JP2004514459A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2004514459A5 JP2004514459A5 JP2002550090A JP2002550090A JP2004514459A5 JP 2004514459 A5 JP2004514459 A5 JP 2004514459A5 JP 2002550090 A JP2002550090 A JP 2002550090A JP 2002550090 A JP2002550090 A JP 2002550090A JP 2004514459 A5 JP2004514459 A5 JP 2004514459A5
- Authority
- JP
- Japan
- Prior art keywords
- protease
- hcv
- cleavage
- group
- self
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 claims 29
- 239000004365 Protease Substances 0.000 claims 29
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 29
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims 6
- 238000003776 cleavage reaction Methods 0.000 claims 6
- 238000000034 method Methods 0.000 claims 6
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 150000001413 amino acids Chemical class 0.000 claims 4
- 239000012634 fragment Substances 0.000 claims 4
- 229960004198 guanidine Drugs 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 230000003196 chaotropic effect Effects 0.000 claims 3
- 239000003112 inhibitor Substances 0.000 claims 3
- 230000007017 scission Effects 0.000 claims 3
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 claims 2
- 125000000729 N-terminal amino-acid group Chemical group 0.000 claims 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 2
- 239000000654 additive Substances 0.000 claims 2
- 230000000996 additive effect Effects 0.000 claims 2
- 229940024606 amino acid Drugs 0.000 claims 2
- 239000004202 carbamide Substances 0.000 claims 2
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000004094 surface-active agent Substances 0.000 claims 2
- 229940045136 urea Drugs 0.000 claims 2
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 claims 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims 1
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims 1
- 239000013504 Triton X-100 Substances 0.000 claims 1
- 229920004890 Triton X-100 Polymers 0.000 claims 1
- 229960003589 arginine hydrochloride Drugs 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- NLEBIOOXCVAHBD-QKMCSOCLSA-N dodecyl beta-D-maltoside Chemical compound O[C@@H]1[C@@H](O)[C@H](OCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 NLEBIOOXCVAHBD-QKMCSOCLSA-N 0.000 claims 1
- 239000000693 micelle Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- OGGIFKYAUCDPFX-UHFFFAOYSA-N n,n-diethyldodecan-1-amine oxide Chemical compound CCCCCCCCCCCC[N+]([O-])(CC)CC OGGIFKYAUCDPFX-UHFFFAOYSA-N 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25603100P | 2000-12-15 | 2000-12-15 | |
| PCT/CA2001/001796 WO2002048375A2 (en) | 2000-12-15 | 2001-12-13 | Purified active hcv ns2/3 protease |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004514459A JP2004514459A (ja) | 2004-05-20 |
| JP2004514459A5 true JP2004514459A5 (https=) | 2005-06-23 |
| JP3878132B2 JP3878132B2 (ja) | 2007-02-07 |
Family
ID=22970839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002550090A Expired - Fee Related JP3878132B2 (ja) | 2000-12-15 | 2001-12-13 | 精製活性型ｈｃｖｎｓ２／３プロテアーゼ |
Country Status (13)
| Country | Link |
|---|---|
| US (4) | US6815159B2 (https=) |
| EP (1) | EP1343897B1 (https=) |
| JP (1) | JP3878132B2 (https=) |
| AT (1) | ATE356875T1 (https=) |
| AU (1) | AU2468802A (https=) |
| CA (1) | CA2430661C (https=) |
| DE (1) | DE60127299T2 (https=) |
| ES (1) | ES2282320T3 (https=) |
| HU (1) | HUP0302558A3 (https=) |
| IL (2) | IL155760A0 (https=) |
| MX (1) | MXPA03004835A (https=) |
| NZ (1) | NZ526863A (https=) |
| WO (1) | WO2002048375A2 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0006537D0 (en) * | 2000-03-17 | 2000-05-10 | Angeletti P Ist Richerche Bio | Assays and screening methods |
| MXPA03004835A (es) * | 2000-12-15 | 2005-04-19 | Boehringer Ingelheim Ltd | Proteasa ns2/3 activa, purificada de virus de hepatitis c. |
| DE10105912A1 (de) * | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Rekombinante Proteinase K |
| US7785875B2 (en) * | 2004-07-03 | 2010-08-31 | Mogam Biotechnology Research Institute | Polynucleotide encoding HCV epitopes which can bind to various HLA supertypes, immunogenic composition comprising same and method of inducing an HCV-specific immune response using same |
| CA2627247C (en) * | 2005-10-28 | 2013-04-02 | Boehringer Ingelheim International Gmbh | Hepatitis c virus ns2/3 activity assay |
| US20070141701A1 (en) * | 2005-12-21 | 2007-06-21 | Boehringer Ingelheim International Gmbh | Hepatitis c virus ns2/3 assay |
| CN116874555B (zh) * | 2023-09-08 | 2023-11-28 | 成都华任康生物科技有限公司 | 一种置换液及其试剂盒和相关应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714371A (en) * | 1995-05-12 | 1998-02-03 | Schering Corporation | Method for refolding insoluble aggregates of hepatitis C virus protease |
| IT1277914B1 (it) * | 1995-08-22 | 1997-11-12 | Angeletti P Ist Richerche Bio | Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e |
| WO1999007733A2 (en) | 1997-08-11 | 1999-02-18 | Boehringer Ingelheim (Canada) Ltd. | Hepatitis c inhibitor peptides |
| US6280940B1 (en) | 1998-08-05 | 2001-08-28 | Agouron Pharmaceuticals, Inc. | Reporter gene system for use in cell-based assessment of inhibitors of the Hepatitis C virus protease |
| WO2001016379A1 (en) | 1999-08-30 | 2001-03-08 | Merck & Co., Inc. | Hepatitis c virus replication inhibitors |
| GB0006537D0 (en) * | 2000-03-17 | 2000-05-10 | Angeletti P Ist Richerche Bio | Assays and screening methods |
| MXPA03004835A (es) * | 2000-12-15 | 2005-04-19 | Boehringer Ingelheim Ltd | Proteasa ns2/3 activa, purificada de virus de hepatitis c. |
-
2001
- 2001-12-13 MX MXPA03004835A patent/MXPA03004835A/es active IP Right Grant
- 2001-12-13 EP EP01994573A patent/EP1343897B1/en not_active Expired - Lifetime
- 2001-12-13 IL IL15576001A patent/IL155760A0/xx active IP Right Grant
- 2001-12-13 AT AT01994573T patent/ATE356875T1/de active
- 2001-12-13 JP JP2002550090A patent/JP3878132B2/ja not_active Expired - Fee Related
- 2001-12-13 HU HU0302558A patent/HUP0302558A3/hu unknown
- 2001-12-13 AU AU2468802A patent/AU2468802A/xx not_active Withdrawn
- 2001-12-13 DE DE60127299T patent/DE60127299T2/de not_active Expired - Lifetime
- 2001-12-13 NZ NZ526863A patent/NZ526863A/en not_active IP Right Cessation
- 2001-12-13 WO PCT/CA2001/001796 patent/WO2002048375A2/en not_active Ceased
- 2001-12-13 CA CA2430661A patent/CA2430661C/en not_active Expired - Fee Related
- 2001-12-13 ES ES01994573T patent/ES2282320T3/es not_active Expired - Lifetime
- 2001-12-14 US US10/017,736 patent/US6815159B2/en not_active Expired - Lifetime
-
2003
- 2003-05-05 IL IL155760A patent/IL155760A/en not_active IP Right Cessation
- 2003-08-28 US US10/650,585 patent/US7264811B2/en not_active Expired - Lifetime
-
2005
- 2005-09-09 US US11/223,638 patent/US20070172936A1/en not_active Abandoned
-
2006
- 2006-12-12 US US11/609,635 patent/US20070160629A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Harris et al. | Interaction of poliovirus polypeptide 3CDpro with the 5 ‘and 3 ‘termini of the poliovirus genome. Identification of viral and cellular cofactors needed for efficient binding. | |
| Adachi et al. | Hemocyte components in crustaceans convert hemocyanin into a phenoloxidase-like enzyme | |
| Kouretova et al. | Effects of NS2B-NS3 protease and furin inhibition on West Nile and Dengue virus replication | |
| Gallinari et al. | Modulation of hepatitis C virus NS3 protease and helicase activities through the interaction with NS4A | |
| JP2004514459A5 (https=) | ||
| Böhm et al. | Novel insights into structure and function of factor XIIIa-inhibitor tridegin | |
| JP2008537482A5 (https=) | ||
| DE69031271D1 (de) | Varianten von pai-2 | |
| CN113521286B (zh) | 冠状病毒蛋白酶抑制剂及其应用 | |
| CA2430661A1 (en) | Purified active hcv ns2/3 protease | |
| Hesson et al. | Probing the Relationship between RNA-Stimulated ATPase and Helicase Activities of HCV NS3 Using 2 ‘-O-Methyl RNA Substrates | |
| Sekiya et al. | Structure/function analysis of an RNA aptamer for hepatitis C virus NS3 protease | |
| Kakiuchi et al. | Cleavage activity of hepatitis C virus serine proteinase | |
| Lorentsen et al. | Expression, refolding, and purification of recombinant human granzyme B | |
| Barathy et al. | Crystal structure of a putative aspartic proteinase domain of the Mycobacterium tuberculosis cell surface antigen PE_PGRS16 | |
| Yu et al. | Intein-mediated rapid purification and characterization of a novel recombinant agonist for VPAC2 | |
| JP2009513113A5 (https=) | ||
| Joy et al. | Biochemical characterisation of Murray Valley encephalitis virus proteinase | |
| Brown et al. | Identification of a region of hantavirus nucleocapsid protein required for RNA chaperone activity | |
| JP2009513113A (ja) | Ｃ型肝炎ウイルスｎｓ２／３活性のアッセイ法 | |
| Fynbo et al. | Characterization of a recombinant granzyme B derivative as a “restriction” protease | |
| Rothan et al. | Research Article Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells | |
| Dunn | Feline immunodeficiency virus retropepsin | |
| Kang et al. | Heterologous Expression and Purification of Anglerfish Somatostatin Precursors in Escherichia coli | |
| JPH099961A (ja) | 組換え型ｃ型肝炎ウイルスプロテア−ゼ |