JP2001299305A - Composition for scavenging active oxygen, and method for producing the same - Google Patents
Composition for scavenging active oxygen, and method for producing the sameInfo
- Publication number
- JP2001299305A JP2001299305A JP2000159136A JP2000159136A JP2001299305A JP 2001299305 A JP2001299305 A JP 2001299305A JP 2000159136 A JP2000159136 A JP 2000159136A JP 2000159136 A JP2000159136 A JP 2000159136A JP 2001299305 A JP2001299305 A JP 2001299305A
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- active oxygen
- composition
- extract
- plant
- eliminating
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- Anti-Oxidant Or Stabilizer Compositions (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、特定の原材料を用
いてなる活性酸素消去用組成物、その製造法及び利用に
係るものである。より詳しくは、アブラナ科植物の植物
体の水及び／又は親水性有機溶媒による抽出エキスを含
有してなる活性酸素消去用組成物に関するものであり、
また、該組成物の製造法および利用に関するものであ
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for erasing active oxygen using a specific raw material, a method for producing the same, and a use thereof. More specifically, the present invention relates to a composition for eliminating active oxygen, comprising an extract of water and / or a hydrophilic organic solvent extracted from a plant of a cruciferous plant,
The invention also relates to a method for producing and using the composition.
【０００２】[0002]
【従来の技術】ヒトをはじめとする好気的生物のエネル
ギーは主に生体内での酸化的リン酸化反応に基づくもの
であり、その生命維持には大量の酸素を必要とする。ヒ
ト成人では日常生活の維持のために、１日約５００Ｌの
酸素を消費している。酸素消費量は、臓器、細胞及び固
体の状態により著しく異なるが、生体内に取り込まれた
酸素の数％は、常に種々の酵素代謝系によりスーパーオ
キサイドアニオン、過酸化水素、ヒドロキシラジカル、
一重項酸素、アルコキシラジカル等の活性化された酸素
（以下、活性酸素という）に変化している。それらの分
子種の多くは反応性が高く、生体内で産生された活性酸
素は、体内に侵入する微生物等に対して殺菌作用を示
し、生体への感染を防いでいる。2. Description of the Related Art Energy of aerobic organisms including humans is mainly based on oxidative phosphorylation in vivo, and a large amount of oxygen is required for maintaining the life. Adult humans consume about 500 L of oxygen a day to maintain daily life. Oxygen consumption varies significantly depending on the state of organs, cells, and solids, but several percent of oxygen taken into the body is constantly superoxide anion, hydrogen peroxide, hydroxyl radical,
It has been changed to activated oxygen such as singlet oxygen and alkoxy radical (hereinafter referred to as active oxygen). Many of these molecular species have high reactivity, and active oxygen produced in a living body has a bactericidal action against microorganisms and the like that invade the body, thereby preventing infection of the living body.
【０００３】一方、活性酸素は生体内で脂質、タンパク
質、核酸、糖質などを攻撃し、その機能を障害すること
が知られている。すなわち、ラジカル連鎖反応により、
生体機能を障害する反応産物を増加させ、様々な疾病を
発症させ、また、病態を悪化させる危険性を有してい
る。例えば、酵素の失活、過酸化脂質の生成、ＤＮＡ鎖
の切断、赤血球膜やミトコンドリア膜等の破壊作用が起
こり、また、それらの作用により心筋梗塞、動脈硬化、
癌、糖尿病、肝臓障害、脳卒中、白内障、肩こり、冷え
性、しみ、そばかす、しわ等が生じる。[0003] On the other hand, it is known that active oxygen attacks lipids, proteins, nucleic acids, carbohydrates, and the like in vivo and impairs their functions. That is, by the radical chain reaction,
There is a risk of increasing reaction products that impair biological functions, causing various diseases, and worsening the condition. For example, inactivation of enzymes, production of lipid peroxides, breakage of DNA chains, destruction of erythrocyte membranes, mitochondrial membranes, etc. occur, and these actions cause myocardial infarction, arteriosclerosis,
Cancer, diabetes, liver damage, stroke, cataract, stiff shoulders, chills, spots, freckles, wrinkles, etc. occur.
【０００４】したがって、生命維持には生体内代謝で発
生する活性酸素を、効率よく分解処理することが重要で
ある。通常、生物は生体内でスーパーオキシドジスムタ
ーゼ、カタラーゼ、グルタチオンペルオキシダーゼ等の
酵素により、活性酸素から自己を守る抗酸化防御システ
ムを有している。しかし、ストレスや高齢化等によりそ
れらの酵素量が減少すると活性酸素が過剰に蓄積され、
これにより生体内バランスが崩れ、前述のような病態を
発症または悪化させてしまう。そこで、生成された過剰
な活性酸素を消去するには、安全で副作用のない食品、
食品素材中に含まれる活性酸素消去機能のある物質を補
給することが必要となってくる。[0004] Therefore, it is important to efficiently decompose active oxygen generated by metabolism in a living body to maintain life. In general, living organisms have an antioxidant defense system that protects themselves from active oxygen by enzymes such as superoxide dismutase, catalase, and glutathione peroxidase in vivo. However, when the amount of those enzymes decreases due to stress or aging, active oxygen is excessively accumulated,
As a result, the balance in the living body is lost, and the above-mentioned pathological condition is developed or worsened. Therefore, in order to eliminate the excess active oxygen generated, safe and side-effect free foods,
It is necessary to replenish substances having an active oxygen eliminating function contained in food materials.
【０００５】活性酸素を消去する作用のある物質の探索
が従来から行われてきた。例えば、生体内でも生成され
るスーパーオキシドジスムターゼがあるが、これは蛋白
質であり、経口摂取では消化されたり、注射投与でも血
中残存性が低く実用には適さない。また、アスコルビン
酸（ビタミンＣ）やトコフェロール（ビタミンＥ）等の
いわゆる抗酸化性物質が酸化防止の点から用いられてい
るが、活性酸素消去機能の点ではいずれも効果が小さ
く、安定性の面でも難点がある。このほか、天然物とり
わけ生薬原料から抽出された活性酸素消去成分が提案さ
れており、例えば特開昭６１−２４５２２号、特開平２
−１９３９３０号、特開平２−２６４７２７号、特開平
３−１５３６２９号、特開平４−６９３４３号、特開平
４−２０２１３８号等の各公報に記載の発明がある。し
かし、これらは活性酸素を消去する効果の点から十分に
満足できるものではなく、実用的ではなかった。[0005] Searching for a substance having a function of eliminating active oxygen has been conventionally performed. For example, superoxide dismutase, which is produced in vivo, is a protein, which is digested by ingestion or has low persistence in blood by injection, making it unsuitable for practical use. Also, so-called antioxidants such as ascorbic acid (vitamin C) and tocopherol (vitamin E) are used from the viewpoint of antioxidation, but they have little effect in terms of active oxygen scavenging function and are not stable. But there are drawbacks. In addition, active oxygen-scavenging components extracted from natural products, particularly crude drug materials, have been proposed.
JP-A-193930, JP-A-2-264727, JP-A-3-153629, JP-A-4-69343, JP-A-4-202138 and the like. However, these are not sufficiently satisfactory in terms of the effect of eliminating active oxygen, and are not practical.
【０００６】一般に、天然物である植物類にはフラボノ
イド、タンニン、カテキン、ポリフェノール等の成分が
含まれており、これらは活性酸素を消去する機能がある
ことが知られている。例えば、ブドウ種子から水や親水
性有機溶媒を用いて抽出されるエキスにはポリフェノー
ル類が含まれ、とりわけアントシアニジン、プロアント
シアニジン等が多く含まれており、活性酸素消去能や抗
酸化能のある食品素材として市販されている。また、フ
ラボノイドやカテキンがアセトアルデヒドや過酸化水素
等の存在下において活性酸素を消去する作用を示すとき
に微弱な発光現象が認められ、この相関性から活性酸素
消去能のある成分や素材を探索しようとする試みがある
（Ｙ．Ｙｏｓｈｉｋｉ ｅｔ ａｌ．，Ｐｈｙｔｏｃｈ
ｅｍｉｓｔｒｙ，３９，２２５−２２９（１９９５）、
同Ｊ．Ｂｉｏｌｕｍｉｎ．Ｃｈｅｍｉｌｕｍｉｎ．，１
０，３３５−３３８（１９９５）等）。このように活性
酸素消去能を有する素材の開発は鋭意検討されている
が、これらを商業ベースの製品に応用しても、実際には
所望の効果を十分に発現し得るものであるとはいえず、
より強力かつ効果的に活性酸素を消去するものが求めら
れていた。In general, plants that are natural products contain components such as flavonoids, tannins, catechins and polyphenols, and these are known to have a function of eliminating active oxygen. For example, extracts extracted from grape seeds using water or a hydrophilic organic solvent contain polyphenols, especially anthocyanidins, proanthocyanidins, etc., and are rich in active oxygen scavenging ability and antioxidant foods. It is commercially available as a material. In addition, when flavonoids and catechins show the action of scavenging active oxygen in the presence of acetaldehyde, hydrogen peroxide, etc., a faint light emission phenomenon is observed. Based on this correlation, search for components and materials capable of scavenging active oxygen. (Y. Yoshiki et al., Phytoch)
chemistry, 39 , 225-229 (1995),
J. Biolumin. Chemilumin. , 1
0 , 335-338 (1995)). Although the development of materials having the ability to scavenge active oxygen has been intensively studied, even if these materials are applied to commercial products, the desired effects can actually be sufficiently exhibited. Without
There is a need for a more powerful and effective scavenger of active oxygen.
【０００７】[0007]
【発明が解決しようとする課題】かかる現状に鑑み、本
発明では、生体のＤＮＡ、細胞、組織等に酸化的損傷や
傷害を与え、老化症状や各種疾病をひきおこすと考えら
れている活性酸素を強力かつ効果的に消去ないしは除去
でき、安全性の点で懸念のない活性酸素消去用組成物及
びその製造法を提供し、さらには該組成物を配合した食
用組成物を提供することを目的とした。SUMMARY OF THE INVENTION In view of the above-mentioned situation, according to the present invention, active oxygen, which is thought to cause oxidative damage or damage to DNA, cells, tissues, etc. in living organisms and cause aging symptoms and various diseases, is provided. It is an object of the present invention to provide a composition for erasing active oxygen which can be strongly or effectively erased or removed and has no safety concerns and a method for producing the same, and further to provide an edible composition containing the composition. did.
【０００８】[0008]
【課題を解決するための手段】前記課題の活性酸素消去
用組成物は、アブラナ科植物の植物体の抽出物を含有し
てなる活性酸素消去用組成物によって達成される。ここ
で、アブラナ科植物としてはケール、ハボタン、ブロッ
コリー及びカリフラワーからなる群から選ばれる１種又
は２種以上のものが望ましく、このうちケールが特に好
ましい。また、本発明に係る抽出物は、アブラナ科植物
を水及び／又は親水性有機溶媒を用いて抽出処理して得
られる粉末状エキスであることが望ましく、前記の抽出
溶媒は酸性を呈するものがさらに望ましい。なお、抽出
時の温度は０〜１００℃、より好ましくは５０〜１００
℃である。さらには、本発明に係る抽出物は沈殿分離や
イオン交換カラム等の処理に供して精製し、とくに陰イ
オン交換処理を経て精製されたものがより一層望まし
い。Means for Solving the Problems The active oxygen scavenging composition of the object is achieved by an active oxygen scavenging composition containing an extract of a plant of the Brassicaceae plant. Here, the cruciferous plant is desirably one or more selected from the group consisting of kale, havot, broccoli, and cauliflower, and kale is particularly preferred. Further, the extract according to the present invention is preferably a powdery extract obtained by extracting a cruciferous plant with water and / or a hydrophilic organic solvent, and the above-mentioned extraction solvent is preferably acidic. More desirable. The temperature at the time of extraction is 0 to 100 ° C, more preferably 50 to 100 ° C.
° C. Furthermore, the extract according to the present invention is subjected to purification by precipitation separation or treatment with an ion exchange column or the like, and it is more preferable that the extract is purified through anion exchange treatment.
【０００９】本発明の活性酸素消去用組成物は、また、
前記のアブラナ科植物の植物体の抽出物と、活性酸素消
去能を有する公知の素材とを少なくとも含有してなる活
性酸素消去用組成物によっても達成される。ここで、活
性酸素消去能を有する公知の素材として、アスコルビン
酸（ビタミンＣ）、トコフェロール（ビタミンＥ）、カ
テキン類、アントシアニン類、フラボノイド類、これら
以外のポリフェノール類、緑茶エキス、ほうじ茶エキス
及びルイボスティーエキスからなる群から選ばれる少な
くとも１種であることが望ましい。The composition for scavenging active oxygen of the present invention further comprises:
The present invention is also achieved by an active oxygen eliminating composition comprising at least an extract of a plant of the above Brassicaceae plant and a known material having an active oxygen eliminating ability. Here, as a known material having active oxygen scavenging ability, ascorbic acid (vitamin C), tocopherol (vitamin E), catechins, anthocyanins, flavonoids, other polyphenols, green tea extract, roasted tea extract and rooibos tea Desirably, it is at least one selected from the group consisting of extracts.
【００１０】前記課題の活性酸素消去用組成物の製造法
は、アブラナ科植物の植物体を生のまま又は乾燥させチ
ップ状に切断したものを水及び／又は親水性有機溶媒を
用いて０〜１００℃で抽出し、該抽出液から溶媒を除去
する方法によって達成される。ここで、アブラナ科植物
はケールであり、この乾燥物の切断物を原料として用い
ることが好ましい。また、抽出の溶媒は酸性とし、温度
は５０〜１００℃、抽出時間は０．５〜５０時間である
ことが望ましい。さらに、より顕著な効果を奏する活性
酸素消去用組成物の製造法としては、前述の製造法によ
って得られた抽出物に対して、沈殿分離やイオン交換処
理等の精製処理、より望ましくは陰イオン交換処理を施
すことにより達成される。[0010] The method for producing a composition for eliminating active oxygen according to the above-mentioned subject is characterized in that a plant of a Brassicaceae plant is cut raw and dried and cut into chips using water and / or a hydrophilic organic solvent. This is achieved by extracting at 100 ° C. and removing the solvent from the extract. Here, the cruciferous plant is kale, and it is preferable to use a cut product of the dried product as a raw material. Further, it is desirable that the extraction solvent is acidic, the temperature is 50 to 100 ° C., and the extraction time is 0.5 to 50 hours. Further, as a method for producing the active oxygen-scavenging composition exhibiting a more remarkable effect, the extract obtained by the above-mentioned production method is subjected to purification treatment such as precipitation separation or ion exchange treatment, and more desirably anion. This is achieved by performing an exchange process.
【００１１】さらに、前記課題の食用組成物は、前述の
いずれかの活性酸素消去用組成物を配合してなる食用組
成物によって達成される。Further, the edible composition of the object is achieved by an edible composition comprising any one of the aforementioned active oxygen eliminating compositions.
【００１２】[0012]
【発明の実施の形態】まず、本発明の活性酸素消去用組
成物について以下にさらに詳述する。本発明の活性酸素
消去用組成物は、アブラナ科植物の植物体の抽出物を含
有してなるものである。DESCRIPTION OF THE PREFERRED EMBODIMENTS First, the active oxygen scavenging composition of the present invention will be described in more detail below. The composition for eliminating active oxygen of the present invention contains an extract of a plant of the Brassicaceae plant.
【００１３】原料として用いるアブラナ科植物は、具体
例としてケール（Ｂｒａｓｓｉｃａｏｌｅｒａｃｅａ
Ｖａｒ．ａｃｅｐｈａｌａ）（キッチンケール、ツリー
ケール、ブッシュケール、マローケール、コラード、緑
葉カンラン等）、ハボタン、ブロッコリー、カリフラワ
ー、アブラナ、ハクサイ、キャベツ、メキャベツ（コモ
チカンラン）、小松菜、チンゲンサイ、カラシナ、コー
ルラビ、クレソン（オランダガラシ）、タアサイ、カ
ブ、大根、ワサビ、キョウナ、ガーデンクレス、ロケッ
ト、マスタード、ナズナ、ハタザオ、コンロンソウ等を
あげることができ、これらのうちケール、ハボタン、ブ
ロッコリー及びカリフラワーからなる群から選択される
１種又は２種以上を用いることが望ましい。最も望まし
いものはケールである。使用する植物体の部位はとくに
限定されるものではないが、葉部などの通常食用に供す
る部分が好ましい。なお、原料形態は生のまま使用して
もよいが、より好ましくは乾燥したものを適度な大きさ
に切断して用いる。The cruciferous plant used as a raw material is, for example, kale (Brassica oleracea).
Var. acephala) (kitchen kale, tree kale, bush kale, mallow kale, collard, green leaf orchid, etc.), habotan, broccoli, cauliflower, rape, Chinese cabbage, cabbage, meka cabbage (komochikan orchid), komatsuna, chingensai, mustard, kohlrabi, watercress ), Taasai, Turnip, Radish, Wasabi, Kyona, Garden Cress, Rocket, Mustard, Nazuna, Hatazao, Hakone, etc. Among them, one selected from the group consisting of kale, habotan, broccoli and cauliflower Alternatively, it is desirable to use two or more kinds. Most preferred is kale. The site of the plant to be used is not particularly limited, but a portion that is normally used for food, such as a leaf, is preferable. The raw material may be used as it is, but more preferably, it is dried and cut into a suitable size.
【００１４】本発明の活性酸素消去用組成物に係る抽出
物は次のようにして得ることができる。すなわち、前記
原料に対して３〜１５重量倍の水及び／又は親水性有機
溶媒を加え、温度０〜１００℃、より好ましくは５０〜
１００℃で０．５〜５０時間、１回もしくは繰り返し抽
出する。ついで、抽出残渣を濾別や遠心分離により除去
して抽出液を得、必要に応じて減圧下で濃縮処理を施
し、さらには噴霧乾燥あるいは凍結乾燥等の処理によっ
て水分を除去して活性酸素消去能に優れた粉末状の抽出
物を調製する。親水性有機溶媒としては、例えば、メタ
ノール、エタノール、プロパノール、ブタノール、アセ
トン、アセトニトリル、酢酸、ギ酸等を使用することが
できるが、これらを高濃度のままで使用すると、得られ
る抽出物の活性酸素消去能は低下する。したがって、前
記の親水性有機溶媒を含水させ、メタノールやエタノー
ルの場合は３０容量％以上の含水率、その他の溶媒の場
合は、５０容量％以上の含水率にすることが望ましい。
また、抽水用溶媒として塩酸、リン酸、酢酸等を用いて
酸性状態、すなわちｐＨ７未満、より好ましくはｐＨ６
〜２にしたものを用いると、活性酸素消去能がより高い
抽出物を得ることができる。なお、抽出液から溶媒を除
去するにあたっては、本発明に係る抽出物成分は熱に対
して比較的安定であり、凍結乾燥処理に限定されるもの
ではない。しかしながら、吸湿による変質や酸化による
本発明の所望の効果の低下が起こる可能性があり、空気
との接触頻度を可及的に少なくすることが望ましい。The extract according to the active oxygen eliminating composition of the present invention can be obtained as follows. That is, 3 to 15 times by weight of water and / or a hydrophilic organic solvent is added to the raw material, and the temperature is 0 to 100 ° C, more preferably 50 to 100 ° C.
Extract once or repeatedly at 100 ° C for 0.5 to 50 hours. Then, the extraction residue is removed by filtration or centrifugation to obtain an extract, and if necessary, concentrated under reduced pressure, and furthermore, water is removed by treatment such as spray drying or freeze drying to eliminate active oxygen. Prepare a powdered extract with excellent performance. As the hydrophilic organic solvent, for example, methanol, ethanol, propanol, butanol, acetone, acetonitrile, acetic acid, formic acid, and the like can be used. Erasability is reduced. Therefore, it is desirable that the above-mentioned hydrophilic organic solvent be hydrated to have a water content of 30% by volume or more in the case of methanol or ethanol, and 50% by volume or more in the case of other solvents.
In addition, hydrochloric acid, phosphoric acid, acetic acid, or the like is used as a solvent for water extraction in an acidic state, that is, less than pH 7, more preferably pH 6
By using the above-mentioned one, an extract having higher active oxygen scavenging ability can be obtained. In removing the solvent from the extract, the extract component according to the present invention is relatively stable to heat, and is not limited to freeze-drying. However, there is a possibility that the desired effect of the present invention is deteriorated due to deterioration due to moisture absorption or oxidation, and it is desirable to reduce the frequency of contact with air as much as possible.
【００１５】本発明の活性酸素消去用組成物の主体をな
す抽出物は、前述のようにアブラナ科植物の植物体から
抽出して得られるものであるが、これを水などに溶解さ
せ、遠心分離、エタノール沈殿分離、溶剤・分別、シリ
カゲル、アルミナ、活性炭、活性白土等の吸着剤による
分画、イオン交換分離等の精製処理を施すことにより、
抽出物の活性酸素消去能をさらに高めることが可能であ
る。とりわけ、前記方法によって得られる抽出物を水溶
液等の溶液となし、これをイオン交換処理に供して精製
するのがよい。イオン交換処理としては、望ましくは陰
イオン交換能を有する樹脂を用いて吸脱着処理させ、活
性酸素消去能の高い成分を濃縮することができる。The extract which is the main component of the active oxygen scavenging composition of the present invention is obtained by extracting from a plant of the Brassicaceae plant as described above. Separation, ethanol precipitation separation, solvent / fractionation, silica gel, alumina, activated carbon, fractionation with adsorbents such as activated clay, purification treatment such as ion exchange separation,
It is possible to further enhance the ability of the extract to scavenge active oxygen. In particular, it is preferable that the extract obtained by the above method is converted into a solution such as an aqueous solution, and the solution is subjected to an ion exchange treatment for purification. As the ion exchange treatment, a resin having an anion exchange ability is desirably adsorbed and desorbed, and a component having a high ability to scavenge active oxygen can be concentrated.
【００１６】本発明の活性酸素消去用組成物は、前述の
ようにして得られる抽出物を含有せしめて調製する。す
なわち、前記抽出物そのものを本発明の目的物とするこ
とができ、あるいは本発明の所望の作用効果を阻害しな
い公知の素材成分、賦形剤、増量剤、着香料等の各種添
加剤とともに混合もしくは溶解させ、液体状、ペースト
状、粉末状、顆粒状又は固形状の活性酸素消去用組成物
となすこともできる。この場合、本発明に係る抽出物の
配合割合は任意であり、抽出物の活性酸素消去活性、目
的とする組成物の形態、併用する素材や成分の種類等に
より一律に規定しがたいが、利用面での利便性の点から
概ね０．１〜９９重量％、より好ましくは３０〜９０重
量％である。The active oxygen scavenging composition of the present invention is prepared by incorporating the extract obtained as described above. That is, the extract itself can be used as the object of the present invention, or mixed with various additives such as known ingredients, excipients, bulking agents, and flavors that do not inhibit the desired effects of the present invention. Alternatively, the composition may be dissolved to form a liquid, paste, powder, granule, or solid active oxygen eliminating composition. In this case, the blending ratio of the extract according to the present invention is arbitrary, and it is difficult to uniformly define the active oxygen scavenging activity of the extract, the form of the target composition, the type of materials and components used together, etc. From the viewpoint of convenience in use, it is generally 0.1 to 99% by weight, more preferably 30 to 90% by weight.
【００１７】前記の併用素材あるいは成分として望まし
いものは、活性酸素消去能を有する公知の素材である。
この例として、アスコルビン酸（ビタミンＣ）、トコフ
ェロール（ビタミンＥ）、カテキン類（エピガロカテキ
ン、エピガロカテキンガレート、エピカテキン、エピカ
テキンガレート等）、アントシニン類（デルフィニジ
ン、シアニジン、ペチュニジン、ペオニジン、マルビジ
ン、これらの配糖体等）、フラボノイド類（クエルセチ
ン、ルチン、ケンフェロール、ルテオリン、イソフラボ
ン、これらの配糖体等）、これ以外のポリフェノール類
（サポニン、エラグ酸、タンニン等）、緑茶エキス、ほ
うじ茶エキス、ルイボスティーエキス等からなる群から
選ばれる少なくとも１種のものが望ましい。Desirable as the above-mentioned combined material or component is a known material having an active oxygen scavenging ability.
Examples of this include ascorbic acid (vitamin C), tocopherol (vitamin E), catechins (epigallocatechin, epigallocatechin gallate, epicatechin, epicatechin gallate, etc.), anthosines (delphinidin, cyanidin, petunidin, peonidin, malvidin) Flavonoids (quercetin, rutin, kaempferol, luteolin, isoflavone, these glycosides, etc.), other polyphenols (saponin, ellagic acid, tannin, etc.), green tea extract, roasted green tea At least one selected from the group consisting of extract, rooibos extract and the like is desirable.
【００１８】本発明に係る抽出物やこれを含有してなる
活性酸素消去用組成物の活性酸素消去能を評価するには
次に述べる方法を用いるのが簡便である。すなわち、活
性酸素種と活性酸素ラジカル消去物質と活性酸素ラジカ
ル受容種との三種類の物質が共存する系において、活性
酸素種と活性酸素ラジカル消去物質とが化学反応して活
性酸素が安定化する際に生じる微弱発光現象をフォトン
強度として検出する方法（前記の文献参照）や、スーパ
ーオキサイドやヒドロキシラジカル等の活性酸素とＤＭ
ＰＯ（５，５−ジメチル−１−ピロリン−Ｎ−オキサイ
ド）とがオキシダーゼ共存下でＤＭＰＯ−Ｏ２付加体を
形成することを利用したＥＳＲスピントラッピング法等
がある。To evaluate the active oxygen scavenging ability of the extract according to the present invention and the active oxygen scavenging composition containing the same, it is convenient to use the following method. That is, in a system in which three kinds of substances, active oxygen species, active oxygen radical scavenger, and active oxygen radical acceptor coexist, active oxygen species and active oxygen radical scavenger chemically react to stabilize active oxygen. Detecting the weak luminescence phenomenon that occurs at the time as photon intensity (see the above-mentioned document), and using active oxygen such as superoxide or hydroxy radical and DM
There is an ESR spin trapping method utilizing the formation of a DMPO-O 2 adduct with PO (5,5-dimethyl-1-pyrroline-N-oxide) in the presence of oxidase.
【００１９】本発明では、前述のように、アブラナ科植
物の植物体の抽出物を含む活性酸素消去用組成物が提供
されるが、さらにこれを配合してなる組成物も提供され
る。この組成物の態様としては食用組成物、医薬用組成
物、化粧用組成物、その他の工業用組成物等を例示でき
る。これらのうち、食用組成物が好適である。以下に前
記各組成物の例を示すが、本発明はこれらに限定される
ものではない。In the present invention, as described above, a composition for eliminating active oxygen containing an extract of a plant of the Brassicaceae plant is provided, and a composition further comprising the composition is also provided. Examples of the composition include an edible composition, a pharmaceutical composition, a cosmetic composition, and other industrial compositions. Of these, edible compositions are preferred. Examples of the respective compositions are shown below, but the present invention is not limited to these.
【００２０】食用組成物としては、本発明の活性酸素消
去用組成物をそのまま液状、ゲル状あるいは固形状の食
品、例えば清涼飲料、ジュース、茶、ドレッシング、ソ
ース、味噌、醤油、スープ、ゼリー、プリン、ヨーグル
ト、チョコレート、ふりかけ、ガム、キャンディー、ケ
ーキミックス、スナック菓子、粉末状または液体状の乳
製品、パン、クッキー等に添加したり、適宜に澱粉、デ
キストリン、乳糖等の賦形剤や色素、香料等とともに粉
末、顆粒、錠剤、内服液等に加工したり、ゼラチン等の
被覆材を用いてカプセルに成形加工して健康食品、栄養
補助食品や医薬部外品として利用できる。As the edible composition, the active oxygen scavenging composition of the present invention may be used as it is in a liquid, gel or solid food, such as soft drink, juice, tea, dressing, sauce, miso, soy sauce, soup, jelly, etc. Pudding, yogurt, chocolate, sprinkle, gum, candy, cake mix, snacks, powdered or liquid dairy products, bread, cookies, etc., and excipients or pigments such as starch, dextrin, lactose, It can be processed into powders, granules, tablets, oral liquids and the like together with flavors and the like, or formed into capsules using a coating material such as gelatin to be used as health foods, dietary supplements and quasi-drugs.
【００２１】この食用組成物において、本発明の活性酸
素消去用組成物の配合量は、当該食用組成物の種類や状
態等により一律に規定しがたいが、概ね０．１〜５０重
量％、より好ましくは１〜３０重量％である。配合量が
０．１重量％未満では経口摂取による所望の効果が小さ
く、５０重量％を超えると食用組成物の種類によっては
風味を損なったり、当該食用組成物を調製できなくなる
場合がある。なお、本発明の活性酸素消去用組成物それ
自体は、これをそのまま食用組成物として食用に供して
もさしつかえない。In this edible composition, the amount of the active oxygen-scavenging composition of the present invention cannot be uniformly defined depending on the type and state of the edible composition, but is generally about 0.1 to 50% by weight. More preferably, it is 1 to 30% by weight. If the amount is less than 0.1% by weight, the desired effect by oral ingestion is small, and if it exceeds 50% by weight, the flavor may be impaired or the edible composition may not be prepared depending on the type of the edible composition. The active oxygen scavenging composition of the present invention itself may be edible as it is as an edible composition.
【００２２】前記食用組成物以外には、本発明の活性酸
素消去用組成物を粉末状または液体状でクリーム、乳
液、口紅、ファンデーション、日焼け止め液等のサンス
クリーン製品、シャンプー、リンス等に配合して化粧用
組成物となすことができ、また、各種パッキング製品、
シール剤、接着剤、塗装剤等に配合して工業用組成物と
しても利用され得る。In addition to the edible composition, the active oxygen scavenging composition of the present invention is formulated in powder or liquid form into sunscreen products such as creams, emulsions, lipsticks, foundations, sunscreens, shampoos, and rinses. To make a cosmetic composition, and also various packing products,
It can be used as an industrial composition by blending it into a sealant, an adhesive, a coating agent and the like.
【００２３】[0023]
【実施例】実施例１ 生のケールをチップ状に切断し、これに対して５重量倍
の水を加え、９５℃で１時間抽出処理した後、残渣を濾
別して抽出液を得た。ついで、これを凍結乾燥処理する
ことにより黄褐色〜褐色の粉末状の抽出物を調製した。
この抽出物を本発明の活性酸素消去用組成物（試料１）
とした。Example 1 Raw kale was cut into chips, 5 weight times of water was added to the cut, extracted at 95 ° C. for 1 hour, and the residue was filtered to obtain an extract. Then, this was freeze-dried to prepare a yellow-brown to brown powdery extract.
This extract was used as the active oxygen scavenging composition of the present invention (sample 1).
And
【００２４】実施例２ 生のキャベツを実施例１と同様に処理して本発明の活性
酸素消去用組成物（試料２）を調製した。Example 2 A raw cabbage was treated in the same manner as in Example 1 to prepare a composition for scavenging active oxygen of the present invention (Sample 2).
【００２５】実施例３ 生のブロッコリー（全体）を実施例１と同様に処理して
本発明の活性酸素消去用組成物（試料３）を調製した。Example 3 Raw broccoli (whole) was treated in the same manner as in Example 1 to prepare a composition (Sample 3) for erasing active oxygen of the present invention.
【００２６】実施例４ 生の大根の葉部を実施例１と同様に処理して本発明の活
性酸素消去用組成物（試料４）を調製した。Example 4 Raw radish leaves were treated in the same manner as in Example 1 to prepare a composition for scavenging active oxygen of the present invention (Sample 4).
【００２７】実施例５ 生状態のハボタンの白色又は紫色のものを実施例１と同
様に処理して本発明の活性酸素消去用組成物（試料５−
１又は試料５−２）を調製した。Example 5 A white or purple raw habotan was treated in the same manner as in Example 1 to obtain a composition for erasing active oxygen (Sample 5) of the present invention.
1 or sample 5-2) was prepared.
【００２８】実施例６ 生のケールを乾燥させてチップ状に切断したもの（以
下、ケールの乾燥チップという）を原料とし、これに対
して１０重量倍のアルコール濃度：５０容量％の含水エ
タノールを加え、７０℃で１時間抽出処理した後、残渣
を濾別して抽出液を得た。ついで、これを減圧下に乾固
して緑褐食〜褐色の粉末状の抽出物を調製した。この抽
出物を本発明の活性酸素消去用組成物（試料６）とし
た。Example 6 Raw kale was dried and cut into chips (hereinafter referred to as dried kale chips) as a raw material, and 10 parts by weight of an alcohol concentration: 50% by volume of aqueous ethanol was used. In addition, after an extraction treatment at 70 ° C. for 1 hour, the residue was separated by filtration to obtain an extract. Then, this was dried under reduced pressure to prepare a green-brown food-brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 6) of the present invention.
【００２９】実施例７ ケールの乾燥チップに対して１２重量倍のアルコール濃
度：７０容量％の含水メタノールを加え、６５℃で１時
間環流させ抽出処理した後、残渣を濾別して抽出液を得
た。ついで、これを減圧下に乾固して緑褐色〜褐色の粉
末状の抽出物を調製した。この抽出物を本発明の活性酸
素消去用組成物（試料７）とした。Example 7 A 12-fold by weight aqueous methanol solution containing 70% by volume of alcohol was added to dried kale chips, and the mixture was refluxed at 65 ° C. for 1 hour to carry out extraction treatment. The residue was separated by filtration to obtain an extract. . Then, this was dried under reduced pressure to prepare a green-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition of the present invention (Sample 7).
【００３０】実施例８ ケールの乾燥チップに対して１２重量倍の水を加え、５
０℃で１時間抽出処理した後、残渣を濾別して抽出液を
得た。ついで、これを凍結乾燥処理することにより黄褐
色〜褐色の粉末状の抽出物を調製した。この抽出物を本
発明の活性酸素消去用組成物（試料８）とした。Example 8 12 parts by weight of water was added to a dried chip of kale,
After extraction at 0 ° C. for 1 hour, the residue was separated by filtration to obtain an extract. Then, this was freeze-dried to prepare a yellow-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 8) of the present invention.
【００３１】実施例９ ケールの乾燥チップに対して１４重量倍の水を加え、７
０℃で１時間抽出処理した後、残渣を濾別して抽出液を
得た。ついで、これを凍結乾燥処理して黄褐色〜褐色の
粉末状の抽出物を調製した。この抽出物を本発明の活性
酸素消去用組成物（試料９）とした。Example 9 14 weight times of water was added to the dried chip of kale,
After extraction at 0 ° C. for 1 hour, the residue was separated by filtration to obtain an extract. Then, this was freeze-dried to prepare a yellow-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 9) of the present invention.
【００３２】実施例１０ ケールの乾燥チップに対して１３重量倍の水を加え、９
５℃で３時間抽出処理した後、残渣を濾別して抽出液を
得た。ついで、これを凍結乾燥処理して黄褐色〜褐色の
粉末状の抽出物を調製した。この抽出物を本発明の活性
酸素消去用組成物（試料１０）とした。Example 10 Water was added at 13 times the weight of a dried chip of kale to give 9
After extraction at 5 ° C. for 3 hours, the residue was separated by filtration to obtain an extract. Then, this was freeze-dried to prepare a yellow-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 10) of the present invention.
【００３３】実施例１１ 耐圧性容器にケールの乾燥チップを仕込み、該チップに
対して１２重量倍の水を加え、約２ｋｇ／ｃｍ２の加圧
下１２５℃で１時間抽出処理した後、残渣を濾別して抽
出液を得た。ついで、これを凍結乾燥処理して黄褐色〜
褐色の粉末状の抽出物を調製した。この抽出物を本発明
の活性酸素消去用組成物（試料１１）とした。Example 11 A kale dried chip was charged into a pressure-resistant container, and water was added at 12 times the weight of the chip, followed by extraction at 125 ° C. under a pressure of about 2 kg / cm 2 for 1 hour. An extract was obtained by filtration. Then, this is freeze-dried, and
A brown powdery extract was prepared. This extract was used as the active oxygen eliminating composition (sample 11) of the present invention.
【００３４】実施例１２ ケールの乾燥チップに対して１２重量倍の水を加え、室
温で１５時間抽出処理した後、残渣を濾別して抽出液を
得た。この残渣に７重量倍の水を加え、同様に室温で１
５時間抽出ついで残渣濾別を行い抽出液を得た。両抽出
液をあわせ凍結乾燥することにより黄褐色〜褐色の粉末
状の抽出物を調製した。この抽出物を本発明の活性酸素
消去用組成物（試料１２）とした。Example 12 Water was added at 12 times the weight of a dried chip of kale, and the mixture was extracted at room temperature for 15 hours. The residue was separated by filtration to obtain an extract. To this residue was added 7 weight times of water, and
After extraction for 5 hours, the residue was separated by filtration to obtain an extract. Both extracts were combined and freeze-dried to prepare a yellow-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 12) of the present invention.
【００３５】実施例１３ ケールの乾燥チップに対して１２重量倍の１０重量％酢
酸水溶液を加え、７０℃で１時間抽出処理した後、残渣
を濾別して抽出液を得た。ついで、これを減圧下に乾固
して緑褐色〜褐色の粉末状の抽出物を調製した。この抽
出物を本発明の活性酸素消去用組成物（試料１３）とし
た。Example 13 A 10% by weight aqueous solution of acetic acid was added to a dried chip of kale at a weight ratio of 12% by weight. After extraction at 70 ° C. for 1 hour, the residue was separated by filtration to obtain an extract. Then, this was dried under reduced pressure to prepare a green-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 13) of the present invention.
【００３６】実施例１４ ケールの乾燥チップに対して１２重量倍の０．０５重量
％水酸化ナトリウム水溶液を加え、７０℃で１時間抽出
処理した後、残渣を濾別して抽出液を得た。ついで、こ
れを減圧下に乾固して緑褐色〜褐色の粉末状の抽出物を
調製した。この抽出物を本発明の活性酸素消去用組成物
（試料１４）とした。Example 14 A 0.05% by weight aqueous solution of sodium hydroxide was added to a dried chip of kale at a weight ratio of 12% by weight. After extraction at 70 ° C. for 1 hour, the residue was separated by filtration to obtain an extract. Then, this was dried under reduced pressure to prepare a green-brown to brown powdery extract. This extract was used as the active oxygen eliminating composition (sample 14) of the present invention.
【００３７】実施例１５ 試料１０をその濃度が４０重量％となるように水に溶解
させ、室温にて約３時間静置後、生じた沈殿物を遠心分
離して除き、上澄液を採取した。ついで、これを凍結乾
燥することにより粉末状の精製抽出物を調製した。この
精製物を本発明の活性酸素消去用組成物（試料１５）と
した。Example 15 Sample 10 was dissolved in water to a concentration of 40% by weight, allowed to stand at room temperature for about 3 hours, the resulting precipitate was removed by centrifugation, and the supernatant was collected. did. Then, this was freeze-dried to prepare a powdery purified extract. This purified product was used as the active oxygen eliminating composition of the present invention (sample 15).
【００３８】実施例１６ 実施例１５で得た粉末状の精製抽出物をその濃度が２０
重量％となるように水に溶解させ、これにアルコール濃
度が５０重量％となるようにエタノールを加えた後、生
じた沈殿物を遠心分離して除去し、上澄液を採取した。
ついで、これを凍結乾燥することにより、粉末状の精製
抽出物を調製した。この精製物を本発明の活性酸素消去
用組成物（試料１６）とした。Example 16 The powdery purified extract obtained in Example 15 was used at a concentration of 20.
The resulting precipitate was dissolved in water to a concentration of 50% by weight, and ethanol was added thereto to adjust the alcohol concentration to 50% by weight. The resulting precipitate was removed by centrifugation, and the supernatant was collected.
Then, this was freeze-dried to prepare a powdery purified extract. This purified product was used as the active oxygen eliminating composition of the present invention (sample 16).
【００３９】実施例１７ 試料１０をその濃度が１０重量％となるように水に溶解
させ、予め常法により活性化させた陰イオン交換樹脂
（バイオラッド社、ＡＧ１−Ｘ８）を充填したオープン
カラム管に注入し、水を移動相として１ｍｌ／分の割合
で流した。この操作を３０分間続け、移動相を６重量％
酢酸水溶液にきりかえ、吸着成分を溶離させた。この酢
酸分画分を凍結乾燥処理することにより粉末状の精製抽
出物を調製した。この精製物を本発明の活性酸素消去用
組成物（試料１７）とした。Example 17 An open column filled with an anion exchange resin (Bio-Rad, AG1-X8), which was prepared by dissolving the sample 10 in water so that the concentration thereof became 10% by weight, and previously activated by a conventional method. The tube was injected and water was flowed at a rate of 1 ml / min as a mobile phase. This operation was continued for 30 minutes, and the mobile phase was reduced to 6% by weight.
The solution was replaced with an aqueous acetic acid solution to elute the adsorbed components. This acetic acid fraction was freeze-dried to prepare a powdery purified extract. This purified product was used as the active oxygen eliminating composition (Sample 17) of the present invention.
【００４０】実施例１８ 市販の緑茶葉を８０℃の熱水で１時間抽出処理し、残渣
を分離して緑茶抽出液を得た。ついで、これを噴霧乾燥
処理して緑黄褐色の粉末状の緑茶エキスをつくった。こ
の緑茶エキスと、実施例１０に記載の方法により調製し
た粉末状の抽出物とを２０：８０（重量比）の割合で十
分に混合して本発明の活性酸素用組成物（試料１８）と
した。Example 18 A commercially available green tea leaf was subjected to extraction treatment with hot water at 80 ° C. for 1 hour, and the residue was separated to obtain a green tea extract. Then, this was spray-dried to prepare a green-yellow-brown powdered green tea extract. This green tea extract and the powdery extract prepared by the method described in Example 10 were sufficiently mixed at a ratio of 20:80 (weight ratio) to obtain a composition for active oxygen of the present invention (sample 18). did.
【００４１】実施例１９ 本発明の粉末状活性酸素消去用組成物（試料９）１ｋｇ
に１５重量％還元麦芽糖水飴（マルチトール）水溶液を
噴霧し、高速撹拌造粒機（フカエパウレック社製、ハイ
スピードミキサー）に供して造粒化した後、乾燥及び篩
過（１０〜８０タイラー・メッシュ）して顆粒状の食用
組成物を試作した。これは野菜類特有の青臭さがなく、
風味、食感ともに良好であり、体内の活性酸素を消去
し、活性酸素の作用によって誘発される各種疾患の予防
用食品として好適に利用され得るものである。Example 19 1 kg of the powdery active oxygen scavenging composition of the present invention (sample 9)
Is sprayed with a 15% by weight aqueous solution of reduced maltose syrup (maltitol), granulated by a high-speed stirring granulator (Fukae Powrex, high speed mixer), dried and sieved (10-80 Tyler mesh). ) To produce a granulated edible composition. This has no peculiar smell of vegetables,
It has good flavor and texture, eliminates active oxygen in the body, and can be suitably used as a food for preventing various diseases induced by the action of active oxygen.
【００４２】実施例２０ 本発明の粉末状活性酸素消去用組成物（試料１０）２ｇ
を市販の番茶０．２リットルに添加し、十分に混合して
均質な飲料物を試作した。このものは通常の番茶と比較
して風味、色あい、食感等の食品適性に遜色なく、活性
酸素消去およびこれに関係する種々の疾病の予防のため
の飲料として利用され得る。Example 20 2 g of the powdery active oxygen scavenging composition of the present invention (sample 10)
Was added to commercially available Bancha (0.2 liter) and mixed well to produce a homogeneous beverage. It can be used as a beverage for elimination of active oxygen and prevention of various diseases related thereto, as good as food taste such as flavor, color and texture as compared with ordinary bancha.
【００４３】比較例１ 生のトマトの実を軽くつぶし、これを原料とし、実施例
１と同様に処理して粉末状の抽出組成物（比較試料１）
を得た。Comparative Example 1 A raw tomato fruit was lightly crushed and used as a raw material, and treated in the same manner as in Example 1 to obtain a powdery extract composition (Comparative Sample 1).
I got
【００４４】比較例２ 生のシソの葉をチップ状に切断し、これを原料とし、実
施例１と同様に処理して粉末状の抽出組成物（比較試料
２）を得た。Comparative Example 2 Raw perilla leaves were cut into chips, and this was used as a raw material and treated in the same manner as in Example 1 to obtain a powdery extract composition (Comparative Sample 2).
【００４５】比較例３ ケールを原料とした市販の青汁（田辺食品（株）製、乾
燥粉末品）を本発明品の比較物として用いた。Comparative Example 3 A commercially available green juice (dried powder product, manufactured by Tanabe Foods Co., Ltd.) using kale as a raw material was used as a comparative product of the product of the present invention.
【００４６】試験例１ 試作した各活性酸素消去用組成物（試料１〜１７）及び
各抽出組成物（比較試料１〜３）の活性酸素消去能を次
に述べる方法により評価した。すなわち、水を溶媒と
し、活性酸素種として２重量％過酸化水素水１ｍｌ、ラ
ジカル受容種として飽和炭酸水素カリウム１ｍｌ、及び
活性酸素ラジカル消去物として１重量％の本発明の活性
酸素消去用組成物又は比較対象物１ｍｌをマイクロプレ
ートタイプのウエル型ホール中で混合して測定用サンプ
ルとした。該サンプルは調製後ただちに、化学反応にと
もない生じる微弱な化学発光によるフォトン（光子）を
高感度で検出することができるフォトンカウンティング
カメラ（ＶＩＭカメラ）、イメージプロセッサー、デー
タ解析装置及びモニターディスプレー装置を搭載した発
光測定装置（浜松ホトニクス（株）製、ＡＲＧＵＳ−５
０／ＶＩＭシステム）に供し、発光強度を１０分間蓄積
測定した。この結果を表１〜３に示す。同表において、
発光強度は単位発光面積（１μｍ２）あたりのフォトン
比例数である。なお、測定時の温度は室温とした。Test Example 1 The active oxygen scavenging ability of each of the test active oxygen scavenging compositions (Samples 1 to 17) and each of the extracted compositions (Comparative Samples 1 to 3) was evaluated by the following method. That is, using water as a solvent, 1 ml of a 2% by weight aqueous hydrogen peroxide solution as an active oxygen species, 1 ml of saturated potassium hydrogen carbonate as a radical accepting species, and 1% by weight of an active oxygen radical scavenger of the present invention Alternatively, 1 ml of the comparative object was mixed in a well of a microplate type to obtain a measurement sample. Immediately after preparation, the sample is equipped with a photon counting camera (VIM camera), image processor, data analyzer, and monitor display device that can detect photons (photons) due to weak chemiluminescence generated by a chemical reaction with high sensitivity. Luminescence measuring device (ARGUS-5, manufactured by Hamamatsu Photonics KK)
0 / VIM system), and the emission intensity was measured for 10 minutes. The results are shown in Tables 1 to 3. In the table,
The emission intensity is a proportional number of photons per unit emission area (1 μm 2 ). The temperature during the measurement was room temperature.
【００４７】試料１〜５−２及び比較試料１、２の活性
酸素消去能を表１に、試料６〜１４及び比較試料３の活
性酸素消去能を表２に、また、試料１５〜１８の活性酸
素消去能を表３にそれぞれ示す。Table 1 shows the active oxygen scavenging ability of Samples 1 to 5-2 and Comparative Samples 1 and 2, Table 2 shows the active oxygen scavenging ability of Samples 6 to 14 and Comparative Sample 3, and Table 15 to 18 of Samples 15 to 18. Table 3 shows the active oxygen scavenging ability.
【００４８】[0048]
【表１】 [Table 1]
【００４９】なお、前記の測定系において測定されるフ
ォトン比例数は、活性酸素が活性酸素消去物質と化学反
応して安定化する際に生じる発光の強さを示すものであ
るから、該発光強度は活性酸素を消去する活性の強さに
比例するものであることが知られている（前出の文献、
安本教傅ら編「２１世紀の栄養・食糧科学を展望す
る」、日本食品出版（株）、５６〜６４頁、１９９９年
など）。したがって、表１のデータから、本発明に係る
アブラナ科植物の植物体の抽出物を含んでなる組成物
は、発光強度が大きく、活性酸素を消去する能力に優れ
ていることが明らかになった。とりわけ、アブラナ科植
物としてケール、ハボタン、ブロッコリー等の抽出物で
は顕著な効果を奏することが認められた。The photon proportional number measured in the above-described measurement system indicates the intensity of light emission generated when active oxygen is chemically reacted with an active oxygen scavenger and stabilized, so that the light emission intensity Is known to be proportional to the activity of scavenging active oxygen.
"Prospects for Nutrition and Food Science in the 21st Century," edited by K. Fumoto et al., Japan Food Publishing Co., Ltd., pp. 56-64, 1999. Therefore, from the data in Table 1, it was revealed that the composition comprising the extract of the plant of the Brassicaceae plant according to the present invention has a high emission intensity and an excellent ability to scavenge active oxygen. . In particular, it has been found that extracts such as kale, haboton, broccoli and the like as cruciferous plants have remarkable effects.
【００５０】[0050]
【表２】 [Table 2]
【００５１】表２のデータから、ケールを乾燥させて切
断したものを原料とし、望ましくは酸性の水又は含水親
水性有機溶媒を用いて、５０〜１００℃で抽出して得ら
れる抽出物を含んでなる組成物は、活性酸素を消去する
能力に優れていることが明らかになった。また、前記抽
出物と緑茶エキス等の活性酸素消去能ある素材成分とを
併用することにより所望の効果はより顕著に発現され
る。From the data shown in Table 2, the extract obtained by extracting kale by drying and cutting it at 50 to 100 ° C. using acidic water or a water-containing hydrophilic organic solvent is preferably used as a raw material. It has been found that the composition consisting of has excellent ability to scavenge active oxygen. In addition, the desired effect is more remarkably exhibited by using the extract in combination with a material component capable of eliminating active oxygen such as green tea extract.
【００５２】[0052]
【表３】 [Table 3]
【００５３】表３のデータから、本発明に係る抽出物に
対し、沈殿物除去、陰イオン交換カラム処理等の精製処
理を行うと、これにより得られる活性酸素消去用組成物
は、発光強度がより一層高まることが明らかになった。From the data shown in Table 3, when the extract according to the present invention is subjected to purification treatment such as removal of precipitate and treatment with an anion exchange column, the composition for scavenging active oxygen obtained therefrom has a low luminescence intensity. It became clear that it would be even higher.
【００５４】[0054]
【発明の効果】本発明によれば、アブラナ科植物の植物
体を水及び／又は親水性有機溶媒を用いて抽出して得ら
れる抽出物を含有してなる活性酸素消去用組成物が提供
される。ここで、アブラナ科植物としては特にケールを
原料とし、また酸性の前記抽出溶媒を用いるとき、より
顕著な活性酸素消去活性を有する活性酸素消去用組成物
が得られる。さらに、前記抽出物と活性酸素消去活性の
ある公知の素材成分を併用すると、より一層顕著な活性
酸素消去能を示す組成物となる。アブラナ科植物の植物
体を原料として、望ましくは酸性の水及び／又は親水性
有機溶媒を用いて５０〜１００℃で抽出し、残渣を除去
して得られる抽出液を好適にはアルコール分別して沈殿
物を除去し、あるいはイオン交換処理に供した後に凍結
乾燥もしくは噴霧乾燥等の粉末化処理をすることを特徴
とする、前記効果を奏する活性酸素消去用組成物の製造
方法が提供される。さらに、本発明によれば、前記活性
酸素消去用組成物を配合してなる食用組成物を提供でき
る。この食用組成物は、経口摂取することにより体内の
活性酸素消去や活性酸素に起因する各種疾患の予防用食
品として利用され得るものである。Industrial Applicability According to the present invention, there is provided a composition for eliminating active oxygen, comprising an extract obtained by extracting a cruciferous plant using water and / or a hydrophilic organic solvent. You. Here, particularly as a cruciferous plant, when kale is used as a raw material and the acidic extraction solvent is used, a composition for eliminating active oxygen having a more remarkable activity for eliminating active oxygen can be obtained. Furthermore, when the extract is used in combination with a known material component having active oxygen scavenging activity, a composition exhibiting an even more remarkable active oxygen scavenging ability is obtained. Extraction is performed at 50 to 100 ° C., preferably using acidic water and / or a hydrophilic organic solvent, using a plant of the cruciferous plant as a raw material, and an extract obtained by removing the residue is preferably subjected to alcohol fractionation to precipitate. A method for producing a composition for elimination of active oxygen, which has the above-mentioned effect, characterized in that a powdery treatment such as freeze-drying or spray-drying is carried out after removing substances or subjecting to ion exchange treatment. Further, according to the present invention, an edible composition comprising the active oxygen eliminating composition can be provided. This edible composition can be used as a food for eliminating active oxygen in the body or preventing various diseases caused by active oxygen when taken orally.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ａ６１Ｋ 35/78 Ａ６１Ｋ 35/78 Ｃ ４Ｈ０２５ 45/00 45/00 Ａ６１Ｐ 39/06 Ａ６１Ｐ 39/06 Ｂ０１Ｄ 11/04 Ｂ０１Ｄ 11/04 Ｃ Ｃ０９Ｋ 15/34 Ｃ０９Ｋ 15/34 Ｆターム(参考） 4B021 MC04 MK02 MK05 MK16 MK26 MP01 MQ04 4C084 AA16 MA02 MA43 MA52 NA05 ZB211 ZB212 ZC371 ZC372 4C086 AA01 AA02 BA08 BA09 BA18 MA02 MA04 MA43 MA52 NA05 ZB21 ZC37 4C088 AB15 AB45 AC01 AC05 CA04 CA05 CA14 MA02 MA03 MA07 MA43 MA52 NA05 NA14 ZB21 ZC37 4D056 AB18 AB19 AC06 AC09 AC21 AC22 CA33 DA01 DA05 DA06 4H025 AA18 AA82 AA83 AC05 BA01──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 7 Identification symbol FI Theme coat ゛ (Reference) A61K 35/78 A61K 35/78 C 4H025 45/00 45/00 A61P 39/06 A61P 39/06 B01D 11 / 04 B01D 11/04 C C09K 15/34 C09K 15/34 F term (reference) 4B021 MC04 MK02 MK05 MK16 MK26 MP01 MQ04 4C084 AA16 MA02 MA43 MA52 NA05 ZB211 ZB212 ZC371 ZC372 4C086 AA01 AA02 MA08 BA09 MA18 MA02 NA08 4C088 AB15 AB45 AC01 AC05 CA04 CA05 CA14 MA02 MA03 MA07 MA43 MA52 NA05 NA14 ZB21 ZC37 4D056 AB18 AB19 AC06 AC09 AC21 AC22 CA33 DA01 DA05 DA06 4H025 AA18 AA82 AA83 AC05 BA01
Claims (10)
してなる活性酸素消去用組成物。1. A composition for eliminating active oxygen, comprising an extract of a plant of the Brassicaceae plant.
ロッコリー及びカリフラワーからなる群から選ばれる１
種又は２種以上である請求項１に記載の活性酸素消去用
組成物。2. The cruciferous plant is selected from the group consisting of kale, havoon, broccoli and cauliflower.
The active oxygen eliminating composition according to claim 1, wherein the composition is at least one species.
用いて抽出処理して得られる粉末状エキスである請求項
１に記載の活性酸素消去用組成物。3. The active oxygen eliminating composition according to claim 1, wherein the extract is a powdery extract obtained by performing an extraction treatment using water and / or a hydrophilic organic solvent.
のである請求項３に記載の活性酸素消去用組成物。4. The active oxygen scavenging composition according to claim 3, wherein the water and / or the hydrophilic organic solvent is acidic.
たものである請求項１又は３に記載の活性酸素消去用組
成物。5. The active oxygen eliminating composition according to claim 1, wherein the extract is purified through an ion exchange treatment.
性酸素消去能を有する公知の素材とを少なくとも含有し
てなる活性酸素消去用組成物。6. An active oxygen-scavenging composition comprising at least an extract of a plant of the Brassicaceae plant and a known material having an active oxygen-scavenging ability.
アスコルビン酸、トコフェロール、カテキン類、アント
シアニン類、フラボノイド類、これ以外のポリフェノー
ル類、緑茶エキス、ほうじ茶エキス及びルイボスティー
エキスからなる群から選ばれる少なくとも１種である請
求項６に記載の活性酸素消去用組成物。7. A known material having an active oxygen scavenging ability,
The active oxygen elimination according to claim 6, which is at least one selected from the group consisting of ascorbic acid, tocopherol, catechins, anthocyanins, flavonoids, other polyphenols, green tea extract, roasted tea extract and rooibos tea extract. Composition.
親水性有機溶媒を用いて０〜１００℃で抽出することを
特徴とする活性酸素消去用組成物の製造法。8. A process for producing a composition for eliminating active oxygen, comprising extracting a plant of a cruciferous plant at 0 to 100 ° C. using water and / or a hydrophilic organic solvent.
／又は親水性有機溶媒を用いて抽出し、該抽出物をイオ
ン交換処理に供することを特徴とする請求項８に記載の
活性酸素消去用組成物の製造法。9. The activity according to claim 8, wherein a cut product of the dried kale is extracted using acidic water and / or a hydrophilic organic solvent, and the extract is subjected to an ion exchange treatment. A method for producing an oxygen scavenging composition.
活性酸素消去用組成物を配合してなる食用組成物。10. An edible composition comprising the active oxygen scavenging composition according to any one of claims 1 to 7.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000159136A JP2001299305A (en) | 2000-04-19 | 2000-04-19 | Composition for scavenging active oxygen, and method for producing the same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000159136A JP2001299305A (en) | 2000-04-19 | 2000-04-19 | Composition for scavenging active oxygen, and method for producing the same |
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|---|---|
| JP2001299305A true JP2001299305A (en) | 2001-10-30 |
Family
ID=18663502
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|---|---|---|---|
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003081848A (en) * | 2001-09-13 | 2003-03-19 | Maruzen Pharmaceut Co Ltd | Active oxygen-eliminating agent, and cosmetic and food and drink containing the formulated active oxygen- eliminating agent |
| JP2003327539A (en) * | 2002-05-10 | 2003-11-19 | Fancl Corp | Oral dose composition |
| WO2004052385A1 (en) * | 2002-12-06 | 2004-06-24 | Sunstar Inc. | Composition containing green and yellow vegetables and light-colored vegetables |
| WO2004083857A1 (en) * | 2003-03-17 | 2004-09-30 | Shizuoka Prefecture | Method of assaying physiological activity and apparatus |
| EP1512406A1 (en) * | 2003-09-03 | 2005-03-09 | Cognis Iberia, S.L. | Preparations for oral ingestion |
| WO2006055550A2 (en) * | 2004-11-15 | 2006-05-26 | Borba, Llc | Nutritional beverage with skin improvement aids |
| JP2006199610A (en) * | 2005-01-19 | 2006-08-03 | Kao Corp | Wrinkle-improving agent |
| WO2008078451A1 (en) * | 2006-12-22 | 2008-07-03 | Takasago International Corporation | Two-part antioxidant composition and antioxidant product comprising the same |
| EP2226076A1 (en) * | 2009-02-25 | 2010-09-08 | Henning Vollert | Plant Extract for the Prophylaxis and Treatment of Hyperglycemic Diseases |
| US8012510B2 (en) * | 2003-06-13 | 2011-09-06 | Canthao Corporation | Nutraceutical for the prevention and treatment of cancers and diseases affecting the liver |
| JP2017534287A (en) * | 2014-11-07 | 2017-11-24 | 株式会社ファミニティー | Detoxifying composition containing new green extract as active ingredient |
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2000
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003081848A (en) * | 2001-09-13 | 2003-03-19 | Maruzen Pharmaceut Co Ltd | Active oxygen-eliminating agent, and cosmetic and food and drink containing the formulated active oxygen- eliminating agent |
| JP2003327539A (en) * | 2002-05-10 | 2003-11-19 | Fancl Corp | Oral dose composition |
| EP1576960A4 (en) * | 2002-12-06 | 2007-10-17 | Sunstar Inc | Composition containing green and yellow vegetables and light-colored vegetables |
| WO2004052385A1 (en) * | 2002-12-06 | 2004-06-24 | Sunstar Inc. | Composition containing green and yellow vegetables and light-colored vegetables |
| JPWO2004052385A1 (en) * | 2002-12-06 | 2006-04-06 | サンスター株式会社 | Composition containing green-yellow vegetable and light-colored vegetable |
| US7736678B2 (en) | 2002-12-06 | 2010-06-15 | Sunstar Inc. | Method of enhancing blood antioxidant activity ingesting a compound in the form of at least one form selected from amongst juice, powder, granule, tablet and capsule, which contains an effective amount of at least one vegetable selected from the group consisting of broccoli, spinach, parsley, komatsuna (Brassicad rapa L.) and japanese radish leaves, and at least one vegetable selected from amongst lettuce, cabbage and celery |
| WO2004083857A1 (en) * | 2003-03-17 | 2004-09-30 | Shizuoka Prefecture | Method of assaying physiological activity and apparatus |
| US8202545B2 (en) | 2003-06-13 | 2012-06-19 | Canthao Corporation | Nutraceutical for the prevention and treatment of cancers and diseases affecting the liver |
| US8012510B2 (en) * | 2003-06-13 | 2011-09-06 | Canthao Corporation | Nutraceutical for the prevention and treatment of cancers and diseases affecting the liver |
| EP1512406A1 (en) * | 2003-09-03 | 2005-03-09 | Cognis Iberia, S.L. | Preparations for oral ingestion |
| WO2006055550A3 (en) * | 2004-11-15 | 2006-10-26 | Borba Llc | Nutritional beverage with skin improvement aids |
| WO2006055550A2 (en) * | 2004-11-15 | 2006-05-26 | Borba, Llc | Nutritional beverage with skin improvement aids |
| JP2006199610A (en) * | 2005-01-19 | 2006-08-03 | Kao Corp | Wrinkle-improving agent |
| WO2008078451A1 (en) * | 2006-12-22 | 2008-07-03 | Takasago International Corporation | Two-part antioxidant composition and antioxidant product comprising the same |
| JP2008156278A (en) * | 2006-12-22 | 2008-07-10 | Takasago Internatl Corp | Two-pack type antioxidant composition and antioxidant product containing the same |
| EP2226076A1 (en) * | 2009-02-25 | 2010-09-08 | Henning Vollert | Plant Extract for the Prophylaxis and Treatment of Hyperglycemic Diseases |
| JP2017534287A (en) * | 2014-11-07 | 2017-11-24 | 株式会社ファミニティー | Detoxifying composition containing new green extract as active ingredient |
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