JP2007223990A - Antioxidant composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a nonconventional antioxidant composition involved in aggravation of diseases and aging prevention of the skin, health maintenance, or prevention of deterioration in quality of fats and oils, etc., and from unpolished rice, polished rice and rice bran each containing a functional component in abundance. <P>SOLUTION: The present inventors obtained a nonconventional, completely new and excellent antioxidant composition by extracting unpolished rice, polished rice or rice bran containing a functional component in abundance with subcritical ethanol or supercritical ethanol. The applications of the above antioxidant are not limited to only foods, but cover wide variety of products such as medicines, fertilizers, feed and chemicals. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an antioxidant composition comprising a component extracted from brown rice, white rice and rice bran using subcritical ethanol or supercritical ethanol.

  In recent years, it is said that at least a part of the causes of lifestyle-related diseases such as myocardial infarction, arteriosclerosis, diabetes and cancer is due to lipid peroxide generated and accumulated in vivo. Antioxidants extracted from natural products and chemically synthesized antioxidants are known as solutions for solving such problems. Antioxidants made from natural products or chemically synthesized are widely used for the purpose of preventing the deterioration of the quality of fats and oils including edible salad oil. Antioxidants are used not only for foods but also for pharmaceuticals and cosmetics.・ A wide variety of products such as fertilizer, feed, and chemical products.

  In recent years, many antioxidant components extracted and purified from animals and plants have been found, and components having antioxidant ability such as phytic acid and ferulic acid are also known in rice and rice bran. Moreover, the antioxidant composition as a mixing component as described in Patent Documents 1 and 2 is also known.

JP 2002-020307 A On the other hand, when ethanol is put in a sealed container and heated, the density of the gas increases, the density of the gas and the liquid eventually becomes equal, and the boundary disappears. This state is called supercritical, this fluid is called supercritical ethanol, and the point at which the boundary disappears (temperature 243 ° C., pressure 6.1 MPa) is called the critical point. In a region close to the critical point, for example, 200 ° C. and 12 MPa, ethanol is in a liquid state, and this state of ethanol is called subcritical ethanol. A variety of reactions occur in sub-supercritical ethanol, including decomposition of substances (hydrolysis and thermal decomposition), oxidation (in the presence of an oxidizing agent), recombination, and polymerization. Can be prioritized.

  Attempts to act on animal and plant materials using the properties of supercritical ethanol or subcritical ethanol have been widely used in the past, and are used for flavor extraction, organic waste processing, and the like (for example, Patent Document 3). 4). However, these attempts are limited to processing for the purpose of reducing and treating waste and for the purpose of making it easy to ingest existing functional substances such as dietary fiber and minerals. No attempt has been made to obtain new functional substances by causing these fluids to act on rice bran.

Japanese Patent Laying-Open No. 2005-081332 JP 2005-161159 A

  This invention is made | formed in view of the said subject, The objective aims at obtaining the antioxidant composition which has not existed conventionally from brown rice, white rice, and rice bran.

  As a result of earnest research to solve the above problems, the present inventors have found that the components extracted from brown rice, white rice and rice bran using subcritical ethanol or supercritical ethanol have an excellent antioxidant effect, An entirely new antioxidant composition that has never been obtained has been obtained.

  According to this invention, it has become possible to provide an antioxidant composition derived from rice, which has an excellent antioxidant action, from rice containing abundant functional components.

  In the present invention, brown rice and white rice used as extraction raw materials are preferably pulverized using a pulverizer or the like. The rice bran refers to a regular rice bran obtained by whitening brown rice, a defatted pork defatted by an organic solvent or pressing, etc., and a rice bran after extracting components such as vitamin B group and protein. The varieties, growing seasons, regions, etc. are not particularly limited.

  For supercritical ethanol and subcritical ethanol used for extraction, in addition to using commonly used supercritical ethanol production equipment and supercritical decomposition equipment, it is possible to generate supercritical ethanol and subcritical ethanol states in autoclave equipment, etc. If it is a thing, it will not specifically limit.

  Water is added to the extraction raw material and extracted for a certain period of time under heating and pressurization to a supercritical or subcritical state, and then cooled.

  The extract thus prepared may be used as an antioxidant as it is, but it is preferable to further carry out treatments such as purification, concentration and drying.

  Examples of the concentration and purification treatment include filtration, membrane concentration, column purification and the like, but are not particularly limited, and the drying method may be any commonly used drying method.

  The amount of the antioxidant composition obtained according to the present invention is not particularly limited, but is usually about 10 ppm to 10%.

  Other additives such as dextrin and emulsifier may be appropriately blended in the antioxidant composition obtained in the present invention depending on the intended use.

  Antioxidant compositions obtained in this way can be added to oils and other foods, pharmaceuticals, cosmetics, fertilizers, feeds, chemicals, etc., to prevent quality deterioration or prevent illness, skin aging, and health. Contributes to improvement.

  Furthermore, the anti-oxidant composition obtained in the present invention can be expected to have a synergistic effect for further enhancing the anti-oxidation effect by using it together with an existing anti-oxidant composition such as vitamin E.

  Hereinafter, embodiments of the present invention will be described in detail based on examples. However, the present invention is not limited to the following examples.

70 g of ethanol is added to 10 g of deproteinized and defatted rice bran, placed in a reaction vessel of an autoclave apparatus (manufactured by Nitto High Pressure Co., Ltd.), sealed, and then heated while stirring. After reaching 195 ° C., the temperature is maintained for 24 hours and then cooled, and the extract and the residue are removed from the reaction vessel. No. The mixture was filtered through 5B filter paper, and the obtained filtrate was freeze-dried to obtain 4.6 g of a light brown subcritical ethanol extract.
(Comparative Example 1)
Ethanol is refluxed in 10 g of deproteinized and defatted rice bran and stirred for 24 hours. Remove the extract and residue from the reaction vessel. After removing the residue by centrifugation (3,000 rpm, 5 min), The mixture was filtered through 5C filter paper, and the resulting filtrate was lyophilized to obtain 0.1 g of a light brown subcritical ethanol extract.

  When Example 1 and Comparative Example 1 are compared, it can be seen that the present invention can obtain an ethanol extract of deproteinized and defatted rice bran in high yield. Examples relating to these antioxidant properties will be described below.

  The DPPH radical scavenging activity ability of the extract prepared in Example 1 was measured. The result is shown in FIG. From the figure, it was confirmed that this extract contains a plurality of DPPH radical scavenging active ingredients. The specific method is shown below.

  Take the sample in the capillary and spot the sample on the TLC. Next, TLC is placed in a developing tank containing a developing solution (hexane: ethyl acetate = 2: 1) and developed. When the solvent is near the top of the TLC, remove the TLC and stop developing. When the solvent is dry, spray the color former (2 mg / mL DPPH 95% ethanol solution) in a fume hood to a wet level. DPPH radical scavenging activity is possessed by the spot material where the dye has faded.

It is drawing which shows the radical scavenging ability of the extract concerning this invention, and the component of the spot which is black has an antioxidant effect | action.

Claims (6)

  An antioxidant composition comprising components obtained by extraction from brown rice, white rice and rice bran using subcritical ethanol.   An antioxidant composition characterized by containing components obtained by extraction from brown rice, white rice and rice bran using supercritical ethanol   An oil or fat preservative comprising the antioxidant composition according to claim 1 or 2.   A composition that enhances the antioxidant effect by blending the antioxidant composition according to claim 1 or 2 and a conventional antioxidant composition.   Pharmaceuticals, cosmetics, foods, feeds, and fertilizers characterized in that they contain the antioxidant composition according to claim 1 or 2. A chemical product comprising the antioxidant composition according to claim 1 or 2.

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