NZ526863A - Method of producing a refolded inactive HCV NS2/3 protease - Google Patents
Method of producing a refolded inactive HCV NS2/3 proteaseInfo
- Publication number
- NZ526863A NZ526863A NZ526863A NZ52686301A NZ526863A NZ 526863 A NZ526863 A NZ 526863A NZ 526863 A NZ526863 A NZ 526863A NZ 52686301 A NZ52686301 A NZ 52686301A NZ 526863 A NZ526863 A NZ 526863A
- Authority
- NZ
- New Zealand
- Prior art keywords
- protease
- amino acid
- cleavage
- hcv
- final concentration
- Prior art date
Links
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- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (2)
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| US25603100P | 2000-12-15 | 2000-12-15 | |
| PCT/CA2001/001796 WO2002048375A2 (en) | 2000-12-15 | 2001-12-13 | Purified active hcv ns2/3 protease |
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| NZ526863A true NZ526863A (en) | 2006-06-30 |
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| NZ526863A NZ526863A (en) | 2000-12-15 | 2001-12-13 | Method of producing a refolded inactive HCV NS2/3 protease |
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| US (4) | US6815159B2 (https=) |
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| MX (1) | MXPA03004835A (https=) |
| NZ (1) | NZ526863A (https=) |
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| MXPA03004835A (es) * | 2000-12-15 | 2005-04-19 | Boehringer Ingelheim Ltd | Proteasa ns2/3 activa, purificada de virus de hepatitis c. |
| DE10105912A1 (de) * | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Rekombinante Proteinase K |
| US7785875B2 (en) * | 2004-07-03 | 2010-08-31 | Mogam Biotechnology Research Institute | Polynucleotide encoding HCV epitopes which can bind to various HLA supertypes, immunogenic composition comprising same and method of inducing an HCV-specific immune response using same |
| CA2627247C (en) * | 2005-10-28 | 2013-04-02 | Boehringer Ingelheim International Gmbh | Hepatitis c virus ns2/3 activity assay |
| US20070141701A1 (en) * | 2005-12-21 | 2007-06-21 | Boehringer Ingelheim International Gmbh | Hepatitis c virus ns2/3 assay |
| CN116874555B (zh) * | 2023-09-08 | 2023-11-28 | 成都华任康生物科技有限公司 | 一种置换液及其试剂盒和相关应用 |
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| US5714371A (en) * | 1995-05-12 | 1998-02-03 | Schering Corporation | Method for refolding insoluble aggregates of hepatitis C virus protease |
| IT1277914B1 (it) * | 1995-08-22 | 1997-11-12 | Angeletti P Ist Richerche Bio | Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e |
| WO1999007733A2 (en) | 1997-08-11 | 1999-02-18 | Boehringer Ingelheim (Canada) Ltd. | Hepatitis c inhibitor peptides |
| US6280940B1 (en) | 1998-08-05 | 2001-08-28 | Agouron Pharmaceuticals, Inc. | Reporter gene system for use in cell-based assessment of inhibitors of the Hepatitis C virus protease |
| WO2001016379A1 (en) | 1999-08-30 | 2001-03-08 | Merck & Co., Inc. | Hepatitis c virus replication inhibitors |
| GB0006537D0 (en) * | 2000-03-17 | 2000-05-10 | Angeletti P Ist Richerche Bio | Assays and screening methods |
| MXPA03004835A (es) * | 2000-12-15 | 2005-04-19 | Boehringer Ingelheim Ltd | Proteasa ns2/3 activa, purificada de virus de hepatitis c. |
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2001
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- 2001-12-13 EP EP01994573A patent/EP1343897B1/en not_active Expired - Lifetime
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- 2001-12-13 AT AT01994573T patent/ATE356875T1/de active
- 2001-12-13 JP JP2002550090A patent/JP3878132B2/ja not_active Expired - Fee Related
- 2001-12-13 HU HU0302558A patent/HUP0302558A3/hu unknown
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| MXPA03004835A (es) | 2005-04-19 |
| WO2002048375A3 (en) | 2003-02-27 |
| CA2430661C (en) | 2010-10-19 |
| JP2004514459A (ja) | 2004-05-20 |
| IL155760A0 (en) | 2003-12-23 |
| AU2468802A (en) | 2002-06-24 |
| ES2282320T3 (es) | 2007-10-16 |
| CA2430661A1 (en) | 2002-06-20 |
| US20070160629A1 (en) | 2007-07-12 |
| ATE356875T1 (de) | 2007-04-15 |
| HUP0302558A3 (en) | 2012-03-28 |
| US20070172936A1 (en) | 2007-07-26 |
| JP3878132B2 (ja) | 2007-02-07 |
| US6815159B2 (en) | 2004-11-09 |
| US20040077066A1 (en) | 2004-04-22 |
| DE60127299D1 (de) | 2007-04-26 |
| US7264811B2 (en) | 2007-09-04 |
| IL155760A (en) | 2009-02-11 |
| HUP0302558A2 (hu) | 2003-10-28 |
| DE60127299T2 (de) | 2007-08-30 |
| EP1343897A2 (en) | 2003-09-17 |
| WO2002048375A2 (en) | 2002-06-20 |
| EP1343897B1 (en) | 2007-03-14 |
| US20020192640A1 (en) | 2002-12-19 |
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