JP2002507881A - 樹枝状細胞前駆体のインビトロ増殖の方法およびその免疫原製造への使用 - Google Patents
樹枝状細胞前駆体のインビトロ増殖の方法およびその免疫原製造への使用Info
- Publication number
- JP2002507881A JP2002507881A JP51773893A JP51773893A JP2002507881A JP 2002507881 A JP2002507881 A JP 2002507881A JP 51773893 A JP51773893 A JP 51773893A JP 51773893 A JP51773893 A JP 51773893A JP 2002507881 A JP2002507881 A JP 2002507881A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- antigen
- dendritic
- dendritic cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004443 dendritic cell Anatomy 0.000 title claims abstract description 563
- 239000002243 precursor Substances 0.000 title claims abstract description 140
- 238000000034 method Methods 0.000 title claims description 176
- 238000000338 in vitro Methods 0.000 title claims description 25
- 238000004519 manufacturing process Methods 0.000 title abstract description 20
- 230000002163 immunogen Effects 0.000 title description 22
- 239000000427 antigen Substances 0.000 claims abstract description 291
- 108091007433 antigens Proteins 0.000 claims abstract description 287
- 102000036639 antigens Human genes 0.000 claims abstract description 287
- 210000001744 T-lymphocyte Anatomy 0.000 claims abstract description 74
- 238000011282 treatment Methods 0.000 claims abstract description 19
- 229960005486 vaccine Drugs 0.000 claims abstract description 12
- 208000023275 Autoimmune disease Diseases 0.000 claims abstract description 9
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 claims abstract description 5
- 201000006417 multiple sclerosis Diseases 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 533
- 230000001464 adherent effect Effects 0.000 claims description 81
- 210000001185 bone marrow Anatomy 0.000 claims description 81
- 239000008280 blood Substances 0.000 claims description 73
- 210000004369 blood Anatomy 0.000 claims description 72
- 210000001519 tissue Anatomy 0.000 claims description 61
- 239000002609 medium Substances 0.000 claims description 52
- 239000000203 mixture Substances 0.000 claims description 30
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 28
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 27
- 238000012258 culturing Methods 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 22
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 claims description 20
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 claims description 20
- 230000001965 increasing effect Effects 0.000 claims description 20
- 230000008569 process Effects 0.000 claims description 19
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 17
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims description 16
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 claims description 16
- 230000000295 complement effect Effects 0.000 claims description 16
- 239000012634 fragment Substances 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 15
- 238000004113 cell culture Methods 0.000 claims description 13
- 102000000646 Interleukin-3 Human genes 0.000 claims description 12
- 108010002386 Interleukin-3 Proteins 0.000 claims description 12
- 210000004698 lymphocyte Anatomy 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims description 11
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims description 11
- 239000012894 fetal calf serum Substances 0.000 claims description 11
- 210000002966 serum Anatomy 0.000 claims description 9
- 230000003053 immunization Effects 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 claims description 6
- 201000008827 tuberculosis Diseases 0.000 claims description 6
- 102000000589 Interleukin-1 Human genes 0.000 claims description 5
- 108010002352 Interleukin-1 Proteins 0.000 claims description 5
- 230000011132 hemopoiesis Effects 0.000 claims description 5
- 210000005087 mononuclear cell Anatomy 0.000 claims description 5
- 230000003612 virological effect Effects 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 208000031295 Animal disease Diseases 0.000 claims description 3
- 206010012438 Dermatitis atopic Diseases 0.000 claims description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 3
- 201000008937 atopic dermatitis Diseases 0.000 claims description 3
- 101710098119 Chaperonin GroEL 2 Proteins 0.000 claims description 2
- 206010028417 myasthenia gravis Diseases 0.000 claims description 2
- 241000187479 Mycobacterium tuberculosis Species 0.000 claims 4
- 230000003394 haemopoietic effect Effects 0.000 claims 1
- 239000013603 viral vector Substances 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 abstract description 38
- 230000012010 growth Effects 0.000 abstract description 26
- 230000001419 dependent effect Effects 0.000 abstract description 21
- 201000010099 disease Diseases 0.000 abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- 210000003714 granulocyte Anatomy 0.000 description 41
- 241000699666 Mus <mouse, genus> Species 0.000 description 36
- 210000002540 macrophage Anatomy 0.000 description 34
- 108091054438 MHC class II family Proteins 0.000 description 31
- 239000002245 particle Substances 0.000 description 30
- 102000043131 MHC class II family Human genes 0.000 description 29
- 108090000623 proteins and genes Proteins 0.000 description 29
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 28
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 27
- 230000028993 immune response Effects 0.000 description 27
- 102000004169 proteins and genes Human genes 0.000 description 24
- 210000000265 leukocyte Anatomy 0.000 description 23
- 108090000765 processed proteins & peptides Proteins 0.000 description 23
- 235000018102 proteins Nutrition 0.000 description 23
- 239000000047 product Substances 0.000 description 21
- 239000008187 granular material Substances 0.000 description 20
- 210000000952 spleen Anatomy 0.000 description 19
- 241000699670 Mus sp. Species 0.000 description 17
- 230000004044 response Effects 0.000 description 17
- 230000004936 stimulating effect Effects 0.000 description 16
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 15
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 15
- 238000002372 labelling Methods 0.000 description 15
- 210000002751 lymph Anatomy 0.000 description 15
- 238000010186 staining Methods 0.000 description 15
- 102100034540 Adenomatous polyposis coli protein Human genes 0.000 description 14
- 101000924577 Homo sapiens Adenomatous polyposis coli protein Proteins 0.000 description 14
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 14
- 230000006870 function Effects 0.000 description 14
- 230000000242 pagocytic effect Effects 0.000 description 14
- 102000004196 processed proteins & peptides Human genes 0.000 description 14
- 102000004127 Cytokines Human genes 0.000 description 13
- 108090000695 Cytokines Proteins 0.000 description 13
- 229940098773 bovine serum albumin Drugs 0.000 description 13
- 206010057249 Phagocytosis Diseases 0.000 description 12
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 12
- 229910052799 carbon Inorganic materials 0.000 description 12
- 210000003743 erythrocyte Anatomy 0.000 description 12
- 239000004816 latex Substances 0.000 description 12
- 229920000126 latex Polymers 0.000 description 12
- 230000035800 maturation Effects 0.000 description 12
- 230000008782 phagocytosis Effects 0.000 description 12
- 239000002356 single layer Substances 0.000 description 12
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 11
- 244000005700 microbiome Species 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 10
- 210000000612 antigen-presenting cell Anatomy 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 9
- 230000030741 antigen processing and presentation Effects 0.000 description 9
- 230000003834 intracellular effect Effects 0.000 description 9
- 210000001165 lymph node Anatomy 0.000 description 9
- 102000013415 peroxidase activity proteins Human genes 0.000 description 9
- 108040007629 peroxidase activity proteins Proteins 0.000 description 9
- 230000003389 potentiating effect Effects 0.000 description 9
- 230000035755 proliferation Effects 0.000 description 9
- 210000000130 stem cell Anatomy 0.000 description 9
- 239000000725 suspension Substances 0.000 description 9
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 8
- 239000002671 adjuvant Substances 0.000 description 8
- 210000001787 dendrite Anatomy 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 230000000638 stimulation Effects 0.000 description 8
- 229940104230 thymidine Drugs 0.000 description 8
- 102000043129 MHC class I family Human genes 0.000 description 7
- 108091054437 MHC class I family Proteins 0.000 description 7
- 101150052863 THY1 gene Proteins 0.000 description 7
- 230000000890 antigenic effect Effects 0.000 description 7
- 210000000601 blood cell Anatomy 0.000 description 7
- 210000002798 bone marrow cell Anatomy 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000002380 cytological effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 210000004700 fetal blood Anatomy 0.000 description 7
- 239000012737 fresh medium Substances 0.000 description 7
- 238000001727 in vivo Methods 0.000 description 7
- 208000015181 infectious disease Diseases 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 210000001616 monocyte Anatomy 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 101001046686 Homo sapiens Integrin alpha-M Proteins 0.000 description 6
- 102100022338 Integrin alpha-M Human genes 0.000 description 6
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 210000004907 gland Anatomy 0.000 description 6
- 230000003308 immunostimulating effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 6
- 230000037361 pathway Effects 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 102100032912 CD44 antigen Human genes 0.000 description 5
- 229930182566 Gentamicin Natural products 0.000 description 5
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 235000019270 ammonium chloride Nutrition 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000000376 autoradiography Methods 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 229960002518 gentamicin Drugs 0.000 description 5
- 230000036039 immunity Effects 0.000 description 5
- 210000003563 lymphoid tissue Anatomy 0.000 description 5
- 238000004091 panning Methods 0.000 description 5
- 239000004033 plastic Substances 0.000 description 5
- 210000003491 skin Anatomy 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 210000003934 vacuole Anatomy 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 5
- 241000699800 Cricetinae Species 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 238000002738 Giemsa staining Methods 0.000 description 4
- 102000009490 IgG Receptors Human genes 0.000 description 4
- 108010073807 IgG Receptors Proteins 0.000 description 4
- 102000004889 Interleukin-6 Human genes 0.000 description 4
- 108090001005 Interleukin-6 Proteins 0.000 description 4
- BAQCROVBDNBEEB-UBYUBLNFSA-N Metrizamide Chemical compound CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C(=O)N[C@@H]2[C@H]([C@H](O)[C@@H](CO)OC2O)O)=C1I BAQCROVBDNBEEB-UBYUBLNFSA-N 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- -1 T NF-α Proteins 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000000735 allogeneic effect Effects 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 238000009640 blood culture Methods 0.000 description 4
- 210000000988 bone and bone Anatomy 0.000 description 4
- 238000001493 electron microscopy Methods 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 210000001821 langerhans cell Anatomy 0.000 description 4
- 210000002414 leg Anatomy 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 229960000554 metrizamide Drugs 0.000 description 4
- 210000001539 phagocyte Anatomy 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000004043 responsiveness Effects 0.000 description 4
- 230000002000 scavenging effect Effects 0.000 description 4
- 210000002536 stromal cell Anatomy 0.000 description 4
- 239000013589 supplement Substances 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000283707 Capra Species 0.000 description 3
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 3
- 102000018713 Histocompatibility Antigens Class II Human genes 0.000 description 3
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 3
- 206010025323 Lymphomas Diseases 0.000 description 3
- 101000746372 Mus musculus Granulocyte-macrophage colony-stimulating factor Proteins 0.000 description 3
- 241000699667 Mus spretus Species 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 230000018199 S phase Effects 0.000 description 3
- 230000005867 T cell response Effects 0.000 description 3
- 230000003698 anagen phase Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000002121 endocytic effect Effects 0.000 description 3
- 230000001605 fetal effect Effects 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 230000009851 immunogenic response Effects 0.000 description 3
- 230000005847 immunogenicity Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 210000003712 lysosome Anatomy 0.000 description 3
- 230000001868 lysosomic effect Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000004379 membrane Anatomy 0.000 description 3
- 230000015654 memory Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 210000000440 neutrophil Anatomy 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 230000000750 progressive effect Effects 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 210000004989 spleen cell Anatomy 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 210000000689 upper leg Anatomy 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 210000001239 CD8-positive, alpha-beta cytotoxic T lymphocyte Anatomy 0.000 description 2
- 108010051152 Carboxylesterase Proteins 0.000 description 2
- 102000013392 Carboxylesterase Human genes 0.000 description 2
- 108090000371 Esterases Proteins 0.000 description 2
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 102100036242 HLA class II histocompatibility antigen, DQ alpha 2 chain Human genes 0.000 description 2
- 102000006354 HLA-DR Antigens Human genes 0.000 description 2
- 108010058597 HLA-DR Antigens Proteins 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 101000930801 Homo sapiens HLA class II histocompatibility antigen, DQ alpha 2 chain Proteins 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 102100022297 Integrin alpha-X Human genes 0.000 description 2
- 102000004125 Interleukin-1alpha Human genes 0.000 description 2
- 108010082786 Interleukin-1alpha Proteins 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- 208000012868 Overgrowth Diseases 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- 241000282806 Rhinoceros Species 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000005784 autoimmunity Effects 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 230000002902 bimodal effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000011712 cell development Effects 0.000 description 2
- 230000003915 cell function Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 230000012202 endocytosis Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000012921 fluorescence analysis Methods 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 210000002443 helper t lymphocyte Anatomy 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 238000009169 immunotherapy Methods 0.000 description 2
- CGIGDMFJXJATDK-UHFFFAOYSA-N indomethacin Chemical compound CC1=C(CC(O)=O)C2=CC(OC)=CC=C2N1C(=O)C1=CC=C(Cl)C=C1 CGIGDMFJXJATDK-UHFFFAOYSA-N 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 229940100601 interleukin-6 Drugs 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000644 isotonic solution Substances 0.000 description 2
- 208000032839 leukemia Diseases 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 239000013618 particulate matter Substances 0.000 description 2
- 210000003024 peritoneal macrophage Anatomy 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000009696 proliferative response Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000001235 sensitizing effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 210000004988 splenocyte Anatomy 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000003981 vehicle Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 238000011740 C57BL/6 mouse Methods 0.000 description 1
- 241000272194 Ciconiiformes Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 1
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 1
- 108010060123 Conjugate Vaccines Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 206010012442 Dermatitis contact Diseases 0.000 description 1
- 241001061260 Emmelichthys struhsakeri Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 102000009109 Fc receptors Human genes 0.000 description 1
- 108010087819 Fc receptors Proteins 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- 239000012981 Hank's balanced salt solution Substances 0.000 description 1
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 1
- 102100022623 Hepatocyte growth factor receptor Human genes 0.000 description 1
- 101710184069 Hepatocyte growth factor receptor Proteins 0.000 description 1
- 102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 1
- 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 1
- 108010027412 Histocompatibility Antigens Class II Proteins 0.000 description 1
- 101000935040 Homo sapiens Integrin beta-2 Proteins 0.000 description 1
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 102100025390 Integrin beta-2 Human genes 0.000 description 1
- 108010032774 Interleukin-2 Receptor alpha Subunit Proteins 0.000 description 1
- 102000007351 Interleukin-2 Receptor alpha Subunit Human genes 0.000 description 1
- 108010038453 Interleukin-2 Receptors Proteins 0.000 description 1
- 102000010789 Interleukin-2 Receptors Human genes 0.000 description 1
- 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 208000030729 Juvenile myasthenia gravis Diseases 0.000 description 1
- 235000019766 L-Lysine Nutrition 0.000 description 1
- 235000001008 Leptadenia hastata Nutrition 0.000 description 1
- 244000074209 Leptadenia hastata Species 0.000 description 1
- 108010013709 Leukocyte Common Antigens Proteins 0.000 description 1
- 102000017095 Leukocyte Common Antigens Human genes 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 108010030317 Macrophage-1 Antigen Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000003979 Mineralocorticoid Receptors Human genes 0.000 description 1
- 108090000375 Mineralocorticoid Receptors Proteins 0.000 description 1
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 1
- 241000234295 Musa Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 208000033776 Myeloid Acute Leukemia Diseases 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 101100289792 Squirrel monkey polyomavirus large T gene Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 230000006044 T cell activation Effects 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 1
- GPKUGWDQUVWHIC-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine tetrahydrochloride Chemical compound Cl.Cl.Cl.Cl.NNC1=CC=C(C=C1)C1=CC=C(NN)C=C1 GPKUGWDQUVWHIC-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000012711 adhesive precursor Substances 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 229940037003 alum Drugs 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003460 anti-nuclear Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 description 1
- 230000006472 autoimmune response Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000005208 blood dendritic cell Anatomy 0.000 description 1
- 229960000182 blood factors Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001721 carbon Chemical class 0.000 description 1
- 238000012754 cardiac puncture Methods 0.000 description 1
- 210000001715 carotid artery Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 238000003320 cell separation method Methods 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000017455 cell-cell adhesion Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 229940031670 conjugate vaccine Drugs 0.000 description 1
- 238000011441 consolidation chemotherapy Methods 0.000 description 1
- 208000010247 contact dermatitis Diseases 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000012303 cytoplasmic staining Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 210000004544 dc2 Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000779 depleting effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000012645 endogenous antigen Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000001973 epigenetic effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 210000003811 finger Anatomy 0.000 description 1
- 238000005188 flotation Methods 0.000 description 1
- 108010081400 fluorescein isothiocyante avidin Proteins 0.000 description 1
- 210000002683 foot Anatomy 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 210000004565 granule cell Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 208000013403 hyperactivity Diseases 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000006054 immunological memory Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000000937 inactivator Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229960000905 indomethacin Drugs 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229940076264 interleukin-3 Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 210000005210 lymphoid organ Anatomy 0.000 description 1
- 230000002132 lysosomal effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000005868 ontogenesis Effects 0.000 description 1
- 230000001936 parietal effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000000680 phagosome Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 210000004986 primary T-cell Anatomy 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 210000001948 pro-b lymphocyte Anatomy 0.000 description 1
- 230000007425 progressive decline Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 210000001243 pseudopodia Anatomy 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000003134 recirculating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 210000003660 reticulum Anatomy 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- YEENEYXBHNNNGV-XEHWZWQGSA-M sodium;3-acetamido-5-[acetyl(methyl)amino]-2,4,6-triiodobenzoate;(2r,3r,4s,5s,6r)-2-[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound [Na+].CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I.O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 YEENEYXBHNNNGV-XEHWZWQGSA-M 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
- 210000002303 tibia Anatomy 0.000 description 1
- 210000003371 toe Anatomy 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0639—Dendritic cells, e.g. Langherhans cells in the epidermis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
- A61K39/0008—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4615—Dendritic cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
- A61K39/4621—Cellular immunotherapy characterized by the effect or the function of the cells immunosuppressive or immunotolerising
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
- A61K39/4622—Antigen presenting cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/46433—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4648—Bacterial antigens
- A61K39/464819—Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/122—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells for inducing tolerance or supression of immune responses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/515—Animal cells
- A61K2039/5154—Antigen presenting cells [APCs], e.g. dendritic cells or macrophages
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/25—Tumour necrosing factors [TNF]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Rheumatology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.(a)樹枝状細胞前駆体を含む組織源を準備し、 (b)(a)からの組織源を処理して樹枝状細胞前駆体の比率を増加させ、 インビトロ培養に適した細胞集団を得、 (c)GM―CSFを含む培養培地中の基体上で、この細胞源を培養して、 非付着性細胞および細胞集団を得、 (d)この非付着性細胞および細胞集団を継代培養して増殖する樹枝状細胞 前駆体を含む細胞凝集体を製造し、 (e)この細胞凝集体を一回以上順次継代培養して樹枝状細胞前駆体の比率 を増加させることを特徴とする増殖している細胞培養物から樹枝状細胞の集団 を製造する方法。 2.上記組織源が血液または骨髄であり、GM―CSFが約1−1000U/ml の濃度で上記培地中に存在する請求項1の方法。 3.上記組織源が骨髄である時、処理工程(b)が、更に、補体を含む媒体中で 樹枝状細胞前駆体上に存在しない抗原に対する特異抗体と、骨髄を接触するこ とによって、樹枝状細胞前駆体上で発現されない抗原を発現する細胞を殺す工 程を含む請求項2の方法。 4.上記組織源が骨髄で、上記抗体が、Ia抗原、T細胞上に存在する抗原およ び成熟樹枝状細胞上に存在する抗原からなる群から選ばれる一個以上の抗原に 対するものである請求項3の方法。 5.上記骨髄が、約500−1000U/mlの濃度のrGM―CSFで培養され る請求項4の方法。 6.Ia−陰性骨髄非リンパ球が約5×105細胞/cm2の濃度で培養される請求 項5の方法。 7.抗−Ia抗原抗体と抗−T細胞、B細胞および単核細胞抗体が、GK1.5抗 −CD4、Ho2.2抗−CD8、B21−2抗−Ia、およびRA3−3A1 /6.1抗−B220/CD45Rからなる群から選ばれる請求項6の方法。 8.工程(e)の細胞凝集体が1ないし5回連続して継代培養されている請求項 3の方法。 9.上記細胞凝集体が2ないし3回連続して継代培養されている請求項8の方法 。 10.上記細胞凝集体が2回連続して継代培養されている請求項9の方法。 11.工程(c)の非付着性細胞および細胞集団を約0.3ないし1日の後に、継代培 養し、上記細胞凝集体を全て3ないし30日連続して継代培養する請求項3の 方法。 12.上記細胞凝集体を全て10ないし20日連続して継代培養する請求項11の 方法。 13.上記細胞凝集体を全て20日連続して継代培養する請求項12の方法。 14.上記細胞源が血液または骨髄で、工程(c)の非付着性細胞および細胞集団 を約0.3ないし1日の後に、継代培養し、上記細胞凝集体を全て3ないし30 日連続して継代培養する請求項3の方法。 15.工程(c)の非付着性細胞および細胞集団を約半日後に、継代培養し、上記 細胞凝集体を20日後に二度連続して継代培養する請求項14の方法。 16.上記培養培地が、RPMI1640、DMEMおよびα−MEMからなる群 から選ばれるものであり、かつ上記培養培地が血清で補足されている請求項3 の方法。 17.牛胎児の血清が上記培養培地に約1〜15%の量で存在する請求項16の方 法。 18.牛胎児の血清が上記培養培地に約10%の量で存在する請求項17の方法。 19.上記細胞源が血液で、培地中のGM―CSF濃度が約30−100U/mlで ある請求項14の方法。 20.上記細胞源が骨髄で、培地中のGM―CSF濃度が約500−1000U/ mlである請求項14の方法。 21.(a)樹枝状細胞前駆体を含む組織源を準備し、 (b)(a)からの組織源を処理して樹枝状細胞前駆体の比率を増加させ、 インビトロ培養に適した細胞集団を得、 (c)GM―CSFを含む培養培地中の基体上で、この細胞源を培養して、 非付着性細胞および細胞集団を得、 (d)この非付着性細胞および細胞集団を継代培養して増殖する樹枝状細胞 前駆体を含む細胞凝集体を製造し、 (e)この細胞凝集体を一回以上順次継代培養して、樹枝状細胞前駆体の比 率を増加させ、 (f)この樹枝状細胞前駆体を、それらを成熟樹枝状細胞に熟成させるに充 分な時間、培養し続けることを特徴とする増殖している細胞培養物から成熟樹 枝状細胞の集団を製造する方法。 22.上記組織源が血液または骨髄であり、GM―CSFが約1−1000U/ml の濃度で上記培地中に存在する請求項21の方法。 23.上記組織源が骨髄である時、全処理段階で、補体を含む媒体中で樹枝状細胞 前駆体上に存在しない抗原に対する特異抗体と、骨髄を接触することによって 、樹枝状細胞前駆体上で発現されない抗原を発現する細胞を殺す工程を含む請 求項21の方法。 24.上記組織源が骨髄で、上記抗体が、Ia抗原、T細胞上に存在する抗原およ び成熟樹枝状細胞上に存在する抗原からなる群から選ばれる一個以上の抗原に 対するものである請求項23の方法。 25.上記骨髄が、約500−1000U/mlの濃度のrGM―CSFで培養され る請求項24の方法。 26.Ia−陰性骨髄非リンパ球が細胞濃度約5×105/cm2で培養される請求項 25の方法。 27.抗−Ia抗原抗体と抗−T細胞、B細胞および単核細胞抗体が、GK1.5抗− CD4、Ho2.2抗−CD8、B21−2抗−Ia、およびRA3−3A1/6 .1抗−B220/45Rからなる群から選ばれる請求項25の方法。 28.工程(e)の細胞凝集体が1ないし5回連続して継代培養されている請求項 21の方法。 29.上記細胞凝集体が2ないし3回連続して継代培養されている請求項28の方 法。 30.上記細胞凝集体が2回連続して継代培養されている請求項29の方法。 31.工程(c)の非付着性細胞および細胞集団を、約0.3ないし1日の後に、継 代培養し、上記細胞凝集体を全て3ないし30日連続して継代培養する請求項 21の方法。 32.上記細胞凝集体を全て10ないし20日連続して継代培養する請求項31の 方法。 33.上記細胞凝集体を全て20日連続して継代培養する請求項32の方法。 34.上記細胞凝集体を1ないし5回連続して継代培養する請求項31の方法。 35.工程(c)の非付着性細胞および細胞集団を約半日後に、継代培養し、上記 細胞凝集体を20日後に二度連続して継代培養する請求項29の方法。 36.上記培養培地が、RPMI1640、DMEMおよびα−MEMからなる群 から選ばれるものであり、かつ上記培養培地が血清で補足されている請求項2 1の方法。 37.牛胎児の血清が上記培養培地に約1〜15%の量で存在する請求項36の方 法。 38.牛胎児の血清が上記培養培地に約10%の量で存在する請求項37の方法。 39.上記細胞源が血液で、培地中にGM―CSFが約30−100U/mlの濃度 で存在する請求項21の方法。 40.上記細胞源が骨髄で、培地中にGM―CSFが約500−1000U/mlの 濃度で存在する請求項21の方法。 41.抗原を請求項21、39または40のいずれかの方法で得た樹枝状細胞の集 団に曝し、抗原−活性化した樹枝状細胞を製造し、次いで、この抗原−活性化 した樹枝状細胞をホストに接種することを特徴とするホストに抗原を付与する 方法。 42.ホストが人間である請求項41の方法。 43.請求項21、39または40のいずれかの方法で調製された樹枝状細胞を含 む組成物。 44.請求項21で調製された樹枝状細胞が抗原でパルスされ、樹枝状細胞が上記 抗原を処理し、この樹枝状細胞で発現される変性された抗原を製造することを 特徴とする抗原−活性化した樹枝状細胞を含む組成物。 45.変性さるべき基体を、請求項21、39または40のいずれかの方法で得た 樹枝状細胞の集団に曝し、この基体を樹技状細胞で変性し、変性した抗原を製 造することを特徴とする樹枝状細胞変性した抗原を含む組成物。 46.請求項44の組成物を含むワクチンを投与することを特徴とする人間または 動物の病気に対して免疫にする方法。 47.請求項44の組成物を含むワクチン。 48.請求項45の組成物を含むワクチンを投与することを特徴とする人間または 動物の病気に対して免疫にする方法。 49.請求項45の組成物を含むワクチン。 50.請求項44の組成物の治療効果のある量を治療の必要な人に投与するもので 、変性さるべき抗原が自己−蛋白質であることを特徴とする自己免疫疾患を治 療する方法。 51.請求項45の組成物の治療効果のある量を治療の必要な人に投与するもので 、変性さるべき抗原が自己−蛋白質であることを特徴とする自己免疫疾患を治 療する方法。 52.自己免疫疾患が、多発硬化症、重症筋無力症、アトピー性皮膚炎および若年 糖尿病からなる群から選ばれる請求項50の方法。 53.自己免疫疾患が、多発硬化症および若年糖尿病からなる群から選ばれる請求 項51の方法。 54.請求項1の方法で調製される樹枝状細胞前駆体。 55.組織源が血液または骨髄である請求項46による樹枝状細胞前駆体。 56.組織源が血液である請求項55による樹枝状細胞前駆体。 57.組織源が骨髄である請求項47による樹枝状細胞前駆体。 58.組織源が人間の血液で、培地中にGM―CSFが約400−800U/mlの 濃度で存在する請求項2の方法。 59.TNF−α、G―CSF、IL―1およびIL―3からなる群から選ばれる 一個以上のファクターが、培養培地中に存在する請求項58の方法。 60.組織源が人間の血液で、培地中にGM―CSFが約400−800U/mlの 濃度で存在する請求項14の方法。 61.TNF−α、G―CSF、IL―1およびIL―3からなる群から選ばれる 一個以上のファクターが、培養培地中に存在する請求項60の方法。 62.組織源が人間の血液で、培地中にGM―CSFが約400−800U/mlの 濃度で存在する請求項22の方法。 63.TNF−α、G―CSF、IL―1およびIL―3からなる群から選ばれる 一個以上のファクターが、培養培地中に存在する請求項62の方法。 64.上記細胞が人間の血液から得られ、約400−800U/mlの濃度のGM− CSFの存在下で培養される請求項56による樹枝状細胞前駆体。 65.抗原から抗原フラグメントを調製する方法で、樹枝状細胞および樹枝状細胞 前駆体からなる群から選ばれる細胞と抗原を接触させ、該細胞が抗原をフラグ メントに加工し、細胞表面に抗原フラグメントが存在するに充分な時間、該細 胞と抗原を培養することを特徴とする抗原から抗原フラグメントを調製する方 法。 66.樹枝状細胞または樹枝状細胞前駆体が血液または骨髄から得られたものであ る請求項65の方法。 67.樹枝状細胞または樹枝状細胞前駆体が血液から得られたものである請求項6 6の方法。 68.樹枝状細胞または樹枝状細胞前駆体が人間の血液から得られ、約400−8 00U/mlの濃度のGM―CSFの存在下で培養されたものである請求項67 の方法。 69.抗原が樹枝状細胞前駆体により食作用を受ける請求項65の方法。 70.抗原がミコバクテリア抗原、バクテリア抗原およびウイルス抗原からなる群 から選ばれる請求項69の方法。 71.抗原がミコバクテリア結核菌である請求項70の方法。 72.ミコバクテリア結核菌がBCGである請求項71の方法。 73.請求項65の方法で調製された抗原フラグメント。 74.抗原が食作用を受ける請求項73の抗原フラグメント。 75.抗原がミコバクテリア抗原、バクテリア抗原またはウイルス抗原からなる群 から選ばれる請求項74の抗原フラグメント。 76.抗原がミコバクテリア結核菌抗原である請求項75の抗原フラグメント。 77.ミコバクテリア結核菌がBCGである請求項76の抗原フラグメント。 78.抗原が樹技状細胞前駆体により食作用を受けるウイルスベクターである請求 項75の抗原フラグメント。 79.請求項77の抗原フラグメントの治療効果ある量を投与することを特徴とす る結核の治療法。 80.組織源が、個人から組織源を取り出す前に造血を刺激するような物質で前処 理された個人から得られたものである請求項1の方法。 81.造血物質がGM―CSFおよびG―CSFからなる群から選ばれる請求項8 0の方法。
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US86161292A | 1992-04-01 | 1992-04-01 | |
US07/861,612 | 1992-04-01 | ||
US98135792A | 1992-11-25 | 1992-11-25 | |
US07/981,357 | 1992-11-25 | ||
US4067793A | 1993-03-31 | 1993-03-31 | |
US08/040,677 | 1993-03-31 | ||
PCT/US1993/003141 WO1993020185A1 (en) | 1992-04-01 | 1993-04-01 | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2002507881A true JP2002507881A (ja) | 2002-03-12 |
JP3649335B2 JP3649335B2 (ja) | 2005-05-18 |
Family
ID=27365767
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51773893A Expired - Lifetime JP3649335B2 (ja) | 1992-04-01 | 1993-04-01 | 樹枝状細胞前駆体のインビトロ増殖の方法およびその免疫原製造への使用 |
Country Status (9)
Country | Link |
---|---|
US (5) | US5994126A (ja) |
EP (1) | EP0633929B1 (ja) |
JP (1) | JP3649335B2 (ja) |
AT (1) | ATE260971T1 (ja) |
CA (1) | CA2133409C (ja) |
DE (1) | DE69333433T2 (ja) |
DK (1) | DK0633929T3 (ja) |
ES (1) | ES2215991T3 (ja) |
PT (1) | PT633929E (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003528573A (ja) * | 1999-05-06 | 2003-09-30 | ウエイク・フオレスト・ユニバーシテイ | 免疫応答を導き出す抗原を同定するための組成物および方法 |
JP2007537753A (ja) * | 2004-05-17 | 2007-12-27 | ボード・オブ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・イリノイ | 抗原でパルスされた、二重特異性抗体(BiAb)でコーティングされた樹状細胞の使用 |
JP2016538293A (ja) * | 2013-11-18 | 2016-12-08 | ユニバーシティー オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケーション | 自己免疫療法のための樹状細胞のマイクロスフェアベースの送達およびex vivo操作 |
Families Citing this family (169)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0633929B1 (en) * | 1992-04-01 | 2004-03-03 | The Rockefeller University | METHOD FOR $i(IN VITRO) PROLIFERATION OF DENDRITIC CELL PRECURSORS AND THEIR USE TO PRODUCE IMMUNOGENS |
US20020085993A1 (en) * | 1992-11-25 | 2002-07-04 | Ralph M. Steinman | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens |
US6300090B1 (en) * | 1994-07-29 | 2001-10-09 | The Rockefeller University | Methods of use of viral vectors to deliver antigen to dendritic cells |
US20010012632A1 (en) * | 1995-03-31 | 2001-08-09 | Muriel Moser | Dendritic-like cell/tumor cell hybrids and hybridomas for inducing an anti-tumor response |
US5871728A (en) * | 1995-03-31 | 1999-02-16 | University Of Pittsburgh | Method of regulating dendritic cell maturation |
US20020182730A1 (en) * | 1995-07-26 | 2002-12-05 | Micheal L. Gruenberg | Autologous immune cell therapy: cell compositions, methods and applications to treatment of human disease |
US7150992B1 (en) * | 1995-10-04 | 2006-12-19 | Innunex Corporation | Methods of preparing dendritic cells with flt3-ligand and antigen |
US6080409A (en) * | 1995-12-28 | 2000-06-27 | Dendreon Corporation | Immunostimulatory method |
US7659119B2 (en) | 1996-02-12 | 2010-02-09 | Argos Therapeutics, Inc. | Method and compositions for obtaining mature dendritic cells |
EP0808897A1 (en) * | 1996-05-21 | 1997-11-26 | I.D.M. Immuno-Designed Molecules | New antigen presenting cells, a process for preparing the same and their use as cellular vaccines |
EP0961824A4 (en) * | 1996-07-10 | 2003-01-29 | Immunex Corp | DENDRITIC CELL ACTIVATION PROCESS |
US6458585B1 (en) | 1996-08-14 | 2002-10-01 | Nexell Therapeutics Inc. | Cytokine-free culture of dendritic cells |
US6977073B1 (en) | 1997-02-07 | 2005-12-20 | Cem Cezayirli | Method for stimulating an immune response |
US6251665B1 (en) | 1997-02-07 | 2001-06-26 | Cem Cezayirli | Directed maturation of stem cells and production of programmable antigen presenting dentritic cells therefrom |
US20040110283A1 (en) * | 1997-02-27 | 2004-06-10 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Induction of tumor and viral immunity using antigen presenting cell co-culture products and fusion products |
US20050169898A1 (en) * | 1997-04-15 | 2005-08-04 | Jianlin Gong | Cell fusions and methods of making and using the same |
AU744042B2 (en) | 1997-04-15 | 2002-02-14 | Dana-Farber Cancer Institute, Inc. | Dendritic cell hybrids |
WO1998053048A1 (en) * | 1997-05-21 | 1998-11-26 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods and compositions for making dendritic cells from expanded populations of monocytes and for activating t cells |
US6274378B1 (en) | 1997-10-27 | 2001-08-14 | The Rockefeller University | Methods and compositions for obtaining mature dendritic cells |
AU763719B2 (en) | 1997-12-08 | 2003-07-31 | Merck Patent Gmbh | Heterodimeric fusion proteins useful for targeted immune therapy and general immune stimulation |
US6602709B1 (en) * | 1998-02-20 | 2003-08-05 | The Rockefeller University | Methods for use of apoptotic cells to deliver antigen to dendritic cells for induction or tolerization of T cells |
US20020034819A1 (en) * | 1998-02-23 | 2002-03-21 | Alan K. Smith | Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses |
US7402307B2 (en) * | 1998-03-31 | 2008-07-22 | Geron Corporation | Method for identifying and killing cancer cells |
WO1999050392A1 (en) | 1998-03-31 | 1999-10-07 | Geron Corporation | Methods and compositions for eliciting an immune response to a telomerase antigen |
US6835550B1 (en) * | 1998-04-15 | 2004-12-28 | Genencor International, Inc. | Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins |
US6936249B1 (en) * | 1998-04-15 | 2005-08-30 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
US6921751B1 (en) * | 1998-05-20 | 2005-07-26 | Erasmus Universiteit Rotterdam | Immunoregulator |
US8680059B2 (en) * | 1998-05-20 | 2014-03-25 | Biotempt B.V. | Oligopeptide acetate and formulations thereof |
US6844315B2 (en) | 1998-05-20 | 2005-01-18 | Erasmus Universiteit Rotterdam | Immunoregulator |
EP1138692A1 (en) * | 2000-03-29 | 2001-10-04 | Erasmus Universiteit Rotterdam | Fragments of human chorionic gonadotropin (hcg) as immunoregulator |
US20030220258A1 (en) | 2001-12-21 | 2003-11-27 | Robbert Benner | Treatment of ischemic events |
US20040202645A1 (en) * | 2003-04-08 | 2004-10-14 | Khan Nisar Ahmed | Administration of gene-regulatory peptides |
US20050227925A1 (en) * | 2004-04-08 | 2005-10-13 | Robbert Benner | Compositions capable of reducing elevated blood urea concentration |
US20020051783A1 (en) * | 1998-06-05 | 2002-05-02 | Savage Philip Michael | Method for producing or enhancing a T-cell response against a target cell using a complex comprising an HLA class I molecule and an attaching means |
US7521197B2 (en) * | 1998-06-05 | 2009-04-21 | Alexis Biotech Limited | Method for producing cytotoxic T-cells |
US7264965B2 (en) * | 1998-06-05 | 2007-09-04 | Alexis Biotech Limited | Method for producing or enhancing a T-cell response against a target cell using a complex comprising an HLA class I molecule and an attaching means |
US6558951B1 (en) * | 1999-02-11 | 2003-05-06 | 3M Innovative Properties Company | Maturation of dendritic cells with immune response modifying compounds |
CA2367692A1 (en) * | 1999-03-15 | 2000-09-21 | Introgen Therapeutics, Inc. | Dendritic cells transduced with a wild-type self gene elicit potent antitumor immune responses |
US20020090381A1 (en) * | 1999-04-09 | 2002-07-11 | H. Kim Bottomly | System for controlling immune system response to antigen |
US6977245B2 (en) | 1999-04-12 | 2005-12-20 | The United States Of America As Represented By The Department Of Health And Human Services | Oligodeoxynucleotide and its use to induce an immune response |
EP1175497B1 (en) | 1999-04-14 | 2010-04-07 | Novartis Vaccines and Diagnostics, Inc. | Compositions and methods for generating an immune response utilizing alphavirus-based vector systems |
SK782002A3 (en) | 1999-07-21 | 2003-08-05 | Lexigen Pharm Corp | FC fusion proteins for enhancing the immunogenicity of protein and peptide antigens |
US7067110B1 (en) | 1999-07-21 | 2006-06-27 | Emd Lexigen Research Center Corp. | Fc fusion proteins for enhancing the immunogenicity of protein and peptide antigens |
US7713739B1 (en) | 2000-11-17 | 2010-05-11 | Novartis Vaccines And Diagnostics, Inc. | Microparticle-based transfection and activation of dendritic cells |
CA2380331C (en) | 1999-08-09 | 2012-11-20 | Lexigen Pharmaceuticals Corp. | Multiple cytokine-antibody complexes |
US7005131B1 (en) * | 1999-08-13 | 2006-02-28 | The Rockefeller University | Protective antigen of Epstein Barr Virus |
AU782645B2 (en) | 1999-08-31 | 2005-08-18 | University Of North Carolina At Chapel Hill, The | Antibody dependent enhancement (ADE) of alphavirus infection |
DK1224264T3 (da) | 1999-10-15 | 2010-05-10 | Baylor Res Inst | Anvendelse af allogene cellelinjer til at belaste antigenpræsenterende celler til at fremkomme med immunresponser |
US20030077263A1 (en) * | 1999-10-29 | 2003-04-24 | Immunex Corporation | Method of activating dendritic cells |
CA2391080A1 (en) | 1999-11-12 | 2001-05-25 | Merck Patent Gesellschaft Mit Beschraenkter Haftung | Erythropoietin forms with improved properties |
US7030228B1 (en) | 1999-11-15 | 2006-04-18 | Miltenyi Biotec Gmbh | Antigen-binding fragments specific for dendritic cells, compositions and methods of use thereof antigens recognized thereby and cells obtained thereby |
CA2394831A1 (en) * | 2000-01-11 | 2001-07-19 | Maxygen, Inc. | Monocyte-derived dendritic cell subsets |
DE60131430T2 (de) * | 2000-01-14 | 2008-10-16 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Oligodeoxynukleotide und ihre verwendung zur induktion einer immunreaktion |
CA2399832C (en) | 2000-02-11 | 2011-09-20 | Stephen D. Gillies | Enhancing the circulating half-life of antibody-based fusion proteins |
US7541184B2 (en) | 2000-02-24 | 2009-06-02 | Invitrogen Corporation | Activation and expansion of cells |
AU2001239873A1 (en) | 2000-02-24 | 2001-09-03 | The Board Of Trustees Of The Leland Stanford Junior University | Adjuvant treatment by in vivo activation of dendritic cells |
US7572631B2 (en) | 2000-02-24 | 2009-08-11 | Invitrogen Corporation | Activation and expansion of T cells |
WO2001062092A1 (en) * | 2000-02-25 | 2001-08-30 | Thomas Jefferson University | Formulations and methods for using the same to elicit an immune response |
EP1300418A1 (en) * | 2001-10-04 | 2003-04-09 | Erasmus Universiteit Rotterdam | Gene regulation by oligopeptides |
US7358330B2 (en) * | 2001-03-29 | 2008-04-15 | Biotempt B.V. | Immunoregulatory compositions |
US7361329B2 (en) * | 2000-04-21 | 2008-04-22 | Chemocentryx, Inc. | Compositions for inducing an immune response |
WO2001087325A1 (en) * | 2000-05-12 | 2001-11-22 | Northwest Biotherapeutics, Inc. | Method to increase class i presentation of exogenous antigens by human dendritic cells |
AU2001271729B2 (en) | 2000-06-29 | 2007-01-04 | Merck Patent Gmbh | Enhancement of antibody-cytokine fusion protein mediated immune responses by combined treatment with immunocytokine uptake enhancing agents |
AU2524502A (en) * | 2000-10-13 | 2002-04-22 | Richard Boyd | Improvement of graft acceptance through manipulation of thymic regeneration |
US6892140B1 (en) * | 2000-11-27 | 2005-05-10 | Enteron, Inc. | Immunogenic cancer peptides and uses thereof |
US20020094545A1 (en) * | 2000-11-30 | 2002-07-18 | Harris Paul E. | Growth of human dendritic cells for cancer immunotherapy in closed system using microcarrier beads |
EP1351969A4 (en) * | 2000-12-13 | 2006-04-05 | Immunex Corp | PROCESS RELATING TO THE PRODUCTION OF IMMORTAL DENDRITIC CELL LINES |
US20030027334A1 (en) * | 2000-12-13 | 2003-02-06 | Fitzpatrick David R. | Method for generating immortal dendritic cell lines |
KR20090010127A (ko) | 2001-03-07 | 2009-01-28 | 메르크 파텐트 게엠베하 | 하이브리드 이소타입 항체 부분구조를 포함하는 단백질을 위한 발현 기술 |
FR2821947B1 (fr) * | 2001-03-12 | 2003-05-16 | Canon Kk | Procede et dispositif de validation de parametres definissant une image |
US20020131953A1 (en) * | 2001-03-14 | 2002-09-19 | Ut Southwestern Medical Center | In situ langerhans cell vaccine |
US20030165479A1 (en) * | 2001-03-19 | 2003-09-04 | Velleca Mark A. | Methods for isolating proteins expressed by dendritic cells |
WO2002079415A2 (en) | 2001-03-30 | 2002-10-10 | Lexigen Pharmaceuticals Corp. | Reducing the immunogenicity of fusion proteins |
AU2002249531A1 (en) * | 2001-04-02 | 2002-10-28 | Alimentary Health Limited | Immunotherapy based on dendritic cells |
US20020193295A1 (en) * | 2001-05-04 | 2002-12-19 | Emanuel Calenoff | Immunogenic peptides and uses thereof |
US20030082806A1 (en) * | 2001-04-27 | 2003-05-01 | Xcyte Therapies, Inc. | Maturation of antigen-presenting cells using activated T cells |
KR20020083634A (ko) * | 2001-04-27 | 2002-11-04 | 크레아젠 주식회사 | 수지상 세포를 포함하는 자가면역 질환의 치료용 약제학적조성물 및 이를 이용한 치료방법 |
DE60239454D1 (de) | 2001-05-03 | 2011-04-28 | Merck Patent Gmbh | Rekombinanter, tumorspezifischer antikörper und dessen verwendung |
WO2003010292A2 (en) * | 2001-07-25 | 2003-02-06 | Northwest Biotherapeutics, Inc. | Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors |
US7666674B2 (en) | 2001-07-27 | 2010-02-23 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Use of sterically stabilized cationic liposomes to efficiently deliver CPG oligonucleotides in vivo |
AU2002361468A1 (en) | 2001-08-14 | 2003-03-18 | The Government Of The United States Of America As Represented By The Secretary Of Health And Human S | Method for rapid generation of mature dendritic cells |
US20030134341A1 (en) * | 2001-09-19 | 2003-07-17 | Medcell Biologics, Llc. | Th1 cell adoptive immunotherapy |
US20030134415A1 (en) * | 2001-09-19 | 2003-07-17 | Gruenberg Micheal L. | Th1 cell adoptive immunotherapy |
US20030220259A1 (en) * | 2001-12-21 | 2003-11-27 | Robbert Benner | Treatment of neurological disorders |
US20030215460A1 (en) * | 2002-05-07 | 2003-11-20 | Schall Thomas J. | Methods and compositions for inducing an immune response |
US20030096260A1 (en) * | 2001-10-09 | 2003-05-22 | Zhenhua Miao | Compositions useful as ligands for the formyl peptide receptor like 1 receptor and methods of use thereof |
PT1454138E (pt) | 2001-12-04 | 2012-03-28 | Merck Patent Gmbh | Imunocitoquinas com seletividade modulada |
US8466116B2 (en) | 2001-12-20 | 2013-06-18 | The Unites States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Use of CpG oligodeoxynucleotides to induce epithelial cell growth |
AU2002366710A1 (en) | 2001-12-20 | 2003-07-09 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of | USE OF CpG OLIGODEOXYNUCLEOTIDES TO INDUCE ANGIOGENESIS |
US20030220261A1 (en) * | 2001-12-21 | 2003-11-27 | Khan Nisar Ahmed | Treatment of iatrogenic disease |
US20030224995A1 (en) * | 2001-12-21 | 2003-12-04 | Khan Nisar Ahmed | Treatment of burns |
US20040013661A1 (en) * | 2001-12-21 | 2004-01-22 | Gert Wensvoort | Stratification |
US20030220257A1 (en) * | 2001-12-21 | 2003-11-27 | Robbert Benner | Treatment of trauma |
US20030220260A1 (en) * | 2001-12-21 | 2003-11-27 | Khan Nisar Ahmed | Peptide compositions |
US20080242837A1 (en) * | 2001-12-21 | 2008-10-02 | Khan Nisar A | Peptide compositions |
US20080318871A1 (en) * | 2001-12-21 | 2008-12-25 | Khan Nisar A | Treatment of neurological disorders |
US7786084B2 (en) * | 2001-12-21 | 2010-08-31 | Biotempt B.V. | Treatment of burns |
US7501391B2 (en) * | 2001-12-21 | 2009-03-10 | Biotempt B.V. | Treatment of transplant survival |
US7560433B2 (en) * | 2001-12-21 | 2009-07-14 | Biotempt B.V. | Treatment of multiple sclerosis (MS) |
US20040230380A1 (en) * | 2002-01-04 | 2004-11-18 | Xencor | Novel proteins with altered immunogenicity |
US20030149646A1 (en) * | 2002-02-01 | 2003-08-07 | Ubs Painewebber Inc. | Method and system for providing an aggregated stock options report |
US20030175272A1 (en) * | 2002-03-07 | 2003-09-18 | Medcell Biologics, Inc. | Re-activated T-cells for adoptive immunotherapy |
US7060494B2 (en) * | 2002-04-09 | 2006-06-13 | Reliance Life Sciences Pvt. Ltd. | Growth of human Mesenchymal Stem Cells (hMSC) using umbilical cord blood serum and the method for the preparation thereof |
US20060292618A1 (en) * | 2002-04-12 | 2006-12-28 | Mellor Andrew L | Antigen-presenting cell populations and their use as reagents for enhancing or reducing immune tolerance |
EP1507868B1 (en) * | 2002-05-08 | 2011-11-16 | NorthWest Biotherapeutics, Inc. | Quality assays for antigen presenting cells |
US7048922B2 (en) * | 2002-05-29 | 2006-05-23 | Demao Yang | Stimulation of hematopoiesis by ex vivo activated immune cells |
US7332158B2 (en) * | 2002-05-29 | 2008-02-19 | Demao Yang | Compositions and treatments for myelosuppression by ex vivo activated immune cells |
CA2388441A1 (en) * | 2002-06-10 | 2003-12-10 | Wei-Ping Min | Immunomodulation using rna interference |
US7465448B2 (en) * | 2002-09-11 | 2008-12-16 | Medical College Of Georgia Research Institute, Inc. | Chemokine receptor antagonists as therapeutic agents |
US8263091B2 (en) | 2002-09-18 | 2012-09-11 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Method of treating and preventing infections in immunocompromised subjects with immunostimulatory CpG oligonucleotides |
US7413857B2 (en) | 2002-11-21 | 2008-08-19 | Epicentre Technologies | Methods for using riboprimers for strand displacement replication of target sequences |
MXPA05006042A (es) | 2002-12-06 | 2005-09-21 | Northwest Biotherapeutics Inc | Administracion de celulas dendriticas parcialmente maduradas in-vitro para el tratamiento de tumores. |
RU2366664C2 (ru) | 2002-12-17 | 2009-09-10 | Мерк Патент Гмбх | Гуманизированное антитело (н14.18) на основании антитела 14.18 мыши, связывающееся с gd2, и его слияние с il-2 |
AU2004206853A1 (en) * | 2003-01-16 | 2004-08-05 | Becton, Dickinson And Company | Intradermal cellular delivery using narrow gauge micro-cannula |
US20040197903A1 (en) * | 2003-01-31 | 2004-10-07 | Northwest Biotherapeutics, Inc. | Method for induction of proliferation of natural killer cells by dendritic cells cultured with GM-CSF and IL-15 |
EP1597356A4 (en) * | 2003-02-10 | 2007-07-11 | Northwest Biotherapeutics Inc | CELLS HAVING THE CD14 + ANTIGEN OF CULTURE |
US20060134067A1 (en) * | 2003-02-18 | 2006-06-22 | Maxcyte, Inc. | Loading of cells with antigens by electroporation |
AU2004258799A1 (en) * | 2003-02-21 | 2005-02-03 | Hasumi Llc (Dba Shukokai International) | Human lymphocyte vaccine adjuvant |
US8389278B2 (en) | 2003-02-27 | 2013-03-05 | Northwest Biotherapeutics, Inc. | Generation of dendritic cells from monocytic dendritic precursor cells with GM-CSF in the absence of additional cytokines |
EP1608728A2 (en) * | 2003-03-31 | 2005-12-28 | University Of South Florida Research Foundation, Inc. | Materials and methods for regulating process formation in cell culture |
US20040203142A1 (en) * | 2003-04-14 | 2004-10-14 | Reliance Life Sciences Pvt. Ltd. | Growth of neural precursor cells using umbilical cord blood serum and a process for the preparation thereof for therapeutic purposes |
EP1688483A4 (en) * | 2003-07-22 | 2007-09-12 | Kirin Brewery | METHOD FOR PRODUCING A LANGERHANSSCHEN CELL FROM A CD14 POSITIVE CELL WHICH IS A HUMAN MONONUCLEAR PERIPHERAL BLOOD CELL USING THE NOTCH LIGAND DELTA-1, GM-CSF AND TGF-BETA |
EP1670513B1 (en) * | 2003-10-06 | 2013-01-23 | Cedars-Sinai Medical Center | Cox-2 inhibitors and dendritic cells for use in the treatment of cancer. |
JP5015601B2 (ja) * | 2003-10-21 | 2012-08-29 | セダーズ−シナイ メディカル センター | 中枢神経系の癌を含む癌の処置のための系および方法 |
US20090227505A1 (en) * | 2004-01-07 | 2009-09-10 | Biotempt B.V. | Methods and uses for protein breakdown products |
US20070087986A1 (en) * | 2004-01-26 | 2007-04-19 | Brett Premack | Compositions and methods for enhancing immunity by chemoattractant adjuvants |
WO2005077412A2 (en) * | 2004-01-26 | 2005-08-25 | Chemocentryx | Compositions and methods of use of w-peptides |
US20050214268A1 (en) * | 2004-03-25 | 2005-09-29 | Cavanagh William A Iii | Methods for treating tumors and cancerous tissues |
US7655393B2 (en) * | 2004-05-11 | 2010-02-02 | Shukokai Incorporated | Adoptive immune cells for tumor vaccines |
US7862829B2 (en) * | 2004-07-09 | 2011-01-04 | University Of North Carolina At Chapel Hill | Viral adjuvants |
CA2504451A1 (en) * | 2004-08-10 | 2006-02-10 | Geron Corporation | Dendritic cell vaccines for treating cancer made from embryonic stem cells |
US20060057121A1 (en) * | 2004-09-10 | 2006-03-16 | Demao Yang | Compositions and treatments using ex vivo activated cells for myelosuppressed patients |
DE602005025005D1 (de) | 2004-10-07 | 2011-01-05 | Argos Therapeutics Inc | Zusammensetzungen reifer dendritischer zellen und verfahren zu deren kultivierung |
JP2008520684A (ja) * | 2004-11-17 | 2008-06-19 | アブジェニックス・インコーポレーテッド | Il−13に対する完全ヒトモノクローナル抗体 |
US20090028876A1 (en) * | 2005-01-24 | 2009-01-29 | University Of Rochester | Methods of modifying cd11c+ dendritic cell development to form osteoclasts functional in the bone environment |
CA2597921A1 (en) * | 2005-02-15 | 2007-04-26 | University Of North Carolina At Chapel Hill | New live virus vaccines |
JP2008539751A (ja) * | 2005-05-10 | 2008-11-20 | アヴァリス・アーベー | 新規組成物およびその使用 |
AU2006249199A1 (en) * | 2005-05-12 | 2006-11-23 | Introgen Therapeutics, Inc. | P53 vaccines for the treatment of cancers |
AU2006266609C1 (en) * | 2005-07-05 | 2011-10-27 | Biotempt B.V. | Treatment of tumors |
JP5954918B2 (ja) | 2005-12-08 | 2016-07-20 | ノースウエスト バイオセラピューティクス, インコーポレイテッド | 未成熟な単球性樹状細胞の活性化を誘導するための組成物および方法 |
EP1864692A1 (en) * | 2006-06-07 | 2007-12-12 | Biotempt B.V. | Use of peptides for the control of radiation injury |
WO2007137300A2 (en) | 2006-05-23 | 2007-11-29 | Bellicum Pharmaceuticals, Inc. | Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods |
JP2009542714A (ja) * | 2006-06-30 | 2009-12-03 | ベイラー リサーチ インスティテュート | GM−CSF及びインターフェロンαを用いて生成され、且つ熱処理され死滅したがん細胞を取り込んだ樹状細胞 |
US8129184B2 (en) * | 2006-09-26 | 2012-03-06 | Cedars-Sinai Medical Center | Cancer stem cell antigen vaccines and methods |
US8097256B2 (en) | 2006-09-28 | 2012-01-17 | Cedars-Sinai Medical Center | Cancer vaccines and vaccination methods |
GB0622399D0 (en) * | 2006-11-10 | 2006-12-20 | Avaris Ab | Novel compositions and uses thereof |
US20080286315A1 (en) * | 2007-01-31 | 2008-11-20 | Penta Biotech, Inc. | Methionine Enkephalin as an Adjuvant for Vaccine Immunizations |
DE102007006736B4 (de) | 2007-02-07 | 2012-01-12 | Dagmar Briechle | In-vitro Testkit zur tierversuchsfreien Bestimmung des sensibilisierenden Potentials einer Substanz |
KR100887560B1 (ko) * | 2007-04-24 | 2009-03-09 | 크레아젠 주식회사 | 준성숙 수지상 세포를 포함하는 류마티스 관절염 치료용약제학적 조성물 |
WO2009013018A2 (en) * | 2007-07-24 | 2009-01-29 | Stichting Katholieke Universiteit Radboud University Nijmegen Medical Centre | Compositions and methods for generating an immune response in a subject |
US8513208B2 (en) | 2008-02-28 | 2013-08-20 | Argos Therapeutics, Inc. | Transient expression of immunomodulatory polypeptides for the prevention and treatment of autoimmune disease, allergy and transplant rejection |
US9068020B2 (en) * | 2008-09-02 | 2015-06-30 | Cedars-Sinai Medical Center | CD133 epitopes |
WO2010129895A2 (en) * | 2009-05-07 | 2010-11-11 | Immunocellular Therapeutics, Ltd. | Cd133 epitopes |
EP2281579A1 (en) | 2009-08-05 | 2011-02-09 | BioNTech AG | Vaccine composition comprising 5'-Cap modified RNA |
WO2012115276A1 (en) * | 2011-02-23 | 2012-08-30 | Kyoto University | Method for producing dendritic cells from pluripotent stem cells |
JP5577472B2 (ja) * | 2012-02-10 | 2014-08-20 | 医療法人社団博心厚生会 | 単球増殖剤、単球増殖用培地、単球の製造方法、樹状細胞の製造方法、及び樹状細胞ワクチンの製造方法 |
WO2014127296A1 (en) | 2013-02-14 | 2014-08-21 | Immunocellular Therapeutics, Ltd | Cancer vaccines and vaccination methods |
KR101807778B1 (ko) | 2014-06-23 | 2018-01-18 | 제이더블유크레아젠 주식회사 | 특정 유전자 발현이 증가된 수지상세포의 제조방법 및 이를 이용하여 제조된 수지상세포를 포함하는 자가면역질환 치료 또는 예방용 조성물 |
EP3297680A2 (en) | 2015-05-20 | 2018-03-28 | Yeda Research and Development Co. Ltd | Method of targeting senescent cells |
US20170216354A1 (en) * | 2015-06-12 | 2017-08-03 | Batu Biologics, Inc. | Clinically useful non-antigen pulsed dendritic cells |
KR20180044430A (ko) | 2015-09-15 | 2018-05-02 | 노쓰웨스트 바이오써라퓨틱스, 인크. | 진행성 암을 가진 대상체를 위한 활성화된 수지상 세포 조성물 및 면역요법적 치료와 관련된 방법 |
EP3377635A4 (en) | 2015-11-18 | 2019-05-08 | Orbis Health Solutions LLC | VIRAL VECTOR SYSTEM ALPHA T7 |
AU2017283480A1 (en) | 2016-06-13 | 2019-01-24 | Torque Therapeutics, Inc. | Methods and compositions for promoting immune cell function |
US11364264B2 (en) | 2016-09-09 | 2022-06-21 | The General Hospital Corporation | Ex vivo antigen-presenting cells or activated CD-positive T cells for treatment of cancer |
WO2018071576A1 (en) | 2016-10-14 | 2018-04-19 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Treatment of tumors by inhibition of cd300f |
WO2018213803A1 (en) | 2017-05-19 | 2018-11-22 | Neon Therapeutics, Inc. | Immunogenic neoantigen identification |
AU2018328209A1 (en) | 2017-09-05 | 2020-04-23 | Torque Therapeutics, Inc. | Therapeutic protein compositions and methods of making and using the same |
CA3081840A1 (en) | 2017-11-08 | 2019-05-16 | Neon Therapeutics, Inc. | T cell manufacturing compositions and methods |
CN112243382A (zh) | 2018-01-18 | 2021-01-19 | 南佛罗里达大学 | 死抗原刺激的未成熟异源性树突细胞作为疾病治疗剂 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5130144B1 (en) * | 1984-02-06 | 1995-08-15 | Univ Johns Hopkins | Human stem cells and monoclonal antibodies |
US4861589A (en) * | 1987-03-23 | 1989-08-29 | Trustees Of Boston University | Method for therapeutically treating abnormal cells expressing a major histocompatibility complex class II antigen using cytolytic inducer T4 cells |
US20030072751A1 (en) * | 1990-03-14 | 2003-04-17 | Heribert Bohlen | Idiotypic vaccination against b cell lymphoma |
US5364783A (en) * | 1990-05-14 | 1994-11-15 | Massachusetts Institute Of Technology | Retrovirus promoter-trap vectors |
CA2084180A1 (en) * | 1991-12-11 | 1993-06-12 | Paul P. Hung | Expression of specific immunogens using viral antigens |
EP0563485A1 (en) * | 1992-03-30 | 1993-10-06 | Schering-Plough | In vitro generation of human dendritic cells and uses thereof |
AU687733B2 (en) * | 1992-04-01 | 1998-03-05 | Rockefeller University, The | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens |
EP0633929B1 (en) * | 1992-04-01 | 2004-03-03 | The Rockefeller University | METHOD FOR $i(IN VITRO) PROLIFERATION OF DENDRITIC CELL PRECURSORS AND THEIR USE TO PRODUCE IMMUNOGENS |
AU4678993A (en) * | 1992-07-16 | 1994-02-14 | Board Of Trustees Of The Leland Stanford Junior University | Methods for using dendritic cells to activate t cells |
US20020085993A1 (en) * | 1992-11-25 | 2002-07-04 | Ralph M. Steinman | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens |
WO1995015340A1 (en) * | 1993-12-03 | 1995-06-08 | The Board Of Trustees Of Leland Stanford Junior University | Monoclonal antibodies to antigens expressed by human dendritic cells |
DE4412794A1 (de) * | 1994-04-14 | 1995-12-14 | Univ Ludwigs Albert | Verfahren zur Herstellung von dendritischen Zellen, so erhaltene Zellen und Behälter zur Durchführung dieses Verfahrens |
EP0765386B1 (en) * | 1994-06-14 | 2014-12-10 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for in vivo t cell activation by antigen-pulsed dendritic cells |
US7659119B2 (en) * | 1996-02-12 | 2010-02-09 | Argos Therapeutics, Inc. | Method and compositions for obtaining mature dendritic cells |
US6274378B1 (en) * | 1997-10-27 | 2001-08-14 | The Rockefeller University | Methods and compositions for obtaining mature dendritic cells |
-
1993
- 1993-04-01 EP EP93911581A patent/EP0633929B1/en not_active Expired - Lifetime
- 1993-04-01 AT AT93911581T patent/ATE260971T1/de active
- 1993-04-01 DK DK93911581T patent/DK0633929T3/da active
- 1993-04-01 DE DE69333433T patent/DE69333433T2/de not_active Expired - Lifetime
- 1993-04-01 PT PT93911581T patent/PT633929E/pt unknown
- 1993-04-01 CA CA2133409A patent/CA2133409C/en not_active Expired - Lifetime
- 1993-04-01 ES ES93911581T patent/ES2215991T3/es not_active Expired - Lifetime
- 1993-04-01 JP JP51773893A patent/JP3649335B2/ja not_active Expired - Lifetime
-
1994
- 1994-06-17 US US08/261,537 patent/US5994126A/en not_active Expired - Lifetime
-
1995
- 1995-06-02 US US08/458,230 patent/US5851756A/en not_active Expired - Lifetime
-
1999
- 1999-11-30 US US09/451,511 patent/US6475483B1/en not_active Expired - Fee Related
-
2002
- 2002-11-05 US US10/287,813 patent/US20030134419A1/en not_active Abandoned
-
2008
- 2008-07-17 US US12/218,899 patent/US7923247B2/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003528573A (ja) * | 1999-05-06 | 2003-09-30 | ウエイク・フオレスト・ユニバーシテイ | 免疫応答を導き出す抗原を同定するための組成物および方法 |
JP2007537753A (ja) * | 2004-05-17 | 2007-12-27 | ボード・オブ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・イリノイ | 抗原でパルスされた、二重特異性抗体(BiAb)でコーティングされた樹状細胞の使用 |
JP2016538293A (ja) * | 2013-11-18 | 2016-12-08 | ユニバーシティー オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケーション | 自己免疫療法のための樹状細胞のマイクロスフェアベースの送達およびex vivo操作 |
Also Published As
Publication number | Publication date |
---|---|
US20030134419A1 (en) | 2003-07-17 |
US6475483B1 (en) | 2002-11-05 |
PT633929E (pt) | 2004-07-30 |
CA2133409A1 (en) | 1993-10-14 |
EP0633929A1 (en) | 1995-01-18 |
EP0633929B1 (en) | 2004-03-03 |
CA2133409C (en) | 2011-05-24 |
ATE260971T1 (de) | 2004-03-15 |
US5851756A (en) | 1998-12-22 |
US20090029469A1 (en) | 2009-01-29 |
DK0633929T3 (da) | 2004-06-28 |
US7923247B2 (en) | 2011-04-12 |
JP3649335B2 (ja) | 2005-05-18 |
US5994126A (en) | 1999-11-30 |
DE69333433D1 (de) | 2004-04-08 |
ES2215991T3 (es) | 2004-10-16 |
DE69333433T2 (de) | 2004-12-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2002507881A (ja) | 樹枝状細胞前駆体のインビトロ増殖の方法およびその免疫原製造への使用 | |
AU687733B2 (en) | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens | |
Wagner et al. | Cell-mediated immune response in vitro: II. The role of thymus and thymus-derived lymphocytes | |
JP2547162B2 (ja) | 哺乳動物における異系移植片拒否反応を抑制するための方法と組成物 | |
US10881692B2 (en) | Compositions for establishing mixed chimerism and methods of manufacture thereof | |
US8563308B2 (en) | Culture-expanded T suppressor cells and methods of use thereof | |
JP5549014B2 (ja) | 免疫調節剤及びその利用 | |
US20020085993A1 (en) | Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens | |
US20150353897A1 (en) | Method of generating multilineage potential cells | |
JP3917652B2 (ja) | 造血促進細胞及びその使用 | |
US20030165479A1 (en) | Methods for isolating proteins expressed by dendritic cells | |
WO2021236711A1 (en) | Calcineurin inhibitor to improve cd3+cell survival to thereby facilitate engraftment of donor cd34+ cells in a recipient | |
Rapaport et al. | Implication of Ia-positive bone marrow interstitial stem cells in the induction of unresponsiveness to canine renal allografts | |
Rigby | Intestinal dendritic cells: Characterisation of the colonic dendritic cell population and identification of potential precursors | |
BEARDSLEY | 188. Mechanism of action of a cloned thymic epithelial (TEPI) cell line | |
JP2003033175A (ja) | 選択的免疫応答抑制を誘導する末梢血樹状細胞サブセット | |
Williams | Biology of Adult Human Microglia Studied in Vitro and in Situ: Immune Accessory and Effector Functions | |
IL106295A (en) | Hematopoietic facilitaroy cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20040311 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20040426 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20040616 |
|
A72 | Notification of change in name of applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A721 Effective date: 20041104 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20050118 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20050210 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080225 Year of fee payment: 3 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090225 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100225 Year of fee payment: 5 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100225 Year of fee payment: 5 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110225 Year of fee payment: 6 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120225 Year of fee payment: 7 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120225 Year of fee payment: 7 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130225 Year of fee payment: 8 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130225 Year of fee payment: 8 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140225 Year of fee payment: 9 |