hours. After cooling, the mixture was filtered to obtain a magnolia petal water extract. A magnolia petal water extract was obtained by evaporating the solvent of the extract under reduced pressure.

[Manufacture of 50% ethanol extract from fishing rattan trunks]
1000 g of 50% ethanol was added to 100 g of a fish rattan carp cut and left at room temperature for 1 week. Filtration was performed to obtain a 50% ethanol extract from a fishing rattan trunk. The extract was distilled off under reduced pressure to obtain a 50% ethanol extract from a fishing wicker trunk.

[Collagen-producing effect of fibroblasts on grape skin and / or leaves and roots of Knotweed]
Fibroblasts (HFSKF-II) were seeded at 1.0 × 10 ⁵ in a 24-well chalet and cultured until confluent in Ham's F-12 medium containing 15% FBS. Various samples were added to Ham's F-12 containing no FBS and cultured for 48 hours. The synthesized collagen was quantified with an ELISA kit for measuring human type I collagen (Chondrex, Inc).

[Collagen production by fibroblasts]

As shown in Table 1, it was found that there is a function of promoting the collagen synthesis ability of fibroblasts of grape skin, grape leaves, and locust root extract.

[Use wrinkle improvement test for humans]
For 120 men and women worried about wrinkles (10 people per group), after thoroughly washing the face with face wash, apply the skin external preparations shown in Table 2 to each face once a day. Four weeks later, wrinkles were visually evaluated and replicas were collected and observed.

Degree of improvement Compared to before use, the symptom was improved by the drug used, and the usefulness was evaluated by dividing it into two stages when it was unchanged or worsened.

[Evaluation of usefulness]
A: The improvement degree is 7 or more out of 10 persons.
○: The degree of improvement is 5 to 6 among 10 people.
(Triangle | delta): The improvement of a symptom is 3-4 persons in 10 persons by the improvement degree.
X: The improvement of the symptom is 2 or less among 10 people in the degree of improvement.

Examples 1-6, Comparative Examples 1-6, lotion-type skin external preparations having the formulations shown in Table 1 were prepared, and their usefulness was evaluated by the above-described method. The results are also shown in Table 2.

As shown in Table 2, the skin external preparation of the present invention, which uses a plant extract that enhances the collagen production ability of dermal fibroblasts and a plant extract having a muscle relaxing action, is more wrinkled than the lotion of each comparative example. Excellent improvement effect.

Examples of the external preparations for improving wrinkles according to the present invention in various dosage forms are shown below. The external preparations for skin of each example were produced by a conventional method according to the specific dosage forms. Moreover, when said test and evaluation were given to the external preparation obtained in each Example, all said usefulness evaluation was "(double-circle)".

Next, the specific skin external preparation using the plant extract which concerns on this invention is demonstrated.

The formulation example of this invention is given to the following.
<Prescription Example 1> Lotion

(weight%)
Itadori Root Ethanol Extract
0.01
Valeriana root 70% ethanol extract 0.1
Polyoxyethylene sorbitan monolaurate (20E.0.) 1.5
ethanol
10.0
Preservative / Antioxidant Appropriate amount Fragrance
Suitable amount Purified water
The rest

<Prescription Example 2> Cosmetic Cream

(weight%)
Grape peel 50% ethanol extract 20.0
Honoki bark 50% ethanol extract 2.0
Beeswax
2.0
Stearyl alcohol
5.0
stearic acid
8.0
Squalane
10.0
Self-emulsifying glyceryl monostearate
3.0
Polyoxyethylene cetyl ether (20E.0.)
1.0
Propylene glycol
5.0
Potassium hydroxide
0.3
Fragrance
Suitable amount Preservative / Antioxidant
Suitable amount Purified water
The rest

<Prescription Example 3> Emulsion

(weight%)
Grape leaf ethanol extract 5.0
Fishing wicker trunk 50% ethanol extract 0.01
Squalane
8.0
Vaseline
2.0
Beeswax
0.5
Sorbitan sesquioleate
0.8
Polyoxyethylene oleyl ether (20E.0)
1.2
Carboxyvinyl polymer
0.2
Propylene glycol
0.5
Potassium hydroxide
0.1
ethanol
7.0
Perfume Appropriate amount Preservative / Antioxidant
Suitable amount Purified water
The rest

<Prescription Example 4> Packing agent

(weight%)
Knotweed Root Ethanol Extract 0.001
Magnolia flower water extract 10.0
Vinyl acetate resin emulsion
15.0
Polyvinyl alcohol
10.0
Jojoba oil
3.0
Glycerin
5.0
Titanium oxide
8.0
Kaolin
7.0
ethanol
5.0
Fragrance
Appropriate amount Preservative / Antioxidant Appropriate amount Purified water
The rest

<Prescription Example 5> Ointment

(weight%)
Grape leaf 50% ethanol extract
0.1
Valeriana root 70% ethanol extract 0.001
Octyl paradimethylaminobenzoate 4.0
Butylmethoxybenzoylmethane
4.0
Stearyl alcohol
18.0
Owl
20.0
Glycerin monostearate
0.3
Vaseline
33.0
Fragrance
Suitable amount Preservative / Antioxidant
Suitable amount Purified water
The rest

As described above, according to the present invention, it is possible to provide an external preparation for skin having an excellent wrinkle and fine wrinkle improving effect.

Claims (3)

A skin external preparation for wrinkle improvement, comprising a plant extract that enhances collagen-producing ability of skin fibroblasts and a plant extract having a muscle relaxing action. One or two plant extracts that enhance the collagen-producing ability of dermal fibroblasts are selected from grape (Vitis vinifera L) pericarp and / or leaves, and Japanese knotweed (Reynoutria japonica Houtt. Var. Japnica) root extract The skin external preparation for wrinkle improvement according to claim 1, wherein the skin external preparation is one or more species. Plant extracts with muscle relaxant effects include Valeriana officinalis L. root extract, Hippo birch (Piper methysticum) root extract, Magnoliaceae obovata Thunberg bark extract, magnolia (Magnolia quinquepeta (Buc'hoz) Dandy) ] Flower extract and fishing rattan (Uncaria rhynchophylla Miquel or Uncaria
The skin external preparation for wrinkle improvement according to claim 1, which is one or more selected from above-ground extracts such as trunks and branches of sinensis oliver).