JP2000512153A - グリセルアルデヒド３リン酸脱水素酵素および細胞質性雄性不稔の核性回復 - Google Patents
グリセルアルデヒド３リン酸脱水素酵素および細胞質性雄性不稔の核性回復Info
- Publication number
- JP2000512153A JP2000512153A JP10501998A JP50199898A JP2000512153A JP 2000512153 A JP2000512153 A JP 2000512153A JP 10501998 A JP10501998 A JP 10501998A JP 50199898 A JP50199898 A JP 50199898A JP 2000512153 A JP2000512153 A JP 2000512153A
- Authority
- JP
- Japan
- Prior art keywords
- gene
- nuclear
- male sterility
- cytoplasmic male
- recovery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010021929 Infertility male Diseases 0.000 title claims abstract description 26
- 208000007466 Male Infertility Diseases 0.000 title claims abstract description 26
- 230000001086 cytosolic effect Effects 0.000 title claims abstract description 26
- OWEFQTXQEHYDEJ-UHFFFAOYSA-N 2,3-dihydroxypropanal diphosphono hydrogen phosphate Chemical compound OCC(O)C=O.OP(O)(=O)OP(O)(=O)OP(O)(O)=O OWEFQTXQEHYDEJ-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 101710088194 Dehydrogenase Proteins 0.000 title claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 82
- 238000011084 recovery Methods 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 32
- 230000002068 genetic effect Effects 0.000 claims abstract description 16
- 239000002299 complementary DNA Substances 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims description 24
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 22
- 239000012634 fragment Substances 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims 3
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims 3
- 230000002093 peripheral effect Effects 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 230000009466 transformation Effects 0.000 abstract description 9
- 239000003550 marker Substances 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 description 45
- 108020004414 DNA Proteins 0.000 description 19
- 101150093511 GAPC gene Proteins 0.000 description 18
- 101150115724 GAPC1 gene Proteins 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 108020004635 Complementary DNA Proteins 0.000 description 9
- 240000002791 Brassica napus Species 0.000 description 8
- 238000010804 cDNA synthesis Methods 0.000 description 8
- 210000000805 cytoplasm Anatomy 0.000 description 7
- 150000007523 nucleic acids Chemical group 0.000 description 7
- 235000013339 cereals Nutrition 0.000 description 6
- 235000011293 Brassica napus Nutrition 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 4
- 230000035558 fertility Effects 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 241000589158 Agrobacterium Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 101150094969 rfp1 gene Proteins 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 240000000385 Brassica napus var. napus Species 0.000 description 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 241000831652 Salinivibrio sharmensis Species 0.000 description 2
- 240000006394 Sorghum bicolor Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 235000007230 Sorghum bicolor Nutrition 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000010397 one-hybrid screening Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2055396P | 1996-06-26 | 1996-06-26 | |
| US60/020,553 | 1996-06-26 | ||
| PCT/CA1997/000424 WO1997049831A1 (en) | 1996-06-26 | 1997-06-16 | Glyceraldehyde-3-phosphate dehydrogenase and nuclear restoration of cytoplasmic male sterility |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2000512153A true JP2000512153A (ja) | 2000-09-19 |
Family
ID=21799249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10501998A Pending JP2000512153A (ja) | 1996-06-26 | 1997-06-16 | グリセルアルデヒド３リン酸脱水素酵素および細胞質性雄性不稔の核性回復 |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0954604A1 (hu) |
| JP (1) | JP2000512153A (hu) |
| CN (1) | CN1228126A (hu) |
| AU (1) | AU732094B2 (hu) |
| CA (1) | CA2258561C (hu) |
| CZ (1) | CZ424098A3 (hu) |
| HU (1) | HUP9904008A3 (hu) |
| PL (1) | PL330793A1 (hu) |
| WO (1) | WO1997049831A1 (hu) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002088179A1 (fr) * | 2001-04-25 | 2002-11-07 | Mitsubishi Chemical Corporation | Proteine participant a la restauration de fertilite de la sterilite male cytoplasmique et gene codant ladite proteine |
| EP1404814B1 (en) * | 2001-07-12 | 2013-06-19 | McGill University | Nuclear fertility restorer genes and methods of use in plants |
| US7314971B2 (en) | 2001-07-12 | 2008-01-01 | Basf Plant Science Gmbh | Nuclear fertility restorer genes and methods of use in plants |
| AU2008202565B2 (en) * | 2002-07-12 | 2012-04-12 | Basf Plant Science Gmbh | Nuclear fertility restorer genes and methods of use in plants |
-
1997
- 1997-06-16 CN CN97197340A patent/CN1228126A/zh active Pending
- 1997-06-16 CZ CZ984240A patent/CZ424098A3/cs unknown
- 1997-06-16 CA CA002258561A patent/CA2258561C/en not_active Expired - Fee Related
- 1997-06-16 WO PCT/CA1997/000424 patent/WO1997049831A1/en not_active Application Discontinuation
- 1997-06-16 AU AU30857/97A patent/AU732094B2/en not_active Ceased
- 1997-06-16 PL PL97330793A patent/PL330793A1/xx unknown
- 1997-06-16 HU HU9904008A patent/HUP9904008A3/hu unknown
- 1997-06-16 JP JP10501998A patent/JP2000512153A/ja active Pending
- 1997-06-16 EP EP97925801A patent/EP0954604A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| PL330793A1 (en) | 1999-06-07 |
| HUP9904008A2 (hu) | 2000-04-28 |
| AU3085797A (en) | 1998-01-14 |
| CZ424098A3 (cs) | 1999-09-15 |
| CN1228126A (zh) | 1999-09-08 |
| HUP9904008A3 (en) | 2001-10-29 |
| WO1997049831A1 (en) | 1997-12-31 |
| CA2258561A1 (en) | 1997-12-31 |
| CA2258561C (en) | 2009-09-01 |
| AU732094B2 (en) | 2001-04-12 |
| EP0954604A1 (en) | 1999-11-10 |
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