JP2000511562A - 枝分れしたオリゴマーの合成のための多官能性結合試薬 - Google Patents
枝分れしたオリゴマーの合成のための多官能性結合試薬Info
- Publication number
- JP2000511562A JP2000511562A JP10531144A JP53114498A JP2000511562A JP 2000511562 A JP2000511562 A JP 2000511562A JP 10531144 A JP10531144 A JP 10531144A JP 53114498 A JP53114498 A JP 53114498A JP 2000511562 A JP2000511562 A JP 2000511562A
- Authority
- JP
- Japan
- Prior art keywords
- group
- reagent
- individually
- nucleotide
- alkyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
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- 239000002202 Polyethylene glycol Substances 0.000 description 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
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- 150000007513 acids Chemical class 0.000 description 1
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- 125000003275 alpha amino acid group Chemical group 0.000 description 1
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- 150000001501 aryl fluorides Chemical class 0.000 description 1
- 238000002820 assay format Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- PASDCCFISLVPSO-UHFFFAOYSA-N benzoyl chloride Chemical compound ClC(=O)C1=CC=CC=C1 PASDCCFISLVPSO-UHFFFAOYSA-N 0.000 description 1
- 235000019445 benzyl alcohol Nutrition 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 150000001615 biotins Chemical class 0.000 description 1
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- 239000003593 chromogenic compound Substances 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
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- 230000005494 condensation Effects 0.000 description 1
- 229930189851 creoside Natural products 0.000 description 1
- HPXRVTGHNJAIIH-UHFFFAOYSA-N cyclohexanol Chemical compound OC1CCCCC1 HPXRVTGHNJAIIH-UHFFFAOYSA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
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- 239000007850 fluorescent dye Substances 0.000 description 1
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- 125000005179 haloacetyl group Chemical group 0.000 description 1
- LNEPOXFFQSENCJ-UHFFFAOYSA-N haloperidol Chemical compound C1CC(O)(C=2C=CC(Cl)=CC=2)CCN1CCCC(=O)C1=CC=C(F)C=C1 LNEPOXFFQSENCJ-UHFFFAOYSA-N 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 150000002466 imines Chemical class 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229940087646 methanolamine Drugs 0.000 description 1
- ZKQZJMVQJMMAFG-UHFFFAOYSA-N methanolate thorium(4+) Chemical compound [Th+4].C[O-].C[O-].C[O-].C[O-] ZKQZJMVQJMMAFG-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- QUSNBJAOOMFDIB-UHFFFAOYSA-N monoethyl amine Natural products CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000000269 nucleophilic effect Effects 0.000 description 1
- 239000008601 oleoresin Substances 0.000 description 1
- AICOOMRHRUFYCM-ZRRPKQBOSA-N oxazine, 1 Chemical compound C([C@@H]1[C@H](C(C[C@]2(C)[C@@H]([C@H](C)N(C)C)[C@H](O)C[C@]21C)=O)CC1=CC2)C[C@H]1[C@@]1(C)[C@H]2N=C(C(C)C)OC1 AICOOMRHRUFYCM-ZRRPKQBOSA-N 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- PTMHPRAIXMAOOB-UHFFFAOYSA-N phosphoramidic acid Chemical compound NP(O)(O)=O PTMHPRAIXMAOOB-UHFFFAOYSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- OENLEHTYJXMVBG-UHFFFAOYSA-N pyridine;hydrate Chemical compound [OH-].C1=CC=[NH+]C=C1 OENLEHTYJXMVBG-UHFFFAOYSA-N 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000012857 radioactive material Substances 0.000 description 1
- 235000005828 ramon Nutrition 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 125000006853 reporter group Chemical group 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000003998 snake venom Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 239000012312 sodium hydride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- AKEJUJNQAAGONA-UHFFFAOYSA-N sulfur trioxide Inorganic materials O=S(=O)=O AKEJUJNQAAGONA-UHFFFAOYSA-N 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/780,725 US5916750A (en) | 1997-01-08 | 1997-01-08 | Multifunctional linking reagents for synthesis of branched oligomers |
| US08/780,725 | 1997-01-08 | ||
| PCT/US1998/000445 WO1998030719A1 (en) | 1997-01-08 | 1998-01-07 | Multifunctional linking reagents for synthesis of branched oligomers |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004151055A Division JP2004305216A (ja) | 1997-01-08 | 2004-04-19 | 核酸オリゴマー、その製造方法及びそれを製造するためのキット |
| JP2004151054A Division JP2004325457A (ja) | 1997-01-08 | 2004-04-19 | 試料中の検体の存在又は量を検出する方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2000511562A true JP2000511562A (ja) | 2000-09-05 |
Family
ID=25120484
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10531144A Pending JP2000511562A (ja) | 1997-01-08 | 1998-01-07 | 枝分れしたオリゴマーの合成のための多官能性結合試薬 |
| JP2004151054A Pending JP2004325457A (ja) | 1997-01-08 | 2004-04-19 | 試料中の検体の存在又は量を検出する方法 |
| JP2004151055A Pending JP2004305216A (ja) | 1997-01-08 | 2004-04-19 | 核酸オリゴマー、その製造方法及びそれを製造するためのキット |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004151054A Pending JP2004325457A (ja) | 1997-01-08 | 2004-04-19 | 試料中の検体の存在又は量を検出する方法 |
| JP2004151055A Pending JP2004305216A (ja) | 1997-01-08 | 2004-04-19 | 核酸オリゴマー、その製造方法及びそれを製造するためのキット |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5916750A (https=) |
| EP (1) | EP1019534A1 (https=) |
| JP (3) | JP2000511562A (https=) |
| AU (1) | AU5912098A (https=) |
| CA (1) | CA2276481A1 (https=) |
| WO (1) | WO1998030719A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009008673A (ja) * | 2000-11-09 | 2009-01-15 | Beckman Coulter Inc | シアニン色素フォスフォアミダイト |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020022261A1 (en) * | 1995-06-29 | 2002-02-21 | Anderson Rolfe C. | Miniaturized genetic analysis systems and methods |
| JP4004230B2 (ja) * | 1998-11-06 | 2007-11-07 | 株式会社三菱化学ヤトロン | 架橋アビジン含有新規複合体、架橋アビジンを用いる分析方法、並びに分析用試薬及びキット |
| US7135284B1 (en) | 1999-02-05 | 2006-11-14 | Integrated Dna Technologies, Inc. | Primer extension methods for production of high specific activity nucleic acid probes |
| US6258593B1 (en) * | 1999-06-30 | 2001-07-10 | Agilent Technologies Inc. | Apparatus for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber |
| EP1244812A1 (en) * | 1999-12-21 | 2002-10-02 | LION Bioscience AG | Branched compound for use in nucleic acid detection and analysis reactions |
| EP1111068A1 (en) * | 1999-12-21 | 2001-06-27 | LION Bioscience AG | Branched compound for use in nucleic acid detection and analysis reactions |
| GB0016813D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (4) |
| GB0102388D0 (en) * | 2001-01-31 | 2001-03-14 | Secr Defence | Polymers |
| CA2439477C (en) * | 2001-02-27 | 2014-04-15 | Virco Bvba | Circular probe amplification (cpa) using energy-transfer primers |
| ATE432347T1 (de) * | 2002-06-24 | 2009-06-15 | Exiqon As | Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen |
| EP3257949A1 (en) | 2005-06-15 | 2017-12-20 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| CA2624896C (en) | 2005-10-07 | 2017-11-07 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
| US7960104B2 (en) * | 2005-10-07 | 2011-06-14 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
| SG170028A1 (en) * | 2006-02-24 | 2011-04-29 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
| CN101432439B (zh) * | 2006-02-24 | 2013-07-24 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
| US7910354B2 (en) * | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20090111706A1 (en) * | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by amplification |
| CA2690612C (en) | 2007-05-14 | 2016-07-05 | Historx, Inc. | Compartment segregation by pixel characterization using image data clustering |
| ES2599902T3 (es) * | 2007-06-15 | 2017-02-06 | Novartis Ag | Sistema y método de microscopio para obtener datos de muestra normalizados |
| CA2604317C (en) | 2007-08-06 | 2017-02-28 | Historx, Inc. | Methods and system for validating sample images for quantitative immunoassays |
| CA2596204C (en) * | 2007-08-07 | 2019-02-26 | Historx, Inc. | Method and system for determining an optimal dilution of a reagent |
| WO2009029810A1 (en) * | 2007-08-31 | 2009-03-05 | Historx, Inc. | Automatic exposure time selection for imaging tissue |
| WO2009132028A1 (en) * | 2008-04-21 | 2009-10-29 | Complete Genomics, Inc. | Array structures for nucleic acid detection |
| JP5551702B2 (ja) * | 2008-09-16 | 2014-07-16 | ヒストロックス，インコーポレイテッド． | バイオマーカー発現の再現性のある定量 |
| EP2539355B1 (en) * | 2010-02-26 | 2016-10-05 | Ventana Medical Systems, Inc. | In-situ hybridization with polytag probes |
| WO2011117353A1 (en) * | 2010-03-24 | 2011-09-29 | Mirrx Therapeutics A/S | Bivalent antisense oligonucleotides |
| JP2023524812A (ja) * | 2020-05-08 | 2023-06-13 | エムペグ エルエイ リミテッド ライアビリティ カンパニー | リンカー化合物 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3574520A (en) * | 1966-11-02 | 1971-04-13 | Warnaco Inc | Treating cellulosic garments with a s-trimethylol triazine triol,buffered magnesium chloride,and methylol stearamide |
| US5359100A (en) * | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| JPH06505704A (ja) * | 1990-09-20 | 1994-06-30 | ギリアド サイエンシズ，インコーポレイテッド | 改変ヌクレオシド間結合 |
| WO1995001365A1 (en) * | 1993-07-02 | 1995-01-12 | Lynx Therapeutics, Inc. | Synthesis of branched nucleic acids |
-
1997
- 1997-01-08 US US08/780,725 patent/US5916750A/en not_active Expired - Lifetime
-
1998
- 1998-01-07 CA CA002276481A patent/CA2276481A1/en not_active Abandoned
- 1998-01-07 AU AU59120/98A patent/AU5912098A/en not_active Abandoned
- 1998-01-07 JP JP10531144A patent/JP2000511562A/ja active Pending
- 1998-01-07 EP EP98902467A patent/EP1019534A1/en not_active Withdrawn
- 1998-01-07 WO PCT/US1998/000445 patent/WO1998030719A1/en not_active Ceased
-
2004
- 2004-04-19 JP JP2004151054A patent/JP2004325457A/ja active Pending
- 2004-04-19 JP JP2004151055A patent/JP2004305216A/ja active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009008673A (ja) * | 2000-11-09 | 2009-01-15 | Beckman Coulter Inc | シアニン色素フォスフォアミダイト |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1019534A1 (en) | 2000-07-19 |
| AU5912098A (en) | 1998-08-03 |
| JP2004325457A (ja) | 2004-11-18 |
| JP2004305216A (ja) | 2004-11-04 |
| WO1998030719A1 (en) | 1998-07-16 |
| US5916750A (en) | 1999-06-29 |
| CA2276481A1 (en) | 1998-07-16 |
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