JP2005062027A - 比色分析方法およびそれに用いる試験片 - Google Patents
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- JP2005062027A JP2005062027A JP2003293527A JP2003293527A JP2005062027A JP 2005062027 A JP2005062027 A JP 2005062027A JP 2003293527 A JP2003293527 A JP 2003293527A JP 2003293527 A JP2003293527 A JP 2003293527A JP 2005062027 A JP2005062027 A JP 2005062027A
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 14
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
【解決手段】 エレクトロクロミック物質およびグルコースデヒドロゲナーゼを含む試薬と、血液等の試料とを混合して反応させ、前記エレクトロクロミック物質の発色を測定する。前記酵素反応により遊離した電子がエレクトロクロミック物質に伝達され、グルコース濃度に応じてエレクトロクロミック物質が透明から赤紫色に発色するため、これを測定することによって、グルコース濃度を定量できる。前記エレクトロクロミック物質としては、例えば、ビオロゲン、ビオロゲン誘導体が使用できる。この反応は、過酸化水素を必要としないため、測定値の信頼性も高い。
【選択図】 図1
Description
終濃度
200mM PIPES(pH6.5) 100mM
0.1% CHAPS・2H2O 0.05%
1mM CaCl2・2H2O 0.5mM
0.1% ウシ血清アルブミン 0.05%
200 U/ml PQQ-GDH 100U/ml
(試薬B)
終濃度
4mM ビオロゲン誘導体水溶液 2mM
これらの結果を図1および図2に示す。両図は、測定波長400〜900nmにおける、反応前後の反応液の吸光度を示すグラフであり、図1は、エレクトロクロミック物質としてジニトロフェニルビオロゲンを使用した結果であり、図2は、エレクトロクロミック物質としてジフェニルビオロゲンを使用した結果である。
Claims (12)
- エレクトロクロミック物質の存在下で、分析対象物に酸化還元酵素を作用させ、前記酵素反応により前記分析対象物から遊離した電子が前記エレクトロクロミック物質に伝達される結果生じる前記エレクトロクロミック物質の色調変化を測定することにより、前記分析対象物の定性もしくは定量を行う比色分析方法。
- 前記エレクトロクロミック物質が、ビオロゲンおよびビオロゲン誘導体の少なくとも一方である請求項1記載の比色分析方法。
- ビオロゲン誘導体が、ジフェニルビオロゲンおよびジニトロフェニルビオロゲンの少なくとも一方である請求項2記載の比色分析方法。
- 酸化還元酵素が、脱水素酵素若しくは酸化酵素である請求項1〜3のいずれか一項に記載の比色分析方法。
- 分析対象物が、グルコース、コレステロール、尿酸または乳酸であり、酸化還元酵素が、前記各物質に対応する脱水素酵素または酸化酵素である請求項1〜4のいずれか一項に記載の比色分析方法。
- 請求項1〜5のいずれか一項に記載の比色分析方法に使用する試薬であって、酸化還元酵素および電子の授受により色調が変化するエレクトロクロミック物質を含む試薬。
- 前記エレクトロクロミック物質が、ビオロゲンおよびビオロゲン誘導体の少なくとも一方である請求項6記載の試薬。
- ビオロゲン誘導体が、ジフェニルビオロゲンおよびジニトロフェニルビオロゲンの少なくとも一方である請求項6記載の試薬。
- 酸化還元酵素が、脱水素酵素または酸化酵素である請求項6〜8のいずれか一項に記載の試薬。
- 分析対象物が、グルコース、コレステロール、尿酸または乳酸であり、酸化還元酵素が、前記各物質に対応する脱水素酵素若しくは酸化酵素である請求項6〜9のいずれか一項に記載の試薬。
- 請求項6〜10のいずれか一項に記載の試薬を含む試験片。
- さらに、無機ゲルを含む請求項11記載の試験片。
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2009516168A (ja) * | 2005-11-14 | 2009-04-16 | バイエル・ヘルスケア・エルエルシー | セルロースポリマーを有する試験センサ試薬 |
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JP2009516168A (ja) * | 2005-11-14 | 2009-04-16 | バイエル・ヘルスケア・エルエルシー | セルロースポリマーを有する試験センサ試薬 |
US8940153B2 (en) | 2005-11-14 | 2015-01-27 | Bayer Healthcare Llc | Test sensor reagent having cellulose polymers |
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