JP2003517580A5 - - Google Patents

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Publication number
JP2003517580A5
JP2003517580A5 JP2000595151A JP2000595151A JP2003517580A5 JP 2003517580 A5 JP2003517580 A5 JP 2003517580A5 JP 2000595151 A JP2000595151 A JP 2000595151A JP 2000595151 A JP2000595151 A JP 2000595151A JP 2003517580 A5 JP2003517580 A5 JP 2003517580A5
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JP
Japan
Prior art keywords
gdf
activity
inhibitor
protein
isolated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000595151A
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English (en)
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JP2003517580A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2000/001552 external-priority patent/WO2000043781A2/en
Publication of JP2003517580A publication Critical patent/JP2003517580A/ja
Publication of JP2003517580A5 publication Critical patent/JP2003517580A5/ja
Pending legal-status Critical Current

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Description

【特許請求の範囲】
【請求項1】 (a)GDFタンパク質を生産する細胞を培養した培地を得る
こと;(b)GDF活性を阻害する能力に関して該培地を試験し、それによりGDFイ
ンヒビターを同定すること:
を含んでなる、GDFタンパク質のインヒビターの同定方法。
【請求項2】 (a)GDFタンパク質のフラグメントを調製すること;(
b)GDF活性を阻害する能力に関して該フラグメントを試験し、それによりGDFイ
ンヒビターを同定すること:
を含んでなる、GDFタンパク質のインヒビターの同定方法。
【請求項3】 ヒトGDF-8またはGDF-11をコードするインサートを含有す
る発現プラスミドで安定にトランスフェクションしたCHO細胞を単離した培地か
らイオン交換クロマトグラフィーにより単離することができ、100℃で10分まで
の間加熱した後に活性を保持し、還元後に活性を保持し、そして6M尿素での処理
後に活性を保持するGDF-8またはGDF-11インヒビター。
【請求項4】 ヒトGDF-8またはGDF-11をコードするインサートを含有す
る発現プラスミドで安定にトランスフェクションしたCHO細胞を単離した培地か
らイオン交換クロマトグラフィーにより単離することができ、100℃で10分まで
の間加熱した後に活性を保持し、還元後に活性を失い、そして6M尿素での処理後
に活性を保持するGDF-8またはGDF-11インヒビター。
【請求項5】 GDF活性を阻害するGDFタンパク質またはペプチド。
【請求項6】 GDFタンパク質のプロ-ドメインまたはプロ-ドメインの一
部を含んでなるGDFインヒビター。
【請求項7】 細胞にトランスフェクションするとGDF発現を阻害する、
配列番号:1、2、3及び4よりなる群から選択されるヌクレオチド配列を含んでな
る単離された核酸。
【請求項8】 細胞にトランスフェクションするとGDF発現を阻害する、
配列番号:5-24よりなる群から選択されるヌクレオチド配列を含んでなる単離さ
れた核酸。
【請求項9】 GDFタンパク質の変異体を含んでなるGDFインヒビター。
【請求項10】 細胞におけるGDF活性を阻害する、配列番号:25、29また
は30よりなる群から選択されるアミノ酸配列を含んでなる単離されたポリペプチ
ド。
【請求項11】 GDFインヒビターを発現する非ヒト動物。
JP2000595151A 1999-01-21 2000-01-21 増殖分化因子インヒビター及びそれらの用途 Pending JP2003517580A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11663999P 1999-01-21 1999-01-21
US60/116,639 1999-01-21
US13836399P 1999-06-10 1999-06-10
US60/138,363 1999-06-10
PCT/US2000/001552 WO2000043781A2 (en) 1999-01-21 2000-01-21 Growth differentiation factor inhibitors and uses therefor

Publications (2)

Publication Number Publication Date
JP2003517580A JP2003517580A (ja) 2003-05-27
JP2003517580A5 true JP2003517580A5 (ja) 2007-03-08

Family

ID=26814445

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000595151A Pending JP2003517580A (ja) 1999-01-21 2000-01-21 増殖分化因子インヒビター及びそれらの用途

Country Status (12)

Country Link
EP (1) EP1147413B1 (ja)
JP (1) JP2003517580A (ja)
CN (1) CN100567322C (ja)
AT (1) ATE322681T1 (ja)
AU (2) AU772694B2 (ja)
BR (1) BR0008188A (ja)
CA (1) CA2359242C (ja)
DE (1) DE60027135T2 (ja)
HK (1) HK1041521B (ja)
MX (1) MXPA01007366A (ja)
NZ (1) NZ513642A (ja)
WO (1) WO2000043781A2 (ja)

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