JP2004529621A5

JP2004529621A5 -

Download PDF

Info

Publication number: JP2004529621A5
Authority: JP; Japan
Prior art keywords: cells; cell; masc; mascs; differentiation
Prior art date: 2002-02-14
Legal status : Withdrawn

Application number

JP2002565063A

Other languages

English (en)

Japanese (ja)

Other versions

JP2004529621A (ja

Filing date

2002-02-14

Publication date

2007-09-20

2002-02-14 Application filed filed Critical

2002-02-14 Priority claimed from PCT/US2002/004652 external-priority patent/WO2002064748A2/en

2004-09-30 Publication of JP2004529621A publication Critical patent/JP2004529621A/ja

2007-09-20 Publication of JP2004529621A5 publication Critical patent/JP2004529621A5/ja

Status Withdrawn legal-status Critical Current

Links

210000004027 cell Anatomy 0.000 claims description 904
210000001519 tissue Anatomy 0.000 claims description 146
230000004069 differentiation Effects 0.000 claims description 134
210000001185 bone marrow Anatomy 0.000 claims description 123
241000282414 Homo sapiens Species 0.000 claims description 94
238000000034 method Methods 0.000 claims description 84
101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 claims description 66
102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 claims description 66
108090000623 proteins and genes Proteins 0.000 claims description 65
230000014509 gene expression Effects 0.000 claims description 63
210000004556 brain Anatomy 0.000 claims description 61
210000002569 neuron Anatomy 0.000 claims description 60
210000004185 liver Anatomy 0.000 claims description 58
238000001727 in vivo Methods 0.000 claims description 52
210000004504 adult stem cell Anatomy 0.000 claims description 46
210000003205 muscle Anatomy 0.000 claims description 43
210000003038 endothelium Anatomy 0.000 claims description 40
210000004369 blood Anatomy 0.000 claims description 33
239000008280 blood Substances 0.000 claims description 33
239000000427 antigen Substances 0.000 claims description 32
108091007433 antigens Proteins 0.000 claims description 31
102000036639 antigens Human genes 0.000 claims description 31
210000004248 oligodendroglia Anatomy 0.000 claims description 31
239000000203 mixture Substances 0.000 claims description 29
241000124008 Mammalia Species 0.000 claims description 25
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 23
210000002027 skeletal muscle Anatomy 0.000 claims description 23
210000002459 blastocyst Anatomy 0.000 claims description 22
201000010099 disease Diseases 0.000 claims description 22
210000000963 osteoblast Anatomy 0.000 claims description 21
102100032912 CD44 antigen Human genes 0.000 claims description 20
101000868273 Homo sapiens CD44 antigen Proteins 0.000 claims description 20
210000004602 germ cell Anatomy 0.000 claims description 20
210000004498 neuroglial cell Anatomy 0.000 claims description 20
210000001612 chondrocyte Anatomy 0.000 claims description 18
210000001789 adipocyte Anatomy 0.000 claims description 17
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 16
210000004204 blood vessel Anatomy 0.000 claims description 15
238000012258 culturing Methods 0.000 claims description 15
210000004700 fetal blood Anatomy 0.000 claims description 15
238000004519 manufacturing process Methods 0.000 claims description 15
230000001225 therapeutic effect Effects 0.000 claims description 15
210000002950 fibroblast Anatomy 0.000 claims description 14
210000002826 placenta Anatomy 0.000 claims description 13
108010017842 Telomerase Proteins 0.000 claims description 12
210000000981 epithelium Anatomy 0.000 claims description 11
230000001605 fetal effect Effects 0.000 claims description 11
210000004165 myocardium Anatomy 0.000 claims description 11
210000002460 smooth muscle Anatomy 0.000 claims description 11
210000001671 embryonic stem cell Anatomy 0.000 claims description 10
239000000306 component Substances 0.000 claims description 9
230000008520 organization Effects 0.000 claims description 9
210000000496 pancreas Anatomy 0.000 claims description 9
239000012503 blood component Substances 0.000 claims description 7
230000000747 cardiac effect Effects 0.000 claims description 7
230000011132 hemopoiesis Effects 0.000 claims description 7
206010061218 Inflammation Diseases 0.000 claims description 5
230000002950 deficient Effects 0.000 claims description 5
210000003743 erythrocyte Anatomy 0.000 claims description 5
230000004054 inflammatory process Effects 0.000 claims description 5
210000000265 leukocyte Anatomy 0.000 claims description 5
239000011159 matrix material Substances 0.000 claims description 5
210000001508 eye Anatomy 0.000 claims description 4
208000028867 ischemia Diseases 0.000 claims description 4
239000000463 material Substances 0.000 claims description 4
239000000758 substrate Substances 0.000 claims description 4
108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
210000000436 anus Anatomy 0.000 claims description 3
210000003238 esophagus Anatomy 0.000 claims description 3
210000000214 mouth Anatomy 0.000 claims description 3
210000004400 mucous membrane Anatomy 0.000 claims description 3
150000007523 nucleic acids Chemical group 0.000 claims description 3
238000007911 parenteral administration Methods 0.000 claims description 3
238000001356 surgical procedure Methods 0.000 claims description 3
238000012546 transfer Methods 0.000 claims description 3
210000001215 vagina Anatomy 0.000 claims description 3
JWZZKOKVBUJMES-UHFFFAOYSA-N (+-)-Isoprenaline Chemical compound CC(C)NCC(O)C1=CC=C(O)C(O)=C1 JWZZKOKVBUJMES-UHFFFAOYSA-N 0.000 claims description 2
102000043129 MHC class I family Human genes 0.000 claims description 2
108091054437 MHC class I family Proteins 0.000 claims description 2
102000043131 MHC class II family Human genes 0.000 claims description 2
108091054438 MHC class II family Proteins 0.000 claims description 2
210000001772 blood platelet Anatomy 0.000 claims description 2
239000003937 drug carrier Substances 0.000 claims description 2
210000003754 fetus Anatomy 0.000 claims description 2
208000018262 Peripheral vascular disease Diseases 0.000 claims 1
208000002223 abdominal aortic aneurysm Diseases 0.000 claims 1
208000007474 aortic aneurysm Diseases 0.000 claims 1
239000002473 artificial blood Substances 0.000 claims 1
230000035935 pregnancy Effects 0.000 claims 1
208000019553 vascular disease Diseases 0.000 claims 1
210000000130 stem cell Anatomy 0.000 description 125
210000003494 hepatocyte Anatomy 0.000 description 102
210000002889 endothelial cell Anatomy 0.000 description 85
241000699666 Mus <mouse, genus> Species 0.000 description 81
210000000056 organ Anatomy 0.000 description 77
241001465754 Metazoa Species 0.000 description 58
210000003958 hematopoietic stem cell Anatomy 0.000 description 58
108020004999 messenger RNA Proteins 0.000 description 50
229940050528 albumin Drugs 0.000 description 48
238000010186 staining Methods 0.000 description 48
102000009027 Albumins Human genes 0.000 description 47
108010088751 Albumins Proteins 0.000 description 47
241000699670 Mus sp. Species 0.000 description 47
VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical group C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 47
102100028072 Fibroblast growth factor 4 Human genes 0.000 description 46
101800003838 Epidermal growth factor Proteins 0.000 description 44
102100033237 Pro-epidermal growth factor Human genes 0.000 description 44
229940116977 epidermal growth factor Drugs 0.000 description 44
238000002054 transplantation Methods 0.000 description 44
239000002609 medium Substances 0.000 description 43
101001060274 Homo sapiens Fibroblast growth factor 4 Proteins 0.000 description 42
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 40
238000000338 in vitro Methods 0.000 description 40
102000003745 Hepatocyte Growth Factor Human genes 0.000 description 38
108090000100 Hepatocyte Growth Factor Proteins 0.000 description 38
241000700159 Rattus Species 0.000 description 37
108010005774 beta-Galactosidase Proteins 0.000 description 37
101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 description 36
102000003974 Fibroblast growth factor 2 Human genes 0.000 description 35
108090000379 Fibroblast growth factor 2 Proteins 0.000 description 35
102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 description 35
230000001537 neural effect Effects 0.000 description 35
206010028980 Neoplasm Diseases 0.000 description 33
WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 33
102100037362 Fibronectin Human genes 0.000 description 32
108010067306 Fibronectins Proteins 0.000 description 32
210000003716 mesoderm Anatomy 0.000 description 32
238000011282 treatment Methods 0.000 description 32
108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 31
102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 31
108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 31
230000003511 endothelial effect Effects 0.000 description 30
108091005250 Glycophorins Proteins 0.000 description 29
102000004058 Leukemia inhibitory factor Human genes 0.000 description 29
108090000581 Leukemia inhibitory factor Proteins 0.000 description 29
102100035716 Glycophorin-A Human genes 0.000 description 28
238000004458 analytical method Methods 0.000 description 28
230000000694 effects Effects 0.000 description 28
210000001178 neural stem cell Anatomy 0.000 description 27
108010047303 von Willebrand Factor Proteins 0.000 description 26
102100036537 von Willebrand factor Human genes 0.000 description 26
229960001134 von willebrand factor Drugs 0.000 description 26
241000282412 Homo Species 0.000 description 25
-1 glycoconjugate Proteins 0.000 description 25
102000004169 proteins and genes Human genes 0.000 description 25
210000001130 astrocyte Anatomy 0.000 description 24
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 24
210000002901 mesenchymal stem cell Anatomy 0.000 description 24
230000033115 angiogenesis Effects 0.000 description 23
230000032823 cell division Effects 0.000 description 23
230000006870 function Effects 0.000 description 23
230000003394 haemopoietic effect Effects 0.000 description 23
210000001900 endoderm Anatomy 0.000 description 22
238000002347 injection Methods 0.000 description 22
239000007924 injection Substances 0.000 description 22
238000012360 testing method Methods 0.000 description 22
101001027406 Danio rerio Fibroblast growth factor 8b Proteins 0.000 description 21
230000001177 retroviral effect Effects 0.000 description 21
108091035539 telomere Proteins 0.000 description 21
102000055501 telomere Human genes 0.000 description 21
210000003411 telomere Anatomy 0.000 description 21
210000000988 bone and bone Anatomy 0.000 description 20
239000004202 carbamide Substances 0.000 description 20
102000053171 Glial Fibrillary Acidic Human genes 0.000 description 19
101710193519 Glial fibrillary acidic protein Proteins 0.000 description 19
210000005046 glial fibrillary acidic protein Anatomy 0.000 description 19
108010082117 matrigel Proteins 0.000 description 19
235000018102 proteins Nutrition 0.000 description 19
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 18
108010081589 Becaplermin Proteins 0.000 description 18
102000040945 Transcription factor Human genes 0.000 description 18
108091023040 Transcription factor Proteins 0.000 description 18
102000016549 Vascular Endothelial Growth Factor Receptor-2 Human genes 0.000 description 18
210000002919 epithelial cell Anatomy 0.000 description 18
BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 18
210000004072 lung Anatomy 0.000 description 18
108091003079 Bovine Serum Albumin Proteins 0.000 description 17
102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 17
101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 17
101150088608 Kdr gene Proteins 0.000 description 17
239000012679 serum free medium Substances 0.000 description 17
210000000952 spleen Anatomy 0.000 description 17
238000001262 western blot Methods 0.000 description 17
102100021747 Leukemia inhibitory factor receptor Human genes 0.000 description 16
108010064527 OSM-LIF Receptors Proteins 0.000 description 16
210000000845 cartilage Anatomy 0.000 description 16
239000000047 product Substances 0.000 description 16
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 15
102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 15
UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 15
229960003957 dexamethasone Drugs 0.000 description 15
210000002216 heart Anatomy 0.000 description 15
210000005260 human cell Anatomy 0.000 description 15
238000003364 immunohistochemistry Methods 0.000 description 15
210000001020 neural plate Anatomy 0.000 description 15
229940098773 bovine serum albumin Drugs 0.000 description 14
238000003753 real-time PCR Methods 0.000 description 14
102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 description 13
229930193140 Neomycin Natural products 0.000 description 13
230000009762 endothelial cell differentiation Effects 0.000 description 13
210000003237 epithelioid cell Anatomy 0.000 description 13
229960004927 neomycin Drugs 0.000 description 13
210000001778 pluripotent stem cell Anatomy 0.000 description 13
108010020076 Cytochrome P-450 CYP2B1 Proteins 0.000 description 12
102000004127 Cytokines Human genes 0.000 description 12
108090000695 Cytokines Proteins 0.000 description 12
108020004414 DNA Proteins 0.000 description 12
NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 12
101000998011 Homo sapiens Keratin, type I cytoskeletal 19 Proteins 0.000 description 12
102000004877 Insulin Human genes 0.000 description 12
108090001061 Insulin Proteins 0.000 description 12
108700018351 Major Histocompatibility Complex Proteins 0.000 description 12
101710150336 Protein Rex Proteins 0.000 description 12
230000008901 benefit Effects 0.000 description 12
230000002068 genetic effect Effects 0.000 description 12
229940125396 insulin Drugs 0.000 description 12
210000002966 serum Anatomy 0.000 description 12
239000011734 sodium Substances 0.000 description 12
230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 12
230000002792 vascular Effects 0.000 description 12
108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 11
102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 11
239000012983 Dulbecco’s minimal essential medium Substances 0.000 description 11
108060008487 Myosin Proteins 0.000 description 11
102000003505 Myosin Human genes 0.000 description 11
108091000117 Tyrosine 3-Monooxygenase Proteins 0.000 description 11
102000048218 Tyrosine 3-monooxygenases Human genes 0.000 description 11
239000002299 complementary DNA Substances 0.000 description 11
230000001419 dependent effect Effects 0.000 description 11
230000006698 induction Effects 0.000 description 11
210000005087 mononuclear cell Anatomy 0.000 description 11
230000037361 pathway Effects 0.000 description 11
238000011160 research Methods 0.000 description 11
239000000243 solution Substances 0.000 description 11
102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 10
LEBBDRXHHNYZIA-LDUWYPJVSA-N [(2s,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] n-[(z)-1,3-dihydroxyoctadec-4-en-2-yl]carbamate Chemical compound CCCCCCCCCCCCC\C=C/C(O)C(CO)NC(=O)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O LEBBDRXHHNYZIA-LDUWYPJVSA-N 0.000 description 10
230000001464 adherent effect Effects 0.000 description 10
230000013020 embryo development Effects 0.000 description 10
238000002474 experimental method Methods 0.000 description 10
230000012010 growth Effects 0.000 description 10
210000000987 immune system Anatomy 0.000 description 10
230000001939 inductive effect Effects 0.000 description 10
102000005962 receptors Human genes 0.000 description 10
108020003175 receptors Proteins 0.000 description 10
230000009278 visceral effect Effects 0.000 description 10
OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 9
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
108010045374 CD36 Antigens Proteins 0.000 description 9
102000053028 CD36 Antigens Human genes 0.000 description 9
102100022057 Hepatocyte nuclear factor 1-alpha Human genes 0.000 description 9
101001045751 Homo sapiens Hepatocyte nuclear factor 1-alpha Proteins 0.000 description 9
101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 description 9
238000001190 Q-PCR Methods 0.000 description 9
102100021380 Transcription factor GATA-4 Human genes 0.000 description 9
241000700605 Viruses Species 0.000 description 9
229960005070 ascorbic acid Drugs 0.000 description 9
201000011510 cancer Diseases 0.000 description 9
238000011161 development Methods 0.000 description 9
230000018109 developmental process Effects 0.000 description 9
238000010790 dilution Methods 0.000 description 9
239000012895 dilution Substances 0.000 description 9
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 9
210000005064 dopaminergic neuron Anatomy 0.000 description 9
239000003814 drug Substances 0.000 description 9
210000002257 embryonic structure Anatomy 0.000 description 9
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 9
238000000799 fluorescence microscopy Methods 0.000 description 9
229960003692 gamma aminobutyric acid Drugs 0.000 description 9
210000003566 hemangioblast Anatomy 0.000 description 9
DDBREPKUVSBGFI-UHFFFAOYSA-N phenobarbital Chemical compound C=1C=CC=CC=1C1(CC)C(=O)NC(=O)NC1=O DDBREPKUVSBGFI-UHFFFAOYSA-N 0.000 description 9
229960002695 phenobarbital Drugs 0.000 description 9
239000002953 phosphate buffered saline Substances 0.000 description 9
238000003752 polymerase chain reaction Methods 0.000 description 9
210000003491 skin Anatomy 0.000 description 9
241000894007 species Species 0.000 description 9
239000000126 substance Substances 0.000 description 9
108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
102000010834 Extracellular Matrix Proteins Human genes 0.000 description 8
108010037362 Extracellular Matrix Proteins Proteins 0.000 description 8
206010021143 Hypoxia Diseases 0.000 description 8
108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 8
102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 8
235000010323 ascorbic acid Nutrition 0.000 description 8
239000011668 ascorbic acid Substances 0.000 description 8
210000000349 chromosome Anatomy 0.000 description 8
210000002744 extracellular matrix Anatomy 0.000 description 8
210000000936 intestine Anatomy 0.000 description 8
208000019423 liver disease Diseases 0.000 description 8
239000003550 marker Substances 0.000 description 8
229910052711 selenium Inorganic materials 0.000 description 8
239000011669 selenium Substances 0.000 description 8
QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 8
239000013598 vector Substances 0.000 description 8
OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 7
OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 7
108010085238 Actins Proteins 0.000 description 7
102000007469 Actins Human genes 0.000 description 7
101100257359 Caenorhabditis elegans sox-2 gene Proteins 0.000 description 7
108091006146 Channels Proteins 0.000 description 7
101150048336 Flt1 gene Proteins 0.000 description 7
229920002527 Glycogen Polymers 0.000 description 7
101000610551 Homo sapiens Prominin-1 Proteins 0.000 description 7
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 7
101100257363 Mus musculus Sox2 gene Proteins 0.000 description 7
102000008730 Nestin Human genes 0.000 description 7
108010088225 Nestin Proteins 0.000 description 7
229930040373 Paraformaldehyde Natural products 0.000 description 7
102100040120 Prominin-1 Human genes 0.000 description 7
BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 7
102000004338 Transferrin Human genes 0.000 description 7
108090000901 Transferrin Proteins 0.000 description 7
230000003321 amplification Effects 0.000 description 7
229960002756 azacitidine Drugs 0.000 description 7
102000015736 beta 2-Microglobulin Human genes 0.000 description 7
108010081355 beta 2-Microglobulin Proteins 0.000 description 7
210000003169 central nervous system Anatomy 0.000 description 7
206010012601 diabetes mellitus Diseases 0.000 description 7
229940079593 drug Drugs 0.000 description 7
238000005516 engineering process Methods 0.000 description 7
230000003371 gabaergic effect Effects 0.000 description 7
229940096919 glycogen Drugs 0.000 description 7
239000003102 growth factor Substances 0.000 description 7
230000002440 hepatic effect Effects 0.000 description 7
238000011534 incubation Methods 0.000 description 7
238000003780 insertion Methods 0.000 description 7
230000037431 insertion Effects 0.000 description 7
210000003734 kidney Anatomy 0.000 description 7
210000001161 mammalian embryo Anatomy 0.000 description 7
238000001000 micrograph Methods 0.000 description 7
210000003098 myoblast Anatomy 0.000 description 7
210000005055 nestin Anatomy 0.000 description 7
238000003199 nucleic acid amplification method Methods 0.000 description 7
229920002866 paraformaldehyde Polymers 0.000 description 7
230000008569 process Effects 0.000 description 7
230000004044 response Effects 0.000 description 7
239000012581 transferrin Substances 0.000 description 7
KISWVXRQTGLFGD-UHFFFAOYSA-N 2-[[2-[[6-amino-2-[[2-[[2-[[5-amino-2-[[2-[[1-[2-[[6-amino-2-[(2,5-diamino-5-oxopentanoyl)amino]hexanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-(diaminomethylideneamino)p Chemical compound C1CCN(C(=O)C(CCCN=C(N)N)NC(=O)C(CCCCN)NC(=O)C(N)CCC(N)=O)C1C(=O)NC(CO)C(=O)NC(CCC(N)=O)C(=O)NC(CCCN=C(N)N)C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC(C)C)C(O)=O)CC1=CC=C(O)C=C1 KISWVXRQTGLFGD-UHFFFAOYSA-N 0.000 description 6
NMUSYJAQQFHJEW-KVTDHHQDSA-N 5-azacytidine Chemical compound O=C1N=C(N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 NMUSYJAQQFHJEW-KVTDHHQDSA-N 0.000 description 6
102100022749 Aminopeptidase N Human genes 0.000 description 6
102000004219 Brain-derived neurotrophic factor Human genes 0.000 description 6
108090000715 Brain-derived neurotrophic factor Proteins 0.000 description 6
206010068051 Chimerism Diseases 0.000 description 6
102100023471 E-selectin Human genes 0.000 description 6
101000757160 Homo sapiens Aminopeptidase N Proteins 0.000 description 6
208000026350 Inborn Genetic disease Diseases 0.000 description 6
108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 6
102000014429 Insulin-like growth factor Human genes 0.000 description 6
101100440874 Mus musculus Cyp2b9 gene Proteins 0.000 description 6
238000011789 NOD SCID mouse Methods 0.000 description 6
108060005874 Parvalbumin Proteins 0.000 description 6
102000001675 Parvalbumin Human genes 0.000 description 6
102100038021 Steryl-sulfatase Human genes 0.000 description 6
241000282887 Suidae Species 0.000 description 6
102000008790 VE-cadherin Human genes 0.000 description 6
230000004913 activation Effects 0.000 description 6
238000001994 activation Methods 0.000 description 6
229940077737 brain-derived neurotrophic factor Drugs 0.000 description 6
108010018828 cadherin 5 Proteins 0.000 description 6
210000004413 cardiac myocyte Anatomy 0.000 description 6
238000001218 confocal laser scanning microscopy Methods 0.000 description 6
230000006378 damage Effects 0.000 description 6
230000007812 deficiency Effects 0.000 description 6
230000003291 dopaminomimetic effect Effects 0.000 description 6
208000016361 genetic disease Diseases 0.000 description 6
229930195712 glutamate Natural products 0.000 description 6
229960001340 histamine Drugs 0.000 description 6
230000007954 hypoxia Effects 0.000 description 6
208000015181 infectious disease Diseases 0.000 description 6
230000000877 morphologic effect Effects 0.000 description 6
238000010899 nucleation Methods 0.000 description 6
230000000862 serotonergic effect Effects 0.000 description 6
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
238000010361 transduction Methods 0.000 description 6
230000026683 transduction Effects 0.000 description 6
230000005747 tumor angiogenesis Effects 0.000 description 6
NMUSYJAQQFHJEW-UHFFFAOYSA-N 5-Azacytidine Natural products O=C1N=C(N)N=CN1C1C(O)C(O)C(CO)O1 NMUSYJAQQFHJEW-UHFFFAOYSA-N 0.000 description 5
208000023275 Autoimmune disease Diseases 0.000 description 5
102000008186 Collagen Human genes 0.000 description 5
108010035532 Collagen Proteins 0.000 description 5
108010020070 Cytochrome P-450 CYP2B6 Proteins 0.000 description 5
102000009666 Cytochrome P-450 CYP2B6 Human genes 0.000 description 5
238000002965 ELISA Methods 0.000 description 5
102000018233 Fibroblast Growth Factor Human genes 0.000 description 5
108050007372 Fibroblast Growth Factor Proteins 0.000 description 5
208000031220 Hemophilia Diseases 0.000 description 5
208000009292 Hemophilia A Diseases 0.000 description 5
101000622123 Homo sapiens E-selectin Proteins 0.000 description 5
101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 5
102000007330 LDL Lipoproteins Human genes 0.000 description 5
108010007622 LDL Lipoproteins Proteins 0.000 description 5
101150094768 Mcam gene Proteins 0.000 description 5
102000047918 Myelin Basic Human genes 0.000 description 5
101710107068 Myelin basic protein Proteins 0.000 description 5
108090000630 Oncostatin M Proteins 0.000 description 5
102000004140 Oncostatin M Human genes 0.000 description 5
102100023472 P-selectin Human genes 0.000 description 5
241000283984 Rodentia Species 0.000 description 5
206010043276 Teratoma Diseases 0.000 description 5
102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 5
208000027418 Wounds and injury Diseases 0.000 description 5
208000037919 acquired disease Diseases 0.000 description 5
230000000735 allogeneic effect Effects 0.000 description 5
230000015572 biosynthetic process Effects 0.000 description 5
230000024245 cell differentiation Effects 0.000 description 5
230000010261 cell growth Effects 0.000 description 5
230000008859 change Effects 0.000 description 5
238000012512 characterization method Methods 0.000 description 5
229920001436 collagen Polymers 0.000 description 5
229940126864 fibroblast growth factor Drugs 0.000 description 5
210000001222 gaba-ergic neuron Anatomy 0.000 description 5
210000001035 gastrointestinal tract Anatomy 0.000 description 5
238000001415 gene therapy Methods 0.000 description 5
230000002518 glial effect Effects 0.000 description 5
239000008103 glucose Substances 0.000 description 5
230000028993 immune response Effects 0.000 description 5
230000002055 immunohistochemical effect Effects 0.000 description 5
238000002955 isolation Methods 0.000 description 5
210000004379 membrane Anatomy 0.000 description 5
239000012528 membrane Substances 0.000 description 5
210000001259 mesencephalon Anatomy 0.000 description 5
210000003061 neural cell Anatomy 0.000 description 5
239000002773 nucleotide Substances 0.000 description 5
125000003729 nucleotide group Chemical group 0.000 description 5
239000002243 precursor Substances 0.000 description 5
229940091258 selenium supplement Drugs 0.000 description 5
238000000926 separation method Methods 0.000 description 5
238000003860 storage Methods 0.000 description 5
230000001052 transient effect Effects 0.000 description 5
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
102100038238 Aromatic-L-amino-acid decarboxylase Human genes 0.000 description 4
102100023126 Cell surface glycoprotein MUC18 Human genes 0.000 description 4
208000035473 Communicable disease Diseases 0.000 description 4
108090000386 Fibroblast Growth Factor 1 Proteins 0.000 description 4
102100031706 Fibroblast growth factor 1 Human genes 0.000 description 4
108090000381 Fibroblast growth factor 4 Proteins 0.000 description 4
108090000385 Fibroblast growth factor 7 Proteins 0.000 description 4
102000003972 Fibroblast growth factor 7 Human genes 0.000 description 4
238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 4
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
102000018899 Glutamate Receptors Human genes 0.000 description 4
108010027915 Glutamate Receptors Proteins 0.000 description 4
DHCLVCXQIBBOPH-UHFFFAOYSA-N Glycerol 2-phosphate Chemical compound OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 description 4
208000005176 Hepatitis C Diseases 0.000 description 4
101000623903 Homo sapiens Cell surface glycoprotein MUC18 Proteins 0.000 description 4
101000622137 Homo sapiens P-selectin Proteins 0.000 description 4
108091006905 Human Serum Albumin Proteins 0.000 description 4
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
102000011782 Keratins Human genes 0.000 description 4
108010076876 Keratins Proteins 0.000 description 4
101150039183 MYF6 gene Proteins 0.000 description 4
102000009664 Microtubule-Associated Proteins Human genes 0.000 description 4
108010020004 Microtubule-Associated Proteins Proteins 0.000 description 4
102100038379 Myogenic factor 6 Human genes 0.000 description 4
101150053185 P450 gene Proteins 0.000 description 4
YASAKCUCGLMORW-UHFFFAOYSA-N Rosiglitazone Chemical compound C=1C=CC=NC=1N(C)CCOC(C=C1)=CC=C1CC1SC(=O)NC1=O YASAKCUCGLMORW-UHFFFAOYSA-N 0.000 description 4
108700019146 Transgenes Proteins 0.000 description 4
206010052779 Transplant rejections Diseases 0.000 description 4
102000004142 Trypsin Human genes 0.000 description 4
108090000631 Trypsin Proteins 0.000 description 4
108010035075 Tyrosine decarboxylase Proteins 0.000 description 4
230000002491 angiogenic effect Effects 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
238000003501 co-culture Methods 0.000 description 4
238000004624 confocal microscopy Methods 0.000 description 4
210000004748 cultured cell Anatomy 0.000 description 4
230000003412 degenerative effect Effects 0.000 description 4
238000007876 drug discovery Methods 0.000 description 4
239000000975 dye Substances 0.000 description 4
238000011156 evaluation Methods 0.000 description 4
210000003414 extremity Anatomy 0.000 description 4
235000019197 fats Nutrition 0.000 description 4
210000001654 germ layer Anatomy 0.000 description 4
239000011521 glass Substances 0.000 description 4
238000002991 immunohistochemical analysis Methods 0.000 description 4
239000003018 immunosuppressive agent Substances 0.000 description 4
208000014674 injury Diseases 0.000 description 4
230000000968 intestinal effect Effects 0.000 description 4
239000010410 layer Substances 0.000 description 4
210000004698 lymphocyte Anatomy 0.000 description 4
230000035800 maturation Effects 0.000 description 4
230000001404 mediated effect Effects 0.000 description 4
210000002241 neurite Anatomy 0.000 description 4
230000004031 neuronal differentiation Effects 0.000 description 4
230000001105 regulatory effect Effects 0.000 description 4
229940076279 serotonin Drugs 0.000 description 4
210000000329 smooth muscle myocyte Anatomy 0.000 description 4
229910052708 sodium Inorganic materials 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
238000002560 therapeutic procedure Methods 0.000 description 4
230000036962 time dependent Effects 0.000 description 4
239000012588 trypsin Substances 0.000 description 4
241001430294 unidentified retrovirus Species 0.000 description 4
230000003827 upregulation Effects 0.000 description 4
210000005166 vasculature Anatomy 0.000 description 4
230000029663 wound healing Effects 0.000 description 4
238000002689 xenotransplantation Methods 0.000 description 4
108010007726 Bone Morphogenetic Proteins Proteins 0.000 description 3
102000007350 Bone Morphogenetic Proteins Human genes 0.000 description 3
102000017420 CD3 protein, epsilon/gamma/delta subunit Human genes 0.000 description 3
108050005493 CD3 protein, epsilon/gamma/delta subunit Proteins 0.000 description 3
241000283707 Capra Species 0.000 description 3
208000024172 Cardiovascular disease Diseases 0.000 description 3
102100027417 Cytochrome P450 1B1 Human genes 0.000 description 3
102100036912 Desmin Human genes 0.000 description 3
108010044052 Desmin Proteins 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
102000003951 Erythropoietin Human genes 0.000 description 3
108090000394 Erythropoietin Proteins 0.000 description 3
102100028071 Fibroblast growth factor 7 Human genes 0.000 description 3
206010071602 Genetic polymorphism Diseases 0.000 description 3
206010018338 Glioma Diseases 0.000 description 3
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
101000725164 Homo sapiens Cytochrome P450 1B1 Proteins 0.000 description 3
101001060261 Homo sapiens Fibroblast growth factor 7 Proteins 0.000 description 3
101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 3
102100025304 Integrin beta-1 Human genes 0.000 description 3
WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 3
MIJPAVRNWPDMOR-ZAFYKAAXSA-N L-ascorbic acid 2-phosphate Chemical compound OC[C@H](O)[C@H]1OC(=O)C(OP(O)(O)=O)=C1O MIJPAVRNWPDMOR-ZAFYKAAXSA-N 0.000 description 3
108010085895 Laminin Proteins 0.000 description 3
102000007547 Laminin Human genes 0.000 description 3
OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 3
101000930477 Mus musculus Albumin Proteins 0.000 description 3
101100481406 Mus musculus Tie1 gene Proteins 0.000 description 3
102100038380 Myogenic factor 5 Human genes 0.000 description 3
101710099061 Myogenic factor 5 Proteins 0.000 description 3
HOKKHZGPKSLGJE-GSVOUGTGSA-N N-Methyl-D-aspartic acid Chemical compound CN[C@@H](C(O)=O)CC(O)=O HOKKHZGPKSLGJE-GSVOUGTGSA-N 0.000 description 3
101150026563 NR4A2 gene Proteins 0.000 description 3
102000003729 Neprilysin Human genes 0.000 description 3
108090000028 Neprilysin Proteins 0.000 description 3
241000283973 Oryctolagus cuniculus Species 0.000 description 3
101150044441 PECAM1 gene Proteins 0.000 description 3
208000018737 Parkinson disease Diseases 0.000 description 3
229930182555 Penicillin Natural products 0.000 description 3
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
238000011529 RT qPCR Methods 0.000 description 3
241000700157 Rattus norvegicus Species 0.000 description 3
102000004278 Receptor Protein-Tyrosine Kinases Human genes 0.000 description 3
108090000873 Receptor Protein-Tyrosine Kinases Proteins 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
102000004987 Troponin T Human genes 0.000 description 3
108090001108 Troponin T Proteins 0.000 description 3
108010073925 Vascular Endothelial Growth Factor B Proteins 0.000 description 3
102100038217 Vascular endothelial growth factor B Human genes 0.000 description 3
101710185494 Zinc finger protein Proteins 0.000 description 3
102100023597 Zinc finger protein 816 Human genes 0.000 description 3
230000005856 abnormality Effects 0.000 description 3
102000019997 adhesion receptor Human genes 0.000 description 3
108010013985 adhesion receptor Proteins 0.000 description 3
238000003556 assay Methods 0.000 description 3
210000003050 axon Anatomy 0.000 description 3
230000004888 barrier function Effects 0.000 description 3
210000002449 bone cell Anatomy 0.000 description 3
210000002798 bone marrow cell Anatomy 0.000 description 3
210000004271 bone marrow stromal cell Anatomy 0.000 description 3
229940112869 bone morphogenetic protein Drugs 0.000 description 3
210000005013 brain tissue Anatomy 0.000 description 3
230000004663 cell proliferation Effects 0.000 description 3
230000001413 cellular effect Effects 0.000 description 3
230000036755 cellular response Effects 0.000 description 3
238000002512 chemotherapy Methods 0.000 description 3
239000011248 coating agent Substances 0.000 description 3
238000000576 coating method Methods 0.000 description 3
210000000805 cytoplasm Anatomy 0.000 description 3
230000001086 cytosolic effect Effects 0.000 description 3
238000007405 data analysis Methods 0.000 description 3
230000003247 decreasing effect Effects 0.000 description 3
238000004925 denaturation Methods 0.000 description 3
230000036425 denaturation Effects 0.000 description 3
210000005045 desmin Anatomy 0.000 description 3
238000001514 detection method Methods 0.000 description 3
238000010586 diagram Methods 0.000 description 3
MHUWZNTUIIFHAS-CLFAGFIQSA-N dioleoyl phosphatidic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(O)=O)OC(=O)CCCCCCC\C=C/CCCCCCCC MHUWZNTUIIFHAS-CLFAGFIQSA-N 0.000 description 3
210000003981 ectoderm Anatomy 0.000 description 3
210000004039 endoderm cell Anatomy 0.000 description 3
229940088598 enzyme Drugs 0.000 description 3
230000000925 erythroid effect Effects 0.000 description 3
229940105423 erythropoietin Drugs 0.000 description 3
238000000684 flow cytometry Methods 0.000 description 3
239000007850 fluorescent dye Substances 0.000 description 3
238000011990 functional testing Methods 0.000 description 3
230000004927 fusion Effects 0.000 description 3
208000005017 glioblastoma Diseases 0.000 description 3
239000005090 green fluorescent protein Substances 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
208000019622 heart disease Diseases 0.000 description 3
210000003897 hepatic stem cell Anatomy 0.000 description 3
210000001320 hippocampus Anatomy 0.000 description 3
230000000642 iatrogenic effect Effects 0.000 description 3
238000010166 immunofluorescence Methods 0.000 description 3
229960003444 immunosuppressant agent Drugs 0.000 description 3
239000007943 implant Substances 0.000 description 3
238000002513 implantation Methods 0.000 description 3
230000001976 improved effect Effects 0.000 description 3
238000010348 incorporation Methods 0.000 description 3
230000002401 inhibitory effect Effects 0.000 description 3
210000004347 intestinal mucosa Anatomy 0.000 description 3
230000003834 intracellular effect Effects 0.000 description 3
229960004502 levodopa Drugs 0.000 description 3
229960004232 linoleic acid Drugs 0.000 description 3
OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 3
235000020778 linoleic acid Nutrition 0.000 description 3
210000005229 liver cell Anatomy 0.000 description 3
230000003908 liver function Effects 0.000 description 3
208000030159 metabolic disease Diseases 0.000 description 3
230000002503 metabolic effect Effects 0.000 description 3
230000004048 modification Effects 0.000 description 3
238000012986 modification Methods 0.000 description 3
210000005036 nerve Anatomy 0.000 description 3
108010091047 neurofilament protein H Proteins 0.000 description 3
108010062847 neurofilament protein NF 68 Proteins 0.000 description 3
210000004940 nucleus Anatomy 0.000 description 3
229920002113 octoxynol Polymers 0.000 description 3
230000005868 ontogenesis Effects 0.000 description 3
229940049954 penicillin Drugs 0.000 description 3
OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 3
238000002360 preparation method Methods 0.000 description 3
239000011535 reaction buffer Substances 0.000 description 3
230000002829 reductive effect Effects 0.000 description 3
230000002441 reversible effect Effects 0.000 description 3
239000000523 sample Substances 0.000 description 3
239000002356 single layer Substances 0.000 description 3
210000001057 smooth muscle myoblast Anatomy 0.000 description 3
238000012453 sprague-dawley rat model Methods 0.000 description 3
229960005322 streptomycin Drugs 0.000 description 3
210000003518 stress fiber Anatomy 0.000 description 3
230000004083 survival effect Effects 0.000 description 3
230000009885 systemic effect Effects 0.000 description 3
238000013518 transcription Methods 0.000 description 3
230000035897 transcription Effects 0.000 description 3
230000009261 transgenic effect Effects 0.000 description 3
230000000472 traumatic effect Effects 0.000 description 3
210000004881 tumor cell Anatomy 0.000 description 3
210000003462 vein Anatomy 0.000 description 3
239000013603 viral vector Substances 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
210000001325 yolk sac Anatomy 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
208000030507 AIDS Diseases 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
102100024222 B-lymphocyte antigen CD19 Human genes 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
108010022452 Collagen Type I Proteins 0.000 description 2
102000012422 Collagen Type I Human genes 0.000 description 2
102000000503 Collagen Type II Human genes 0.000 description 2
108010041390 Collagen Type II Proteins 0.000 description 2
102000004266 Collagen Type IV Human genes 0.000 description 2
108010042086 Collagen Type IV Proteins 0.000 description 2
208000020406 Creutzfeldt Jacob disease Diseases 0.000 description 2
208000010859 Creutzfeldt-Jakob disease Diseases 0.000 description 2
PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 description 2
108010036949 Cyclosporine Proteins 0.000 description 2
102000018832 Cytochromes Human genes 0.000 description 2
108010052832 Cytochromes Proteins 0.000 description 2
101100481404 Danio rerio tie1 gene Proteins 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
241000283074 Equus asinus Species 0.000 description 2
108700039887 Essential Genes Proteins 0.000 description 2
102100028412 Fibroblast growth factor 10 Human genes 0.000 description 2
108090001047 Fibroblast growth factor 10 Proteins 0.000 description 2
108090000368 Fibroblast growth factor 8 Proteins 0.000 description 2
102100037680 Fibroblast growth factor 8 Human genes 0.000 description 2
102100037665 Fibroblast growth factor 9 Human genes 0.000 description 2
108090000367 Fibroblast growth factor 9 Proteins 0.000 description 2
241000287828 Gallus gallus Species 0.000 description 2
102100030540 Gap junction alpha-5 protein Human genes 0.000 description 2
101150007884 Gata6 gene Proteins 0.000 description 2
208000032612 Glial tumor Diseases 0.000 description 2
102000006354 HLA-DR Antigens Human genes 0.000 description 2
108010058597 HLA-DR Antigens Proteins 0.000 description 2
206010019663 Hepatic failure Diseases 0.000 description 2
102100022054 Hepatocyte nuclear factor 4-alpha Human genes 0.000 description 2
101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 description 2
101001045740 Homo sapiens Hepatocyte nuclear factor 4-alpha Proteins 0.000 description 2
101000872458 Homo sapiens Huntingtin-interacting protein 1-related protein Proteins 0.000 description 2
101001078133 Homo sapiens Integrin alpha-2 Proteins 0.000 description 2
101000994369 Homo sapiens Integrin alpha-5 Proteins 0.000 description 2
101000599862 Homo sapiens Intercellular adhesion molecule 3 Proteins 0.000 description 2
101000835093 Homo sapiens Transferrin receptor protein 1 Proteins 0.000 description 2
101000801228 Homo sapiens Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 description 2
102000008100 Human Serum Albumin Human genes 0.000 description 2
102100034773 Huntingtin-interacting protein 1-related protein Human genes 0.000 description 2
206010062016 Immunosuppression Diseases 0.000 description 2
206010061216 Infarction Diseases 0.000 description 2
102100025305 Integrin alpha-2 Human genes 0.000 description 2
102100032817 Integrin alpha-5 Human genes 0.000 description 2
102100037871 Intercellular adhesion molecule 3 Human genes 0.000 description 2
102100033421 Keratin, type I cytoskeletal 18 Human genes 0.000 description 2
108010013563 Lipoprotein Lipase Proteins 0.000 description 2
102100022119 Lipoprotein lipase Human genes 0.000 description 2
102000008934 Muscle Proteins Human genes 0.000 description 2
108010074084 Muscle Proteins Proteins 0.000 description 2
102100032970 Myogenin Human genes 0.000 description 2
108010056785 Myogenin Proteins 0.000 description 2
208000012902 Nervous system disease Diseases 0.000 description 2
208000025966 Neurological disease Diseases 0.000 description 2
101150092239 OTX2 gene Proteins 0.000 description 2
108091034117 Oligonucleotide Proteins 0.000 description 2
102000004067 Osteocalcin Human genes 0.000 description 2
108090000573 Osteocalcin Proteins 0.000 description 2
102000009890 Osteonectin Human genes 0.000 description 2
108010077077 Osteonectin Proteins 0.000 description 2
206010033799 Paralysis Diseases 0.000 description 2
102000003992 Peroxidases Human genes 0.000 description 2
108090000029 Peroxisome Proliferator-Activated Receptors Proteins 0.000 description 2
102000003728 Peroxisome Proliferator-Activated Receptors Human genes 0.000 description 2
108010007568 Protamines Proteins 0.000 description 2
102000007327 Protamines Human genes 0.000 description 2
102100023088 Protein S100-A5 Human genes 0.000 description 2
108090000184 Selectins Proteins 0.000 description 2
102000003800 Selectins Human genes 0.000 description 2
102000019208 Serotonin Plasma Membrane Transport Proteins Human genes 0.000 description 2
108010012996 Serotonin Plasma Membrane Transport Proteins Proteins 0.000 description 2
210000001744 T-lymphocyte Anatomy 0.000 description 2
101150052863 THY1 gene Proteins 0.000 description 2
102000036693 Thrombopoietin Human genes 0.000 description 2
108010041111 Thrombopoietin Proteins 0.000 description 2
QHNORJFCVHUPNH-UHFFFAOYSA-L To-Pro-3 Chemical compound [I-].[I-].S1C2=CC=CC=C2[N+](C)=C1C=CC=C1C2=CC=CC=C2N(CCC[N+](C)(C)C)C=C1 QHNORJFCVHUPNH-UHFFFAOYSA-L 0.000 description 2
102100026144 Transferrin receptor protein 1 Human genes 0.000 description 2
102000046299 Transforming Growth Factor beta1 Human genes 0.000 description 2
101800002279 Transforming growth factor beta-1 Proteins 0.000 description 2
102000005506 Tryptophan Hydroxylase Human genes 0.000 description 2
108010031944 Tryptophan Hydroxylase Proteins 0.000 description 2
102000004243 Tubulin Human genes 0.000 description 2
108090000704 Tubulin Proteins 0.000 description 2
102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 description 2
108091008605 VEGF receptors Proteins 0.000 description 2
102000009484 Vascular Endothelial Growth Factor Receptors Human genes 0.000 description 2
230000002378 acidificating effect Effects 0.000 description 2
230000036982 action potential Effects 0.000 description 2
210000000577 adipose tissue Anatomy 0.000 description 2
SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 2
FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
229910021529 ammonia Inorganic materials 0.000 description 2
208000007502 anemia Diseases 0.000 description 2
239000002246 antineoplastic agent Substances 0.000 description 2
238000013459 approach Methods 0.000 description 2
210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 2
239000011324 bead Substances 0.000 description 2
230000009286 beneficial effect Effects 0.000 description 2
208000036815 beta tubulin Diseases 0.000 description 2
210000000013 bile duct Anatomy 0.000 description 2
230000027455 binding Effects 0.000 description 2
210000004703 blastocyst inner cell mass Anatomy 0.000 description 2
230000000903 blocking effect Effects 0.000 description 2
230000008499 blood brain barrier function Effects 0.000 description 2
210000000601 blood cell Anatomy 0.000 description 2
210000001218 blood-brain barrier Anatomy 0.000 description 2
238000010322 bone marrow transplantation Methods 0.000 description 2
239000011575 calcium Substances 0.000 description 2
229910052791 calcium Inorganic materials 0.000 description 2
239000000969 carrier Substances 0.000 description 2
230000011712 cell development Effects 0.000 description 2
239000006285 cell suspension Substances 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
229960001265 ciclosporin Drugs 0.000 description 2
210000003459 common hepatic duct Anatomy 0.000 description 2
230000000295 complement effect Effects 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
238000012790 confirmation Methods 0.000 description 2
210000002808 connective tissue Anatomy 0.000 description 2
208000018631 connective tissue disease Diseases 0.000 description 2
108010014510 connexin 40 Proteins 0.000 description 2
238000011109 contamination Methods 0.000 description 2
230000005574 cross-species transmission Effects 0.000 description 2
239000012228 culture supernatant Substances 0.000 description 2
229930182912 cyclosporin Natural products 0.000 description 2
230000002559 cytogenic effect Effects 0.000 description 2
230000003436 cytoskeletal effect Effects 0.000 description 2
230000007547 defect Effects 0.000 description 2
208000016097 disease of metabolism Diseases 0.000 description 2
235000021186 dishes Nutrition 0.000 description 2
238000012137 double-staining Methods 0.000 description 2
210000005069 ears Anatomy 0.000 description 2
108010048367 enhanced green fluorescent protein Proteins 0.000 description 2
210000002907 exocrine cell Anatomy 0.000 description 2
239000012091 fetal bovine serum Substances 0.000 description 2
210000003953 foreskin Anatomy 0.000 description 2
210000003976 gap junction Anatomy 0.000 description 2
230000002496 gastric effect Effects 0.000 description 2
230000007045 gastrulation Effects 0.000 description 2
101150003286 gata4 gene Proteins 0.000 description 2
210000004907 gland Anatomy 0.000 description 2
RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
210000003780 hair follicle Anatomy 0.000 description 2
208000006454 hepatitis Diseases 0.000 description 2
231100000283 hepatitis Toxicity 0.000 description 2
230000001744 histochemical effect Effects 0.000 description 2
238000009396 hybridization Methods 0.000 description 2
230000001146 hypoxic effect Effects 0.000 description 2
230000006058 immune tolerance Effects 0.000 description 2
238000013394 immunophenotyping Methods 0.000 description 2
230000001506 immunosuppresive effect Effects 0.000 description 2
230000007574 infarction Effects 0.000 description 2
239000012678 infectious agent Substances 0.000 description 2
210000002490 intestinal epithelial cell Anatomy 0.000 description 2
238000010253 intravenous injection Methods 0.000 description 2
210000004153 islets of langerhan Anatomy 0.000 description 2
238000002372 labelling Methods 0.000 description 2
210000003140 lateral ventricle Anatomy 0.000 description 2
239000003446 ligand Substances 0.000 description 2
210000000982 limb bud Anatomy 0.000 description 2
208000007903 liver failure Diseases 0.000 description 2
231100000835 liver failure Toxicity 0.000 description 2
208000002780 macular degeneration Diseases 0.000 description 2
238000012423 maintenance Methods 0.000 description 2
238000005259 measurement Methods 0.000 description 2
210000003593 megakaryocyte Anatomy 0.000 description 2
210000001704 mesoblast Anatomy 0.000 description 2
230000004060 metabolic process Effects 0.000 description 2
229920000609 methyl cellulose Polymers 0.000 description 2
239000001923 methylcellulose Substances 0.000 description 2
239000011325 microbead Substances 0.000 description 2
210000003657 middle cerebral artery Anatomy 0.000 description 2
239000003068 molecular probe Substances 0.000 description 2
238000004264 monolayer culture Methods 0.000 description 2
230000035772 mutation Effects 0.000 description 2
210000005155 neural progenitor cell Anatomy 0.000 description 2
230000000926 neurological effect Effects 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
210000000633 nuclear envelope Anatomy 0.000 description 2
210000000956 olfactory bulb Anatomy 0.000 description 2
230000003287 optical effect Effects 0.000 description 2
201000008482 osteoarthritis Diseases 0.000 description 2
244000045947 parasite Species 0.000 description 2
230000001936 parietal effect Effects 0.000 description 2
244000052769 pathogen Species 0.000 description 2
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 2
210000005259 peripheral blood Anatomy 0.000 description 2
239000011886 peripheral blood Substances 0.000 description 2
108040007629 peroxidase activity proteins Proteins 0.000 description 2
239000013612 plasmid Substances 0.000 description 2
238000002203 pretreatment Methods 0.000 description 2
230000000750 progressive effect Effects 0.000 description 2
230000002062 proliferating effect Effects 0.000 description 2
230000035755 proliferation Effects 0.000 description 2
AAEVYOVXGOFMJO-UHFFFAOYSA-N prometryn Chemical compound CSC1=NC(NC(C)C)=NC(NC(C)C)=N1 AAEVYOVXGOFMJO-UHFFFAOYSA-N 0.000 description 2
210000004129 prosencephalon Anatomy 0.000 description 2
229940048914 protamine Drugs 0.000 description 2
230000001681 protective effect Effects 0.000 description 2
230000005855 radiation Effects 0.000 description 2
HSSLDCABUXLXKM-UHFFFAOYSA-N resorufin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3N=C21 HSSLDCABUXLXKM-UHFFFAOYSA-N 0.000 description 2
229930002330 retinoic acid Natural products 0.000 description 2
229960004586 rosiglitazone Drugs 0.000 description 2
210000004761 scalp Anatomy 0.000 description 2
230000009758 senescence Effects 0.000 description 2
239000004017 serum-free culture medium Substances 0.000 description 2
210000002363 skeletal muscle cell Anatomy 0.000 description 2
210000002356 skeleton Anatomy 0.000 description 2
210000003625 skull Anatomy 0.000 description 2
MFBOGIVSZKQAPD-UHFFFAOYSA-M sodium butyrate Chemical compound [Na+].CCCC([O-])=O MFBOGIVSZKQAPD-UHFFFAOYSA-M 0.000 description 2
239000007787 solid Substances 0.000 description 2
238000010561 standard procedure Methods 0.000 description 2
239000000725 suspension Substances 0.000 description 2
210000002435 tendon Anatomy 0.000 description 2
229940124597 therapeutic agent Drugs 0.000 description 2
210000001541 thymus gland Anatomy 0.000 description 2
201000002341 thymus lymphoma Diseases 0.000 description 2
210000002303 tibia Anatomy 0.000 description 2
229950003937 tolonium Drugs 0.000 description 2
HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 2
230000001988 toxicity Effects 0.000 description 2
231100000419 toxicity Toxicity 0.000 description 2
229960001727 tretinoin Drugs 0.000 description 2
210000000689 upper leg Anatomy 0.000 description 2
210000003932 urinary bladder Anatomy 0.000 description 2
210000004291 uterus Anatomy 0.000 description 2
230000007998 vessel formation Effects 0.000 description 2
VUDQSRFCCHQIIU-UHFFFAOYSA-N 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one Chemical compound CCCCCC(=O)C1=C(O)C(Cl)=C(OC)C(Cl)=C1O VUDQSRFCCHQIIU-UHFFFAOYSA-N 0.000 description 1
SGTNSNPWRIOYBX-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile Chemical compound C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 SGTNSNPWRIOYBX-UHFFFAOYSA-N 0.000 description 1
UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
101150096316 5 gene Proteins 0.000 description 1
LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical compound C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 description 1
BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 1
ZPSOKQFFOYYPKC-UHFFFAOYSA-N 7-pentoxyphenoxazin-3-one Chemical compound C1=CC(=O)C=C2OC3=CC(OCCCCC)=CC=C3N=C21 ZPSOKQFFOYYPKC-UHFFFAOYSA-N 0.000 description 1
102100031585 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Human genes 0.000 description 1
101710129138 ATP synthase subunit 9, mitochondrial Proteins 0.000 description 1
101710168506 ATP synthase subunit C, plastid Proteins 0.000 description 1
101710114069 ATP synthase subunit c Proteins 0.000 description 1
101710197943 ATP synthase subunit c, chloroplastic Proteins 0.000 description 1
101710187091 ATP synthase subunit c, sodium ion specific Proteins 0.000 description 1
101150084229 ATXN1 gene Proteins 0.000 description 1
102100022142 Achaete-scute homolog 1 Human genes 0.000 description 1
101710165190 Achaete-scute homolog 1 Proteins 0.000 description 1
108010059616 Activins Proteins 0.000 description 1
208000024827 Alzheimer disease Diseases 0.000 description 1
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
101100059544 Arabidopsis thaliana CDC5 gene Proteins 0.000 description 1
101100152731 Arabidopsis thaliana TH2 gene Proteins 0.000 description 1
201000001320 Atherosclerosis Diseases 0.000 description 1
208000003950 B-cell lymphoma Diseases 0.000 description 1
208000035143 Bacterial infection Diseases 0.000 description 1
102000015735 Beta-catenin Human genes 0.000 description 1
108060000903 Beta-catenin Proteins 0.000 description 1
102100026189 Beta-galactosidase Human genes 0.000 description 1
201000004569 Blindness Diseases 0.000 description 1
208000010392 Bone Fractures Diseases 0.000 description 1
108090000654 Bone morphogenetic protein 1 Proteins 0.000 description 1
102000004152 Bone morphogenetic protein 1 Human genes 0.000 description 1
102100022526 Bone morphogenetic protein 5 Human genes 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
208000014644 Brain disease Diseases 0.000 description 1
206010006187 Breast cancer Diseases 0.000 description 1
208000026310 Breast neoplasm Diseases 0.000 description 1
102100021943 C-C motif chemokine 2 Human genes 0.000 description 1
101100497957 Caenorhabditis elegans cyn-2 gene Proteins 0.000 description 1
101100257372 Caenorhabditis elegans sox-3 gene Proteins 0.000 description 1
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
208000005623 Carcinogenesis Diseases 0.000 description 1
201000009030 Carcinoma Diseases 0.000 description 1
208000031229 Cardiomyopathies Diseases 0.000 description 1
102000053642 Catalytic RNA Human genes 0.000 description 1
108090000994 Catalytic RNA Proteins 0.000 description 1
102000000844 Cell Surface Receptors Human genes 0.000 description 1
108010001857 Cell Surface Receptors Proteins 0.000 description 1
208000018380 Chemical injury Diseases 0.000 description 1
108010012236 Chemokines Proteins 0.000 description 1
102000019034 Chemokines Human genes 0.000 description 1
108010009685 Cholinergic Receptors Proteins 0.000 description 1
241000252203 Clupea harengus Species 0.000 description 1
102000029816 Collagenase Human genes 0.000 description 1
108060005980 Collagenase Proteins 0.000 description 1
206010009944 Colon cancer Diseases 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
102000010970 Connexin Human genes 0.000 description 1
108050001175 Connexin Proteins 0.000 description 1
208000003407 Creutzfeldt-Jakob Syndrome Diseases 0.000 description 1
101710104798 Cytochrome P450 3A2 Proteins 0.000 description 1
101100350574 Danio rerio otx1b gene Proteins 0.000 description 1
206010012289 Dementia Diseases 0.000 description 1
108010008532 Deoxyribonuclease I Proteins 0.000 description 1
102100030012 Deoxyribonuclease-1 Human genes 0.000 description 1
206010012689 Diabetic retinopathy Diseases 0.000 description 1
102100024746 Dihydrofolate reductase Human genes 0.000 description 1
102000001039 Dystrophin Human genes 0.000 description 1
108010069091 Dystrophin Proteins 0.000 description 1
108010024212 E-Selectin Proteins 0.000 description 1
238000008157 ELISA kit Methods 0.000 description 1
101150002621 EPO gene Proteins 0.000 description 1
208000016952 Ear injury Diseases 0.000 description 1
102100038591 Endothelial cell-selective adhesion molecule Human genes 0.000 description 1
108010043939 Ephrin-A5 Proteins 0.000 description 1
102100033941 Ephrin-A5 Human genes 0.000 description 1
YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
101000669929 Escherichia coli (strain K12) 50S ribosomal protein L16 3-hydroxylase Proteins 0.000 description 1
101000799969 Escherichia coli (strain K12) Alpha-2-macroglobulin Proteins 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
208000007241 Experimental Diabetes Mellitus Diseases 0.000 description 1
206010016654 Fibrosis Diseases 0.000 description 1
102100020715 Fms-related tyrosine kinase 3 ligand protein Human genes 0.000 description 1
101710162577 Fms-related tyrosine kinase 3 ligand protein Proteins 0.000 description 1
206010017076 Fracture Diseases 0.000 description 1
206010017533 Fungal infection Diseases 0.000 description 1
210000000712 G cell Anatomy 0.000 description 1
102000005915 GABA Receptors Human genes 0.000 description 1
108010005551 GABA Receptors Proteins 0.000 description 1
102000004300 GABA-A Receptors Human genes 0.000 description 1
108090000839 GABA-A Receptors Proteins 0.000 description 1
101710082961 GATA-binding factor 2 Proteins 0.000 description 1
108010052320 GATA4 Transcription Factor Proteins 0.000 description 1
102000018715 GATA4 Transcription Factor Human genes 0.000 description 1
108020004206 Gamma-glutamyltransferase Proteins 0.000 description 1
208000015872 Gaucher disease Diseases 0.000 description 1
208000010412 Glaucoma Diseases 0.000 description 1
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
108010024636 Glutathione Proteins 0.000 description 1
102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
102000010726 Glycine Plasma Membrane Transport Proteins Human genes 0.000 description 1
108010063380 Glycine Plasma Membrane Transport Proteins Proteins 0.000 description 1
102000011714 Glycine Receptors Human genes 0.000 description 1
108010076533 Glycine Receptors Proteins 0.000 description 1
102000028180 Glycophorins Human genes 0.000 description 1
102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 1
108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
102100028967 HLA class I histocompatibility antigen, alpha chain G Human genes 0.000 description 1
101710197836 HLA class I histocompatibility antigen, alpha chain G Proteins 0.000 description 1
101000861138 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Probable ribosomal oxygenase HI_0396 Proteins 0.000 description 1
206010019280 Heart failures Diseases 0.000 description 1
208000032843 Hemorrhage Diseases 0.000 description 1
241000893570 Hendra henipavirus Species 0.000 description 1
102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 1
108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 1
108010027412 Histocompatibility Antigens Class II Proteins 0.000 description 1
102000018713 Histocompatibility Antigens Class II Human genes 0.000 description 1
101000777636 Homo sapiens ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Proteins 0.000 description 1
101000779615 Homo sapiens ALX homeobox protein 1 Proteins 0.000 description 1
101000937544 Homo sapiens Beta-2-microglobulin Proteins 0.000 description 1
101000899388 Homo sapiens Bone morphogenetic protein 5 Proteins 0.000 description 1
101000882622 Homo sapiens Endothelial cell-selective adhesion molecule Proteins 0.000 description 1
101000994378 Homo sapiens Integrin alpha-3 Proteins 0.000 description 1
101000994375 Homo sapiens Integrin alpha-4 Proteins 0.000 description 1
101000942967 Homo sapiens Leukemia inhibitory factor Proteins 0.000 description 1
101000576320 Homo sapiens Max-binding protein MNT Proteins 0.000 description 1
101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 1
101000600434 Homo sapiens Putative uncharacterized protein encoded by MIR7-3HG Proteins 0.000 description 1
101000754924 Homo sapiens Ribosomal oxygenase 1 Proteins 0.000 description 1
101000754919 Homo sapiens Ribosomal oxygenase 2 Proteins 0.000 description 1
101000830933 Homo sapiens TNF receptor-associated factor 4 Proteins 0.000 description 1
101000713575 Homo sapiens Tubulin beta-3 chain Proteins 0.000 description 1
101000976622 Homo sapiens Zinc finger protein 42 homolog Proteins 0.000 description 1
208000023105 Huntington disease Diseases 0.000 description 1
108010013214 Hyaluronan Receptors Proteins 0.000 description 1
102000018866 Hyaluronan Receptors Human genes 0.000 description 1
206010020772 Hypertension Diseases 0.000 description 1
102000026633 IL6 Human genes 0.000 description 1
102100026818 Inhibin beta E chain Human genes 0.000 description 1
102100032819 Integrin alpha-3 Human genes 0.000 description 1
102100032818 Integrin alpha-4 Human genes 0.000 description 1
108010047852 Integrin alphaVbeta3 Proteins 0.000 description 1
102100020881 Interleukin-1 alpha Human genes 0.000 description 1
102100039064 Interleukin-3 Human genes 0.000 description 1
102100037795 Interleukin-6 receptor subunit beta Human genes 0.000 description 1
101710152369 Interleukin-6 receptor subunit beta Proteins 0.000 description 1
241000581650 Ivesia Species 0.000 description 1
101710183399 Keratin, type I cytoskeletal 19 Proteins 0.000 description 1
108010066327 Keratin-18 Proteins 0.000 description 1
101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 1
239000002211 L-ascorbic acid Substances 0.000 description 1
235000000069 L-ascorbic acid Nutrition 0.000 description 1
FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 description 1
102100032352 Leukemia inhibitory factor Human genes 0.000 description 1
108010013709 Leukocyte Common Antigens Proteins 0.000 description 1
102000017095 Leukocyte Common Antigens Human genes 0.000 description 1
208000006552 Lewis Lung Carcinoma Diseases 0.000 description 1
240000006240 Linum usitatissimum Species 0.000 description 1
235000004431 Linum usitatissimum Nutrition 0.000 description 1
102000004895 Lipoproteins Human genes 0.000 description 1
108090001030 Lipoproteins Proteins 0.000 description 1
206010025323 Lymphomas Diseases 0.000 description 1
101150115300 MAC1 gene Proteins 0.000 description 1
101150011585 MAT gene Proteins 0.000 description 1
101710091439 Major capsid protein 1 Proteins 0.000 description 1
102100025818 Major prion protein Human genes 0.000 description 1
102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 1
101710167839 Morphogenetic protein Proteins 0.000 description 1
206010068052 Mosaicism Diseases 0.000 description 1
208000002678 Mucopolysaccharidoses Diseases 0.000 description 1
208000034578 Multiple myelomas Diseases 0.000 description 1
241001529936 Murinae Species 0.000 description 1
241000699660 Mus musculus Species 0.000 description 1
101100446513 Mus musculus Fgf4 gene Proteins 0.000 description 1
101100350576 Mus musculus Otx1 gene Proteins 0.000 description 1
101100256746 Mus musculus Setdb1 gene Proteins 0.000 description 1
101100257376 Mus musculus Sox3 gene Proteins 0.000 description 1
101100099741 Mus musculus Tmprss11d gene Proteins 0.000 description 1
101000976618 Mus musculus Zinc finger protein 42 Proteins 0.000 description 1
208000031888 Mycoses Diseases 0.000 description 1
102100030741 Myelin protein P0 Human genes 0.000 description 1
108050003852 Myelin protein P0 Proteins 0.000 description 1
108010000123 Myelin-Oligodendrocyte Glycoprotein Proteins 0.000 description 1
102100023302 Myelin-oligodendrocyte glycoprotein Human genes 0.000 description 1
208000009525 Myocarditis Diseases 0.000 description 1
108020002144 NR4 subfamily Proteins 0.000 description 1
241001045988 Neogene Species 0.000 description 1
102100023057 Neurofilament light polypeptide Human genes 0.000 description 1
102100032063 Neurogenic differentiation factor 1 Human genes 0.000 description 1
108050000588 Neurogenic differentiation factor 1 Proteins 0.000 description 1
102100021852 Neuronal cell adhesion molecule Human genes 0.000 description 1
101710130688 Neuronal cell adhesion molecule Proteins 0.000 description 1
208000014060 Niemann-Pick disease Diseases 0.000 description 1
108010051791 Nuclear Antigens Proteins 0.000 description 1
102000019040 Nuclear Antigens Human genes 0.000 description 1
102000004264 Osteopontin Human genes 0.000 description 1
108010081689 Osteopontin Proteins 0.000 description 1
108010035766 P-Selectin Proteins 0.000 description 1
238000010222 PCR analysis Methods 0.000 description 1
101150023417 PPARG gene Proteins 0.000 description 1
241000609499 Palicourea Species 0.000 description 1
206010061902 Pancreatic neoplasm Diseases 0.000 description 1
241001494479 Pecora Species 0.000 description 1
206010035226 Plasma cell myeloma Diseases 0.000 description 1
102000010752 Plasminogen Inactivators Human genes 0.000 description 1
108010077971 Plasminogen Inactivators Proteins 0.000 description 1
102000029797 Prion Human genes 0.000 description 1
108091000054 Prion Proteins 0.000 description 1
206010060862 Prostate cancer Diseases 0.000 description 1
208000000236 Prostatic Neoplasms Diseases 0.000 description 1
102000052575 Proto-Oncogene Human genes 0.000 description 1
108700020978 Proto-Oncogene Proteins 0.000 description 1
102100037401 Putative uncharacterized protein encoded by MIR7-3HG Human genes 0.000 description 1
108010005730 R-SNARE Proteins Proteins 0.000 description 1
238000010240 RT-PCR analysis Methods 0.000 description 1
101000930457 Rattus norvegicus Albumin Proteins 0.000 description 1
208000017442 Retinal disease Diseases 0.000 description 1
206010038910 Retinitis Diseases 0.000 description 1
102100022091 Ribosomal oxygenase 1 Human genes 0.000 description 1
239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
101150020367 SOX11 gene Proteins 0.000 description 1
206010070834 Sensitisation Diseases 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
208000020339 Spinal injury Diseases 0.000 description 1
108010090804 Streptavidin Proteins 0.000 description 1
208000006011 Stroke Diseases 0.000 description 1
102000002215 Synaptobrevin Human genes 0.000 description 1
102100024809 TNF receptor-associated factor 4 Human genes 0.000 description 1
108010033711 Telomeric Repeat Binding Protein 1 Proteins 0.000 description 1
102100036497 Telomeric repeat-binding factor 1 Human genes 0.000 description 1
108010000499 Thromboplastin Proteins 0.000 description 1
208000037432 Thymic tumor Diseases 0.000 description 1
208000000728 Thymus Neoplasms Diseases 0.000 description 1
102100030859 Tissue factor Human genes 0.000 description 1
101001023030 Toxoplasma gondii Myosin-D Proteins 0.000 description 1
244000078912 Trichosanthes cucumerina Species 0.000 description 1
235000008322 Trichosanthes cucumerina Nutrition 0.000 description 1
102100036859 Troponin I, cardiac muscle Human genes 0.000 description 1
101710128251 Troponin I, cardiac muscle Proteins 0.000 description 1
102100036790 Tubulin beta-3 chain Human genes 0.000 description 1
206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
101100226904 Unknown prokaryotic organism FCS1 gene Proteins 0.000 description 1
108010046334 Urease Proteins 0.000 description 1
108010053099 Vascular Endothelial Growth Factor Receptor-2 Proteins 0.000 description 1
208000036142 Viral infection Diseases 0.000 description 1
238000011892 Von Kossa's method Methods 0.000 description 1
206010052428 Wound Diseases 0.000 description 1
238000010817 Wright-Giemsa staining Methods 0.000 description 1
102100023550 Zinc finger protein 42 homolog Human genes 0.000 description 1
IZOBIZVXEKNCNN-ZNQIEUMMSA-N [(1r,2r,3's,4e,5s)-4-hexa-2,4-diynylidenespiro[3,6-dioxabicyclo[3.1.0]hexane-2,6'-oxane]-3'-yl] 3-methylbutanoate Chemical compound CC#CC#C\C=C([C@H]1O[C@H]11)\O[C@@]21CC[C@H](OC(=O)CC(C)C)CO2 IZOBIZVXEKNCNN-ZNQIEUMMSA-N 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
230000007488 abnormal function Effects 0.000 description 1
230000035508 accumulation Effects 0.000 description 1
238000009825 accumulation Methods 0.000 description 1
OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
229960004373 acetylcholine Drugs 0.000 description 1
102000034337 acetylcholine receptors Human genes 0.000 description 1
239000002253 acid Substances 0.000 description 1
239000008186 active pharmaceutical agent Substances 0.000 description 1
239000000488 activin Substances 0.000 description 1
230000002411 adverse Effects 0.000 description 1
239000011717 all-trans-retinol Substances 0.000 description 1
235000019169 all-trans-retinol Nutrition 0.000 description 1
230000007815 allergy Effects 0.000 description 1
230000004075 alteration Effects 0.000 description 1
210000002821 alveolar epithelial cell Anatomy 0.000 description 1
230000019552 anatomical structure morphogenesis Effects 0.000 description 1
239000004037 angiogenesis inhibitor Substances 0.000 description 1
238000010171 animal model Methods 0.000 description 1
210000001557 animal structure Anatomy 0.000 description 1
230000001772 anti-angiogenic effect Effects 0.000 description 1
230000001093 anti-cancer Effects 0.000 description 1
230000003466 anti-cipated effect Effects 0.000 description 1
230000002482 anti-endothelial effect Effects 0.000 description 1
230000003409 anti-rejection Effects 0.000 description 1
230000000692 anti-sense effect Effects 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
230000005775 apoptotic pathway Effects 0.000 description 1
238000003491 array Methods 0.000 description 1
108010083526 asialo-von Willebrand Factor Proteins 0.000 description 1
230000003140 astrocytic effect Effects 0.000 description 1
230000003190 augmentative effect Effects 0.000 description 1
229940058140 avita Drugs 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
208000022362 bacterial infectious disease Diseases 0.000 description 1
HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
210000003445 biliary tract Anatomy 0.000 description 1
230000000975 bioactive effect Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
230000000740 bleeding effect Effects 0.000 description 1
239000010836 blood and blood product Substances 0.000 description 1
229940125691 blood product Drugs 0.000 description 1
239000005388 borosilicate glass Substances 0.000 description 1
238000009395 breeding Methods 0.000 description 1
230000001488 breeding effect Effects 0.000 description 1
210000000621 bronchi Anatomy 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000001506 calcium phosphate Substances 0.000 description 1
229910000389 calcium phosphate Inorganic materials 0.000 description 1
235000011010 calcium phosphates Nutrition 0.000 description 1
230000036952 cancer formation Effects 0.000 description 1
239000002775 capsule Substances 0.000 description 1
235000011089 carbon dioxide Nutrition 0.000 description 1
231100000504 carcinogenesis Toxicity 0.000 description 1
208000015100 cartilage disease Diseases 0.000 description 1
230000021164 cell adhesion Effects 0.000 description 1
239000006143 cell culture medium Substances 0.000 description 1
230000025084 cell cycle arrest Effects 0.000 description 1
230000011748 cell maturation Effects 0.000 description 1
238000002659 cell therapy Methods 0.000 description 1
230000003822 cell turnover Effects 0.000 description 1
230000017455 cell-cell adhesion Effects 0.000 description 1
230000019522 cellular metabolic process Effects 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
230000000973 chemotherapeutic effect Effects 0.000 description 1
229940044683 chemotherapy drug Drugs 0.000 description 1
RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 1
229960002327 chloral hydrate Drugs 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
210000002358 circulating endothelial cell Anatomy 0.000 description 1
230000007882 cirrhosis Effects 0.000 description 1
208000019425 cirrhosis of liver Diseases 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
230000004186 co-expression Effects 0.000 description 1
230000015271 coagulation Effects 0.000 description 1
238000005345 coagulation Methods 0.000 description 1
229960002424 collagenase Drugs 0.000 description 1
208000029742 colonic neoplasm Diseases 0.000 description 1
238000004737 colorimetric analysis Methods 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
239000002131 composite material Substances 0.000 description 1
230000001143 conditioned effect Effects 0.000 description 1
230000008602 contraction Effects 0.000 description 1
210000004087 cornea Anatomy 0.000 description 1
238000002316 cosmetic surgery Methods 0.000 description 1
238000012136 culture method Methods 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
102000003675 cytokine receptors Human genes 0.000 description 1
108010057085 cytokine receptors Proteins 0.000 description 1
210000004292 cytoskeleton Anatomy 0.000 description 1
229940127089 cytotoxic agent Drugs 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
230000007850 degeneration Effects 0.000 description 1
210000004443 dendritic cell Anatomy 0.000 description 1
238000000432 density-gradient centrifugation Methods 0.000 description 1
230000002999 depolarising effect Effects 0.000 description 1
210000004207 dermis Anatomy 0.000 description 1
235000005911 diet Nutrition 0.000 description 1
230000037213 diet Effects 0.000 description 1
230000001079 digestive effect Effects 0.000 description 1
108020001096 dihydrofolate reductase Proteins 0.000 description 1
208000035475 disorder Diseases 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
230000005593 dissociations Effects 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000009826 distribution Methods 0.000 description 1
241001493065 dsRNA viruses Species 0.000 description 1
230000004064 dysfunction Effects 0.000 description 1
230000002526 effect on cardiovascular system Effects 0.000 description 1
239000012636 effector Substances 0.000 description 1
230000007831 electrophysiology Effects 0.000 description 1
238000002001 electrophysiology Methods 0.000 description 1
210000002242 embryoid body Anatomy 0.000 description 1
230000002124 endocrine Effects 0.000 description 1
210000003890 endocrine cell Anatomy 0.000 description 1
210000002472 endoplasmic reticulum Anatomy 0.000 description 1
230000009764 endothelial cell sprouting Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
210000002514 epidermal stem cell Anatomy 0.000 description 1
210000002615 epidermis Anatomy 0.000 description 1
210000004920 epithelial cell of skin Anatomy 0.000 description 1
230000010437 erythropoiesis Effects 0.000 description 1
238000011124 ex vivo culture Methods 0.000 description 1
210000003722 extracellular fluid Anatomy 0.000 description 1
239000012894 fetal calf serum Substances 0.000 description 1
230000005714 functional activity Effects 0.000 description 1
102000054078 gamma Catenin Human genes 0.000 description 1
108010084448 gamma Catenin Proteins 0.000 description 1
102000006640 gamma-Glutamyltransferase Human genes 0.000 description 1
238000003500 gene array Methods 0.000 description 1
230000009395 genetic defect Effects 0.000 description 1
230000001434 glomerular Effects 0.000 description 1
230000004110 gluconeogenesis Effects 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
229960003180 glutathione Drugs 0.000 description 1
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
239000008187 granular material Substances 0.000 description 1
238000012835 hanging drop method Methods 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
210000003128 head Anatomy 0.000 description 1
230000035876 healing Effects 0.000 description 1
230000036541 health Effects 0.000 description 1
210000003709 heart valve Anatomy 0.000 description 1
210000000777 hematopoietic system Anatomy 0.000 description 1
230000000004 hemodynamic effect Effects 0.000 description 1
206010019692 hepatic necrosis Diseases 0.000 description 1
208000002672 hepatitis B Diseases 0.000 description 1
235000019514 herring Nutrition 0.000 description 1
238000000265 homogenisation Methods 0.000 description 1
102000047279 human B2M Human genes 0.000 description 1
230000028996 humoral immune response Effects 0.000 description 1
238000003384 imaging method Methods 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
208000026278 immune system disease Diseases 0.000 description 1
230000003053 immunization Effects 0.000 description 1
238000002649 immunization Methods 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
238000010820 immunofluorescence microscopy Methods 0.000 description 1
238000003125 immunofluorescent labeling Methods 0.000 description 1
238000011532 immunohistochemical staining Methods 0.000 description 1
238000013388 immunohistochemistry analysis Methods 0.000 description 1
229940124589 immunosuppressive drug Drugs 0.000 description 1
230000006872 improvement Effects 0.000 description 1
238000012625 in-situ measurement Methods 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
208000027866 inflammatory disease Diseases 0.000 description 1
230000002757 inflammatory effect Effects 0.000 description 1
238000013383 initial experiment Methods 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
230000010354 integration Effects 0.000 description 1
102000006495 integrins Human genes 0.000 description 1
108010044426 integrins Proteins 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000008611 intercellular interaction Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
210000002977 intracellular fluid Anatomy 0.000 description 1
230000000302 ischemic effect Effects 0.000 description 1
230000000366 juvenile effect Effects 0.000 description 1
210000002510 keratinocyte Anatomy 0.000 description 1
210000003292 kidney cell Anatomy 0.000 description 1
208000017169 kidney disease Diseases 0.000 description 1
210000001985 kidney epithelial cell Anatomy 0.000 description 1
230000002045 lasting effect Effects 0.000 description 1
231100000518 lethal Toxicity 0.000 description 1
230000001665 lethal effect Effects 0.000 description 1
238000012417 linear regression Methods 0.000 description 1
102000019758 lipid binding proteins Human genes 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000007788 liquid Substances 0.000 description 1
239000007791 liquid phase Substances 0.000 description 1
231100000149 liver necrosis Toxicity 0.000 description 1
210000003141 lower extremity Anatomy 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
210000002540 macrophage Anatomy 0.000 description 1
159000000003 magnesium salts Chemical class 0.000 description 1
239000006249 magnetic particle Substances 0.000 description 1
238000007885 magnetic separation Methods 0.000 description 1
230000036244 malformation Effects 0.000 description 1
230000003211 malignant effect Effects 0.000 description 1
210000005171 mammalian brain Anatomy 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
108700010762 mat Proteins 0.000 description 1
230000008774 maternal effect Effects 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
230000006371 metabolic abnormality Effects 0.000 description 1
230000007102 metabolic function Effects 0.000 description 1
238000010208 microarray analysis Methods 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
210000004925 microvascular endothelial cell Anatomy 0.000 description 1
230000022256 midbrain development Effects 0.000 description 1
230000005012 migration Effects 0.000 description 1
238000013508 migration Methods 0.000 description 1
239000007758 minimum essential medium Substances 0.000 description 1
230000004898 mitochondrial function Effects 0.000 description 1
238000000329 molecular dynamics simulation Methods 0.000 description 1
206010028093 mucopolysaccharidosis Diseases 0.000 description 1
210000005088 multinucleated cell Anatomy 0.000 description 1
210000000663 muscle cell Anatomy 0.000 description 1
210000003643 myeloid progenitor cell Anatomy 0.000 description 1
230000002107 myocardial effect Effects 0.000 description 1
208000010125 myocardial infarction Diseases 0.000 description 1
239000013642 negative control Substances 0.000 description 1
101150091879 neo gene Proteins 0.000 description 1
210000000653 nervous system Anatomy 0.000 description 1
238000007857 nested PCR Methods 0.000 description 1
230000004770 neurodegeneration Effects 0.000 description 1
208000015122 neurodegenerative disease Diseases 0.000 description 1
230000007472 neurodevelopment Effects 0.000 description 1
230000004766 neurogenesis Effects 0.000 description 1
230000009207 neuronal maturation Effects 0.000 description 1
210000000440 neutrophil Anatomy 0.000 description 1
239000002547 new drug Substances 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
201000002528 pancreatic cancer Diseases 0.000 description 1
230000009996 pancreatic endocrine effect Effects 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
210000003899 penis Anatomy 0.000 description 1
208000028169 periodontal disease Diseases 0.000 description 1
210000001428 peripheral nervous system Anatomy 0.000 description 1
230000008823 permeabilization Effects 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
239000002797 plasminogen activator inhibitor Substances 0.000 description 1
238000007747 plating Methods 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
239000013641 positive control Substances 0.000 description 1
230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 1
210000002996 primitive erythroblast Anatomy 0.000 description 1
238000012545 processing Methods 0.000 description 1
239000003805 procoagulant Substances 0.000 description 1
230000001737 promoting effect Effects 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
235000004252 protein component Nutrition 0.000 description 1
230000004853 protein function Effects 0.000 description 1
230000009145 protein modification Effects 0.000 description 1
230000005180 public health Effects 0.000 description 1
238000001959 radiotherapy Methods 0.000 description 1
238000011536 re-plating Methods 0.000 description 1
238000011084 recovery Methods 0.000 description 1
108010054624 red fluorescent protein Proteins 0.000 description 1
238000006722 reduction reaction Methods 0.000 description 1
230000001172 regenerating effect Effects 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
230000008844 regulatory mechanism Effects 0.000 description 1
230000008439 repair process Effects 0.000 description 1
238000009256 replacement therapy Methods 0.000 description 1
230000008672 reprogramming Effects 0.000 description 1
230000036390 resting membrane potential Effects 0.000 description 1
210000001525 retina Anatomy 0.000 description 1
230000002207 retinal effect Effects 0.000 description 1
238000003757 reverse transcription PCR Methods 0.000 description 1
210000001202 rhombencephalon Anatomy 0.000 description 1
108091092562 ribozyme Proteins 0.000 description 1
102220259319 rs1553651734 Human genes 0.000 description 1
235000015067 sauces Nutrition 0.000 description 1
230000007017 scission Effects 0.000 description 1
230000028327 secretion Effects 0.000 description 1
238000010187 selection method Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
230000008313 sensitization Effects 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
230000002295 serotoninergic effect Effects 0.000 description 1
208000007056 sickle cell anemia Diseases 0.000 description 1
230000005634 sigma model Effects 0.000 description 1
229910052709 silver Inorganic materials 0.000 description 1
239000004332 silver Substances 0.000 description 1
210000004683 skeletal myoblast Anatomy 0.000 description 1
150000003385 sodium Chemical class 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
159000000000 sodium salts Chemical class 0.000 description 1
210000004872 soft tissue Anatomy 0.000 description 1
230000000392 somatic effect Effects 0.000 description 1
201000003624 spinocerebellar ataxia type 1 Diseases 0.000 description 1
230000002269 spontaneous effect Effects 0.000 description 1
238000013222 sprague-dawley male rat Methods 0.000 description 1
238000007447 staining method Methods 0.000 description 1
150000003431 steroids Chemical class 0.000 description 1
230000004936 stimulating effect Effects 0.000 description 1
230000000638 stimulation Effects 0.000 description 1
210000002536 stromal cell Anatomy 0.000 description 1
239000012879 subculture medium Substances 0.000 description 1
238000011410 subtraction method Methods 0.000 description 1
229960005404 sulfamethoxazole Drugs 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
JLKIGFTWXXRPMT-UHFFFAOYSA-N sulphamethoxazole Chemical compound O1C(C)=CC(NS(=O)(=O)C=2C=CC(N)=CC=2)=N1 JLKIGFTWXXRPMT-UHFFFAOYSA-N 0.000 description 1
230000008093 supporting effect Effects 0.000 description 1
230000003319 supportive effect Effects 0.000 description 1
238000004114 suspension culture Methods 0.000 description 1
230000000946 synaptic effect Effects 0.000 description 1
210000003568 synaptosome Anatomy 0.000 description 1
238000007910 systemic administration Methods 0.000 description 1
230000008685 targeting Effects 0.000 description 1
102000013498 tau Proteins Human genes 0.000 description 1
108010026424 tau Proteins Proteins 0.000 description 1
CFMYXEVWODSLAX-QOZOJKKESA-N tetrodotoxin Chemical compound O([C@@]([C@H]1O)(O)O[C@H]2[C@@]3(O)CO)[C@H]3[C@@H](O)[C@]11[C@H]2[C@@H](O)N=C(N)N1 CFMYXEVWODSLAX-QOZOJKKESA-N 0.000 description 1
229950010357 tetrodotoxin Drugs 0.000 description 1
CFMYXEVWODSLAX-UHFFFAOYSA-N tetrodotoxin Natural products C12C(O)NC(=N)NC2(C2O)C(O)C3C(CO)(O)C1OC2(O)O3 CFMYXEVWODSLAX-UHFFFAOYSA-N 0.000 description 1
230000008719 thickening Effects 0.000 description 1
201000009377 thymus cancer Diseases 0.000 description 1
230000009772 tissue formation Effects 0.000 description 1
208000037816 tissue injury Diseases 0.000 description 1
239000003053 toxin Substances 0.000 description 1
231100000765 toxin Toxicity 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
230000002463 transducing effect Effects 0.000 description 1
238000001890 transfection Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1
238000011830 transgenic mouse model Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
230000032258 transport Effects 0.000 description 1
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 1
229960001082 trimethoprim Drugs 0.000 description 1
229960001641 troglitazone Drugs 0.000 description 1
GXPHKUHSUJUWKP-UHFFFAOYSA-N troglitazone Chemical compound C1CC=2C(C)=C(O)C(C)=C(C)C=2OC1(C)COC(C=C1)=CC=C1CC1SC(=O)NC1=O GXPHKUHSUJUWKP-UHFFFAOYSA-N 0.000 description 1
GXPHKUHSUJUWKP-NTKDMRAZSA-N troglitazone Natural products C([C@@]1(OC=2C(C)=C(C(=C(C)C=2CC1)O)C)C)OC(C=C1)=CC=C1C[C@H]1SC(=O)NC1=O GXPHKUHSUJUWKP-NTKDMRAZSA-N 0.000 description 1
210000005239 tubule Anatomy 0.000 description 1
201000011296 tyrosinemia Diseases 0.000 description 1
201000007972 tyrosinemia type I Diseases 0.000 description 1
210000001364 upper extremity Anatomy 0.000 description 1
210000002438 upper gastrointestinal tract Anatomy 0.000 description 1
210000003556 vascular endothelial cell Anatomy 0.000 description 1
230000002227 vasoactive effect Effects 0.000 description 1
239000003981 vehicle Substances 0.000 description 1
230000035899 viability Effects 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
108091005957 yellow fluorescent proteins Proteins 0.000 description 1

JP2002565063A 2001-02-14 2002-02-14 多能性成体幹細胞、その起源、それを得る方法および維持する方法、それを分化させる方法、その使用法、ならびにそれ由来の細胞 Withdrawn JP2004529621A (ja)