JP2002020307A - Antioxidative composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidative composition characterized by improved water solubility, active oxygen eliminating ability, lipid peroxide controlling ability, and comprising an inorganic substance, a vitamin B group, a dietary fiber, phytic acid and a protein. SOLUTION: This antioxidative composition comprises a water-soluble rice bran extract as an active ingredient containing an inorganic substance, a vitamin B group, a dietary fiber, phytic acid and a protein in a rice bran and has active oxygen eliminating ability.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a flexible, safe, inorganic, vitamin B group, dietary fiber, phytic acid and protein which can be used in the fields of food, cosmetics and pharmaceuticals, for example. The present invention relates to a water-soluble acid-resistant composition derived from rice bran that can be simultaneously supplied.

[0002]

2. Description of the Related Art In recent years, it is said that at least part of the causes of lifestyle-related diseases such as myocardial infarction, arteriosclerosis, diabetes and cancer are due to lipid peroxide produced and accumulated in vivo. In order to solve such problems, antioxidants extracted from natural products and chemically synthesized antioxidants are known. For example, as a natural antioxidant, vitamin E or vitamin C, or SOD which is an enzyme in a living body
(Superoxide dismutase, active oxygen scavenging enzyme) and the like are used. As synthetic antioxidants, BHA (butylhydroxyanisole) and BHT
Phenol derivatives such as (butylhydroxytoluene) are known as antioxidants.

However, synthetic antioxidants have a problem in safety, and BHA is suspected to be carcinogenic. In the case of edible foods, the amount and range of use are often limited. Normally, many natural and synthetic antioxidants are fat-soluble. In the case of natural antioxidants, vitamin E and β-carotene have excellent lipid peroxide inhibiting ability, but their application range is limited because they are hardly soluble in water. There is a problem that is. Water-soluble natural antioxidants are limited and include, for example, vitamin C, glutathione, phytic acid, and SOD.
However, SOD is extremely expensive due to its difficulty in purification, and vitamin C and glutathione may act as pro-oxidants in the presence of metal ions, and depending on the conditions, may promote peroxidation of fats. There is. Further, the antioxidant ability of phytic acid is limited by the chelation of the transition metal, so that the range of use is limited.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide minerals, water-soluble vitamins and other micronutrients, dietary fiber, phytic acid and protein which are water-soluble, safe for the human body, and tend to be scarce for modern humans. It is an object of the present invention to provide an antioxidant composition which is more powerful than phytic acid and has an effect of suppressing lipid peroxide, which is excellent in flexibility and has active oxygen scavenging ability without phytic acid.

[0005]

SUMMARY OF THE INVENTION The present invention is an antioxidant composition comprising, as an active ingredient, a rice bran water-soluble extract containing minerals, water-soluble vitamins, dietary fiber and phytic acid in rice bran.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, in order to achieve the above object, when extracting from rice bran or defatted bran, an extract is obtained by using an organic acid, particularly phytic acid, contained in rice bran and dried. To produce a water-soluble rice bran extract powder. Here, water-soluble phytic acid is a component originally contained in rice bran, and there is no problem due to remaining in rice bran extract. Other organic acids, when using rice bran extract powder as food, may be any organic acid recognized as an optional food additive, for example, acetic acid, oxalic acid, tartaric acid, phosphoric acid, lactic acid, butyric acid, Citric acid, succinic acid and the like. When used in cosmetics, any organic acid that is recognized as an optional additive that can be used for this purpose may be used.
For example, citric anhydride, glacial acetic acid, lactic acid, succinic acid, citric acid, benzoic acid and the like can be mentioned. By specifying the type of the organic acid in this way, a step of removing these acids is not required, and the solubility of the dried powder in water can be maintained. In particular, when phytic acid is used for extraction, an extract obtained from pure rice bran can be obtained because the obtained extract contains no components other than those derived from rice bran.

[0007] If the pH at the time of extraction is about 5.5 or less, phytin can be extracted, and the object of the present invention can be achieved. , Preferably in the range of 4.5 to 5.5. About 30 minutes is sufficient for the extraction time. The extract is separated and recovered from the defatted bran by a method such as centrifugation. The resulting extract is a yellow-white opaque liquid.

Further, by drying the extract, a water-soluble rice bran extract powder can be obtained. As a drying method,
In order to suppress the denaturation of the rice bran extract component as much as possible, it is desirable to use a spray dryer or a freeze dryer. Also,
It is desirable to concentrate the extract in advance for efficient drying. The rice bran water-soluble extract used in the present invention is, for example, protein: 8.2 g / 100 g,
Dietary fiber: 11.1 g / 100 g, potassium: 5.33
g / 100 g, calcium: 121 mg / 100 g, magnesium: 2.65 g / 100 g, bioactive ingredient IP ₆ : 27.8 g / 100 g, vitamin B ₁ : 7.
19 mg / 100 g, vitamin B ₂ : 0.63 mg / 1
00g, vitamin _B 6: _10.2mg / 100g, iron:
19.1 mg / 100 g, zinc: 13.2 mg / 100
g, inositol: 11.1 g / 100 g.

The water-soluble rice bran extract thus obtained can be used as a conventional antioxidant or as a micronutrient, dietary fiber,
Depending on the intended use as an antioxidant with the supply of phytic acid and protein in mind, it is used in an appropriate form such as a powder or an aqueous solution. Flexible and safe antioxidants for food, food additives, cosmetics, external medicines, pharmaceuticals, etc., or
It may be added for antioxidant purposes with the supply of micronutrients, dietary fiber, phytic acid and protein in mind. For example, the addition amount of the water-soluble rice bran extract component in foods, food additives, cosmetics, external medicines and the like is usually 0.01 to 50.
% By weight, preferably about 0.1 to 2% by weight. The water-soluble rice bran extract has no toxicity and is also excellent in water solubility, so that no particular problem occurs even if it is added or taken in large amounts. Even when it is added as a supplement for minerals or water-soluble vitamins, it is not necessary to add another antioxidant, and since this component itself has antioxidant ability, the preservability of foods is improved. It can also be administered orally as a pharmaceutical or nutritional supplement. The dose in this case is not particularly limited, either, but for example, about 1 to 10 g / day is administered for the above-mentioned lifestyle-related diseases. Next, the present invention will be specifically described with reference to examples.

[0010]

Example: 200 liters of water was added to 20 kg of defatted bran,
Further, by adding a 50% phytic acid aqueous solution, the pH is adjusted to 5.
Adjusted to zero. After the mixture was stirred for 30 minutes to perform extraction, the extract was separated from the bran by a centrifuge. After concentrating the extract to 50 liters, it was dried using a spray dryer to obtain 5.3 kg of water-soluble rice bran extract powder. Table 1 shows the inorganic content of the water-soluble rice bran extract component.

[0011]

[Table 1]

As shown in Table 1, the water-soluble rice bran extract component is present in rice bran in addition to minerals derived from rice bran, vitamin B group, dietary fiber, and protein, and has anticancer activity and antioxidant activity. It contains a lot of phytic acid with useful activity. Hereinafter, test examples regarding these characteristics will be described.

Test Example 1 (1) Preparation of Rat Brain Homogenate Solution 9 ml of 0.075 M phosphate buffer (pH 7.5) was added to 1 g of frozen rat brain and homogenized under ice-cooling (hereinafter homogenate). liquid).

(2) Lipid Peroxide Inhibition Test To 0.5 ml of this homogenate, 3.5 ml of a 0.075 M phosphate buffer (pH 7.5) containing a water-soluble rice bran extract component was added, and the mixture was added at 37 ° C. for 2 hours. Incubated.
At this time, the final concentration of each water-soluble rice bran extract component was 0.01 to 0.2%. 0.5 ml each was collected immediately before the incubation and after 2 hours of incubation, and TBARS (Thiobarbituric r), which is an indicator of the amount of lipid peroxide, was obtained by the TBA (Thiobarbituric acid) method.
eactive substances) were measured. The antioxidant ability of the water-soluble rice bran extract component is determined by the TBARS amount when no water-soluble rice bran extract component is added and the TBARS amount when the water-soluble rice bran extract component is added.
It was calculated from the amount of BARS by the following formula 1 and expressed as the inhibition rate of increase in lipid peroxide due to incubation. In this test example and the test examples described below, the test was repeated three times, and the results were shown as the average of the obtained values.

Formula 1 Inhibition rate (%) = (1−A / B) × 100 A: T when a lipid peroxide inhibitor (antioxidant) is added
Increase value of BARS amount B: Increase value of TBARS amount without adding lipid peroxide inhibitor (antioxidant) TBA method 2.5 ml of 8% trichloroacetic acid solution and 0.67% thiobarbitur in 0.5 ml of sample Add 2.0 ml of acid solution,
After thorough mixing, the mixture was heated in a boiling aqueous solution for 15 minutes. Then, after centrifugation at 2000 × g for 10 minutes, the absorbance of the supernatant at 535 nm was measured, and TBA was measured.
The RS amount was determined. 1,1,3,3-tetraethoxypropane was used as a standard.

[0016]

[Table 2]

As shown in Table 2, as the concentration of the water-soluble rice bran extract component increases, the TBA value is suppressed, and the water-soluble rice bran extract component has an antioxidant activity despite containing many transition metals. Was confirmed to be present. The water-soluble rice bran extract component is considered to be a water-soluble antioxidant having transition metal resistance unlike vitamin C and glutathione.

Test Example 2 (1) Preparation of Rat Brain Homogenate Solution The same procedure as in Example 1 was performed.

(2) Lipid Peroxide Inhibition Test A 0.5 ml homogenate solution contains a water-soluble rice bran extract component, ascorbic acid (vitamin C) and sodium phytate so that the final concentration becomes 0.05%.
3.5 ml of 075M phosphate buffer (pH 7.5) was added, and the mixture was incubated at 37 ° C for 2 hours. After the incubation, the amount of TBARS was measured by the TBA method in the same manner as in Test Example 1, and the antioxidant ability of each additive was determined.

[0020]

[Table 3]

As shown in Table 3, it was confirmed that the antioxidant ability of the water-soluble rice bran extract component was stronger than that of phytic acid and comparable to that of vitamin C.

Test Example 3 (1) Preparation of Rat Brain Homogenate Solution The same procedure as in Example 1 was carried out.

(2) Lipid Peroxide Inhibition Test 0.5 ml of a 0.075 M phosphate buffer (pH 7.5) containing a water-soluble rice bran extract component was added to 0.5 ml of the homogenate solution.
and incubated at 37 ° C. for 2 hours. The final concentration of the water-soluble rice bran extract component was 0.01% or 0.1%. After the incubation, 0.5 ml was taken, and 2.5 ml of ethyl ether / ethanol (1: 3, v / v) was added thereto.
v) was added and shaken vigorously for 1 minute. Then 10
After centrifugation at 00 × g for 5 minutes, the absorbance at 234 nm of the upper layer was measured and used as an index of the amount of lipid peroxide (conjugated diene). Here, similarly to [Test Example 1], the antioxidant ability of the rice bran water-soluble extract was expressed as a lipid peroxide inhibition rate (%).

[0024]

[Table 4]

As shown in Table 4, even when conjugated diene was used as an indicator of lipid peroxide, the water-soluble rice bran extract showed high antioxidant ability.

Test Example 4 (1) Preparation of Linoleic Acid Solution To 50 mg of linoleic acid, 15 ml of 0.1 M phosphate buffer (pH 7.0) containing 0.25 g of Tween 20 was added, and the mixture was subjected to a polytron homogenizer for 1 minute. Homogenized (hereinafter linoleic acid solution).

(2) Lipid Peroxide Inhibition Test A 3.0 ml of a 0.1 M phosphate buffer (pH 7.0) containing a water-soluble rice bran extract component was added to 3.0 ml of this linoleic acid solution.
and incubated at 40 ° C. for 3 days with shaking in a loosely stoppered tube. At this time, the final concentration of the water-soluble rice bran extract component was 0.02% and 0.1%.
1%. 0.1 ml of each was collected immediately before the incubation and after 3 days of incubation, and the amount of peroxide was quantified by the thiocyanic acid method. The antioxidant capacity of the water-soluble rice bran extract component is calculated from the amount of peroxide when no water-soluble rice bran extract component is added and the amount of peroxide when the water-soluble rice bran extract component is added, using the following formula 1, and It was expressed as the inhibition rate of increase in oxidized lipid.

Formula 1: Inhibition rate (%) = (1−A / B) × 100 A: Increased amount of peroxide when a lipid peroxide inhibitor (antioxidant) is added B: Lipid peroxide inhibitor Increased amount of peroxide without adding (antioxidant) Thiocyanic acid method 0.1 ml of sample, 9.7 ml of 75% aqueous ethanol and 0.1 ml of 30% ammonium thiocyanate aqueous solution
Was added. 0.02M dissolved in 3.5% aqueous hydrochloric acid
0.1 ml of ferrous chloride solution is added and 5 minutes after exactly 3 minutes.
The absorbance at 00 nm was measured.

[0029]

[Table 5]

Test Examples 1 to 3 are examples using a biological component system, while Test Example 4 is an example using a system composed of a pure compound as an experimental system, and using lipid peroxide as an index for lipid peroxide. . Even when these different experimental systems or different indices were used, it was confirmed that the water-soluble rice bran extract had an antioxidant ability despite the high content of transition metals. It was shown that the presence of the antioxidant ability of the water-soluble rice bran extract component was more reliable and not a phenomenon observed only in a specific experimental system.

[Test Example 5] Radical scavenging test In this experiment, a phenoxy radical, which is a stable free radical, was used instead of the lipid hydroperoxy radical generally used in the experiment. This generally increases the accuracy of a radical measurement system that is easily affected by experimental conditions and the like to a system that can quantitatively measure the radical. Specifically, α, α-diphenyl-β-picrylhydrazyl (DPPH.) Is used. The measurement is based on the decrease in absorbance at 517 nm of the sample accompanying the decrease in DPPH.

DPPH method The water-soluble rice bran extract was dissolved in 100% ethanol at a final concentration of 0.01 to 0.1%, and sodium phytate was dissolved in 100% ethanol at a concentration of 0.1%. And
To 2 ml of 0.1 M acetate buffer (pH 5.5), 2 ml of the above-mentioned ethanol solution of the test substance was added. 0.
1 ml of a 5 mM DPPH / ethanol solution was added to make the total amount 5 ml. Immediately thereafter, the absorbance at 517 nm was measured. After 30 minutes, the absorbance at 517 nm was measured again. As a control, the same operation was performed using only ethanol as a solvent instead of a sample in which a test substance was dissolved in ethanol. The radical scavenging ability of the test substance was evaluated by the radical scavenging rate (A) calculated by the following equation.

A = {(absorbance of control at 0 min) − (absorbance of test substance at 30 min)} / (absorbance of control at 30 min) × 100

[0034]

[Table 6]

Test Example 5 shows that the water-soluble rice bran extract component has a radical scavenging ability, and one of the mechanisms of the antioxidant ability of the water-soluble rice bran extract component is related to the radical scavenging ability. . Furthermore, since phytic acid, which is known to have antioxidant ability, did not have radical scavenging ability, the radical scavenging ability of the water-soluble rice bran extract component is not due to phytic acid, It was shown that the components other than phytic acid were deeply involved in the antioxidant ability of the rice bran extract components.

[0036] [Test Example 6] superoxide (O ₂ ^-) erase test water soluble rice bran extract component of O ₂ ^- The scavenging activity was examined by nitroblue tetrazolium (NBT) reduction method. This method is a method utilizing the fact that O ₂ ⁻ generated when xanthine oxidase is allowed to act on hypoxanthine reduces NBT to form dark blue formazan having an absorption at 560 nm. This system O ₂ ^- When a substance having a scavenging ability is present, the amount of formazan generated by reducing obtains a formazan product inhibition rate from formazan product amount when the active ingredient added for formazan product amount when the active ingredient without additives , O ₂ ^- was the erasure rate.

Nitroblue tetrazolium (NBT) reduction method Water-soluble rice bran extract has a final concentration of 0.05 to 1.0%.
So that 0.05M sodium carbonate buffer (pH
10.2) to obtain a sample. SOD made by Wako Pure Chemical
-Measured using a Test Wako. As a control, a 0.05 M sodium carbonate buffer (pH 10.2) containing no water-soluble rice bran extract component was used. O soluble rice bran extract component from the following equation ₂ ^- was calculated erasure rate.

O ₂ ^~ Elimination rate = (BA) / B × 100 A: Absorption at 560 nm due to formazan formation when water-soluble rice bran extract is added B: Formazan formation when no water-soluble rice bran extract is added Absorption at 560 nm

[0039]

[Table 7]

As shown in Table 7, Test Example 6 shows that the water-soluble rice bran extract component has an active oxygen scavenging ability, and one of the mechanisms of the antioxidant ability of the water-soluble rice bran extract component is active. It became clear that it was due to oxygen scavenging ability.

Next, the compositions for various uses (foods, beverages, cosmetics, feeds, etc.) containing the composition of the present invention will be exemplified below. Here, RICEO, RICEO-EX
Is the trade name of the composition according to the present invention.

[0042]

[Table 8]

[0043]

[Table 9]

[0044]

[Table 10]

[0045]

[Table 11]

[0046]

[Table 12]

[0047]

[Table 13]

[0048]

[Table 14]

[0049]

[Table 15]

[0050]

[Table 16]

[0051]

[Table 17]

[0052]

[Table 18]

[0053]

[Table 19]

[0054]

[Table 20]

[0055]

[Table 21]

[0056]

[Table 22]

[0057]

[Table 23]

[0058]

[Table 24]

[0059]

The antioxidant composition of the present invention is water-soluble, so that it can be used for products having a low fat content. Furthermore, since it is derived from natural rice bran, it is safe and inexpensive. In addition, it contains phytic acid, which is known to have antioxidant ability, but has stronger antioxidant ability than phytic acid itself, and also has active oxygen scavenging ability not found in phytic acid. Further, since many transition metals are present in the composition itself, it is considered that the composition is an antioxidant composition which is not affected by transition metals in the environment. Minerals derived from rice bran,
Since it contains the vitamin B group, dietary fiber, phytic acid and protein, it is considered that it is also effective as a nutritional supplement at the same time. Specifically, the antioxidant composition according to the present invention can be used in the cosmetics field (lotion cream, toiletries, etc.) and the food field (tablets, powdered drinks, liquid drinks, sports drinks, pickles, cans, dressings, mayonnaise, confectionery, etc.) ), Feed, medicine, etc.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61K 7/06 A61K 7/06 7/075 7/075 7/50 7/50 A61P 39/06 A61P 39/06 F-term (Reference) 4B018 MD49 ME06 MF01 4C083 AA082 AA111 AA112 AA122 AB032 AB242 AB292 AB312 AB352 AB442 AC012 AC022 AC072 AC102 AC122 AC132 AC182 AC242 AC352 AC402 AC422 AC432 AC442 AC472 AC482 AC532 AC542 AC712 AC782 AC842 AC902 AD092 AD152 AD532 AD332 CC05 CC12 CC22 CC23 CC25 CC37 CC38 DD17 DD23 DD31 EE10 EE12 4C088 AB74 AC04 BA08 CA03 NA14 ZC80

Claims (5)

[Claims] 1. An antioxidant composition comprising a water-soluble rice bran extract containing an inorganic substance, a water-soluble vitamin, dietary fiber, phytic acid and protein as an active ingredient, and having an ability to scavenge active oxygen. 2. The antioxidant composition according to claim 1, wherein the water-soluble extract of rice bran is a water-soluble extract of rice bran extracted from natural rice bran. 3. The extract according to claim 1, wherein the extract comprises an organic acid, acetic acid, oxalic acid, tartaric acid, phosphoric acid, lactic acid contained in rice bran.
An antioxidant composition which is extracted with one or more selected from the group of butyric acid, citric acid and succinic acid. 4. The extract according to claim 1, wherein the extract comprises an organic acid, citric anhydride, glacial acetic acid, lactic acid, succinic acid contained in rice bran.
An antioxidant composition which is extracted with one or more selected from the group consisting of citric acid and benzoic acid. 5. An antioxidant composition, wherein the extract according to claim 1 is extracted with an extract containing water-soluble phytic acid.