JP4355091B2 - Dermal collagen fiber bundle reconstituting agent and composition containing the same - Google Patents

Description

【００１６】
【発明の効果】
本発明によれば、真皮コラーゲン線維束構造を再構築する、新たな真皮コラーゲン線維束再構築剤を提供することができる。
【図面の簡単な説明】
【図１】 実施例１のイン・ビトロ試験の真皮コラーゲン線維束再構築剤無添加の試験結果を示す図である。（図面代用写真）
【図２】 実施例１のイン・ビトロ試験の真皮コラーゲン線維束再構築剤１０-4％添加の試験結果を示す図である。（図面代用写真）[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a dermis collagen fiber bundle reconstituting agent and an external preparation for skin such as cosmetics containing the same.
[0002]
[Prior art]
Humans change shape with age and show signs of aging. One of such signs in the skin is the formation of wrinkles, and the suppression of the formation of such signs is a major theme of cosmetics. The formation mechanism of such wrinkles includes 1) the loss of elasticity due to rupture and fragmentation of skin elastic fibers such as elastin, and 2) the collapse of the dermal collagen fiber bundle structure or the disorder of the structure. It is said that. Among them, 1) Regarding the loss of elasticity due to rupture and fragmentation of skin elastic fibers such as elastin, countermeasures such as replenishment of elastin and suppression of rupture of elastin by soy protein are mentioned. For the disruption or disruption of the structure of the dermal collagen fiber bundle, only ursolic acid and its derivatives are known to have a remodeling effect on the dermal collagen fiber bundle. Development of a new dermal collagen fiber bundle restructuring agent to be constructed has been desired.
[0003]
On the other hand, Hishosou is a general term for a group of plants having the same medicinal effect in Chinese medicines across multiple families centering on the Ominae family, and these representative Otoshie (Patrinia villosa (THUNB.) JUSS.) Plants of the Valerianaceae family include substances with pharmacological effects such as hair growth, hair restoration, hair loss prevention, rough skin prevention, anti-inflammatory, analgesic, astringent, hemostatic, antiallergic effects, etc. in the plant essence Although it is already known, it was not known at all that it contains a substance having an action of reconstructing the dermal collagen fiber bundle, and such a plant essence is contained in an external preparation for skin. In addition, it has not been known at all that the dermal collagen fiber bundle structure is adjusted to improve or prevent wrinkles.
[0004]
[Problems to be solved by the invention]
The present invention has been made under such circumstances, and an object of the present invention is to provide a new dermal collagen fiber bundle restructuring agent that reconstructs the dermal collagen fiber bundle structure.
[0005]
[Means for solving problems]
In view of such a situation, the present inventors have made intensive research efforts for a new dermal collagen fiber bundle restructuring agent that reconstructs the dermal collagen fiber bundle structure. We found such an action and completed the invention. That is, the present invention relates to the following technique.
(1) Oh Minaeshi family (Valerianaceae) Otokoeshi (Patrinia villosa (THUNB.) JUSS .) Containing the true skin collagen fiber bundles rebuild agent made from essence of, wrinkle improvement or skin external agent for prevention.
(2) The essence of Otokoeshi is a polar solvent extract or a solvent-removed product thereof composed of one or more selected from whole plants or leaves, stems, roots, fruits, seeds and flowers. The skin external preparation as described in ( 1) characterized by the above-mentioned.
[0006]
DETAILED DESCRIPTION OF THE INVENTION
(1) Dermal Collagen Fiber Bundle Reconstructing Agent of the Present Invention The dermal collagen fiber bundle reconstituting agent of the present invention comprises the essence of Hisho-so. Here, the essence referred to in the present invention is the plant itself, a processed product obtained by drying, chopping, and pulverizing the plant, a solvent extract obtained by adding a solvent to the plant or the processed product, extraction It is a generic term for a solvent-removed product of an extract obtained by removing a solvent from a product, a purified product obtained by purifying the product by column chromatography, liquid-liquid extraction, or the like. As the high sage that is applied to the essence of the present invention, it can be used without particular limitation as long as it is classified into high sage in the Kampo classification, for example, Valerianaceae, Patrinia villosa (THUNB.) JUSS .) 、 Valerianaceae 、 Parinia scabriosaefolia FISCH. 、 Cruciferae 、 Thlaspi arvense L. 、 Compositae 、 Sonchus brachyotus DC. 、 Compositae (Sonchus arvensis L.), Asteraceae (Compositae) Nogeshi (Sonchus oleraceus L.), Asteraceae (Compositae) Lexeris chinensis (lxeris chinensis (THUB.) NAKAI), Asteraceae (Compositae) Lexeris chinensis sp. Graminifolia KITAM.), Compositae Lexeris chinensis versicolor (lxeris chinensis N AKAI subsp. Versicolor KITAM.), Compositae Lexeris denticulata (lxeris denticulata STABB.) Can be preferably exemplified, and more preferably, Valerianaceae patrinia villosa (THUNB.) JUSS. It is a family (Valerianaceae) Ominaeshi (Patrinia scabiosaefolia FISCH.). As the plant part used for the preparation of the essence of the present invention, any part of the plant body can be used, but particularly preferred is the whole plant with roots. This is because the site contains many components for reconstructing the dermal collagen fiber bundle. Further, as the essence of the present invention, a polar solvent extract or a solvent-removed product thereof can be particularly preferably exemplified. Examples of the polar solvent include alcohols such as ethanol and methanol, esters such as ethyl acetate and methyl formate, acetone and methyl ethyl ketone. Preferred examples include one or more selected from ketones such as diethyl ether and ethers such as tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, and water. Of these, particularly preferred are one or more selected from water and alcohol. Such an extract or its solvent-removed product is soaked in a plant or processed product by 1 to 10 times the amount of solvent and immersed for several days at room temperature or for several hours at a temperature near the boiling point. That's fine. The solvent can be removed by evaporation to dryness, concentration under reduced pressure, or lyophilization. Most preferred are vacuum concentration and lyophilization. In the external preparation for skin of the present invention, these dermis collagen fiber bundle reconstructing agents can contain only one species or two or more species, and the preferred content is 0.01 to An example is 10% by weight, more preferably 0.1 to 5% by weight. This is because if the amount is too small, the effect may not be exhibited, and if the amount is too large, the effect reaches a peak and the degree of freedom of prescription may be impaired. The dermis collagen fiber bundle restructuring agent of the present invention reconstructs the dermis collagen fiber bundle that has been destroyed by aging, light, etc., and returns it to a normal structure, and prevents the dermis collagen fiber bundle structure from being destroyed by light or aging. And has the effect of improving wrinkles and suppressing the formation of wrinkles. The wrinkles exerted by the dermis collagen fiber bundle restructuring agent of the present invention are preferably applied to those caused by disruption of the dermis collagen fiber bundle or disorder of the structure.
[0007]
(2) External preparation for skin of the present invention The external preparation for skin of the present invention is characterized by containing the dermis collagen fiber bundle reconstructing agent. As the skin external preparation of the present invention, cosmetics, skin external pharmaceutical compositions and the like can be preferably exemplified, and among them, application to cosmetics is particularly preferable. This is because the action of the dermis collagen fiber bundle restructuring agent of the present invention is moderate and, in addition, the safety of this product is extremely high, so that it is suitable for long-term continuous use. The external preparation for skin of the present invention exhibits an excellent action for improving and preventing wrinkles caused by collapse of the dermal collagen fiber bundle or disorder of the structure due to the effect of the dermis collagen fiber bundle restructuring agent contained therein. In addition, even if it does not lead to wrinkles, it shows an action to improve or prevent any decrease in texture caused by the same mechanism. In other words, the present invention has an effect of embodying beautiful skin by preventing or improving various unfavorable events caused by the collapse of the dermal collagen fiber bundle structure and the disorder of the structure. It is preferable to use for such applications in the external preparation for skin of the present invention. Of particular note is the effect on wrinkles caused by the disruption of the dermal collagen fiber bundle and the disruption of the structure, and skin external preparations that exhibit such an effect are extremely rare. Therefore, the external preparation for skin of the present invention is preferably applied to wrinkles that occur by such a mechanism. In the external preparation for skin of the present invention, in addition to the dermis collagen fiber bundle reconstructing agent of the present invention, it can contain optional components usually used in external preparations for skin. Such optional components include, for example, hydrocarbons such as squalane, petrolatum, microcrystalline wax, esters such as jojoba oil, carnauba wax, octyldodecyl oleate, triglycerides such as olive oil, beef tallow, coconut oil, dimethicone, Silicones such as femethicone and amodimethicone, fatty acids such as stearic acid, oleic acid, and lithinoleic acid, higher alcohols such as oleyl alcohol, stearyl alcohol, octyldodecanol, anionic surface activity such as sulfosuccinic acid ester and sodium polyoxyethylene alkylsulfate Agents, amphoteric surfactants such as alkylbetaine salts, cationic surfactants such as dialkylammonium salts, sorbitan fatty acid esters, fatty acid monoglycerides, polyoxyethylenes thereof Additives, nonionic surfactants such as polyoxyethylene alkyl ethers and polyoxyethylene fatty acid esters, polyhydric alcohols such as polyethylene glycol, glycerin and 1,3-butanediol, xanthan gum, guar gum, carboxyvinyl polymer, cation Preferred examples include thickening / gelling agents such as sulfonated cellulose ether and cationized guar gum, antioxidants, ultraviolet absorbers, fragrances, coloring materials, preservatives, and powders. The skin external preparation of this invention can manufacture the skin external preparation of this invention by processing these arbitrary components and an essential component in accordance with a conventional method. Even if the dermis collagen fiber bundle restructuring agent is not contained for the purpose of reconstructing the dermis collagen fiber bundle, if these essences are contained, the effect on the wrinkles can be obtained. The skin external preparation for preventing or improving wrinkles caused by the collapse of the dermal collagen fiber bundle or the disorder of the structure is also within the technical scope of the skin external preparation of the present invention.
[0008]
【Example】
Hereinafter, the present invention will be described in more detail with reference to examples, but it goes without saying that the present invention is not limited only to these examples.
[0009]
<Example 1>
Add 5 liters of methanol to 500 g of dry dried whole grass rooted in Valerianaceae (Patrinia villosa (THUNB.) JUSS.), Soak for 1 week at room temperature, filter, and concentrate under reduced pressure. 27 g of dermal collagen fiber bundle reconstituting agent 1 of the present invention was obtained. About this thing, the dermis collagen fiber bundle reconstruction effect | action was investigated in vitro. That is, a small piece was cut out from the dorsal skin of a hairless mouse (Hr- / Kud, female) that had been subjected to UV irradiation with a minimum amount of erythema 3 times / week for 5 consecutive weeks, and dermal fibers derived from the skin by trypsinization. Blast cells were collected and used after 5 passages. The culture conditions were 5 × 10 4 (5 × 10 4) cells / ml, 1.0 mg / ml acid-soluble collagen was added, 10 −4 (10 − 4th power)% dermal collagen fiber bundle reconstituting agent 1 was added and cultured for 7 days. FIG. 1 shows a photograph of 0% added (non-added) (drawing substitute photograph), and FIG. 2 shows a photograph of 10-4 (10 to the fourth power) added. In FIG. 1, collagen fibers are randomly distributed, whereas in FIG. 2, two or three collagen fibers are gathered to start making a structure. Thereby, it turns out that the dermis collagen fiber bundle restructuring agent 1 of the present invention promotes the dermis collagen to rebuild in the dermis.
[0010]
<Example 2>
According to the formulation shown below, a cosmetic (skin lotion) for preventing or improving wrinkles caused by the collapse of the dermal collagen fiber bundle or the disorder of the structure of the present invention was prepared. That is, the prescription ingredients were heated, stirred and solubilized at 70 ° C., stirred and cooled to obtain a lotion.
Dermal collagen fiber bundle restructuring agent 1 0.1 parts by weight glycerin 5 parts by weight 1,2-pentanediol 4 parts by weight polyphosphatidyloxyethyl methacrylate 0.1 parts by weight phenoxyethanol 0.1 parts by weight ethanol 5 parts by weight POE (60) Hardened castor oil 0.1 parts by weight Water 85.6 parts by weight
<Example 3>
With respect to the lotion of Example 2 and the lotion of Comparative Example 1 in which the dermis collagen fiber bundle reconstituting agent 1 of the lotion of Example 2 is replaced with water, using a panelist suffering from wrinkles of 5 people per group, A long-term continuous test (3 months) was conducted, and the improvement of wrinkles was evaluated in three stages: effective, effective, and ineffective. In the use test group of Example 2, 2 patients were effective, and 2 were effective. However, in Comparative Example 1, only two people were effective. From this, it was judged that the skin lotion which is an external preparation for skin of the present invention is effective in improving wrinkles. In addition, it was speculated that one person who had no effect with the lotion of Example 2 did not have a wrinkle formation mechanism due to the collapse of the dermal collagen fiber bundle or the disorder of the structure.
[0012]
<Example 4>
The emulsion of the present invention was prepared according to the formulation shown below. That is, the components of A, B and C were heated to 70 ° C., respectively, and B was added to neutralize, and C was gradually added to emulsify. This was stirred and cooled to prepare an emulsion which is an external preparation for skin of the present invention. When this was subjected to a 10-week actual use test for 10 panelists, 6 persons were observed to have improved skin texture, and 2 persons were able to clearly improve the elasticity. In addition, there are two panelists who have no or almost no such effect, and that the texture of these panelers and the mechanism of lowering the tension are not caused by the collapse of the dermal collagen fiber bundle structure or the disorder of the structure. Was guessed.
A) 1,2-pentanediol 5 parts by weight isoprene glycol 4 parts by weight acrylic acid / alkyl methacrylate polymer 0.6 part by weight phenoxyethanol 0.1 part by weight water 30 parts by weight 10% aqueous potassium hydroxide solution 3 parts by weight water 41. 3 parts by weight Husqualane 5 parts by weight jojoba oil 5 parts by weight olive oil 5 parts by weight Dermal collagen fiber bundle reconstituting agent 1 1 part by weight
<Example 5>
A pack cosmetic was prepared according to the prescription shown below. That is, the prescription ingredients were stirred and homogenized to obtain a pack cosmetic. This was effective in improving texture, firmness and wrinkles.
Vinyl acetate emulsion 40 parts by weight 1,3-butanediol 5 parts by weight glycerin 5 parts by weight 1,2-pentanediol 5 parts by weight Dermal collagen fiber bundle reconstituting agent 1 5 parts by weight Phenoxyethanol 0.2 parts by weight Ethylparaben 0.1 Parts by weight water 39.7 parts by weight
<Example 6>
With respect to other plants of Hisho-so, the dermis collagen fiber bundle restructuring agent of the present invention was prepared in the same manner as in Example 1, and the collagen fiber bundle rebuilding action was examined. Collagen fiber bundle remodeling action was evaluated as ○ for the same degree as collagen fiber bundle restructuring agent 1, Δ for slightly inferior, and x for not recognized (same as control). The results are shown in Table 1. From this, it can be seen that the dermal collagen fiber bundle restructuring agent of the present invention has an excellent collagen fiber bundle rebuilding action.
[0015]
[Table 1]

[0016]
【The invention's effect】
ADVANTAGE OF THE INVENTION According to this invention, the new dermal collagen fiber bundle reconstruction agent which reconstructs a dermal collagen fiber bundle structure can be provided.
[Brief description of the drawings]
FIG. 1 is a view showing the test results of the in vitro test of Example 1 without addition of a dermal collagen fiber bundle reconstituting agent. (Drawing substitute photo)
FIG. 2 is a view showing the test results of addition of 10-4% of a dermal collagen fiber bundle reconstructing agent in the in vitro test of Example 1. (Drawing substitute photo)

Claims (2)

Oh Minaeshi Department (Valerianaceae) Otokoeshi (Patrinia villosa (THUNB.) JUSS .) Containing the true skin collagen fiber bundles rebuild agent made from essence of, wrinkle improvement or skin external agent for prevention. The essence of Otokoeshi is a polar solvent extract comprising one or more selected from whole plants or leaves, stems, roots, fruits, seeds and flowers, or a solvent removal product thereof. The external preparation for skin according to claim 1 .

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