JP2004536565A5 - - Google Patents
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- JP2004536565A5 JP2004536565A5 JP2002559667A JP2002559667A JP2004536565A5 JP 2004536565 A5 JP2004536565 A5 JP 2004536565A5 JP 2002559667 A JP2002559667 A JP 2002559667A JP 2002559667 A JP2002559667 A JP 2002559667A JP 2004536565 A5 JP2004536565 A5 JP 2004536565A5
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| US7786261B2 (en) * | 2004-09-02 | 2010-08-31 | National Research Council Of Canada | Coiled-coil fusion proteins comprising cell receptor domains |
| JP4868731B2 (ja) * | 2004-11-17 | 2012-02-01 | 独立行政法人理化学研究所 | 哺乳動物培養細胞由来の無細胞タンパク質合成システム |
| JP4686682B2 (ja) * | 2004-12-02 | 2011-05-25 | 国立大学法人名古屋大学 | リガンド親和性複合タンパク質の取得方法、抗体スクリーニング方法、抗体スクリーニング用キット、医療方法、抗体医薬、非免疫反応性抗体医薬、投薬方法 |
| WO2006062200A1 (ja) * | 2004-12-09 | 2006-06-15 | Universal Bio Research Co., Ltd. | 核酸ライブラリー及びタンパク質ライブラリー |
| US20060234265A1 (en) * | 2005-03-21 | 2006-10-19 | Jim Richey | Microarrays having multi-functional, compartmentalized analysis areas and methods of use |
| EP1869219A4 (en) * | 2005-03-31 | 2010-06-23 | Perkinelmer Las Inc | MULTIPLEX TEST SYSTEM |
| WO2006124644A2 (en) * | 2005-05-12 | 2006-11-23 | Board Of Regents, The University Of Texas System | Protein and antibody profiling using small molecule microarrays |
| JP2006337153A (ja) * | 2005-06-01 | 2006-12-14 | Toppan Printing Co Ltd | Ｄｎａ合成判定装置、核酸タンク装置、ｄｎａチップ装置及びｄｎａ判定システム |
| GB0511717D0 (en) | 2005-06-09 | 2005-07-13 | Babraham Inst | Repeatable protein arrays |
| EP2476761A3 (en) | 2005-07-07 | 2012-10-17 | Athlomics Pty Ltd | Polynucleotide marker genes and their expression, for diagnosis of endotoxemia |
| US7889347B2 (en) | 2005-11-21 | 2011-02-15 | Plexera Llc | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
| US7463358B2 (en) * | 2005-12-06 | 2008-12-09 | Lumera Corporation | Highly stable surface plasmon resonance plates, microarrays, and methods |
| EP1989548A2 (en) * | 2006-02-08 | 2008-11-12 | Promega Corporation | Compositions and methods for capturing and analyzing cross-linked biomolecules |
| US8178316B2 (en) * | 2006-06-29 | 2012-05-15 | President And Fellows Of Harvard College | Evaluating proteins |
| SG175602A1 (en) | 2006-07-05 | 2011-11-28 | Catalyst Biosciences Inc | Protease screening methods and proteases identified thereby |
| EP2087107A2 (en) | 2006-10-30 | 2009-08-12 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
| CA2683443A1 (en) | 2007-04-13 | 2008-10-23 | Catalyst Biosciences, Inc. | Modified factor vii polypeptides and uses thereof |
| EP2210104A4 (en) * | 2007-11-01 | 2011-02-23 | Univ Arizona State | CELL-FREE PROCEDURE FOR DETECTING PROTEIN-LIGAND INTERACTIONS |
| WO2009108170A2 (en) * | 2007-11-16 | 2009-09-03 | Invitrogen Corporation | Compositions and methods for determining immune status |
| US8309035B2 (en) * | 2008-04-05 | 2012-11-13 | Single Cell Technology, Inc. | Multi-well system |
| US20100093563A1 (en) * | 2008-09-22 | 2010-04-15 | Robert Anthony Williamson | Methods and vectors for display of molecules and displayed molecules and collections |
| WO2010033237A2 (en) * | 2008-09-22 | 2010-03-25 | Calmune Corporation | Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules |
| DE102009012169B3 (de) * | 2009-03-06 | 2010-11-04 | Albert-Ludwigs-Universität Freiburg | Vorrichtung und Verfahren zum Herstellen eines Replikats oder eines Derivats aus einem Array von Molekülen und Anwendungen derselben |
| US9085798B2 (en) | 2009-04-30 | 2015-07-21 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| EP2435827A1 (en) * | 2009-05-29 | 2012-04-04 | The Board of Regents of The University of Texas System | Peptoid ligands for isolation and treatment of autoimmune t-cells |
| CA2764153C (en) * | 2009-06-02 | 2021-07-27 | The Board Of Regents Of The University Of Texas System | Identification of small molecules recognized by antibodies in subjects with neurodegenerative diseases |
| WO2011035205A2 (en) | 2009-09-18 | 2011-03-24 | Calmune Corporation | Antibodies against candida, collections thereof and methods of use |
| US8759259B2 (en) * | 2009-10-16 | 2014-06-24 | The Board Of Regents Of The University Of Texas System | Compositions and methods for producing cyclic peptoid libraries |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| WO2011127099A1 (en) | 2010-04-05 | 2011-10-13 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| WO2011139806A2 (en) * | 2010-04-27 | 2011-11-10 | Diomics Corporation | Integrated methods and systems for processing a molecular profile |
| DE102011010307A1 (de) | 2011-02-03 | 2012-08-09 | Albert-Ludwigs-Universität Freiburg | Vorrichtung und Verfahren zur Erzeugung von Protein-Mikroarrays |
| EP2694709B1 (en) | 2011-04-08 | 2016-09-14 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| JP6041154B2 (ja) * | 2011-08-12 | 2016-12-07 | 国立大学法人 筑波大学 | 並列反応方法およびスクリーニング方法 |
| DE102011054101A1 (de) | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen |
| EP4230288A3 (en) * | 2011-10-25 | 2023-11-15 | Arizona Board of Regents, a Body Corporate of the State of Arizona acting for and on behalf of Arizona State University | Programmable arrays |
| US8987174B2 (en) | 2011-10-28 | 2015-03-24 | Prognosys Biosciences, Inc. | Methods for manufacturing molecular arrays |
| WO2013090364A1 (en) | 2011-12-14 | 2013-06-20 | Arizona Board Of Regents | Method and apparatus for measuring phosphorylation kinetics on large arrays |
| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CN104903727B (zh) | 2012-11-08 | 2019-10-22 | 马克法兰伯尼特医学研究与公共健康协会有限公司 | 诊断、预后、治疗和筛选方案 |
| EP3736573A1 (en) | 2013-03-15 | 2020-11-11 | Prognosys Biosciences, Inc. | Methods for detecting peptide/mhc/tcr binding |
| US9938523B2 (en) * | 2013-03-15 | 2018-04-10 | Arizona Board Of Regents On Behalf Of Arizona State University | Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling |
| EP4036579B1 (en) | 2013-03-15 | 2025-05-07 | Arizona Board of Regents on behalf of Arizona State University | Biosensor microarray compositions and methods |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| US10288608B2 (en) | 2013-11-08 | 2019-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
| US10351842B2 (en) * | 2014-05-15 | 2019-07-16 | Arizona Board Of Regents On Behalf Of Arizona State University | Nucleic acid-guided ordered protein assemblies and methods |
| US20160195546A1 (en) | 2015-01-07 | 2016-07-07 | Arizona Board Of Regents On Behalf Of Arizona State University | Type 1 Diabetes Biomarkers |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| DK3356558T3 (da) | 2015-09-30 | 2022-04-25 | Immunexpress Pty Ltd | Sirs patogenbiomarkører og anvendelser deraf |
| EP3998345A1 (en) | 2015-12-24 | 2022-05-18 | ImmuneXpress Pty Ltd | Triage biomarkers and uses therefor |
| EP3452591B1 (en) | 2016-05-02 | 2023-08-16 | Encodia, Inc. | Macromolecule analysis employing nucleic acid encoding |
| US10055620B2 (en) * | 2017-01-13 | 2018-08-21 | International Business Machines Corporation | Baggage handling |
| US10648978B2 (en) | 2017-02-09 | 2020-05-12 | Mayo Foundation For Medical Education And Research | Methods for detecting novel autoantibodies in Crohn's disease |
| EP3698136A4 (en) * | 2017-10-16 | 2021-06-23 | Inanobio, Inc. | DISEASE PROTEAM PROTEIN ARRAYS AND USES THEREOF |
| JP7390027B2 (ja) | 2017-10-31 | 2023-12-01 | エンコディア， インコーポレイテッド | 核酸エンコーディングおよび／または標識を使用する解析のためのキット |
| SG11202003923PA (en) | 2017-10-31 | 2020-05-28 | Encodia Inc | Methods and kits using nucleic acid encoding and/or label |
| AU2019264996A1 (en) | 2018-05-10 | 2020-11-26 | The Methodist Hospital | Methods for prognosis and management of disease |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| WO2021091611A1 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| ES2982420T3 (es) | 2019-12-23 | 2024-10-16 | 10X Genomics Inc | Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
| US12399123B1 (en) | 2020-02-14 | 2025-08-26 | 10X Genomics, Inc. | Spatial targeting of analytes |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| AU2021283174A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Nucleic acid library methods |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| EP4600376A3 (en) | 2020-06-02 | 2025-10-22 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| US12435363B1 (en) | 2020-06-10 | 2025-10-07 | 10X Genomics, Inc. | Materials and methods for spatial transcriptomics |
| EP4165207B1 (en) | 2020-06-10 | 2024-09-25 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| ES2994976T3 (en) | 2020-06-25 | 2025-02-05 | 10X Genomics Inc | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US12553898B1 (en) | 2020-08-10 | 2026-02-17 | 10X Genomics, Inc. | Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| WO2022061150A2 (en) | 2020-09-18 | 2022-03-24 | 10X Geonomics, Inc. | Sample handling apparatus and image registration methods |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4421491A3 (en) | 2021-02-19 | 2024-11-27 | 10X Genomics, Inc. | Method of using a modular assay support device |
| AU2022238446A1 (en) | 2021-03-18 | 2023-09-07 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| EP4482979B1 (en) | 2022-11-09 | 2025-10-01 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
Family Cites Families (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2028165A (en) | 1978-08-17 | 1980-03-05 | Pilkington Brothers Ltd | Treatment of flat glass |
| NL8102178A (nl) * | 1981-05-02 | 1982-12-01 | Stichting Centraal Lab | Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze. |
| SE8801070D0 (sv) | 1988-03-23 | 1988-03-23 | Pharmacia Ab | Method for immobilizing a dna sequence on a solid support |
| US4868311A (en) | 1988-04-02 | 1989-09-19 | The Trustees Of Columbia University In The City Of New York | Biotinylated psoralens |
| AU649217B2 (en) * | 1988-11-18 | 1994-05-19 | Regents Of The University Of California, The | Method for site-specifically incorporating unnatural amino acids into proteins |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US6919211B1 (en) | 1989-06-07 | 2005-07-19 | Affymetrix, Inc. | Polypeptide arrays |
| US6346413B1 (en) | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
| US5874564A (en) | 1990-03-21 | 1999-02-23 | Isis Pharmaceuticals, Inc. | Reagents and methods for modulating gene expression through RNA mimicry |
| US5283179A (en) | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| US6399296B1 (en) * | 1994-07-20 | 2002-06-04 | The General Hospital Corporation | Interaction trap systems for detecting protein interactions |
| US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| EP2322614A1 (en) | 1995-06-07 | 2011-05-18 | Life Technologies Corporation | Recombinational cloning using engineered recombination sites |
| US5691152A (en) | 1995-11-09 | 1997-11-25 | E. R. Squibb & Sons, Inc. | Stable avidin composition |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| JPH1025299A (ja) | 1996-07-12 | 1998-01-27 | Nec Corp | 整列ペプチド、及びそれを用いたタンパク質の結合・相互作用部位検出方法 |
| SE9604108D0 (sv) * | 1996-11-08 | 1996-11-08 | Maans Ehrenberg | A method for the labelling of polypeptides and novel labelled polypeptides |
| ATE332368T1 (de) | 1997-01-21 | 2006-07-15 | Gen Hospital Corp | Selektion von proteinen mittels rns-protein fusionen |
| CA2338865A1 (en) | 1997-07-29 | 1999-02-11 | James S. Huston | Methods for detecting binding events using multiple binding partners and muliple targets |
| US6207370B1 (en) * | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
| CA2302147C (en) * | 1997-09-03 | 2008-05-13 | Biovation Limited | Methods for protein screening |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6303323B1 (en) | 1997-10-21 | 2001-10-16 | Cancer Research Campaign Technology Limited | Detection of dysplastic or neoplastic cells using anti-MCM5 antibodies |
| US5922617A (en) * | 1997-11-12 | 1999-07-13 | Functional Genetics, Inc. | Rapid screening assay methods and devices |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| CA2323638A1 (en) | 1998-04-03 | 1999-10-14 | Phylos, Inc. | Addressable protein arrays |
| US6780582B1 (en) | 1998-07-14 | 2004-08-24 | Zyomyx, Inc. | Arrays of protein-capture agents and methods of use thereof |
| US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
| DE59805844D1 (de) * | 1998-12-28 | 2002-11-07 | Mohr Adolf Maschf | Planschneidmaschine zum Schneiden von gestapeltem, blattförmigem Gut |
| US6251601B1 (en) | 1999-02-02 | 2001-06-26 | Vysis, Inc. | Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays |
| US6225061B1 (en) | 1999-03-10 | 2001-05-01 | Sequenom, Inc. | Systems and methods for performing reactions in an unsealed environment |
| CA2365431A1 (en) | 1999-03-10 | 2000-09-14 | Hui Ge | Universal protein array system |
| GB9917027D0 (en) | 1999-07-20 | 1999-09-22 | Affibody Technology Sweeden Ab | In vitro selection and optional identification of polypeptides using solid support carriers |
| GB9928787D0 (en) | 1999-12-03 | 2000-02-02 | Medical Res Council | Direct screening method |
| IL150291A0 (en) | 2000-01-11 | 2002-12-01 | Maxygen Inc | Integrated systems and methods for diversity generation and screening |
| KR20020064140A (ko) | 2000-01-31 | 2002-08-07 | 센스 프로테오믹 리미티드 | 단백질 발현 어레이의 제작 방법 |
| US7816098B2 (en) | 2000-01-31 | 2010-10-19 | Sense Proteomic Limited | Methods of making and using a protein array |
| AU2001255436A1 (en) | 2000-04-17 | 2001-10-30 | Transtech Pharma | Protein expression system arrays and use in biological screening |
| AU2001259512B2 (en) | 2000-05-04 | 2007-03-01 | Yale University | High density protein arrays for screening of protein activity |
| WO2001098458A2 (en) | 2000-06-19 | 2001-12-27 | Zyomyx, Inc. | Methods for immobilizing polypeptides |
| JP2004501379A (ja) | 2000-06-23 | 2004-01-15 | ドマンティス リミテッド | マトリクススクリーニング方法 |
| US6706474B1 (en) * | 2000-06-27 | 2004-03-16 | Board Of Trustees Of The University Of Illinois | Nucleic acid enzyme biosensors for ions |
| JP2004506201A (ja) | 2000-08-03 | 2004-02-26 | マサチューセッツ・インスティチュート・オブ・テクノロジー | 機能性生体分子のマイクロアレイおよびその使用 |
| CA2419490C (en) | 2000-08-15 | 2010-01-26 | Discerna Limited | Functional protein arrays |
| WO2002018648A2 (en) | 2000-08-25 | 2002-03-07 | President And Fellows Of Harvard College | Analysis of binding interactions |
| US6420150B1 (en) * | 2000-11-27 | 2002-07-16 | Pe Corporation (Ny) | Isolated human drug-metabolizing proteins, nucleic acid molecules encoding human drug-metabolizing proteins, and uses thereof |
| US6562576B2 (en) * | 2001-01-04 | 2003-05-13 | Myriad Genetics, Inc. | Yeast two-hybrid system and use thereof |
| US6800453B2 (en) | 2001-01-23 | 2004-10-05 | President And Fellows Of Harvard College | Nucleic-acid programmable protein arrays |
| US20020168640A1 (en) | 2001-02-22 | 2002-11-14 | Min Li | Biochips comprising nucleic acid/protein conjugates |
| DK1412725T3 (en) | 2001-06-29 | 2019-03-25 | Meso Scale Technologies Llc | Multi-well plates for LUMINESCENSE TEST MEASUREMENTS |
| US6929944B2 (en) | 2001-08-31 | 2005-08-16 | Beckman Coulter, Inc. | Analysis using a distributed sample |
| US20030148335A1 (en) | 2001-10-10 | 2003-08-07 | Li Shen | Detecting targets by unique identifier nucleotide tags |
| AU2003273530A1 (en) | 2002-06-03 | 2003-12-19 | Pamgene B.V. | Biomolecular kinetics method using a flow-through microarray |
| US20040127489A1 (en) | 2002-07-29 | 2004-07-01 | David Pickar | Novel antipsychotic combination therapies and compositions useful therein |
| US20060189599A1 (en) | 2002-10-18 | 2006-08-24 | Green Alan I | Treating alcohol and or substance abuse by antagonizing alpha 2 adrenergic receptors with weak dopamine blocking |
| US20040076961A1 (en) | 2002-10-21 | 2004-04-22 | Lewis Mark A. | Biomolecule retaining material and methods for attaching biomolecules to a surface |
| US7276283B2 (en) | 2004-03-24 | 2007-10-02 | Wisconsin Alumni Research Foundation | Plasma-enhanced functionalization of carbon-containing substrates |
| AU2005241003B2 (en) | 2004-04-14 | 2011-05-19 | President And Fellows Of Harvard College | Nucleic-acid programmable protein arrays |
| US20060099704A1 (en) | 2004-07-14 | 2006-05-11 | Predki Paul F | Method for providing protein microarrays |
| US20060207467A1 (en) * | 2005-03-19 | 2006-09-21 | Bailey Samuel W | Low voltage pyrotechnic igniter assembly |
| GB0511717D0 (en) | 2005-06-09 | 2005-07-13 | Babraham Inst | Repeatable protein arrays |
| US8178316B2 (en) | 2006-06-29 | 2012-05-15 | President And Fellows Of Harvard College | Evaluating proteins |
-
2002
- 2002-01-22 US US10/055,432 patent/US6800453B2/en not_active Expired - Lifetime
- 2002-01-22 JP JP2002559667A patent/JP4880188B2/ja not_active Expired - Lifetime
- 2002-01-22 EP EP02707542A patent/EP1360490B1/en not_active Expired - Lifetime
- 2002-01-22 AT AT02707542T patent/ATE538380T1/de active
- 2002-01-22 WO PCT/US2002/001882 patent/WO2002059601A1/en not_active Ceased
- 2002-01-22 CA CA2434139A patent/CA2434139C/en not_active Expired - Fee Related
-
2004
- 2004-08-03 US US10/910,718 patent/US8609344B2/en not_active Expired - Fee Related
-
2009
- 2009-01-05 JP JP2009000176A patent/JP2009150897A/ja active Pending
-
2010
- 2010-01-15 JP JP2010006896A patent/JP2010119392A/ja not_active Withdrawn
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