JP2002029960A - Agent for reforming dermal collagen fiber bundle and composition containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new dermal collagen fiber bundle-reforming agent for reforming the structure of the dermis collagen fiber bundle. SOLUTION: This dermal collagen fiber bundle-reforming agent, comprising the essence, preferably the polar solvent extract, of a plant belonging to the family Valerianaceae, such as Patrinia villosa (THUNB.) JUSS.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an agent for reconstructing dermal collagen fiber bundles and an external preparation for skin such as cosmetics containing the agent.

[0002]

2. Description of the Related Art Human form changes with age.
Show signs of aging. One such sign on the skin is the formation of wrinkles, and suppression of the formation of such sign is a major theme of cosmetics. Regarding the formation of such wrinkles, the mechanism of occurrence includes 1) loss of elasticity due to rupture or fragmentation of skin elastic fibers such as elastin, and 2) collapse of dermis collagen fiber bundle structure or disorder of its structure. It is said that. Among them, 1) countermeasures such as replenishment of elastin and suppression of disconnection of elastin by soybean protein etc. are mentioned for the loss of elasticity due to rupture and fragmentation of skin elastic fibers such as elastin, 2) With respect to the collapse of the dermis collagen fiber bundle structure or the disorder of the structure, only ursolic acid and its derivatives are known to have the effect of reconstructing the dermis collagen fiber bundle, and the dermal collagen fiber bundle structure is restored. To construct,
Development of a new dermis collagen fiber bundle remodeling agent has been desired.

[0003] On the other hand, Gypsophila is a general term for a group of plants having the same medicinal properties in Chinese medicine in a plurality of families, mainly plants of the scrophulariaceae family, and a representative thereof is Patrinia villosa (THUNB.). JUSS.) And other plants of the Valerianaceae family include, in the essence of the plant, substances having pharmacological effects such as hair growth, hair growth, hair loss prevention, rough skin prevention, anti-inflammatory, analgesic, astringent, hemostatic and anti-allergic effects. It is known to contain, but it was not known to contain any substance that has the effect of reconstructing dermal collagen fiber bundles, and the essence of such plants was used for external application to the skin It has not been known at all that a dermal collagen fiber bundle structure is prepared by incorporating the agent into an agent to improve or prevent wrinkles.

[0004]

SUMMARY OF THE INVENTION The present invention has been made under such circumstances, and it is an object of the present invention to provide a new dermis collagen fiber bundle reconstructing agent for reconstructing a dermal collagen fiber bundle structure. And

[0005]

In view of such a situation, the present inventors have made intensive research efforts in search of a new dermis collagen fiber bundle reconstructing agent for reconstructing the dermis collagen fiber bundle structure. We found such an effect in the essence of Gypsophila and completed the invention. That is, the present invention relates to the following technology. (1) A dermis collagen fiber bundle reconstituting agent comprising the essence of Gypsophila. (2) Valerianacea
e) The dermis collagen fiber bundle remodeling agent according to (1), which is a male pod (Patriniavillosa (THUNB.) JUSS.). (3) The essence of the plant is a whole solvent, or a polar solvent extract or a solvent-removed product thereof consisting of one or more selected from leaves, stems, roots, fruits, seeds, and flowers. The dermal collagen fiber bundle remodeling agent according to (1) or (2), which is characterized in that: (4) A skin external preparation for improving or preventing wrinkles, comprising the dermis collagen fiber bundle remodeling agent according to any one of (1) to (3). (5) The essence of the plant according to (4), wherein the wrinkles are caused by collapse of the dermis collagen fiber bundle or disorder of the structure. (6) An external skin preparation for treating wrinkles caused by collapse of dermal collagen fiber bundles or structural disorder, characterized by containing the essence of Ginseng. (7) Valerianacea (Valerianacea)
e) The external preparation for skin according to (6), which is a man (Patrinia villosa (THUNB.) JUSS.). (8) The essence of the plant is an extract of a polar solvent consisting of one or more kinds selected from leaves, stems, roots, and whole plants or a solvent-removed product thereof,
An external preparation for skin according to (6) or (7). Hereinafter, the present invention will be described in more detail focusing on embodiments.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION (1) Reconstructing Agent for Dermal Collagen Fiber Bundle of the Present Invention The reconstructing agent for dermal collagen fiber bundle of the present invention comprises the essence of Gypsophila. Here, the essence referred to in the present invention is a plant itself, a processed product obtained by drying, shredding, pulverizing, etc. a plant, a plant or a solvent extract obtained by adding a solvent to the processed product, extraction, It is a generic term for the solvent-extracted product obtained by removing the solvent from the product, and the purified product obtained by purifying them by column chromatography or liquid-liquid extraction. As the Chinese ginseng applied to the essence of the present invention, any Chinese ginseng can be used without particular limitation as long as it is classified as Chinese ginseng. For example, Valerianaceae family Patolinia villosa (TH
UNB.) JUSS.), Valerianaceae (Palleniaaceae), Patrinia scabriosaefolia FISCH., Brassicaceae (Cruciferae), Thumbis arvense (Thlaspi arvense)
L.), Compositae Hachijona (Sonchus bra)
chyotus DC.) Asteraceae (Compositae), Taiwan chrysanthemum (Sonchus arvensis L.), Asteraceae (Compositae)
Nogeshi (Sonchus oleraceus L.), Asteraceae (Composita
e) Lxeris chinensis (THUB.)
NAKAI), Compositae Lexeris chinensis Graminifoia (lxeris chinensis NAKAI subs
p. Graminifolia KITAM.), Compositae Lexeris chinensis Versicolor (lxeris chinensis)
NAKAI subsp. Versicolor KITAM.), Asteraceae (Composi)
tae) Lexeris denticul (lxeris denticul)
ata STABB.), and more preferably, Valerianaceae and Patrinia vill.
osa (THUNB.) JUSS.), or Valerianac (Valerianac)
eae) Ominaeshi (Patrinia scabiosaefolia FISCH.)
It is. Although any part of the plant can be used as the part of the plant used for preparing the essence of the present invention, a whole plant with roots is particularly preferred. This is because this site contains many components for reconstructing the dermal collagen fiber bundle. As the essence of the present invention, a polar solvent extract or a solvent-removed product thereof can be particularly preferably exemplified. Examples of the polar solvent include alcohols such as ethanol and methanol, esters such as ethyl acetate and methyl formate, acetone and methyl ethyl ketone. And at least one selected from ketones such as, for example, ethers such as diethyl ether and tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, and water. Of these, particularly preferred are one or more selected from water and alcohol. Such an extract or a solvent-removed product thereof is prepared by adding a 1 to 10-fold amount of a solvent to a plant or a processed product thereof and immersing it for several days at room temperature or several hours at a temperature near the boiling point. I just need. The solvent can be removed by evaporation to dryness, concentration under reduced pressure, or freeze drying. Most preferred are vacuum concentration and lyophilization. In the skin external preparation of the present invention, these dermis collagen fiber bundle remodeling agents may contain only one kind, or may contain two or more kinds, and the preferable content is 0.01 to 10% by weight, more preferably 0.1 to 5% by weight. This is because if the amount is too small, the effect may not be exhibited, and if the amount is too large, the effect may reach a plateau and the flexibility of prescription may be impaired. The dermis collagen fiber bundle remodeling agent of the present invention reconstructs the dermis collagen fiber bundle that has collapsed due to aging, light, or the like, and has an effect of returning to a normal structure and an effect of preventing the dermal collagen fiber bundle structure from being collapsed by light or aging. It has the effect of improving wrinkles and suppressing the formation of wrinkles by such an action. Wrinkles in which the dermal collagen fiber bundle remodeling agent of the present invention exerts its effects are:
It is preferably applied to those caused by collapse of the dermal collagen fiber bundle or structural disorder.

(2) External preparation for skin of the present invention The external preparation for skin of the present invention is characterized by containing the above-mentioned agent for reconstructing dermal collagen fiber bundles. As the external preparation for skin of the present invention, cosmetics, a pharmaceutical composition for external use on skin and the like can be preferably exemplified, and it is particularly preferable to apply to cosmetics.
This is because the action of the dermis collagen fiber bundle remodeling agent of the present invention is moderated, and in addition, the safety is extremely high, so that it is suitable for long-term continuous use. The external preparation for skin of the present invention exerts an excellent effect on improvement and prevention of wrinkles caused by collapse of the dermal collagen fiber bundle or structural disorder due to the effect of the dermis collagen fiber bundle reconstruction agent contained therein.
In addition, even if wrinkles do not occur, it also has the effect of improving or preventing the reduction of texture caused by the same mechanism. In other words, it has the effect of preventing or improving various unfavorable biological events caused by the collapse of the dermal collagen fiber bundle structure or the disorder of the structure, and embodying beautiful skin. Use in such applications is preferred in the skin external preparation of the present invention. What is noteworthy is the effect on wrinkles caused by the collapse of the dermal collagen fiber bundle and the disorder of the structure, and skin external preparations that exhibit such effects are extremely rare. Therefore, the skin external preparation of the present invention,
It is preferable to apply to wrinkles generated by such a mechanism. The external preparation for skin of the present invention may contain, in addition to the agent for reconstructing dermal collagen fiber bundles of the present invention, optional components usually used in external preparations for skin. Such optional components include, for example, hydrocarbons such as squalane, petrolatum, microcrystalline wax, jojoba oil, carnauba wax, esters such as octyldodecyl oleate, triglycerides such as olive oil, tallow, coconut oil, dimethicone, and the like. Silicones such as femethicone and amodimethicone; fatty acids such as stearic acid, oleic acid and ritinoleic acid; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; anionic surface activities such as sulfosuccinates and sodium polyoxyethylene alkyl sulfate. Agents, amphoteric surfactants such as alkyl betaine salts, cationic surfactants such as dialkylammonium salts, sorbitan fatty acid esters, fatty acid monoglycerides, polyoxyethylenes thereof Addendum, polyoxyethylene alkyl ethers, nonionic surfactants such as polyoxyethylene fatty acid esters, polyethylene glycol, glycerine,
Polyhydric alcohols such as 1,3-butanediol, xanthan gum, guar gum, carboxyvinyl polymer, cationized cellulose ether, cationized guar gum, and other thickening / gelling agents, antioxidants, ultraviolet absorbers, fragrances, coloring materials , Preservatives, powders and the like can be preferably exemplified. The external preparation for skin of the present invention can be produced by treating these optional and essential components according to a conventional method. Further, even if the dermis collagen fiber bundle remodeling agent is not contained for the purpose of reconstructing the dermis collagen fiber bundle, if these essences are contained, the action on the wrinkles can be obtained. A topical skin preparation for preventing or improving wrinkles caused by disruption of the dermal collagen fiber bundles or structural disorder contained therein also belongs to the technical scope of the topical skin preparation of the present invention.

[0008]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples, but it goes without saying that the present invention is not limited to only these Examples.

<Example 1> Valerianacea (Valerianacea)
e) 5 l of methanol was added to 500 g of a dried portion of the whole plant with roots of a man-made man (Patrinia villosa (THUNB.) JUSS.), soaked at room temperature for 1 week, filtered and concentrated under reduced pressure. 27g of dermis collagen fiber bundle reconstituting agent 1
Obtained. This was examined in vitro for its dermal collagen fiber bundle remodeling action. That is, a small piece was cut out from the back skin of a hairless mouse (Hr- / Kud, female) that was irradiated with ultraviolet rays at a minimum erythema dose of 3 times / week for 5 consecutive weeks, treated with trypsin, and subjected to skin-derived dermal fibers. The blast cells were collected and used for 5 passages. Culture condition is 5 × 10 4
At a cell concentration of (5 × 10 4) cells / ml, 1.0 mg
/ Ml of acid-soluble collagen was added, and 10-4 (10-4)% of dermis collagen fiber bundle remodeling agent 1 was added to the minimum medium of Eagle supplemented with 10% FBS, followed by culturing for 7 days.
FIG. 1 shows a photograph with 0% added (non-added) (a photograph as a substitute for a drawing), and FIG. 2 shows a photograph with 10-4 (10 −4)% added. Figure 1
In Fig. 2, collagen fibers are distributed randomly, whereas in Fig.
You can see that the structure has begun. This shows that the dermis collagen fiber bundle remodeling agent 1 of the present invention promotes the remodeling of dermal collagen in the dermis.

Example 2 According to the following formulation, a cosmetic (lotion) for preventing or improving wrinkles due to the collapse of the dermal collagen fiber bundle or the disorder of the structure of the present invention was prepared. That is, the prescription component was heated, stirred, solubilized at 70 ° C., and stirred and cooled to obtain a lotion. Dermal collagen fiber bundle reconstituting agent 1 0.1 part by weight Glycerin 5 parts by weight 1,2-pentanediol 4 parts by weight Polyphosphatidyloxyethyl methacrylate 0.1 part by weight Phenoxyethanol 0.1 part by weight Ethanol 5 parts by weight POE (60) Hardened castor oil 0.1 parts by weight Water 85.6 parts by weight

<Example 3> The lotion of Example 2 and the lotion of Comparative Example 1 in which the dermis collagen fiber bundle reconstituting agent 1 of Example 2 was replaced with water were used. Using a panelist who suffers from wrinkles, a long-term continuous test (3 months) was performed, and the improvement of wrinkles was evaluated in three stages of significant, effective, and no effects. A remarkable effect was observed in two subjects, but in Comparative Example 1, only two subjects were effective. From this, it was determined that the skin lotion, which is an external preparation for skin of the present invention, is effective in improving wrinkles. One person who had no effect with the lotion of Example 2 was
It was speculated that the wrinkle formation mechanism does not depend on the collapse of the dermal collagen fiber bundle or the disorder of the structure.

Example 4 An emulsion according to the present invention was prepared according to the following formulation. That is, the components (a), (b) and (c) were each heated to 70 ° C., (b) was neutralized by adding (b) to the mixture, and (c) was gradually added thereto to emulsify. This was stirred and cooled to prepare an emulsion as the external preparation for skin of the present invention. A four-week actual use test was conducted on this panel for 10 panelists.
In 6 persons, a marked improvement in the texture was observed, and in 2 persons, a clear improvement in firmness was observed. There are also two panelists who do not or hardly recognize such an effect, and the mechanism of roughening and reduction of firmness of these panelers is not caused by collapse of the dermal collagen fiber bundle structure or disorder of the structure. Was speculated. A. 1,2-pentanediol 5 parts by weight isoprene glycol 4 parts by weight acrylic acid / alkyl methacrylate polymer 0.6 parts by weight phenoxyethanol 0.1 parts by weight water 30 parts by weight b 10% aqueous solution of potassium hydroxide 3 parts by weight water 41. 3 parts by weight Hassqualane 5 parts by weight Jojoba oil 5 parts by weight Olive oil 5 parts by weight Dermal collagen fiber bundle restructuring agent 1 1 part by weight

Example 5 A pack cosmetic was prepared according to the following formulation. That is, the prescription components were stirred and homogenized to obtain a pack cosmetic. This was effective for improving texture, firmness and wrinkles. Vinyl acetate emulsion 40 parts by weight 1,3-butanediol 5 parts by weight Glycerin 5 parts by weight 1,2-pentanediol 5 parts by weight Dermal collagen fiber bundle remodeling agent 1 5 parts by weight Phenoxyethanol 0.2 parts by weight Ethylparaben 0.1 Parts by weight water 39.7 parts by weight

<Example 6> With respect to other plants of Gypsophila, a dermal collagen fiber bundle remodeling agent of the present invention was prepared in the same manner as in Example 1, and the collagen fiber bundle remodeling action was examined. The collagen fiber bundle remodeling action was as good as collagen fiber bundle remodeling agent 1 as ○, slightly poor as Δ,
When it was not observed (similar to the control), it was evaluated as x. Table 1 shows the results. This shows that the dermis collagen fiber bundle remodeling agent of the present invention has an excellent collagen fiber bundle reconstruction action.

[0015]

[Table 1]

[0016]

According to the present invention, it is possible to provide a new dermis collagen fiber bundle remodeling agent for reconstructing a dermal collagen fiber bundle structure.

[Brief description of the drawings]

FIG. 1 is a view showing test results of an in vitro test of Example 1 without the addition of a dermis collagen fiber bundle remodeling agent.
(Drawing substitute photo)

FIG. 2 is a view showing a test result of an in vitro test of Example 1 in which 10-4% of a dermis collagen fiber bundle remodeling agent was added. (Drawing substitute photo)

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Claims (8)

[Claims] 1. An agent for reconstructing dermal collagen fiber bundles comprising the essence of Gypsophila. 2. The plant of the family Oleoptera (Valeri)
anaceae) Otokoeshi (Patrinia villosa (THUNB.) JUS
S.), wherein the dermal collagen fiber bundle remodeling agent according to claim 1 is used. 3. A polar solvent extract or a solvent-removed product thereof, wherein the essence of the plant body is one or two or more kinds selected from whole plants or leaves, stems, roots, fruits, seeds and flowers. The dermis collagen fiber bundle remodeling agent according to claim 1 or 2, characterized in that: 4. A skin external preparation for improving or preventing wrinkles, comprising the dermis collagen fiber bundle remodeling agent according to any one of claims 1 to 3. 5. The method according to claim 1, wherein the wrinkles are caused by collapse of dermal collagen fiber bundles or structural disorder.
An essence of the plant according to claim 4. 6. A skin external preparation for treating wrinkles caused by collapse of dermal collagen fiber bundles or structural disorder, characterized by containing the essence of Gypsophila. 7. The plant of the present invention is a plant belonging to the family Oleaceae (Vale
rianaceae) Patrinia villosa (THUNB.) J
7. The external preparation for skin according to claim 6, wherein the external preparation is USS. 8. The essence of a plant is an extract of a polar solvent comprising one or more selected from leaves, stems, roots, and whole plants, or a solvent-extracted product thereof. The external preparation for skin according to claim 6 or 7, wherein