JP2001181632A - Antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidant having strong anti-oxidizing effect, high safety to the human body and a wide use. SOLUTION: This antioxidant comprises an extract of an olive plant (except leaf, hereinafter the same) as an active ingredient. The extract can be obtained by extracting an olive plant and/or a product obtained in an olive oil production process with water and/or an organic solvent. Further, the extract is concentrated and/or fractioned/purified to improve the effect. Especially, the extract has an anti-oxidizing effect such as preservation stability improving effect and anti-oxidizing effect in vivo such as prevention of various diseases and anti-aging effect.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an olive (Ole).
a europaea L .; ) With respect to antioxidants containing extracts obtained from plants, they have excellent antioxidant effects, especially excellent antioxidant effects such as improving storage stability of products, and various disease preventing effects and antiaging effects. The present invention relates to an antioxidant having an antioxidant effect in a living body.

[0002]

2. Description of the Related Art Fats and oils, particularly fats and oils containing unsaturated fatty acids, are easily oxidized, and are the major causes of deterioration in quality and nutritional and functional properties. Among unsaturated fatty acids, linoleic acid, linolenic acid and arachidonic acid play an important role as essential fatty acids as nutrients. In recent years, EPA,
Attention has been paid to the physiological activities of polyunsaturated fatty acids such as DHA, and many foods and drinks containing these have been put on the market. However, since these unsaturated fatty acids have extremely low oxidative stability, it is necessary to add an antioxidant. However, at present, there is no antioxidant effective for long-term storage.

[0003] Living organisms efficiently obtain the energy necessary for survival by utilizing oxygen. However, in the process of converting oxygen into water in such energy metabolism, reactive oxygen species are generated as an intermediate. In general, as the reactive oxygen species, superoxide released by stimulation of macrophages and the like, and hydroxyl radical generated by radiation exposure and the like are known. These reactive oxygen species are generated due to external triggers such as excessive radiation or ultraviolet irradiation, intake of chemical substances and tobacco, and internal factors such as ischemia reperfusion, inflammation, stress, and aging. Reactive oxygen species generated in excess in the living body in this way generally have high chemical reactivity, easily react with neighboring components such as lipids, nucleic acids, and proteins in the living body, resulting in oxidation that leads to various diseases. Cause disability. Superoxide, a type of reactive oxygen species, has been shown to be closely related to various diseases. For example, LDL in arteries is oxidized by superoxide to form foam cells, which are the causes of arteriosclerosis. Occurs. In addition, the production of hydroxyl radicals caused by irradiation with radiation causes serious obstacles such as carcinogenesis to living organisms. (Hal
liwell B. and Gutteridge
M. C. Biochem. J. 219, 1-1
4, (1984)).

[0004] As the toxicity of such active oxygen species to living organisms becomes apparent, antioxidants such as active oxygen species eliminating substances having the activity of efficiently eliminating them are used in vivo or in foods, pharmaceuticals, agricultural chemicals and the like. Is useful as a protective agent against the oxidative degradation of components contained in foodstuffs, and is expected to have practical use in the food industry, especially processed fishery products, health foods, nutritional foods, as well as in the fields of pharmaceuticals, agricultural chemicals, and cosmetics It is.

[0005] In recent years, consumers are becoming more conscious of the safety issues of food additives made of chemically synthesized products, not limited to antioxidants, such as BHA (butylhydroxyanisole) and BHT (butylhydroxytoluene). And the like, the use of chemically synthesized antioxidants tends to be avoided. Many other antioxidants are oil-soluble (water-insoluble) such as tocopherols derived from vegetable oils, and are often restricted in actual use.

[0006] Under such circumstances, various antioxidants have been extracted mainly from raw materials derived from natural products, and their applications have been studied. For example, superoxide dismutase (SOD), which is an enzyme protein, having superoxide scavenging activity, and mannitol, tryptophan, formic acid, etc., having hydroxyl radical scavenging activity are mentioned (for example, Yoshihiko Oyanagi, "SOD and activity"). Oxygen Species Regulator-Its Pharmacological Action and Clinical Application ", 224
228 pages, The Japan Medical Museum, 1989).

However, since SOD is an enzyme protein, it has poor stability against heat and the like, and when administered orally, most of the administered enzyme is digested and excreted, and its effectiveness is extremely low. At present, there are few practical hydroxyl radical scavengers capable of efficiently scavenging hydroxy radicals. Therefore, at present, it is difficult to industrially obtain these antioxidants in large quantities and stably. Usually, the above-mentioned SOD has an erasing effect only on superoxide and has no effect on hydroxy radicals. Similarly, mannitol cannot scavenge superoxide.

As a raw material having a strong antioxidant effect derived from natural products, which is strongly demanded by consumers, for example, as a water-soluble antioxidant component in sesame seeds, a water-soluble extract obtained from sesame defatted lees or the like is used. It is known that it has antioxidant activity (JP-B-61-26342). It is well known that this defatted lees extract contains lignan glycosides, and it is known that the lignan glycosides have strong hydroxy radical scavenging activity (Japanese Patent Application Laid-Open No. -208686, etc.). However, lignan glycosides contained in sesame seeds and the like cannot be said to have a strong scavenging activity against superoxide.

[0009] In such a situation, it is necessary to stabilize an active ingredient derived from a natural product having not only an erasing activity for one kind of active oxygen species but also a strong erasing activity for plural kinds of active oxygen species such as superoxide and hydroxyl radical. Despite the desire for supply, there have been few examples that have been commercialized industrially. Moreover, it can be said that there is almost no supply of active ingredients having both activities, and stable supply of these is expected.

[0010] On the other hand, one of the plants with a rich dietary experience since ancient times is the olive family belonging to the Oleaceae family and the olives. Olives have been cultivated since ancient times, and the Mediterranean coast is now a typical growing area. It is especially useful as olive oil, and is used not only in Europe, but also in Japan, the United States, and other countries around the world. Olive oil is known to have various effects, and has been used empirically as a medicinal or cosmetic agent since ancient times.
Olive fruits are also used for food as they are, and are sometimes salted as preserved foods. From this, it can be said that olives are stably available and highly safe plant materials for the human body. However, olive oil cake remaining after squeezing olive oil has only been used for fertilizer and fuel.

In recent years, olive oil is known to be a vegetable oil that is relatively hard to oxidize, and polyphenols, which are trace components contained therein, have been attracting attention, and many studies have been made on its physiological effects and the like. (For example, International Olive Oil
Council, New Food Industry
y, Vol. 34, no. 4, 28-52, 199
2). However, there is currently little known about olives other than olive oil. In particular, it has never been known that an extractive component in an olive plant has a strong active oxygen removing function.

[0012]

The present invention provides an excellent antioxidant effect, especially an excellent antioxidant effect such as an effect of improving the storage stability of a product, and an excellent antioxidant effect such as an effect of preventing various diseases and an antiaging effect. It has an antioxidant effect in the body, is stable, has high safety to the human body, is versatile, and aims to provide an antioxidant containing a novel natural extract as an active ingredient, particularly an antioxidant. And an in vivo antioxidant.

[0013]

Means for Solving the Problems The present inventors have conducted intensive studies in order to achieve the above object, and as a result, it has been found that extracts obtained from olive plants have an excellent antioxidant effect, particularly an effect of improving the storage stability of products. The present inventors have found that they have an antioxidant effect and have excellent in vivo antioxidant effects such as various disease prevention effects and antiaging effects, and have completed the present invention. That is, the present invention relates to an antioxidant containing an extract obtained from an olive plant (excluding leaves; the same applies hereinafter) as an active ingredient.
The extract can be obtained by extracting a product obtained in an olive plant and / or olive oil production process with water and / or an organic solvent, and further subjecting the extract to a concentration treatment and / or a fractionation / purification treatment. This enhances the active oxygen removal function of the extract. Also, as the products generated in the olive oil production process, pressed residue, extracted residue, pressed oil, extracted oil, degummed soap, deacidified soap, dark oil, waste decolorizing agent, deodorized scum, pressed juice, The present invention relates to an antioxidant containing an extract obtained from one or more selected from drainage and waste filter media. Since the antioxidant of the present invention is easily soluble in water, it is preferable that the above-mentioned extraction treatment is performed with water or a water-containing organic solvent. In addition, the concentration, fractionation and purification treatment can enhance the active oxygen removing function of the extract by concentrating the water-soluble component, etc., and the antioxidant effect of the antioxidant of the present invention, particularly An excellent antioxidant effect, such as an effect of improving the storage stability of the product, and an excellent antioxidant effect in vivo, such as an effect of preventing various diseases and an antiaging effect, can be achieved. The present invention also relates to an antioxidant containing an extract similarly obtained from olive plants (excluding leaves), and to an antioxidant in vivo. Antioxidants are expected to have a storage stability improving effect, and antioxidants in vivo are expected to have various disease prevention effects and antiaging effects. These antioxidants, antioxidants or in vivo antioxidants are easily soluble in water, and their effects can be suitably achieved by, for example, dissolving a part or the whole in water or a water-containing organic solvent in a liquid state. It can be exerted and can be suitably used. The antioxidant, antioxidant or in vivo antioxidant can be used in various products to improve the antioxidant effect of the product, and to produce foods and beverages having an antioxidant effect in vivo. You can also. In addition, by taking the antioxidant, antioxidant or in vivo antioxidant alone or in a state of being contained in food and drink,
An in vivo antioxidant effect can be obtained. The antioxidant, antioxidant or in vivo antioxidant of the present invention may be used directly for internal use or the like in order to obtain its effect, or may be blended as a raw material for various products. For example, it can be suitably blended in foods and drinks, skin external preparations and the like. The present invention also relates to a method of using an extract obtained from olives as an antioxidant, and more particularly to a method of using an extract obtained from olives as an antioxidant. It relates to a method for use as an agent.

[0014]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail. The present invention relates to an antioxidant comprising, as an active ingredient, an extract obtained from an olive plant, mainly a fruit or a seed, a pericarp, a seed coat, a stem, a bud, and the like, and a dried product, a crushed product, and a defatted product thereof. Oxidant. Since the extract used in the antioxidant of the present invention has a strong active oxygen removing function such as superoxide scavenging activity and hydroxy radical scavenging activity, it is possible to use an antioxidant containing the extract as an active ingredient. In addition, an antioxidant effect such as improvement in storage stability of the product is obtained, and an antioxidant effect in vivo such as an effect of preventing various diseases and an antiaging effect is also obtained. The content of the present extract is not particularly limited, and an appropriate amount of the extract can be contained according to the strength of the effect required by the antioxidant. The extract used for the antioxidant of the present invention is obtained from olive plants, and can be particularly preferably obtained from olive plant fruits and / or seeds, and can be obtained from defatted olive plant fruits and / or seeds. The resulting extract is preferable because it is not necessary to remove unnecessary oil and the like. Also, olive plants produce products in the process of producing olive oil, such as pressed residue, extracted residue, pressed oil, extracted oil, degummed oil residue, deoxidized oil residue, dark oil, waste decolorizer, deodorized scum,
Includes oiled juices, wastewater, and waste filter media. For example, the extract can be obtained not only from oil residue such as a pressing residue or an extraction residue, but also from waste water generated in the oil pressing step of olive oil or olive oil which is usually discarded. It can be said that it is preferable from the viewpoint of effective use of olives. Here, with respect to an antioxidant containing an extract obtained by extracting the above olive plant or the like with water and / or an organic solvent, preferably, the organic solvent is a hydrophilic organic solvent, and further, the hydrophilic organic solvent is used. Preferably, the solvent is an alcohol. According to the present invention, by containing an extract having a strong active oxygen removing function, an antioxidant having an excellent antioxidant effect can be obtained, and the yield of the extract per unit material is also high. . For example, 1 to 10 compared to when using sesame
It can be obtained with double the yield. From both of these facts, the present extract has a significantly higher active oxygen scavenging function yield index, which is obtained by multiplying the strength of the active oxygen scavenging function by the yield per unit material, as compared with the case of using other natural materials. High values are obtained. As for the superoxide scavenging activity, the extract obtained from the unit raw material has a superoxide scavenging activity yield index of 100 or more, approximately 100 to 3250, and for example, a high value of 1 to 650 times as compared with sesame. is there. Therefore, an extract having a strong active oxygen removing function can be obtained with good yield, that is, the antioxidant having an excellent antioxidant effect of the present invention can be obtained at low production cost. Further, the present extract is preferably subjected to concentration treatment and / or fractionation / purification treatment. The concentration, fractionation, purification, and the like improve the active oxygen removal function of the present extract, and thus the antioxidant effect obtained by the antioxidant of the present invention, particularly the antioxidant effect and the antioxidant effect in vivo. Oxidation effect can be enhanced. As the concentration treatment, a soluble component recovery process and / or an insoluble component recovery process utilizing solubility in water and / or an organic solvent, a liquid-liquid distribution process with a water-hydrophobic organic solvent, a recrystallization process, and a reprecipitation process , A process of collecting a precipitate generated by cooling, etc., can be performed. Examples of fractionation and purification include recrystallization, reprecipitation, purification by normal phase and / or reverse phase chromatography, decolorization, deodorization, etc. It can be performed. For example, it is possible to improve the active oxygen removal function of the present extract by concentrating by a method utilizing solubility in water, and by performing a treatment such as decolorization and deodorization to make it colorless and odorless. Applications and usability can be improved. In addition, the antioxidant of the present invention containing the extract is also preferable since the antioxidant effect is enhanced, and the use and usability are improved. Since these extracts are easily soluble in water as described above, these antioxidants, antioxidants or in vivo antioxidants are in a liquid state in which a part or all of them is dissolved in water or a water-containing organic solvent. And the effect can be suitably exerted. There is an advantage that the effect is suitably exerted even in a liquid state, and that it is easy to use. The antioxidant, antioxidant or in vivo antioxidant can be added to various products to improve the storage stability of the product, etc., and produce foods and the like having an in vivo antioxidant effect. You can also. Also, by ingesting the antioxidant, antioxidant or in vivo antioxidant alone or in a state of being contained in food or drink, an in vivo antioxidant effect can be obtained. In addition, the present invention can use an extract obtained from olives as an antioxidant, in particular, using an extract obtained from olives as an antioxidant, and using an extract obtained from olives in vivo as an antioxidant. It can be used as an agent. The antioxidant, antioxidant or in-vivo antioxidant of the present invention may be used directly for internal use or the like to obtain its effect, or may be blended as a raw material for various products. For example, it can be used as an antioxidant to improve the storage stability of foods and drinks, skin external preparations, pharmaceuticals, feeds, etc., and when added to foods and drinks, contains natural ingredients with excellent storage stability It is possible to produce foods and drinks that are effective for lifestyle-related diseases. The blending amount is not particularly limited, and a suitable amount of extract can be blended according to the food or drink. Here, the antioxidant and the like of the present invention are preferable because they are derived from a plant called olive, which is excellent in safety to the human body, and can be stably supplied because it is a general plant.

The olive plant (Olea europaea L.) used as a raw material of the present invention can be used irrespective of the place of origin such as domestic and European origins, edible or oiled. The extract used in the antioxidant of the present invention can be obtained mainly from fruits or seeds of an olive plant, which is a natural plant, and can be obtained from its peel, stem and bud.
Moreover, it can also be suitably obtained from these dried, pulverized and degreased products. Further, when the fruits of the above olive plants and their defatted products are added with water, etc., when humidification treatment such as steaming is performed, the fruits of these olive plants and their defatted products swell appropriately. The extraction efficiency is improved, which is preferable.

[0016] In particular, an extract is obtained from a defatted olive plant in a very high yield, and it is not necessary to remove oil from the obtained extract. Further, the lipid component contained in the olive plant or the defatted product is a hydrocarbon such as pentane, hexane, heptane, a lower fatty acid alkyl ester such as ethyl acetate, one of known water-insoluble organic solvents such as diethyl ether or A defatted material obtained by extracting and removing with two or more kinds and further repeating this washing treatment as necessary can be used.

The extract used for the antioxidant of the present invention can be obtained by extracting an olive plant with water and / or an organic solvent.

The organic solvent used for obtaining the extract used as the antioxidant of the present invention from olive plants may be either a hydrophilic organic solvent or a hydrophobic organic solvent. Specifically, as the hydrophilic organic solvent, methyl alcohol, ethyl alcohol, glycerin, propylene glycol, 1,
Known organic solvents such as alcohols such as 3-butylene glycol, acetone, tetrahydrofuran, acetonitrile, 1,4-dioxane, pyridine, dimethyl sulfoxide, N, N-dimethylformamide, and acetic acid;
Examples of the hydrophobic organic solvent include known organic solvents such as hexane, cyclohexane, carbon tetrachloride, chloroform, dichloromethane, 1,2-dichloroethane, diethyl ether, ethyl acetate, benzene, and toluene. Also,
These organic solvents can be used alone or in combination of two or more.

Industrially, for example, permeability into plant tissue,
From the viewpoint of extraction efficiency and the like, it is preferable to use water and / or a hydrophilic organic solvent, and it is preferable to use a water-containing hydrophilic organic solvent. Specifically, methyl alcohol, ethyl alcohol, glycerin, propylene glycol,
Examples include alcohols such as 1,3-butylene glycol, organic solvents such as acetone, tetrahydrofuran, and acetonitrile, and hydrated solvents thereof. With one or more selected from these, an extract used in the antioxidant of the present invention can be obtained from olive plants.

Taking advantage of the characteristics of the solubility of the extracted components, water and / or a hydrophilic organic solvent, preferably a water-containing hydrophilic organic solvent, more preferably a water content Extraction using a water-containing hydrophilic organic solvent having a high water content is preferred.

The extraction conditions are not particularly limited. For example, the temperature is 5 ° C. to 95 ° C., preferably 10 ° C. to 90 ° C.
More preferably, it can be extracted at a temperature of 15 ° C to 85 ° C at room temperature. The higher the temperature, the higher the extraction efficiency tends to be. The pressure may be normal pressure, increased pressure, or reduced pressure by suction or the like. Further, in order to improve the extraction efficiency, the extraction can be performed by shaking extraction or an extractor equipped with a stirrer or the like. Although the extraction time depends on other extraction conditions, it is several minutes to several hours, and the longer the time, the more sufficient extraction is performed. However, the extraction time may be appropriately determined according to production conditions such as production equipment and yield. The solvent used for the extraction may be used in an amount of 1 to 100 times (“mass / mass”; the same applies hereinafter), preferably 1 to 20 times the amount of the raw material.

In consideration of the yield of the obtained extract and the subsequent recovery method, it is preferable to use water and / or a hydrophilic organic solvent, and it is preferable to use a water-containing hydrophilic organic solvent. In the case of performing an extraction treatment with a water-containing hydrophilic organic solvent, the content of the hydrophilic organic solvent is 10% by mass to 9% by mass.
Extraction with a water-containing hydrophilic organic solvent of 5% by mass is preferable, and the content of the hydrophilic organic solvent is preferably 20% by mass to 9% by mass.
It is preferable to use 5% by mass of a water-containing hydrophilic organic solvent, and most preferably, the content of the hydrophilic organic solvent is 30% by mass or less.
It is preferably a water-containing hydrophilic organic solvent adjusted to 95% by mass.

When the strength of the active oxygen removing function of the obtained extract is also taken into consideration, the content of the hydrophilic organic solvent in the extraction with a water-containing hydrophilic organic solvent is 10% by mass.
It is preferable to extract with the above-mentioned hydrated hydrophilic organic solvent, more preferably to use a hydrated hydrophilic organic solvent having a hydrophilic organic solvent content of 40% by mass or more, and most preferably to use a hydrophilic organic solvent content of 40% by mass or more. It is preferably a water-containing hydrophilic organic solvent adjusted to be from 80% by mass to 80% by mass.

In view of safety in use to foods and drinks, and use in humans including application to skin, etc.,
It is preferred to extract with either water and / or alcohol.

From the viewpoint of the yield of the obtained extract and subsequent recovery, it is preferable to use water and / or alcohol, and it is preferable to use aqueous alcohol. In the case of the extraction treatment with hydroalcohol, it is preferable to extract with hydroalcohol having an alcohol content of 10% by mass to 95% by mass, and further use hydrous alcohol having an alcohol content of 20% by mass to 95% by mass. It is preferable that the alcohol content is 30% by mass to 95% by mass.

When the strength of the active oxygen removing function of the obtained extract is also taken into consideration, it is preferable to extract with a hydrous alcohol having an alcohol content of 10% by mass or more. Further, it is preferable to use a hydrated alcohol having an alcohol content of 40% by mass or more, and most preferably a hydrated alcohol having an alcohol content adjusted to 40% by mass to 80% by mass.

Here, the alcohol used in the present invention is:
Primary alcohols such as methyl alcohol, ethyl alcohol, 1-propanol and 1-butanol; secondary alcohols such as 2-propanol and 2-butanol; tertiary alcohols such as 2-methyl-2-propanol; and ethylene glycol and propylene glycol And known solvents such as liquid polyhydric alcohols such as 1,3-butylene glycol. These solvents can be used alone or in combination of two or more.

Industrially, it is particularly preferable to use a lower alcohol in view of production cost and handling such as removal of a solvent by distillation. here,
The lower alcohol includes known alcohols having 1 to 4 carbon atoms, for example, the above-mentioned primary, secondary, tertiary or liquid polyhydric alcohols.
More than one species can be used in combination.

Other products generated in the process of producing olive oil, for example, pressed residue, extracted residue, pressed oil, extracted oil, degummed oil residue, deoxidized oil residue, dark oil, waste decolorizing agent, deodorized scum, pressed oil Juice, wastewater, and waste filter media are also included in olive plants, from which the present extract having an active oxygen removing function can be obtained. It is a preferable method for producing the extract from the viewpoint of effective utilization of olives. Here, an extract having an active oxygen removing function can be obtained from wastewater generated in the olive oil pressing step. In particular, when olive oil is squeezed, squeezing the olive fruits yields what is called a juice containing oil and water, from which water is removed and olive oil is obtained. An extract is obtained.

As described above, an extract having an active oxygen removing function can be produced by extraction using water and / or an organic solvent. In addition, components such as water solubility of the present extract have a strong active oxygen removing function, and an antioxidant containing the same also has a stronger antioxidant effect.

By removing the solvent and water from the thus obtained extract, a dry extract used as the antioxidant of the present invention can be obtained. The removal of the solvent and water can be performed by a known method such as vacuum distillation, vacuum / vacuum drying, freeze drying, spray drying and the like. Needless to say, the solvent and water may be contained, or the aqueous solution may be obtained by removing only the solvent. In addition, the solvent,
It is also possible to prepare an aqueous solution in which the concentration of the extract is adjusted by adjusting the degree of water removal, that is, the strength of the active oxygen removing function is adjusted. Further, even with the poorly water-soluble components contained therein, an aqueous solution from which they have been removed may be used.

Further, according to the production method of the present invention, an extract having an active oxygen removing function can be obtained in a higher yield per unit of raw material than in the case of using sesame, for example, in a higher yield than other natural raw materials. It can be obtained in 10 to 10 times the yield.

The extract from the defatted product is preferable because it does not contain oil-soluble components such as triglyceride, sterol, and tocopherol, and it is not necessary to remove and purify these components.

Also, products generated in the process of producing olive oil, such as pressed residue, extracted residue, pressed oil, extracted oil, degummed oil residue, deoxidized oil residue, dark oil, waste decolorizing agent, deodorized scum, and oil pressed Since an extract having an active oxygen removing function can be obtained from juice, wastewater, and waste filter material, it is an extremely effective method for effectively using olives, and also an excellent method from the viewpoint of production cost.

These extracts may be used as they are, but, if necessary, may be used after further concentration and / or fractionation / purification.

The concentration treatment is not particularly limited. For example, the concentration treatment may be a treatment for recovering soluble and / or insoluble components utilizing solubility in water and / or an organic solvent, or a treatment for recovering insoluble components using a water-hydrophobic organic solvent. Liquid-liquid distribution processing,
The concentration treatment can be suitably performed by one or more treatments selected from a recrystallization treatment, a reprecipitation treatment, and a treatment for collecting a precipitate generated by cooling.

For example, by using a concentration method utilizing solubility in water, a component that is easily soluble in water, that is, a component that is soluble in water, and a component that is hardly soluble in water and / or a component that is not soluble in water, that is, It can be separated into components having poor water solubility. Here, it has been found that a concentrate of a component such as water-soluble has a higher active oxygen removing function than a component having poor water-solubility or the like.

As one of the concentration methods, a concentrate can be easily obtained in the process of removing the solvent from the extract obtained from olive plants. That is, in the aqueous solution from which only the solvent has been removed, a water-soluble component and a water-insoluble component are separated by filtration, centrifugation, decantation, and the like, and the water-soluble component is dehydrated and dried. And a water-soluble concentrate having the following formula: As described above, since a concentrate can be obtained by a very simple method, it can be said that this is a very excellent concentration method in terms of efficiency.

Further, by adding and stirring water to the extract extracted and dried from the olive plant, a component that is hardly soluble in water and / or a component that does not dissolve in water, that is, a component having poor water solubility and the like, is mixed with water. Separation into readily soluble components allows for significant concentration. Concentrates such as water-soluble substances contained in extracts from olive plants have a significantly superior active oxygen removing function, even when compared with the whole extract or components such as poor water solubility in the extracts. confirmed.

As described above, the concentrate can be easily obtained by adding an extract from an olive plant to water and stirring the resulting mixture, and then separating a soluble component from an unnecessary component. In addition, by adjusting the amount and temperature of water, it is possible to adjust the amount of the obtained components such as water solubility and the strength of the active oxygen removing function.

The extract can be concentrated, if necessary, by liquid-liquid distribution using a common solvent combination. Solvent combinations are generally difficult to define, for example,
Examples of water-hydrophobic organic solvents include hydrophobic organic solvents such as hexane, carbon tetrachloride, chloroform, dichloromethane, 1,2-dichloroethane, diethyl ether, ethyl acetate, n-butanol, benzene, and toluene. Known organic solvents can be used. In this liquid-liquid distribution, by removing water in the aqueous phase, the present extract having an active oxygen removing function can be obtained.

Further, the present extract and the extract obtained by concentrating the extract can be subjected to fractionation and purification. Thereby, it is possible to concentrate more than the above concentration. Advantages of the fractionation / purification treatment include not only that the active oxygen removing function can be greatly improved, but also that impurities can be removed. That is, the fraction
The purification treatment is preferable because it has the advantage that the antioxidant of the present invention can be suitably blended without adding an extra color.

The method of fractionation / purification is not particularly limited. For example, one or two selected from purification by normal phase and / or reverse phase chromatography, recrystallization, reprecipitation, decolorization, and deodorization By the above processing, fractionation and purification can be suitably performed. In particular, among the chromatography methods, the method using liquid chromatography is preferable because the extract and concentrate used for the antioxidant of the present invention can be fractionated and purified in good yield without decomposing. Specific examples of liquid chromatography include normal-phase liquid chromatography, reverse-phase liquid chromatography, thin-layer chromatography, paper chromatography, and high-performance liquid chromatography (HPLC). Can be used in any fractionation and purification treatment. In particular, normal phase liquid chromatography, reverse phase liquid chromatography, and high performance liquid chromatography (HPLC) are preferable in consideration of the separation ability, the throughput, the number of steps, and the like.

Here, normal phase liquid chromatography refers to the following method, for example. That is, for example, a column was prepared using silica gel as a stationary phase, a hexane-ethyl acetate mixed solution, a chloroform-methanol mixed solution, or the like as a mobile phase, and an extract from an olive plant or a concentrate thereof was loaded at a load rate of 0.1 to 5% (wt. (Mass) / v (volume)), and a predetermined fraction is eluted by a continuous elution method using a single mobile phase or a stepwise elution method in which the solvent polarity is gradually increased. What is reverse phase liquid chromatography?
For example, it refers to the following method. That is, for example, a column is prepared using silica (ODS) to which octadecylsilane is bonded as a stationary phase, a water-methanol mixture, a water-acetonitrile mixture, a water-acetone mixture, or the like as a mobile phase. Load the concentrate at 0.
This is a method in which a predetermined fraction is applied at 1 to 5% (wt (mass) / v (volume)) and a predetermined fraction is eluted by a continuous elution method using a single solvent or a stepwise elution method in which the polarity of the solvent is gradually reduced. What is high performance liquid chromatography (HPLC)?
In principle, it is the same as the normal phase liquid chromatography or the reverse phase liquid chromatography described above, and is used for fractionation and purification with higher speed and higher resolution. By combining one or more of the above methods,
It is preferable because it can be very concentrated and can be obtained with impurities removed. Furthermore, by combining one or more of the above methods, the concentration for obtaining the active oxygen removing function of the present extract can be adjusted,
The strength, characteristics, etc. of the function can be designed as needed.

The concentration treatment may be repeated concentration treatment, or different concentration treatments may be combined. Similarly, the fractionation / purification treatment may be repeated fractionation / purification treatment, and different fractionation / purification treatments may be performed.
Purification treatments may be combined. Furthermore, fractionation / purification treatment may be performed after concentration treatment, fractionation / purification treatment may be performed after fractionation / purification treatment, and fractionation / purification treatment may be performed after concentration treatment, followed by further concentration. It can also be processed. Of course, a combination other than the above-mentioned combination may be used.

When an extract obtained from an olive plant is subjected to a concentration treatment and a further fractionation / purification treatment to be decolorized and / or deodorized, unnecessary components are removed, and the colorless to pale color is obtained. And / or odorless to almost odorless, so that use is not restricted by color or fragrance, and a wide range of applications can be ensured. Examples of the decolorization method include activated carbon treatment and clay treatment. Examples of the deodorization method include activated carbon treatment and clay treatment, and further include supercritical extraction and steam distillation.

Also, products generated in the process of producing olive oil, such as pressed residue, extracted residue, pressed oil, extracted oil, degummed oil residue, deoxidized oil residue, dark oil, waste decolorizing agent, deodorized scum, and oil pressed An extract having a function of removing active oxygen can be obtained from juice, wastewater, and waste filter material, but can be concentrated and fractionated and purified in the same manner.

The combination of concentration and / or fractionation / purification is not particularly limited, but the extract used for the antioxidant of the present invention is easily soluble in water. In the concentration, fractionation and purification treatments, the active oxygen removal function of the present extract can be improved by concentrating water-soluble components. The combination of the extraction treatment, the concentration treatment and / or the purification treatment is not particularly limited. For example, after an olive plant is subjected to an extraction treatment with water and / or a hydrophilic organic solvent, the obtained extract is treated with a hydrophilic organic solvent. Part or all is removed, and the water-soluble matter is recovered to concentrate. Although it can be collected by filtration, centrifugation, or the like, in order to improve the collection efficiency, it is possible to add water to the aqueous solution and perform agitation or the like as needed. In addition, the extract in the dry state obtained by removing the water and / or the hydrophilic organic solvent from the extract obtained from the olive plant is subjected to treatment such as addition of water and stirring in the same manner as described above, and the water is removed by filtration or the like. The concentration treatment can be performed by recovering the dissolved component. These concentrates can be fractionated and purified by normal phase and / or reverse phase chromatography and / or recrystallization.

The obtained extract and the extract obtained by subjecting the extract to a concentration treatment and / or a purification treatment can be used alone or in combination of two or more kinds. Thereby, the characteristics of the strength of the active oxygen removing function can be designed, and it can be used as an extract having a more suitable active oxygen removing function. In addition, other antioxidants can be blended and used, which enables detailed design such as the strength of the effect, and also has a significant functional enhancement due to the synergistic effect with other functional substances. Can be expected.

The component having an active oxygen removing function of the extract used as the antioxidant of the present invention can be suitably blended particularly with an aqueous product. This extract can be suitably used for water-based products because the components that are easily dissolved in water have an antioxidant effect. Many common antioxidants are oil-soluble (water-insoluble) such as, for example, tocopherols derived from vegetable oils, and are often restricted in actual use. Since the component has water solubility (contains a water soluble component), its application range is very wide, and the antioxidant using this extract can be said to be a highly versatile antioxidant. The antioxidant of the present invention can be suitably used in a wide range of foods and drinks such as an aqueous system or an emulsified system, and can be uniformly dissolved or dispersed and contained.

In addition, the use of the antioxidant of the present invention in an oil system is particularly attributable to the fact that components which are difficult to dissolve in oil and components which do not dissolve in oil are contained in a relatively large amount, especially in the mere extraction stage. Therefore, it is preferable to use an oil or fat or the like as an emulsified product by blending an extract that has been subjected to purification, such as removal of insoluble components, or by blending water and an emulsifier.

As described above, the extract used for the antioxidant of the present invention has a function of removing active oxygen. The active oxygen removing function refers to a function of suppressing, capturing, eliminating, disproportioning, decomposing, etc., the generation of active oxygen species in a living body or in foods, pharmaceuticals, agricultural chemicals and the like. More specifically, for example, suppression of active oxygen generation by chelation and inactivation of metal ions that cause generation of active oxygen species, elimination or decomposition of generated active oxygen species, disproportionation by enzymes, etc., radical Suppression and blocking of the radical chain reaction by trapping or stabilization, and the like. Here, the active oxygen species mainly include superoxide, hydroxy radical, perhydroxy radical,
The term refers to hydrogen peroxide, singlet oxygen, and the like, and further includes peroxides such as lipids, proteins, carbohydrates, and nucleic acids, and free radicals derived therefrom. These reactive oxygen species strongly oxidize various components such as lipids, proteins, carbohydrates, and nucleic acids in foods and living bodies, and convert or decompose them into components different from the original components. Therefore, this function is useful as a protective agent against oxidative deterioration of components contained in the in vivo or external preparation for skin and the like. The extract has high superoxide scavenging activity and hydroxy radical scavenging activity, among others.

The superoxide scavenging activity is an activity that disproportionates and invalidates superoxide generated by one-electron reduction of oxygen molecules. Superoxide is produced, for example, in leukocytes, mitochondria, and the like in a living body, and is inevitable in the life activity utilizing oxygen. In addition, superoxide has relatively low reactivity and reacts only with limited substances such as iron and nitric oxide, but leads to generation of other active oxygen species such as a source of hydrogen peroxide, It is an important reactive oxygen species that has the effect of causing oxidative damage to biological components and should be eliminated immediately after its production. Superoxide dismutase (SOD) has a superoxide scavenging effect and is an in vivo enzyme possessed even by prokaryotes. To protect biological components more aggressively, a substance having superoxide scavenging activity must be used. It is desirable to apply to living bodies. However, for example, this SOD is an enzyme protein and has poor stability, and when administered orally, most of it is excreted, so that its effective power is extremely low. In other words, this extract can enjoy its superoxide elimination activity stably when ingested, so that it protects the biological components more effectively to maintain a healthy body or to make the skin beautiful It greatly contributes to the above.

Although the present extract is at the extract level, it has a strong superoxide scavenging activity exceeding BHA which is a synthetic antioxidant having a strong superoxide activity function. In addition to use as an antioxidant, it can also be used as an antioxidant in vivo (antioxidant in vivo).

The hydroxy radical scavenging activity is an activity of capturing and stabilizing a hydroxy radical generated by various factors. Hydroxy radicals, for example, cannot be spared in the life activity utilizing oxygen, and are extremely reactive chemical species among various reactive oxygen species. It is a highly toxic reactive oxygen species. One of the pathways for the generation of this hydroxyl radical is the Fenton reaction in which iron ions present in the living body are involved in hydrogen peroxide and superoxide, but there is an effective removal mechanism for hydroxy radicals in the living body. Therefore, ingestion of a substance having a hydroxy radical scavenging activity is indispensable. Examples of the hydroxy radical removing agent include mannitol, tryptophan, and formic acid. However, many of these require a very high concentration even though they are single products. That is, the antioxidant containing the extract can enjoy its hydroxy radical scavenging activity when ingested, and can effectively protect biological components to maintain a healthy body or to make the skin beautiful. It greatly contributes to the above.

The present extract is capable of eliminating hydroxy radicals (for example, suppressing peroxidation of linoleic acid) with a considerably small amount at the extract level, and can be used as compared with a conventional remover of hydroxy radicals. It can be said that it is very effective. It can be used as a hydroxy radical scavenger and a lipid peroxidant, such as foods and drinks, cosmetics, pharmaceuticals, feeds, etc., which contain antioxidants in vivo (antioxidants in vivo), and components susceptible to oxidation such as lipids. It can also be used as an antioxidant to improve the storage stability of any product.

Furthermore, the above-mentioned SOD has an erasing effect only on superoxide, has no erasing activity on hydroxy radicals, and mannitol similarly cannot erasure superoxide. On the other hand, the present extract shows an elimination effect on both. That is, the present extract has extremely high versatility because it has both active oxygen removing functions of strong superoxide scavenging activity and strong hydroxyl radical scavenging activity. In addition, in terms of effect, it has both an active oxygen removing function of a superoxide scavenging activity and a hydroxy radical scavenging activity, so it is excellent as having an in vivo antioxidant effect. Because of its lipid peroxidation preventing effect, it is excellent as an agent for improving the storage stability of foods and drinks, cosmetics, pharmaceuticals, feeds, and the like in addition to skin external preparations.

When comparing the active oxygen removing function with other natural antioxidants, for example, the extract derived from sesame meal, which is considered to have a relatively strong active oxygen removing function, has a superoxide scavenging activity of 5%.
This extract has a superoxide scavenging activity of 20 to 65 units / (mg / mL).
/ (Mg / mL), 2 to 13 of the extract from sesame meal
It has a very high effect of double.

In this method, an extract having a strong active oxygen removing function can be obtained, and the yield per unit raw material is high. From both of these facts, the active oxygen removal function yield index <Equation 1>, which is obtained by multiplying the strength of the active oxygen removal function by the yield per unit raw material, is significantly higher than the case where other natural raw materials are used. High values are obtained. That is, the total amount of the active oxygen removing function obtained per unit raw material is large. Therefore, by producing and using this extract, not only products with strong active oxygen removal function,
When replaced by other natural antioxidants, etc., the total amount of active oxygen removal function obtained from unit raw materials is large, so that more products can be made, which means that cost reduction The benefits can be expected. Also, by referring to the active oxygen removal function yield index, suitable production conditions can be determined.

[0060]

<Equation 1> Active oxygen removal function yield index = Strength of active oxygen removal function × Yield per unit raw material (%)

As for the superoxide scavenging activity, the extract obtained from the unit raw material has a superoxide scavenging activity yield index (formula 2) of about 100 to 3250. For example, when compared with sesame, the superoxide scavenging activity yield index is 5 to 100, which is a high value of 1 to 650 times that of sesame. That is, the active oxygen removing function can be obtained 1 to 650 times per unit raw material.

[0062]

<Equation 2> Superoxide scavenging activity yield index = superoxide scavenging activity (unit / (mg / m
L)) × Yield per unit raw material (%)

The extract used as the antioxidant of the present invention has a function of removing active oxygen such as superoxide scavenging activity and hydroxy radical scavenging activity, but the antioxidant is used in humans and other foods and drinks, pharmaceuticals, fertilizers and feeds. And various external effects can be obtained by using it for skin and external preparations. As mentioned before, for example, an antioxidant effect, especially an excellent antioxidant effect such as an effect of improving the storage stability of the product,
Excellent in vivo antioxidant effects such as various disease prevention effects and anti-aging effects can be obtained.

The antioxidant effect refers to suppressing or preventing various components such as lipids, proteins, carbohydrates, and nucleic acids in a food or a living body from being oxidized and converted or decomposed into components different from the original components. The effect is. Therefore, this effect is useful in terms of protection against oxidative degradation of components in vivo or contained in foods, pharmaceuticals, agricultural chemicals, etc., and in addition to the food industry, especially fishery products, health foods, nutritional foods,
It is expected to be used practically in the fields of pharmaceuticals / pesticides and cosmetics. Specifically, it is possible to obtain an antioxidant effect such as an effect of improving the preservability of products such as foods and drinks, pharmaceuticals, fertilizers, feeds and external preparations for skin, an antioxidant effect in vivo such as an effect of preventing various diseases and an antiaging effect. it can.

The antioxidant effects include foods and drinks, skin external preparations,
It has an effect of preventing oxidative deterioration of easily oxidizable components contained in pharmaceuticals, feeds, and the like, especially lipids containing unsaturated fatty acids. Oxidative degradation of components contained in foods and drinks, skin external preparations, pharmaceuticals, feeds, and the like is a major cause of storage stability problems such as deterioration of quality, nutritional and functional deterioration. Therefore, if the oxidation of various components can be suppressed,
It is possible to stabilize and maintain the quality of skin external preparations, pharmaceuticals, feeds and the like. In this regard, the extract is
Since it has an active oxygen removing function, it can be expected to minimize the oxidation of various components, which is very preferable. That is, the antioxidant containing the extract prevents the oxidation of components contained in foods and drinks, skin external preparations, pharmaceuticals, feeds, and the like, thereby improving storage stability, improving quality, and improving nutritional properties and functions. It greatly contributes to maintaining the properties.

The antioxidant of the present invention has a clear antioxidant effect when foods and drinks and cosmetics containing the antioxidant are actually prepared, as compared with the equivalent products not containing the antioxidant. Have. Therefore, the antioxidant of the present invention can be used as an antioxidant. That is, for example, after preparing a cookie and storing it in a dark place at 60 ° C. for 18 days, oil is extracted from the stored cookie in accordance with a conventional method,
When the OV is measured and compared, it can be seen that the antioxidant of the present invention clearly prevents the oxidation of mainly fats and oils of cookies and has a high antioxidant effect. At the same time, the antioxidant effect of the antioxidant of the present invention, when actually making foods and drinks and cosmetics containing the antioxidant, compared to the equivalent product without the antioxidant, clearly It is clear from the improved storage stability. That is, for example, a cookie made and stored at 60 ° C. in a dark place for 18 days before tasting showed that the cookie not containing the antioxidant had a considerable oxidatively degraded odor, The cookie containing the antioxidant of the present invention clearly retains a good flavor, indicating that the antioxidant effect of the antioxidant has improved storage stability. By using the antioxidant of the present invention as an antioxidant in various products, the effect of improving the storage stability of the product can be obtained.

The in vivo antioxidant effect has an effect of protecting biological components important in life by removing active oxygen species that cannot be avoided in the living body. In general, reactive oxygen species generated in a living body strongly oxidize vital biological components such as lipids, proteins, carbohydrates, and nucleic acids, particularly lipids containing unsaturated fatty acids, and become active components. Is converted or decomposed into different components. Furthermore, it is known that the accumulation of such phenomena in the body causes various diseases and aging phenomena, but the application of those having an antioxidant effect in vivo has been applied to these diseases and aging. It has also become clear that it can lead to prevention and improvement. Therefore, the antioxidant of the present invention protects biological components by the in vivo antioxidant effect obtained by the active oxygen removing function of the present extract, and thus various diseases caused by active oxygen species and the like. It has various disease prevention effects and / or anti-aging effects that can prevent and improve the aging phenomenon.

The in vivo antioxidant effect of the antioxidant of the present invention is as follows:
For example, it can be examined by animal tests. That is, the difference between the in vivo antioxidant power of a rat fed a diet containing the antioxidant and the feed of a normal diet composition using a rat. Is to be considered. This result shows a similar tendency for humans. Specifically, the in vivo antioxidant power may be, for example, the antioxidant power of the rat liver itself. In this test, when the feed containing the antioxidant was given, the antioxidant power in the living body was significantly improved as compared with the case where the feed having the usual feed composition was given. That is, it is clear that the antioxidant of the present invention clearly has an antioxidant effect in vivo. Similarly, when the dose of the antioxidant is increased or decreased, the in vivo antioxidant power fluctuates in a dose-dependent manner. That is, this shows that the dose of the antioxidant and the antioxidant effect in vivo are almost proportional, and it can be said that the antioxidant effect in vivo of the antioxidant is dependent on the dose. . This means that when the antioxidant of the present invention is developed for various uses, it is possible to obtain an antioxidant effect in a living body according to the amount of the antioxidant used. It is very preferable because it has the merit that it can be determined.

The in vivo antioxidant effect of the antioxidant of the present invention can be evaluated by a test ingested by humans. For example, the present extract may be used as it is, or a tablet, powder, capsule or the like containing the extract may be used in various other dosage forms. The purpose of the present invention is to evaluate what difference is produced in comparison with the case where a substance is not orally ingested. In this case, the in vivo antioxidant activity is, specifically, for example, a test subject's blood is collected at regular intervals (for example, every 10 days) during the test period, and serum is prepared by centrifugation or the like. What is necessary is just to measure the amount of lipid peroxide (PCOOH). In this test, when a person orally ingests the antioxidant of the present invention, the amount of lipid peroxide decreases day by day from the start of the test, and the amount of lipid peroxide production is significantly suppressed after 30 days. On the other hand, when this extract is orally ingested without blending, since such an effect of suppressing lipid peroxide production is not seen at all, when ingesting the antioxidant of the present invention, It can be seen that the in vivo antioxidant power is significantly improved. That is, it is understood that the antioxidant of the present invention clearly has an in vivo antioxidant effect on the human body. Furthermore, when used as the antioxidant of the present invention, particularly in vivo, it exerts various disease preventing effects and / or antiaging effects.

Olive, which is a raw material of the present extract, is a plant raw material that can be stably obtained. Since it is an extract obtained from olives, which are naturally derived and widely used as food and food, it can be said that the extract is highly safe for the human body.

According to the present invention, an antioxidant containing an olive extract having an active oxygen removing function can be obtained. Furthermore, since the extract having an enhanced active oxygen removing function can be obtained by concentrating, fractionating and purifying the extract, the extract has the function of the desired strength by adjusting the concentration conditions and the like. The extract can be obtained, and an antioxidant having an antioxidant effect of the required strength can be obtained by containing the extract. In addition, the antioxidant can be used for various uses such as foods and drinks and external preparations for skin. In addition, it can be easily obtained from olive plants,
In addition, since it can be suitably obtained from the normally discarded product generated during the production process of olive oil, it is preferable from the viewpoint of cost and the effective utilization of resources.

The antioxidant of the present invention can be formulated by a general method. The extract obtained from the olive plant contained as an active ingredient may be a crude extract or a concentrated and / or fractionated / purified extract, and particularly an extract obtained by concentrating and / or fractionating / purifying a water-soluble component. It is preferable to use With respect to the extract obtained from olive plants, it is preferable to use an extract obtained by concentrating the water-soluble component since the fraction of the water-soluble component has a strong active oxygen removing function.

The form of the antioxidant of the present invention includes
Powders, capsules (hard capsules, soft capsules, etc.), granules (coated, pills, troches, liquids, or pharmaceutically acceptable sustained-release preparations thereof) and the like. .

These preparations may be used together with pharmacologically acceptable bases, carriers, excipients, disintegrants, lubricants, coloring agents, etc. according to known pharmaceutical preparation methods.

The carriers and excipients used in these preparations include, for example, lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate,
Calcium phosphate, calcium sulfate, crystalline cellulose,
Licorice powder, gentian powder and the like.

The binder used in these preparations includes, for example, starch, tragacanth, gelatin, syrup,
Examples include polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, carboxymethyl cellulose, and the like.

The disintegrants used in these preparations include, for example, starch, agar, gelatin powder, sodium carboxymethylcellulose, calcium carboxymethylcellulose, crystalline cellulose, calcium carbonate, sodium hydrogencarbonate, sodium alginate and the like.

Examples of the lubricant used in these preparations include magnesium stearate, talc, hydrogenated vegetable oil, macrogol and the like.

As the coloring agents used in these preparations, those permitted to be added to pharmaceuticals can be used.

When preparing injections, if necessary, a pH adjuster, a buffer, a stabilizer, a solubilizing agent and the like are added, and each injection is prepared by a conventional method.

When preparing tablets and granules, sucrose, gelatin, hydroxypropylcellulose, purified shellac, gelatin, glycerin, sorbitol, ethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinylpyrrolidone, phthalic acid It may be coated with cellulose acetate, hydroxypropyl methylcellulose phthalate, methyl methacrylate, methacrylic acid polymer, or the like, or may be coated with two or more layers. Further, capsules made of a substance such as ethyl cellulose or gelatin may be used.

Examples of the form of the external preparation include solid, semi-solid, semi-solid or liquid preparations for transdermal administration or transmucosal administration such as intraoral or intranasal administration. Examples of the liquid preparation include pharmaceutically acceptable emulsions or emulsions such as lotions, tinctures for external use, and liquid preparations for transmucosal administration. This preparation contains, for example, ethanol, oils, emulsifiers and the like as commonly used diluents. Examples of semisolid preparations include ointments such as oily ointments and hydrophilic ointments. This preparation contains, for example, water, petrolatum, polyethylene glycol, oil, surfactant and the like as a commonly used base or carrier.
Examples of semi-solid or solid preparations include patches for transdermal administration such as plasters (rubber plaster, plaster, etc.), films, tapes, and cataplasms or for transmucosal (intraoral, nasal) administration. No. This formulation is usually used as a base or carrier, for example, natural rubber,
Rubber-based polymers such as butadiene rubber, synthetic rubber such as SBR and SIS, mud-forming agents such as gelatin, kaolin, and zinc oxide; hydrophilic polymers such as sodium carboxymethylcellulose and sodium polyacrylate; acrylic resins; liquid paraffin And tackifiers, water, other oils, and surfactants. These preparations may further use adjuvants such as stabilizers, solubilizers, and transdermal absorption enhancers, or additives such as fragrances and preservatives.

The antioxidant of the present invention contains the present extract as an active ingredient. The active ingredient indicates that the extract is contained to such an extent that the intended function is exhibited. Although the content is not particularly limited, it can be appropriately adjusted depending on the degree of the intended function, the mode of use, the amount of use, the degree of concentration, fractionation and purification of the extract, for example, 0.01 to 100% by mass. , Preferably 0.1 to 99.9% by mass, more preferably 0.5 to 99.5% by mass. This extract can be obtained by extracting the product obtained in the olive plant and / or olive oil production process with water and / or an organic solvent, and further subjecting the extract to a concentration treatment and / or a fractionation / purification treatment. This makes it possible to obtain an active oxygen remover with enhanced effects. The active oxygen remover can be used for humans and other foods and drinks, pharmaceuticals, fertilizers, feeds and external preparations for skin. In addition, it may be taken orally, etc.
It can also be applied to the skin and the like.

The antioxidant of the present invention can be incorporated into oral and parenteral products according to ordinary methods, and can be used in various forms such as food and drink, external preparations for skin, pharmaceuticals, quasi-drugs, feed, feed, agricultural chemicals and the like. It can be used in various fields. For example, when incorporated in foods and drinks, foods and drinks having in vivo antioxidant and antioxidant effects can be provided. The food and drink can be expected to have an antioxidant effect such as suppression of flavor deterioration and discoloration, and an effect of preventing lifestyle-related diseases. From the effects of preventing lifestyle-related diseases, it can be expected to be used as health foods, nutritional foods and the like. Furthermore, by adjusting conditions such as concentration of the extract, a food or drink containing the extract can be provided to provide a functional food or drink that exerts a further effect by continuously ingesting the food or drink. In addition, it can be used for livestock, fish feed and feed, especially fish feed that requires a lot of water and is susceptible to oxidative deterioration when left unattended. However, it is possible to provide a feed or the like in which these are improved. In addition, there are some fish for appreciating or farming that are difficult to breed, but feeds using the antioxidant of the present invention have effects such as in vivo antioxidant effects. It is possible to provide a feed or the like that contributes to the improvement of these breeding and survival rates.

The antioxidant of the present invention may be used in humans and other foods and drinks,
Used in pharmaceuticals, fertilizers, feeds and skin external preparations, it has excellent antioxidant effects, especially excellent antioxidant effects such as improving storage stability of products, and various disease prevention effects and antiaging effects. An in vivo antioxidant effect can be obtained.
Furthermore, since it can be easily obtained from olive plants, and can be suitably obtained from normally discarded products generated during the process of producing olive oil,
It is preferable from the viewpoint of cost and the effective utilization of resources.

[0086]

EXAMPLES Next, the present invention will be further described with reference to examples, but the present invention is not limited to these examples.

Various extracts were produced in Examples 1 to 17 and Comparative Example 1. The yield is shown in the following <Equation 3>.
I asked for it.

[0088]

<Equation 3> Yield of the extract = mass of the extract (Note 1) 質量 mass of the raw material before extraction (Note 2) Note 1: After drying by freeze-drying, the mass (g) Measurement Note 2: The extract, fruit, lees, immediately before extraction with a solvent, etc.
Measure the mass (g) of seed

Example 1 Domestic olives (Olea europaea L.)
1 kg of dried fruit (including seeds) was crushed, and 3 L of hexane was added to extract for 3 hours. After the seeds were removed from the defatted fruit obtained by repeating this procedure four times, the seeds were removed, pulverized, and extracted again with 5 times the amount of hexane for 3 hours to obtain 229 g of defatted cake from which oil was completely removed. To this defatted lees, a 10-fold amount of an aqueous ethanol solution containing 60% by mass of ethanol was added, and the mixture was extracted at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 112.7 g of an extract. The obtained extract was evaluated for superoxide scavenging activity and hydroxy radical scavenging activity. The results are shown in Tables 1 and 2.

Example 2 To a defatted cake obtained in the same manner as in Example 1 was added a 10-fold amount of a water-containing aqueous ethanol solution having an ethanol content of 80% by mass, followed by extraction at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain an extract of 79.7.
g was obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 3 To a defatted cake obtained in the same manner as in Example 1 was added a 10-fold amount of a water-containing ethanol aqueous solution having an ethanol content of 70% by mass, followed by extraction at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, the filtrate was concentrated to dryness and the extract was 97.8.
g was obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 4 To a defatted cake obtained in the same manner as in Example 1 was added a 10-fold amount of an aqueous ethanol solution containing 50% by mass of ethanol, followed by extraction at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain an extract.
1 g was obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 5 To a defatted lees obtained in the same manner as in Example 1, a 10-fold aqueous ethanol solution containing 40% by mass of ethanol was added, and extraction was carried out at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, the filtrate was concentrated to dryness to extract 112.1.
9 g were obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 6 To a defatted lees obtained in the same manner as in Example 1, a 10-fold amount of an aqueous ethanol solution containing 20% by mass of ethanol was added, and the mixture was extracted at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, the filtrate was concentrated to dryness to extract 110.
6 g were obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 7 A 10-fold amount of absolute ethanol was added to the defatted lees obtained in the same manner as in Example 1, and the mixture was similarly extracted at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 13.5 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 8 A 10-fold amount of water was added to the defatted cake obtained in the same manner as in Example 1, and the mixture was extracted at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, the filtrate was concentrated to dryness to extract 11
8.2 g were obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 9 To a defatted cake obtained in the same manner as in Example 1 was added a 10-fold amount of hydrated acetone having an acetone content of 60% by mass, and the mixture was extracted at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 51.5 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 10 To a defatted cake obtained in the same manner as in Example 1 was added 10 times the amount of aqueous THF having a THF content of 60% by mass, followed by extraction at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 54.3 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 11 To the defatted lees obtained in the same manner as in Example 1, a 10-fold amount of water-containing AN having an AN content of 60% by mass was added, and the mixture was similarly extracted at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 49.6 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 12 1 kg of a dried olive fruit from which seeds were removed was crushed, and a 20-fold aqueous ethanol solution containing 60% by mass of ethanol was added to the crushed fruit, and the mixture was similarly vigorously stirred at room temperature. Extracted for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness, and 238.5 g of the extract was added with 1. 1 g of hexane.
After 5 L and 1.5 L of water were added and stirred well, the separated aqueous phase was concentrated to dryness to obtain 126.4 g of a partitioned extract.
Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 13 500 g of olive seeds were crushed, and a 10-fold amount of an aqueous ethanol solution containing 60% by mass of ethanol was added to the crushed seeds, followed by extraction at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 42.0 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 14 Olives from Italy (Olea europaea)
L. ) Was squeezed, 3 L of hexane was added to 1 kg of the obtained pressing residue, and the mixture was extracted for 3 hours. The defatted residue (884 g) obtained by repeating this process four times was appropriately removed with a sieve to remove seeds and contaminants to obtain 196 g of defatted cake. To this defatted lees, a 10-fold amount of an aqueous ethanol solution containing 60% by mass of ethanol was added, and the mixture was extracted at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to extract 1
9.2 g were obtained. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 15 To a defatted cake obtained in the same manner as in Example 14 was added a 10-fold aqueous ethanol solution containing 70% by mass of ethanol, followed by extraction at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 17.4 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 16 Olives from Italy (Olea europaea)
L. ) Was squeezed, 3 L of hexane was added to 1 kg of the obtained pressing residue, and the mixture was extracted for 3 hours. About 884 g of the defatted residue obtained by repeating this four times, without removing seeds and the like,
This was pulverized to obtain 873 g of a pulverized degreasing residue. To this pulverized and defatted residue, a 10-fold amount of an aqueous ethanol solution having an ethanol content of 60% by mass was added, and the mixture was extracted at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 39.5 g of an extract. Table 1 shows the results of evaluating the superoxide scavenging activity of the obtained extract.

Example 17 To a pulverized defatted residue obtained in the same manner as in Example 16 was added a 10-fold aqueous ethanol solution containing 60% by mass of ethanol, followed by extraction at room temperature for 3 hours with vigorous stirring. After filtering the whole amount, water was added to the aqueous layer portion of the extract obtained by completely removing ethanol from the obtained filtrate so that the total amount became 830 g for the purpose of increasing the efficiency of water-insoluble content recovery. And stirred vigorously at room temperature for 1 hour. After the whole amount was treated by centrifugation to remove the precipitate, the supernatant collected by decantation was dried to obtain 22.9 g of a concentrate.
Table 1 shows the results of evaluating the superoxide scavenging activity of the extract obtained by concentration.

Comparative Example 1 1 kg of dried sesame seeds was crushed, and 3 L of hexane was added thereto.
Time extracted. This is repeated four times, sesame defatted lees 52
6.4 g were obtained. To this sesame defatted lees, a 10-fold amount of an aqueous ethanol solution having an ethanol content of 60% by mass was added, and the mixture was extracted at room temperature with vigorous stirring for 3 hours. After filtering the whole amount, the filtrate was concentrated to dryness to obtain 58.2 g of an extract. The extract obtained from the sesame seed was evaluated for superoxide scavenging activity and hydroxy radical scavenging activity. The results are shown in Tables 1 and 2.

As Comparative Example 2, a synthetic antioxidant BHA having a synthetic superoxide scavenging activity was used.
Superoxide scavenging activity was evaluated. Table 1 shows the results
Shown in

The evaluation methods and results of the superoxide elimination activity and the hydroxy radical elimination activity regarding the active oxygen removing function are described below.

<Evaluation method of superoxide elimination activity>
Evaluation of superoxide scavenging activity was conducted by Naoshi Kaneda and Nobuo Ueda, "Lipid peroxide experimental method", pp. 136-154,
According to the method described in 1993 published by Medical and Dental Medicine Co., Ltd., it was measured as follows. 1 mg / mL ED in 1.2 mL of sodium bicarbonate buffer (pH 10.2)
TA solution 50 μL, 1.5 mg / mL bovine serum albumin (BSA) solution 50 μL, 0.6 mg / mL nitro blue tetrazolium (NBT) solution 50 μL, 0.5 m
g / mL xanthine solution 50 μL, and 0.1 mL of “sample solution” in which the olive extract is dissolved at a predetermined concentration
, And left to stand at 25 ° C for 10 minutes. Add 0.1 unit / mL xanthine oxidase (XO
D) 50 μL of the solution was added, stirred, and left at 25 ° C. for 20 minutes. After stopping the enzymatic reaction by adding 50 μL of a 1 mg / mL copper chloride solution, the absorbance value (A) at 560 nm was measured, and a solution obtained by adding the same amount of “buffer” instead of “sample solution” was used as a control solution. Similarly, the absorbance value (B) was measured.
In addition, the absorbance was similarly measured using the copper chloride solution and the “sample solution” or “buffer solution” in which the order of addition was reversed as a reagent blank, and the respective values were defined as a and b.
On the other hand, as a comparative antioxidant, butylhydroxyanisole (BHA) which is a commercially available antioxidant was used. B
For HA, the absorbance value (A) was measured using the above measurement method with the following changes. That is, the sodium carbonate buffer (pH 10.2) was added in an amount of 1.25 mL, and the “BHA solution” dissolved at a predetermined concentration using methanol was 5 mL.
0 μL, and the XOD solution was 0.2 unit.
The one prepared at a concentration of t / mL was used. The absorbance value (B) was measured using a control solution containing the same amount of “methanol” instead of the “BHA solution”. In addition, the absorbance values of the copper chloride solution and the “BHA solution” or “methanol” in which the order of addition was reversed were similarly measured as a reagent blank, and the respective values were defined as a and b. Then, the scavenging activity of superoxide was calculated from the following <Equation 4>. Here, (C) in the formula indicates the sample concentration in the measurement solution system, and the activity of decreasing the absorbance value by 0.1 due to elimination of superoxide is represented by 1 unit (1 u).
nit). The higher the activity value, the stronger the superoxide scavenging action.

[0110]

<Equation 4> Superoxide erasing activity (uni)
t / (mg / mL)) = {(B−b) − (A−a)} /
(C × 0.1)

The superoxide scavenging activity yield index was calculated by the above <Equation 2>.

[0112]

[Table 1]

Table 1 shows that the present extract has a very strong superoxide scavenging activity. In particular, water,
When extracted using anhydrous alcohol or hydrous alcohol, this extract is a natural extract, but has an erasure equivalent to about 2 times as much as BHA, which is a typical synthetic antioxidant. It shows activity. It can be seen that even a mere extract stage has a very strong superoxide scavenging activity. Furthermore, since the extract has a high yield, the superoxide scavenging activity yield index is even higher. As can be seen from this index, it can be seen that more extracts having a strong active oxygen removing function can be obtained from natural raw materials under the same conditions.

<Evaluation method of hydroxy radical scavenging activity> The hydroxyl radical scavenging activity was measured as follows. The reaction system used in this example generates a hydroxyl radical by the Fenton reaction, and malondialdehyde (MD) generated by a reaction between the hydroxyl radical and a fatty acid.
It is based on a method for measuring the thiobarbituric acid-MDA adduct formed when A) is reacted with thiobarbituric acid. That is, a measurement target dissolved at a predetermined concentration is converted to a solution containing a linoleic acid solution (2 mg / mL) and sodium dodecyl sulfate (SDS, 2 mg / mL).
0.46 mL of mM Tris-HCl buffer (pH 7.4)
, And 0.02 mL of a 2.5 mM hydrogen peroxide solution and 0.02 mL of a 2.5 mM iron (II) chloride solution were added.
h, heated at 37 ° C. The same reaction without the extract, concentrate and purified product was used as a control. After heating, 1
0.01 mL of a 0 mg / mL butylhydroxyl toluene (BHT) ethanol solution was added. Dissolve 12 mg of TBA and 16.2 mg of SDS in 2.3 mL of distilled water,
To this, 20% (v / v) acetate buffer (pH 4.0) was added.
Add 5 mL and 0.2 mL of the above reaction solution, and add
Warmed for hours. After cooling, the absorbance at (532 nm) was measured. The absorbance of the reaction solution containing each sample was D, and the absorbance of the control was E, and the hydroxy radical scavenging rate was calculated from the following <Equation 5>. The higher the hydroxyl radical scavenging rate, the stronger the hydroxyl radical scavenging action.

[0115]

<Equation 5> Hydroxy radical scavenging rate (%) =
{1- (ED) / E} × 100

When the hydroxy radical scavenging rate (%) is 5
The concentration of the measurement object at 0% is compared, and the strength of the hydroxy radical scavenging activity is compared.

[0117]

[Table 2]

From Table 2, it can be seen that the present extract also has the same level of hydroxyl radical scavenging activity as the extract from sesame seeds, which is considered to have strong hydroxy radical scavenging activity. Further, comparing the superoxide scavenging activity of the extract from the sesame seed with the superoxide scavenging activity of the present extract is about 8 to 9 times. Thus, it can be seen that the present extract is an extract having both excellent superoxide scavenging activity and hydroxy radical scavenging activity and an excellent active oxygen removing function.

Next, the method of evaluating the obtained antioxidant effect and the results of evaluation when the present extract is actually applied to products, animals and humans will be described.

First, regarding the antioxidant effect, evaluation methods and evaluation results are shown for the antioxidant effect when blended in actual foods and the in vivo antioxidant effect when actually taken by animals.

Example 18 Evaluation of Antioxidant Effect Using Cookies Ingredients were mixed in the following composition, divided into 10 g pieces, and baked at 180 ° C. for 15 minutes to produce cookies. The control was produced without adding the present extract.

For a constant mass of the cookie immediately after making,
After extracting lipid with hexane and distilling off hexane in accordance with a conventional method, the POV of the lipid was measured. The POV of the control cookie was 2.1, and the POV of the cookie containing the extract was 2. 0.3. 60 of these cookies
After storage in a dark place at 18 ° C. for 18 days, POV was measured in the same manner. The POV of the control cookie was increased to 31.7, whereas the POV of the cookie containing the present extract was 7.5. The cookies obtained in the same manner were preserved in a dark place at 60 ° C. for 18 days and then tasted. As a result, the control cookie had a considerable oxidative deterioration smell, whereas the cookie containing the extract was
Even after storage, it had a good flavor.

From these results, it was found that the present extract has an antioxidant effect and an effect of improving the storage stability of products and the like.

The in vivo antioxidant effect of the olive extract obtained in each Example when actually ingested by an animal was examined.
The evaluation method and the evaluation result will be described.

Example 19 Evaluation of In Vivo Antioxidant Effect Using Rats 6-week-old Wistar male rats (Japan SLC) 7
The animals were set as one test plot, and the powdered purified feed for each test plot was freely taken with water for 4 weeks. Each test group feed was prepared according to the feed composition shown in Table 3 below. The feed was changed once every two days to prevent oxidative degradation of the feed components. Test meal administration 4
The animals were fasted at 9 am in the week and the livers of each rat were collected at 2 pm. The antioxidant activity in the liver was measured using the following AOA method, and the in vivo antioxidant activity of each olive extract of the specimen was evaluated.

[0126]

[Table 3] *: Excluding tocopherol.

<AOA Method (Measurement Method of Lipid Peroxidation Inhibition Rate)
> Prepare a ground buffer of bovine brain phosphate buffer as a substrate. To this is added a triturated liver sample collected by the method described below, and the mixture is kept at 37 ° C. for 1 hour to promote lipid oxidation. A TBA (thiobarbituric acid) solution is added to the deproteinized supernatant of the reaction solution, stirred, and kept at 95 ° C. for 15 minutes. The absorbance of the reaction solution is measured with a spectrophotometer. The magnitude of the absorbance value and the magnitude of the lipid peroxide concentration are correlated. The absorbance of the sample (each olive extract) -added group is compared with the absorbance of the control group, and the lipid peroxidation inhibition rate (%) of the sample (each olive extract) is determined.

The antioxidant effect in vivo was evaluated by the above method. Table 4 shows the results.

[0129]

[Table 4] Liver lipid peroxidation inhibition rate

From Table 4, it can be seen that this extract was ingested
It was confirmed that it exerts its antioxidant effect in vivo and suppresses the production of lipid peroxide resulting from oxidation in vivo. In addition, since this effect is content-dependent, it was found that the content should be increased or decreased according to the desired effect.

Example 20 Tablet (Production Example 1 of Antioxidant) (1) Extract of Example 17 10.0 mg (2) Lactose 94.0 mg (3) Corn Starch 34.0 mg (4) Crystalline cellulose 20 1.0 mg (5) Magnesium stearate 1.0 mg At the above mixing ratio, (1) to (4) were mixed well, and then (5) was added and further mixed. The mixture was tableted to give tablets.

Example 21 Powder (Production Example 2 of Antioxidant) (1) Extract of Example 17 10.0 mg (2) Lactose 981.0 mg (3) Hydroxypropylcellulose 4.0 mg (4) Soft anhydrous 5.0 mg of silicic acid At the above mixing ratio, (1) and (2) are mixed well, and then (3) is added and granulated. This is granulated after drying,
(4) was added and mixed well to obtain a powder.

Example 22 Capsule (Production Example 3 of Antioxidant) (1) Extract of Example 17 10.0 mg (2) Lactose 70.0 mg (3) Maize starch 38.0 mg (4) Stearic acid Magnesium 2.0 mg (5) Capsules 260.0 mg At the above mixing ratio, first, a mixed powder obtained by thoroughly mixing (1) to (4) was filled in (5) to obtain a capsule.

The evaluation method and evaluation criteria for the lipid peroxides-suppressing effect on the in vivo antioxidant effect when the tablet obtained in Example 20 was actually taken by a human are shown below.

Example 23 Evaluation of in vivo antioxidant effect on human body Evaluation of in vivo antioxidant effect when the tablet obtained in Example 20 was used for humans was evaluated by blindly examining 30 healthy males in their 30s. I went in. That is, 30 males in their thirties were examined for 15 times so that the average blood lipid peroxide concentration before the test became equal.
The test group and the control group were divided into two groups, each of which had a tablet containing the olive extract of Example 17 for the test group, and Example 17 for the control group. Tablets containing no olive extract were taken at the same time as each meal (morning, noon, evening). The test was performed for 30 days, and blood was collected every 10 days. Serum was prepared from blood by centrifugation and stored at -80 ° C until measurement. The amount of lipid peroxide (PCOOH) in the serum was measured by the following method, and the in vivo antioxidant effect of the tablet obtained in Example 20 was evaluated from the obtained results.

<Method of Measuring Lipid Peroxide (PCOOH)> The amount of PCOOH in serum was measured according to the method of Oshima et al. (Lipids, 31, 1091, 1996). That is, a solution of chloroform-methanol (2/1 (v / v)) containing 20 ppm BHT and NBD-
A chloroform solution of PC was added, the mixture was shaken well, and then centrifuged to collect a chloroform layer. After distilling off the solvent, the solution dissolved in a mobile phase (chloroform: methanol: water = 9: 50: 1) used for HPLC was subjected to post-column HPLC using diphenylpyrenylphosphine (DPPP) as a reaction reagent. .

In the above-mentioned evaluation test, the antioxidant effect in a human body was evaluated. Table 5 shows the results of the change over time in the amount of lipid peroxide (PCOOH) in serum in each section.

[0138]

[Table 5] Time course of serum lipid peroxide (PCOOH) level

From Table 5, it can be seen that the antioxidant containing the olive extract of the present invention exerts its antioxidant effect in vivo when ingested by humans and acts as an antioxidant in vivo.
It was confirmed that the production of lipid peroxide resulting from oxidation in vivo was significantly suppressed.

[0140]

According to the present invention, an antioxidant having an excellent antioxidant effect and containing an extract having an active oxygen removing function from olive plants and the like can be obtained. According to the present invention, it is possible to stably provide an antioxidant containing a natural extract having a function of removing active oxygen such as strong superoxide elimination or hydroxy radical elimination. The antioxidant of the present invention has a strong antioxidant effect in a trace amount, by using the antioxidant in the human body and other foods and drinks, pharmaceuticals, fertilizers, feed and external preparations for skin, etc., the antioxidant effect, In particular, an excellent antioxidant effect such as an effect of improving the storage stability of the product, and an excellent antioxidant effect in vivo such as an effect of preventing various diseases and an effect of antiaging can be obtained. Further, according to the present invention, since the extract used for the antioxidant of the present invention can be obtained with a high yield, the total amount (total index) of the effects that can be obtained per unit raw material is very large. further,
It can be easily obtained from olive plants, and can also be suitably obtained from normally discarded products generated during the process of producing olive oil.From the viewpoint of stable supply and cost, It is preferable from the viewpoint of effective use of resources.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A61P 39/06 A61P 39/06

Claims (19)

[Claims] 1. Olive plant (excluding leaves; the same applies hereinafter)
An antioxidant comprising, as an active ingredient, an extract obtained from the above. 2. The extract according to claim 1, wherein the extract is obtained by extracting a product obtained in an olive plant and / or olive oil production process with water and / or an organic solvent.
An antioxidant according to the above. 3. The antioxidant according to claim 2, wherein the extract is further subjected to a concentration treatment and / or a fractionation / purification treatment. 4. An antioxidant comprising an extract obtained from an olive plant as an active ingredient. 5. The antioxidant according to claim 4, which has an effect of improving storage stability. 6. An extract obtained by subjecting a product obtained in an olive plant and / or olive oil production process to an extraction treatment with water and / or an organic solvent.
Or the antioxidant according to 5. 7. The antioxidant according to claim 6, wherein the extract has been further subjected to a concentration treatment and / or a fractionation / purification treatment. 8. An in vivo antioxidant comprising an extract obtained from an olive plant as an active ingredient. 9. The in vivo antioxidant according to claim 8, which has various disease preventing effects and / or anti-aging effects. 10. The in vivo organism according to claim 8, wherein the extract is obtained by extracting a product obtained in an olive plant and / or olive oil production process with water and / or an organic solvent. Antioxidants. 11. The antioxidant in vivo according to claim 10, wherein the extract has been further subjected to concentration treatment and / or fractionation / purification treatment. 12. The composition according to claim 1, which is easily soluble in water.
The antioxidant, antioxidant or in vivo antioxidant according to any one of claims 1 to 7. 13. The antioxidant, the antioxidant or the antioxidant according to any one of claims 1 to 11, wherein a part or the whole thereof is dissolved in water or a water-containing hydrophilic organic solvent. In vivo antioxidants. 14. An antioxidant, an antioxidant, or an antioxidant, wherein the antioxidant, the antioxidant, or the in vivo antioxidant according to any one of claims 1 to 13 is blended in various products. To use an antioxidant or in vivo antioxidant. 15. The use according to claim 14, wherein the product is a food or drink. 16. An antioxidant, an antioxidant, or a bioactive agent, which is characterized in that the antioxidant, the antioxidant, or the in vivo antioxidant according to any one of claims 1 to 13 is taken therein. How to use antioxidants in the body. 17. Use of an extract obtained from olives as an antioxidant. 18. Use of an extract obtained from olives as an antioxidant. 19. A method for using an extract obtained from olives as an in vivo antioxidant.