JP2001501097A - レセプターエフェクターを同定する方法及び組成物 - Google Patents

レセプターエフェクターを同定する方法及び組成物

Info

Publication number
JP2001501097A
JP2001501097A JP10515880A JP51588098A JP2001501097A JP 2001501097 A JP2001501097 A JP 2001501097A JP 10515880 A JP10515880 A JP 10515880A JP 51588098 A JP51588098 A JP 51588098A JP 2001501097 A JP2001501097 A JP 2001501097A
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JP
Japan
Prior art keywords
yeast
assay
receptor
gene
protein
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP10515880A
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English (en)
Japanese (ja)
Other versions
JP2001501097A5 (enExample
Inventor
トルーハート，ジョシュア
アイ． ポール，ジェレミー
エイ． フエルンクランツ，ハンス
ネイサン，デブラ
ホームズ，スコット
Original Assignee
ケイダス ファーマスーティカル コーポレイション
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Application filed by ケイダス ファーマスーティカル コーポレイション filed Critical ケイダス ファーマスーティカル コーポレイション
Publication of JP2001501097A publication Critical patent/JP2001501097A/ja
Publication of JP2001501097A5 publication Critical patent/JP2001501097A5/ja
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP10515880A 1996-09-24 1997-09-24 レセプターエフェクターを同定する方法及び組成物 Ceased JP2001501097A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US71891096A 1996-09-24 1996-09-24
US85146997A 1997-05-05 1997-05-05
US08/718,910 1997-05-05
US08/851,469 1997-05-05
PCT/US1997/017159 WO1998013513A2 (en) 1996-09-24 1997-09-24 Methods and compositions for identifying receptor effectors

Publications (2)

Publication Number Publication Date
JP2001501097A true JP2001501097A (ja) 2001-01-30
JP2001501097A5 JP2001501097A5 (enExample) 2005-05-12

Family

ID=27109999

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10515880A Ceased JP2001501097A (ja) 1996-09-24 1997-09-24 レセプターエフェクターを同定する方法及び組成物

Country Status (8)

Country Link
US (1) US6159705A (enExample)
EP (1) EP0929691B1 (enExample)
JP (1) JP2001501097A (enExample)
AT (1) ATE284972T1 (enExample)
AU (1) AU733007B2 (enExample)
CA (1) CA2266543C (enExample)
DE (1) DE69731960T2 (enExample)
WO (1) WO1998013513A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008245592A (ja) * 2007-03-30 2008-10-16 As One Corp 自己リン酸化受容体のアゴニスト、アンタゴニストのスクリーニング方法、遺伝子組換え酵母
JP2019506150A (ja) * 2015-12-22 2019-03-07 アルブミディクス リミティド 改善されたタンパク質発現株
US11130979B2 (en) 2017-06-20 2021-09-28 Albumedix Ltd Protein expression strains

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6255059B1 (en) 1993-03-31 2001-07-03 Cadus Pharmaceutical Corporation Methods for identifying G protein coupled receptor effectors
US6100042A (en) 1993-03-31 2000-08-08 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US7235648B1 (en) 1993-03-31 2007-06-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US20030054402A1 (en) 1993-03-31 2003-03-20 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
US6864060B1 (en) 1993-03-31 2005-03-08 Cadus Technologies, Inc. Yeast cells expressing modified G proteins and methods of use therefor
US7416881B1 (en) 1993-03-31 2008-08-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US6403305B1 (en) * 1997-02-06 2002-06-11 Cornell Research Foundation, Inc. Methods of identifying peptide agonists or negative antagonists of a G protein coupled receptor
US6555339B1 (en) * 1997-04-14 2003-04-29 Arena Pharmaceuticals, Inc. Non-endogenous, constitutively activated human protein-coupled receptors
US7119190B2 (en) * 1997-04-14 2006-10-10 Arena Pharmaceuticals, Inc. Endogenous and non-endogenous versions of human G protein-coupled receptors
AU743259B2 (en) * 1997-04-14 2002-01-24 Arena Pharmaceuticals, Inc. A method of identifying modulators of cell surface membrane receptors useful in the treatment of disease
AU8142598A (en) 1997-06-11 1998-12-30 New York University Prenylcysteine carboxyl methyltransferase, dna encoding same, and a method of screening for inhibitors thereof
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
AU9791198A (en) * 1997-10-07 1999-04-27 Cadus Pharmaceutical Corporation Yeast cells expressing modified g proteins and methods of use therefor
US6355473B1 (en) 1998-05-06 2002-03-12 Cadus Pharmaceutical Corp. Yeast cells having mutations in stp22 and uses therefor
US20020015967A1 (en) * 1998-06-02 2002-02-07 Lauren Silverman Functional expression of adenosine receptors in yeast
FR2780512B1 (fr) * 1998-06-25 2003-10-17 Lipha Utilisation de recepteurs de la famille rev-erb pour le criblage de substances utiles dans le traitement des dysfonctionnements du metabolisme lipidique
US7081360B2 (en) 1998-07-28 2006-07-25 Cadus Technologies, Inc. Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties
WO2000006722A1 (en) * 1998-07-28 2000-02-10 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Eukaryotic cell-based gene interaction cloning
US6472151B1 (en) * 1998-08-19 2002-10-29 Bristol-Myers Squibb Company Method of screening for compounds that modulate the activity of a molecular target
US7273747B2 (en) 1998-08-27 2007-09-25 Cadus Technologies, Inc. Cell having amplified signal transduction pathway responses and uses therefor
US6251605B1 (en) 1998-10-27 2001-06-26 Cadus Pharmaceutical Corporation Yeast cells having mutations in Cav1 and uses therefor
AU2030000A (en) * 1998-11-25 2000-06-13 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
DE19860833C1 (de) * 1998-12-30 2000-09-07 Albrecht E Sippel Methode zur zellulären High-Throughput(Hochdurchsatz)-Detektion von Rezeptor-Liganden-Interaktionen
US6504008B1 (en) * 1999-02-01 2003-01-07 Cadus Technologies, Inc. Cell based signal generation
US6555325B1 (en) 1999-06-14 2003-04-29 Cadus Technologies, Inc. System for detection of a functional interaction between a compound and a cellular signal transduction component
US7742877B1 (en) * 1999-07-22 2010-06-22 Becton, Dickinson & Company Methods, apparatus and computer program products for formulating culture media
US7223533B2 (en) 1999-09-10 2007-05-29 Cadus Technologies, Inc. Cell surface proteins and use thereof as indicators of activation of cellular signal transduction pathways
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
JP2004520824A (ja) * 2000-12-01 2004-07-15 ワイス Ｒｇｓタンパク質のモジュレーターを検出するための方法および細胞
GB0030038D0 (en) * 2000-12-08 2001-01-24 Univ Warwick Yeast-based assay
JP2004530117A (ja) * 2001-02-22 2004-09-30 プラエシス ファーマシューティカルズ インコーポレーテッド 生物プロセスを調節するペプチドを同定する方法
CA2439383A1 (en) * 2001-02-26 2002-09-06 Arena Pharmaceuticals, Inc. Endogenous and non-endogenous versions of human g protein-coupled receptors
EP1409656A4 (en) * 2001-03-12 2005-01-12 Darley Pharmaceuticals Ltd SCREENING OF PHEROMONAL COMPOUNDS OF NON-STEROID AND NON-PEPTIDE INVERTEBRATE HAVING ACTIVITY MODULATING MITOGEN-ACTIVATED PROTEIN KINASE
US20070003980A1 (en) * 2001-03-27 2007-01-04 Woods Daniel F Efficient methods to isolate effectors of proteins involved in olfactory or chemosensory pathways and efficient methods to use these effectors to alter organism olfaction, chemosensation, or behavior
US20100248268A1 (en) * 2001-03-27 2010-09-30 Woods Daniel F Methods to utilize invertebrate chemosensory proteins for industrial and commercial uses
CA2442581A1 (en) * 2001-03-27 2002-10-03 Inscent, Inc. Efficient methods for isolating functional g-protein coupled receptors and identifying active effectors and efficient methods to isolate proteins involved in olfaction and efficient methods to isolate and identifying active effectors
JP4109123B2 (ja) * 2001-05-09 2008-07-02 セルメド オンコロジー （ユーエスエイ）， インコーポレイテッド ペプチドデホルミラーゼ活性化プロドラッグ
AU2002317012A1 (en) * 2001-06-28 2003-03-03 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Cellular reporter gene assay with a ligand amplifying feedback loop
US6833441B2 (en) 2001-08-01 2004-12-21 Abmaxis, Inc. Compositions and methods for generating chimeric heteromultimers
NZ513755A (en) * 2001-08-24 2001-09-28 Ann Rachel Holmes Protein expression system in yeast comprising a vector encoding a heterologous membrane protein and its application in screening for drugs
US20060105377A1 (en) * 2001-08-30 2006-05-18 Eglen Richard M Screening to optimize RNAi
EP1421385B1 (en) * 2001-08-30 2016-06-29 DiscoveRx Corporation Genetic construct intracellular monitoring system
US7358043B2 (en) 2001-10-16 2008-04-15 Duke University Proteomic interaction and genomic action determinations in the presence of associated redox state conditions
US7175983B2 (en) * 2001-11-02 2007-02-13 Abmaxis, Inc. Adapter-directed display systems
EP1515985B1 (en) 2002-01-23 2013-01-02 Mohammed Raafat El-Gewely Methods of screening molecular libraries for active molecules that restore the function of mutant p53
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US7229762B2 (en) * 2002-10-15 2007-06-12 Duke University Medical Center Proteomic screening for redox state dependent protein—protein interactions
EP1585807A4 (en) * 2002-12-23 2006-05-24 Glycofi Inc STEROID RECEPTOR MODULATION OF GENE EXPRESSION
US20040209246A1 (en) * 2003-04-15 2004-10-21 Agouron Pharmaceuticals, Inc. Dual reporter/dye reduction methodology for evaluating antiviral and cytotoxicity of hepatitis C virus inhibitors
US7718363B2 (en) * 2003-04-25 2010-05-18 The Kenneth S. Warren Institute, Inc. Tissue protective cytokine receptor complex and assays for identifying tissue protective compounds
US20050112551A1 (en) * 2003-11-24 2005-05-26 Agouron Pharmaceuticals, Inc. Dual assay for evaluating activity and cytotoxicity of compounds in the same population of cells
EP1689348B1 (en) 2003-12-05 2013-05-15 Children's Hospital Medical Center Oligosaccharide compositions and use thereof in the treatment of infection
CA3187687A1 (en) 2007-09-14 2009-03-19 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20090203149A1 (en) * 2008-02-13 2009-08-13 General Electric Company Enhanced methods for gas and/or vapor phase analysis of biological assays
DE102008064553B4 (de) * 2008-12-22 2015-05-13 Technische Universität Dresden Verfahren zum Nachweis und/oder zur Identifizierung hormonell wirksamer Substanzen
EP3336225B1 (en) 2010-07-16 2020-02-19 Adimab, LLC Antibody libraries
WO2017112847A1 (en) * 2015-12-22 2017-06-29 Albumedix A/S Improved protein expression strains
JP2019510510A (ja) 2016-02-24 2019-04-18 アロマイクス・コーポレーション 臭い（ｓｍｅｌｌ）、香り（ｓｃｅｎｔ）および食味（ｔａｓｔｅ）を検出するためのバイオセンサー
US10209239B1 (en) 2017-08-16 2019-02-19 Aromyx Corporation Method of making an aromagraph comprising ectopic olfactory receptors
CN110904123B (zh) * 2019-01-07 2021-11-05 广西科学院 一种用于促进高产甘蔗糖蜜酒精发酵的菌株及其应用
WO2025008607A1 (en) * 2023-07-06 2025-01-09 Imperial College Innovations Limited Determining the effects of modified peptides

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4613572A (en) * 1983-08-15 1986-09-23 Kansas State University Research Foundation Yeast BAR1 gene plasmid
US4880734A (en) * 1984-05-11 1989-11-14 Chiron Corporation Eukaryotic regulatable transcription
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
WO1988010308A1 (en) * 1987-06-24 1988-12-29 Whitehead Institute For Biomedical Research Pheromone-inducible yeast promoter
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
EP0428000A1 (en) * 1989-11-03 1991-05-22 Abbott Laboratories Fluorogenic substrates for the detection of proteolytic enzyme activity
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
DK93990D0 (da) * 1990-04-17 1990-04-17 Carlsberg As Fluorogene peptider og deres anvendelse ved bestemmelse af enzymatisk aktivitet
US5401629A (en) * 1990-08-07 1995-03-28 The Salk Institute Biotechnology/Industrial Associates, Inc. Assay methods and compositions useful for measuring the transduction of an intracellular signal
WO1992005244A1 (en) * 1990-09-13 1992-04-02 Duke University Expression of g protein coupled receptors in yeast
EP1362913B1 (en) * 1992-10-30 2006-01-25 The General Hospital Corporation Interaction trap system for isolating proteins
WO1994016100A1 (en) * 1993-01-05 1994-07-21 The Salk Institute For Biological Studies Functional assays for the identification of ligands which modulate signal transduction
EP0915154A1 (en) * 1993-03-31 1999-05-12 Cadus Pharmaceuticals, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US5876951A (en) * 1993-03-31 1999-03-02 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates and uses therefor
US5571688A (en) * 1993-05-06 1996-11-05 Mekalanos; John J. Method of detecting gene expression
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995021191A1 (en) * 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
CA2188950C (en) * 1994-04-26 2001-07-03 James R. Broach Functional expression of mammalian adenylyl cyclase in yeast
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
GB9502830D0 (en) * 1995-02-14 1995-04-05 Ecole Polytech Regulation of polypeptide production in cells
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
US5610015A (en) * 1995-03-23 1997-03-11 Wisconsin Alumni Research Foundation System to detect protein-RNA interactions
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5569588A (en) * 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008245592A (ja) * 2007-03-30 2008-10-16 As One Corp 自己リン酸化受容体のアゴニスト、アンタゴニストのスクリーニング方法、遺伝子組換え酵母
JP2019506150A (ja) * 2015-12-22 2019-03-07 アルブミディクス リミティド 改善されたタンパク質発現株
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
US12371457B2 (en) 2015-12-22 2025-07-29 Sartorius Albumedix Limited Protein expression strains
US11130979B2 (en) 2017-06-20 2021-09-28 Albumedix Ltd Protein expression strains
US11396670B2 (en) 2017-06-20 2022-07-26 Albumedix Limited Protein expression strains

Also Published As

Publication number Publication date
DE69731960T2 (de) 2005-12-15
WO1998013513A2 (en) 1998-04-02
DE69731960D1 (de) 2005-01-20
ATE284972T1 (de) 2005-01-15
AU4593097A (en) 1998-04-17
EP0929691A2 (en) 1999-07-21
CA2266543A1 (en) 1998-04-02
WO1998013513A3 (en) 1998-09-03
CA2266543C (en) 2003-12-09
US6159705A (en) 2000-12-12
EP0929691B1 (en) 2004-12-15
AU733007B2 (en) 2001-05-03

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