JP2002029986A - Corium collagen fiber bundle-restructuring agent and composition containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new corium collagen fiber bundle-restructuring agent adjusting the corium collagen fiber bundle structure for performing the improvement or prevention of wrinkles by incorporating a plant essence in an external agent. SOLUTION: This corium collagen fiber bundle-restructuring agent consists of an essence of a plant of Hypericum erectum THUNB, etc., belonging to Hypericacea, preferably its polar solvent extract.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an agent for reconstructing dermal collagen fiber bundles and an external preparation for skin such as cosmetics containing the agent.

[0002]

2. Description of the Related Art Human form changes with age.
Show signs of aging. One such sign on the skin is the formation of wrinkles, and suppression of the formation of such sign is a major theme of cosmetics. Regarding the formation of such wrinkles, the mechanism of occurrence includes 1) loss of elasticity due to rupture or fragmentation of skin elastic fibers such as elastin, and 2) collapse of dermis collagen fiber bundle structure or disorder of its structure. It is said that. Among them, 1) countermeasures such as replenishment of elastin and suppression of disconnection of elastin by soybean protein etc. are mentioned for the loss of elasticity due to rupture and fragmentation of skin elastic fibers such as elastin, 2) With respect to the collapse of the dermis collagen fiber bundle structure or the disorder of the structure, only ursolic acid and its derivatives are known to have the effect of reconstructing the dermis collagen fiber bundle, and the dermal collagen fiber bundle structure is restored. To construct,
Development of a new dermis collagen fiber bundle remodeling agent has been desired.

On the other hand, Hypericum perforatum (Hyperi)
Hypericaceae (Hyperica) such as cum erectum THUNB.
ceae) plants are based on the essence of the plant, including hair growth, hair growth, hair loss prevention, rough skin prevention, anti-inflammatory, analgesic, astringent, hemostatic,
It is already known that it contains substances having pharmacological actions such as anti-allergic action, but it was not known at all that it contains substances having the action of reconstructing dermal collagen fiber bundles However, it has not been known at all that it is known that the essence of such a plant is contained in an external preparation for skin to prepare a dermal collagen fiber bundle structure and to improve or prevent wrinkles.

[0004]

SUMMARY OF THE INVENTION The present invention has been made under such circumstances, and it is an object of the present invention to provide a new dermis collagen fiber bundle reconstructing agent for reconstructing a dermal collagen fiber bundle structure. And

[0005]

In view of such a situation, the present inventors have made intensive research efforts in search of a new dermis collagen fiber bundle reconstructing agent for reconstructing the dermis collagen fiber bundle structure. The inventors have found such an effect in the essence of the plant of the family Hypericum and completed the invention. That is, the present invention relates to the following technology. (1) A dermis collagen fiber bundle reconstructing agent comprising the essence of a plant of the family Hypericaceae. (2) Plants of the Hypericaceae family (Hypericaceae) include Hypericum erectum THUNB., Hypericaceae (Hypericum perifora)
tum L.), Hypericum perforatum (Hypericum ja
ponicum THUNB.), Hypericum sp. (Hyperi
cum ascyron L.), and the dermis collagen fiber bundle reconstructing agent according to (1), wherein the agent is at least one member selected from the group consisting of: (3) The essence of the plant is a whole solvent, or a polar solvent extract or a solvent-removed product thereof consisting of one or more selected from leaves, stems, roots, fruits, seeds, and flowers. The dermal collagen fiber bundle remodeling agent according to (1) or (2), which is characterized in that: (4) A skin external preparation for improving or preventing wrinkles, comprising the dermis collagen fiber bundle remodeling agent according to any one of (1) to (3). (5) The essence of the plant according to (4), wherein the wrinkles are caused by collapse of the dermis collagen fiber bundle or disorder of the structure. (6) A skin external preparation for treating wrinkles caused by disruption of dermal collagen fiber bundles or structural disorder, comprising an essence of a plant belonging to the family Hypericaceae. (7) The plant of the Hypericaceae family is Hypericaceae (Hypericum erectum THUN)
B.), Hypericum perforatum (Hypericum)
periforatum L.), Hypericum perforatum (Hyp)
ericum japonicum THUNB.) and one or two selected from Hypericum ascyron L.
The external preparation for skin according to (6), which is at least one kind. (8) The essence of the plant is an extract of a polar solvent consisting of one or more selected from leaves, stems, roots, and whole plants or a solvent-removed product thereof.
An external preparation for skin according to (6) or (7). Hereinafter, the present invention will be described in more detail focusing on embodiments.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION (1) Dermal Collagen Fiber Bundle Reconstructing Agent of the Present Invention The dermal collagen fiber bundle reconstructing agent of the present invention comprises the essence of a plant of the family Hypericum. Here, the essence referred to in the present invention is a plant itself, a processed product obtained by drying, shredding, pulverizing, etc. a plant, a plant or a solvent extract obtained by adding a solvent to the processed product, extraction, It is a generic term for the solvent-extracted product obtained by removing the solvent from the product, and the purified product obtained by purifying them by column chromatography or liquid-liquid extraction. The plants of the family Hypericaceae applied to the essence of the present invention include Hypericaceae (Hypericum erectum THUNB.) And Hypericaceae (Hypericum periforatum).
L.), Hypericum (Hypericum japoni)
cum THUNB.), Hypericum sp. (Hypericum)
ascyron L.) and the like, and among them, Hypericum (Hypericum erectum THUNB.) is the most preferred. Any part of the plant can be used as the part of the plant used for preparing the essence of the present invention, but a particularly preferable one is a dried whole plant. This is because this site contains many components for reconstructing the dermal collagen fiber bundle. As the essence of the present invention, a polar solvent extract or a solvent-removed product thereof can be particularly preferably exemplified. Examples of the polar solvent include alcohols such as ethanol and methanol, esters such as ethyl acetate and methyl formate, acetone and methyl ethyl ketone. And at least one selected from ketones such as, for example, ethers such as diethyl ether and tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, and water. Of these, particularly preferred are one or more selected from water and alcohol. Such an extract or a solvent-removed product thereof is prepared by adding a 1 to 10-fold amount of a solvent to a plant or a processed product thereof and immersing it for several days at room temperature or several hours at a temperature near the boiling point. I just need. The solvent can be removed by evaporation to dryness, concentration under reduced pressure, or freeze drying. Most preferred are vacuum concentration and lyophilization. In the skin external preparation of the present invention, these dermis collagen fiber bundle remodeling agents may contain only one kind, or may contain two or more kinds, and the preferable content is 0.01 to 10
% By weight, more preferably 0.1 to 5% by weight. This is because if the amount is too small, the effect may not be exhibited, and if the amount is too large, the effect may reach a plateau and the flexibility of prescription may be impaired. The dermis collagen fiber bundle remodeling agent of the present invention reconstructs the dermis collagen fiber bundle that has collapsed due to aging, light, or the like, and has an effect of returning to a normal structure and an effect of preventing the dermal collagen fiber bundle structure from being collapsed by light or aging. It has the effect of improving wrinkles and suppressing the formation of wrinkles by such an action.
The wrinkles exerted by the dermis collagen fiber bundle reconstituting agent of the present invention are preferably applied to those caused by collapse of the dermal collagen fiber bundles or structural disorder.

(2) External preparation for skin of the present invention The external preparation for skin of the present invention is characterized by containing the above-mentioned agent for reconstructing dermal collagen fiber bundles. As the external preparation for skin of the present invention, cosmetics, a pharmaceutical composition for external use on skin and the like can be preferably exemplified, and it is particularly preferable to apply to cosmetics.
This is because the action of the dermis collagen fiber bundle remodeling agent of the present invention is moderated, and in addition, the safety is extremely high, so that it is suitable for long-term continuous use. The external preparation for skin of the present invention exerts an excellent effect on improvement and prevention of wrinkles caused by collapse of the dermal collagen fiber bundle or structural disorder due to the effect of the dermis collagen fiber bundle reconstruction agent contained therein.
In addition, even if wrinkles do not occur, it also has the effect of improving or preventing the reduction of texture caused by the same mechanism. In other words, it has the effect of preventing or improving various unfavorable biological events caused by the collapse of the dermal collagen fiber bundle structure or the disorder of the structure, and embodying beautiful skin. Use in such applications is preferred in the skin external preparation of the present invention. What is noteworthy is the effect on wrinkles caused by the collapse of the dermal collagen fiber bundle and the disorder of the structure, and skin external preparations that exhibit such effects are extremely rare. Therefore, the skin external preparation of the present invention,
It is preferable to apply to wrinkles generated by such a mechanism. The external preparation for skin of the present invention may contain, in addition to the agent for reconstructing dermal collagen fiber bundles of the present invention, optional components usually used in external preparations for skin. Such optional components include, for example, hydrocarbons such as squalane, petrolatum, microcrystalline wax, jojoba oil, carnauba wax, esters such as octyldodecyl oleate, triglycerides such as olive oil, tallow, coconut oil, dimethicone, and the like. Silicones such as femethicone and amodimethicone; fatty acids such as stearic acid, oleic acid and ritinoleic acid; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; anionic surface activities such as sulfosuccinates and sodium polyoxyethylene alkyl sulfate. Agents, amphoteric surfactants such as alkyl betaine salts, cationic surfactants such as dialkylammonium salts, sorbitan fatty acid esters, fatty acid monoglycerides, polyoxyethylenes thereof Addendum, polyoxyethylene alkyl ethers, nonionic surfactants such as polyoxyethylene fatty acid esters, polyethylene glycol, glycerine,
Polyhydric alcohols such as 1,3-butanediol, xanthan gum, guar gum, carboxyvinyl polymer, cationized cellulose ether, cationized guar gum, and other thickening / gelling agents, antioxidants, ultraviolet absorbers, fragrances, coloring materials , Preservatives, powders and the like can be preferably exemplified. The external preparation for skin of the present invention can be produced by treating these optional and essential components according to a conventional method. Further, even if the dermis collagen fiber bundle remodeling agent is not contained for the purpose of reconstructing the dermis collagen fiber bundle, if these essences are contained, the action on the wrinkles can be obtained. A topical skin preparation for preventing or improving wrinkles caused by disruption of the dermal collagen fiber bundles or structural disorder contained therein also belongs to the technical scope of the topical skin preparation of the present invention.

[0008]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples, but it goes without saying that the present invention is not limited to only these Examples.

<Example 1> Hypericaceae (Hypericaceae)
ae) 5 g of methanol was added to 500 g of dried whole plant of Hypericum erectum THUNB., soaked at room temperature for 1 week, filtered, concentrated under reduced pressure, and the dermal collagen fiber bundle reconstituting agent 1 of the present invention was added. Was obtained. This was examined in vitro for its dermal collagen fiber bundle remodeling action. That is, a hairless mouse (Hr− / Ku) that was irradiated with ultraviolet light at a minimum erythema dose of 3 times / week for 5 consecutive weeks.
(d, female), a small piece was cut out from the back skin, treated with trypsin to collect dermal fibroblasts derived from the skin, and used for 5 passages. Culture conditions were 5 × 10 4 (5 × 10 4
A cell concentration of 10 / ml, 1.0 mg / ml of acid-soluble collagen was added, and a 10-4 (10-4)% dermis collagen fiber bundle remodeling agent was added in a minimum medium of 10% FBS-added Eagle. 1 was added and cultured for 7 days. FIG. 1 shows a photograph of 0% added (non-added) (substitute drawing) in FIG.
The photograph of addition of 0 to the -4th power) is shown. 1 shows that collagen fibers are randomly distributed, while FIG. 2 shows that a few collagen fibers are gathered together and a structure is being formed. This shows that the dermis collagen fiber bundle remodeling agent 1 of the present invention promotes the remodeling of dermal collagen in the dermis.

Example 2 According to the following formulation, a cosmetic (lotion) for preventing or improving wrinkles due to the collapse of the dermal collagen fiber bundle or the disorder of the structure of the present invention was prepared. That is, the prescription component was heated, stirred, solubilized at 70 ° C., and stirred and cooled to obtain a lotion. Dermal collagen fiber bundle reconstituting agent 1 0.1 part by weight Glycerin 5 parts by weight 1,2-pentanediol 4 parts by weight Polyphosphatidyloxyethyl methacrylate 0.1 part by weight Phenoxyethanol 0.1 part by weight Ethanol 5 parts by weight POE (60) Hardened castor oil 0.1 parts by weight Water 85.6 parts by weight

<Example 3> The lotion of Example 2 and the lotion of Comparative Example 1 in which the dermis collagen fiber bundle reconstituting agent 1 of Example 2 was replaced with water were used. Using a panelist suffering from wrinkles, a long-term continuous test (3 months) was performed, and the improvement of wrinkles was evaluated in three stages of significant, effective, and no effects. A remarkable effect was observed in one person, and an effect was observed in one person. However, in Comparative Example 1, only two persons were effective. From this, it was determined that the skin lotion, which is an external preparation for skin of the present invention, is effective in improving wrinkles. One person who had no effect with the lotion of Example 2 was
It was speculated that the wrinkle formation mechanism does not depend on the collapse of the dermal collagen fiber bundle or the disorder of the structure.

Example 4 An emulsion according to the present invention was prepared according to the following formulation. That is, the components (a), (b) and (c) were each heated to 70 ° C., (b) was neutralized by adding (b) to the mixture, and (c) was gradually added thereto to emulsify. This was stirred and cooled to prepare an emulsion as the external preparation for skin of the present invention. A four-week actual use test was conducted on this panel for 10 panelists.
In 5 persons, a marked improvement in the texture was observed, and in 3 persons, a clear improvement in firmness was recognized. There are also two panelists who do not or hardly recognize such an effect, and the mechanism of roughening and reduction of firmness of these panelers is not caused by collapse of the dermal collagen fiber bundle structure or disorder of the structure. Was speculated. A. 1,2-pentanediol 5 parts by weight isoprene glycol 4 parts by weight acrylic acid / alkyl methacrylate polymer 0.6 parts by weight phenoxyethanol 0.1 parts by weight water 30 parts by weight b 10% aqueous solution of potassium hydroxide 3 parts by weight water 41. 3 parts by weight Hassqualane 5 parts by weight Jojoba oil 5 parts by weight Olive oil 5 parts by weight Dermal collagen fiber bundle restructuring agent 1 1 part by weight

Example 5 A pack cosmetic was prepared according to the following formulation. That is, the prescription components were stirred and homogenized to obtain a pack cosmetic. This was effective for improving texture, firmness and wrinkles. Vinyl acetate emulsion 40 parts by weight 1,3-butanediol 5 parts by weight Glycerin 5 parts by weight 1,2-pentanediol 5 parts by weight Dermal collagen fiber bundle remodeling agent 1 5 parts by weight Phenoxyethanol 0.2 parts by weight Ethylparaben 0.1 Parts by weight water 39.7 parts by weight

Example 6 A dermal collagen fiber bundle remodeling agent of the present invention was prepared for other plants of the family Hypericum in the same manner as in Example 1, and the collagen fiber bundle remodeling action was examined. The collagen fiber bundle remodeling action was as good as collagen fiber bundle remodeling agent 1 as ○, slightly poor as Δ,
When it was not observed (similar to the control), it was evaluated as x. Table 1 shows the results. This shows that the dermis collagen fiber bundle remodeling agent of the present invention has an excellent collagen fiber bundle reconstruction action.

[0015]

[Table 1]

[0016]

According to the present invention, it is possible to provide a new dermis collagen fiber bundle remodeling agent for reconstructing a dermal collagen fiber bundle structure.

[Brief description of the drawings]

FIG. 1 is a view showing test results of an in vitro test of Example 1 without the addition of a dermis collagen fiber bundle remodeling agent.
(Drawing substitute photo)

FIG. 2 is a view showing a test result of an in vitro test of Example 1 in which 10-4% of a dermis collagen fiber bundle remodeling agent was added. (Drawing substitute photo)

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Claims (8)

[Claims] 1. A dermis collagen fiber bundle reconstructing agent comprising the essence of a plant belonging to the family Hypericaceae. 2. The plant of the Hypericaceae family (Hypericaceae) includes Hypericaceae (Hypericum er).
ectum THUNB.), Hypericum perforatum L., Hypericum periforatum L., Hypericum japonicum THUNB., Hypericum ascyron L.
The dermis collagen fiber bundle remodeling agent according to claim 1, wherein the agent is a kind or two or more kinds. 3. A polar solvent extract or a solvent-removed product thereof, wherein the essence of the plant body is one or two or more kinds selected from whole plants or leaves, stems, roots, fruits, seeds and flowers. The dermis collagen fiber bundle remodeling agent according to claim 1 or 2, characterized in that: 4. A skin external preparation for improving or preventing wrinkles, comprising the dermis collagen fiber bundle remodeling agent according to any one of claims 1 to 3. 5. The method according to claim 1, wherein the wrinkles are caused by collapse of dermal collagen fiber bundles or structural disorder.
An essence of the plant according to claim 4. 6. A skin external preparation for treating wrinkles caused by disruption of dermal collagen fiber bundles or structural disorder, comprising an essence of a plant of the family Hypericaceae. 7. The plant of the Hypericaceae family (Hypericaceae) is Hypericum erectum THUNB., Hypericaceae (Hypericumperiforat).
um L.), Hypericum (Hypericum jap)
onicum THUNB.), Hypericum
um ascyron L.), the external preparation for skin according to claim 6, wherein the external preparation is one or two or more selected from the group consisting of: 8. The essence of a plant is an extract of a polar solvent comprising one or more selected from leaves, stems, roots, and whole plants, or a solvent-extracted product thereof. The external preparation for skin according to claim 6 or 7, wherein