JP2000510572A - 免疫蛍光法による調査のための走査毛細管におけるサンプルの調製方法 - Google Patents
免疫蛍光法による調査のための走査毛細管におけるサンプルの調製方法Info
- Publication number
- JP2000510572A JP2000510572A JP08513917A JP51391796A JP2000510572A JP 2000510572 A JP2000510572 A JP 2000510572A JP 08513917 A JP08513917 A JP 08513917A JP 51391796 A JP51391796 A JP 51391796A JP 2000510572 A JP2000510572 A JP 2000510572A
- Authority
- JP
- Japan
- Prior art keywords
- whole blood
- sample
- blood sample
- fluorescent
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Classifications
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Landscapes
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- Immunology (AREA)
- Chemical & Material Sciences (AREA)
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- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Zoology (AREA)
- Dispersion Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 全血液サンプルを提供し; 全血液サンプルを、全血液サンプルの標的成分と選択的に結合するように形 成した蛍光化合物で染色し; 試薬を全血液サンプルへ添加して全血液サンプルにおける赤血球胞の凝集を 阻止し; 赤血球が実質的に標的成分の分類を妨害しない前記励起波長を有する光で蛍 光化合物を励起する; 工程を有する静的血液サンプルにおける標的成分を分類する分析方法。 2. 前記染色工程が蛍光化合物を全血液サンプルの細胞亜群と結合することを含 む請求項1に記載の方法。 3. 前記染色工程が蛍光化合物を白血球と結合することを含む請求項1に記載の 方法。 4. 前記染色工程が蛍光化合物をウィルスと結合することを含む請求項1に記載 の方法。 5. 前記染色工程が蛍光化合物を血小板と結合することを含む請求項1に記載の 方法。 6. 前記染色工程が蛍光化合物を細菌と結合することを含む請求項1に記載の方 法。 7. 前記染色工程が蛍光化合物を原虫類と結合することを含む請求項1に記載の 方法。 8. 前記染色工程が蛍光化合物を寄生虫と結合することを含む請求項1に記載の 方法。 9. 前記染色工程が蛍光化合物をDNA分子と結合することを含む請求項1に記載 の方法。 10.前記染色工程が蛍光化合物をRNA分子と結合することを含む請求項1に記載 の方法。 11.前記染色工程を前記試薬添加工程の後で行う請求項1に記載の方法。 12.前記試薬添加工程が単独の希釈物を全血液サンプルへ添加することを含む請 求項1に記載の方法。 13.前記試薬添加工程が等張性生理食塩溶液を全血液サンプルへ添加することを 含む請求項1に記載の方法。 14.前記試薬添加工程が高張性溶液を全血液サンプルへ添加することを含む請求 項1に記載の方法。 15.前記試薬添加工程が試薬をサンプルのpHを変える全血液サンプルへ添加する ことを含む請求項1に記載の方法。 16.前記試薬添加工程が、清浄剤を全血液サンプルへ添加することを含む請求項 1に記載の方法。 17.前記試薬添加工程が、アルキル両性イオン化合物を全血液サンプルへ添加す ることを含む請求項1に記載の方法。 18.前記試薬添加工程が、式n-オクチル-N,N-ジメチル-3-アミノ-1-プロパ ンスルホネートを有する化合物を全血液サンプルへ添加することを含む請求項 1に記載の方法。 19.前記試薬添加工程が、実質的に全血液サンプルの最初の容積に影響を与えな い乾燥試薬を全血液サンプルへ添加することを含む請求項1に記載の方法。 20.前記蛍光化合物励起工程が、550nmより大きい波長を有するレーザー光を提 供することを含む請求項1に記載の方法。 21.前記蛍光化合物励起工程が、550nmより大きい発光波長を有する蛍光化合物 を提供することを含む請求項1に記載の方法。 22.前記蛍光化合物励起工程が、650-700nmの範囲の発光波長を有する蛍光化 合物を提供することを含む請求項1に記載の方法。 23.全血液サンプルを得; 試薬を全血液サンプルへ添加して全血液サンプルにおける赤血球が凝集する ことを阻止し; 蛍光化合物の定量的量を全血液サンプルへ添加して混合物を作り; 蛍光化合物および全血液サンプルを、蛍光化合物が全血液サンプルの標的成 分と結合するようにインキュベーションし; 混合物の一部を一定容積の室へ入れ、ここにおいて混合物は実質的に均一な ヘマトクリット層を提供する 工程を有する蛍光画像形成装置による分析のための全血液の静的サンプルを調 製する方法。 24.さらに、標的成分から発光する蛍光が混合物の走査カラムのバックグラウン ド蛍光の少なくとも1/4であるような強度を有する光の励起ビームを提供する 工程を含む請求項23の方法。 25.前記光の励起ビームを提供する工程が、550nmより大きい波長を有するレー ザー光を提供することを含む請求項24の方法。 26.前記光の励起ビームを提供する工程が、600-650nmの範囲にピーク波長を 有するレーザー光を提供することを含む請求項24の方法。 27.さらに、標的成分から発光する蛍光が混合物の走査カラムのバックグラウン ド蛍光と実質的に同じであるような強度を有する光の励起ビームを提供する工 程を含む請求項23の方法。 28.前記蛍光化合物および全血液をインキュベーションする工程を、前記蛍光化 合物の定量的量を添加する工程の後で行う請求項23の方法。 29.前記混合物の一部を室へ入れる工程が、5-60μの範囲のヘマトクリット層を 提供することを含む請求項23の方法。 30.前記混合物の一部を室へ入れることが、10-200μの範囲の深さの走査毛細管 を提供することを含む請求項23の方法。 31.前記混合物の一部を室へ入れることが、100μの深さの走査毛細管を提供す ることを含む請求項23の方法。 32.前記蛍光化合物の定量的量を添加する工程が、500nmより大きい発光波長を 有する蛍光化合物を提供することを含む請求項23の方法。 33.前記蛍光化合物の定量的量を添加する工程が、650-700nmの範囲にピーク 波長を有する蛍光化合物を提供することを含む請求項23の方法。 34.前記蛍光化合物の定量的量を添加する工程が、モノクロナール抗体と結合す るシアニン染料を提供することを含む請求項23の方法。 35.さらに、公知数の微粒子を全血液サンプルへ添加して室における混合物の容 積を測定する工程を含む請求項23の方法。 36.全血液サンプルを提供し; 全血液サンプルを、全血液サンプルの少なくとも二つの標的成分と選択的に 結合するように形成した少なくとも二つの蛍光化合物で染色し; 試薬を全血液サンプルへ添加して全血液サンプルにおいて赤血球が凝集する ことを阻止し; 赤血球が実質的に標的成分の分類を妨害しない前記励起波長を有する光で蛍 光化合物を励起する 工程を有する静的血液サンプルにおける標的成分を分類する分析方法。 37.室; 全血液サンプル; 赤血球の凝集を阻止する試薬; 蛍光複合体 を含み、その際、前記全血液サンプル、前記試薬および前記蛍光複合体が前記 室に配置されて実質的に均一なヘマトクリット層を形成するものであるサンプ ル調製物。 38.前記室が10-200μの範囲の深さを有する走査毛細管である請求項37のサンプ ル調製物。 39.前記室が、100μの深さを有する走査毛細管である請求項37のサンプル調製 物。 40.前記蛍光複合体が、全血液サンプル内に含まれる白血球に発現された抗原に 対し特異的である抗体と共有結合した蛍光染料を含む請求項37のサンプル調製 物。 41.ヘマトクリット層が、5ないし60μの範囲の深さを有する請求項37のサンプ ル調製物。 42.前記希釈剤が等張性生理食塩溶液である請求項37のサンプル調製物。 43.前記試薬が清浄剤である請求項37のサンプル調製物。 44.前記試薬がアルキル両性イオン性化合物である請求項37のサンプル調製物。 45.前記試薬が式n-オクチル-N,N-ジメチル-3-アミノ-1-プロパンスルホネ ートを有する請求項37のサンプル調製物。
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US08/328,241 US5585246A (en) | 1993-02-17 | 1994-10-24 | Method for preparing a sample in a scan capillary for immunofluorescent interrogation |
US08/328,241 | 1994-10-24 | ||
PCT/US1995/012305 WO1996012963A1 (en) | 1994-10-24 | 1995-09-22 | Method for preparing a sample in a scan capillary for immunofluorescent interrogation |
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JP2000510572A true JP2000510572A (ja) | 2000-08-15 |
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JP51391796A Expired - Lifetime JP3623513B2 (ja) | 1994-10-24 | 1995-09-22 | 免疫蛍光法による調査のための走査毛細管におけるサンプルの調製方法 |
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US (2) | US5585246A (ja) |
EP (1) | EP0788604B1 (ja) |
JP (1) | JP3623513B2 (ja) |
AT (1) | ATE241140T1 (ja) |
AU (1) | AU704473B2 (ja) |
CA (1) | CA2203098A1 (ja) |
DE (1) | DE69530855T2 (ja) |
WO (1) | WO1996012963A1 (ja) |
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1994
- 1994-10-24 US US08/328,241 patent/US5585246A/en not_active Expired - Lifetime
-
1995
- 1995-09-22 AT AT95935134T patent/ATE241140T1/de not_active IP Right Cessation
- 1995-09-22 CA CA002203098A patent/CA2203098A1/en not_active Abandoned
- 1995-09-22 AU AU37268/95A patent/AU704473B2/en not_active Ceased
- 1995-09-22 WO PCT/US1995/012305 patent/WO1996012963A1/en active IP Right Grant
- 1995-09-22 DE DE69530855T patent/DE69530855T2/de not_active Expired - Lifetime
- 1995-09-22 JP JP51391796A patent/JP3623513B2/ja not_active Expired - Lifetime
- 1995-09-22 EP EP95935134A patent/EP0788604B1/en not_active Expired - Lifetime
-
1996
- 1996-10-28 US US08/739,131 patent/US5932428A/en not_active Expired - Lifetime
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006518462A (ja) * | 2003-02-20 | 2006-08-10 | クオリジエン・インコーポレイテツド | 診断装置および方法 |
JP4738325B2 (ja) * | 2003-02-20 | 2011-08-03 | クオリジエン・インコーポレイテツド | 診断装置および方法 |
JP2008537105A (ja) * | 2005-03-24 | 2008-09-11 | ユニヴァーシティ コート オブ ザ ユニヴァーシティ オブ エディンバラ | 抗原検出 |
JP4834075B2 (ja) * | 2005-03-24 | 2011-12-07 | ザ ユニヴァーシティ コート オブ ザ ユニヴァーシティ オブ エディンバラ | 抗原検出 |
JP2009527734A (ja) * | 2006-03-28 | 2009-07-30 | ヘモク アクチボラゲット | 蛍光標識生物学的成分の検出のための装置及び方法 |
Also Published As
Publication number | Publication date |
---|---|
ATE241140T1 (de) | 2003-06-15 |
EP0788604A1 (en) | 1997-08-13 |
WO1996012963A1 (en) | 1996-05-02 |
US5585246A (en) | 1996-12-17 |
DE69530855T2 (de) | 2004-02-26 |
AU704473B2 (en) | 1999-04-22 |
EP0788604A4 (en) | 1998-12-30 |
DE69530855D1 (de) | 2003-06-26 |
CA2203098A1 (en) | 1996-05-02 |
AU3726895A (en) | 1996-05-15 |
JP3623513B2 (ja) | 2005-02-23 |
US5932428A (en) | 1999-08-03 |
EP0788604B1 (en) | 2003-05-21 |
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