JP2004535189A5 - - Google Patents
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- Publication number
- JP2004535189A5 JP2004535189A5 JP2003502199A JP2003502199A JP2004535189A5 JP 2004535189 A5 JP2004535189 A5 JP 2004535189A5 JP 2003502199 A JP2003502199 A JP 2003502199A JP 2003502199 A JP2003502199 A JP 2003502199A JP 2004535189 A5 JP2004535189 A5 JP 2004535189A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant protein
- host cell
- expression
- level
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920001184 polypeptide Polymers 0.000 claims 15
- 102000004196 processed proteins & peptides Human genes 0.000 claims 15
- 108090000765 processed proteins & peptides Proteins 0.000 claims 15
- 239000000203 mixture Substances 0.000 claims 13
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 12
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 12
- 238000000034 method Methods 0.000 claims 11
- 230000014509 gene expression Effects 0.000 claims 9
- 239000013598 vector Substances 0.000 claims 8
- 230000035897 transcription Effects 0.000 claims 6
- 238000013518 transcription Methods 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 230000013595 glycosylation Effects 0.000 claims 2
- 238000006206 glycosylation reaction Methods 0.000 claims 2
- 239000012679 serum free medium Substances 0.000 claims 2
- 101000756632 Homo sapiens Actin, cytoplasmic 1 Proteins 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000011031 large-scale manufacturing process Methods 0.000 claims 1
- 230000002018 overexpression Effects 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 238000004114 suspension culture Methods 0.000 claims 1
- 230000002459 sustained effect Effects 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29596101P | 2001-06-04 | 2001-06-04 | |
| US33362001P | 2001-11-26 | 2001-11-26 | |
| US35240402P | 2002-01-29 | 2002-01-29 | |
| PCT/US2002/017763 WO2002099089A1 (en) | 2001-06-04 | 2002-06-04 | Compositions and methods for high-level, large-scale production of recombinant proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004535189A JP2004535189A (ja) | 2004-11-25 |
| JP2004535189A5 true JP2004535189A5 (https=) | 2006-01-05 |
Family
ID=27404392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003502199A Pending JP2004535189A (ja) | 2001-06-04 | 2002-06-04 | 組換えタンパク質の高レベルの大規模生成のための組成物および方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20040161817A1 (https=) |
| EP (1) | EP1402006A4 (https=) |
| JP (1) | JP2004535189A (https=) |
| KR (1) | KR20040032105A (https=) |
| CN (1) | CN1533432A (https=) |
| AU (1) | AU2002310321A1 (https=) |
| CA (1) | CA2463310A1 (https=) |
| WO (2) | WO2002099089A1 (https=) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7812148B2 (en) | 2001-04-05 | 2010-10-12 | Millipore Corporation | Vectors comprising CpG islands without position effect varigation and having increased expression |
| ATE354645T1 (de) | 2002-12-20 | 2007-03-15 | Chromagenics Bv | Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können |
| US9057079B2 (en) | 2003-02-01 | 2015-06-16 | Emd Millipore Corporation | Expression elements |
| US7824907B2 (en) * | 2003-03-11 | 2010-11-02 | Merck Serono Sa | Expression vectors comprising the mCMV IE2 promoter |
| US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| KR101271884B1 (ko) | 2004-11-08 | 2013-06-05 | 크로마제닉스 비.브이. | 고수준으로 단백질을 발현하는 숙주세포의 선택 |
| US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| ES2384391T3 (es) | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
| US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| DE602006008752D1 (de) * | 2005-03-05 | 2009-10-08 | Millipore Corp | Neue regulatorische elemente enthaltende vektoren |
| GB0504587D0 (en) * | 2005-03-05 | 2005-04-13 | Ml Lab Plc | Vectors comprising guinea pig CMV regulatory elements |
| GB0509965D0 (en) | 2005-05-17 | 2005-06-22 | Ml Lab Plc | Improved expression elements |
| EP1739179A1 (en) * | 2005-06-30 | 2007-01-03 | Octapharma AG | Serum-free stable transfection and production of recombinant human proteins in human cell lines |
| US7968700B2 (en) | 2006-03-20 | 2011-06-28 | Chromagenics B.V. | Expression augmenting DNA fragments, use thereof, and methods for finding thereof |
| KR20110119702A (ko) * | 2009-01-22 | 2011-11-02 | 모멘타 파머슈티컬스 인코포레이티드 | Ｃｈｏ 세포에서 유도된 글리코단백질 생성물에서의 갈락토오스-알파-1,3-갈락토오스-함유 ｎ-글리칸 |
| CA2894511C (en) | 2012-12-11 | 2021-12-07 | Albert Einstein College Of Medicine Of Yeshiva University | Methods for high throughput receptor:ligand identification |
| WO2014134412A1 (en) * | 2013-03-01 | 2014-09-04 | Regents Of The University Of Minnesota | Talen-based gene correction |
| CN104341503A (zh) * | 2013-07-29 | 2015-02-11 | 西藏海思科药业集团股份有限公司 | 对蒙古人种和高加索人种低免疫原性的、抗cd20的人抗体 |
| CN104341505A (zh) * | 2013-07-29 | 2015-02-11 | 西藏海思科药业集团股份有限公司 | 对蒙古人种和高加索人种低免疫原性的、抗egfr的人鼠嵌合抗体 |
| SG11201605632SA (en) * | 2014-01-21 | 2016-08-30 | Einstein Coll Med | Cellular platform for rapid and comprehensive t-cell immunomonitoring |
| CN109689096A (zh) | 2016-05-18 | 2019-04-26 | 阿尔伯特爱因斯坦医学院公司 | 变体pd-l1多肽、t细胞调节性多聚体多肽及其使用方法 |
| WO2017201210A1 (en) | 2016-05-18 | 2017-11-23 | Cue Biopharma, Inc. | T-cell modulatory multimeric polypeptides and methods of use thereof |
| ES2973548T3 (es) | 2016-12-22 | 2024-06-20 | Cue Biopharma Inc | Polipéptidos multiméricos moduladores de linfocitos T y métodos para su uso |
| EP3565829A4 (en) | 2017-01-09 | 2021-01-27 | Cue Biopharma, Inc. | T-CELL-MODULATING MULTIMERIC POLYPEPTIDES AND METHOD OF USING THEREOF |
| US20200010528A1 (en) | 2017-03-15 | 2020-01-09 | Cue Biopharma, Inc. | Methods for modulating an immune response |
| CN111886241A (zh) | 2018-01-09 | 2020-11-03 | 库尔生物制药有限公司 | 多聚体t细胞调节多肽及其使用方法 |
| US11781146B2 (en) | 2018-05-24 | 2023-10-10 | National University Corporation Hokkaido University | Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element |
| WO2021231376A2 (en) | 2020-05-12 | 2021-11-18 | Cue Biopharma, Inc. | Multimeric t-cell modulatory polypeptides and methods of use thereof |
| WO2022056014A1 (en) | 2020-09-09 | 2022-03-17 | Cue Biopharma, Inc. | Mhc class ii t-cell modulatory multimeric polypeptides for treating type 1 diabetes mellitus (t1d) and methods of use thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO162160C (no) * | 1987-01-09 | 1989-11-15 | Medi Cult As | Serumfritt vekstmedium, samt anvendelse derav. |
| IL87737A (en) * | 1987-09-11 | 1993-08-18 | Genentech Inc | Method for culturing polypeptide factor dependent vertebrate recombinant cells |
| SE9303601D0 (sv) * | 1993-11-01 | 1993-11-01 | Kabi Pharmacia Ab | Improved cell cultivation method and medium |
| AU771111B2 (en) * | 1998-07-21 | 2004-03-11 | Emd Millipore Corporation | A polynucleotide comprising a ubiquitous chromatin opening element (UCOE) |
-
2002
- 2002-06-04 KR KR10-2003-7015872A patent/KR20040032105A/ko not_active Withdrawn
- 2002-06-04 CA CA002463310A patent/CA2463310A1/en not_active Abandoned
- 2002-06-04 EP EP02734688A patent/EP1402006A4/en not_active Withdrawn
- 2002-06-04 US US10/163,863 patent/US20040161817A1/en not_active Abandoned
- 2002-06-04 WO PCT/US2002/017763 patent/WO2002099089A1/en not_active Ceased
- 2002-06-04 CN CNA028143825A patent/CN1533432A/zh active Pending
- 2002-06-04 JP JP2003502199A patent/JP2004535189A/ja active Pending
- 2002-06-04 AU AU2002310321A patent/AU2002310321A1/en not_active Abandoned
- 2002-06-04 WO PCT/US2002/017770 patent/WO2002099070A2/en not_active Ceased
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