JP2003510254A - 核酸単離方法およびキット - Google Patents
核酸単離方法およびキットInfo
- Publication number
- JP2003510254A JP2003510254A JP2001525207A JP2001525207A JP2003510254A JP 2003510254 A JP2003510254 A JP 2003510254A JP 2001525207 A JP2001525207 A JP 2001525207A JP 2001525207 A JP2001525207 A JP 2001525207A JP 2003510254 A JP2003510254 A JP 2003510254A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- solid phase
- sample
- dna
- organic polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/902—Specified use of nanostructure
- Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
- Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/400,037 US6383783B1 (en) | 1999-09-21 | 1999-09-21 | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
| US09/400,037 | 1999-09-21 | ||
| PCT/US2000/002260 WO2001021632A1 (en) | 1999-09-21 | 2000-01-28 | Method for nucleic acid isolation and kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003510254A true JP2003510254A (ja) | 2003-03-18 |
| JP2003510254A5 JP2003510254A5 (https=) | 2007-03-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001525207A Pending JP2003510254A (ja) | 1999-09-21 | 2000-01-28 | 核酸単離方法およびキット |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US6383783B1 (https=) |
| EP (1) | EP1216255B1 (https=) |
| JP (1) | JP2003510254A (https=) |
| AU (1) | AU2745000A (https=) |
| DE (1) | DE60012549T2 (https=) |
| WO (1) | WO2001021632A1 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6383783B1 (en) * | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
| CA2399475C (en) * | 2000-02-04 | 2011-04-12 | Qiagen Gmbh | Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors |
| FR2808276B1 (fr) * | 2000-04-26 | 2004-04-02 | Renaud Nalin | Procede d'extraction indirecte de l'adn d'organismes non cultivables et adn susceptible d'etre obtenu par ledit procede |
| EP1334763B1 (en) * | 2000-10-24 | 2006-12-20 | Kaneka Corporation | Hydrophilized membrane and method of hydrophilization therefor |
| US7347976B2 (en) * | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US20040067495A1 (en) * | 2002-10-08 | 2004-04-08 | Ray Gustav Allen | Polynucleic acid and polyamino acid binding substrate |
| US7981600B2 (en) * | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
| US20050042660A1 (en) * | 2003-07-31 | 2005-02-24 | Hall Gerald Edward | Devices and methods for isolating RNA |
| US7031802B2 (en) * | 2003-08-13 | 2006-04-18 | Hewlett-Packard Development Company, L.P. | Semi-autonomous operation of a robotic device |
| JP2005095003A (ja) * | 2003-09-03 | 2005-04-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| DE10344820B4 (de) * | 2003-09-26 | 2009-04-16 | Sartorius Stedim Biotech Gmbh | Adsorptionsmembranen, Verfahren zur Herstellung derselben und Verwendung der Adsorptionsmembranen in Vorrichtungen |
| DE10344819B4 (de) * | 2003-09-26 | 2017-06-29 | Sartorius Stedim Biotech Gmbh | Adsorptionsmembranen, Verfahren zur Herstellung derselben und Vorrichtungen, welche die Adsorptionsmembranen umfassen |
| US20080161549A1 (en) * | 2003-10-24 | 2008-07-03 | Rosetta Inpharmatics Llc | Method of purifying nucleic acid molecules using proteinase k |
| US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7939249B2 (en) * | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| US7727710B2 (en) * | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
| US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| US7531308B2 (en) * | 2004-04-23 | 2009-05-12 | Sigma-Aldrich Co. | Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography |
| US20060099605A1 (en) * | 2004-11-11 | 2006-05-11 | Hall Gerald E Jr | Devices and methods for isolating RNA |
| US20060270843A1 (en) * | 2005-05-26 | 2006-11-30 | Hall Gerald E Jr | Methods for isolation of nucleic acids |
| US20060269929A1 (en) * | 2005-05-26 | 2006-11-30 | Hall Gerald E Jr | Methods and kits for DNA purification on polymeric membranes at low ionic strength |
| WO2007094945A2 (en) * | 2006-02-09 | 2007-08-23 | Waters Investments Limited | Mobil bead configuration immunoaffinity column and methods of use |
| KR100785010B1 (ko) * | 2006-04-06 | 2007-12-11 | 삼성전자주식회사 | 수소 결합을 이용하여 고체 지지체의 친수성 표면 상에서핵산 정제 방법 및 장치 |
| KR100785016B1 (ko) * | 2006-05-22 | 2007-12-12 | 삼성전자주식회사 | 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치 |
| US20100129878A1 (en) * | 2007-04-25 | 2010-05-27 | Parthasarathy Ranjani V | Methods for nucleic acid amplification |
| WO2016130553A1 (en) * | 2015-02-13 | 2016-08-18 | Siemens Healthcare Diagnostics Inc. | Methods and apparatus providing reduced carryover during pipetting operations |
| CN111148847B (zh) * | 2017-10-11 | 2024-05-07 | 深圳华大智造科技有限公司 | 用于改善核酸装载和稳定性的方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08501321A (ja) * | 1993-07-01 | 1996-02-13 | キアゲン・ゲーエムベーハー | クロマトグラフィーによる核酸混合物の精製分離法 |
| JPH10155481A (ja) * | 1996-06-18 | 1998-06-16 | Rikagaku Kenkyusho | Ｄｎａの回収方法 |
| WO1999010527A2 (de) * | 1997-08-21 | 1999-03-04 | Skw Trostberg Aktiengesellschaft | Verfahren zur isolierung von anionischen organischen substanzen aus wässrigen systemen mit kationischen polymernanopartikeln |
| WO1999022021A1 (de) * | 1997-10-23 | 1999-05-06 | Qiagen Gesellschaft Mit Beschränkter Haftung | Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen |
| WO1999029703A2 (en) * | 1997-12-06 | 1999-06-17 | Dna Research Instruments Limited | Isolation of nucleic acids |
| WO2000049031A2 (de) * | 1999-02-19 | 2000-08-24 | Bayer Aktiengesellschaft | Verfahren zur isolierung von nucleinsäuren |
Family Cites Families (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4153661A (en) | 1977-08-25 | 1979-05-08 | Minnesota Mining And Manufacturing Company | Method of making polytetrafluoroethylene composite sheet |
| US4373519A (en) | 1981-06-26 | 1983-02-15 | Minnesota Mining And Manufacturing Company | Composite wound dressing |
| US4565663A (en) | 1981-06-26 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Method for making water-swellable composite sheet |
| US4460642A (en) | 1981-06-26 | 1984-07-17 | Minnesota Mining And Manufacturing Company | Water-swellable composite sheet of microfibers of PTFE and hydrophilic absorptive particles |
| US4483920A (en) | 1982-05-17 | 1984-11-20 | Hahnemann University | Immobilization of message RNA directly from cells onto filter material |
| US4539256A (en) | 1982-09-09 | 1985-09-03 | Minnesota Mining And Manufacturing Co. | Microporous sheet material, method of making and articles made therewith |
| US4726989A (en) | 1986-12-11 | 1988-02-23 | Minnesota Mining And Manufacturing | Microporous materials incorporating a nucleating agent and methods for making same |
| JPH0611747B2 (ja) | 1987-11-19 | 1994-02-16 | 大正製薬株式会社 | スルホンアニリド化合物 |
| US4882147A (en) | 1987-12-16 | 1989-11-21 | The Research Foundation Of State University Of New York | Novel polynucleotide analogs, methods for inhibiting nucleic acid polymerases and methods for inducing synthesis of interferon |
| US4971736A (en) | 1987-12-28 | 1990-11-20 | Minnesota Mining And Manufacturing Company | Method of preparing composite chromatographic article |
| US4923978A (en) | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
| US4810381A (en) | 1987-12-28 | 1989-03-07 | Minnesota Mining And Manufacturing Company | Composite chromatographic article |
| US4906378A (en) | 1987-12-28 | 1990-03-06 | Minnesota Mining And Manufacturing Company | Composite chromatographic article |
| DE3921498A1 (de) | 1988-09-28 | 1990-03-29 | Bayer Ag | Polymer-gebundene-farbstoffe, verfahren zu deren herstellung und verwendung |
| US4957943A (en) | 1988-10-14 | 1990-09-18 | Minnesota Mining And Manufacturing Company | Particle-filled microporous materials |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| JPH02268682A (ja) | 1989-04-07 | 1990-11-02 | Hitachi Ltd | Ｄｎａ試料調製方法 |
| JPH0824583B2 (ja) | 1989-05-09 | 1996-03-13 | 株式会社ニッショー | Ｄｎａの抽出方法 |
| US5231015A (en) | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
| US5207915A (en) | 1990-02-23 | 1993-05-04 | Minnesota Mining And Manufacturing Company | Separation method using controlled pore composite polytetrafluoroethylene article |
| US5147539A (en) | 1990-02-23 | 1992-09-15 | Minnesota Mining And Manufacturing Company | Controlled pore composite polytetrafluoroethylene article |
| US5071610A (en) | 1990-02-23 | 1991-12-10 | Minnesota Mining And Manufacturing Company | Method of making a controlled pore composite polytetrafluoroethylene |
| US5187083A (en) | 1990-11-13 | 1993-02-16 | Specialty Laboratories, Inc. | Rapid purification of DNA |
| CA2059398C (en) | 1991-02-07 | 1999-05-25 | Craig G. Markell | Solid phase extraction medium |
| WO1992018514A1 (en) | 1991-04-12 | 1992-10-29 | Minnesota Mining And Manufacturing Company | Purification of nucleic acids using metal oxide supports |
| CA2067711C (en) | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
| US5238621A (en) | 1991-06-28 | 1993-08-24 | Minnesota Mining And Manufacturing Company | Method of controlling porosity in a composite article |
| FR2679255B1 (fr) | 1991-07-17 | 1993-10-22 | Bio Merieux | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
| US5438128A (en) | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| CA2096193A1 (en) | 1992-06-01 | 1993-12-02 | Daniel L. Woodard | Chemically synthesized silanes that bind nucleic acids |
| EP0647232B1 (en) | 1992-06-23 | 1999-04-28 | Minnesota Mining And Manufacturing Company | Deproteinization with azlactone-functional supports |
| CA2102264C (en) | 1992-11-13 | 2000-08-01 | Daniel Lee Woodard | Boron silicates, aluminum silicates, phosphosilicates and purification of dna |
| AT398973B (de) | 1992-11-18 | 1995-02-27 | Bonn Guenther Dr | Verfahren zur trennung von nukleinsäuren |
| US5637687A (en) | 1993-08-31 | 1997-06-10 | Wiggins; James C. | Methods and compositions for isolating nucleic acids |
| US5438127A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane |
| US5438129A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant |
| KR100230909B1 (ko) | 1993-11-29 | 1999-12-01 | 다니엘 엘. 캐시앙 | 광범위의 유기체로부터 핵산 추출 방법 |
| US5610287A (en) | 1993-12-06 | 1997-03-11 | Molecular Tool, Inc. | Method for immobilizing nucleic acid molecules |
| US5625053A (en) | 1994-08-26 | 1997-04-29 | Board Of Regents For Northern Illinois Univ. | Method of isolating purified plasmid DNA using a nonionic detergent, solution |
| US5472600A (en) | 1995-02-01 | 1995-12-05 | Minnesota Mining And Manufacturing Company | Gradient density filter |
| JPH09302A (ja) | 1995-06-16 | 1997-01-07 | Rieko Moto | 履 物 |
| DE19530132C2 (de) | 1995-08-16 | 1998-07-16 | Max Planck Gesellschaft | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
| US5804684A (en) | 1995-08-24 | 1998-09-08 | The Theobald Smith Research Institute, Inc. | Method for isolating nucleic acids |
| JPH09302034A (ja) | 1996-05-14 | 1997-11-25 | Makoto Komiyama | 核酸誘導体および尿素の吸着除去可能な高分子材料 |
| US6093559A (en) * | 1996-10-24 | 2000-07-25 | Corning Incorporated | Producing low binding hydrophobic surfaces by treating with a low HLB number non-ionic surfactant |
| US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| EP0897978A3 (en) | 1997-08-22 | 2001-10-17 | Becton, Dickinson and Company | Zirconium oxide and related compounds for purification of nucleic acids |
| FI974124A0 (fi) | 1997-11-04 | 1997-11-04 | Satu Aokerman | Foerfarande foer separering av proteinfria aemnen fraon proteinhaltiga aemnen foer daerpao foeljande behandling |
| US6475722B1 (en) | 1997-12-03 | 2002-11-05 | Curagen Corporation | Surface treatments for DNA processing devices |
| US6383783B1 (en) * | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
-
1999
- 1999-09-21 US US09/400,037 patent/US6383783B1/en not_active Expired - Fee Related
-
2000
- 2000-01-28 AU AU27450/00A patent/AU2745000A/en not_active Abandoned
- 2000-01-28 DE DE60012549T patent/DE60012549T2/de not_active Expired - Lifetime
- 2000-01-28 EP EP00905828A patent/EP1216255B1/en not_active Expired - Lifetime
- 2000-01-28 JP JP2001525207A patent/JP2003510254A/ja active Pending
- 2000-01-28 WO PCT/US2000/002260 patent/WO2001021632A1/en not_active Ceased
-
2002
- 2002-05-02 US US10/137,561 patent/US6790642B2/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08501321A (ja) * | 1993-07-01 | 1996-02-13 | キアゲン・ゲーエムベーハー | クロマトグラフィーによる核酸混合物の精製分離法 |
| JPH10155481A (ja) * | 1996-06-18 | 1998-06-16 | Rikagaku Kenkyusho | Ｄｎａの回収方法 |
| WO1999010527A2 (de) * | 1997-08-21 | 1999-03-04 | Skw Trostberg Aktiengesellschaft | Verfahren zur isolierung von anionischen organischen substanzen aus wässrigen systemen mit kationischen polymernanopartikeln |
| WO1999022021A1 (de) * | 1997-10-23 | 1999-05-06 | Qiagen Gesellschaft Mit Beschränkter Haftung | Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen |
| WO1999029703A2 (en) * | 1997-12-06 | 1999-06-17 | Dna Research Instruments Limited | Isolation of nucleic acids |
| WO2000049031A2 (de) * | 1999-02-19 | 2000-08-24 | Bayer Aktiengesellschaft | Verfahren zur isolierung von nucleinsäuren |
Also Published As
| Publication number | Publication date |
|---|---|
| US6383783B1 (en) | 2002-05-07 |
| WO2001021632A1 (en) | 2001-03-29 |
| US6790642B2 (en) | 2004-09-14 |
| AU2745000A (en) | 2001-04-24 |
| EP1216255A1 (en) | 2002-06-26 |
| US20030018177A1 (en) | 2003-01-23 |
| DE60012549D1 (de) | 2004-09-02 |
| EP1216255B1 (en) | 2004-07-28 |
| DE60012549T2 (de) | 2005-09-01 |
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