JP2005082531A - Antiinflammatory agent, cosmetic, and food and beverage - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an antiinflammatory agent having excellent hyaluronidase-inhibiting action and platelet aggregation-inhibiting action, to prepare a cosmetic and to provide a food and a beverage, in which the antiinflammatory agent is formulated, having high skin disease-improving effect and chaped skin-improving effect. <P>SOLUTION: The antiinflammatory agent comprises an extract of Thamnolia vermic ularis Ach. (whiteworm lichen) of the family Usneaceae as an active ingredient. The antiinflammatory agent comprises, preferably, at least one substance selected from a hyaluronidase inhibitor and platelet aggregation inhibitor. The cosmetic is obtained by formulating the antiinflammatory. The food or the beverage is obtained by formulating the antiinflammatory. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an anti-inflammatory agent having an excellent hyaluronidase inhibitory action and platelet aggregation inhibitory action, and a cosmetic and a food and drink that have a high effect of improving skin diseases and rough skin containing the anti-inflammatory agent.

  There are various causes and onset mechanisms of inflammatory diseases such as contact dermatitis (rash), psoriasis, pemphigus vulgaris, and various other skin diseases associated with rough skin. The causes are due to hyaluronidase and platelet aggregation. Things are known.

The hyaluronidase is an enzyme that hydrolyzes hyaluronic acid, but hyaluronic acid is a mucopolysaccharide found in the mesenchymal tissue and serves to prevent invasion and transmission of microorganisms and toxicants. When activated, the damage caused by the decrease in the protective effect of hyaluronic acid is likely to occur. Hyaluronidase is also related to type I allergic reaction, and is considered to be an enzyme that governs degranulation from mast cells. Therefore, the hyaluronic acid hydrolyzing enzyme, hyaluronidase, is inhibited, thereby stabilizing the hyaluronic acid and preventing the release of various chemical mediators from mast cells. it can.
Therefore, attempts have been made to extract substances having such a hyaluronidase inhibitory activity from natural products. For example, extracts of plants belonging to the genus Osbeckia (see Patent Document 1), Fuji tea extract (see Patent Document 2). Rosemary, Thyme and Melissa extract (see Patent Document 3) have been reported.

On the other hand, platelet aggregation leads to activation of phospholipase A _{2 in} the rachidonic acid cascade, whereby leukotriene B, prostaglandin E _{2 and the} like are released and become a flame-inducing substance. Therefore, it has been attempted to prevent and treat allergic diseases and inflammatory diseases by extracting substances that inhibit and suppress platelet aggregation from natural products. For example, an extract of a plant belonging to the genus Canalium (refer to Patent Document 4), an extract of Kusasanfu (refer to Patent Document 5), an extract of Fuji tea (refer to Patent Document 6), and the like have been reported.

  However, there is a strong demand from consumers for natural anti-inflammatory agents having high hyaluronidase inhibitory activity and platelet aggregation inhibitory activity while being excellent in safety and productivity, and inexpensive, and those that can still be sufficiently satisfied. It is currently not provided.

Japanese Patent Laid-Open No. 2003-55242 JP 2003-12532 A Japanese Patent Laid-Open No. 08-333267 JP 2002-53478 A JP 2002-53477 A JP 2001-97873 A

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention has an excellent hyaluronidase inhibitory action and platelet aggregation inhibitory action, a highly safe anti-inflammatory agent that can prevent and ameliorate various inflammatory diseases, and skin diseases containing the anti-inflammatory agent, An object of the present invention is to provide cosmetics and foods and drinks that are excellent in improving and preventing troubles of the scalp and skin such as rough skin.

  As a result of intensive studies by the present inventors in order to solve the above problems, the following knowledge was obtained. That is, the extract of snow tea of Musioke department lichen has an excellent hyaluronidase inhibitory action and platelet aggregation inhibitory action, and is useful as an anti-inflammatory agent capable of preventing and improving various inflammatory diseases such as skin diseases and rough skin. It is knowledge.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
<1> An anti-inflammatory agent characterized by containing an extract of Snow Tea (Thamnolia vermicularis Ach.), Which is a lichen of the moss family.
<2> The anti-inflammatory agent according to <1> above, wherein the extract of the moss mosquito lichen snow tea (Thamnolia vermicularis Ach.) Includes at least one selected from a hyaluronidase inhibitor and a platelet aggregation inhibitor. .
<3> From <1> obtained by extracting the whole plant of Snow Tea (Tamnolia vermicularis Ach.) From any one selected from water, a hydrophilic organic solvent and a mixed solvent thereof. 2>.
<4> A cosmetic comprising the anti-inflammatory agent according to any one of <1> to <3>.
<5> A food or drink comprising the anti-inflammatory agent according to any one of <1> to <3>.

  According to the present invention, various problems in the prior art are solved, and through the high hyaluronidase inhibitory action and platelet aggregation inhibitory action of the extract of snow tea of Musgomaceae lichen, these involved contact dermatitis (rash), psoriasis, It is possible to effectively prevent and ameliorate pemphigus vulgaris and other inflammatory diseases associated with rough skin, and provide cosmetics and foods and drinks having an effect of preventing skin diseases and rough skin.

(Anti-inflammatory agent)
The anti-inflammatory agent of the present invention contains an extract of the moss L. lichen snow tea (Thamnolia vermicularis Ach.), And further contains other components as necessary.
Here, the snow tea extract of the moss mosquito lichen has high hyaluronidase inhibitory action and platelet aggregation inhibitory action, and is useful as an anti-inflammatory agent that can prevent and ameliorate various inflammatory diseases such as skin diseases and rough skin. There is no known at all about these, and these are new findings based on the diligent research of the present inventors.

It is preferable that the snow tea extract of Musigoceae lichen contains at least one of a hyaluronidase inhibitor and a platelet aggregation inhibitor.
The snow tea (Thamnolia vermicularis Ach.) Belongs to the symbiotic lichen which is a symbiosis composed of certain fungi and algae. Widely distributed and easily available. It should be noted that the snow tea of the moss lichen used in the present invention may be artificially produced by tissue culture.

  Although it is not yet confirmed what substances contained in the snow tea of the moss moss lichen and exhibit the hyaluronidase inhibitory action and the platelet aggregation inhibitory action, In addition, it can be easily extracted by adding it to any one of the hydrophilic organic solvent and the mixed solvent thereof and extracting it at any temperature from room temperature to the boiling point of the solvent.

  There is no restriction | limiting in particular as said extraction raw material, Although it can select suitably according to the objective, The whole plant of snow tea of Musigoceae lichen is preferable. It is desirable that the whole plant of snow tea of the moss mosquito lichen as the extraction raw material is used after dry pulverization or coarse pulverization, and may be used after degreasing with a nonpolar solvent such as hexane.

Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. These may be used alone or in combination of two or more.
Examples of the treatment applied to the water include purification, heating, sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, and buffering. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, propylene glycol, Examples thereof include C 2-5 polyhydric alcohols such as glycerin, and these may be used alone or in combination of two or more.
When a mixed solvent of water and a hydrophilic organic solvent is used, when a lower alcohol is used as the hydrophilic organic solvent, the mass ratio of the water to the lower alcohol is 10: 1 to 10: It is preferable to mix so that it may become 90. When a lower aliphatic ketone is used as the hydrophilic organic solvent, it is preferable to mix so that the mass ratio of the water and the lower aliphatic ketone is 10: 1 to 10:40. When a polyhydric alcohol is used as the hydrophilic organic solvent, the mass ratio of the water and the polyhydric alcohol is preferably 10: 1 to 10:90.

As a method for extracting snow tea from the mosquito family lichen, for example, a dried and pulverized product of snow tea leaves is put into a processing tank filled with an extraction solvent, and appropriately stirred for 30 minutes. After standing for ˜4 hours to elute soluble components, the solids are removed by filtration or centrifugation, and when the extraction solvent is distilled off from the resulting extract, a paste-like concentrate is obtained. Is dried to obtain a solid extract.
However, the snow tea extract does not have to be a solid extract, and may be in the state of the extract or its concentrated solution. These may be used after being purified by a method such as activated carbon treatment, adsorption resin treatment, ion exchange resin, liquid-liquid countercurrent distribution, etc., if the object and effects of the present invention are not disturbed.

  In addition, it is preferable that the amount of said extraction solvent is 5-15 times amount (mass ratio) normally with respect to snow tea of Musgogidae lichen as said extraction raw material. As the extraction conditions, when water is used as the extraction solvent, it is usually preferably about 50 to 95 ° C. for about 1 to 4 hours. When the mixed solvent of water and ethanol is used as the extraction solvent, it is usually It is preferable that it is about 30 minutes-4 hours at 40-80 degreeC.

  The resulting extract of snow tea of Musigoceae lichen or an appropriately purified product thereof can be used as it is as the anti-inflammatory agent of the present invention, but can also be made into a concentrated and dried dosage form. In obtaining a dried product, a carrier such as dextrin or cyclodextrin may be added to improve hygroscopicity.

  The extract or extract of snow tea of Musigoceae lichen obtained as described above has no unpleasant odor and does not have a color tone peculiar to the extract, and thus can be used as it is as an anti-inflammatory agent. If necessary, it may be purified for the purpose of improving the activity or decoloring / deodorizing, or may be mixed with an optional auxiliary agent.

Since it has been confirmed that the anti-inflammatory agent of the present invention is not digested in the digestive tract, it is suitable for blending into any food or beverage supplement or dietary supplement.
In addition, the anti-inflammatory agent of the present invention has high hyaluronidase inhibitory action and platelet aggregation inhibitory action, has high safety, and can be suitably used for the following cosmetics or foods and drinks of the present invention.

(Cosmetics)
The cosmetic of the present invention comprises the anti-inflammatory agent of the present invention, and further comprises other components as necessary.

  Examples of the cosmetics include ointments, creams, milky lotions, lotions, packs, lips, bath preparations, hair nicks, hair lotions, soaps, body shampoos, and the like. By blending the anti-inflammatory agent of the present invention into the cosmetic and absorbing it to the skin, scalp and the like, it can be used to enhance the hyaluronidase inhibitory action and the platelet aggregation inhibitory action.

  As a suitable blending ratio of the anti-inflammatory agent of the present invention with respect to the cosmetic, in the case of a snow tea extract of unpurified standard Musigoceae lichen, about 0.0001 to 10 mass in terms of dry matter %.

  If necessary, the cosmetics can use various main ingredients and auxiliaries usually used in the production of the cosmetics and other components as long as the effects of the present invention are not impaired.

  The other components are not particularly limited as long as they do not interfere with the hyaluronidase inhibitory action and the platelet aggregation inhibitory action, and include ingredients appropriately selected according to the purpose. Examples include whitening agents, astringents, bactericidal / antibacterial agents. , UV absorbers, moisturizers, cell activators, anti-inflammatory / anti-allergic agents, antioxidant / active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, perfumes , Etc. These components, when used in combination with the snow tea extract of the moss mosquito lichen, may act synergistically to provide a superior use effect than would normally be expected.

  Examples of the whitening agent include ascorbic acid or a derivative thereof, sulfur, placental hydrolyzate, ellagic acid or a derivative thereof, kojic acid or a derivative thereof, glucosamine or a derivative thereof, arbutin or a derivative thereof, hydroxycinnamic acid or a derivative thereof, Glutathione, Arnica extract, Ogon extract, Sowakuhihi extract, Psycho extract, Bowfish extract, Candida mycelium culture or extract thereof, Linden extract, Peach leaf extract, Ages extract, Kujin extract, Jiuyu extract, Toki extract, Yakuinin extract, Oyster leaf extract , Dianthus extract, Buttonpi extract, Camelis extract, Maronnier extract, Hypericum extract, Oil-soluble Licorice extract (Liquorice flavone, Grabrizine, Grabrene, Lycochalcone A), etc. The may be used alone or in combination of two or more thereof. Among these, from the viewpoint of improving the whitening effect, it is preferable to use at least one selected from ascorbic acid or a derivative thereof, placenta extract, chamomile extract, arbutin, ellagic acid, lucinol and kojic acid.

  Examples of the astringent include, for example, citric acid or salts thereof, tartaric acid or salts thereof, lactic acid or salts thereof, aluminum chloride, aluminum sulfate / potassium, allantoinchlorohydroxyaluminum, allantoindihydroxyaluminum, zinc paraphenolsulfonate, zinc sulfate, Examples include jelly extract, age extract, hamamelis extract, genus pepper extract, tea catechins, adriatic extract, hypericum extract, dairy extract, cornflower extract, kizuta extract, cucumber extract, maronier extract, salvia extract, and melissa extract. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the bactericidal / antibacterial agent include benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, benzethonium chloride, chlorhexidine hydrochloride, photosensitizer 101, photosensitizer 201, salicylic acid, sodium salicylate, sorbine Acid, halocarban, resorcinol, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, chitosan degradation product, jellyfish extract, kudin extract, enmisodia extract, loquat extract, yucca extract, aloe extract, cinnamon extract, gadget extract, etc. It is done. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the ultraviolet absorber include β-isopropylfuranone derivatives, urocanic acid, ethyl urocanate, oxybenzone, oxybenzonesulfonic acid, tetrahydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, synoxate, methyl diisopropylcinnamate, octyl methoxycinnamate Glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, titanium oxide, β-carotene, γ-oryzanol, rice bran extract, aloe extract, birch extract, birch extract, Chamomile extract, henna extract, butterfly extract, ginkgo biloba extract, chamomile extract, hawthorn extract, Soluble licorice extract, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the humectant include serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, glyceroglycolipid, sphingophospholipid, sphingosaccharide Lipids, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium culture or extract thereof, wheat germ oil, avocado oil, rice Germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, yokuinin extract, siamese extract, typhoid extract, diatomaceous earth extract, beetle aloe extract, burdock extract, mannen wax extract, arnica extract, wheat Suma, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the cell activator include riboflavin or derivatives thereof, pyridoxine or derivatives thereof, nicotinic acid or derivatives thereof, pantothenic acid or derivatives thereof, α-tocopherol or derivatives thereof, arnica extract, carrot extract, rapeseed extract, loofah extract (Saponin), shikon extract, buckwheat extract, button pea extract, peony extract, mugwort extract, safflower extract, ashitaba extract, loquat leaf extract, shrimp extract, saxifrage extract, jaundice extract, salvia extract, garlic extract, mannen wax extract, etc. It is done. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the anti-inflammatory / antiallergic agent include azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or a derivative thereof, glycyrrhetinic acid or a derivative thereof, photosensitive element 301, photosensitive element 401 , Diphenhydramine hydrochloride, tranexamic acid or derivatives thereof, adenosine phosphate, estradiol, eslon, ethinyl estradiol, cortisone, hydrocortisone, prednisone, progesterone, corticosterone, arnica extract, inchinko extract, sanshishi extract, citrus extract, licorice extract, toki Extract, Artemisia Extract, Walnut Extract, Gentian Extract, Psycho Extract, Senkyu Extract, Achillea Millefolium Extract Coptis extract, mint extract, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the antioxidant / active oxygen scavenger include, for example, dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, rosemary extract, melissa extract, orgon extract, age extract, Loquat leaf extract, hop extract, clam extract, peony extract, sage extract, kina extract, chamomile extract, eucalyptus extract, perilla extract, ginkgo biloba extract, thyme extract, cardamom extract, caraway extract, nutmeg extract, mace extract, laurel extract, clove extract , Turmeric extract, willow extract, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the fats and oils include soybean oil, linseed oil, sesame oil, nutka oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, camellia oil, almond oil, castor oil, peanut oil, cacao oil, palm kernel Oil, beef tallow, mink oil, jojoba oil, evening primrose oil, horse oil, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the waxes include carnauba wax, candelilla wax, beeswax, white beeswax, whale wax, cerax, lanolins, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the hydrocarbons include liquid paraffin, petrolatum, microlistin wax, ceresin, squalane, and polyethylene powder. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the fatty acids include stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hebenic acid, lanolinic acid, oleic acid, undecylenic acid, and isostearic acid. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol, oleyl alcohol, hexadecyl alcohol, 2-octyldodecanol, glycerin, sorbitol, propylene glycol, and 1,3-butylene. Examples include glycol, ethylene glycol or a polymer thereof, glucose, sucrose, cholesterol, phytosterol, cetostearyl alcohol, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the esters include decyl oleate, butyl stearate, myristyl myristate, hexyl laurate, isopropyl palmitate, isopropyl myristate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, propylene glycol dioleate, phthalic acid Examples include diethyl, glyceryl monostearate, glyceryl trimyristate, and cetyl lactate. These may be used individually by 1 type and may use 2 or more types together.

  As the surfactant, for example, a nonionic surfactant, an anionic surfactant, a cationic surfactant, an amphoteric surfactant, and the like can be used. Alternatively, surfactants listed in minor cosmetic raw material standards are preferable, for example, soybean lecithin, egg yolk lecithin, saponin, oligoglycoside, phospholipid biosurfactant, acyl peptide biosurfactant, polyoxyethylene polyoxypropylene glycol , Polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene butyl ether, polyoxyethylene coconut oil fatty acid monoethanolamide, polyoxyethylene lauryl ether, polyoxyethylene lauryl ether sulfate triethanol Amine, sodium polyoxyethylene lauryl ether sulfate, polyoxyethylene lauryl ether phosphate, polyoxyethylene lauryl ether sodium phosphate, polyoxyethylene lanolin, polyoxyethylene lanolin alcohol, polyoxypropylene butyl ether, polyvinyl alcohol, polyvinyl pyrrolidone, polyphosphorus Acid sodium, sorbitan monooleate, polyethylene glycol monooleate, polyoxyethylene sorbitan monooleate, ethylene glycol monostearate, sorbitan monostearate, propylene glycol monostearate, polyethylene glycol monostearate, polyoxymonostearate Ethylene glycerol, polyoxyethylene sorbitan monostearate, Sorbitan palmitate, monopalmitate polyoxyethylene sorbitan, sorbitan monolaurate, polyethylene glycol monolaurate, polyoxyethylene sorbitan monolaurate, polyoxyethylene monolaurate sorbitol, coconut oil fatty acid diethanolamide, and the like. These may be used individually by 1 type and may use 2 or more types together.

  Examples of the perfume include menthol, carvone, eugenol, anethole, mint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil, and the like. These may be used individually by 1 type and may use 2 or more types together.

  The cosmetic of the present invention has high safety especially when used on the skin and scalp, has an excellent hyaluronidase inhibitory action and platelet aggregation inhibitory action, prevention and prevention of inflammation of the skin and scalp, rough skin It is effective for prevention.

(Food)
The food and drink of the present invention is formed by blending the anti-inflammatory agent of the present invention, and further blended with other components as necessary.

  The foods and drinks are those that are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life, such as foods, pharmaceuticals, quasi drugs in administrative divisions, etc. It is not limited to this category, and means, for example, a wide range of foods that are orally ingested, including general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals.

  There is no restriction | limiting in particular as said food-drinks, Although it can select suitably according to the objective, For example, drinks, such as a soft drink, a carbonated drink, a nutrition drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, shaved ice Noodles such as buckwheat noodles, udon, harusame, gyoza skin, Chinese noodles, instant noodles, etc .; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery Confectionery such as bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallops, abalone, sea urchin, crabs, tocobushi, etc .; Fishery and livestock processed foods such as sausages; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, show Oils and processed oils such as ning, whipped cream and dressing; seasonings such as sauces and sauces; curry, stew, oyakodon, rice bowl, miscellaneous foods, Chinese rice bowl, and rice cake, tempura, eel rice cake, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburg, meatballs; various forms of health and nutritional supplements; pharmaceuticals such as tablets, capsules, drinks, troches, etc. Goods. In addition, the said food / beverage is not limited to the said illustration.

  The food and drink of the present invention can be taken orally on a daily basis, and skin diseases, rough skin, and the like can be prevented by the excellent hyaluronidase inhibitory action and platelet aggregation inhibitory action of the anti-inflammatory agent of the present invention.

  The amount of the anti-inflammatory agent of the present invention to be added to the food and drink varies depending on the food and drink to be added and cannot be defined unconditionally, but is usually 0.01 to 50% by mass. Taking into consideration the general intake of food and drink to be added, it is preferable to prepare so that the intake of snow tea extract of Musigoceae lichen per day is about 1 to 1,000 mg.

Examples of the other components include auxiliary raw materials or additives that are usually used in producing the food and drink.
The raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose.For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid, Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, gum arabic , Carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinamide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives, and the like.

  The food and drink of the present invention can be taken orally on a daily basis, and the hyaluronidase inhibitory action and the platelet aggregation inhibitory action are extremely effectively achieved by the action of the snow tea extract of Musigoke family lichen which is an active ingredient. can do.

  In addition, although the anti-inflammatory agent of this invention, cosmetics, and food and drink are suitably applied with respect to a human, as long as each effect is show | played, it applies with respect to animals other than a human. You can also.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

(Production Example 1)
-Manufacture of snow tea water extract-
300 g of dried dried green tea of Mussoke family lichen was added to 3 liters of water, heated and extracted with a reflux extractor at 80 ° C. for 2 hours, and filtered while hot. The same extraction process was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and dried to obtain a snow tea water extract. The yield of the extract was as shown in Table 1.

(Production Example 2)
-Manufacture of snow tea 50 mass% ethanol extract-
300 g of dried dried green tea of Mussoke family lichen was added to 3 liters of 50% by weight ethanol, heated and extracted at 80 ° C. for 2 hours with a reflux extractor, and filtered while hot. The same extraction process was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and dried to obtain a 50% by weight ethanol extract of snow tea. The yield of the extract was as shown in Table 1.

(Production Example 3)
-Manufacture of 80% by weight ethanol extract of snow tea-
300 g of dried dried green tea of Mussoke family lichen was added to 3 liters of 80% by mass ethanol, heated and extracted at 80 ° C. for 2 hours with a reflux extractor, and filtered while hot. The same extraction process was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and dried to obtain an 80% by weight ethanol extract of snow tea. The yield of the extract was as shown in Table 1.

(Example 1)
-Hyaluronidase inhibition test-
The snow tea extract obtained in Production Examples 1 to 3 was tested for hyaluronidase inhibitory action as follows.
After mixing 0.1 mL of a hyaluronidase solution (400 units / mL, pH 3.5 acetate buffer solution) and 0.2 mL of each sample solution of Production Examples 1 to 3, the mixture was heated to 37 ° C. for 20 minutes, and then an activator solution. 0.2 mL of (2.5 mmol / L CaCl ₂ ) was added, and the enzyme was activated by heating at 37 ° C. for 20 minutes. After adding 0.5 mL of potassium hyaluronate buffer and reacting at 37 ° C. for 40 minutes, 0.2 mL of 0.4N sodium hydroxide was added and ice-cooled to stop the reaction.
Next, 0.2 mL of 0.8 mol / L boric acid solution (pH 9.1) was added, heated in a boiling water bath for 3 minutes, and immediately cooled on ice for 20 minutes. p-DABA reagent (10 g of p-dimethylaminobenzaldehyde dissolved in 12.5 mL of 10N hydrochloric acid and 87.5 mL of acetic acid and diluted 10-fold with acetic acid) was added to 6.0 mL and added at 37 ° C. for 20 minutes. Incubated. The N-acetylglucosamine released by the enzyme reaction was colored and the absorbance at a wavelength of 585 nm was measured. The same operation and absorbance measurement were performed without adding the enzyme. Further, as a control, the same operation and absorbance measurement were performed when distilled water was added instead of the sample solution.
From the above measurement results, the hyaluronidase activity inhibition rate (%) was calculated by the following formula.

<Formula 1>
Hyaluronidase activity inhibition rate (%) =
[1- (AB) / (CD)] × 100
However, in the formula, A means absorbance at the time of sample solution addition and enzyme addition. B means the absorbance when the sample solution is added and no enzyme is added. C means the absorbance when no sample is added and when the enzyme is added (control). D means the absorbance when no sample was added and no enzyme was added (control).

Then, the concentration of the sample solution was changed stepwise to measure the hyaluronidase activity inhibition rate, and the sample concentration IC _{50 at} which the inhibition rate was 50% was determined by interpolation (the smaller the IC ₅₀ value, the more hyaluronidase inhibition was observed). Strong action). The results are shown in Table 2.

表２の結果から、雪茶抽出物が高いヒアルロニダーゼ阻害作用を有することが確認できた。

From the results in Table 2, it was confirmed that the snow tea extract has a high hyaluronidase inhibitory action.

(Example 2)
-Platelet aggregation inhibition test-
About the snow tea extract obtained by manufacture example 1-3, the platelet aggregation inhibitory effect was tested as follows.
1/10 volume of 77 mM EDTA was added to the blood of Japanese white rabbit and centrifuged at 1000 rpm for 10 minutes to remove the precipitate. The supernatant was centrifuged at 2100 rpm for 10 minutes, and the precipitated platelets were collected. The obtained platelets were suspended in a platelet washing solution and centrifuged at 2100 rpm for 10 minutes. The precipitated platelets were collected and suspended in a platelet suspension so that the platelet count was 300,000 / μL.
Next, 1 μL of a calcium chloride solution was added to 223 μL of the prepared washed platelet suspension and held at 37 ° C. for 1 minute. 1 microliter of each sample solution of manufacture examples 1-3 was added there, and also it hold | maintained at the same temperature for 2 minutes, Then, it stirred for 1 minute.
Next, 25 μL of a 10 ppm collagen solution was added as an aggregation inducer, incubated for 10 minutes at 37 ° C., and then the platelet aggregation rate A was measured using a platelet aggregation measuring device PAM12CL (manufactured by Mevanics Co., Ltd.). Separately, except that the solvent of the sample solution was not added instead of the sample solution, the platelet aggregation rate B was measured in the same manner as described above, and the platelet aggregation inhibition rate (%) was obtained from the following formula from these results. .

<Formula 2>
Platelet aggregation inhibition rate (%) = [(B−A) / B] × 100
However, in the above formula, A means the platelet aggregation rate at the time of adding the aggregation-inducing agent and adding the sample solution. B means the platelet aggregation rate when the aggregation inducer is added and the sample solution is not added.

Then, the concentration of the sample solution was changed stepwise to measure the platelet aggregation inhibition rate, and the concentration IC _{50 at} which the inhibition rate was 50% was determined by interpolation (the smaller the IC ₅₀ value, the more the platelet aggregation inhibition) Strong action). The results are shown in Table 3.

表３の結果から、雪茶抽出物が優れた血小板凝集抑制作用を有することが確認できた。

From the results in Table 3, it was confirmed that the snow tea extract has an excellent platelet aggregation inhibitory action.

  Next, when the following cosmetics and foods and drinks were produced using the snow tea extract obtained in Production Examples 1 to 3, a good formulation could be obtained in any case.

(Example 3)
-Emulsion-
An emulsion having an anti-inflammatory action having the following composition was produced by a conventional method.
Jojoba oil 4.0g
Placenta extract 0.1g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
2.5 g of polyoxyethylene cetyl ether (20E.O)
Oleic acid polyoxyethylene sorbitan (20E.O) 2.0g
1,3-butylene glycol 3.0 g
Hinokitiol 0.15g
Fragrance 0.05g
Snow tea water extract (Production Example 1) 0.01g
Purified water balance
Total 100g

Example 4
-Cream-
A cream having an anti-inflammatory action having the following composition was produced by a conventional method.
Liquid paraffin 5.0g
Salami beeswax 4.0g
Cetanol 3.0g
Squalane 10.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O) 1.5g
3.0 g glyceryl monostearate
1,3-butylene glycol 6.0 g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Snow tea extract (Production Example 3) 0.1g
Purified water balance
Total 100g

(Example 5)
−Pack−
A pack having the anti-inflammatory action having the following composition was produced by a conventional method.
Polyvinyl alcohol 15g
Polyethylene glycol 3g
7g of propylene glycol
Ethanol 10g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Snow tea extract (Production Example 1) 0.05g
Purified water balance
Total 100g

(Example 6)
-Capsule-
Capsules having an anti-inflammatory effect having the composition shown in Table 4 were produced by a conventional method. As the capsule, No. 1 hard gelatin capsule was used.

(Example 6)
-Capsule-
Capsules having an anti-inflammatory effect having the composition shown in Table 4 were produced by a conventional method. As the capsule, No. 1 hard gelatin capsule was used.

(Example 7)
-Granular preparation-
A granular preparation having an anti-inflammatory action having the composition shown in Table 5 was produced by a conventional method.

(Example 8)
-Oral liquid formulation-
An oral liquid preparation having an anti-inflammatory action having the composition shown in Table 6 was produced by a conventional method.

According to the anti-inflammatory agent of the present invention, through the high hyaluronidase inhibitory action and platelet aggregation inhibitory action of the extract of snow tea of Musigoceae lichen, these involved contact dermatitis (rash), psoriasis, vulgaris It can effectively prevent and ameliorate various skin diseases associated with pemphigus and other rough skin. Moreover, since the cosmetics of this invention are excellent in the usability and safety when applied to the skin and scalp, they are suitably used as skin cosmetics or scalp cosmetics having an effect of preventing skin diseases and rough skin. In addition, the food and drink of the present invention can prevent and / or improve skin diseases and rough skin by daily oral administration.

Claims (5)

  An anti-inflammatory agent characterized by comprising an extract of Musnoke family lichen snow tea (Thamnolia vermicularis Ach.).   The anti-inflammatory agent according to claim 1, wherein the extract of the moss mosquito lichen snow tea (Tamnolia vermicularis Ach.) Comprises at least one selected from a hyaluronidase inhibitor and a platelet aggregation inhibitor.   The whole plant of the moss mosquito lichen snow tea (Thamnolia vermicularis Ach.) Is obtained by extraction with any one selected from water, a hydrophilic organic solvent and a mixed solvent thereof. The anti-inflammatory agent described.   A cosmetic comprising the anti-inflammatory agent according to any one of claims 1 to 3. A food or drink comprising the anti-inflammatory agent according to any one of claims 1 to 3.