JP2003081848A - Active oxygen-eliminating agent, and cosmetic and food and drink containing the formulated active oxygen- eliminating agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a natural active oxygen-eliminating agent having excellent active oxygen-eliminating activities based on a superoxide-eliminating activities, hydrogen peroxide-eliminating activities and free radical-eliminating activities, having high safety and prepared from an easily available raw material. SOLUTION: This natural active oxygen-eliminating agent containing a superoxide-eliminating material, a hydrogen peroxide-eliminating material or a free radical-eliminating material as an active ingredient is extracted from a flower part, a seed, a leaf part, a stem part or an aboveground part including them of a plant belonging to the genus Brassica of Brassicaceae, especially Brassica rope. The cosmetic and the food and drink contain the formulated active oxygen-eliminating agent.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an active oxygen scavenger having an excellent active oxygen scavenging action based on superoxide scavenging action, hydrogen peroxide scavenging action and radical scavenging action, and cosmetics containing the active oxygen scavenger. And food and drink. In the present invention, the food and drink means
It refers to a wide range of oral foods, health foods, foods with health claims, quasi drugs and medicines.

[0002]

2. Description of the Related Art Active oxygen is generated in the process of energy metabolism in living cells, and includes superoxide [that is, superoxide anion (.O ^2- ) generated by one-electron reduction of oxygen molecule], hydrogen peroxide ( H ₂ O ₂ ) and hydroxy radicals (.OH). These active oxygens are essential for the bactericidal mechanism of phagocytes and play an important role in the removal of viruses and cancer cells, but excessive production of active oxygen attacks the biomolecules that make up the membranes and tissues in the body. However, it may induce various diseases. For example, active oxygen is considered to decompose, denature or cross-link living tissues such as collagen and to oxidize oils and fats to produce lipid peroxides that damage cells, and these are caused by active oxygen. Is considered to cause aging such as wrinkling of the skin and reduction of elasticity of the skin, inflammation, and skin pigmentation.

It has been confirmed that glutathione, vitamins C, E, B ₆ , butylhydroxytoluene, butylhydroxyaniline and the like are effective as such substances having an active oxygen scavenging effect, but all of them are cosmetics. There is a problem in the stability and safety when blended in. In addition, various natural products have been reported as substances having an active oxygen scavenging action. For example, baicalein in sardine, plants of the family Rosaceae, fruit juice of piercing pears, extracts of the family Beluga (beech) and / or beetle (beech), Kakishibu (JP-A-64-50877, JP-A-3-8354).
No. 8, JP-A-5-316963, JP-A-200
No. 0-290190, etc.) has been proposed.

However, there is a strong demand from consumers for an active oxygen scavenger which is highly effective and safe and whose raw materials are easily available, and at present it is not possible to provide a sufficiently satisfactory one.

Further, Japanese Unexamined Patent Publication (Kokai) No. 8-325130 describes that an extract of Brassica genus Brassica has a whitening effect and an anti-inflammatory effect.
It has not been disclosed or suggested that it has an active oxygen scavenging action.

[0006]

SUMMARY OF THE INVENTION Under such circumstances, it is an object of the present invention to solve various problems in the prior art and achieve the following objects.

That is, the present invention has a superior active oxygen scavenging action based on the superoxide scavenging action, hydrogen peroxide scavenging action and radical scavenging action, is highly safe and is a natural active oxygen scavenger whose raw material is easily available. And to provide cosmetics and foods and drinks containing the active oxygen scavenger.

[0008]

Means for Solving the Problems As a result of intensive studies to achieve the above object, the inventors of the present invention have found that the Brassica family Brassica plants, particularly rape blossoms (Brass).
The extract of the flower part, seed, leaf part, stem part or the above-ground part containing these has a superoxide scavenging action, a hydrogen peroxide scavenging action and a radical scavenging action, and is useful as an active oxygen scavenger. It was discovered that they existed, and the present invention was accomplished.

[0009] That is, to solve the above problems, the present invention provides the following active oxygen scavenger, cosmetics and food and drink containing the active oxygen scavenger.

[0010] The invention of claim 1 is an active oxygen scavenger characterized by containing a superoxide scavenging substance extracted from a plant of the Brassica family Brassica as an active ingredient.

A second aspect of the present invention is an active oxygen scavenger characterized by containing a hydrogen peroxide scavenging substance extracted from a plant of the Brassicaceae genus Brassica as an active ingredient.

[0012] A third aspect of the present invention is an active oxygen scavenger characterized by containing a radical scavenging substance extracted from a plant of the Brassicaceae genus Brassica as an active ingredient.

The invention of claim 4 relates to rape blossoms (Brassi)
(Caropa) flower part, seed, leaf part, stalk part, or above-ground part containing these is obtained by extracting with water, a hydrophilic organic solvent, or these mixed solvents. It is the active oxygen scavenger described.

A fifth aspect of the present invention is a cosmetic, characterized by comprising the active oxygen scavenger according to any one of the first to fourth aspects.

A sixth aspect of the present invention is a food or drink characterized in that it contains the active oxygen scavenger according to any one of the first to fourth aspects.

[0016] The lipoxygenase activity-reducing action of the extract of Brassicaceae Brassica described in Japanese Patent Laid-Open No. 8-325130 suppresses the action of lipoxygenase reacting with free arachidonic acid to cause inflammation. It also prevents inflammation and suggests that Brassicaceae Brassica plants (especially rapeseed) have excellent active oxygen scavenging action based on superoxide scavenging action, hydrogen peroxide scavenging action and radical scavenging action. Nonetheless, the effects of preventing aging such as wrinkling of the skin and reduction in elasticity of the skin due to these actions are not known at all, and these are new findings by the present inventors.

[0017]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in more detail below. The active oxygen scavenger of the present invention contains, as active ingredients, a superoxide scavenger, a hydrogen peroxide scavenger, and a radical scavenger, which are extracted from plants of the Brassica family Brassica.

Here, the brassica family Brassica (Brass)
ica) plants include, for example, rape (Brass)
ica ropa), white mustard (Brassica hi)
rta), yellow mustard (Brassica junce)
a), black mustard (Brassica nigra) and the like, and one of these may be used alone or two or more thereof may be used in combination. Among these, rapeseed (Brassica r) is highly effective in removing active oxygen.
most preferred is opa).

Here, rapeseed is a plant belonging to the genus Brassica of the Brassicaceae family, also called rapeseed or rapeseed, and is a very familiar and safe plant that can be eaten as it is and used for oils, pigments and the like. .

As a raw material for extracting the active oxygen scavenger of the present invention, plants of the genus Brassica, particularly rapeseed flowers, seeds, leaves, stems, or above-ground parts or roots containing them are used. Although it can be used, the most preferable one is the above-ground part (flower part, seed, leaf part, stem part or a mixed part thereof) of rapeseed.

[0021] It has not yet been confirmed what kind of compound is contained in the rapeseed which is the above-mentioned extraction raw material and exhibits an active oxygen scavenging action, but it is the flower part, seed, leaf part, stem part of rapeseed, or The above-ground part containing them is put into water or a hydrophilic organic solvent or a mixed solvent thereof, and extracted by using an arbitrary device at room temperature to a temperature not higher than the boiling point of the solvent. The rapeseed, which is an extraction raw material to be subjected to the extraction treatment, is preferably dried and crushed or roughly crushed in advance, and may be degreased with a nonpolar solvent such as hexane.

The water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those that have been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone;
1,3-butylene glycol, propylene glycol,
Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin, and mixed solvents of these hydrophilic organic solvents and water can be used.

When a mixed solvent of water and a hydrophilic organic solvent is used, 1 to 90 parts by mass relative to 10 parts by mass of water in the case of lower alcohol, and 10 parts by mass of water in the case of lower aliphatic ketone. It is preferable to add 1 to 40 parts by weight to 10 parts by weight, and in the case of polyhydric alcohol, 1 to 90 parts by weight to 10 parts by weight of water.

Specifically, the dried / ground product of the above-ground portion of rapeseed is put into a treatment tank filled with an extraction solvent, and the mixture is allowed to stand for 30 minutes to 4 hours with occasional stirring, if necessary, to dissolve soluble components. After elution, the solid matter is removed by filtration, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of extraction solvent is usually 5 to 15 of the extraction raw material.
The amount is preferably double (mass ratio), and the extraction condition is usually 50 to 95 ° C. and about 1 to 4 hours when water is used as the extraction solvent. Moreover, when using the mixed solvent of water and ethanol as an extraction solvent, it is usually 40-80.
It is about 30 minutes to 4 hours at ° C.

When the solvent is distilled off from the extract obtained by filtration or centrifugation, a paste-like concentrate is obtained. Further drying gives a solid extract. However, the rapeseed extract of the present invention does not have to be a solid extract, and may be in the state of the above extract or its concentrate. These may be used after being purified by a method such as activated carbon treatment, adsorption resin treatment, ion exchange resin, liquid-liquid countercurrent distribution, etc., provided that the object of the present invention is not hindered.

The rapeseed extract obtained or a product obtained by appropriately purifying the same can be used as it is as the active oxygen scavenger of the present invention, or can be made into a concentrated / dried dosage form. When obtaining a dried product, a carrier such as dextrin or cyclodextrin may be added to improve hygroscopicity.

The rapeseed extract or extract obtained as described above has no unpleasant odor and does not have the color tone peculiar to the extract, and therefore can be used as it is as an active oxygen scavenger. In that case, it may be purified for the purpose of improving the active oxygen scavenging action, decoloring and deodorizing, or may be mixed with any auxiliary agent to prepare a formulation.

The active oxygen scavenger of the present invention should be incorporated into various cosmetics such as ointments, creams, emulsions, lotions, packs, lips, bath salts, hair tonics, hair lotions, soaps, body shampoos, and then absorbed. This can serve to enhance the active oxygen scavenging action.

In this case, the preferable blending ratio of the active oxygen scavenger of the present invention with respect to the cosmetic material excellent in preventing the generation of active oxygen is about 0 in terms of a dry product when it comprises a standard unpurified extract. It is 0.0001% by mass to 10% by mass.

In the cosmetic of the present invention, in addition to the active oxygen scavenger, astringents, bactericides / antibacterial agents, ultraviolet absorbers, humectants, cell activators, and anti-inflammatory agents, which are various optional ingredients usually used in cosmetics.・ Anti-allergic agent, anti-oxidant / active oxygen remover, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, etc. may be appropriately selected and blended according to the purpose. .

The active oxygen scavenger of the present invention can be used by blending it with general foods, health foods, health functional foods, quasi-drugs and pharmaceuticals which are orally ingested. In this case, the added amount of the active oxygen removing agent of the present invention is
Although it varies depending on the food or drink to be added and cannot be specified unconditionally, it is usually about 0.01 to 50% by mass. Considering the general intake of foods and drinks to be added, the intake of rapeseed extract per day for an adult is about 1 mg to 1000 m per day.
It is preferable to adjust it to about g.

Foods and drinks to which the active oxygen remover of the present invention can be applied are not particularly limited, and examples thereof include soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks and the like; ice cream, ice sorbet, shaved ice. Frozen desserts such as; buckwheat noodles such as noodles such as noodles such as noodles, candy, gum, chocolate, confectionery, snacks, biscuits,
Confectionery such as jelly, jam, cream, baked goods, bread, etc .; crab, salmon, clam, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, red snail, scallop, abalone, sea urchin, salmon roe, tocobushi And other marine products; processed fish and livestock products such as kamaboko, ham, and sausage; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressings, and other processed fats and oils; Source,
Seasonings such as sauces; curry, stew, oyakodon, porridge, porridge, Chinese rice bowl, katsudon, tempura bowl, unadon, hayashi rice, oden, marbo dofu, beef bowl, meat sauce, egg soup, omelet rice, dumplings, chouxmai, hamburg, Retort pouch foods such as meatballs; various forms of health
Dietary supplements: pharmaceuticals such as tablets, capsules, drinks, troches, and quasi-drugs, and the like, which can be added together with supplementary raw materials and additives usually used in the production of these.

[0034]

EXAMPLES The present invention will be specifically described below with reference to production examples and examples, but the present invention is not limited to the following examples.

[Production Example 1] Rape blossoms (Brassica)
300 g of crushed material obtained by crushing the above ground part of
2000 mL of water, 80% by mass of ethanol, or ethanol was added to the mixture, and the mixture was gently stirred for 3 hours while maintaining the temperature at 70 ° C., and then filtered using a filter paper to obtain an extract. After concentrating this extract under reduced pressure at 40 ° C., it was dried with a vacuum dryer to obtain an extract dried product. The yield of each of the obtained extracts is shown in Table 1.

[0036]

[Table 1]

[Example 1] Superoxide erasing test
Test 3 mM xanthine, 0.05 M Na ₂ CO ₃ buffer (p
H10.2), 3 mM EDTA, BSA solution, and 0.
Each of 75 mM NBT was placed in a 0.1 mL test tube, and 0.1 mL of each sample solution of Production Example 1 was added thereto, and the temperature was adjusted to 25 ° C.
Left for 10 minutes. Then, a xanthine oxidase solution was added, the mixture was stirred rapidly, and the mixture was allowed to stand at 25 ° C. for 20 minutes. Then, 0.1 mL of 6 mM copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured.

The same operation and measurement of absorbance were carried out without adding the enzyme solution. Furthermore, the same measurement was performed when distilled water was added without adding the sample solution. The superoxide scavenging rate (%) was calculated from the obtained results by the following mathematical formula (1).

<Equation 1> Erasure rate (%) = {1- (AB) / (CD)} × 10
0 where A is the absorbance when the enzyme solution is added and the sample solution is added B: the absorbance when the enzyme solution is not added, the absorbance when the sample solution is added C: the absorbance when the enzyme solution is added and the sample solution is not added D: the enzyme solution is not added, the sample solution Absorbance without addition

Next, the sample concentration was gradually reduced to measure the superoxide scavenging rate, and the sample concentration (ppm) at which the superoxide scavenging rate was 50% was determined by the interpolation method. The results are shown in Table 2.

[0041]

[Table 2]

Example 2 Hydrogen Peroxide Elimination Test A standard solution of hydrogen peroxide (concentration 1.5 mM) 10 μL was added,
After adding 10 μL of each sample solution of Production Example 1 and incubating at 37 ° C. for 20 minutes, the coloring reagent [DA-64
(Wako Pure Chemical Industries) 10 mM, Triton X-100 0.
1 mL of peroxidase solution (100 unit / mL, Wako Pure Chemical Industries, Ltd.) was added to 0.1 M PIPES buffer solution (pH 7.0) containing 5%, and the total amount was adjusted to 100 mL]
After adding 2.98 mL and incubating at 37 degreeC for 5 minutes, the light absorbency in wavelength 727nm was measured.

The same operation and absorbance measurement were carried out without adding a standard solution of hydrogen peroxide. Further, the same measurement was performed in the case where distilled water was added without adding the sample solution, and the elimination rate of hydrogen peroxide was determined by the following mathematical formula (2).

<Equation 2> Erasure rate (%) = {1− (A−B) / (C−D)} × 100 where A: Absorbance when adding hydrogen peroxide standard solution and sample solution B: Excess Absorbance without addition of hydrogen oxide standard solution, sample solution C: Absorbance without addition of hydrogen peroxide standard solution, sample solution D: Absorbance without addition of hydrogen peroxide standard solution, sample solution

Next, the hydrogen peroxide erasing rate was measured by gradually reducing the sample concentration, and the hydrogen peroxide erasing rate was 5%.
The sample concentration (ppm) at 0% was determined by the interpolation method.
The results are shown in Table 3.

[0046]

[Table 3]

Example 3 Radical scavenging test 1.5 × 10 ⁻⁴ M DPPH ethanol solution 3 mL
3 mL of each sample solution of Production Example 1 was immediately added, the container was immediately sealed and shaken, and the mixture was allowed to stand for 30 minutes.
The absorbance at m was measured.

As a control, a solvent in which the sample solution was dissolved was used in place of the sample solution, and the same operation was performed.
Absorbance at 20 nm was measured. Also, as a blank
Immediately after adding 3 mL of the sample solution to ethanol,
Absorbance at 20 nm was measured. From each measured absorbance, the radical scavenging rate (%) was calculated by the following mathematical formula (3).

<Equation 3> Erasure rate (%) = {1− (B−C) / A} × 100 However, A: absorbance of control B: Absorbance when sample solution was added C: Absorbance of blank

Next, the radical scavenging rate was measured to be 50 when the radical scavenging rate was measured by gradually reducing the sample concentration.
The sample concentration (ppm) which becomes% was calculated by the interpolation method. The results are shown in Table 4.

[0051]

[Table 4]

Example 4 Emulsion An emulsion having the following composition having an active oxygen removing action was produced by a conventional method. Jojoba oil 4.0 g Placenta extract 0.1 g Magnesium ascorbyl phosphate 0.1 g Prune extract 1.0 g Tonin extract 1.0 g Kyounin extract 1.0 g Taiou extract 0.5 g Kujin extract 0.5 g Olive oil 2.0 g squalane 2.0 g cetanol 2.0 g glyceryl monostearate 2.0 g polyoxyethylene cetyl ether (20 EO) 2.5 g polyoxyethylene sorbitan oleate (20 EO) 2.0 g 1.3 -Butylene glycol 3.0 g Hinokitiol 0.15 g Perfume 0.15 g Rape water extract 10.0 g Purified water balance (total amount is 100 g)

Example 5 Cream A cream having the following composition having an activity of removing active oxygen was prepared by a conventional method. Liquid paraffin 5.0 g Salix beeswax 4.0 g Cetanol 3.0 g Squalane 10.0 g Lanolin 2.0 g Arbutin 0.1 g Rucinol 0.1 g Kojic acid 0.1 g Yellow mud extract 0.1 g Ginkgo biloba extract 0.1 g Conchiolin 0.1. 1 g Oat extract 0.1 g Stearic acid 1.0 g Polyoxyethylene sorbitan oleate (20 EO) 1.5 g Glyceryl monostearate 3.0 g 1.3-Butylene glycol 6.0 g Methyl paraoxybenzoate 0.05 g Perfume 0.1 g Rape flower 80 mass% ethanol extract 5.0 g Purified water balance (total amount is 100 g)

Example 6 Pack A pack having the following composition having active oxygen removing action was produced by a conventional method. Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Chamomile extract 0.1 g Ellagic acid 0.1 g Oil-soluble licorice extract 0.1 g Seaweed extract 0.1 g Yeast extract 0.1 g Perilla extract 0.1 g Jiuyu extract 0 .1 g linden extract 0.1 g ethyl paraoxybenzoate 0.05 g fragrance 0.05 g rapeseed ethanol extract 3 g purified water balance (total amount is 100 g)

[Example 7] Capsule A capsule having the following composition having an action of removing active oxygen was produced by a conventional method. No. 1 hard gelatin capsule was used as the capsule. Rapeseed water extract 20.0 mg Corn starch 60.0 mg Lactose 100.0 mg Calcium lactate 10.0 mg Hydroxypropyl cellulose (HPC-L) 10.0 mg

[Example 8] Granular preparation A granular preparation having the following composition having an active oxygen removing action was produced by a conventional method. Rape flower 80 mass% ethanol extract 50.0 mg beef bone powder (containing 20% calcium) 250.0 mg egg shell calcium (containing 34% calcium) 147.0 mg soybean extract (with 10% isoflavone) 25.0 mg Vitamin B mixed powder (total) Vitamin B included) 100.0 mg Fat-soluble vitamin mixed powder (including vitamins D, K, E, A) 100.0 mg Milk protein hydrolyzate (including amino acids and low molecular weight peptides) 50.0 mg Corn, soy protein hydrolyzed Degradation products (including amino acids and peptides) 20.0 mg Wheat germ extract (including vitamins, chromium, selenium, and molybdenum) 30.0 mg Yeast extract (including vitamins, nucleic acids, and zinc) 50.0 mg Erythritol 300.0 mg Lactulose 100 0.0 mg maltitol 168.7m Beet oligosaccharides 100.0mg Chichihate oligosaccharide 100.0mg guar gum 60.0mg glycerin fatty acid ester 15.0mg

Example 9 Oral Liquid Preparation An oral liquid preparation having the following composition having an active oxygen removing action was produced by a conventional method. Rape flower ethanol extract 0.5% by mass Sorbit 12.0% by mass Sodium benzoate 0.1% by mass Perfume 1.0% by mass Calcium sulfate 0.5% by mass Purified water balance (total amount is 100% by mass)

[0058]

As described above, according to the present invention,
A natural active oxygen scavenger that has an excellent active oxygen scavenging action based on the superoxide scavenging action, hydrogen peroxide scavenging action and radical scavenging action, is highly safe and is easily available as a raw material.

By adding the active oxygen scavenger of the present invention to cosmetics and foods and drinks, it is possible to impart an excellent active oxygen scavenging action based on the superoxide scavenging action, hydrogen peroxide scavenging action and radical scavenging action. can do.

Front page continuation (51) Int.Cl. ⁷ identification code FI theme code (reference) A61K 7/48 A61K 7/48 A61P 17/16 A61P 17/16 29/00 29/00 31/12 31/12 35 / 00 35/00 43/00 111 43/00 111 (72) Inventor Zhou Yanyang 14703-10 Mutocho, Onomichi, Hiroshima Prefecture Maruzen Pharmaceutical Co., Ltd. F-term (reference) 4B018 MD53 ME06 4C083 AA072 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC182 AC242 AC422 AC442 AC482 AC842 AD042 AD112 AD392 AD512 AD552 AD642 CC05 CC07 DD31 EE12 EE13 4C088 AB15 AC02 AC03 AC04 AC05 BA08 BA09 CA04 CA05 MA37 MA41 MA63 NA14 ZA89 ZB11 ZB26 ZB33 ZC02

Claims (6)

[Claims] 1. Brassica (Brassic)
a) An active oxygen scavenger containing a superoxide scavenging substance extracted from a genus plant as an active ingredient. 2. Brassicaceae (Brassic)
a) An active oxygen scavenger comprising a hydrogen peroxide scavenging substance extracted from a genus plant as an active ingredient. 3. Brassicaceae Brassica (Brassic)
a) An active oxygen scavenger comprising a radical scavenging substance extracted from a genus plant as an active ingredient. 4. Rape blossoms (Brassica ropa)
4. The activity according to any one of claims 1 to 3, which is obtained by extracting flower parts, seeds, leaves, stems or above-ground parts containing these with water, a hydrophilic organic solvent or a mixed solvent thereof. Oxygen scavenger. 5. A cosmetic comprising the active oxygen scavenger according to any one of claims 1 to 4. 6. A food or drink comprising the active oxygen scavenger according to any one of claims 1 to 4 blended therein.