JP2002500042A - 非同一遺伝子ならびに改良された分子アジュバントにおけるそれらの適用 - Google Patents
非同一遺伝子ならびに改良された分子アジュバントにおけるそれらの適用Info
- Publication number
- JP2002500042A JP2002500042A JP2000527645A JP2000527645A JP2002500042A JP 2002500042 A JP2002500042 A JP 2002500042A JP 2000527645 A JP2000527645 A JP 2000527645A JP 2000527645 A JP2000527645 A JP 2000527645A JP 2002500042 A JP2002500042 A JP 2002500042A
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- Prior art keywords
- polypeptide
- concatemer
- dna
- sequence
- encoding
- Prior art date
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- Pending
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- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
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- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 230000000899 immune system response Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
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- 210000004969 inflammatory cell Anatomy 0.000 description 1
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- 238000011068 loading method Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
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- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
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- 238000002156 mixing Methods 0.000 description 1
- 108091005601 modified peptides Proteins 0.000 description 1
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- 108091005763 multidomain proteins Proteins 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 230000014207 opsonization Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
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- 238000010979 pH adjustment Methods 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
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- 239000002245 particle Substances 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 description 1
- 229910000073 phosphorus hydride Inorganic materials 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000007694 polyacrylamide gel isoelectric focusing Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- 235000019419 proteases Nutrition 0.000 description 1
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- 238000001742 protein purification Methods 0.000 description 1
- 230000016434 protein splicing Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- WHMDPDGBKYUEMW-UHFFFAOYSA-N pyridine-2-thiol Chemical compound SC1=CC=CC=N1 WHMDPDGBKYUEMW-UHFFFAOYSA-N 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
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- 108020003175 receptors Proteins 0.000 description 1
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- 238000011084 recovery Methods 0.000 description 1
- 239000006176 redox buffer Substances 0.000 description 1
- 108091035233 repetitive DNA sequence Proteins 0.000 description 1
- 102000053632 repetitive DNA sequence Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
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- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
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- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
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- 230000009261 transgenic effect Effects 0.000 description 1
- ZGYICYBLPGRURT-UHFFFAOYSA-N tri(propan-2-yl)silicon Chemical compound CC(C)[Si](C(C)C)C(C)C ZGYICYBLPGRURT-UHFFFAOYSA-N 0.000 description 1
- 229940117957 triethanolamine hydrochloride Drugs 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 230000008728 vascular permeability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/472—Complement proteins, e.g. anaphylatoxin, C3a, C5a
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
- C07K2319/715—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16 containing a domain for ligand dependent transcriptional activation, e.g. containing a steroid receptor domain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9727512.7A GB9727512D0 (en) | 1997-12-31 | 1997-12-31 | Fuzzy genes and their application in molecular adjuvants |
| GB9727512.7 | 1997-12-31 | ||
| PCT/GB1998/003918 WO1999035260A1 (en) | 1997-12-31 | 1998-12-30 | Non-identical genes and their application in improved molec ular adjuvants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002500042A true JP2002500042A (ja) | 2002-01-08 |
| JP2002500042A5 JP2002500042A5 (enExample) | 2006-03-09 |
Family
ID=10824333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000527645A Pending JP2002500042A (ja) | 1997-12-31 | 1998-12-30 | 非同一遺伝子ならびに改良された分子アジュバントにおけるそれらの適用 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6770631B1 (enExample) |
| EP (1) | EP1042467B1 (enExample) |
| JP (1) | JP2002500042A (enExample) |
| AT (1) | ATE403721T1 (enExample) |
| AU (1) | AU757021B2 (enExample) |
| CA (1) | CA2317020A1 (enExample) |
| DE (1) | DE69839848D1 (enExample) |
| GB (1) | GB9727512D0 (enExample) |
| NZ (1) | NZ505827A (enExample) |
| WO (1) | WO1999035260A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0008582D0 (en) * | 2000-04-08 | 2000-05-31 | Adprotech Plc | DNA immunization vectors |
| WO2001092295A2 (en) * | 2000-05-30 | 2001-12-06 | University Of Toronto | Ligands for cd21 and compositions thereof for modulating immune responses |
| GB0025229D0 (en) * | 2000-10-14 | 2000-11-29 | Adprotech Ltd | Veterinary immunisation vectors |
| US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
| WO2002059297A2 (en) | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | A library of a collection of cells |
| KR100453877B1 (ko) | 2001-07-26 | 2004-10-20 | 메덱스젠 주식회사 | 연쇄체화에 의한 면역 글로블린 융합 단백질의 제조 방법 및 이 방법에 의해 제조된 ＴＮＦＲ/Ｆｃ 융합 단백질, 상기 단백질을 코딩하는 ＤＮＡ, 상기 ＤＮＡ를 포함하는벡터, 및 상기 벡터에 의한 형질전환체 |
| GB0204154D0 (en) * | 2002-02-22 | 2002-04-10 | Adprotech Ltd | Cat immunisation vectors |
| US20070104726A1 (en) * | 2002-08-14 | 2007-05-10 | Avidis Sa | Multimeric complexes of antigens and adjuvants |
| US7664748B2 (en) * | 2004-07-12 | 2010-02-16 | John Eric Harrity | Systems and methods for changing symbol sequences in documents |
| JP5241012B2 (ja) | 2005-07-15 | 2013-07-17 | ノバルティス アーゲー | Ｐａｍｐｓ、病原体関連分子パターン |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE233273T1 (de) | 1986-11-04 | 2003-03-15 | Protein Polymer Tech Inc | Herstellung synthetischer dns und deren verwendung bei der synthese grosser polypeptide |
| US5641648A (en) * | 1986-11-04 | 1997-06-24 | Protein Polymer Technologies, Inc. | Methods for preparing synthetic repetitive DNA |
| US6018030A (en) * | 1986-11-04 | 2000-01-25 | Protein Polymer Technologies, Inc. | Peptides comprising repetitive units of amino acids and DNA sequences encoding the same |
| JPH0829098B2 (ja) * | 1988-01-05 | 1996-03-27 | 寳酒造株式会社 | 細胞接着活性ポリペプチド |
| WO1993012257A1 (en) | 1991-12-12 | 1993-06-24 | Hybritech Incorporated | Enzymatic inverse polymerase chain reaction library mutagenesis |
| US5861285A (en) * | 1994-09-16 | 1999-01-19 | Tdk Corporation | Fusion protein-bound magnetic particles for recombinant production and magnetic separation of polypeptides of interest |
| US5763733A (en) * | 1994-10-13 | 1998-06-09 | Enzon, Inc. | Antigen-binding fusion proteins |
| GB9424631D0 (en) | 1994-12-06 | 1995-01-25 | Lynxvale Ltd | Modulating the immune response |
| DE19756975A1 (de) * | 1997-12-20 | 1999-06-24 | Hoechst Marion Roussel De Gmbh | Bindungspartner für Inhibitoren von cyclinabhängigen Kinasen und ihre Verwendung zur Suche nach Inhibitoren, zur Diagnose oder zur Therapie einer Erkrankung |
-
1997
- 1997-12-31 GB GBGB9727512.7A patent/GB9727512D0/en not_active Ceased
-
1998
- 1998-12-30 DE DE69839848T patent/DE69839848D1/de not_active Expired - Fee Related
- 1998-12-30 WO PCT/GB1998/003918 patent/WO1999035260A1/en not_active Ceased
- 1998-12-30 EP EP98962638A patent/EP1042467B1/en not_active Expired - Lifetime
- 1998-12-30 NZ NZ505827A patent/NZ505827A/xx unknown
- 1998-12-30 AU AU17762/99A patent/AU757021B2/en not_active Ceased
- 1998-12-30 JP JP2000527645A patent/JP2002500042A/ja active Pending
- 1998-12-30 US US09/582,761 patent/US6770631B1/en not_active Expired - Fee Related
- 1998-12-30 CA CA002317020A patent/CA2317020A1/en not_active Abandoned
- 1998-12-30 AT AT98962638T patent/ATE403721T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2317020A1 (en) | 1999-07-15 |
| NZ505827A (en) | 2003-03-28 |
| EP1042467A1 (en) | 2000-10-11 |
| US6770631B1 (en) | 2004-08-03 |
| WO1999035260A1 (en) | 1999-07-15 |
| AU757021B2 (en) | 2003-01-30 |
| DE69839848D1 (de) | 2008-09-18 |
| ATE403721T1 (de) | 2008-08-15 |
| AU1776299A (en) | 1999-07-26 |
| GB9727512D0 (en) | 1998-02-25 |
| EP1042467B1 (en) | 2008-08-06 |
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