JP2002020261A - Agent for restructuring corium collagen fiber bundle and composition containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an agent for restructuring a new corium collagen fiber bundle, capable of restructuring the corium collagen fiber bundle structure for improving or preventing wrinkles. SOLUTION: This corium collagen fiber bundle-restructuring agent consists of an essence, preferably an extract by a polar solvent, of Pulsatilla chinensis (Bunge) regel, etc., belonging to Ranunculaceae.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an agent for reconstructing dermal collagen fiber bundles and an external preparation for skin such as cosmetics containing the agent.

[0002]

2. Description of the Related Art Human form changes with age.
Show signs of aging. One such sign on the skin is the formation of wrinkles, and suppression of the formation of such sign is a major theme of cosmetics. Regarding the formation of such wrinkles, the mechanism of occurrence includes 1) loss of elasticity due to rupture or fragmentation of skin elastic fibers such as elastin, and 2) collapse of dermis collagen fiber bundle structure or disorder of its structure. It is said that. Among them, 1) countermeasures such as replenishment of elastin and suppression of disconnection of elastin by soybean protein etc. are mentioned for the loss of elasticity due to rupture and fragmentation of skin elastic fibers such as elastin, 2) With respect to the collapse of the dermis collagen fiber bundle structure or the disorder of the structure, only ursolic acid and its derivatives are known to have the effect of reconstructing the dermis collagen fiber bundle, and the dermal collagen fiber bundle structure is restored. To construct,
Development of a new dermis collagen fiber bundle remodeling agent has been desired.

[0003] On the other hand, it is already known that Ranunculaceae plants, such as Japanese yellow clover, contain substances having pharmacological actions such as anti-inflammatory, analgesic, astringent, and hemostatic in the plant body. It was not known at all that it contained a substance having an action of reconstructing collagen fiber bundles, and the essence of such a plant was contained in an external preparation for skin to prepare a dermal collagen fiber bundle structure and to improve wrinkles. No improvement or prevention was known at all.

[0004]

SUMMARY OF THE INVENTION The present invention has been made under such circumstances, and it is an object of the present invention to provide a new dermis collagen fiber bundle reconstructing agent for reconstructing a dermal collagen fiber bundle structure. And

[0005]

In view of such a situation, the present inventors have made intensive research efforts in search of a new dermis collagen fiber bundle reconstructing agent for reconstructing the dermis collagen fiber bundle structure. The inventors have found such an effect in the essence of a Ranunculaceae plant and completed the invention. (1) A dermis collagen fiber bundle reconstituting agent comprising the essence of a Ranunculaceae plant. (2) The dermis collagen fiber bundle remodeling agent according to (1), wherein the buttercup plant is Pulsatilla chinensis (Bunge) Regel. (3) The dermis collagen fiber bundle remodeling agent according to (1) or (2), wherein the essence of the plant is a polar solvent extract of the root or a solvent-removed product thereof. (4) An external skin preparation for improving or preventing wrinkles, comprising the dermis collagen fiber bundle remodeling agent according to any one of (1) to (3). (5) The essence of the plant according to (4), wherein the wrinkles are caused by collapse of the dermis collagen fiber bundle or disorder of the structure. (6) A skin external preparation for treating wrinkles caused by collapse of dermal collagen fiber bundles or structural disorder, comprising an essence of a Ranunculaceae plant. (7) The external preparation for skin according to (6), wherein the buttercup family is Pulsatilla chinensis (Bunge) Regel. (8) The essence of a plant is an extract of a polar solvent in a root portion or a solvent-removed product thereof, (6)
Or the external preparation for skin according to (7). Hereinafter, the present invention will be described in more detail focusing on embodiments.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION (1) Dermal Collagen Fiber Bundle Reconstructing Agent of the Present Invention The dermal collagen fiber bundle reconstructing agent of the present invention comprises the essence of a plant of the family Ranunculaceae. Here, the essence referred to in the present invention is a plant itself, a processed product obtained by drying, shredding, pulverizing, etc. a plant, a plant or a solvent extract obtained by adding a solvent to the processed product, extraction, It is a generic term for the solvent-extracted product obtained by removing the solvent from the product, and the purified product obtained by purifying them by column chromatography or liquid-liquid extraction. As the buttercup family plants applied to the essence of the present invention, there is a broad-leafed grass (Pulsatilla chine)
nsis (Bunge) Regel), Anemone tomentosa (Anemon)
e tomentosa (Maxim.) Pei), Anemone SP
ne.sp), Kibunegiku (Anemone japonicia (Thunb) Sieb.e
tZucc) and the like can be preferably exemplified, and among these, Hirosui kinagusa (Pulsatilla chinensis (Bunge) Regel) is particularly preferred. Although any part of the plant can be used as the part of the plant used for adjusting the essence of the present invention, the root is particularly preferable. This is because many components for reconstructing the dermal collagen fiber bundle at this site are contained. As the essence of the present invention, a polar solvent extract or a solvent-removed product thereof can be particularly preferably exemplified. Examples of the polar solvent include alcohols such as ethanol and methanol, esters such as ethyl acetate and methyl formate, acetone and methyl ethyl ketone. And at least one selected from ketones such as, for example, ethers such as diethyl ether and tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, and water. Of these, particularly preferred are one or more selected from water and alcohol. Such an extract or a solvent-removed product thereof is prepared by adding a 1 to 10-fold amount of a solvent to a plant or a processed product thereof and immersing it for several days at room temperature or several hours at a temperature near the boiling point. I just need. The solvent can be removed by evaporation to dryness, concentration under reduced pressure, or freeze drying. Most preferred are vacuum concentration and lyophilization. In the skin external preparation of the present invention,
These dermis collagen fiber bundle reconstituting agents can contain only one kind, or two or more kinds. The preferable content is 0.01 to 10% by weight,
More preferably, it is 0.1 to 5% by weight. This is because if the amount is too small, the effect may not be exhibited, and if the amount is too large, the effect may reach a plateau and the flexibility of prescription may be impaired. The dermis collagen fiber bundle remodeling agent of the present invention reconstructs the dermis collagen fiber bundle that has collapsed due to aging, light, or the like, and has an effect of returning to a normal structure and an effect of preventing the dermal collagen fiber bundle structure from being collapsed by light or aging. It has the effect of improving wrinkles and suppressing the formation of wrinkles by such an action. The wrinkles exerted by the dermis collagen fiber bundle reconstituting agent of the present invention are preferably applied to those caused by collapse of the dermal collagen fiber bundles or structural disorder.

(2) External preparation for skin of the present invention The external preparation for skin of the present invention is characterized by containing the above-mentioned agent for reconstructing dermal collagen fiber bundles. As the external preparation for skin of the present invention, cosmetics, a pharmaceutical composition for external use on skin and the like can be preferably exemplified, and it is particularly preferable to apply to cosmetics.
This is because the action of the dermis collagen fiber bundle remodeling agent of the present invention is moderated, and in addition, the safety is extremely high, so that it is suitable for long-term continuous use. The external preparation for skin of the present invention exerts an excellent effect on improvement and prevention of wrinkles caused by collapse of the dermal collagen fiber bundle or structural disorder due to the effect of the dermis collagen fiber bundle reconstruction agent contained therein.
In addition, even if wrinkles do not occur, it also has the effect of improving or preventing the reduction of texture caused by the same mechanism. In other words, it has the effect of preventing or improving various unfavorable biological events caused by the collapse of the dermal collagen fiber bundle structure or the disorder of the structure, and embodying beautiful skin. Use in such applications is preferred in the skin external preparation of the present invention. What is noteworthy is the effect on wrinkles caused by the collapse of the dermal collagen fiber bundle and the disorder of the structure, and skin external preparations that exhibit such effects are extremely rare. Therefore, the skin external preparation of the present invention,
It is preferable to apply to wrinkles generated by such a mechanism. The external preparation for skin of the present invention may contain, in addition to the agent for reconstructing dermal collagen fiber bundles of the present invention, optional components usually used in external preparations for skin. Such optional components include, for example, hydrocarbons such as squalane, petrolatum, microcrystalline wax, jojoba oil, carnauba wax, esters such as octyldodecyl oleate, triglycerides such as olive oil, tallow, coconut oil, dimethicone, and the like. Silicones such as femethicone and amodimethicone; fatty acids such as stearic acid, oleic acid and ritinoleic acid; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; anionic surface activities such as sulfosuccinates and sodium polyoxyethylene alkyl sulfate. Agents, amphoteric surfactants such as alkyl betaine salts, cationic surfactants such as dialkylammonium salts, sorbitan fatty acid esters, fatty acid monoglycerides, polyoxyethylenes thereof Addendum, polyoxyethylene alkyl ethers, nonionic surfactants such as polyoxyethylene fatty acid esters, polyethylene glycol, glycerine,
Polyhydric alcohols such as 1,3-butanediol, xanthan gum, guar gum, carboxyvinyl polymer, cationized cellulose ether, cationized guar gum, and other thickening / gelling agents, antioxidants, ultraviolet absorbers, fragrances, coloring materials , Preservatives, powders and the like can be preferably exemplified. The external preparation for skin of the present invention can be produced by treating these optional and essential components according to a conventional method. Further, even if the dermis collagen fiber bundle remodeling agent is not contained for the purpose of reconstructing the dermis collagen fiber bundle, if these essences are contained, the action on the wrinkles can be obtained. A topical skin preparation for preventing or improving wrinkles caused by disruption of the dermal collagen fiber bundles or structural disorder contained therein also belongs to the technical scope of the topical skin preparation of the present invention.

[0008]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples, but it goes without saying that the present invention is not limited to only these Examples.

<Example 1> 5 l of methanol was added to 500 g of the root of Ranunculaceae, and the mixture was added at room temperature.
It was immersed for a week, filtered, and concentrated under reduced pressure to obtain 32 g of a dermis collagen fiber bundle remodeling agent 1 of the present invention. This was examined in vitro for its dermal collagen fiber bundle remodeling action. That is, UV irradiation with the minimum erythema dose of 3 times / week
Hairless mice (Hr− / Kud,
A small piece was cut out from the back skin of the (female) and trypsinized to collect dermal fibroblasts derived from the skin and used for 5 passages. The culture conditions were a cell concentration of 5 × 10 4 (5 × 10 4) cells / ml, 1.0 mg / ml of acid-soluble collagen was added, and 0, 10 −4 (10 -4)% of the dermis collagen fiber bundle reconstituting agent was added and cultured for 7 days. FIG. 1 shows a photograph of 0% added (non-added) (substitute drawing) in FIG.
The photograph of addition of 0 to the -4th power) is shown. 1 shows that collagen fibers are randomly distributed, while FIG. 2 shows that a few collagen fibers are gathered together and a structure is being formed. This indicates that the dermis collagen fiber bundle remodeling agent of the present invention promotes the remodeling of dermal collagen in the dermis.

Example 2 According to the following formulation, a cosmetic (lotion) for preventing or improving wrinkles due to the collapse of the dermal collagen fiber bundle or the disorder of the structure of the present invention was prepared. That is, the prescription component was heated, stirred, solubilized at 70 ° C., and stirred and cooled to obtain a lotion. Dermal collagen fiber bundle reconstituting agent 1 0.1 part by weight Glycerin 5 parts by weight 1,2-pentanediol 4 parts by weight Polyphosphatidyloxyethyl methacrylate 0.1 part by weight Phenoxyethanol 0.1 part by weight Ethanol 5 parts by weight POE (60) Hardened castor oil 0.1 parts by weight Water 85.6 parts by weight

<Example 3> The lotion of Example 2 and the lotion of Comparative Example 1 in which the dermis collagen fiber bundle reconstituting agent 1 of Example 2 was replaced with water were used. Using a panelist who suffers from wrinkles, a long-term continuous test (3 months) was performed, and the improvement of wrinkles was evaluated in three stages of significant, effective, and no effects. A remarkable effect was observed in two subjects, but in Comparative Example 1, only two subjects were effective. From this, it was determined that the skin lotion, which is an external preparation for skin of the present invention, is effective in improving wrinkles. One person who had no effect with the lotion of Example 2 was
It was speculated that the wrinkle formation mechanism does not depend on the collapse of the dermal collagen fiber bundle or the disorder of the structure.

Example 4 An emulsion according to the present invention was prepared according to the following formulation. That is, the components (a), (b) and (c) were each heated to 70 ° C., (b) was neutralized by adding (b) to the mixture, and (c) was gradually added thereto to emulsify. This was stirred and cooled to prepare an emulsion as the external preparation for skin of the present invention. A four-week actual use test was conducted on this panel for 10 panelists.
The remarkable improvement of the texture was observed in four persons, and the remarkable improvement of the firmness was recognized in three persons. There are also two panelists who do not or hardly recognize such an effect, and the mechanism of roughening and reduction of firmness of these panelers is not caused by collapse of the dermal collagen fiber bundle structure or disorder of the structure. Was speculated. A. 1,2-pentanediol 5 parts by weight isoprene glycol 4 parts by weight acrylic acid / alkyl methacrylate polymer 0.6 parts by weight phenoxyethanol 0.1 parts by weight water 30 parts by weight b 10% aqueous solution of potassium hydroxide 3 parts by weight water 41. 3 squalane 5 parts by weight Jojoba oil 5 parts by weight Olive oil 5 parts by weight Dermal collagen fiber bundle remodeling agent 1 1 part by weight

Example 5 A pack cosmetic was prepared according to the following formulation. That is, the prescription components were stirred and homogenized to obtain a pack cosmetic. This was effective for improving texture, firmness and wrinkles. Vinyl acetate emulsion 40 parts by weight 1,3-butanediol 5 parts by weight Glycerin 5 parts by weight 1,2-pentanediol 5 parts by weight Dermal collagen fiber bundle remodeling agent 5 parts by weight Phenoxyethanol 0.2 parts by weight Ethyl paraben 0.1 parts by weight Parts water 39.7 parts by weight

[0014]

According to the present invention, it is possible to provide a new dermis collagen fiber bundle remodeling agent for reconstructing a dermal collagen fiber bundle structure.

[Brief description of the drawings]

FIG. 1 is a view showing the test results of the in vitro test of Example 1 without the addition of a dermis collagen fiber bundle remodeling agent.
(Drawing substitute photo)

FIG. 2 is a view showing the test results of the in vitro test of Example 1 in which 10-4% of a dermis collagen fiber bundle remodeling agent was added. (Drawing substitute photo)

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (reference) A61P 17/16 A61P 17/16 43/00 105 43/00 105 F term (reference) 4C083 AA111 AA112 AA122 AB032 AC022 AC102 AC112 AC122 AC172 AC432 AC482 AC892 AD092 CC02 CC04 CC07 EE12 EE13 4C088 AB32 AC11 BA09 BA10 CA05 CA06 CA08 NA14 ZA89 ZC01 ZC41

Claims (8)

[Claims] 1. A dermis collagen fiber bundle reconstituting agent comprising the essence of a Ranunculaceae plant. 2. The plant of the family Ranunculaceae is Pulsatilla chinensis (Bunge) Rege
The dermal collagen fiber bundle remodeling agent according to claim 1, which is l). 3. The method according to claim 3, wherein the essence of the plant is a polar solvent extract of the root or a solvent-removed product thereof.
The dermis collagen fiber bundle remodeling agent according to claim 1 or 2. 4. A skin external preparation for improving or preventing wrinkles, comprising the dermis collagen fiber bundle remodeling agent according to any one of claims 1 to 3. 5. The method according to claim 1, wherein the wrinkles are caused by collapse of dermal collagen fiber bundles or structural disorder.
An essence of the plant according to claim 4. 6. A skin external preparation for treating wrinkles caused by collapse of dermal collagen fiber bundles or structural disorder, comprising an essence of a buttercup plant. 7. The Ranunculaceae plant, wherein the Ranunculaceae plant is Pulsatilla chinensis (Bunge) Rege
The external preparation for skin according to claim 6, which is l). 8. The external preparation for skin according to claim 6, wherein the essence of the plant is an extract of a polar solvent at the root or a solvent-removed product thereof.