JP2002226356A - Collagen-containing cosmetic composition derived from pigment-free fish skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cosmetic composition having moisture-retaining effect and hair-protecting effect, capable of preventing, relieving and improving drying, chapping, cracking, wrinkles and inflammatory diseases of the skin and effective against dryness of hair, unoiled hair, split hair and breakage of hair and capable of imparting gloss to hair. SOLUTION: A humectant, a chapped skin-improving agent and a hair- protecting agent are each characterized by including a collagen obtained by extraction from pigment-free skin. The cosmetic composition comprises the humectant, the chapped skin-improving agent or/and the hair/protecting agent.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic for hair and a cosmetic for skin, which contains collagen obtained by extracting pigment-free fish skin and gives moisture to the skin and hair. And a composition useful for maintaining and regenerating live and healthy skin or hair. In other words, it is effective for preventing, reducing or improving dryness, rough skin, cracks, red rags, inflammatory diseases, etc. on the skin, and effective for drying, pasatsuki, split ends, cut hair, gloss imparting, etc. for hair. A cosmetic composition.

[0002]

2. Description of the Related Art Conventionally, collagen has been used for artificial skin, bone reconstructing material and the like because of its applicability to the human body, and further applied to cosmetics. At present, the tissue mainly used for obtaining collagen is calf skin, and recently extracted from non-pigmented fish skin and cold-water fish as disclosed in JP-T-Hei 05-511269 and JP-A-09-278639. Collagen is also disclosed.

[0003]

However, in order to obtain collagen from animals such as calves, there are problems such as the burden of processing for removing hair and subcutaneous tissue and the relatively low extraction yield. . Also, by blending it into cosmetics,
Collagen particles were solidified, did not spread, and could not form a sufficient film on skin, hair, and the like.

[0004]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a collagen having more excellent functions and usability, characterized by containing collagen obtained by extracting from pigment-free fish skin. For preventing, reducing or improving dryness, rough skin, cracks, redheads, inflammatory diseases and the like containing moisturizers, hair protectants and the like, and for hair, dryness, pasatsuki, split ends, cuts The object of the present invention has been achieved by providing a cosmetic composition which is effective in providing hair and gloss.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Pigment-free fish skin used in the present invention includes, for example, white-spotted shrimp (Hot-spotted shrimp, Genco, red-spotted shrimp, southern red-spotted shrimp, denpeshita-shirame, sulcus grenco, inosinosita, sardine serrata, murasakisashibame, Black lobster, Chinese lobster, Japanese boll, Black boll,
Azuma flounder, scorpionfish, etc., which are generally called shibirame), shigara flounder (sole), flounder, small flounder (dab), mako flounder, numa flounder, flounder, red flounder (balais), large flounder (turbot), halibut,
Flatfishes such as Japanese flounder and flounder (brill) (fl
Pigment-free skins of fishes that are caught on an industrial basis, such as at fish), locusts, rays, codfish, merlan, bibs, cardines, etc., can be used. The pigment-free fish skin is a skin obtained after industrial separation of pigmented skin and pigment-free skin, and may be any part of the fish body, but is preferably a ventral skin. Is used. The skin may be fresh fish or frozen directly after cutting or immediately after peeling.

The above fish is extracted using an acidic solvent known to those skilled in the art such as an acetic acid solution. Next, a precipitant such as sodium chloride is added, and the collagen is separated from the supernatant by precipitation. If desired, the crosslinks of this collagen may be cleaved. In this case, the treatment can be performed with a weak acid such as sorbic acid, an alkali, or an enzyme such as pepsin. These collagens may be placed again in an acidic solution or freeze-dried, if necessary. Further, these collagens may be modified by succinylation, phthalation, esterification, acylation, or the like to obtain collagen derivatives. The collagen of the present invention may contain gelatin.

[0007] The production conditions are not particularly limited, but may be usually within the range of the boiling point of the solvent at normal temperature and normal pressure. After extraction, filtration or use of an ion exchange resin is carried out.
A solution, paste, gel, or powder may be obtained by decoloring and purifying. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. An activated carbon column or the like may be used, and ordinary means generally applied to the extracted substance may be arbitrarily selected.

The product of the present invention may be optionally adjusted by drying, concentrating, diluting, etc., depending on the type and form of the moisturizing agent, skin roughness improving agent, hair protecting agent and cosmetic composition to be applied.

In the moisturizing agent, skin roughness improving agent, hair protecting agent and cosmetic composition of the present invention, the product of the present invention contains about 0.001 to 20% by weight, preferably about 0.01 to 10% by weight as a solid content. And good usability and good effects. In the bath composition, the concentration in the bath water is 0.0000
It is preferable to set a single use amount of 1 to 0.01% by weight, preferably about 0.0005 to 0.005% by weight.

The cosmetic composition according to the present invention can be produced by using, in addition to the product of the present invention, bases, additives and the like usually used in cosmetics and the like as described below.
The content in the formulation is not specified, but usually 0.001
~ 20% is preferred.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )etc.

(2) waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid And synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) alcohols ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and phenoxyethanol;
Synthetic alcohols such as hexyldecanol, isostearyl alcohol and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) gum, sugar or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or an ester thereof, trehalose or a derivative thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) to which an alkylene (C2-C4) oxide such as ethylene oxide is added. )
Chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfate or its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose , Carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide and polypropylene oxide Or its cross-linked polymer, carboxyvinyl polymer, polyethylene N'imin like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester nonionic surfactants, ester nonionic surfactants, block polymer nonionic surfactants, nitrogen-containing nonionic surfactants, and other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or a derivative thereof, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, etc., and their sulfates, phosphates, nitrates, citrates,
Or amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a usual manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later, but usually, water, methanol, ethanol, propyl alcohol, isopropyl alcohol, etc. Select from alcohols, lower alcohols such as butanol and isobutanol or water-containing lower alcohols, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin or water-containing polyhydric alcohols, acetone and various organic solvents such as ethyl acetate. It is desirable to use one or a mixture of two or more of these. However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after the extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Giving flexibility, relieving irritation, relieving stress by fragrance, activating cells (preventing cell aging), suppressing inflammation, improving skin and hair quality, preventing and improving rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, relief of fatigue, promotion of blood flow,
In addition to cosmetic effects such as warm bath effect, fragrance, deodorant, thickening,
Antiseptic and buffering effects can also be expected.

In foods and drinks, there are healthy and cosmetic effects such as nutritional supplement, recovery from fatigue, tonicity, cell activation (prevention of cell aging), etc., improvement of taste, provision of color tone, aroma and gloss, stabilization, It can be used for the purpose of thickening, preserving, etc. Furthermore, in addition to this, we expect various cosmetic and pharmacological effects of each raw material known so far, and aim to increase the intended effect by combining them,
Alternatively, it is possible to provide a product that is expected to have a multifunctional effect.

Specific plants (crude drugs) as raw materials include:
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsu-la-fuji (Kibo), Aotenma, Acacia, Akasho (Akasho), Akasuguri (fruit), Red clover, Red grape, Akaminoki (Logwood), Akamegawiwa (Red bud), Akaminokane or Akane Akane or Akane (Akane) Grass roots), red pheasant or geese (land yellow),
Agi (Awei), Aki larch, Akinire (Lolyu bark), Aguay Guacu, Aguahe (Wolverine palm), Akebi
(Kidori), Asa (Jin Asako), Malva Asagao or Asagao
(Toshiko), hydrangea (hydrangea), ashitaba (morning leaves), azuki (red azuki bean), asunaro, acerola, asenyaku (asenyaku), acira (shokuyoukanna), anise, abiu, abiulana, rape and abemaki `` fruit '' ,Avocado,
Ama, Amacha (Amacha), Amachaworu, Amadokoro (Jade bamboo), Amana (Kojingi), Amaranthus (Hyuyu, Hagaito, Himogeito, Senninkok, Sugimorigeito,
Hosoagaitou, Aoagetoi, Haribiyu, Aosuzuran, Amaranthus hippochondriax), Amigasa lily or Bimo (shellfish), Aristolomelia (Urizusensen), Arcana (Alcanna), Algarrobo (Cave), Altea, Arnica, Alpinia or Katsumadai
(Sorghum), aloe (lux), aloe vera, angelica, apricot or hon apricot (almond jelly), ansokko (benzoic acid), yellow sapote, scutellaria (biliary head), epimedium or primrose (inyokaku), rush
(Wicked grass), Knotweed (Tiger root), Yew (1st), Strawberry,
Figs (fructose `` fruits and leaves ''), Ichihatsu (primary), ichibi (winter aoiko), Ichiyakusou (one medicinal herb), ginkgo (ginko `` seed and leaves ''), Itohimehagi (enshi), carob, and locust bean (Telexa) , Inubeil (Honagainubiru), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou, nettle, ylang-ylang, iwa-tobacco
Iwahiba or iwamatsu (makikashiwa), oolong tea, fennel
(Fenka), Wheat (Chibusanoki), Ukiyagara (Sanryong), Uguisukagura `` fruit '', Himeukikyo, turmeric (Utsukin), Duckweed (buoying), Usubasaishin or Keirinsaishin or Oushusaishin (spicy), Usveniaoi , Foxglove, euphoria, moray eel
(Summer hay), Udo or Shishiudo (Kang-Kang, Independent, Tang-Kun), Unyadegato (Unkaria, Cat's Claw), Uba Tea, Ume (Oumei `` seed, pulp ''), Vladimir, Uwaurushi
(Kusaco peach), Unshu mandarin orange (Chen skin), Unboku,
Estragon, Ezokogi (Ezo Goka), Ezo lily of the valley,
Echinashi (Hoshibamurasakibarengiku), Enishida, Enokitake (Enotake), Ebisugusa or Cassia Tora (Keyoshiko), Elcampre, Elderberry `` fruit '', Elemi, Elingii or Pre Lotus Elinge, Ende
(Gossip, Sophora rice), ogre or Kibana-ohgi (Astragalus), Narco lily or Kagi Kurumabanaruko lily (Yoshisei), Ohhisasanoyuki or Sasanoyuki, Oren (Yellow), Ogata Hawken, Ookarasuri (Karokon), Ooguruma (Civil) , Ootsuji rafuji (self-defense), Psyllium (vegetative child, vegetative grass), psyllium thistle (Keju sashimi, bald man), eucalyptus (Haruko), pygmy cactus, pygmy pygmy or ophylla (white jitsu), pygmy pygmy (banaba), Beetle shabushi `` fruit '', giant beetle (grain sperm), beetle catechu, beetle hawthorn or hawthorn (mountain fry), barley
(Barley), oka, okazeri (snake bed), okra `` fruit '', oguruma (turnover), okururi higotai (yushu sashimi, indigo sashimi),
Panax ginseng or horseshoe ginseng (carrot), Hypericum perforatum or Hypericum perforatum or St. John's wort (brother's cut grass), Odorikosou (intermittent), Onamimi (blue ears),
Onigurumi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tianma), Oniyuri or Sasasayuri or Hakatayuri (Yuri), Onoeran, Ononis, Halibut
(Hihayu), Ominaeshi (losing sauce), Oyuco, Dutch mustard (Watercress), Dutch jelly, Dutch biyu, Dutch honeywort, olive "fruit, seed, leaf", oregano, orange "fruit, pericarp," Yellow), cacao "fruit, peel, seeds", kaki (persimmon "leaf"), kagikazura
(Hooks of Choto), Persimmon or Kantriso (Rensenso), Kakiran, Gaku hydrangea, Cassia, Kasinaki (Kozou `` fruit ''), Kajutsu (Shazajutsu), Kashiwa (Yanagi, Yuzuha), Kascarilla, Kascara sagrada , Gypsophila, gypsophila, kanikusa (kinsato), kaniwa, carnation, valerian (valerian root), hippopotamus, birch or weeping birch (white birch),
Gerbera, Pumpkin, Capoknoki `` seed '', Kahoku Sansho (shopper), Gama (bamboo), Chamomile or Roman chamomile, Kamiyatsude (weed), Kamkamu (duckling), Color, Karakakeman, Ragusuri or Shinakorasuri
(Our melon), crow squirrel (midsummer), oats, guarana `` seed '', karahoo (thick), karaya, garyu, karin
(Gourd melon), Garcinia, Kawamidori, Kawara Psycho (Koryosai, Hakubaku), Kawazusha, Kawaratake, Kawaranadeshiko (Ishitake) or Ezokawaranadeshiko (Qumugi, Quumiko), Kawaranginjin (Blue Gourd), Kawamuragi (Inchinchinko) , Kansui
(Sweet), licorice (licorice), cantaraasa, candelilla,
Canto, canna, canlan, raspberry (ezo strawberry,
Dutch strawberry, shrimp strawberry, pear strawberry, maple strawberry, European strawberry), kiwi `` fruit,
Leaves, chrysanthemum cucumber (urorone), kikyo (bellflower, bellflower root), chrysanthemum (chrysanthemum, chrysanthemum, koseki nogiku),
Kikutaniki, Catalpa (Azumitsu), Rumex (Yotei), Pheasant (Kikomi), Kizuta, Kidachi Aloe, Kidachihakka, Kina, Kinoki (Shincona, Akakinoki), Quinua (Kinoa), Kihada (Huangbashi), Gymnema sylvesta, Kimmenkaku, Cabbage, cabbage "immature fruit", caraway,
Cucumber, Gyoryu (Nishikawayanagi, Ichiyanagi), Kiraja Saponaria, Kiraya, Kiranso (Kinkan grass), Kumquat `` fruit '', Ginsekai, Kimbusen, Kinma, Kinmizukiki (Sengaku grass), Kinran, Ginran, Kinryohen,
Guapeva Vermelha, Guava "fruit", guayule, Quercus infectria (gallicate), cucuin nut, cugenumaran, wolfberry (cucumber, cucumber, cucumber leaf,
(Skin bone), Cedar grass (Tenmen Fuyu), Kudzu (Kakkon), Camphor tree, Gooseberry `` fruit '', Xining ginseng
Kutiti River, Gardenia (Gardenia), Kunugi (Bokusou), Kupuas, Kubeba, Kumazasa, Kumatsuzura (Epispermia), Clara (Bite), Cranberry "Fruit", Chestnut "Seeds, Fruits, Astringent Skin", Curculigo Latifolia `` Fruits '', grapefruit `` fruits / leaves '', buckthorn, stag beetle (needs rescue), turtle crab or kurobana chick sprouts or cypress (extended life grass), cloves (cloves, cloves), gumbinazuna (sekimei, sekimeishi),
Kaigai (Thorndust, Ikutakeho), Celosia (Rooster, Chicken),
Gekkavidin, bay (laurel), Kenashisarutibara (Tsuchukuryo, Yamakiko), gentian, gennoshoko
(Old tongue grass), Kemponashi (Kigushi), Koukisekikoku, Kishumitsukan (Koji, Tachibana, Oveni mikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana bark)), Koushinbara (Monthly flower), Kosuihakka, Kozo `` Fruit '' , Kocha (tea), Kouhone (river bone), Kohon
(Straw book, Kara straw book), Koryang, Kouryokyo (Kora Kyo), Cilantro `` fruit '', Kourouen (Kohren), Koganebana (yellow gon), Cowberry (Koshibachi), Coco palm `` Fruit '',
Goshuyu (goshuyu), Goshoichigo (covered bonko), pepper
(Pepper), cosmos, copaiba balsam, coffee `` seed, leaves '', kobushi or magnolia (hot spring), burdock (burdock,
Burdock), kobotdur, sesame (sesame), sesame seeds (gensin), garnish (gomiko), sanekazura or binankazura or matsubusa, wheat (wheat), rice or rice bran "red bran, white bran", rice oil, cola acuminata " Seeds ", cola
Vera “seed”, colony lya, fenugreek “fruit”, colombo, conzlango, kelp, konjac, comfrey
(Final barn grass), sisal (sisal asa), saihairan, sakaneran (ezosakaneran), saxima button or syna button or cinnamon (weird sen), cherry
(Ooshimazakura, Yamazakura, Oyamazakura, Edhigashi, Mamezakura, Miyamazakura, Someiyoshino, Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura `` Leaf, flower, fruit, bark (cherry bark) ''), cherry, pomegranate, sasa, sasabagin , Sage Modaka (Sawaka), Sweet Potato, Sugarcane, Sugar Beet, Sanebu Jujube (Satsuma Natsume), Saffron (Safflower, West Safflower), Sapodilla, Sapotea Marillo, Pomelo "Fruit", Sabonsou, Salmonberry "Fruit", Sarasin
(Masahima), Saranshito (Sand Ai Sashimi), Salvia (Sage),
Sawagikyo (Yamakona), Sawagurumi (Yamawomo), Sankaku cactus, Sangre degrad (Croton), Sansouyousou, Sanshin ginseng (Sanjin ginseng), Sanshuyu (sanshuyu), Sanshoyu (sansho), Sunzucon (Yamato) Root), shea
(Karite), Cyano (fruit), Shiitake (Shiitake), Sion
(Shiwon), Red-spotted squirrel, Shikunshi (Kimiko), Perilla or Aozo or Chirimenji or Kamenjiso (Shiyo-leaf, Shiso-so), Rosewood, Shinanoki, Shinanohonoki, Chinese forsythia (reamous forsythia), Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshirohimeji) Bunashimeji, honjimeshi, shirotamogitake), shimokuren (hot spring), shimotsukesou, potato, peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (wheat gate winter), shukukonemisou, shukushamitsu (sunin, Sand), Juzudama,
Palm `` fruit '', Shunran, ginger (ginger), Japanese hydrangea (Tsuneyama), shobu (iris, iris root), shizuku `` fruit '', shirakashi `` seed '', silane (byakyuu), shirogoshi `` seed '', white clover (clover) ), White adzuki bean (chicken bone), White bean iris (Small iris), White bean ivy “flower”, White bean (Nami Tenmi), Cinchona sassilbura, Jinjuge (Mizuka, Mizuka, Shencho flower), Cinnamon, Cinnammum cassia , Watermelon (watermelon), honeysuckle (gold and silver flowers, Shinobi winter),
Sorrel (acid model), swim berry (fruit), star apple, stevia, strawberry (fruit), suzu psycho (Xu Chang Lord), horsetail (Qingi), purslane (horse dent, horse dent), plum berry, sulgaran (Oran), Pinus densiflora `` cone '', larch, vegetation, walnuts, hawthorn, eucalypt, dandelion, horse chestnut (malonie),
Pear `` fruits '', snowy pear, elderflower, elderflower, juniper (juniper, juniper), yarrow (milfoil), rose, foliage, mistletoe, mint, or swordfish (Ishishone), Sekko (Sakura Sekko,
Red-crowned, Red-crowned, Red-crowned, Red-crowned, Red-crowned, Red-crowned, Red-crowned (Dendrobium, Stone), Cedron (Bowshuku-boku), Zeniaoi, Hirohase Nega, Senega, Seri, Sinensen, Sinensen, Sinensen Sengdan, Sennin cactus, Senna `` fruits and leaves '', Senninsou (large tate), Assemblage (drug),
Sengoku, souka (grass), elephant, sosinoubai, buckwheat `` seed '', soybean potato, rhubarb (daisuki), radish, soybean, daidai (orange, kikomi), takasagorurihigotai (southeast indigo), takasaburo (dry lotus) Grass), Takatodai
(Daigei), Takawarabi (Inu), Dark Sweet Cherry `` Fruit '', Tachiaoi, Tachijakosou (Thyme, Hyakuka), Tachidokoro (Hikai), Tachiyanagi, Tamarind `` seed '', Onion, Tamashiba (Shinyi), Taranoki `` Fruit '' , Leaves, root bark '', tarayou (large leaf winter blue, one leaf tea, bitter tea, bitter tea), dahlia (tenjikubotan), tarwi, tanshiuchiwa, tanjin (dansan), dandelion (gakkoei) or white dandelion or moko dandelion, Dammar, Cherry `` fruit '', Tigaya `` fruit, root, bud '', chix carrot (bamboo ginseng), chicory, chancapiedra (Kidachikomikansou), tulip, chocolate nose, Korean blossom (Ohio), Korean cinnamon (Bouyi) ), Artemisia (Aiba), Choreimaitake (Irei), Chorogi, Tsukimiso, Tsukushikaneran, Tsukuritake ( Mushrooms), Tsuchiakebi, Camellia, Camellia, Tsumekusa (lacquer lacustrine), Tsujukusa (Duckling grass), Tsuruazuki (red azuki bean), Tsurutsuchiakebi, Tsurudukudami (Hanshu crow), Tsuruna (Ban apricot), Tsuruninjin (four leaf ginseng), Tsuwabuki, Deiko, Teuchigurumi,
Duberry `` fruit '', tengusa, tencha (tencha), tendai yak (crowd drug), touga (winter melon), toukaizuka,
Capsicum (Pepper), Touki (Toki), Calendula (Marigold), Tonabena (River cut), Maize or Maize hair (Nanban hair), Toad Mochi (Female Sadako), Toudodou or Korean gentian (Ryubile), Tokusa (wood pirates), dokudami (jujutsu), konkon (sore root), toshishi or mamedaoshi or pear, and eucommia (tochu `` bark, leaves,
Roots), ash (qin peel), tomato, tragacanth, triacanthus `` seed '', eustoma, tormentilla, dronoki, troloioi, tongue bean, Niseria berry `` fruit '', potato or yam (herbal medicine), squid
(Butcher Bloom), Naginata Kouju, Nazuna, Rape, Natsume bean or Hatehaki (sword bean), Natsumikan, Jujube (daijutsu), Naniwaibara (Kanakurako), Navena (intermittent), Nameko, Peanut (peanut), Nanten (Nanten) Fruit), Nanban karamushi (ramie), scented violet, nigaki (bitter tree), bitter mugwort (bitter), nutmeg, kei or nikkei or ceylon nikkei or yab nikkei (cinnamon bark) or keishi (keishi),
Grey-eared plover, Chinese chive (Nirako), elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nulde (quintuple), leek, nemuki or nebu or nebi or nemlinoki or zigokubana (health), noami ( Daiji), Neubara (real fruit), Nori Kazura (Liao Xiaohua), Nogateto, Yarrow, Nodake (Maehu), Nobara, Nomomo, Pineapple `` Fruit '', Hibiscus (Bussouge, Furinbusouge, Roselle), Hyindou, Hauchiwa, Pau Dosse , Hakama Uraboshi (Bone Crushing), Hakuka Yumato, Hakusan Plover, Hakusen (Ringworm Skin), Huckleberry "Fruit", Hakobe (Horuko), Hashibami (Haruko), Hashiridokoro
(Roth root), basil, lotus (lotus, lotus, lotus), parsley
(Netherland jelly), naked mussel, batata, bee or medake (bamboo boiled), patchouli, mint (lightly loaded, lightly loaded leaves), barley (yokuinin), hanasuge (chimbo), banana, hana mint, hanabishi (situri, shisuturi), vanilla bins , Papaya, mudweed (rat malt), pahalobobo, hub `` whole plant, stem, leaf '', paprika, hamakakiran, hamagou or honey beetle
(Ritsuko Riko), Hamanas (maikai flower), Hamabofu (beach storm), Hamelis, palm, rose (rose), balata,
Balata rubber tree, agaric mushroom (aratake mushroom, white agaric mushroom, usukimori no kasa), haran, pariurus (bamboo jujube), parietalia, harkanran, balsamina (surreale, bitter gourd), harunire (yu bark, yu white bark, yuba), barbasco, harindou Handaikai
(Giant Sea), Breadfruit, Hiogi (Ranker), Lycopodium ginseng (Party), Pecan Nut, Higanbana (Shirubi, Jinjusha Hana), Higuru Madaria, Higotai, Hishi (Hishimi), Pistachio, Beet, Hitotsuba (Stone) Reeds), Aedes fern,
Daisies (daisy), hinata nokozuchi (cow knees), hinio bulbul, hinoki, hiba, castor, sunflower, bell pepper,
Himeuzu (Tamaoi), Himegama (incense bud), Himematsutake (Kawariharatake, Hiromatsutake), Himemyouran, Pimenta `` fruit '', juniper, beakyu, hiyu `` fruit '',
Biloba oi, Hiroha okinagusa (Hakuto Okina), loquat "fruits, leaves, stems", betel nut (large clothes skin, betel nut), puerh tea (pu-cha), scallop (southern rattan), squirrel, serrata Winter leaves), fujibakama (orchid grass), faba bean (tonboshi), fujimokiki (choujizakura, shinbana), grape "fruit, peel, seeds, leaves", beech, beetle
(Cordyceps), Brazilian Licorice, Brazilian Carrot, Black Currant `` Fruit '', Blackberry, Bragnien, Plum `` Fruit '', Flucellaria, Blueberry
(Prunus avium), prunes, floral blanka, blonde psyllium, bundo (green beans), hazelnuts, loofah, hetsukaran, safflower (safflower), henneken, belladonna, berry `` fruits '', percea, perbianberg, pereschia grandifolia, benceisoa Or duckweed (Keiten), boysenberry "fruit", duckweed or elderweed or nendowe or hawakigi kokia
(Skin), Hosairan, Balsam (Hosen, acute child,
Translucent grass), Potteria sapota, Potteria lukuma,
Spinach, boufu (windbreak), spinach, physalis
(Torone), Honoki (Japanese thick and magnolia), Bokeh (Gourd), Hosoba Aburagiku (Biyoku), Hosobaoguruma, Hosovanaokera
(Soujutsu), Japanese beetle (Shinjuku Michiten), Japanese beetle, Bodaiju (bodice tree), button (peony `` flower, leaf,
Stalks, bark ''), buttonpi (peony bark), hops, jojoba, borage (ruriticsha), bordeaux, white berries `` fruits '', hon-oniku (meat jiyo, daitei), hon-sekkoku (iron bark stone, sparrow mountain stone) , Maizulten Nanshaw (Tennan Star), Maitake (Maitake), Ephedra (Masochism), Maca, Macadamia Nuts, Margaret (Mokshungiku), Macri (Seaweed), Magwa (Mulberry White Bark, Bark, Leaves), Magnolia・ Splengeri, Masaranpouba, Masaranzuba de Ceara, Masheira de Boi, Masahua (Tamanousenhallen, Kyukonkinrenka), Matatabi (Kiten Tate), Matsukasa, Pine `` leaves, bark, roots '', Matta Oryo, Matsuhod (Bukuryo), Matico (Cordoncillo), Mayorum (Hanamint), Malvanozadin (Bittersweet), Mulberry "Fruit", Quince, Malein (Below Mow) Watermelon), Mango, Mangosteen, Mansagna (Andesca Mitsure), Mansugurumi, Mandarin `` fruit '',
Mannentake (Lingzhi), Pheasant (Kikomi `` fruits ''), Mishimasaiko (Saiko), Mizuobako or Mizuasagao (Ryugoshi),
Mizokukushi (Hanbe Lotus), Misohagi (Chisatsuna), Mizunyanagi or Shiroyanagi (Henryoku), Mitsugashiwa, Mitsuba, Midorisapote, Midorihakka, Mimosa, Myoga, Miracle Fruit `` Fruit '', Myrrh, Milobalan, Wheatwaragiku, Mukuge (Mokuge) , Mukunoki, Mukuroji (extended skin), Munya, purple (purple root), purple or purple purple
(Purple beads), purple corn, purple purple (Kunming chicken blood wisteria), megakanza opuntica, red seaweed (mother grass), red seaweed, melaleuca, melissa, melilot,
Melon `` fruit '', sagebrush, moso bamboo, moku giraffe, moje (koshoboku), ilex, mokko (wood incense), maple blossom, peach (peach `` leaves, seeds, flowers, fruits ''), bean sprouts, morello cherry , Moroheiya
(Burlap), yakawamurasakiimo, yakuchi (masuti), cornflower mackerel (cornflower), cornflower mint, yacon, yashabushi (arrow car) or himeyashabushi or giant yashabushi `` fruit, peel, kaho '', yachiyanagi, yatsude (octagonal metal) , Mistletoe (willow parasitism), willow (river willow, twill willow, weeping willow, red willow, pussy willow, dog puppy willow, kinuyanagi, kolyanagi, unryuyanagi, yamayanagi, palm, yamayanagi, obanyanagi,
(European willow, fox, willow tree)
Japanese tobacco (Tsurusui, Tenmei), Japanese pokeweed (land), Japanese alder (Yamaharu), Japanese peach (Yumemei), Japanese sagebrush, Eucalyptus, Yukinoshita (Tiger ear grass), Yucca or flevifolia, Yuzu `` fruit '', lily, Artemisia, mugwort (Aiba),
Rigan (Raimaru), lime `` fruit '', rye, lacanca `` fruit '', raspberry `` leaves, berries '', ratania (clameria), raccoon or shallot (scallop), lavender, longan (longan meat), agave (Aonouragetsu, fukurin) Agave), Ryokucha (green tea), Apple `` Fruits, seeds, leaves, roots '', Gentian, Rubus or Suabisimus (Tenryu), Ruritama thistle (Urajirohigotai, Xinjiang indigo sashimi), Reishi (Lichi, Lichi nucleus), Lettuce (Chisha), Red currant `` Fruit '', Red pitaya, Lemon `` Fruit '', Lemongrass, Forsythia or Chinese forsythia (forsythia), Astragalus, Lumbai (wax plum), Waxy palm, Lokon (Yoshi, Azuki), Loganberry `` Fruit '' , Rosemary (Mannenrou), Rosehip (Nobara), Wasabi, Cottonweed Remokou
(Chiyu) and the like.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidsa, Chlorella ellipsoidia, Aonori (Usubaonori, Susiaonori, Hiraaonori, Bowaoonori, Hosoedaoonori), Anaaousa (Aosaa) and the seaweed [Aosa] Kinds: Kombu (Macambu, Rishikonbu, Hosomekombu, Mitsuikonbu), Wakame, Hirome, Ao-Wakame, Giant kelp (Macrostistis pilifera, Macrocystis integrifolia, Neocitys luetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Klebanoumi prickly pear, Akabumi prickly pear, Konau prickly pear, Okina prickly pear, Usuki prickly pear, Echinoki prickly pear], seaweed [red algae: Hijirimen, maxa (Tengusa), hirakusa, oniku , Obakusa, Kataobakusa,
Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Junyashinkirinsai, Tsunomata, Obatsunomata, Tochaka (Yahatsutsunomata), Ezotsunomata, Togetsunomata, Hirakotonota, Kotojita
Jareus Banori, Kagius Banori, Siusius Banori, Hyus Banori, Akamomininori] and the like.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tulsimidro, Hibimidro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asiaticum gutta, kawashiogusa, marimo, baronia: tamagobaronia, tamabaronia, tamagata: tamagatamo, iwata: husaiwata, surichogita, heraiwata, kurokita, kurokita, kurokita Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizudzumo, Genus Genus, Genus Colecaetus, Genus genus Tsutsumo, Artemisia spp .: Artemisia spp.

Blue-green algae (Genus Lynx: L., L., L., L .;
(Hassai, Yuremo, Racenmo (Spirulina) genus: Spirulina, Trichodesmium (Aiakashio), etc.).

Brown algae (Piraella spp .: Piraella spp., Spirogyra spp.
Genus Isogawara: Isogawara, Kuroshira Genus: Gunsenkurogashira, Kashirazaki Genus: Kashirazaki, Muchimo Genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa Genus: Amidigsa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common Genus Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawazuku, genus phlebosum, genus phlebosum, genus phlebosum: genus futomozuku, genus genus moss: genus genus: genus kuromo, genus moss, genus genus Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Betula serrata, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakuganusa: Shakuganusa,
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Genus: Genus Genus: Genus Filiformis, Genus Turmo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Musicum, Genus Missiscoma, Genus L. , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombu genus: Kikuishikombu, Nejirekombu: Nekorecome genus, Kuroshimae : Nekoshikambu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-moku, Obanoki-mimoku, Genus genus:
Bulukimo, genus cynomolgus: cynomolgus etc.).

Red algae (Russia genus: Ushikenori, Funorinoushike, Amanori: Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acer genus, Acer genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: rattle, rattle rattle, hirohatamatamaki genus: hirohatamaitadaki, tamamaidaki genus: tamaitadaki, kagikenori genus: kaginori, kakenenori, tengusa: himetenugosa, haitenugosa, haitenugosa, haitenugusaugou, haitenuganugou, haitenuganugou, haitenugusaugou Genus: hibiroudo, himehibiroudo,
Genus Isommedogi: Genus Isomomedo, Genus genus: Genus medusa, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekususa: Genus Nisekaregusa, Okitsubara: Obaokitsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattle, mushroom, shiogosagoromo, iwanokawa: ezukiiwanokawa, kainokawa: kainokawa, kaninote: kaninote, coral genus: coral, coral, mosquito mosquito , Tambanori, slippery,
Fudarak, Malbahudarak, Isonohana: Nuracusa,
Cranouraxa, Stinging skull, Hunting genus: Hunting ibis, Hunting genus: Hunting sword, Hunting genus: Chabo kintoki, Hunting ibis, Matsunori, Komenori, Tosakamatsu, Aedes pine, Fushikintoki, Tsunomukade, Nagakinotoki, Scutellariae, Osmanthus Nagaobabane: Nagaobabane,
Genus Funori: Hanafunori, Fukurofunori, Makunori, Kalexigusa: Kalexigusa, Sasamodoki: Hosobanotosakamaki, Hirohanokasakamuki, Yatsudegatatasakamoki, Kurosakamaki, Nezashinosakamukado, Yukasakanookado Modoki,
Genus genus, genus genus: quinohada, genus esculenta: esoteka, tsukasanori genus: enashikarimenia, ootsukasanori, hanagatakarimenija, honooo genus: honoo, genus genus: lyca genus, genus genus, genus nikonobenigo Susqueke Beni, Okamuragusa:
Yamadakusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiibanodan, Kijinotho genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurusilamo, Shiramo, Ogogonori, Mizookonori, Kabanori, Beniogonori, Fucresinori, Murasakikabanori, Shinkaikabanori, Togekabanori, Kataogonori, Ryukyuogonori, Ryokyuogonori, Ryokyuogonori, Ryokyuogonori, Ryokyuogonori , Genus Amanita: Hachijoenaga, Fushikenori genus: Fushikunori, Namiiwatake genus: Namiiwatake, scorpion genus: Scorpion genus, Occidental genus: Okutsunori, Saimi genus: Itanisa, Saimi, Harigane, Lotus japonicus:
Lotus spiders, genus genus: Ikanoashi, Hosoibonori,
Nobonori, Black Snapper: Black Snapper, Red Snapper: Red Snapper, Hishbukuro: Hishbukuro, Madaragusa: Togemadara, Echimadara, Taoyagousou: Genus Ottawa, Scrophularia , Hirata goat: Hirata goat, Dulce: Dars, Masagoshibari, Anadalus, Uebagsa: Uebagsa, Benifukuronori: Benifukuronori, Fushinagi: Fushitsunagi, Himefushinagi,
Species of the genus Hirohafushi, Snapdragons: Snapdragons, Snapdragons, Species: igis, keiigis, crickets, nigiri, amixa, egonori:
Egonori, Futogigis, Saeda: Saeda, Chrysanthemum Genus: Chrysanthemum, Konohanori Family: Habutaenori, Konohanori, Suzushironori, Usbeni: Usveni, Hasuginu: Hasuginu, Nagakonohanori: Nagakosugino, Genus: suginanu Yareusbanori, Sujiusbanori, Hyusbanori, Usubanorimodoki: Usubanorimodoki, Ayanishiki: Ayanishiki, Ayaginu:
Ayaginu, Dassia genus: Enashidazia, Simadadia genus: Isohagi, Shimadada, Dazimodoki genus: Daziomomodoki, Itogusa genus: Moletogusa, Futoigusa, Macri genus: Macri, Yanaginori genus: Hanayanagi, Yuna, Sanaginori, Mosanyugina, Sasa, Rabbitum : Kurozozo, Kobusoso, Hanezozo, Sozonohana, Hanegusa: Hanegusa, Kanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosokozanemo, Himegoge: Himegoke, Kurohimegoke, Hiodoshig, Hoshigusa Suginasugusa, Isobasho Genus: Isobasho, Fujimatsumo Genus: Fujimatsumo, Sawwort: Genus Hakezakinokawa, Kawamozuku: Kawamozuku, A Kawamozuku, Himekawamozuku,
Ideyugogome: Ideyugogome, Okitimozuku: Okitimozuku, Itogusa, Chinorimo: Chinorimo, Chisginori: Chisinori, etc.).

Axles of the axle (Charium spp.
Hossiturimo: Hossiturimo, Lichnotumnus, Flasks: Himegrass, Chabograss, Tripera etc., Yellow algae (Hikarimo: Hikarimo etc.) and the like.

Materials derived from animal raw materials include cockscomb extract, bovine or swine, human placenta extract, bovine or swine stomach, duodenum, intestine, spleen extract or its degradation product, bovine or swine. Extract of brain tissue, collagen water (acid, alkali,
Enzymes, etc.) Decomposed products, collagen derivatives such as water-soluble collagen or acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its decomposed products or their derivatives, silk proteins and their decomposition Products or their derivatives, swine or bovine hemocyte proteolysates (globin peptide), bovine or swine hemoglobin hydrolysates (hemin, hematin, heme, protohem,
Heme iron), milk, casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg components, fish meat decomposition products, nucleic acid-related substances (ribonucleic acid, deoxyribonucleic acid) )
etc.

(14) Marine components Seawater such as deep water, for example, seawater salts, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud Or mud (Fango) s, for example, sea mud or mud (constituents: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, iron oxide, manganese oxide, sodium oxide, etc.) such as Italian fango, German fango, Eifelfango, Freiburgfango, etc. Potassium oxide, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintoku stone, etc.

(15) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice fermented extract, rice bran (red bran, white bran) fermented extract, Euglena extract or its decomposition product or These water-soluble derivatives, lactic acid fermented products of raw milk or skim milk powder, trehalose or derivatives thereof.

(16) α-hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(17) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(18) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonic acid salt, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid Derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate,
Octyl paramethoxycinnamate, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, mono-2-diparamethoxycinnamate
Glyceryl ethylhexanoate, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate,
Methyl salicylate), anthranilic acid derivatives (methyl anthranilate), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B6 derivative, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, slisobenzone, benzoresorcinol, arbutin Guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin and the like.

(19) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and the like.

(20) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate ester magnesium salt, vitamin C glucoside or the like), hydroquinone or a derivative thereof (hydroquinone benzyl ether or the like), kojic acid or a derivative thereof, vitamin E or Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (chamomile, mulberry, gardenia, touki, waremokou,
Clara, mugwort, honeysuckle, yellowfin, amberjack, matsuhodo, adlay barley, scotch, hops, hawthorn, eucalyptus, achillea millefolium, artea, calyx, mankeishi, hamamelis, yamaguwa, extended life grass, bellflower, toshishi, maizeko, sun dried, mao, Sengoku, Dokkatsu, Psycho, Bohu, Hamabofu, Ogon, Peony, Peony, Gennoshoko, Kakkon, Licorice, Gotobiko, Aloe, Shouma, Safflower, Green Tea, Black Tea, Asenyaku).

(21) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether)
etc.

(22) Turnover promoting action / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, Horsetail, Gentian, Burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odrich, Assemble, Touki, Calendula officinalis, Amacha, Hypericum perforatum, Cucumber, Periwinkle,
Rosemary, parsley) etc.

(23) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compounds) etc.

(24) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(25) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaseletenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emisoso, Birch, Salvia, Rosemary, Minami Tenji, Agetsu, Ginkgo, Green Tea) and the like.

(27) Anti-inflammatory agent: ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract, propolis and the like.

(28) Antibacterial, bactericidal and disinfecting agents acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, chloro Butanol, isopropylmethylphenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene Octylphenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde , Hexamine, brilliant green, malachite green,
Crystal Violet, Jamal, Photosensitizer 101
No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin, propolis and the like.

(29) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or its derivative or chitosan derivative, pyrrolidone carboxylic acid or its Salt, Sodium Lactate, Mini Sasanishiki Extract, Bacillus Natto Metabolite, Natto Extract, Loofah Water, White Birch or Akamatsu Sap

(30) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, salvia, elderberry, bollige, buttonpi), seaweed extracts and the like.

(31) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(32) Antiandrogens Estrogen (estrone, estradiol, ethinylestradiol, etc.), isoflavones, oxendrones and the like.

(33) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(34) Stimulants Pepper tincture, vanillamide nonylate, tincture tincture, tincture tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(35) Metabolic activators Photosensitizer No. 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placenta extract, biotin, glyceride pentadecanoate and the like.

(36) Antiseborrheic agent pyridoxine or a derivative thereof, sulfur, vitamin B6
etc.

(37) Keratolytic agent Resorcin, salicylic acid, lactic acid, urea and the like.

(38) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium silicate, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution,
Mushroom extract and the like.

(39) Hair remover strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide, thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like;

(40) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(41) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol ,
2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, ortho Aminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-
Diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol,
Picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenyl Paraphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate,
Orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-sulfate
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy
Sodium 5-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(42) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Greater Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemon grass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(43) Dyes and Colorants Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikasumi Dye, Turmeric Dye, Endu Dye, Krill Dye, Persimmon Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin and the like.

(44) Others Hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, fresheners, stabilizers, emulsifiers,
Vegetable proteins and their degradation products, animal and vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound healing agents, There are foaming agents, thickeners, enzymes, purified water (electronic water, small clusters, etc.), oral agents, deodorants / deodorants, etc., and when used in combination therewith, the effects of the present invention or other cosmetics It can be expected that additive or synergistic improvement of the objective effect will be achieved.

The moisturizing agent, skin roughness improving agent, hair protecting agent and cosmetic composition of the present invention are respectively liquid, emulsion, base, gel, powder (powder), granule, pellet, and stick. , Solid form and the like.

Specifically, for example, external preparations, basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers and skin cleansers, depilatory agents, depilatory agents, aftershave lotions , Pre-show lotion, shaving cream, foundation, lipstick, blusher, eye shadow, eyeliner, makeup cosmetics such as mascara, perfume, nail polish, nail enamel,
Beauty nail enamel remover, shampoo, rinse, hair treatment, pre-hair treatment, hair styling, perm, hair tonic, hair dye, hair growth / hair restoration, hair cosmetics, bath agent, deodorant, deodorant, pup Preparations, plasters, tapes, sheets, patches, aerosols, and other products such as toothpastes, mouth fresheners / gargles, sanitary cotton, wet tissues, etc. Can also be used for

The method of adding the product of the present invention to a moisturizing agent, a roughening agent, a hair protecting agent and a cosmetic composition is described below.
It may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

[0069]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

[0070]

EXAMPLES Production Example 1 of Collagen 40 kg of frozen skin of sole (sole) was crushed, and _two pieces of phosphate buffer solution (21.7 g / L Na ₂ HPO ₄ and 0.78 g / L KH ₂ PO ₄ ) were obtained. Washing was performed in a continuous tank. The concentration of the milled material was 1 kg for a 5 L tank and the contact time was 1 hour. The phosphate was then removed by centrifugation using a continuous centrifuge rotating at 4000 revolutions per minute, and two successive washes with deionized and sterile water. Next, the obtained residue was added to a 10 L tank at a concentration of
Extracted in 25M acetic acid solution. The mixture was stirred for 15 minutes and the supernatant was collected by continuous centrifugation under the same conditions as above. This extraction operation was repeated twice, and the supernatant was collected. Next, a 7% aqueous sodium chloride solution was added,
Collagen was precipitated. The resulting precipitate was dialyzed with deionized water until the salts were completely removed. The obtained collagen may be placed again in the acidic solution or lyophilized. In this case, 1.6 kg of dried collagen was obtained from 40 kg of fresh skin of sole. The extraction yield was more than three times the amount obtained for calf skin.

[0071]

EXAMPLE 2 Collagen Production Example 2 40 kg of frozen abdominal skin of frozen sole (sole) was crushed and washed with a phosphate buffer and water in the same manner as in Production Example 1. Next, 4 kg of 4% sodium carbonate was mixed with 1 kg of the crushed material at a ratio of 4 kg, and the mixture was stirred by a stirrer until the mixture became uniform, and then left at about 15 ° C. for 5 days. Next, the pH was adjusted to 5.5 to 6.5 with an 80% acetic acid solution, and then the pH was adjusted to 3.0 to 3.5 with a 12N hydrochloric acid solution. The neutralized ground material was decanted with a Russle decanter and the supernatant was recovered. The resulting mixture was poured into a dialysis membrane and dialyzed against deionized water for 10 days. The dialyzed product was diluted with a 0.1% sorbic acid solution while stirring to adjust the solid content to 1.0 to 2.0%. First, 20
Filtered through a 0 micron then a 75 micron Cuno filter. This will give you 40kg of fresh sole (so
280 kg of collagen / 0.1% sorbic acid solution was obtained from the skin of le).

[0072]

[Examples] Safety test (1) Primary skin irritation test An aqueous solution (solid content: 5.0%) in which each collagen obtained in Production Examples 1 and 2 was dissolved was adjusted, and the back of the Hartley guinea pig (1) was depilated. 5 groups, weight around 320 g). The evaluation was performed 24 hours after application by using primary irritancy scoring method with erythema and edema as indices. As a result, in all animals, no erythema and edema were observed, and the animals were determined to be negative.

(2) Skin Cumulative Irritation Test An aqueous solution (solid content: 5.0%) in which each collagen obtained in Production Examples 1 and 2 was dissolved, and a Hartley-type guinea pig (female, group 1) whose flank was dehaired was prepared. (5 animals, body weight of about 320 g) were applied to the skin once a day at a frequency of 0.5 mL / animal five times a week. Application was performed over 4 weeks and hair removal was performed on the last application day of each week. Judgment was made on the day following the last day of each week by the primary irritation scoring method using erythema and edema as indices. As a result, in all the animals, no erythema and edema were observed for 1 to 4 weeks after application, and the animals were determined to be negative.

[0074]

EXAMPLES <Test 1> Moisturizing Test In order to evaluate the moisturizing property of the collagen of the present invention, the change over time of the water content of the stratum corneum by a monitor was determined by measuring the electric conductivity. (Sample solution) The collagen obtained in Production Examples 1 and 2 and purified water as a blank, and bovine-derived collagen prepared in the same manner as a comparative example were used. (Measuring instrument) IMPEDANCE METER SKICON-200; manufactured by IBS Co., Ltd. (Monitor) 24-year-old female (Test environment) 24 ° C, 50% RH (Method) A constant temperature and humidity chamber adjusted to the test environment one hour before the test Allow the monitor to enter and stabilize. afterwards,
Apply each sample solution (1 mL) to the forearm flexion side of the monitor,
After seconds, excess water was removed and the electrical conductivity of the stratum corneum was measured over time. The results are shown in FIG.

As shown in FIG. 1, it was found that the collagen according to the present invention had a higher moisturizing effect and was maintained for a longer time than bovine collagen.

[0076]

Example <Test 2> Skin roughness improvement effect test In order to evaluate the improvement effect of the collagen of the present invention on skin roughness, a guinea pig prepared with a skin roughness model was used.
An application test was performed. (Sample solution) The collagen obtained in Production Examples 1 and 2 was adjusted to a 1.0% aqueous solution. Purified water was used as a blank, and bovine collagen was similarly prepared as a comparative example. (Method) To a Hartley-type guinea pig (female, 5 weeks of age, 3 animals per group) with depilated back, sodium lauryl sulfate (0.2 g) adjusted to 3% by weight with white petrolatum was continuously and continuously applied for 3 days. Created rough skin. The rough skin preparation site was divided into four minutes, each sample (1.0 mL) was applied three times / day, and three days later, the degree of rough skin was evaluated according to a criterion (score) determined by observation. The results are shown in Table 1. (Evaluation criteria) Erythema and desquamation are hardly seen 1 point Mild desquamation without erythema 2 points Moderate desquamation without erythema 3 points Desquamation with weak erythema 4 points Desquamation with moderate erythema 5 6 points with desquamation with marked erythema

[0077]

As shown in Table 1, the collagen of the present invention was confirmed to be effective in improving skin roughness.

[0079]

Example 3 Test 3 Measurement of Slipperiness and Smoothness of Hair (Sample) The collagen obtained in Production Examples 1 and 2 was used, purified water was used as a blank, and bovine collagen was used as a comparative example. (Method) Hair was immersed in a sample adjusted to a 5% aqueous solution for 1 hour, and then washed with water. After sufficient drying, a double-sided tape was attached to both ends of the slide glass, and 15 hairs were placed so that the cuticle became constant, and then bonded using a cured epoxy resin (manufactured by Konishi). The average friction coefficient (MIU) and the degree of variation of the average friction coefficient (MMD) of these hairs were measured using a friction tester (KES-SE Kato Tech).
The measurement was performed in a room at a room temperature of 23.0 ° C. and a humidity of 55.0%. The results are shown in FIG.

As shown in FIG. 2, the degree of variation of the average friction coefficient (MMD) was lower in the bovine collagen than in the blank, but the average friction coefficient (MIU) was higher. On the other hand, the collagen of the present invention showed low values for both the average friction coefficient (MIU) and the degree of variation (MMD) of the average friction coefficient. Therefore,
The product of the present invention was found to have the property of giving the hair slipperiness and smoothness.

[0081]

【Example】

<Test 4> Measurement of permeability to hair (Sample) The collagen obtained in Production Examples 1 and 2 was used, and purified water was used as a blank. Bovine collagen was used as a comparative example. (Method) Hair was immersed in a sample adjusted to a 5% aqueous solution for 1 hour, and then washed with water. The hair was treated with a ninhydrin solution, sliced, and observed with an optical microscope (200 ×).
The results are shown in FIGS.

While the hair of the blank shown in FIG. 3 was hardly stained, in FIGS. 4 and 5 of the product of the present invention, staining was observed around the hair. This is considered to be due to the fact that the peptide in the product of the present invention, which has permeated the hair, has been colored by ninhydrin. The comparative example shown in FIG. 6 was dyed to the same degree as the product of the present invention. As a result, it was confirmed that the collagen of the product of the present invention penetrated into hair to the same extent as bovine collagen.

[0083]

EXAMPLES Preparation of Various Cosmetic Compositions Various cosmetic compositions according to the present invention were prepared. The formulation examples are shown below, but the invention is not limited thereto.

(Formulation Example 1) Emulsion weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Soorbitan monooleate 2.0 7.1,3-butylene glycol 1.0 8. Glycerin 2.0 9. Collagen of Preparation Example 5.0 10. Preservative (paraoxybenzoate) qs 11. Purified water 100

(Formulation Example 2) Emulsion Weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Glyceryl paraaminobenzoate 1.0 4. Ethyl urocanate 1.0 5. Magnesium salt of vitamin C phosphate ester 1.0 6. Cetyl alcohol 1.5 7. Glycerin monostearate 2.0 8. Polyoxyethylene (20) cetyl ether 3.0 9. Polyoxyethylene (20) soorbitan monooleate 2.0 10.1,3-butylene glycol 1.0 11. Glycerin 2.0 12. Collagen of Production Example 1 3.0 13 .Preservative (paraoxybenzoate) qs 14. Residual with purified water 100

(Formulation Example 3) Cosmetic oil weight% 1. Liquid paraffin 30.0 2. Squalane 20.0 3. Olive oil 20.0 4. Isopropyl palmitate 10.0 5. Crepefruit hot water extract 1.0 6. Collagen of Production Example 1 3.0 7 Olive oil 1.0 8. Shea butter 1.0 9. Butyl hydroxyanisole 0.1 10. Vitamin E derivative 0.1 11. Preservative (benzalkonium chloride) proper amount 12. Fragrance (musk water) proper amount 13. Purified water 100 residue

(Formulation Example 4) Flexible lotion% by weight 1. Glycerin 5.0 2.1,3-butylene glycol 5.0 3. Polyoxyethylene sorbitan monolaurate (20E.O) 1.0 4. Ethanol 15.0 5. Collagen of Production Example 2 1.0 6. Grapefruit 50% ethanol extract 1.0 7. Kiwi 50% ethanol extract 1.0 8. Appropriate amount of antibacterial and preservative (lactoferrin solution) 9. Appropriate amount of fragrance (Western thin water) 10. Purified water 100

(Formulation Example 5) Astringent lotion weight% 1. Sorvitol 2.0 2. POE (20) oleyl alcohol 1.0 3. Ethanol 15.0 4. Collagen 2.0 of Production Example 1. 5. Gentian 50% ethanol extract 1.0 6. Sage 50% ethanol extract 1.0 7. Allantoin 0.2 8. Astringent (zinc sulfocarbonate) 0.2 9. Antibacterial and preservative (thiamine lauryl sulfate) qs 10. perfume (lavender water) qs 11. Purified water 100

(Formulation Example 6) Two-layer type lotion (liquid to liquid type) weight% 1. squalane 8.0 2. POE sorbitan tetraoleate 0.3 3. sorbit 1.0 4. ethanol 15.0 5. collagen of Production Example 2 2.0 6. Xenopus 30% ethanol extract 2.0 7. Peony 50% ethanol extract 1.0 8. Peanut 50% ethanol extract 1.0 9. Ascorbic acid 0.2 10. Astringent (calamine) 0.1 11. Preservative (phenoxyethanol) qs 12 .Perfume (lemon water) Appropriate amount 13. Residual water with 100 purified water

(Formulation Example 7) Carmine lotion (liquid to solid) wt% 1. Glycerin 3.0 2.1,3-butylene glycol 5.0 3. Ethanol 15.0 4. Iron oxide (Bengara) 0.2 5. Zinc oxide 0.5 6. Kaolin 2.0 7. Collagen of Production Example 1 2.0 8. Carrot 50% Propylene Glycol Extract 1.0 9. Placenta Extract 1.0 10. Camphor 0.2 11. Phenol 0.03 12. Preservative (Photosensitizer 201 & 401) Appropriate amount 13. Perfume (Bodhi Tree Water) Appropriate amount 14.Residual water to be 100

(Formulation Example 8) Pack weight% 1. Polyvinyl alcohol 15.0 2. Sodium carboxymethyl cellulose 5.0 3. Propylene glycol 3.0 4. Ethanol 10.0 5. Collagen of Production Example 1 1.0 6. Collagen of Production Example 2 1.0 7. Thyme 50% ethanol extract 1.0 8. Melissa 50% ethanol extract 1.0 9. Preservative (para-hydroxybenzoate) qs 10. flavor (Tokisui) qs 11. Purified water 100 balance

(Formulation Example 9) Peel-off pack (% by weight) 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Pantothenyl ethyl ether 1.0 7. Preparation example 2 Collagen 1.0 8. Safflower 50% ethanol extract 1.0 9. Yuzu 50% ethanol extract 1.0 10. Preservative (paraoxybenzoate) qs 11. Fragrance (cypress water) qs 12. Purified water 100

(Formulation Example 10) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Olive oil 5.0 5. Chamomile oil 5.0 6. Palm oil 5.0 7. Borax 0.5 8. Production example 1 Collagen 1.0 9. Liquid shea fat 1.0 10. Preservative (acrinol) 0.1 11. Perfume (sage water) qs 12. Purified water 100

(Formulation Example 11) Emollient cream% by weight 1. Stearic acid 2.0 2. Stearyl alcohol 7.0 3. Reduced lanolin 2.0 4. Squalane 5.0 5. Octyldodecanol 6.0 6. Polyoxyethylene cetyl ether (25E.O.) 3.0 7. Lipophilic glyceryl monostearate 2.0 8. Propylene glycol 5.0 9. Collagen of Production Example 2 3.0 10. Rosemary 50% 1,3-butylene glycol extract 2.0 11. Preservative (paraben) qs 12. Fragrance ( Cinch water) Appropriate amount 13. Residual water with 100 purified water

(Prescription Example 12) Face Wash Cream% by Weight 1. Myristic acid 25.0 2. Stearic acid 5.0 3. Tallow fatty acid 5.0 4. Propylene glycol 10.0 5. Potassium hydroxide 6.0 6. Coconut oil fatty acid diethanolamide 6.0 7. Production example 1. Collagen 2.0 8. Loofah 45% 1,3-butylene glycol extract 2.0 9. Dipotassium glycyrrhetinate 1.0 10. Preservative (salicylic acid) 0.1 11. Perfume (sage water) qs 12. Purified water 100

(Formulation Example 13) Face wash cream% by weight 1. Stearic acid 10.0 2. Palmitic acid 10.0 3. Myristic acid 12.0 4. Lauric acid 4.0 5. Oleyl alcohol 1.5 6. Lanolin derivative (EO adduct) 1.0 7. Glycerin 18.0 8. Potassium hydroxide 6.0 9. Collagen of Production Example 2 5.0 10. Royal jelly extract 1.0 11. Glycyrrhetinic acid 1.0 12. Preservative (salicylic acid) 0.1 13. Perfume (Tokisui) qs 14. Purified water 100 Residue to do

(Formulation Example 14) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Whey 1.5 5. Collagen of Production Example 1 0.5 6. Amachol 70% 1,3 -Butylene glycol extract 0.5 7. Polyethylene powder 0.5 8. Hydroxypropyl chitosan solution 0.5 9. Amino acids (glycine, glutamine, etc.) 0.5 10. Preservative (undecylenic acid, phenol) qs 11. pH adjuster qs 12. Flavor (lavender) Water) Appropriate amount 13. Residual water with 100 purified water

(Formulation Example 15) Sunscreen cosmetics (O / W type)% by weight 1. Oxybenzone 2.0 2. Octyl paramethoxycinnamate 5.0 3. Squalane 10.0 4. Vaseline 5.0 5. Stearyl alcohol 3.0 6. Stearic acid 3.0 7. Glyceryl monostearate 2.0 8. Polyethyl acrylate 1.0 9.1,3-butylene glycol 6.0 10. Disodium edetate 0.1 11. Triethanolamine 1.0 12. Titanium dioxide 5.0 13. Collagen from Production Example 0.5 14. Honeysuckle 50% dipropylene glycol extract 0.5 15. Saw whisker 50% dipropylene glycol extract 0.5 16. Sicon 50% dipropylene glycol extract 0.5 17. Preservative (paraben) qs 18. Fragrance (apple water) qs 19. purified water 100 and the rest

(Formulation Example 16) Sunscreen cosmetics (oil type) wt% 1. Liquid paraffin 70.0 2. Ethyl paraaminobenzoate 1.0 3. Tetrahydroxybenzophenone 1.0 4. Cetyl octanoate 25.0 5. Collagen 1.0 of Production Example 2 6. Aloe 50% 1,3-butylene glycol extract 0.5 7. Sakinoshita 30% 1,3-butylene glycol extract 0.5 8. Yokuinin 50% 1,3-butylene glycol extract 0.5 9. Bodaiju 45% 1,3 -Butylene glycol extract 0.5 10. Suitable amount of antioxidant (butylhydroxytoluene) 11. Suitable amount of fragrance (Bodaige water)

(Formulation Example 17) Cream foundation (O / W type) weight% 1. stearic acid 4.0 2. glyceryl monostearate 3.0 3. cetanol 1.5 4. isopropyl myristate 7.0 5. liquid paraffin 10.0 6. salami beeswax 3.0 7. Triethanolamine 1.5 8. Glycerin 3.0 9. Bentonite 1.0 10. Titanium oxide 8.0 11.2,4-dihydroxybenzophenone 8.0 12. Kaolin 3.0 13. Talc 3.0 14. Color pigment (iron iron oxide) 1.0 15. Production Example 1 Collagen dry powder 0.2 16. Preservative (paraben) proper amount 17. Fragrance (rose water) proper amount 18. Purified water 100 residue

(Formulation Example 18) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Collagen of Production Example 1 2.0 7. Hydroxypropyl chitosan solution 2.0 8. Mucopolysaccharide solution 1.0 9. Antibacterial and preservative (photosensitive element 101) proper amount 10. pH adjuster proper amount 11. Fragrance (sandalwood water) proper amount 12 .Remaining amount of purified water 100

(Formulation Example 19) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Collagen of Production Example 1.0 6. Nobara 50% 1,2-butylene glycol extract 1.0 7. Futandandelion 50% 1,3-butylene glycol extract 1.0 8. Hydroxyethyl chitosan solution 1.0 9. Trehalose solution 1.0 10. Hydrolyzed silk solution 1.0 11. Antibacterial and preservative (Benzalkonium chloride) Appropriate amount 12.Fragrance (lavender water) Appropriate amount 13.Remaining to be 100 purified water

(Formulation Example 20) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Ethylene glycol 2.0 3. Collagen of Production Example 1 1.0 7. Maronier 50% ethylene glycol extract 1.0 8. Trehalose solution 1.0 9. Hydrolyzed keratin solution 1.0 10. Antibacterial and preservative (benzalkonium chloride) qs 11. perfume (chamomile water) qs 12. purified water 100 and the rest

(Formulation Example 21) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Collagen of Production Example 1 2.0 6. Apricot Fruit or leaf 30% ethanol extract 1.0 7. Melissa hot water extract 1.0 8. Panax ginseng 50% ethanol extract 1.0 9. Carpronium chloride 0.3 10. Hinokitiol 0.5 11. Keratin decomposition solution 1.0 12. Antibacterial and preservative (paraben, Benzalkonium chloride) Appropriate quantity 13. Fragrance (Senkyu water) Appropriate quantity 14. Purified water 100

(Formulation Example 22) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Trimethylene glycol 5.0 6. Production Example 2 Collagen 1.0 7. Parsley 50% 1,3-butylene glycol extract 0.5 8. Loquat leaf 30% ethanol extract 1.0 9. Azulene 1.0 10. Antibacterial and preservative (paraben, benzalkonium chloride) qs 11. Fragrance ( (Touki water) Appropriate amount 12. Residual water with 100 purified water

(Formulation Example 23) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Collagen of Production Example 5.0 5. Peony peel 50% ethanol extraction Product 3.0 6. Yuzu leaf or fruit 50% ethanol extract 3.0 7. Preservative (paraben) suitable amount 8. Purified water 100 residue

(Formulation Example 24) Hair tonic% by weight 1. l-menthol 0.2 2. Ethanol 32.5 3. Polyoxyethylene hydrogenated castor oil 0.5 4. Propylene glycol 5.0 5. Diphenhydramine hydrochloride 0.3 6. Collagen of production example 1.0 7 .50% ethanol extract of Dutch mustard 1.0 8. 50% ethanol extract of pentagon 1.0 9. Carboxymethyl chitin solution 1.0 10. Vitamin E derivative 0.5 11. Antibacterial and preservative (paraben) 0.1 12. Perfume (sage water) 13 .Remaining amount of purified water 100

(Formulation Example 25) Hair restorer / hair restorer weight% 1. Ethanol 60.0 2. Collagen of Production Example 5.0 3. Ginseng 50% propylene glycol extract 2.0 4. Artemisia 50% 1,3-butylene glycol extraction 1.0 5 .50% 1,3-butylene glycol extract of wicker nut 1.0 6. Vitamin E derivative 0.5 7. Pepper tincture 0.5 8. Resorcinol 0.5 9. Dipotassium glycyrrhizinate 0.5 10. Carboxymethyl chitin solution 0.5 11. Beef hematin solution 0.5 12 Mucin solution 0.5 13. Amino acids (glycine, glutamine, etc.) 0.5 14. Antibacterial and preservative (paraben) 0.1 15. Perfume (rosemary water) qs 16. Purified water 100

(Formulation Example 26) Hair restorer / hair restorer weight% 1. Ethanol 60.0 2. Collagen of Production Example 1.0 3. Assembly 60% ethanol extract 1.0 4. Cantharistincini 0.5 5. Pantothenic acid derivative 0.5 6. Glycyrrhizic acid 0.5 7. Resorcinol 0.5 8. Salicylic acid 0.5 9. Hydroxyethyl chitosan solution 0.5 10. Elastin hydrolyzate 0.5 11. Antibacterial and preservative (paraben) 0.1 12. Perfume (lavender water) qs 13. Purified water 100

(Formulation Example 27) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 2.0 4. Dry powder of collagen of Production Example 1 5.0 5. Dry powder of eucalyptus hot water extraction 5.0 6. Suitable amount of pigment (Gunjo green)

(Formulation Example 28) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 4.0 4. Dry powder of collagen of Production Example 2 2.0 5. Drying of collagen of Production Example 1 Powder 1.0 6. Satsuma mandarin 50% ethanol extracted dry powder 1.0 7. Peach leaf 70% ethanol extracted dry powder 1.0 8. Iris 70% ethanol extracted dry powder 1.0 9. Apple juice powder 1.0 10. Pigment (Gunjo, chromium hydroxide) Appropriate amount 11.Fragrance (grape water) Appropriate amount

(Formulation Example 29) Coating protective agent A gauze or liniment cloth is impregnated with a formulation solution prepared by mixing an appropriate amount of collagen, antibiotics, anti-inflammatory and the like of Production Example 2, and attached to the wound. Alternatively, the collagen of Production Example 2 may be directly applied locally and covered with gauze or the like.

[0113]

[Examples] Tests of use of various cosmetic compositions (1) Procedures The emulsions, granule bath preparations, shampoos, rinses, and hair tonics prepared in the examples were used as test items (a), and were tested by male and female panelists (10 persons in total). A use test was performed. As the comparative product (b), a preparation excluding the product of the present invention in the formulation of each external preparation composition was prepared. The test product (a) was used for one month after the start of the test, and then the comparative product (b) was used for the next one month. ) Was used under the following conditions, and a response was requested from a questionnaire. Table 2 shows the results.

(2) Conditions of use Emulsion: applied to face immediately after washing in the morning, immediately after bathing at night. each
3mL, once / day each Granule bath agent: bathing bath, 30g, once / day shampoo: used during hair washing, 10mL, once / day rinse: used immediately after shampoo, 10mL, once / day hair tonic : Use after washing hair, 10mL, once / day

[0115]

[0116]

The cosmetic composition of the present invention has a moisturizing effect,
It has a rough skin improving effect and a hair protecting effect. For the skin, it is used to prevent, reduce or improve dryness, rough skin, cracks, red ants, inflammatory diseases, and the like. Effective for hair, cut hair, gloss imparting, etc.

[Brief description of the drawings]

FIG. 1 is a diagram showing the change over time in the skin electrical conductivity of the product of the present invention.

FIG. 2 is a view showing measurement results of ease of slip and smoothness given to hair of the product of the present invention.

FIG. 3 is a graph showing the results of measuring the permeability of a blank (purified water) into hair.

FIG. 4 is a graph showing the results of measuring the permeability of collagen of Production Example 1 into hair.

FIG. 5 is a graph showing the results of measuring the permeability of collagen of Production Example 2 into hair.

FIG. 6 is a graph showing the results of measuring the permeability of a comparative example (bovine collagen) into hair.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61K 7/06 A61K 7/06 7/075 7/075 7/08 7/08 7/42 7/42 7 / 50 7/50 F term (reference) 4C083 AA082 AA112 AA122 AB032 AB152 AB212 AB232 AB242 AB312 AB352 AB432 AB442 AC022 AC032 AC072 AC102 AC122 AC132 AC182 AC232 AC242 AC332 AC352 AC422 AC432 AC442 AC472 AC482 AC532 AC542 AC552 AC562 AC582 AC642 AC682 AC692 AC902 AD022 AD042 AD092 AD112 AD272 AD312 AD322 AD412 AD422 AD431 AD432 AD442 AD452 AD512 AD532 AD552 AD642 AD662 CC03 CC04 CC05 CC06 CC07 CC19 CC23 CC25 CC32 CC33 CC37 CC38 CC39 DD21 DD22 DD31 DD33 EE06 EE12 EE29

Claims (4)

[Claims] 1. A moisturizer comprising collagen obtained by extracting pigment-free fish skin. 2. A skin roughness improving agent comprising collagen obtained by extracting from pigment-free fish skin. 3. A hair protecting agent comprising collagen obtained by extracting pigment-free fish skin. 4. The humectant according to claim 1, wherein the humectant is a humectant.
A cosmetic composition comprising at least one selected from the group consisting of a skin roughness improving agent according to claim 3 and a hair protecting agent according to claim 3.