JP2002518016A - Ｎｅｋ関連及びｂｕｂ１関連プロテインキナーゼ - Google Patents

Info

Publication number

JP2002518016A

JP2002518016A JP2000554860A JP2000554860A JP2002518016A JP 2002518016 A JP2002518016 A JP 2002518016A JP 2000554860 A JP2000554860 A JP 2000554860A JP 2000554860 A JP2000554860 A JP 2000554860A JP 2002518016 A JP2002518016 A JP 2002518016A

Authority

JP

Japan

Prior art keywords

seq

amino acid

kinase

acid sequence

kinase polypeptide

Prior art date

1998-06-16

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Withdrawn

Links

• Espacenet
• Global Dossier
• Discuss

• 101000896657 Homo sapiens Mitotic checkpoint serine/threonine-protein kinase BUB1 Proteins 0.000 title claims abstract description 206
• 102100021691 Mitotic checkpoint serine/threonine-protein kinase BUB1 Human genes 0.000 title abstract description 159
• 102000001253 Protein Kinase Human genes 0.000 title description 40
• 108060006633 protein kinase Proteins 0.000 title description 40
• 108091000080 Phosphotransferase Proteins 0.000 claims abstract description 319
• 102000020233 phosphotransferase Human genes 0.000 claims abstract description 310
• 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 238
• 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 217
• 229920001184 polypeptide Polymers 0.000 claims abstract description 210
• 238000000034 method Methods 0.000 claims abstract description 196
• 108090000623 proteins and genes Proteins 0.000 claims abstract description 160
• 102000004169 proteins and genes Human genes 0.000 claims abstract description 102
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 69
• 201000010099 disease Diseases 0.000 claims abstract description 49
• 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 46
• 238000011282 treatment Methods 0.000 claims abstract description 26
• 210000004027 cell Anatomy 0.000 claims description 255
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 171
• 150000007523 nucleic acids Chemical class 0.000 claims description 148
• 102000039446 nucleic acids Human genes 0.000 claims description 115
• 108020004707 nucleic acids Proteins 0.000 claims description 115
• 125000003729 nucleotide group Chemical group 0.000 claims description 85
• 239000002773 nucleotide Substances 0.000 claims description 84
• 239000000523 sample Substances 0.000 claims description 78
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 68
• 230000000694 effects Effects 0.000 claims description 64
• 230000014509 gene expression Effects 0.000 claims description 64
• 101000601467 Homo sapiens Serine/threonine-protein kinase Nek5 Proteins 0.000 claims description 63
• 210000004899 c-terminal region Anatomy 0.000 claims description 60
• 101000588540 Homo sapiens Serine/threonine-protein kinase Nek6 Proteins 0.000 claims description 59
• 230000003197 catalytic effect Effects 0.000 claims description 57
• 206010028980 Neoplasm Diseases 0.000 claims description 45
• 102100037702 Serine/threonine-protein kinase Nek5 Human genes 0.000 claims description 43
• 239000012634 fragment Substances 0.000 claims description 43
• 230000027455 binding Effects 0.000 claims description 40
• 239000013598 vector Substances 0.000 claims description 39
• 102100031401 Serine/threonine-protein kinase Nek6 Human genes 0.000 claims description 38
• 201000011510 cancer Diseases 0.000 claims description 38
• 239000000126 substance Substances 0.000 claims description 37
• 101150076596 bub-1 gene Proteins 0.000 claims description 35
• 238000009396 hybridization Methods 0.000 claims description 33
• 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 32
• 239000002853 nucleic acid probe Substances 0.000 claims description 32
• 239000000074 antisense oligonucleotide Substances 0.000 claims description 26
• 238000012230 antisense oligonucleotides Methods 0.000 claims description 26
• 238000003556 assay Methods 0.000 claims description 24
• 230000000295 complement effect Effects 0.000 claims description 24
• 108091034117 Oligonucleotide Proteins 0.000 claims description 23
• 238000000338 in vitro Methods 0.000 claims description 23
• 238000012360 testing method Methods 0.000 claims description 23
• 208000035475 disorder Diseases 0.000 claims description 20
• 230000035897 transcription Effects 0.000 claims description 20
• 238000013518 transcription Methods 0.000 claims description 20
• 125000000539 amino acid group Chemical group 0.000 claims description 18
• 241000124008 Mammalia Species 0.000 claims description 13
• 210000004408 hybridoma Anatomy 0.000 claims description 13
• 230000003993 interaction Effects 0.000 claims description 10
• 230000009870 specific binding Effects 0.000 claims description 10
• 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 7
• 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 7
• 208000026278 immune system disease Diseases 0.000 claims description 7
• 208000015122 neurodegenerative disease Diseases 0.000 claims description 7
• 208000008839 Kidney Neoplasms Diseases 0.000 claims description 6
• 206010038389 Renal cancer Diseases 0.000 claims description 6
• 201000010982 kidney cancer Diseases 0.000 claims description 6
• 208000032839 leukemia Diseases 0.000 claims description 6
• 206010006187 Breast cancer Diseases 0.000 claims description 5
• 208000026310 Breast neoplasm Diseases 0.000 claims description 5
• 206010009944 Colon cancer Diseases 0.000 claims description 5
• 206010025323 Lymphomas Diseases 0.000 claims description 5
• 206010033128 Ovarian cancer Diseases 0.000 claims description 5
• 206010061535 Ovarian neoplasm Diseases 0.000 claims description 5
• 206010060862 Prostate cancer Diseases 0.000 claims description 5
• 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 5
• 201000007455 central nervous system cancer Diseases 0.000 claims description 5
• 208000029742 colonic neoplasm Diseases 0.000 claims description 5
• 201000001441 melanoma Diseases 0.000 claims description 5
• 206010008342 Cervix carcinoma Diseases 0.000 claims description 4
• 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 4
• 208000006105 Uterine Cervical Neoplasms Diseases 0.000 claims description 4
• 201000010881 cervical cancer Diseases 0.000 claims description 4
• 201000005202 lung cancer Diseases 0.000 claims description 4
• 208000020816 lung neoplasm Diseases 0.000 claims description 4
• 230000002401 inhibitory effect Effects 0.000 claims description 3
• 239000003054 catalyst Substances 0.000 claims 2
• 102000001783 NIMA-Related Kinases Human genes 0.000 abstract description 37
• 108010029888 NIMA-Related Kinases Proteins 0.000 abstract description 37
• 101001059454 Homo sapiens Serine/threonine-protein kinase MARK2 Proteins 0.000 abstract description 27
• 102100028904 Serine/threonine-protein kinase MARK2 Human genes 0.000 abstract description 27
• 102000046074 human BUB1 Human genes 0.000 abstract description 19
• 238000010367 cloning Methods 0.000 abstract description 10
• 101150073911 STK gene Proteins 0.000 abstract description 2
• 238000003745 diagnosis Methods 0.000 abstract description 2
• 229940024606 amino acid Drugs 0.000 description 150
• 235000001014 amino acid Nutrition 0.000 description 149
• 150000001413 amino acids Chemical class 0.000 description 148
• 235000018102 proteins Nutrition 0.000 description 96
• 108020004414 DNA Proteins 0.000 description 66
• 150000001875 compounds Chemical class 0.000 description 63
• 230000006870 function Effects 0.000 description 44
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 34
• 210000001519 tissue Anatomy 0.000 description 33
• 239000003153 chemical reaction reagent Substances 0.000 description 31
• 230000001105 regulatory effect Effects 0.000 description 31
• 239000002299 complementary DNA Substances 0.000 description 30
• 238000006366 phosphorylation reaction Methods 0.000 description 30
• 230000026731 phosphorylation Effects 0.000 description 29
• 239000000243 solution Substances 0.000 description 29
• 239000000203 mixture Substances 0.000 description 28
• 101000601460 Homo sapiens Serine/threonine-protein kinase Nek4 Proteins 0.000 description 26
• 102100037705 Serine/threonine-protein kinase Nek4 Human genes 0.000 description 26
• 241001465754 Metazoa Species 0.000 description 23
• 239000000463 material Substances 0.000 description 23
• 230000002159 abnormal effect Effects 0.000 description 22
• 239000000284 extract Substances 0.000 description 22
• 239000000758 substrate Substances 0.000 description 22
• -1 tyrosine amino acids Chemical class 0.000 description 21
• 230000004913 activation Effects 0.000 description 20
• 230000022131 cell cycle Effects 0.000 description 20
• 230000011278 mitosis Effects 0.000 description 19
• 239000013612 plasmid Substances 0.000 description 18
• 238000002347 injection Methods 0.000 description 17
• 239000007924 injection Substances 0.000 description 17
• 210000000349 chromosome Anatomy 0.000 description 16
• 239000003814 drug Substances 0.000 description 16
• 238000004458 analytical method Methods 0.000 description 15
• 238000001514 detection method Methods 0.000 description 14
• 238000012546 transfer Methods 0.000 description 14
• 238000003752 polymerase chain reaction Methods 0.000 description 13
• 108020004705 Codon Proteins 0.000 description 12
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
• 101000601441 Homo sapiens Serine/threonine-protein kinase Nek2 Proteins 0.000 description 12
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 12
• IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 12
• 239000000499 gel Substances 0.000 description 12
• 230000036961 partial effect Effects 0.000 description 12
• 239000000047 product Substances 0.000 description 12
• 101000886209 Homo sapiens Cyclin-G-associated kinase Proteins 0.000 description 11
• 101000601456 Homo sapiens Serine/threonine-protein kinase Nek3 Proteins 0.000 description 11
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 11
• 239000002585 base Substances 0.000 description 11
• 238000006243 chemical reaction Methods 0.000 description 11
• 229940079593 drug Drugs 0.000 description 11
• 238000009472 formulation Methods 0.000 description 11
• 238000001727 in vivo Methods 0.000 description 11
• 230000009261 transgenic effect Effects 0.000 description 11
• 108020000948 Antisense Oligonucleotides Proteins 0.000 description 10
• 102000004190 Enzymes Human genes 0.000 description 10
• 108090000790 Enzymes Proteins 0.000 description 10
• 108700039691 Genetic Promoter Regions Proteins 0.000 description 10
• 241000238631 Hexapoda Species 0.000 description 10
• 101100516358 Homo sapiens NEK5 gene Proteins 0.000 description 10
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 10
• 239000000872 buffer Substances 0.000 description 10
• 229940088598 enzyme Drugs 0.000 description 10
• 230000001965 increasing effect Effects 0.000 description 10
• 238000000021 kinase assay Methods 0.000 description 10
• 238000005259 measurement Methods 0.000 description 10
• 108020004999 messenger RNA Proteins 0.000 description 10
• 238000002360 preparation method Methods 0.000 description 10
• 230000001225 therapeutic effect Effects 0.000 description 10
• 210000004881 tumor cell Anatomy 0.000 description 10
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
• 101150012716 CDK1 gene Proteins 0.000 description 9
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 9
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 9
• 239000004472 Lysine Substances 0.000 description 9
• 238000012217 deletion Methods 0.000 description 9
• 230000037430 deletion Effects 0.000 description 9
• 210000003527 eukaryotic cell Anatomy 0.000 description 9
• 238000002372 labelling Methods 0.000 description 9
• 238000004519 manufacturing process Methods 0.000 description 9
• 239000002609 medium Substances 0.000 description 9
• 239000012528 membrane Substances 0.000 description 9
• 229930182817 methionine Natural products 0.000 description 9
• 239000008194 pharmaceutical composition Substances 0.000 description 9
• 239000000546 pharmaceutical excipient Substances 0.000 description 9
• 230000008488 polyadenylation Effects 0.000 description 9
• 238000011160 research Methods 0.000 description 9
• 239000000725 suspension Substances 0.000 description 9
• 241000351920 Aspergillus nidulans Species 0.000 description 8
• IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
• 101001123846 Homo sapiens Serine/threonine-protein kinase Nek1 Proteins 0.000 description 8
• 108091054455 MAP kinase family Proteins 0.000 description 8
• 102000043136 MAP kinase family Human genes 0.000 description 8
• 241000283973 Oryctolagus cuniculus Species 0.000 description 8
• 108010029485 Protein Isoforms Proteins 0.000 description 8
• 102000001708 Protein Isoforms Human genes 0.000 description 8
• 102100037703 Serine/threonine-protein kinase Nek2 Human genes 0.000 description 8
• 210000002230 centromere Anatomy 0.000 description 8
• 230000008859 change Effects 0.000 description 8
• 239000013613 expression plasmid Substances 0.000 description 8
• JYGFTBXVXVMTGB-UHFFFAOYSA-N indolin-2-one Chemical class C1=CC=C2NC(=O)CC2=C1 JYGFTBXVXVMTGB-UHFFFAOYSA-N 0.000 description 8
• 230000000394 mitotic effect Effects 0.000 description 8
• YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 8
• 102000005962 receptors Human genes 0.000 description 8
• 239000007787 solid Substances 0.000 description 8
• 230000005030 transcription termination Effects 0.000 description 8
• 238000013519 translation Methods 0.000 description 8
• 238000001262 western blot Methods 0.000 description 8
• 201000002829 CREST Syndrome Diseases 0.000 description 7
• 108091026890 Coding region Proteins 0.000 description 7
• 102100039683 Cyclin-G-associated kinase Human genes 0.000 description 7
• 241000588724 Escherichia coli Species 0.000 description 7
• 241000282412 Homo Species 0.000 description 7
• 108700026244 Open Reading Frames Proteins 0.000 description 7
• 108010076504 Protein Sorting Signals Proteins 0.000 description 7
• 239000000427 antigen Substances 0.000 description 7
• 108091007433 antigens Proteins 0.000 description 7
• 102000036639 antigens Human genes 0.000 description 7
• 239000006184 cosolvent Substances 0.000 description 7
• 238000001415 gene therapy Methods 0.000 description 7
• 230000002068 genetic effect Effects 0.000 description 7
• 102000054213 human NEK3 Human genes 0.000 description 7
• 230000000977 initiatory effect Effects 0.000 description 7
• 238000002955 isolation Methods 0.000 description 7
• 210000004185 liver Anatomy 0.000 description 7
• 239000002953 phosphate buffered saline Substances 0.000 description 7
• 108020003175 receptors Proteins 0.000 description 7
• 150000003839 salts Chemical class 0.000 description 7
• 230000007704 transition Effects 0.000 description 7
• 241000701447 unidentified baculovirus Species 0.000 description 7
• CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
• 241000894006 Bacteria Species 0.000 description 6
• DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 6
• 108091007914 CDKs Proteins 0.000 description 6
• 241000283707 Capra Species 0.000 description 6
• 102100030011 Endoribonuclease Human genes 0.000 description 6
• 101710199605 Endoribonuclease Proteins 0.000 description 6
• 108010010803 Gelatin Proteins 0.000 description 6
• 101100516340 Mus musculus Nek1 gene Proteins 0.000 description 6
• 238000000636 Northern blotting Methods 0.000 description 6
• 108020004511 Recombinant DNA Proteins 0.000 description 6
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 6
• 101710113029 Serine/threonine-protein kinase Proteins 0.000 description 6
• 102100028751 Serine/threonine-protein kinase Nek1 Human genes 0.000 description 6
• 238000007792 addition Methods 0.000 description 6
• 230000003321 amplification Effects 0.000 description 6
• 230000006907 apoptotic process Effects 0.000 description 6
• IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 6
• 238000004113 cell culture Methods 0.000 description 6
• 239000003795 chemical substances by application Substances 0.000 description 6
• 230000001419 dependent effect Effects 0.000 description 6
• 238000005516 engineering process Methods 0.000 description 6
• 239000008273 gelatin Substances 0.000 description 6
• 229920000159 gelatin Polymers 0.000 description 6
• 235000019322 gelatine Nutrition 0.000 description 6
• 235000011852 gelatine desserts Nutrition 0.000 description 6
• 230000002209 hydrophobic effect Effects 0.000 description 6
• 230000003053 immunization Effects 0.000 description 6
• 230000005764 inhibitory process Effects 0.000 description 6
• 230000003834 intracellular effect Effects 0.000 description 6
• 239000002502 liposome Substances 0.000 description 6
• 210000004962 mammalian cell Anatomy 0.000 description 6
• 230000031864 metaphase Effects 0.000 description 6
• 238000003199 nucleic acid amplification method Methods 0.000 description 6
• 210000004940 nucleus Anatomy 0.000 description 6
• 229920002401 polyacrylamide Polymers 0.000 description 6
• 230000008569 process Effects 0.000 description 6
• 238000003786 synthesis reaction Methods 0.000 description 6
• 229940104230 thymidine Drugs 0.000 description 6
• 241000701161 unidentified adenovirus Species 0.000 description 6
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 5
• 108091035707 Consensus sequence Proteins 0.000 description 5
• 102000053602 DNA Human genes 0.000 description 5
• 239000007995 HEPES buffer Substances 0.000 description 5
• 102000006947 Histones Human genes 0.000 description 5
• 108010033040 Histones Proteins 0.000 description 5
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 5
• 108091022875 Microtubule Proteins 0.000 description 5
• 102000029749 Microtubule Human genes 0.000 description 5
• 101100186871 Mus musculus Nek6 gene Proteins 0.000 description 5
• 102000004022 Protein-Tyrosine Kinases Human genes 0.000 description 5
• 108090000412 Protein-Tyrosine Kinases Proteins 0.000 description 5
• 241000700159 Rattus Species 0.000 description 5
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 5
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 5
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 5
• 239000004473 Threonine Substances 0.000 description 5
• 241000700605 Viruses Species 0.000 description 5
• 239000002253 acid Substances 0.000 description 5
• 239000004480 active ingredient Substances 0.000 description 5
• 230000015572 biosynthetic process Effects 0.000 description 5
• 210000004369 blood Anatomy 0.000 description 5
• 239000008280 blood Substances 0.000 description 5
• 239000002775 capsule Substances 0.000 description 5
• 239000000969 carrier Substances 0.000 description 5
• 230000001413 cellular effect Effects 0.000 description 5
• 239000002552 dosage form Substances 0.000 description 5
• 230000001605 fetal effect Effects 0.000 description 5
• 108010002838 hematopoietic progenitor kinase 1 Proteins 0.000 description 5
• 238000003018 immunoassay Methods 0.000 description 5
• 210000003734 kidney Anatomy 0.000 description 5
• 230000021121 meiosis Effects 0.000 description 5
• 210000004688 microtubule Anatomy 0.000 description 5
• 238000002156 mixing Methods 0.000 description 5
• 230000004048 modification Effects 0.000 description 5
• 238000012986 modification Methods 0.000 description 5
• 230000035772 mutation Effects 0.000 description 5
• 230000002018 overexpression Effects 0.000 description 5
• 239000002245 particle Substances 0.000 description 5
• 229920003023 plastic Polymers 0.000 description 5
• 239000004033 plastic Substances 0.000 description 5
• 229920001223 polyethylene glycol Polymers 0.000 description 5
• 239000001267 polyvinylpyrrolidone Substances 0.000 description 5
• 229920000036 polyvinylpyrrolidone Polymers 0.000 description 5
• 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 5
• 210000001236 prokaryotic cell Anatomy 0.000 description 5
• 238000000746 purification Methods 0.000 description 5
• 108091008146 restriction endonucleases Proteins 0.000 description 5
• 238000012216 screening Methods 0.000 description 5
• 238000012163 sequencing technique Methods 0.000 description 5
• 230000019491 signal transduction Effects 0.000 description 5
• 230000011664 signaling Effects 0.000 description 5
• 238000006467 substitution reaction Methods 0.000 description 5
• 235000000346 sugar Nutrition 0.000 description 5
• 210000001541 thymus gland Anatomy 0.000 description 5
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 5
• 239000013603 viral vector Substances 0.000 description 5
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
• 108010039627 Aprotinin Proteins 0.000 description 4
• 239000004475 Arginine Substances 0.000 description 4
• 241000701822 Bovine papillomavirus Species 0.000 description 4
• 102000052052 Casein Kinase II Human genes 0.000 description 4
• 108010010919 Casein Kinase II Proteins 0.000 description 4
• 235000019750 Crude protein Nutrition 0.000 description 4
• 102000016736 Cyclin Human genes 0.000 description 4
• 108050006400 Cyclin Proteins 0.000 description 4
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
• 241000206602 Eukaryota Species 0.000 description 4
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
• 101000633314 Homo sapiens Nicotinamide riboside kinase 2 Proteins 0.000 description 4
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 4
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 4
• 101100494310 Mus musculus Bub1 gene Proteins 0.000 description 4
• 241000699670 Mus sp. Species 0.000 description 4
• 101710141454 Nucleoprotein Proteins 0.000 description 4
• 108090000315 Protein Kinase C Proteins 0.000 description 4
• 102000003923 Protein Kinase C Human genes 0.000 description 4
• 230000018199 S phase Effects 0.000 description 4
• 102100037706 Serine/threonine-protein kinase Nek3 Human genes 0.000 description 4
• 229920002472 Starch Polymers 0.000 description 4
• 241000187747 Streptomyces Species 0.000 description 4
• 229920004890 Triton X-100 Polymers 0.000 description 4
• 229960000583 acetic acid Drugs 0.000 description 4
• 230000000692 anti-sense effect Effects 0.000 description 4
• SEKZNWAQALMJNH-YZUCACDQSA-N aphidicolin Natural products C[C@]1(CO)CC[C@]23C[C@H]1C[C@@H]2CC[C@H]4[C@](C)(CO)[C@H](O)CC[C@]34C SEKZNWAQALMJNH-YZUCACDQSA-N 0.000 description 4
• NOFOAYPPHIUXJR-APNQCZIXSA-N aphidicolin Chemical compound C1[C@@]23[C@@]4(C)CC[C@@H](O)[C@@](C)(CO)[C@@H]4CC[C@H]3C[C@H]1[C@](CO)(O)CC2 NOFOAYPPHIUXJR-APNQCZIXSA-N 0.000 description 4
• 229960004405 aprotinin Drugs 0.000 description 4
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 4
• CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
• 230000035578 autophosphorylation Effects 0.000 description 4
• 238000000376 autoradiography Methods 0.000 description 4
• 230000008901 benefit Effects 0.000 description 4
• 239000013060 biological fluid Substances 0.000 description 4
• 230000024245 cell differentiation Effects 0.000 description 4
• 230000032823 cell division Effects 0.000 description 4
• 230000010261 cell growth Effects 0.000 description 4
• 239000013592 cell lysate Substances 0.000 description 4
• 229920002678 cellulose Polymers 0.000 description 4
• 239000001913 cellulose Substances 0.000 description 4
• 235000010980 cellulose Nutrition 0.000 description 4
• 210000001072 colon Anatomy 0.000 description 4
• 238000010276 construction Methods 0.000 description 4
• RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 4
• 238000011161 development Methods 0.000 description 4
• 230000018109 developmental process Effects 0.000 description 4
• SLPJGDQJLTYWCI-UHFFFAOYSA-N dimethyl-(4,5,6,7-tetrabromo-1h-benzoimidazol-2-yl)-amine Chemical group BrC1=C(Br)C(Br)=C2NC(N(C)C)=NC2=C1Br SLPJGDQJLTYWCI-UHFFFAOYSA-N 0.000 description 4
• 210000002257 embryonic structure Anatomy 0.000 description 4
• 238000002474 experimental method Methods 0.000 description 4
• 102000056513 human GAK Human genes 0.000 description 4
• 102000056545 human NEK2 Human genes 0.000 description 4
• 102000045679 human NMRK2 Human genes 0.000 description 4
• 238000002649 immunization Methods 0.000 description 4
• 238000010166 immunofluorescence Methods 0.000 description 4
• 239000003112 inhibitor Substances 0.000 description 4
• ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 4
• 238000003674 kinase activity assay Methods 0.000 description 4
• 239000007788 liquid Substances 0.000 description 4
• 210000004072 lung Anatomy 0.000 description 4
• 239000012139 lysis buffer Substances 0.000 description 4
• 230000007246 mechanism Effects 0.000 description 4
• 230000001404 mediated effect Effects 0.000 description 4
• BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
• 238000000520 microinjection Methods 0.000 description 4
• 230000017205 mitotic cell cycle checkpoint Effects 0.000 description 4
• 238000010369 molecular cloning Methods 0.000 description 4
• 210000000496 pancreas Anatomy 0.000 description 4
• 230000037361 pathway Effects 0.000 description 4
• BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 4
• 230000036470 plasma concentration Effects 0.000 description 4
• 239000013600 plasmid vector Substances 0.000 description 4
• 210000002307 prostate Anatomy 0.000 description 4
• 239000003531 protein hydrolysate Substances 0.000 description 4
• 125000002294 quinazolinyl group Chemical class N1=C(N=CC2=CC=CC=C12)* 0.000 description 4
• 230000002829 reductive effect Effects 0.000 description 4
• 210000002966 serum Anatomy 0.000 description 4
• 241000894007 species Species 0.000 description 4
• 239000003381 stabilizer Substances 0.000 description 4
• 238000013268 sustained release Methods 0.000 description 4
• 239000012730 sustained-release form Substances 0.000 description 4
• 208000024891 symptom Diseases 0.000 description 4
• 230000001360 synchronised effect Effects 0.000 description 4
• 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 4
• 231100000419 toxicity Toxicity 0.000 description 4
• 230000001988 toxicity Effects 0.000 description 4
• 230000014621 translational initiation Effects 0.000 description 4
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 3
• 229920000936 Agarose Polymers 0.000 description 3
• ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 3
• 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 3
• 101100326430 Caenorhabditis elegans bub-1 gene Proteins 0.000 description 3
• 102000002431 Cyclin G Human genes 0.000 description 3
• 108090000404 Cyclin G1 Proteins 0.000 description 3
• FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 3
• 239000003298 DNA probe Substances 0.000 description 3
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
• 238000009007 Diagnostic Kit Methods 0.000 description 3
• 241001454894 Drosophila heteroneura Species 0.000 description 3
• 241000196324 Embryophyta Species 0.000 description 3
• WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
• 230000010337 G2 phase Effects 0.000 description 3
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
• HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 3
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
• 206010020751 Hypersensitivity Diseases 0.000 description 3
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 3
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
• OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
• 108090000744 Mitogen-Activated Protein Kinase Kinases Proteins 0.000 description 3
• 102000004232 Mitogen-Activated Protein Kinase Kinases Human genes 0.000 description 3
• 241000699666 Mus <mouse, genus> Species 0.000 description 3
• KYRVNWMVYQXFEU-UHFFFAOYSA-N Nocodazole Chemical compound C1=C2NC(NC(=O)OC)=NC2=CC=C1C(=O)C1=CC=CS1 KYRVNWMVYQXFEU-UHFFFAOYSA-N 0.000 description 3
• 239000004677 Nylon Substances 0.000 description 3
• 229910019142 PO4 Inorganic materials 0.000 description 3
• 239000002202 Polyethylene glycol Substances 0.000 description 3
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 3
• 108091081024 Start codon Proteins 0.000 description 3
• 210000001744 T-lymphocyte Anatomy 0.000 description 3
• 108091036066 Three prime untranslated region Proteins 0.000 description 3
• 239000007983 Tris buffer Substances 0.000 description 3
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
• 230000001594 aberrant effect Effects 0.000 description 3
• 239000002671 adjuvant Substances 0.000 description 3
• 239000000443 aerosol Substances 0.000 description 3
• 239000000556 agonist Substances 0.000 description 3
• 235000012538 ammonium bicarbonate Nutrition 0.000 description 3
• 239000001099 ammonium carbonate Substances 0.000 description 3
• 239000005557 antagonist Substances 0.000 description 3
• 229940111121 antirheumatic drug quinolines Drugs 0.000 description 3
• 230000001580 bacterial effect Effects 0.000 description 3
• 239000011324 bead Substances 0.000 description 3
• 210000002459 blastocyst Anatomy 0.000 description 3
• 230000000903 blocking effect Effects 0.000 description 3
• GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 3
• 229910052794 bromium Inorganic materials 0.000 description 3
• 239000001506 calcium phosphate Substances 0.000 description 3
• 229910000389 calcium phosphate Inorganic materials 0.000 description 3
• 235000011010 calcium phosphates Nutrition 0.000 description 3
• 150000001718 carbodiimides Chemical class 0.000 description 3
• 150000001720 carbohydrates Chemical class 0.000 description 3
• 235000014633 carbohydrates Nutrition 0.000 description 3
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
• 210000000170 cell membrane Anatomy 0.000 description 3
• 230000004663 cell proliferation Effects 0.000 description 3
• 238000005119 centrifugation Methods 0.000 description 3
• 210000004720 cerebrum Anatomy 0.000 description 3
• 239000013611 chromosomal DNA Substances 0.000 description 3
• 210000004748 cultured cell Anatomy 0.000 description 3
• 230000007423 decrease Effects 0.000 description 3
• 230000003247 decreasing effect Effects 0.000 description 3
• 239000008298 dragée Substances 0.000 description 3
• 239000003937 drug carrier Substances 0.000 description 3
• 239000000839 emulsion Substances 0.000 description 3
• 150000002148 esters Chemical class 0.000 description 3
• 239000013604 expression vector Substances 0.000 description 3
• 230000004927 fusion Effects 0.000 description 3
• 102000037865 fusion proteins Human genes 0.000 description 3
• 108020001507 fusion proteins Proteins 0.000 description 3
• 239000012362 glacial acetic acid Substances 0.000 description 3
• 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 3
• 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 3
• 210000005260 human cell Anatomy 0.000 description 3
• 238000002513 implantation Methods 0.000 description 3
• 238000000099 in vitro assay Methods 0.000 description 3
• 230000001939 inductive effect Effects 0.000 description 3
• 239000008101 lactose Substances 0.000 description 3
• 210000000265 leukocyte Anatomy 0.000 description 3
• 239000003446 ligand Substances 0.000 description 3
• 210000001165 lymph node Anatomy 0.000 description 3
• 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 3
• MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
• 238000006011 modification reaction Methods 0.000 description 3
• 229950006344 nocodazole Drugs 0.000 description 3
• 229920001778 nylon Polymers 0.000 description 3
• 238000007911 parenteral administration Methods 0.000 description 3
• 239000008188 pellet Substances 0.000 description 3
• 239000012071 phase Substances 0.000 description 3
• 239000010452 phosphate Substances 0.000 description 3
• DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 3
• 210000002826 placenta Anatomy 0.000 description 3
• 230000004481 post-translational protein modification Effects 0.000 description 3
• 239000000843 powder Substances 0.000 description 3
• 230000017854 proteolysis Effects 0.000 description 3
• 150000003248 quinolines Chemical class 0.000 description 3
• 150000003252 quinoxalines Chemical class 0.000 description 3
• 238000003127 radioimmunoassay Methods 0.000 description 3
• 230000010076 replication Effects 0.000 description 3
• 210000002027 skeletal muscle Anatomy 0.000 description 3
• 239000011780 sodium chloride Substances 0.000 description 3
• 239000000600 sorbitol Substances 0.000 description 3
• 210000000952 spleen Anatomy 0.000 description 3
• 238000010561 standard procedure Methods 0.000 description 3
• 235000019698 starch Nutrition 0.000 description 3
• 150000008163 sugars Chemical class 0.000 description 3
• 230000008093 supporting effect Effects 0.000 description 3
• 239000004094 surface-active agent Substances 0.000 description 3
• 239000003826 tablet Substances 0.000 description 3
• CXWXQJXEFPUFDZ-UHFFFAOYSA-N tetralin Chemical compound C1=CC=C2CCCCC2=C1 CXWXQJXEFPUFDZ-UHFFFAOYSA-N 0.000 description 3
• 229940124597 therapeutic agent Drugs 0.000 description 3
• 230000002103 transcriptional effect Effects 0.000 description 3
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 3
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
• IHIXIJGXTJIKRB-UHFFFAOYSA-N trisodium vanadate Chemical compound [Na+].[Na+].[Na+].[O-][V]([O-])([O-])=O IHIXIJGXTJIKRB-UHFFFAOYSA-N 0.000 description 3
• 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 3
• 238000005406 washing Methods 0.000 description 3
• XUHRVZXFBWDCFB-QRTDKPMLSA-N (3R)-4-[[(3S,6S,9S,12R,15S,18R,21R,24R,27R,28R)-12-(3-amino-3-oxopropyl)-6-[(2S)-butan-2-yl]-3-(2-carboxyethyl)-18-(hydroxymethyl)-28-methyl-9,15,21,24-tetrakis(2-methylpropyl)-2,5,8,11,14,17,20,23,26-nonaoxo-1-oxa-4,7,10,13,16,19,22,25-octazacyclooctacos-27-yl]amino]-3-[[(2R)-2-[[(3S)-3-hydroxydecanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoic acid Chemical compound CCCCCCC[C@H](O)CC(=O)N[C@H](CC(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H]1[C@@H](C)OC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CO)NC(=O)[C@@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC1=O)[C@@H](C)CC XUHRVZXFBWDCFB-QRTDKPMLSA-N 0.000 description 2
• 125000003287 1H-imidazol-4-ylmethyl group Chemical group [H]N1C([H])=NC(C([H])([H])[*])=C1[H] 0.000 description 2
• KISWVXRQTGLFGD-UHFFFAOYSA-N 2-[[2-[[6-amino-2-[[2-[[2-[[5-amino-2-[[2-[[1-[2-[[6-amino-2-[(2,5-diamino-5-oxopentanoyl)amino]hexanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-(diaminomethylideneamino)p Chemical compound C1CCN(C(=O)C(CCCN=C(N)N)NC(=O)C(CCCCN)NC(=O)C(N)CCC(N)=O)C1C(=O)NC(CO)C(=O)NC(CCC(N)=O)C(=O)NC(CCCN=C(N)N)C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC(C)C)C(O)=O)CC1=CC=C(O)C=C1 KISWVXRQTGLFGD-UHFFFAOYSA-N 0.000 description 2
• 108020005345 3' Untranslated Regions Proteins 0.000 description 2
• FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
• OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
• 108020003589 5' Untranslated Regions Proteins 0.000 description 2
• FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical group NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 2
• HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
• 229920000178 Acrylic resin Polymers 0.000 description 2
• 239000004925 Acrylic resin Substances 0.000 description 2
• 229930024421 Adenine Natural products 0.000 description 2
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
• 108020004491 Antisense DNA Proteins 0.000 description 2
• 108020005544 Antisense RNA Proteins 0.000 description 2
• 241000972773 Aulopiformes Species 0.000 description 2
• 208000023275 Autoimmune disease Diseases 0.000 description 2
• 108090001008 Avidin Proteins 0.000 description 2
• 101710121713 B1 kinase Proteins 0.000 description 2
• 241000304886 Bacilli Species 0.000 description 2
• 244000063299 Bacillus subtilis Species 0.000 description 2
• 235000014469 Bacillus subtilis Nutrition 0.000 description 2
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
• VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
• 241000282472 Canis lupus familiaris Species 0.000 description 2
• CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
• 208000005623 Carcinogenesis Diseases 0.000 description 2
• 102000011632 Caseins Human genes 0.000 description 2
• 108010076119 Caseins Proteins 0.000 description 2
• 241000700198 Cavia Species 0.000 description 2
• 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 2
• 241000282693 Cercopithecidae Species 0.000 description 2
• ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
• 241000252203 Clupea harengus Species 0.000 description 2
• 102000002427 Cyclin B Human genes 0.000 description 2
• 108010068150 Cyclin B Proteins 0.000 description 2
• 230000004544 DNA amplification Effects 0.000 description 2
• QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical compound CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 2
• 201000003066 Diffuse Scleroderma Diseases 0.000 description 2
• 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 2
• 101100059559 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) nimX gene Proteins 0.000 description 2
• 241000701959 Escherichia virus Lambda Species 0.000 description 2
• 241000282326 Felis catus Species 0.000 description 2
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Chemical group OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
• 239000004471 Glycine Substances 0.000 description 2
• 241001272567 Hominoidea Species 0.000 description 2
• 101100494309 Homo sapiens BUB1 gene Proteins 0.000 description 2
• 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 2
• 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
• MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
• SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
• 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
• GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 2
• 102100025748 Mothers against decapentaplegic homolog 3 Human genes 0.000 description 2
• 101710143111 Mothers against decapentaplegic homolog 3 Proteins 0.000 description 2
• 108010085220 Multiprotein Complexes Proteins 0.000 description 2
• 102000007474 Multiprotein Complexes Human genes 0.000 description 2
• 241000699660 Mus musculus Species 0.000 description 2
• 102000047918 Myelin Basic Human genes 0.000 description 2
• 101710107068 Myelin basic protein Proteins 0.000 description 2
• UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
• 108010094052 Nik related kinase Proteins 0.000 description 2
• 239000000020 Nitrocellulose Substances 0.000 description 2
• BZQFBWGGLXLEPQ-UHFFFAOYSA-N O-phosphoryl-L-serine Natural products OC(=O)C(N)COP(O)(O)=O BZQFBWGGLXLEPQ-UHFFFAOYSA-N 0.000 description 2
• 108700020796 Oncogene Proteins 0.000 description 2
• 238000012408 PCR amplification Methods 0.000 description 2
• 241001494479 Pecora Species 0.000 description 2
• 206010057249 Phagocytosis Diseases 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 2
• 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 2
• OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
• 108010039918 Polylysine Proteins 0.000 description 2
• 102000009516 Protein Serine-Threonine Kinases Human genes 0.000 description 2
• 108010009341 Protein Serine-Threonine Kinases Proteins 0.000 description 2
• 241000589516 Pseudomonas Species 0.000 description 2
• JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
• RADKZDMFGJYCBB-UHFFFAOYSA-N Pyridoxal Chemical compound CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
• SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
• 239000013614 RNA sample Substances 0.000 description 2
• 241000283984 Rodentia Species 0.000 description 2
• 101100465056 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRK1 gene Proteins 0.000 description 2
• 229920002684 Sepharose Polymers 0.000 description 2
• 102100031463 Serine/threonine-protein kinase PLK1 Human genes 0.000 description 2
• 108020004682 Single-Stranded DNA Proteins 0.000 description 2
• 238000002105 Southern blotting Methods 0.000 description 2
• QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
• 206010042953 Systemic sclerosis Diseases 0.000 description 2
• 102100024547 Tensin-1 Human genes 0.000 description 2
• 108010088950 Tensins Proteins 0.000 description 2
• NYTOUQBROMCLBJ-UHFFFAOYSA-N Tetranitromethane Chemical compound [O-][N+](=O)C([N+]([O-])=O)([N+]([O-])=O)[N+]([O-])=O NYTOUQBROMCLBJ-UHFFFAOYSA-N 0.000 description 2
• GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
• 108700019146 Transgenes Proteins 0.000 description 2
• 239000013504 Triton X-100 Substances 0.000 description 2
• 101710162629 Trypsin inhibitor Proteins 0.000 description 2
• 229940122618 Trypsin inhibitor Drugs 0.000 description 2
• 102000004243 Tubulin Human genes 0.000 description 2
• 108090000704 Tubulin Proteins 0.000 description 2
• 241000700618 Vaccinia virus Species 0.000 description 2
• 101100273808 Xenopus laevis cdk1-b gene Proteins 0.000 description 2
• YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
• 230000002378 acidificating effect Effects 0.000 description 2
• 229960000643 adenine Drugs 0.000 description 2
• 210000000577 adipose tissue Anatomy 0.000 description 2
• 230000002411 adverse Effects 0.000 description 2
• 239000011543 agarose gel Substances 0.000 description 2
• 235000010443 alginic acid Nutrition 0.000 description 2
• 239000000783 alginic acid Substances 0.000 description 2
• 229920000615 alginic acid Polymers 0.000 description 2
• 229960001126 alginic acid Drugs 0.000 description 2
• 150000004781 alginic acids Chemical class 0.000 description 2
• 150000001412 amines Chemical class 0.000 description 2
• 230000033115 angiogenesis Effects 0.000 description 2
• 238000010171 animal model Methods 0.000 description 2
• 230000003042 antagnostic effect Effects 0.000 description 2
• 230000002788 anti-peptide Effects 0.000 description 2
• 230000000890 antigenic effect Effects 0.000 description 2
• 239000003816 antisense DNA Substances 0.000 description 2
• 238000013459 approach Methods 0.000 description 2
• 239000007864 aqueous solution Substances 0.000 description 2
• 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 2
• 235000003704 aspartic acid Nutrition 0.000 description 2
• 210000003719 b-lymphocyte Anatomy 0.000 description 2
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
• 230000008827 biological function Effects 0.000 description 2
• 230000033228 biological regulation Effects 0.000 description 2
• 229960002685 biotin Drugs 0.000 description 2
• 235000020958 biotin Nutrition 0.000 description 2
• 239000011616 biotin Substances 0.000 description 2
• 210000001124 body fluid Anatomy 0.000 description 2
• 239000010839 body fluid Substances 0.000 description 2
• 210000001185 bone marrow Anatomy 0.000 description 2
• 210000004556 brain Anatomy 0.000 description 2
• 125000001246 bromo group Chemical group Br* 0.000 description 2
• 239000011575 calcium Substances 0.000 description 2
• 229910052791 calcium Inorganic materials 0.000 description 2
• 230000036952 cancer formation Effects 0.000 description 2
• 231100000504 carcinogenesis Toxicity 0.000 description 2
• 108020001778 catalytic domains Proteins 0.000 description 2
• 238000006555 catalytic reaction Methods 0.000 description 2
• 108700021031 cdc Genes Proteins 0.000 description 2
• 230000012820 cell cycle checkpoint Effects 0.000 description 2
• 230000030833 cell death Effects 0.000 description 2
• 210000003793 centrosome Anatomy 0.000 description 2
• 210000001638 cerebellum Anatomy 0.000 description 2
• 238000012512 characterization method Methods 0.000 description 2
• 239000007795 chemical reaction product Substances 0.000 description 2
• 239000000460 chlorine Substances 0.000 description 2
• 229910052801 chlorine Inorganic materials 0.000 description 2
• 230000002759 chromosomal effect Effects 0.000 description 2
• 230000024321 chromosome segregation Effects 0.000 description 2
• 238000000576 coating method Methods 0.000 description 2
• 238000004040 coloring Methods 0.000 description 2
• 239000003184 complementary RNA Substances 0.000 description 2
• 238000013329 compounding Methods 0.000 description 2
• 238000004132 cross linking Methods 0.000 description 2
• 239000003431 cross linking reagent Substances 0.000 description 2
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
• 230000003111 delayed effect Effects 0.000 description 2
• 238000013461 design Methods 0.000 description 2
• 229950006137 dexfosfoserine Drugs 0.000 description 2
• 239000008121 dextrose Substances 0.000 description 2
• 206010012601 diabetes mellitus Diseases 0.000 description 2
• 230000004069 differentiation Effects 0.000 description 2
• XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
• 230000009977 dual effect Effects 0.000 description 2
• 210000001198 duodenum Anatomy 0.000 description 2
• 239000000975 dye Substances 0.000 description 2
• 235000013601 eggs Nutrition 0.000 description 2
• 238000004520 electroporation Methods 0.000 description 2
• 210000001163 endosome Anatomy 0.000 description 2
• 230000002708 enhancing effect Effects 0.000 description 2
• DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 2
• 238000010195 expression analysis Methods 0.000 description 2
• 239000010685 fatty oil Substances 0.000 description 2
• 239000000945 filler Substances 0.000 description 2
• 235000019253 formic acid Nutrition 0.000 description 2
• 238000013467 fragmentation Methods 0.000 description 2
• 238000006062 fragmentation reaction Methods 0.000 description 2
• 231100000221 frame shift mutation induction Toxicity 0.000 description 2
• 230000037433 frameshift Effects 0.000 description 2
• 239000007789 gas Substances 0.000 description 2
• 239000004220 glutamic acid Chemical group 0.000 description 2
• 235000013922 glutamic acid Nutrition 0.000 description 2
• 230000002414 glycolytic effect Effects 0.000 description 2
• 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
• 230000012010 growth Effects 0.000 description 2
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
• 210000002216 heart Anatomy 0.000 description 2
• 235000019514 herring Nutrition 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 102000046885 human NEK1 Human genes 0.000 description 2
• 210000003917 human chromosome Anatomy 0.000 description 2
• 235000003642 hunger Nutrition 0.000 description 2
• 239000001257 hydrogen Substances 0.000 description 2
• 229910052739 hydrogen Inorganic materials 0.000 description 2
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
• 230000003100 immobilizing effect Effects 0.000 description 2
• 210000002865 immune cell Anatomy 0.000 description 2
• 238000003119 immunoblot Methods 0.000 description 2
• 238000012623 in vivo measurement Methods 0.000 description 2
• 238000012625 in-situ measurement Methods 0.000 description 2
• 230000006698 induction Effects 0.000 description 2
• 208000015181 infectious disease Diseases 0.000 description 2
• 238000001802 infusion Methods 0.000 description 2
• 239000000543 intermediate Substances 0.000 description 2
• 229940043355 kinase inhibitor Drugs 0.000 description 2
• 238000011005 laboratory method Methods 0.000 description 2
• JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
• 239000004816 latex Substances 0.000 description 2
• 229920000126 latex Polymers 0.000 description 2
• GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 2
• 108010052968 leupeptin Proteins 0.000 description 2
• 230000000670 limiting effect Effects 0.000 description 2
• 150000002632 lipids Chemical class 0.000 description 2
• HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
• 210000001161 mammalian embryo Anatomy 0.000 description 2
• 210000005075 mammary gland Anatomy 0.000 description 2
• 239000011159 matrix material Substances 0.000 description 2
• 238000000691 measurement method Methods 0.000 description 2
• 238000000329 molecular dynamics simulation Methods 0.000 description 2
• 210000001616 monocyte Anatomy 0.000 description 2
• 210000004897 n-terminal region Anatomy 0.000 description 2
• 230000000626 neurodegenerative effect Effects 0.000 description 2
• FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 2
• 229920001220 nitrocellulos Polymers 0.000 description 2
• 239000003791 organic solvent mixture Substances 0.000 description 2
• 238000007500 overflow downdraw method Methods 0.000 description 2
• 210000005259 peripheral blood Anatomy 0.000 description 2
• 239000011886 peripheral blood Substances 0.000 description 2
• 230000008782 phagocytosis Effects 0.000 description 2
• WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 2
• OJUGVDODNPJEEC-UHFFFAOYSA-N phenylglyoxal Chemical compound O=CC(=O)C1=CC=CC=C1 OJUGVDODNPJEEC-UHFFFAOYSA-N 0.000 description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
• 230000000865 phosphorylative effect Effects 0.000 description 2
• USRGIUJOYOXOQJ-GBXIJSLDSA-N phosphothreonine Chemical compound OP(=O)(O)O[C@H](C)[C@H](N)C(O)=O USRGIUJOYOXOQJ-GBXIJSLDSA-N 0.000 description 2
• 108010056274 polo-like kinase 1 Proteins 0.000 description 2
• 239000004417 polycarbonate Substances 0.000 description 2
• 229920000515 polycarbonate Polymers 0.000 description 2
• 229920000656 polylysine Polymers 0.000 description 2
• 108091033319 polynucleotide Proteins 0.000 description 2
• 102000040430 polynucleotide Human genes 0.000 description 2
• 239000002157 polynucleotide Substances 0.000 description 2
• 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 2
• 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
• 229940068968 polysorbate 80 Drugs 0.000 description 2
• 229920000053 polysorbate 80 Polymers 0.000 description 2
• 238000001556 precipitation Methods 0.000 description 2
• 150000003141 primary amines Chemical class 0.000 description 2
• 239000003380 propellant Substances 0.000 description 2
• 239000003909 protein kinase inhibitor Substances 0.000 description 2
• NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 2
• 239000011541 reaction mixture Substances 0.000 description 2
• 230000008707 rearrangement Effects 0.000 description 2
• 230000006798 recombination Effects 0.000 description 2
• 238000005215 recombination Methods 0.000 description 2
• 230000022983 regulation of cell cycle Effects 0.000 description 2
• 230000004044 response Effects 0.000 description 2
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
• 210000003079 salivary gland Anatomy 0.000 description 2
• 235000019515 salmon Nutrition 0.000 description 2
• 210000000582 semen Anatomy 0.000 description 2
• 230000007781 signaling event Effects 0.000 description 2
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
• 230000000087 stabilizing effect Effects 0.000 description 2
• 239000008107 starch Substances 0.000 description 2
• 210000002784 stomach Anatomy 0.000 description 2
• 125000005504 styryl group Chemical group 0.000 description 2
• 238000007920 subcutaneous administration Methods 0.000 description 2
• 239000000454 talc Substances 0.000 description 2
• 229910052623 talc Inorganic materials 0.000 description 2
• 235000012222 talc Nutrition 0.000 description 2
• 230000008685 targeting Effects 0.000 description 2
• 210000001550 testis Anatomy 0.000 description 2
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
• 230000005026 transcription initiation Effects 0.000 description 2
• 230000009466 transformation Effects 0.000 description 2
• 238000011426 transformation method Methods 0.000 description 2
• 230000010474 transient expression Effects 0.000 description 2
• 239000002753 trypsin inhibitor Substances 0.000 description 2
• 229940121358 tyrosine kinase inhibitor Drugs 0.000 description 2
• 241001529453 unidentified herpesvirus Species 0.000 description 2
• 241001515965 unidentified phage Species 0.000 description 2
• 241001430294 unidentified retrovirus Species 0.000 description 2
• 210000003932 urinary bladder Anatomy 0.000 description 2
• 210000004291 uterus Anatomy 0.000 description 2
• 239000003981 vehicle Substances 0.000 description 2
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
• LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 description 1
• BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
• NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
• DDMOUSALMHHKOS-UHFFFAOYSA-N 1,2-dichloro-1,1,2,2-tetrafluoroethane Chemical compound FC(F)(Cl)C(F)(F)Cl DDMOUSALMHHKOS-UHFFFAOYSA-N 0.000 description 1
• IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
• NHJVRSWLHSJWIN-UHFFFAOYSA-N 2,4,6-trinitrobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O NHJVRSWLHSJWIN-UHFFFAOYSA-N 0.000 description 1
• 150000003923 2,5-pyrrolediones Chemical class 0.000 description 1
• BHANCCMWYDZQOR-UHFFFAOYSA-N 2-(methyldisulfanyl)pyridine Chemical compound CSSC1=CC=CC=N1 BHANCCMWYDZQOR-UHFFFAOYSA-N 0.000 description 1
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
• FKJSFKCZZIXQIP-UHFFFAOYSA-N 2-bromo-1-(4-bromophenyl)ethanone Chemical compound BrCC(=O)C1=CC=C(Br)C=C1 FKJSFKCZZIXQIP-UHFFFAOYSA-N 0.000 description 1
• NKDFYOWSKOHCCO-YPVLXUMRSA-N 20-hydroxyecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@](C)(O)[C@H](O)CCC(C)(O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 NKDFYOWSKOHCCO-YPVLXUMRSA-N 0.000 description 1
• RYCSNJCJOVQGBC-UHFFFAOYSA-N 3-(1-cyclopropyl-2h-pyridin-4-yl)-1h-quinolin-2-one Chemical class O=C1NC2=CC=CC=C2C=C1C(C=C1)=CCN1C1CC1 RYCSNJCJOVQGBC-UHFFFAOYSA-N 0.000 description 1
• BIGBDMFRWJRLGJ-UHFFFAOYSA-N 3-benzyl-1,5-didiazoniopenta-1,4-diene-2,4-diolate Chemical compound [N-]=[N+]=CC(=O)C(C(=O)C=[N+]=[N-])CC1=CC=CC=C1 BIGBDMFRWJRLGJ-UHFFFAOYSA-N 0.000 description 1
• ONZQYZKCUHFORE-UHFFFAOYSA-N 3-bromo-1,1,1-trifluoropropan-2-one Chemical compound FC(F)(F)C(=O)CBr ONZQYZKCUHFORE-UHFFFAOYSA-N 0.000 description 1
• QHSXWDVVFHXHHB-UHFFFAOYSA-N 3-nitro-2-[(3-nitropyridin-2-yl)disulfanyl]pyridine Chemical compound [O-][N+](=O)C1=CC=CN=C1SSC1=NC=CC=C1[N+]([O-])=O QHSXWDVVFHXHHB-UHFFFAOYSA-N 0.000 description 1
• NLPWSMKACWGINL-UHFFFAOYSA-N 4-azido-2-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(N=[N+]=[N-])C=C1O NLPWSMKACWGINL-UHFFFAOYSA-N 0.000 description 1
• 102100022681 40S ribosomal protein S27 Human genes 0.000 description 1
• WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 1
• 244000215068 Acacia senegal Species 0.000 description 1
• 241000203809 Actinomycetales Species 0.000 description 1
• 241000251468 Actinopterygii Species 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 229920001817 Agar Polymers 0.000 description 1
• GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
• 201000000541 Ambras type hypertrichosis universalis congenita Diseases 0.000 description 1
• QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 241000228212 Aspergillus Species 0.000 description 1
• 206010003497 Asphyxia Diseases 0.000 description 1
• 241000416162 Astragalus gummifer Species 0.000 description 1
• 108090000461 Aurora Kinase A Proteins 0.000 description 1
• 102100032311 Aurora kinase A Human genes 0.000 description 1
• 102100032306 Aurora kinase B Human genes 0.000 description 1
• 108090000749 Aurora kinase B Proteins 0.000 description 1
• WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 description 1
• 101150021818 BUB2 gene Proteins 0.000 description 1
• 241000193830 Bacillus <bacterium> Species 0.000 description 1
• 101900315840 Bacillus subtilis Alpha-amylase Proteins 0.000 description 1
• 102100026189 Beta-galactosidase Human genes 0.000 description 1
• 241000255789 Bombyx mori Species 0.000 description 1
• 241000283690 Bos taurus Species 0.000 description 1
• WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
• 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
• 208000004434 Calcinosis Diseases 0.000 description 1
• 108010026870 Calcium-Calmodulin-Dependent Protein Kinases Proteins 0.000 description 1
• 102000019025 Calcium-Calmodulin-Dependent Protein Kinases Human genes 0.000 description 1
• 102000014914 Carrier Proteins Human genes 0.000 description 1
• 102000053642 Catalytic RNA Human genes 0.000 description 1
• 108090000994 Catalytic RNA Proteins 0.000 description 1
• 241000701489 Cauliflower mosaic virus Species 0.000 description 1
• 102000011068 Cdc42 Human genes 0.000 description 1
• 108050001278 Cdc42 Proteins 0.000 description 1
• 102000005483 Cell Cycle Proteins Human genes 0.000 description 1
• 108010031896 Cell Cycle Proteins Proteins 0.000 description 1
• 235000021538 Chard Nutrition 0.000 description 1
• 101000709520 Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) Atypical response regulator protein ChxR Proteins 0.000 description 1
• 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
• 108010077544 Chromatin Proteins 0.000 description 1
• 206010008805 Chromosomal abnormalities Diseases 0.000 description 1
• 208000031404 Chromosome Aberrations Diseases 0.000 description 1
• ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
• 102000008186 Collagen Human genes 0.000 description 1
• 108010035532 Collagen Proteins 0.000 description 1
• 108010047041 Complementarity Determining Regions Proteins 0.000 description 1
• RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
• 229920002261 Corn starch Polymers 0.000 description 1
• 239000004971 Cross linker Substances 0.000 description 1
• 108010068192 Cyclin A Proteins 0.000 description 1
• 102100025191 Cyclin-A2 Human genes 0.000 description 1
• 102100032857 Cyclin-dependent kinase 1 Human genes 0.000 description 1
• 101710106279 Cyclin-dependent kinase 1 Proteins 0.000 description 1
• 108090000266 Cyclin-dependent kinases Proteins 0.000 description 1
• 102000003903 Cyclin-dependent kinases Human genes 0.000 description 1
• 241000701022 Cytomegalovirus Species 0.000 description 1
• FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
• FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
• 108020003215 DNA Probes Proteins 0.000 description 1
• 230000004543 DNA replication Effects 0.000 description 1
• 238000001712 DNA sequencing Methods 0.000 description 1
• 241000537219 Deltabaculovirus Species 0.000 description 1
• 241000702421 Dependoparvovirus Species 0.000 description 1
• 229920002307 Dextran Polymers 0.000 description 1
• FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
• 239000004338 Dichlorodifluoromethane Substances 0.000 description 1
• 108090000204 Dipeptidase 1 Proteins 0.000 description 1
• 108700010895 Drosophila ADH Proteins 0.000 description 1
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
• LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
• 241000792859 Enema Species 0.000 description 1
• 241000283086 Equidae Species 0.000 description 1
• 208000004248 Familial Primary Pulmonary Hypertension Diseases 0.000 description 1
• 206010016654 Fibrosis Diseases 0.000 description 1
• 241000233866 Fungi Species 0.000 description 1
• 230000010190 G1 phase Effects 0.000 description 1
• 230000004707 G1/S transition Effects 0.000 description 1
• 101150082479 GAL gene Proteins 0.000 description 1
• 101150027701 Gak gene Proteins 0.000 description 1
• 108010001515 Galectin 4 Proteins 0.000 description 1
• 102000006395 Globulins Human genes 0.000 description 1
• 108010044091 Globulins Proteins 0.000 description 1
• SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
• 108010070675 Glutathione transferase Proteins 0.000 description 1
• 229920000084 Gum arabic Polymers 0.000 description 1
• 238000010268 HPLC based assay Methods 0.000 description 1
• 239000012981 Hank's balanced salt solution Substances 0.000 description 1
• 101000678466 Homo sapiens 40S ribosomal protein S27 Proteins 0.000 description 1
• 101001019502 Homo sapiens Alpha-L-iduronidase Proteins 0.000 description 1
• 101000659223 Homo sapiens Dual specificity protein kinase TTK Proteins 0.000 description 1
• 101000871017 Homo sapiens Growth factor receptor-bound protein 2 Proteins 0.000 description 1
• 101001078385 Homo sapiens Haptoglobin Proteins 0.000 description 1
• 101000962483 Homo sapiens Max dimerization protein 1 Proteins 0.000 description 1
• 101001000302 Homo sapiens Max-interacting protein 1 Proteins 0.000 description 1
• 101000896484 Homo sapiens Mitotic checkpoint protein BUB3 Proteins 0.000 description 1
• 101000957106 Homo sapiens Mitotic spindle assembly checkpoint protein MAD1 Proteins 0.000 description 1
• 101000957259 Homo sapiens Mitotic spindle assembly checkpoint protein MAD2A Proteins 0.000 description 1
• 101100516354 Homo sapiens NEK4 gene Proteins 0.000 description 1
• 101000692455 Homo sapiens Platelet-derived growth factor receptor beta Proteins 0.000 description 1
• 101000897979 Homo sapiens Putative spermatid-specific linker histone H1-like protein Proteins 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical group OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
• 102100020870 La-related protein 6 Human genes 0.000 description 1
• 108050008265 La-related protein 6 Proteins 0.000 description 1
• 102100021922 Low-density lipoprotein receptor-related protein 2 Human genes 0.000 description 1
• 229930195725 Mannitol Natural products 0.000 description 1
• 102100039185 Max dimerization protein 1 Human genes 0.000 description 1
• 102100021718 Mitotic checkpoint protein BUB3 Human genes 0.000 description 1
• 102100038792 Mitotic spindle assembly checkpoint protein MAD2A Human genes 0.000 description 1
• 101000590284 Mus musculus 26S proteasome non-ATPase regulatory subunit 14 Proteins 0.000 description 1
• 101000969137 Mus musculus Metallothionein-1 Proteins 0.000 description 1
• 102000003505 Myosin Human genes 0.000 description 1
• 108060008487 Myosin Proteins 0.000 description 1
• NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical class ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
• 125000000729 N-terminal amino-acid group Chemical group 0.000 description 1
• 101150072440 NEK4 gene Proteins 0.000 description 1
• 108091061960 Naked DNA Proteins 0.000 description 1
• 229930193140 Neomycin Natural products 0.000 description 1
• 208000009869 Neu-Laxova syndrome Diseases 0.000 description 1
• 206010029260 Neuroblastoma Diseases 0.000 description 1
• 108091092724 Noncoding DNA Proteins 0.000 description 1
• 108010077850 Nuclear Localization Signals Proteins 0.000 description 1
• LTBRACVJRXLQHC-UHFFFAOYSA-N OP(=O)OCC1=CC=CC=C1 Chemical class OP(=O)OCC1=CC=CC=C1 LTBRACVJRXLQHC-UHFFFAOYSA-N 0.000 description 1
• 102100026547 Platelet-derived growth factor receptor beta Human genes 0.000 description 1
• 101710182846 Polyhedrin Proteins 0.000 description 1
• 229920002642 Polysorbate 65 Polymers 0.000 description 1
• 241000288906 Primates Species 0.000 description 1
• 201000004681 Psoriasis Diseases 0.000 description 1
• 206010064911 Pulmonary arterial hypertension Diseases 0.000 description 1
• 102100021861 Putative spermatid-specific linker histone H1-like protein Human genes 0.000 description 1
• WTKZEGDFNFYCGP-UHFFFAOYSA-N Pyrazole Chemical compound C=1C=NNC=1 WTKZEGDFNFYCGP-UHFFFAOYSA-N 0.000 description 1
• 108020004518 RNA Probes Proteins 0.000 description 1
• 239000003391 RNA probe Substances 0.000 description 1
• 230000006819 RNA synthesis Effects 0.000 description 1
• 101100228161 Rattus norvegicus Gak gene Proteins 0.000 description 1
• 208000012322 Raynaud phenomenon Diseases 0.000 description 1
• 108090000873 Receptor Protein-Tyrosine Kinases Proteins 0.000 description 1
• 102000004278 Receptor Protein-Tyrosine Kinases Human genes 0.000 description 1
• 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
• 101150061026 SDI1 gene Proteins 0.000 description 1
• 102000000395 SH3 domains Human genes 0.000 description 1
• 108050008861 SH3 domains Proteins 0.000 description 1
• 241000235070 Saccharomyces Species 0.000 description 1
• 101100056290 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ARK1 gene Proteins 0.000 description 1
• 241000607142 Salmonella Species 0.000 description 1
• 239000002262 Schiff base Substances 0.000 description 1
• 150000004753 Schiff bases Chemical class 0.000 description 1
• 101100327314 Schizosaccharomyces pombe (strain 972 / ATCC 24843) cdc16 gene Proteins 0.000 description 1
• 238000012300 Sequence Analysis Methods 0.000 description 1
• 241000607720 Serratia Species 0.000 description 1
• 108050007496 Shikimate kinase 2 Proteins 0.000 description 1
• 102220497176 Small vasohibin-binding protein_T47D_mutation Human genes 0.000 description 1
• 229920002125 Sokalan® Polymers 0.000 description 1
• 101001060868 Strawberry mild yellow edge-associated virus Helicase Proteins 0.000 description 1
• 108010090804 Streptavidin Proteins 0.000 description 1
• KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
• 229930006000 Sucrose Natural products 0.000 description 1
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
• 241000282887 Suidae Species 0.000 description 1
• 201000009594 Systemic Scleroderma Diseases 0.000 description 1
• 108700026226 TATA Box Proteins 0.000 description 1
• 238000012288 TUNEL assay Methods 0.000 description 1
• FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
• 206010043189 Telangiectasia Diseases 0.000 description 1
• 108020005038 Terminator Codon Proteins 0.000 description 1
• 208000035199 Tetraploidy Diseases 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
• 101710120037 Toxin CcdB Proteins 0.000 description 1
• 229920001615 Tragacanth Polymers 0.000 description 1
• 108091023040 Transcription factor Proteins 0.000 description 1
• 102000040945 Transcription factor Human genes 0.000 description 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
• 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
• 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
• 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
• 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
• XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
• 208000036142 Viral infection Diseases 0.000 description 1
• 206010052428 Wound Diseases 0.000 description 1
• 208000027418 Wounds and injury Diseases 0.000 description 1
• 101000752225 Xenopus laevis Low density lipoprotein receptor adapter protein 1-A Proteins 0.000 description 1
• 241000607479 Yersinia pestis Species 0.000 description 1
• KYIKRXIYLAGAKQ-UHFFFAOYSA-N abcn Chemical compound C1CCCCC1(C#N)N=NC1(C#N)CCCCC1 KYIKRXIYLAGAKQ-UHFFFAOYSA-N 0.000 description 1
• 235000010489 acacia gum Nutrition 0.000 description 1
• 239000000205 acacia gum Substances 0.000 description 1
• 235000011054 acetic acid Nutrition 0.000 description 1
• DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
• 238000006640 acetylation reaction Methods 0.000 description 1
• 238000005903 acid hydrolysis reaction Methods 0.000 description 1
• 150000007513 acids Chemical class 0.000 description 1
• 230000003213 activating effect Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 238000001042 affinity chromatography Methods 0.000 description 1
• 239000007801 affinity label Substances 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 239000008272 agar Substances 0.000 description 1
• 230000032683 aging Effects 0.000 description 1
• 238000013019 agitation Methods 0.000 description 1
• 230000001270 agonistic effect Effects 0.000 description 1
• 125000000217 alkyl group Chemical group 0.000 description 1
• 208000026935 allergic disease Diseases 0.000 description 1
• BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
• 230000004075 alteration Effects 0.000 description 1
• 238000007112 amidation reaction Methods 0.000 description 1
• 230000006229 amino acid addition Effects 0.000 description 1
• 125000003277 amino group Chemical group 0.000 description 1
• 230000031016 anaphase Effects 0.000 description 1
• 230000003322 aneuploid effect Effects 0.000 description 1
• 208000036878 aneuploidy Diseases 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• 239000002246 antineoplastic agent Substances 0.000 description 1
• 229940041181 antineoplastic drug Drugs 0.000 description 1
• 229940045988 antineoplastic drug protein kinase inhibitors Drugs 0.000 description 1
• 238000003782 apoptosis assay Methods 0.000 description 1
• 230000005775 apoptotic pathway Effects 0.000 description 1
• 239000008346 aqueous phase Substances 0.000 description 1
• 239000008135 aqueous vehicle Substances 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 1
• 125000004429 atom Chemical group 0.000 description 1
• 239000013602 bacteriophage vector Substances 0.000 description 1
• 230000004888 barrier function Effects 0.000 description 1
• 208000036815 beta tubulin Diseases 0.000 description 1
• 108010005774 beta-Galactosidase Proteins 0.000 description 1
• 125000002619 bicyclic group Chemical group 0.000 description 1
• 230000001588 bifunctional effect Effects 0.000 description 1
• 239000011230 binding agent Substances 0.000 description 1
• 108091008324 binding proteins Proteins 0.000 description 1
• 238000004166 bioassay Methods 0.000 description 1
• 229920000249 biocompatible polymer Polymers 0.000 description 1
• 230000004071 biological effect Effects 0.000 description 1
• 230000031018 biological processes and functions Effects 0.000 description 1
• 230000005540 biological transmission Effects 0.000 description 1
• 238000009835 boiling Methods 0.000 description 1
• 229940098773 bovine serum albumin Drugs 0.000 description 1
• 229950004398 broxuridine Drugs 0.000 description 1
• KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
• 229910000019 calcium carbonate Inorganic materials 0.000 description 1
• 238000004422 calculation algorithm Methods 0.000 description 1
• 239000001569 carbon dioxide Substances 0.000 description 1
• 229910002092 carbon dioxide Inorganic materials 0.000 description 1
• 229960004424 carbon dioxide Drugs 0.000 description 1
• 239000001768 carboxy methyl cellulose Substances 0.000 description 1
• 150000001244 carboxylic acid anhydrides Chemical class 0.000 description 1
• 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 1
• 230000015556 catabolic process Effects 0.000 description 1
• 239000006143 cell culture medium Substances 0.000 description 1
• 230000006369 cell cycle progression Effects 0.000 description 1
• 230000003833 cell viability Effects 0.000 description 1
• 210000002421 cell wall Anatomy 0.000 description 1
• 230000030570 cellular localization Effects 0.000 description 1
• 230000033077 cellular process Effects 0.000 description 1
• 230000036755 cellular response Effects 0.000 description 1
• 230000005754 cellular signaling Effects 0.000 description 1
• 210000003169 central nervous system Anatomy 0.000 description 1
• 238000002512 chemotherapy Methods 0.000 description 1
• VIMWCINSBRXAQH-UHFFFAOYSA-M chloro-(2-hydroxy-5-nitrophenyl)mercury Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[Hg]Cl VIMWCINSBRXAQH-UHFFFAOYSA-M 0.000 description 1
• VXIVSQZSERGHQP-UHFFFAOYSA-N chloroacetamide Chemical compound NC(=O)CCl VXIVSQZSERGHQP-UHFFFAOYSA-N 0.000 description 1
• FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
• 229940106681 chloroacetic acid Drugs 0.000 description 1
• 210000003483 chromatin Anatomy 0.000 description 1
• 230000014107 chromosome localization Effects 0.000 description 1
• 238000003776 cleavage reaction Methods 0.000 description 1
• 238000012411 cloning technique Methods 0.000 description 1
• 229940110456 cocoa butter Drugs 0.000 description 1
• 235000019868 cocoa butter Nutrition 0.000 description 1
• ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
• 235000017471 coenzyme Q10 Nutrition 0.000 description 1
• 229920001436 collagen Polymers 0.000 description 1
• 238000002648 combination therapy Methods 0.000 description 1
• 230000000536 complexating effect Effects 0.000 description 1
• 238000010668 complexation reaction Methods 0.000 description 1
• 238000004590 computer program Methods 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 235000008504 concentrate Nutrition 0.000 description 1
• 230000021615 conjugation Effects 0.000 description 1
• 238000011109 contamination Methods 0.000 description 1
• 230000008602 contraction Effects 0.000 description 1
• 230000001276 controlling effect Effects 0.000 description 1
• 238000007796 conventional method Methods 0.000 description 1
• 229910052802 copper Inorganic materials 0.000 description 1
• 239000010949 copper Substances 0.000 description 1
• 239000008120 corn starch Substances 0.000 description 1
• 229940099112 cornstarch Drugs 0.000 description 1
• 230000008878 coupling Effects 0.000 description 1
• 238000010168 coupling process Methods 0.000 description 1
• 238000005859 coupling reaction Methods 0.000 description 1
• 238000012258 culturing Methods 0.000 description 1
• 210000001771 cumulus cell Anatomy 0.000 description 1
• 238000005520 cutting process Methods 0.000 description 1
• ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
• 125000004122 cyclic group Chemical group 0.000 description 1
• 210000000805 cytoplasm Anatomy 0.000 description 1
• 229940104302 cytosine Drugs 0.000 description 1
• 231100000433 cytotoxic Toxicity 0.000 description 1
• 230000001472 cytotoxic effect Effects 0.000 description 1
• 230000002950 deficient Effects 0.000 description 1
• 230000006735 deficit Effects 0.000 description 1
• 230000007850 degeneration Effects 0.000 description 1
• 238000006731 degradation reaction Methods 0.000 description 1
• 230000002939 deleterious effect Effects 0.000 description 1
• 238000004925 denaturation Methods 0.000 description 1
• 230000036425 denaturation Effects 0.000 description 1
• 210000004443 dendritic cell Anatomy 0.000 description 1
• 238000009795 derivation Methods 0.000 description 1
• 239000003599 detergent Substances 0.000 description 1
• 229960002086 dextran Drugs 0.000 description 1
• 229960000633 dextran sulfate Drugs 0.000 description 1
• PXBRQCKWGAHEHS-UHFFFAOYSA-N dichlorodifluoromethane Chemical compound FC(F)(Cl)Cl PXBRQCKWGAHEHS-UHFFFAOYSA-N 0.000 description 1
• 235000019404 dichlorodifluoromethane Nutrition 0.000 description 1
• 229940042935 dichlorodifluoromethane Drugs 0.000 description 1
• 229940087091 dichlorotetrafluoroethane Drugs 0.000 description 1
• 235000014113 dietary fatty acids Nutrition 0.000 description 1
• 238000009792 diffusion process Methods 0.000 description 1
• 208000037765 diseases and disorders Diseases 0.000 description 1
• 239000002270 dispersing agent Substances 0.000 description 1
• 238000012377 drug delivery Methods 0.000 description 1
• 241001493065 dsRNA viruses Species 0.000 description 1
• 101150026638 eca gene Proteins 0.000 description 1
• 239000012636 effector Substances 0.000 description 1
• 230000005684 electric field Effects 0.000 description 1
• 238000001962 electrophoresis Methods 0.000 description 1
• 238000010828 elution Methods 0.000 description 1
• 210000002308 embryonic cell Anatomy 0.000 description 1
• 210000001671 embryonic stem cell Anatomy 0.000 description 1
• 230000001804 emulsifying effect Effects 0.000 description 1
• 239000007920 enema Substances 0.000 description 1
• 229940079360 enema for constipation Drugs 0.000 description 1
• 239000003623 enhancer Substances 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 150000002170 ethers Chemical group 0.000 description 1
• LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
• 229940093471 ethyl oleate Drugs 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 229930195729 fatty acid Natural products 0.000 description 1
• 239000000194 fatty acid Substances 0.000 description 1
• 150000004665 fatty acids Chemical class 0.000 description 1
• 210000004420 female germ cell Anatomy 0.000 description 1
• 239000012091 fetal bovine serum Substances 0.000 description 1
• 210000003754 fetus Anatomy 0.000 description 1
• 210000002950 fibroblast Anatomy 0.000 description 1
• 230000004761 fibrosis Effects 0.000 description 1
• 230000003176 fibrotic effect Effects 0.000 description 1
• 239000013020 final formulation Substances 0.000 description 1
• 235000019688 fish Nutrition 0.000 description 1
• 238000000684 flow cytometry Methods 0.000 description 1
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 238000001215 fluorescent labelling Methods 0.000 description 1
• 239000011888 foil Substances 0.000 description 1
• 230000037406 food intake Effects 0.000 description 1
• 238000004108 freeze drying Methods 0.000 description 1
• 230000005714 functional activity Effects 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• IRSCQMHQWWYFCW-UHFFFAOYSA-N ganciclovir Chemical compound O=C1NC(N)=NC2=C1N=CN2COC(CO)CO IRSCQMHQWWYFCW-UHFFFAOYSA-N 0.000 description 1
• 229960002963 ganciclovir Drugs 0.000 description 1
• 210000000609 ganglia Anatomy 0.000 description 1
• 238000010353 genetic engineering Methods 0.000 description 1
• 230000037442 genomic alteration Effects 0.000 description 1
• 210000004602 germ cell Anatomy 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
• 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 1
• 125000005456 glyceride group Chemical group 0.000 description 1
• 230000013595 glycosylation Effects 0.000 description 1
• 238000006206 glycosylation reaction Methods 0.000 description 1
• HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 1
• ZRALSGWEFCBTJO-UHFFFAOYSA-N guanidine group Chemical group NC(=N)N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
• 150000002357 guanidines Chemical class 0.000 description 1
• 230000035876 healing Effects 0.000 description 1
• 229910001385 heavy metal Inorganic materials 0.000 description 1
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
• 125000000623 heterocyclic group Chemical group 0.000 description 1
• 102000034345 heterotrimeric G proteins Human genes 0.000 description 1
• 108091006093 heterotrimeric G proteins Proteins 0.000 description 1
• 238000013537 high throughput screening Methods 0.000 description 1
• 210000003630 histaminocyte Anatomy 0.000 description 1
• 238000002744 homologous recombination Methods 0.000 description 1
• 230000006801 homologous recombination Effects 0.000 description 1
• 102000050796 human HP Human genes 0.000 description 1
• 229920001600 hydrophobic polymer Polymers 0.000 description 1
• 230000033444 hydroxylation Effects 0.000 description 1
• 238000005805 hydroxylation reaction Methods 0.000 description 1
• 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
• 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
• 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
• UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 1
• 230000009610 hypersensitivity Effects 0.000 description 1
• 230000001900 immune effect Effects 0.000 description 1
• 230000028993 immune response Effects 0.000 description 1
• 210000000987 immune system Anatomy 0.000 description 1
• 238000003317 immunochromatography Methods 0.000 description 1
• 238000003365 immunocytochemistry Methods 0.000 description 1
• 230000009851 immunogenic response Effects 0.000 description 1
• 230000002621 immunoprecipitating effect Effects 0.000 description 1
• 238000001114 immunoprecipitation Methods 0.000 description 1
• 230000001976 improved effect Effects 0.000 description 1
• 238000007901 in situ hybridization Methods 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• 238000011534 incubation Methods 0.000 description 1
• PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
• RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 229910001410 inorganic ion Inorganic materials 0.000 description 1
• 238000003780 insertion Methods 0.000 description 1
• 230000037431 insertion Effects 0.000 description 1
• 230000016507 interphase Effects 0.000 description 1
• 230000000968 intestinal effect Effects 0.000 description 1
• 238000007918 intramuscular administration Methods 0.000 description 1
• 239000007927 intramuscular injection Substances 0.000 description 1
• 238000010255 intramuscular injection Methods 0.000 description 1
• 238000007912 intraperitoneal administration Methods 0.000 description 1
• 239000007928 intraperitoneal injection Substances 0.000 description 1
• 238000007913 intrathecal administration Methods 0.000 description 1
• 238000001990 intravenous administration Methods 0.000 description 1
• 238000007914 intraventricular administration Methods 0.000 description 1
• PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
• 239000003456 ion exchange resin Substances 0.000 description 1
• 229920003303 ion-exchange polymer Polymers 0.000 description 1
• 230000000155 isotopic effect Effects 0.000 description 1
• 210000002415 kinetochore Anatomy 0.000 description 1
• 101150109249 lacI gene Proteins 0.000 description 1
• 101150066555 lacZ gene Proteins 0.000 description 1
• 239000004922 lacquer Substances 0.000 description 1
• 239000004310 lactic acid Substances 0.000 description 1
• 235000014655 lactic acid Nutrition 0.000 description 1
• 229940057995 liquid paraffin Drugs 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 231100000053 low toxicity Toxicity 0.000 description 1
• 239000007937 lozenge Substances 0.000 description 1
• 239000000314 lubricant Substances 0.000 description 1
• 235000019359 magnesium stearate Nutrition 0.000 description 1
• 230000005291 magnetic effect Effects 0.000 description 1
• 230000014759 maintenance of location Effects 0.000 description 1
• 210000003794 male germ cell Anatomy 0.000 description 1
• 239000001630 malic acid Substances 0.000 description 1
• 235000011090 malic acid Nutrition 0.000 description 1
• 230000003211 malignant effect Effects 0.000 description 1
• 239000000594 mannitol Substances 0.000 description 1
• 235000010355 mannitol Nutrition 0.000 description 1
• 239000003550 marker Substances 0.000 description 1
• 230000035800 maturation Effects 0.000 description 1
• 210000003593 megakaryocyte Anatomy 0.000 description 1
• 230000031355 meiotic cell cycle Effects 0.000 description 1
• 102000006240 membrane receptors Human genes 0.000 description 1
• 108020004084 membrane receptors Proteins 0.000 description 1
• 239000002207 metabolite Substances 0.000 description 1
• 229910052751 metal Inorganic materials 0.000 description 1
• 239000002184 metal Substances 0.000 description 1
• 229920000609 methyl cellulose Polymers 0.000 description 1
• NEGQCMNHXHSFGU-UHFFFAOYSA-N methyl pyridine-2-carboximidate Chemical compound COC(=N)C1=CC=CC=N1 NEGQCMNHXHSFGU-UHFFFAOYSA-N 0.000 description 1
• 238000007069 methylation reaction Methods 0.000 description 1
• 239000001923 methylcellulose Substances 0.000 description 1
• 235000010981 methylcellulose Nutrition 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 239000003226 mitogen Substances 0.000 description 1
• 230000036456 mitotic arrest Effects 0.000 description 1
• 108091005573 modified proteins Proteins 0.000 description 1
• 102000035118 modified proteins Human genes 0.000 description 1
• 238000012544 monitoring process Methods 0.000 description 1
• 125000002950 monocyclic group Chemical group 0.000 description 1
• 125000004312 morpholin-2-yl group Chemical group [H]N1C([H])([H])C([H])([H])OC([H])(*)C1([H])[H] 0.000 description 1
• 238000002887 multiple sequence alignment Methods 0.000 description 1
• 230000000869 mutational effect Effects 0.000 description 1
• 230000014399 negative regulation of angiogenesis Effects 0.000 description 1
• 101150063132 nek2 gene Proteins 0.000 description 1
• 229960004927 neomycin Drugs 0.000 description 1
• 210000005036 nerve Anatomy 0.000 description 1
• 210000003757 neuroblast Anatomy 0.000 description 1
• 230000004770 neurodegeneration Effects 0.000 description 1
• 210000002569 neuron Anatomy 0.000 description 1
• 230000003472 neutralizing effect Effects 0.000 description 1
• 230000009871 nonspecific binding Effects 0.000 description 1
• 231100000252 nontoxic Toxicity 0.000 description 1
• 230000003000 nontoxic effect Effects 0.000 description 1
• 231100000956 nontoxicity Toxicity 0.000 description 1
• 108010058731 nopaline synthase Proteins 0.000 description 1
• 238000012758 nuclear staining Methods 0.000 description 1
• 238000011580 nude mouse model Methods 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 235000019198 oils Nutrition 0.000 description 1
• 238000011275 oncology therapy Methods 0.000 description 1
• 210000000287 oocyte Anatomy 0.000 description 1
• 238000005457 optimization Methods 0.000 description 1
• 150000002894 organic compounds Chemical class 0.000 description 1
• 229920000620 organic polymer Polymers 0.000 description 1
• 239000003960 organic solvent Substances 0.000 description 1
• 230000002611 ovarian Effects 0.000 description 1
• 210000003101 oviduct Anatomy 0.000 description 1
• YFZOUMNUDGGHIW-UHFFFAOYSA-M p-chloromercuribenzoic acid Chemical compound OC(=O)C1=CC=C([Hg]Cl)C=C1 YFZOUMNUDGGHIW-UHFFFAOYSA-M 0.000 description 1
• 108010058266 p21-Activated Kinases Proteins 0.000 description 1
• 102000006271 p21-Activated Kinases Human genes 0.000 description 1
• 238000004806 packaging method and process Methods 0.000 description 1
• 230000005298 paramagnetic effect Effects 0.000 description 1
• 239000013618 particulate matter Substances 0.000 description 1
• 230000002093 peripheral effect Effects 0.000 description 1
• 239000000825 pharmaceutical preparation Substances 0.000 description 1
• 239000008363 phosphate buffer Substances 0.000 description 1
• 239000008055 phosphate buffer solution Substances 0.000 description 1
• 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
• HMFAQQIORZDPJG-UHFFFAOYSA-N phosphono 2-chloroacetate Chemical compound OP(O)(=O)OC(=O)CCl HMFAQQIORZDPJG-UHFFFAOYSA-N 0.000 description 1
• 150000008300 phosphoramidites Chemical class 0.000 description 1
• LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 description 1
• 125000005642 phosphothioate group Chemical group 0.000 description 1
• 239000003757 phosphotransferase inhibitor Substances 0.000 description 1
• 230000004962 physiological condition Effects 0.000 description 1
• 239000000049 pigment Substances 0.000 description 1
• 239000006187 pill Substances 0.000 description 1
• 210000002381 plasma Anatomy 0.000 description 1
• 239000004014 plasticizer Substances 0.000 description 1
• 210000001778 pluripotent stem cell Anatomy 0.000 description 1
• 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
• 208000003580 polydactyly Diseases 0.000 description 1
• 229940068886 polyethylene glycol 300 Drugs 0.000 description 1
• 229920000642 polymer Polymers 0.000 description 1
• 239000001816 polyoxyethylene sorbitan tristearate Substances 0.000 description 1
• 235000010988 polyoxyethylene sorbitan tristearate Nutrition 0.000 description 1
• 229920001282 polysaccharide Polymers 0.000 description 1
• 239000005017 polysaccharide Substances 0.000 description 1
• 150000004804 polysaccharides Chemical class 0.000 description 1
• 229940099511 polysorbate 65 Drugs 0.000 description 1
• 208000007232 portal hypertension Diseases 0.000 description 1
• 229920001592 potato starch Polymers 0.000 description 1
• 239000003755 preservative agent Substances 0.000 description 1
• 230000002335 preservative effect Effects 0.000 description 1
• 201000008312 primary pulmonary hypertension Diseases 0.000 description 1
• 230000037452 priming Effects 0.000 description 1
• 238000012545 processing Methods 0.000 description 1
• 230000005522 programmed cell death Effects 0.000 description 1
• 230000002250 progressing effect Effects 0.000 description 1
• 230000009465 prokaryotic expression Effects 0.000 description 1
• 230000002062 proliferating effect Effects 0.000 description 1
• 230000031877 prophase Effects 0.000 description 1
• 235000019260 propionic acid Nutrition 0.000 description 1
• 230000026447 protein localization Effects 0.000 description 1
• 230000002797 proteolythic effect Effects 0.000 description 1
• 239000003586 protic polar solvent Substances 0.000 description 1
• 210000001938 protoplast Anatomy 0.000 description 1
• UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
• 229960003581 pyridoxal Drugs 0.000 description 1
• 235000008164 pyridoxal Nutrition 0.000 description 1
• 239000011674 pyridoxal Substances 0.000 description 1
• 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 1
• 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 1
• 229960001327 pyridoxal phosphate Drugs 0.000 description 1
• 125000004076 pyridyl group Chemical class 0.000 description 1
• 125000000714 pyrimidinyl group Chemical group 0.000 description 1
• 238000004445 quantitative analysis Methods 0.000 description 1
• IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
• 230000009257 reactivity Effects 0.000 description 1
• 210000003370 receptor cell Anatomy 0.000 description 1
• 238000003259 recombinant expression Methods 0.000 description 1
• NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
• 230000008929 regeneration Effects 0.000 description 1
• 238000011069 regeneration method Methods 0.000 description 1
• 230000009703 regulation of cell differentiation Effects 0.000 description 1
• 230000031539 regulation of cell division Effects 0.000 description 1
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
• 230000009711 regulatory function Effects 0.000 description 1
• 230000003362 replicative effect Effects 0.000 description 1
• 230000000717 retained effect Effects 0.000 description 1
• 230000002441 reversible effect Effects 0.000 description 1
• 238000012552 review Methods 0.000 description 1
• 108091092562 ribozyme Proteins 0.000 description 1
• 229940100486 rice starch Drugs 0.000 description 1
• XMVJITFPVVRMHC-UHFFFAOYSA-N roxarsone Chemical group OC1=CC=C([As](O)(O)=O)C=C1[N+]([O-])=O XMVJITFPVVRMHC-UHFFFAOYSA-N 0.000 description 1
• 230000007017 scission Effects 0.000 description 1
• 230000003248 secreting effect Effects 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 238000005204 segregation Methods 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 150000003346 selenoethers Chemical class 0.000 description 1
• 230000009758 senescence Effects 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 238000002864 sequence alignment Methods 0.000 description 1
• 239000008159 sesame oil Substances 0.000 description 1
• 235000011803 sesame oil Nutrition 0.000 description 1
• 108091006024 signal transducing proteins Proteins 0.000 description 1
• 102000034285 signal transducing proteins Human genes 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 235000020183 skimmed milk Nutrition 0.000 description 1
• 239000002002 slurry Substances 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• 239000011734 sodium Substances 0.000 description 1
• 229910052708 sodium Inorganic materials 0.000 description 1
• 235000010413 sodium alginate Nutrition 0.000 description 1
• 239000000661 sodium alginate Substances 0.000 description 1
• 229940005550 sodium alginate Drugs 0.000 description 1
• 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
• 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
• IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
• 239000007901 soft capsule Substances 0.000 description 1
• 239000002904 solvent Substances 0.000 description 1
• 230000003595 spectral effect Effects 0.000 description 1
• 210000004988 splenocyte Anatomy 0.000 description 1
• 238000012453 sprague-dawley rat model Methods 0.000 description 1
• 239000007921 spray Substances 0.000 description 1
• 238000005507 spraying Methods 0.000 description 1
• 239000007858 starting material Substances 0.000 description 1
• 230000037351 starvation Effects 0.000 description 1
• 238000003756 stirring Methods 0.000 description 1
• 210000001768 subcellular fraction Anatomy 0.000 description 1
• 230000004960 subcellular localization Effects 0.000 description 1
• 125000001424 substituent group Chemical group 0.000 description 1
• 239000005720 sucrose Substances 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 239000000829 suppository Substances 0.000 description 1
• 239000002511 suppository base Substances 0.000 description 1
• 230000004083 survival effect Effects 0.000 description 1
• 239000000375 suspending agent Substances 0.000 description 1
• 230000002194 synthesizing effect Effects 0.000 description 1
• 229920002994 synthetic fiber Polymers 0.000 description 1
• 239000006188 syrup Substances 0.000 description 1
• 235000020357 syrup Nutrition 0.000 description 1
• 238000007910 systemic administration Methods 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 239000011975 tartaric acid Substances 0.000 description 1
• 235000002906 tartaric acid Nutrition 0.000 description 1
• 208000009056 telangiectasis Diseases 0.000 description 1
• 208000001608 teratocarcinoma Diseases 0.000 description 1
• 231100001274 therapeutic index Toxicity 0.000 description 1
• 238000002560 therapeutic procedure Methods 0.000 description 1
• 230000008719 thickening Effects 0.000 description 1
• 229940113082 thymine Drugs 0.000 description 1
• 239000004408 titanium dioxide Substances 0.000 description 1
• 235000010487 tragacanth Nutrition 0.000 description 1
• 239000000196 tragacanth Substances 0.000 description 1
• 229940116362 tragacanth Drugs 0.000 description 1
• 230000005758 transcription activity Effects 0.000 description 1
• 238000010361 transduction Methods 0.000 description 1
• 230000026683 transduction Effects 0.000 description 1
• 238000001890 transfection Methods 0.000 description 1
• 238000003151 transfection method Methods 0.000 description 1
• 238000006276 transfer reaction Methods 0.000 description 1
• 238000012256 transgenic experiment Methods 0.000 description 1
• 238000011830 transgenic mouse model Methods 0.000 description 1
• 230000001052 transient effect Effects 0.000 description 1
• 238000003146 transient transfection Methods 0.000 description 1
• 230000005945 translocation Effects 0.000 description 1
• 150000003626 triacylglycerols Chemical class 0.000 description 1
• YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
• CYRMSUTZVYGINF-UHFFFAOYSA-N trichlorofluoromethane Chemical compound FC(Cl)(Cl)Cl CYRMSUTZVYGINF-UHFFFAOYSA-N 0.000 description 1
• 229940029284 trichlorofluoromethane Drugs 0.000 description 1
• 150000005691 triesters Chemical class 0.000 description 1
• 239000001226 triphosphate Substances 0.000 description 1
• 235000011178 triphosphate Nutrition 0.000 description 1
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
• PZYFJWVGRGEWGO-UHFFFAOYSA-N trisodium;hydrogen peroxide;trioxido(oxo)vanadium Chemical compound [Na+].[Na+].[Na+].OO.OO.OO.[O-][V]([O-])([O-])=O PZYFJWVGRGEWGO-UHFFFAOYSA-N 0.000 description 1
• 230000004565 tumor cell growth Effects 0.000 description 1
• 230000004614 tumor growth Effects 0.000 description 1
• 239000000225 tumor suppressor protein Substances 0.000 description 1
• 238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
• 238000001323 two-dimensional chromatography Methods 0.000 description 1
• 108010002164 tyrosine receptor Proteins 0.000 description 1
• 229940035936 ubiquinone Drugs 0.000 description 1
• 238000011144 upstream manufacturing Methods 0.000 description 1
• 210000002700 urine Anatomy 0.000 description 1
• 238000001291 vacuum drying Methods 0.000 description 1
• 208000019553 vascular disease Diseases 0.000 description 1
• 230000009385 viral infection Effects 0.000 description 1
• 230000003612 virological effect Effects 0.000 description 1
• 230000003313 weakening effect Effects 0.000 description 1
• 238000001238 wet grinding Methods 0.000 description 1
• 229940100445 wheat starch Drugs 0.000 description 1
• 230000029663 wound healing Effects 0.000 description 1
• 210000005253 yeast cell Anatomy 0.000 description 1
• 235000021249 α-casein Nutrition 0.000 description 1