JP2000500584A - 網状赤血球を鑑別および同定するための試薬系および方法 - Google Patents
網状赤血球を鑑別および同定するための試薬系および方法Info
- Publication number
- JP2000500584A JP2000500584A JP9519822A JP51982297A JP2000500584A JP 2000500584 A JP2000500584 A JP 2000500584A JP 9519822 A JP9519822 A JP 9519822A JP 51982297 A JP51982297 A JP 51982297A JP 2000500584 A JP2000500584 A JP 2000500584A
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- reticulocyte
- sample
- potassium
- reticulocytes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 網状赤血球染色用試薬を含み、この網状赤血球染色用試薬はアズールB、 ニュー・メチレン・ブルー、ブリリアント・クレシル・ブルーおよびオキサジン 750よりなる群から選択される約0.7〜約1.3gの核酸染料とn−ドデシ ルβ−D−マルトシド、N−ドデシル−N,N−ジメチル−3−アンモニオ−1 −プロパンスルホネート(DDAPS)およびN−テトラデシル−N,N−ジメ チル−3−アンモニオ−1−プロパンスルホネート(TDAPS)よりなる群か ら選択される約3〜約7mgの網状赤血球球状化剤とシュウ酸のアンモニウム塩 、カリウム塩およびナトリウム塩よりなる群から選択される約17〜約23gの 電解質とを含み;染色用試薬濃度は希釈剤試薬1L当たりであり;希釈剤試薬は 約2.33〜約2.57gの燐酸ナトリウム(二塩基性)と約0.38〜0.4 2gの燐酸カリウム(一塩基性)と約0.18〜約0.22gのEDTA二ナト リウム(二水塩)と約7.25〜約8.86gの塩化ナトリウムと約0.36〜 約0.44gの塩化カリウムと約0.28〜約0.35gのプロクリン300と を含み;さらに脱イオン水 にて1Lとなし、希釈剤試薬は約7.2〜約7.6のpHを有すると共に550 mOs/kgより大の浸透圧モル濃度(osmolarity)を有することを特徴とす る網状赤血球を測定するための試薬系。 2. 網状赤血球染色用試薬が約19.0〜約21.0gのシュウ酸カリウム( 一水塩)と約0.95〜約1.05gのニュー・メチレン・ブルーと約4.75 〜約5.25mgのn−ドデシルβ−D−マルトシドとを含む請求の範囲第1項 に記載の試薬系。 3. 網状赤血球染色用試薬が約20.0gのシュウ酸カリウム(一水塩)と約 1.0gのニュー・メチレン・ブルーと約5.0mgのn−ドデシルβ−D−マ ルトシドとを含む請求の範囲第2項に記載の試薬系。 4. 網状赤血球染色用試薬が約1.0Lの希釈剤試薬をさらに含む請求の範囲 第3項に記載の試薬系。 5. 希釈剤試薬が約2.33〜約2.57gの燐酸ナトリウム(二塩基性)と 約0.38〜約0.42gの燐酸カリウム(一塩基性)と約0.19〜約0.2 1gのEDTA二ナトリウム(二水塩)と約7.65〜約8.45gの塩化ナト リウム と約0.38〜約0.42gの塩化カリウムと約0.30〜約0.33gのプロ クリン300とを含む請求の範囲第1項に記載の試薬系。 6. 希釈剤試薬が約2.45gの燐酸ナトリウム(二塩基性)と約0.40g の燐酸カリウム(一塩基性)と約0.20gのEDTA二ナトリウム(二水塩) と約8.05gの塩化ナトリウムと約0.40gの塩化カリウムと約0.315 gのプロクリン300とを含む請求の範囲第5項に記載の試薬系。 7. a. 血液試料の1部を網状赤血球試薬系と混合し、網状赤血球試薬系は アズールB、ニュー・メチレン・ブルー、ブリリアント・クレシル・ブルーおよ びオキサジン750よりなる群から選択される約0.7〜約1.3gの核酸染料 とn−ドデシルβ−D−マルトシド、N−ドデシル−N,N−ジメチル−3−ア ンモニオ−1−プロパンスルホネート(DDAPS)およびN−テトラデシル− N,N−ジメチル−3−アンモニオ−1−プロパンスルホネート(TDAPS) よりなる群から選択される約3〜約7mgの網状赤血球球状化剤とシュウ酸のア ンモニウム塩、カリウム塩およびナトリウム塩よりなる群から選択される約17 〜約23gの電解質と約1Lの希釈剤試薬と を含み、希釈剤試薬は約2.33〜約2.57gの燐酸ナトリウム(二塩基性) と約0.38〜約0.42gの燐酸カリウム(一塩基性)と約0.19〜約0. 21gのEDTA二ナトリウム(二水塩)と約7.65〜約8.45gの塩化ナ トリウムと約0.38〜約0.42gの塩化カリウムと約0.30〜約0.33 gのプロクリン300とを含み; b. 試料/試薬の混合物を約15分間〜約4時間にわたり保温し; c. 試料/試薬の混合物を一度に実質的に1個の細胞にて集中光学照明の領域 に通過させ、この通過に際し試料/試薬の混合物を流れとして流動させると共に 、試料/試薬の流れをさらに低張性シース試薬の流動する流れ内にさらに包被さ せ; d. 試料/試薬の流れにおける細胞により散乱された光を検出すると共に、そ こからデータを発生させ; e. 発生した散乱光データを集め; f. 試料/試薬の流れにおける他の細胞から網状赤血球を少なくとも部分的に 収集散乱光データに基づき鑑別する ことを特徴とする血液試料における網状赤血球の鑑別および定量方法。 8. 散乱光を約0°、10°および90°の角度で検出する請求の範囲第7項 に記載の方法。 9. 網状赤血球の鑑別が少なくとも部分的に10°および90°の散乱光に基 づく請求の範囲第7項に記載の方法。 10. 血小板凝血物および合併イベントを、収集された10°の散乱光データ により排除する請求の範囲第9項に記載の方法。 11. 約3.7〜約4.3mLの網状赤血球試薬系を約10〜約40μLの凝 固防止された全血と混合する請求の範囲第7項に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/560,601 | 1995-11-20 | ||
US08/560,601 US5733784A (en) | 1995-11-20 | 1995-11-20 | Reagent system and method for the differentiation and identification of reticulocytes |
PCT/US1996/018471 WO1997019356A1 (en) | 1995-11-20 | 1996-11-18 | Reagent system and method for the differentiation and identification of reticulocytes |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000500584A true JP2000500584A (ja) | 2000-01-18 |
JP3725553B2 JP3725553B2 (ja) | 2005-12-14 |
Family
ID=24238497
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51982297A Expired - Fee Related JP3725553B2 (ja) | 1995-11-20 | 1996-11-18 | 網状赤血球を鑑別および同定するための試薬系および方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US5733784A (ja) |
EP (1) | EP0864091B1 (ja) |
JP (1) | JP3725553B2 (ja) |
AT (1) | ATE249630T1 (ja) |
CA (1) | CA2237473C (ja) |
DE (1) | DE69629938T2 (ja) |
ES (1) | ES2206613T3 (ja) |
WO (1) | WO1997019356A1 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014517324A (ja) * | 2011-06-17 | 2014-07-17 | コンスティテューション・メディカル・インコーポレイテッド | 生体試料の組織処理のための溶液 |
JP2015522165A (ja) * | 2012-07-05 | 2015-08-03 | ベックマン コールター, インコーポレイテッド | 白血球数の測定方法及び測定装置 |
WO2024095648A1 (ja) * | 2022-11-04 | 2024-05-10 | 株式会社堀場製作所 | 測定方法、測定システムおよび検査用試薬キット |
Families Citing this family (19)
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FR2759166B1 (fr) * | 1997-01-31 | 1999-04-23 | Abx Sa | Reactif de coloration pour la determination de cellules sanguines |
US5935857A (en) * | 1997-08-01 | 1999-08-10 | Coulter International Corp. | Blood diluent |
US5874311A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of reticulocytes in blood |
US6060322A (en) * | 1998-10-20 | 2000-05-09 | Coulter International Corp. | Method for identification of reticulated cells |
US6271035B1 (en) * | 1998-10-20 | 2001-08-07 | Coulter International Corp. | Methods and compositions for rapid staining of nucleic acids in whole cells |
JP3886271B2 (ja) * | 1998-11-27 | 2007-02-28 | シスメックス株式会社 | 赤芽球の分類計数用試薬及び分類計数方法 |
US6444471B1 (en) | 1999-10-18 | 2002-09-03 | Research & Diagnostic Systems, Inc. | Reticulocyte containing complete blood control |
US6784981B1 (en) * | 2000-06-02 | 2004-08-31 | Idexx Laboratories, Inc. | Flow cytometry-based hematology system |
US6706526B2 (en) * | 2002-01-24 | 2004-03-16 | Coulter International Corp. | Low formaldehyde producing blood diluent |
CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
US8102161B2 (en) * | 2007-09-25 | 2012-01-24 | Tdk Corporation | Stable output in a switching power supply by smoothing the output of the secondary coil |
CN101475754A (zh) * | 2008-01-04 | 2009-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料,组合物及在生物样品染色中的用途 |
CN101602762B (zh) * | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
CN101726579B (zh) * | 2008-10-17 | 2014-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液检测试剂和方法 |
US8367358B2 (en) * | 2008-12-17 | 2013-02-05 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Reagent, kit and method for differentiating and counting leukocytes |
CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
CA3211350A1 (en) | 2018-03-30 | 2019-10-03 | Idexx Laboratories, Inc. | Flow cytometer, laser optics assembly thereof, and methods of assembling the same |
WO2019206297A1 (zh) * | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | 一种血液分析仪及分析方法 |
KR102547788B1 (ko) | 2020-06-17 | 2023-06-26 | 아이덱스 래보러토리즈, 인코포레이티드 | 플로우 사이토미터 및 그 레이저 광학 어셈블리 |
Family Cites Families (10)
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US5045472A (en) * | 1981-06-26 | 1991-09-03 | Technicon Instruments Corporation | Reagent mixture and composition for treating red blood cells to effect sphering |
US4412004A (en) * | 1981-06-26 | 1983-10-25 | Technicon Instruments Corporation | Method for treating red blood cells to effect sphering and reagent therefor |
US4575490A (en) * | 1984-02-29 | 1986-03-11 | Technicon Instruments Corporation | One step method for sphering and fixing whole blood erythrocytes |
US5075556A (en) * | 1989-12-01 | 1991-12-24 | Technicon Instruments Corporation | Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood |
US5360739A (en) * | 1991-12-05 | 1994-11-01 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
US5350695A (en) * | 1991-12-05 | 1994-09-27 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
US5284771A (en) * | 1991-12-05 | 1994-02-08 | Miles Inc. | Reagent compositions and their use in sphering cells |
DE69432410T2 (de) * | 1993-05-14 | 2004-03-04 | Coulter International Corp., Miami | Retikulozyt bestimmungsverfahren und geraet, das lichtstreuungstechniken verwendet |
US5487975A (en) * | 1993-11-15 | 1996-01-30 | Ventana Medical Systems, Inc. | Biotin/avidin formulation |
US5691204A (en) * | 1995-04-21 | 1997-11-25 | Abbott Laboratories | Compositions and methods for the rapid analysis of reticulocytes |
-
1995
- 1995-11-20 US US08/560,601 patent/US5733784A/en not_active Expired - Lifetime
-
1996
- 1996-11-18 ES ES96942767T patent/ES2206613T3/es not_active Expired - Lifetime
- 1996-11-18 JP JP51982297A patent/JP3725553B2/ja not_active Expired - Fee Related
- 1996-11-18 AT AT96942767T patent/ATE249630T1/de not_active IP Right Cessation
- 1996-11-18 CA CA002237473A patent/CA2237473C/en not_active Expired - Fee Related
- 1996-11-18 DE DE69629938T patent/DE69629938T2/de not_active Expired - Lifetime
- 1996-11-18 EP EP96942767A patent/EP0864091B1/en not_active Expired - Lifetime
- 1996-11-18 WO PCT/US1996/018471 patent/WO1997019356A1/en active IP Right Grant
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014517324A (ja) * | 2011-06-17 | 2014-07-17 | コンスティテューション・メディカル・インコーポレイテッド | 生体試料の組織処理のための溶液 |
JP2015522165A (ja) * | 2012-07-05 | 2015-08-03 | ベックマン コールター, インコーポレイテッド | 白血球数の測定方法及び測定装置 |
WO2024095648A1 (ja) * | 2022-11-04 | 2024-05-10 | 株式会社堀場製作所 | 測定方法、測定システムおよび検査用試薬キット |
Also Published As
Publication number | Publication date |
---|---|
ES2206613T3 (es) | 2004-05-16 |
CA2237473A1 (en) | 1997-05-29 |
WO1997019356A1 (en) | 1997-05-29 |
EP0864091A1 (en) | 1998-09-16 |
DE69629938D1 (de) | 2003-10-16 |
JP3725553B2 (ja) | 2005-12-14 |
DE69629938T2 (de) | 2004-07-15 |
US5733784A (en) | 1998-03-31 |
CA2237473C (en) | 2004-11-02 |
EP0864091B1 (en) | 2003-09-10 |
ATE249630T1 (de) | 2003-09-15 |
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