JP2002027972A - Method for cultivating sterile ulva pertusa - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for cultivating sterile Ulva pertusa, capable of effectively cultivating the Ulva pertusa in a cultivating tank for a short time. SOLUTION: This method for cultivating the sterile Ulva pertusa comprises floating to cultivate the Ulva pertusa with water current rotating in the cultivating tank, by using the cultivating tank which is equipped with a cylindrical inner sleeve 3 positioned in the central area of a cylindrical tank and having openings 4 on its circumferential surface, an overflow-type drain 5 positioned in the cylindrical inner sleeve 3, and a water-filler 7 for generating the rotating water current along the circumferential inner surface of the cylindrical tank 1.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating seaweed, in particular, a method for efficiently cultivating a large amount of sterile anaosa, which is a kind of highly proliferating seaweed.

[0002]

2. Description of the Related Art Unlike a large seaweed, a sterile Anaaosa, which is a mutant of Anaaosa, does not change generation from sporophyte to gametophyte. Due to its high speed, it has been widely used as a fish culture feed or food additive.

[0003] Sterile Anaaosa grows well in the high water temperature period from summer to autumn when the water temperature is 20 to 30 ° C. Therefore, it is possible to cultivate a large amount under such temperature conditions, but it is still a year. There is no established culture method that enables efficient mass cultivation through cultivation.

[0004] For example, a method has been proposed in which seawater is injected into a cultivation water tank, an aeration device is provided in the water tank, and a perforated basket containing sterile anaoasa is floated in the water tank. Sterile Anaanas in perforated cages are entangled by water currents and converge at one location.Also, as the Anaoa grows, the algal area increases, causing photoinhibition that reduces the amount of light transmitted through the culture tank. However, there has been a problem that the photosynthetic ability decreases and the culture speed decreases.

[0005]

DISCLOSURE OF THE INVENTION The present invention enables efficient growth of a sterile Anaaosa in a culture tank in a short period of time. Sterility that can efficiently cultivate Ana aurosa in the culture water tank without overlapping the alga bodies, reducing the amount of light transmission in the culture water tank, and causing no growth reduction due to light inhibition An object of the present invention is to provide a method for cultivating sex Anaaosa.

[0006]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and has an annular inner sleeve provided at a central portion of an annular water tank and having an opening in a peripheral surface, and provided in the annular inner sleeve. Using a culture water tank equipped with an overflow type drainage port and a water injection port that generates a rotating water flow along the inner peripheral surface of the annular water tank, cultivating Anaoasa while floating it in the rotating water flow of the culture water tank This is a method for cultivating sex Anaaosa

[0007] According to the present invention, the sterile Anaoa cultivated in the culturing water tank removes nutrients and CO ₂ from the seawater while floating along the inner circumference of the water tank by the rotating seawater flow supplied from the water inlet. It grows by absorbing from the algal surface. Also,
Seawater is drained from the inner sleeve opening to the drainage port in an overflow manner, and new seawater is supplied.The seawater in the water tank keeps a constant concentration gradient of nutrients etc., so it grows in the water tank. The growth speed is high, and there are no obstacles such as aeration pipes in the water channel of Ana-Aosa in the aquarium, so that the algae can be cultured without overlapping and causing light inhibition, and the growth of algae is promoted by the flow of seawater As a result, it is possible to culture Anaoasa effectively.

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The invention according to claim 1 of the present invention is directed to an annular inner sleeve provided at the center of an annular water tank and having an opening in a peripheral surface, and an overflow system provided in the annular inner sleeve. Using a culture water tank equipped with a drainage port and a water injection port that generates a rotating water flow along the inner peripheral surface of the annular water tank, culturing the sterile anaosa by floating it along the culture water tank with the rotating water flow of the culture water tank and wherein a sterile Ulva pertusa culture methods, Ulva pertusa is absorbed from nutrients and CO ₂ to Mosaku surface seawater while floating along the inner circumference of the water tank by the rotation flow of sea water supplied from water inlet Seawater in the aquarium is drained from the drainage outlet in an overflow manner through the opening in the inner sleeve, and fresh seawater is supplied.The seawater in the aquarium always has a constant concentration gradient of nutrients, etc. To The growth rate of algae is high, and the growth of algae is promoted by the flow of seawater, so that rapid cultivation is possible, and there are no obstacles such as aeration pipes in the water channel of Anaaosa in the aquarium. So, while floating, the algae overlap and do not cause light inhibition,
It has the effect that the culture can be efficiently performed while always maintaining a good culture state.

According to a second aspect of the present invention, there is provided the method for cultivating sterile Analeasa according to the first aspect, wherein the flow rate of the rotating water flow is 6 cm / sec. By doing so, it has the effect that the sterile Anaaosa can be cultured most efficiently.

According to a third aspect of the present invention, there is provided an annular inner sleeve provided at a central portion of an annular water tank and having an opening in a peripheral surface, an overflow drainage port provided in the inner sleeve, It is a culture water tank for sterile Anaanas that is equipped with a water inlet that generates a rotating water flow along the peripheral surface, and a culture water tank that can efficiently culture sterile Anaasa with a simple device. Has an action that can be provided.

[0011] The invention according to claim 4 is the invention according to claim 3.
Culture water tank and seafood breeding tank for CO._Twomixture
The tank is communicated with a closed flow path, and the culture water tank for Anaaosa is always
Sterile Anaoasa characterized by eutrophication
This is a cultivation system.
Eutrophication substances excreted from cultivation
Purification using sterile Ana-anasa_Two
Supply to the mixing tank, _TwoAdd Sterile Anaoasa
Increase the growth rate and increase the production capacity of sterile anaosa.
And has the effect of enabling.

An embodiment of the present invention will be described below with reference to the drawings. (Embodiment) FIG. 1 shows an Anaosa cultivation water tank of the present invention, 1 is an annular culture water tank, 2 is an annular outer wall of the culture water tank 1,
3 is an annular inner sleeve provided inside the annular outer wall 2,
4 is provided on the peripheral surface of the annular inner sleeve 3 and has a size such that only seawater is discharged and algal bodies do not flow out;
Reference numeral 5 denotes an overflow type drain port, and the lower end of the drain port 5 communicates with an external drain pipe through an opening 6 provided on the bottom surface of the water tank 1.

The height of the drain 5 defines the height of the water surface of the water tank 1 and is provided slightly below the upper end of the inner sleeve 3. Reference numeral 7 denotes a water injection port provided at the tip of the water injection pipe 7a to generate a rotating water flow (rectification) in a certain direction along the annular outer wall 2 of the water tank 1. Reference numeral 8 denotes an injection port provided at the outer periphery of the annular outer wall 2 of the water tank 1. A support frame to which the tip of the tube 7a is fixed,
It is adhered to the outer periphery of the outer wall 2.

A ball valve (not shown) is provided in the water injection pipe 7a to arbitrarily control the flow rate of the water flow injected into the water tank 1.

In the above embodiment, the water injection port is provided in the water tank 1.
Although two pieces are provided, two pieces may be provided above and below the water tank, and the number of pieces may be increased or decreased as necessary.

Hereinafter, the growth characteristics of the sterile Anaaosa by changing the flow rate using the culture water tank of the present invention were as follows.

As shown in FIG. 2, the relative growth rate (RGR%) of the sterile Ana-Aosa is greatly improved when the flow rate of the seawater in the culture tank is 3 cm / sec or more, and 6 cm / sec.
Showed the highest growth rate.

The reason for this is that Anaaosa absorbs nutrients and CO ₂ necessary for growth from the algal body surface, but when the water is stopped, the concentration gradient of nutrients and the like absorbed from the algal body surface decreases, and the flow rate becomes 3 cm. It is considered that the supply of seawater at a flow rate of / sec or more compensates for the decrease in the concentration gradient, so that a constant concentration gradient of nutrients and the like can be always maintained. As shown in FIG. 2, the maximum growth was observed at a seawater flow rate of 6 cm / sec.

Next, using a culture water tank having a diameter of about 30 cm and a capacity of 12.3 l, a culture test was carried out on sterile Anaoa at a flow rate of 6 cm / sec. The initial weight was 3 g as a sample.
f. w, 6 g. f. w, 9 g. f. w was used. As shown in FIG. 3, when the culture days reached 18 days, the wet weight of the algal cells was about 200 g. f. w,
In this state, the growth of Anaoasa decreases. The reason is that the algal wet weight is 200 g. f. It is considered that when the value reaches w, the shadow produced by the algae itself affects and photosynthesis is inhibited.

FIG. 4 shows the change in algal cell weight during the cultivation of Anaaosa in the test section of seawater flow of 6 cm / sec using the culture tank of the present invention and the conventional aeration test section.

The weight of the alga body of Anaoasa in the test plot of the present invention is almost the same as that in the aeration test plot up to 6 days of culture, but after 6 days of culture, the culture rate increases, and the culture rate increases. On the 18th day, the algal body weight was about 1.6 times that of the aeration test plot, and it was revealed that the culture method according to the present invention had a very high growth rate of sterile Anaoasa. .

Next, a fish and shellfish breeding system incorporating the culture tank of the present invention will be described.

FIG. 5 is an overall configuration diagram of the fish and shellfish breeding system of the present invention.

In the figure, reference numeral 1 denotes sterile Anaoa culture water
Tank, 5 is a drain pipe, 7a is a seawater injection pipe, 10 is a flow rate adjustment
Lube, 11 is a fish breeding tank, 12 is a seawater injection pipe, 13 is
CO _TwoMixing tank, 14 is CO_TwoSupply device, 15 is a circulation pump
And 16 is a supply pipe.

According to the present embodiment, natural seawater is supplied from the seawater injection pipe 12 to the fish breeding tub 11, and
Breeding of fish and shellfish. Although the concentration of the eutrophic substance (nitrogen, phosphorus, etc.) is increased in the breeding tank 11 due to the excretion from the fish and shellfish, the eutrophication substance-nutrient salts excreted from the fish and shellfish bred in the marine breeding tank 11 are increased. Purification is carried out using sterile anaosa which is cultivated in an anaosa culture tank 1, and the wastewater is supplied to a CO ₂ mixing tank 13 through a drain pipe 5a.
Since O ₂ is added and supplied from the fish breeding tank 11 to the culture tank 1, eutrophic substances and CO ₂
Because of the abundant supply of, it was possible to increase the growth rate and increase the production capacity of sterile anaosa.

[0026] The sterile anaoka alga bodies produced by the breeding system are used as feed for snails as they are, crushed,
By making it detritused, it becomes a feed for bivalves, so it also has the ability to produce food for seafood.

The sterile anaoasa produced by the small system simulating the breeding system was marked with 13C stable isotope, crushed, detritused and given to the pearl oyster. As shown in FIG. 6, the pearl oyster shell and soft body were obtained. There was no significant difference in the amount of carbon incorporated into the Pavlova luthori plankton diet similarly marked with 13 C stable isotopes. In addition, when the system was used for long-term breeding with the use of sterile anaosa as a closed system using marine fish as a closed system, the phosphorus and nitrogen concentrations of the breeding water are shown in FIGS. 7 (a) and 7 (b) with the purification device. like,
The value was kept to a low level, and it was concluded that the feed production and water purification type fish and shellfish breeding system was effective.

[0028]

According to the present invention, the sterile Anaoas cultivated in the culturing tank is fed by the seawater nutrients and CO while floating along the inner circumference of the tank by the rotating seawater flow supplied from the water inlet. ₂ absorbs from the algae surface and grows, and
Since seawater is drained from the inner sleeve opening to the drainage port in an overflow manner, a constant concentration gradient of nutrients etc. is maintained at all times, so the growth rate of algal cells is high and culture is performed by the flow of seawater To promote the growth of algae,
It is possible to more effectively culture sterile Anaphora. Also, since there is no obstacle such as an aeration pipe in the floating circuit of the anaosa in the water tank, the sterile anaosa can be always cultured under favorable growth conditions without overlapping of the algae and causing light inhibition.

[Brief description of the drawings]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 (a) is a side sectional view of a culture tank for sterile anaosa of the present invention, and FIG. 1 (b) is a plan view.

FIG. 2 is a graph showing the flow rate and the relative growth rate of sterile Anaokasa.

FIG. 3 is a graph showing the culture days and the algal wet weight.

FIG. 4 is a graph showing the number of days of culture and the algal cell weight by the method of the present invention and the conventional aeration method.

FIG. 5 is a schematic diagram showing a fish and shellfish breeding system of the present invention.

FIG. 6 is a graph showing breeding time and 13C isotope change.

FIGS. 7A and 7B are graphs showing breeding days and nitrogen and phosphorus concentrations.

[Explanation of symbols]

1 culture water tank 2 annular outer wall 3 annular inner sleeve 4 opening 5 discharge port 5a drain pipe 6 opening 7 water inlet 7a injection pipe 8 supporting frame 10 a flow rate adjusting valve 11 breeding tank 12 seawater injection pipe 13 CO ₂ mixing tank 14 CO ₂ supply device 15 Circulation pump 16 Supply pipe

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) C12M 3/02 C12M 3/02 C12N 5/04 C12N 5/02 5/02 (C12N 1/12 A // (C12N 1 / 12 C12R 1:89) C12R 1:89) (C12M 1/00 E (C12M 1/00 C12R 1:89) C12R 1:89) (C12M 1/02 A (C12M 1/02 C12R 1:89) C12R 1:89) (C12M 1/04 (C12M 1/04 C12R 1:89) C12R 1:89) (C12M 3/02 (C12M 3/02 C12R 1:89) C12R 1:89)) (C12N 5/04 (C12N 5/02 C12R 1:89) C12R 1:89) (C12N 5/02 C12N 5/00 F C12R 1:89) C12R 1:89) F term (reference) 2B026 AA05 AB08 AF04 4B029 AA02 BB04 CC01 DA03 DB17 DF08 DG06 4B065 AA13Y AA72X AA88X AC13 BC09 BC20 BC25 BD22 CA41

Claims (4)

[Claims] 1. An annular inner sleeve provided at a center portion of an annular water tank and having an opening in a peripheral surface, an overflow type drain port provided in the annular inner sleeve, and rotating along the inner peripheral surface of the annular water tank. A method for cultivating sterile Anaaosa, comprising culturing Anaoasa in a culture water tank having a water inlet for generating a water flow while floating Anaanasa in a rotating water flow of the culture water tank. 2. The method according to claim 1, wherein the flow rate of the rotating water flow is 6 cm / sec. 3. An annular inner sleeve provided at the center of the annular water tank and having an opening in the peripheral surface, an overflow type drain port provided in the inner sleeve, and a rotating water flow along the inner peripheral surface of the annular water tank. A culture tank for sterile Anaaosa, characterized by having a water inlet for generating a water. 4. The cultivation water tank for velvet as described in claim 3 is connected to a fish breeding cultivation tub and a CO ₂ mixing tub by a closed system flow path, and the cultivation cistern for ana aurosa is always in a eutrophic state. Sterile Anaaosa culture system.