JP2004113249A - ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 - Google Patents
ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 Download PDFInfo
- Publication number
- JP2004113249A JP2004113249A JP2003383755A JP2003383755A JP2004113249A JP 2004113249 A JP2004113249 A JP 2004113249A JP 2003383755 A JP2003383755 A JP 2003383755A JP 2003383755 A JP2003383755 A JP 2003383755A JP 2004113249 A JP2004113249 A JP 2004113249A
- Authority
- JP
- Japan
- Prior art keywords
- der
- vii
- peptide
- nucleic acid
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43531—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from mites
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/7051—T-cell receptor (TcR)-CD3 complex
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/24—Immunology or allergic disorders
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/868—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, or immunotolerance
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Insects & Arthropods (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Pulmonology (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Peptides Or Proteins (AREA)
Abstract
【解決手段】 Dermatophagoides farinaet のアレルゲンであるDer f VIIの生物学的活性を少くとも1つ有するペプチドをコードする核酸分離物に係る発明を提供する。
【選択図】 図1
Description
図面の簡単な説明
図1はアレルギー性血清中のIgEのλgt11−HD6プラークとの結合頻度を示す。
Commonwealth Serum Laboratories,Parkville,Australia(Thomas,W.他,Int Arch Allergy Appl Immunol(1988) 85:127-9)から購入した生きた成体Dermatophagoides pteronyssinusからλgt11cDNAライブラリーを用意した。ライブラリーは、YoungとDavis(Proc.Natl.Acad.Sci.USA(1983)80:1194-1198)及びGublerとHoffman(Gene(1983)25:263-299)の方法に基づくChua他(J.Exp.Med(1988)167:175-182)に従って用意した。ポリアデニル化mRNAはD.pteronyssinus培養物から分離され、cDNAはキット(Amersham International,Bucks)を用いてリボヌクレアーゼH法により合成した(GulberとHoffman,前述)。EcoRIリンカ−を付加した後、cDNAをλgt11内に連結し、5×105個の組換え体のライブラリーを得る為にE.coliY1090(r-)(Promega Biotec,Madison,Wisconsin)内で培養した。
クローンHD6の812塩基対挿入はM13ベクターmp18及びmp19(Messing,Methods Enzymology(1983)101:20参照)でクローンされ、−40、一般プライマー及び内在プライマー(Messing前述)を用いて双方向の配列決定が行われた。ジデオキシヌクレオチド配列決定法(Sambrook,J.他Molecular Cloning.A Laboratory Manual.2nd Edition.Cold Spring Harbor:Cold Spring Harbor Laboratory Press,1989)が、32P−dATPおよびBiorad Sequi−gen電気泳動器と共にシーケナーゼ2.0キット(IBI,New Heaven,USA)を用いて行われた。一般及び内在プライマーを用いた配列分析に続いては、Der p VIIcDNA配列に基づく三つのプライマーが産生され、配列決定が行われた。プライマーの配列は以下の通りである。(1)GATCCAATTCACTATGAT(図3SEQ ID NO:4における塩基119−136);(2)GGTGAATTAGACATGCG(図3SEQ ID NO:3における塩基272−288);(3)TCAATTTTGGATCCAATTTTCGCT(SEQ ID NO:5における塩基584−607)。
λgt11 cDNAライブラリーを、CommonwealthSerum Laboratories,Parkville Australia(Thomas,W.他 Int Arch Allergy Appl Immunol(1988)85:127-9)より購入した生きたDermatophagoides farinae成体を用いて準備した。ライブラリーはTrudinger他((1991)Chem.Exp.Allergy,21:33-37)に従って用意した。
シーケナーゼ2.0版(USB Corp.,Cleveland,U.S.A.)を用いてその提供者の手順に従ってジデオキシヌクレオチドチェインターミネーター法によりDNA配列決定を行った。配列決定に用いられるプライマーはM13配列決定プライマー(−40)、17−mer GTTTTCCCAGTCACGAC−3’(SEQ ID NO:10)(表1、Df3)、例4に述べられたPCR法に用いられたプライマーDf1(SEQ ID NO:8)、表1に示されたオリゴヌクレオチドプライマーDf4、Df5がある。プライマーDf4 GGTGAATTAGCCATGCG−3’(SEQ ID NO:11)はDer p VII配列決定に用いられており、プライマーDf5 TCAATCTTGGATCCAATTTTTGGC−3’(SEQ ID NO:12)はDer f VII配列のヌクレオチド559−582に基づいている。
当業者は、日常的にすぎない実験法により、ここで述べられた特定の具体例と同等の方法及び物が使用できることを認識し又は確認できるであろう。そのような同等物はこの発明の対象とし、以下に続く請求の範囲に含まれるものとする。
Claims (17)
- SEQ ID NO:7のアミノ酸配列を含む蛋白質アレルゲンDer f VII、又はその成熟部分、又はその少なくとも1つのエピトープを含むペプチドをコードするヌクレオチド配列を含む単離された核酸。
- SEQ ID NO:6のヌクレオチド配列を含む、請求項1に記載の単離された核酸。
- SEQ ID NO:7のヌクレオチド配列のコード領域を含む、請求項2に記載の単離された核酸。
- 蛋白質アレルゲンが、SEQ ID NO:7のアミノ酸配列を含む、請求項1に記載の単離された核酸。
- 少なくとも1つのDermatophagoides由来のエピトープを含む蛋白質アレルゲン又はペプチドをコードする単離された核酸であって、該核酸が、高緊縮又は低緊縮条件下でSEQ ID NO:6に示したDer f VIIをコードする核酸の相補鎖とハイブリダイズする上記の単離された核酸。
- ペプチドが、少なくとも約10−20アミノ酸長である、請求項1に記載の単離された核酸。
- エピトープが、T細胞エピトープ又はB細胞エピトープである、請求項1に記載の単離された核酸。
- 請求項1〜7の何れか一つに記載の核酸を含む組換え発現ベクター。
- 請求項8に記載の組換え発現ベクターでトランスフェクトした宿主細胞。
- ハウスダストダニ蛋白質アレルゲン又はその少なくとも1つのエピトープを含むペプチドを製造する方法であって、請求項9に記載の宿主細胞を培地中で培養して、該蛋白質アレルゲン又はペプチドを発現させ、該蛋白質アレルゲン又はペプチドを該培養から単離することを含む当該方法。
- SEQ ID NO:7のアミノ酸配列を含む単離されたグループVIIの蛋白質アレルゲン、Der f VII、又はその成熟部分、又はその少なくとも1つのエピトープを含むペプチド。
- 蛋白質アレルゲン又はペプチドをコードする核酸の組換え発現により生成された、請求項11に記載の蛋白質アレルゲン又はペプチド。
- 化学合成により生成された、請求項11に記載の蛋白質アレルゲン又はペプチド。
- 少なくとも10−20アミノ酸長である、請求項11に記載の蛋白質アレルゲン又はペプチド。
- 個人におけるハウスダストダニに対する過敏症を治療し又は診断するための、請求項11〜14の何れか一つに記載の蛋白質アレルゲン又はペプチド。
- 患者におけるハウスダストダニアレルゲンに対する過敏症を検出する方法であって、患者から得られた血液試料を請求項11〜14の何れか一つに記載の蛋白質アレルゲン又はペプチドと、血液成分が該蛋白質又はペプチドと結合するのに適した条件下で合わせて、かかる結合がおきる程度を測定することを含む、上記の方法。
- 結合が起きる程度を、T細胞機能、T細胞増殖、B細胞機能、上記の蛋白質又はペプチドの血液中に存在する抗体への結合を評価することにより、又はこれらの組合せにより測定する、請求項16に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3114193A | 1993-03-12 | 1993-03-12 | |
US08/081,540 US6849427B1 (en) | 1993-03-12 | 1993-06-22 | Nucleic acids encoding a house dust mite allergen, Der p VII, and uses therefor |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51939394A Division JP3742426B2 (ja) | 1993-03-12 | 1994-03-11 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007010497A Division JP2007151555A (ja) | 1993-03-12 | 2007-01-19 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2004113249A true JP2004113249A (ja) | 2004-04-15 |
JP3934594B2 JP3934594B2 (ja) | 2007-06-20 |
Family
ID=26706883
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51939394A Expired - Lifetime JP3742426B2 (ja) | 1993-03-12 | 1994-03-11 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
JP2003383755A Expired - Lifetime JP3934594B2 (ja) | 1993-03-12 | 2003-11-13 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
JP2007010497A Pending JP2007151555A (ja) | 1993-03-12 | 2007-01-19 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51939394A Expired - Lifetime JP3742426B2 (ja) | 1993-03-12 | 1994-03-11 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007010497A Pending JP2007151555A (ja) | 1993-03-12 | 2007-01-19 | ハウスダストダニからのアレルゲン蛋白質、ペプチド及びそのための使用 |
Country Status (14)
Country | Link |
---|---|
US (5) | US6849427B1 (ja) |
EP (2) | EP0690914B1 (ja) |
JP (3) | JP3742426B2 (ja) |
AT (2) | ATE195343T1 (ja) |
AU (1) | AU690900B2 (ja) |
CA (1) | CA2158047C (ja) |
DE (2) | DE69434885T2 (ja) |
DK (2) | DK1018550T3 (ja) |
ES (2) | ES2277581T3 (ja) |
IL (2) | IL108921A (ja) |
NZ (1) | NZ262860A (ja) |
PT (2) | PT690914E (ja) |
WO (1) | WO1994020614A1 (ja) |
ZA (1) | ZA941713B (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018183105A (ja) * | 2017-04-27 | 2018-11-22 | 日光ケミカルズ株式会社 | 皮膚刺激評価方法 |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6849427B1 (en) * | 1993-03-12 | 2005-02-01 | Immulogic Pharmaceutical Corp. | Nucleic acids encoding a house dust mite allergen, Der p VII, and uses therefor |
WO1997024139A1 (en) * | 1995-12-29 | 1997-07-10 | University Of Arkansas | Peanut allergens and methods |
DE69838768T2 (de) | 1997-09-19 | 2008-10-30 | Metabolix, Inc., Cambridge | Biologische Systeme zur Herstellung von Polyhydroxyalkanoat-Polymeren die 4-Hy droxysäure enthalten |
EP1051494B1 (en) | 1998-01-31 | 2009-04-29 | The University of Arkansas | Methods and reagents for decreasing allergic reactions |
US7879977B2 (en) | 1998-01-31 | 2011-02-01 | University Of Arkansas | Methods and reagents for decreasing clinical reaction to allergy |
KR20020000218A (ko) * | 1999-02-25 | 2002-01-05 | 추후제출 | 네이키드 플라스미드 디엔에이를 이용한 집 먼지 진드기알레르겐용 백신 |
GB9924351D0 (en) | 1999-10-14 | 1999-12-15 | Brennan Frank | Immunomodulation methods and compositions |
US8246945B2 (en) | 2000-04-06 | 2012-08-21 | University Of Arkansas | Methods and reagents for decreasing clinical reaction to allergy |
DK1272213T3 (da) | 2000-04-06 | 2006-07-10 | Seer Pharmaceuticals Llc | Mikrobielt afgivelsessystem |
WO2002066602A2 (en) * | 2001-01-03 | 2002-08-29 | Heska Corporation | Detection of allergen-specific ige |
US7060687B2 (en) | 2001-02-07 | 2006-06-13 | Genmont Biotechnology Co. | Live vaccines for allergy treatment |
GB0120150D0 (en) * | 2001-08-17 | 2001-10-10 | Glaxosmithkline Biolog Sa | Novel compounds |
US20060251667A1 (en) * | 2002-08-29 | 2006-11-09 | Chua Kaw Y | Recombinant nucleic acid useful for inducing protective immune response against allergens |
JP4043387B2 (ja) * | 2003-03-18 | 2008-02-06 | 三菱重工業株式会社 | 空調用室内ユニット及びこれを備えた空気調和機 |
US20090111702A1 (en) * | 2004-04-06 | 2009-04-30 | Mount Sinai School Of Medicine Office Of Industrial Liason | Methods of determining allergen response using microarray immunoassay techniques |
US20060269576A1 (en) * | 2005-05-27 | 2006-11-30 | Curalogic, A/S | Non-injection immunotherapy |
AU2008279584A1 (en) * | 2007-04-27 | 2009-01-29 | Dow Global Technologies Inc. | Improved production and in vivo assembly of soluble recombinant icosahedral virus-like particles |
WO2009022154A2 (en) | 2007-08-15 | 2009-02-19 | Circassia Limited | Peptide with multiple epitopes |
US8859210B2 (en) * | 2008-10-17 | 2014-10-14 | Mead Johnson Nutrition Company | Method for identifying allergenic proteins and peptides |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4745051A (en) * | 1983-05-27 | 1988-05-17 | The Texas A&M University System | Method for producing a recombinant baculovirus expression vector |
US4879213A (en) * | 1986-12-05 | 1989-11-07 | Scripps Clinic And Research Foundation | Synthetic polypeptides and antibodies related to Epstein-Barr virus early antigen-diffuse |
PT87133B (pt) * | 1987-04-02 | 1992-07-31 | Amrad Corp Ltd | Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif |
US5077214A (en) * | 1989-07-07 | 1991-12-31 | The Texas A&M University System | Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells |
JP3139777B2 (ja) * | 1990-08-27 | 2001-03-05 | フマキラー株式会社 | 組換えダニアレルゲン |
US6849427B1 (en) * | 1993-03-12 | 2005-02-01 | Immulogic Pharmaceutical Corp. | Nucleic acids encoding a house dust mite allergen, Der p VII, and uses therefor |
-
1993
- 1993-06-22 US US08/081,540 patent/US6849427B1/en not_active Expired - Lifetime
-
1994
- 1994-03-10 IL IL108921A patent/IL108921A/en not_active IP Right Cessation
- 1994-03-11 DE DE69434885T patent/DE69434885T2/de not_active Expired - Lifetime
- 1994-03-11 CA CA2158047A patent/CA2158047C/en not_active Expired - Lifetime
- 1994-03-11 AT AT94910283T patent/ATE195343T1/de active
- 1994-03-11 US US08/553,336 patent/US6413738B1/en not_active Expired - Lifetime
- 1994-03-11 ES ES00102413T patent/ES2277581T3/es not_active Expired - Lifetime
- 1994-03-11 AT AT00102413T patent/ATE346149T1/de active
- 1994-03-11 NZ NZ262860A patent/NZ262860A/xx not_active IP Right Cessation
- 1994-03-11 ES ES94910283T patent/ES2150986T3/es not_active Expired - Lifetime
- 1994-03-11 AU AU62783/94A patent/AU690900B2/en not_active Expired
- 1994-03-11 DK DK00102413T patent/DK1018550T3/da active
- 1994-03-11 PT PT94910283T patent/PT690914E/pt unknown
- 1994-03-11 PT PT00102413T patent/PT1018550E/pt unknown
- 1994-03-11 DK DK94910283T patent/DK0690914T3/da active
- 1994-03-11 JP JP51939394A patent/JP3742426B2/ja not_active Expired - Lifetime
- 1994-03-11 ZA ZA941713A patent/ZA941713B/xx unknown
- 1994-03-11 DE DE69425490T patent/DE69425490T2/de not_active Expired - Lifetime
- 1994-03-11 WO PCT/AU1994/000117 patent/WO1994020614A1/en active IP Right Grant
- 1994-03-11 EP EP94910283A patent/EP0690914B1/en not_active Expired - Lifetime
- 1994-03-11 EP EP00102413A patent/EP1018550B1/en not_active Expired - Lifetime
-
1995
- 1995-06-05 US US08/462,778 patent/US6077517A/en not_active Expired - Lifetime
-
2001
- 2001-12-19 US US10/024,955 patent/US6905845B2/en not_active Expired - Fee Related
-
2002
- 2002-05-29 IL IL14990502A patent/IL149905A0/xx unknown
-
2003
- 2003-11-13 JP JP2003383755A patent/JP3934594B2/ja not_active Expired - Lifetime
-
2005
- 2005-03-17 US US11/084,057 patent/US8003382B2/en not_active Expired - Fee Related
-
2007
- 2007-01-19 JP JP2007010497A patent/JP2007151555A/ja active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018183105A (ja) * | 2017-04-27 | 2018-11-22 | 日光ケミカルズ株式会社 | 皮膚刺激評価方法 |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8003382B2 (en) | Nucleic acid molecules encoding a house dust mite allergen Der f VII, and antigenic peptides thereof | |
US7666441B2 (en) | Allergenic proteins and peptides from dog dander and uses therefor | |
US9447154B2 (en) | Nucleic acids encoding a house dust mite allergen, Der p III, and uses thereof | |
KR100263393B1 (ko) | 라이그라스 화분 알레르겐 | |
JP3220451B2 (ja) | ブタクサからのアレルゲン蛋白質およびそれらの使用 | |
JP2005503113A (ja) | ブタクサアレルゲン | |
WO1992016554A1 (en) | Protein allergens of the species cynodon dactylon | |
KR100733887B1 (ko) | DerpⅢ단백질알레르겐을암호화하는분리된핵산 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20050301 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20050509 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20050512 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050901 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20060720 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20061020 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20061025 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070119 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20070213 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20070315 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110330 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110330 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120330 Year of fee payment: 5 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130330 Year of fee payment: 6 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130330 Year of fee payment: 6 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140330 Year of fee payment: 7 |
|
EXPY | Cancellation because of completion of term |