JP2000325467A - Blood component sampling apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood component sampling apparatus which can decrease amt. of removal of blood during thrombocyte sampling work and little generates side effects on blood sampling such as bad feeling during the thrombocyte sampling work. SOLUTION: A blood component sampling apparatus is provided with a centrifugal separator driving device, a pump 11, a pump 12 and a control part 13 for controlling a plurality of flow path opening/closing means in such a way that a thrombocyte sampling work comprising the first plasma sampling step for sampling plasma, a const. speed plasma circulation step wherein the plasma in a plasma sampling bag 25 is circulated into a centrifugal separator 20, an accelerated speed plasma circulation step wherein the plasma in the plasma sampling bag 25 is circulated into a centrifugal separator 20 while it is accelerated, and a blood returning step, is perfomed. The control part controls in such a way that the thrombocyte sampling work is performed for a plurality of times and, in the blood returning step in the thrombocyte sampling works before the last time, the plasma is returned until wt. of the plasma sampled before it is decreased to the necessary lowest remaining plasma vol.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component collecting apparatus for separating a predetermined blood component from blood.

[0002]

2. Description of the Related Art At the time of blood collection, currently, blood is separated into each blood component by centrifugation or the like, for reasons such as effective use of blood and reduction of the burden on donors, and only components necessary for the transfuser are collected. Samples are collected and other components are collected and returned to donors. In such a component blood sampling, when a platelet preparation is obtained, blood collected from a donor is introduced into a blood component collection circuit, and a plasma, a centrifugal bowl called a centrifugal bowl installed in the blood component collection circuit is used.
The blood is separated into four components, white blood cells, platelets, and red blood cells, and the platelets are collected in a container to produce a platelet product, and the remaining plasma, white blood cells, and red blood cells are returned to the donor.

As a method for collecting platelets, for example, Japanese Patent Application Laid-Open
There is a method disclosed in JP-A-509403. Japanese Patent Publication No. Hei 8-509403 discloses a first method in which a liquid is added to whole blood at a predetermined flow rate and diluted and sent to a centrifugal bowl. Whole blood is sent to a centrifugal bowl, and centrifuged to separate low-density components. After separating the medium density component and the high density component into the first container and taking out the low density component into the first container, the circuit is switched and the low density component is circulated at the first flow rate (constant speed) so as to be circulated in the centrifugal bowl. A second method is disclosed in which a density component region is expanded and a medium density component is extracted while recirculating at a second flow rate (acceleration).

[0004]

However, in the above-mentioned first method, three pumps, an ACD pump, a blood collection pump and a circulation pump, are required, and the apparatus becomes large. Also,
In the second method, platelets can be collected by two pumps, and the size of the apparatus can be reduced. However, leukocytes are frequently mixed in the collected platelets. As a platelet-containing liquid for a platelet preparation, a liquid containing less leukocytes is required. In addition, in the conventional method, plasma is collected together with platelets, and no special device has been devised for the method of collecting plasma. Usually platelet collection
A certain amount of platelets is collected by repeating the platelet collection process consisting of one cycle a plurality of times. In the plasma collection performed in parallel, a system is adopted in which a plasma amount obtained by equally dividing the target plasma collection amount by the number of cycles to be performed is collected, and the remainder is returned.

[0005] The plasma accumulated in the plasma collection bag is also a part of the blood taken out of the body, and is a part of the amount of blood removed from the donor. This is not a problem if the donor is large and has a large total blood volume.However, in order to collect platelets from a larger number of donors, even if the person is not large, there is no problem with other conditions. We donate blood. In the case of such a blood donor, it is preferable that the blood removal amount during the platelet collection operation is reduced as much as possible so as not to cause blood collection side effects such as bad mood during the platelet collection operation.

Accordingly, an object of the present invention is to reduce the size of the apparatus, to reduce the amount of white blood cells mixed therein, to increase the efficiency of platelet collection, to obtain a high-concentration platelet-containing liquid, and to perform a plurality of platelet collection operations. Blood component collection device that collects plasma together with platelets by performing blood collection, blood components during platelet collection work increase little, and blood components that do not cause side effects such as poor mood during platelet collection work A sampling device is provided.

[0007]

Means for achieving the above object include a rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and rotating the rotor to rotate the inlet. A centrifugal separator for centrifuging the introduced blood in the blood storage space, a first line for connecting a blood collection needle or a blood collection device connection to an inlet of the centrifuge, and the centrifuge. A second line connected to the outlet, a third line connected to the first line for anticoagulant injection, and a first tube connected in the middle of the first line A blood component collection device comprising a blood component collection circuit comprising a plasma collection bag having a second tube connected to the second line and a platelet collection bag connected to the second line, The blood A centrifugal separator driving device for rotating the rotor of the centrifugal separator, and a centrifugal separator disposed closer to the centrifugal separator side than a connection between the first line and the first tube; A first liquid feed pump for the third line, a second liquid feed pump for the third line, and a plurality of flow path opening / closing means for opening and closing the flow path of the blood component collection circuit. A control unit for controlling the centrifugal separator driving device, the first liquid feed pump, the second liquid feed pump, and the plurality of flow path opening / closing means, wherein the control unit includes an anticoagulant. Collecting the added blood, separating the collected blood and collecting the separated plasma in the plasma collection bag, and centrifuging the plasma in the plasma collection bag collected by the plasma collection step. Plasma circulation to circulate through the separator Tsu consists of a flop,
After the plasma collection / circulation step performed at least once, and after the plasma collection / circulation step, the plasma circulation speed is accelerated to cause the platelets to flow out of the centrifuge and collect the platelets in the platelet collection bag. The platelet collection step, after completion of the platelet collection step, such that the platelet collection operation to perform a blood return step of returning the blood in the centrifuge is performed a plurality of times, the centrifuge drive device,
The first liquid feed pump, the second liquid feed pump and the plurality of flow path opening / closing means are controlled.
In the blood return step in the platelet collection operation before the last round, the blood component collection is controlled so that the plasma is collected until the weight of the plasma collected before the blood return step reaches a predetermined required residual plasma volume. Device.

In order to achieve the above object, there is provided a rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and is introduced from the inlet by rotation of the rotor. A centrifuge for centrifuging blood in the blood storage space, a first line for connecting a blood collection needle or a blood collection device connection to an inlet of the centrifuge, and the outlet of the centrifuge A second line connected to the first line, a third line for anticoagulant injection connected to the first line, a first tube connected in the middle of the first line, and the second line. A blood component collection device comprising a blood component collection circuit comprising a plasma collection bag having a second tube connected to a line and a platelet collection bag connected to the second line,
The blood component collection device, a centrifugal separator driving device for rotating the rotor of the centrifugal separator, and disposed on the centrifuge side from the connection between the first line and the first tube, A first liquid supply pump for a first line, a second liquid supply pump for the third line, and a plurality of flow paths for opening and closing the flow path of the blood component collection circuit Means, a control unit for controlling the centrifugal separator driving device, the first liquid feed pump, the second liquid feed pump and the plurality of flow path opening / closing means, the control unit comprises:
Collection of blood to which an anticoagulant has been added, separation of the collected blood and collection of the separated plasma in the plasma collection bag, and plasma collection in the plasma collection bag collected by the plasma collection step. A plasma circulating step of circulating plasma through the centrifuge, wherein at least 1
Plasma collection / circulation step to be performed twice, and after the plasma collection / circulation step, a platelet collection step of accelerating the plasma circulation speed, causing platelets to flow out of the centrifuge, and collecting platelets in the platelet collection bag. After the platelet collection step, the centrifugal separator driving device and the first transporter are configured to perform a platelet collection operation for performing a blood return step of returning blood in the centrifuge a plurality of times. And controlling the liquid pump, the second liquid sending pump, and the plurality of flow path opening / closing means. Further, in the blood return step in the platelet collection operation before the final round, the plasma collected before the blood return step It is a blood component collection device that controls the return of plasma so that the weight falls within the range of the residual plasma volume (y) under the following conditions.

(Conditions) The residual plasma volume (y) is not less than B when A is equal to or more than A and A ≦ B. Volume of plasma collected at the end of platelet collection operation before blood step)
/ (Total number of platelet collection operations -1)

The control section controls the plasma collection and circulation step to be performed twice.
In the first plasma collection / circulation step, a plasma collection / constant speed circulation step of circulating the plasma in the plasma collection bag at a constant speed to the centrifuge is performed, and a second plasma collection / circulation step is performed.
In the circulating step, it is preferable that control is performed such that a plasma collection / accelerated circulation step of circulating the plasma in the plasma collection bag while accelerating the plasma in the centrifugal separator is performed.

Further, in the blood return step in the final platelet collection operation, the control unit controls the plasma collected in the plasma collection bag not to be returned by the final blood return step. Preferably, there is. Further, the control unit, in the blood return step in the final platelet collection operation, if the weight of the plasma collected by the final blood return step exceeds the target plasma collection amount,
It is preferable to control so that excess plasma is returned. Further, the predetermined required residual plasma volume is 1
It is preferably 0 to 50 ml. Further, it is preferable that the control unit has a function of controlling a centrifugal rotation speed of a rotor of the centrifuge according to a hematocrit value of a blood donor. Further, the blood component collection device includes a hematocrit value input unit or a hematocrit value measurement function, and the control unit uses the input or measured hematocrit value to calculate a rotor rotation speed suitable for the hematocrit value. It is preferable to have a rotation speed calculation function and a function of controlling the centrifugal separator driving device using the rotor rotation speed calculated by the rotor rotation speed calculation function.

[0012] It is preferable that the blood component collection device includes a weight sensor for the plasma collection bag. Further, the control unit, such that the platelet collection operation is performed at least twice, the centrifuge drive device,
It is preferable to control the first liquid feed pump, the second liquid feed pump, and the plurality of flow path opening / closing means. Further, the blood component collection circuit includes a buffy coat collection bag connected to the second line, the control unit is after the platelet collection step,
Prior to the blood return step, the plasma circulation speed of the first liquid feeding pump is set higher than the final speed in the platelet collection step, and the buffy coat is discharged from the centrifuge to remove the buffy coat from the buffy coat collection bag. It is preferable to perform a buffy coat collecting step of collecting the buffy coat.

Further, the blood component collecting circuit is provided with the second component.
And the control unit controls the plasma circulation speed by the first liquid supply pump after the platelet collection step and before the blood return step. A buffy coat collecting step of causing the buffy coat to flow out of the centrifuge and collecting the buffy coat into the buffy coat collecting bag while making the same or higher than the final speed in the step, and reducing the rotation speed of the rotor of the centrifuge. Is preferably performed. Further, when the platelet collection operation is further performed after the completion of the buffy coat collection step, the control unit returns the collected buffy coat to the centrifuge before the next blood collection step. It is preferable that the first liquid feed pump and the plurality of flow path opening / closing means are controlled so as to perform a step.

[0014]

DESCRIPTION OF THE PREFERRED EMBODIMENTS A blood component collecting apparatus according to the present invention will be described with reference to an embodiment shown in the drawings. FIG. 1 is a plan view showing a configuration example of a blood component collection circuit used in the blood component collection device of the present invention. FIG. 2 is a plan view of a cassette housing portion of the blood component collection circuit of FIG. FIG. 3 is a partially cutaway sectional view showing a state in which a driving device is mounted on a centrifuge used in a blood component collection circuit, and FIG. It is a conceptual diagram of one Example of an apparatus. The blood component collection device 1 of the present invention includes a rotor 142 having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and stores blood introduced from the inlet by rotation of the rotor 142 into the blood storage space. A centrifugal separator 20 for centrifuging the blood, a first line 21 for connecting a connection to a blood collection needle 29 or a blood pool and an inlet of the centrifuge 20, and a centrifuge 20.
A second line 22 connected to the outlet of the first tube 21; a third line 23 connected to the first line 21 for anticoagulant injection; and a first tube 2 connected to the first line 21.
5a and a plasma collection bag 25 having a second tube 25b connected to the second line 22;
2 is a blood component collection device for the blood component collection circuit 2 including a platelet collection bag 26 connected to the blood sample collection circuit 2.

The blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20 and a first liquid supply pump 1 for the first line 21.
1 and the second pump 12 for the third line 23
And a plurality of flow path opening / closing means 81, 82, 83, 84, 85, 86, for opening and closing the flow path of the blood component collection circuit 2.
87, the centrifuge drive device 10, the first liquid feed pump 1
A control unit 13 for controlling the first and second liquid feed pumps 12 and a plurality of flow path opening / closing means is provided.

Therefore, the blood component collecting circuit 2 will be described first. The blood component collection circuit 2 is a circuit for collecting blood components, especially platelets. The platelet collection circuit 2 includes a blood collection device such as a blood collection needle 29, a connection portion to a blood collection device having a blood collection needle or a blood pool connection portion (blood collection device connection portion), a blood collection needle 29 or a blood collection device connection portion, and a centrifuge. The first line 21 (blood collection and blood return line), which is connected to the inlet of the first pump tube 20 and the first pump tube 21g, the outlet of the centrifuge 20 and the first line 21
, The first line 2 and the second line 22 for connecting
A third line 23 (an anticoagulant injection line) which is connected near the first blood collection needle 29 and includes a second pump tube 23a, and a branch connector located closer to the blood collection needle than the pump tube 21g of the first line 21 A plasma collection bag 25 having a first tube 25a connected to 21f and a second tube 25b connected to the second line 22;
The platelet collection bag 26 including the third tube 26a connected to the second line 22, the buffy coat collection bag 27 including the fourth tube 27a connected to the second line 22, the liquid connected to the second line 22 ( It has a fourth line 24 for injection of saline. The blood component collection circuit 2 may include a connection unit (for example, a metal or synthetic resin needle) for connecting to a blood pool such as a blood bag instead of a blood collection needle.

As the blood collection needle 29, a known metal needle is used. The first line 21 includes a first line 21 a on the blood collection needle side to which the blood collection needle 29 is connected and a first line 21 b on the centrifuge side connected to the inlet of the centrifuge 20.
The blood collection needle side first line 21a is formed by connecting a plurality of soft resin tubes. Blood collection needle side first line 21
a is a branch connector 21c for connection to the third line 23, a chamber 21d for removing bubbles and microaggregates, a branch connector 21e for connection to the second line 22, and a plasma collection bag 25 from the blood collection needle side. First
A branch connector 21f for connection to the tube 25a is provided. An air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d. The centrifuge-side first line 21b is connected to a branch connector 21f for connection to the first tube 25a, and has a pump tube 21g formed in the vicinity thereof.

The outlet of the centrifuge 20 and the first line 2
One end of the second line 22 connecting the first line 21 is connected to the outlet of the centrifuge 20, and the other end is connected to the connection branch connector 21 e of the first line 21. The second line 22 is a branch connector 22a for connection with the second tube 25b of the plasma collection bag 25 and the third tube 26a of the platelet collection bag 26, and a connection branch for connection with the fourth line 24 from the centrifuge side. The baffle coat collecting bag 27 includes a connector 22b, a branch connector 22c for connection to a tube including a bubble removal filter 22f, and a branch connector 22d for connection to a fourth tube 27a of the buffy coat collection bag 27.

One end of the third line 23 is connected to a connection branch connector 21c provided on the first line 21. The third line 23 is connected to the pump tube 23a, the foreign matter removing filter 23 from the connector 21c side.
b, a bubble removing chamber 23c and an anticoagulant container connecting needle 23d. One end of the fourth line 24 is connected to the connection branch connector 22 b of the second line 22. The fourth line 24 includes a foreign matter removing filter 24a and a saline container connecting needle 24b from the connector 22b side.

The plasma collection bag 25 is connected to the first line 21
The first tube 25a and the second tube 25a connected to the branch connector 21f located closer to the blood collection needle than the pump tube 21g.
Connected to the branch connector 22a of the line 22 of FIG.
It has a tube 25b. The platelet collection bag 26 includes a third tube 26a connected to the branch connector 22a of the second line 22. The buffy coat collection bag 27 includes a fourth tube 27a connected to the branch connector 22d of the second line 22.

The above-mentioned first to fourth lines 21 and 22
The constituent materials of the tubes used for forming the tubes 2, 23, 24, the pump tubes, and the tubes connected to the bag include, for example, polyvinyl chloride, polyethylene, polypropylene, polyesters such as PET and PBT, and ethylene-ethylene. Vinyl acetate copolymer, polyurethane,
Examples thereof include thermoplastic elastomers such as polyester elastomers and styrene-butadiene-styrene copolymers. Among them, polyvinyl chloride is particularly preferable. If each tube is made of polyvinyl chloride, sufficient flexibility,
Because flexibility is obtained, it is easy to handle, and it is also suitable for clogging by cleansing or the like. Also, as the constituent material of the branch connector described above, the same material as the constituent material of the tube can be used. As the pump tube, a tube having such a strength as not to be damaged even when pressed by a roller pump is used.

Plasma collection bag 25, platelet collection bag 2
6. The buffy coat collection bag 27 is formed by laminating a resin-made flexible sheet material and fusing (thermal fusion, high-frequency fusion, or the like) or bonding the periphery thereof to form a bag. used. As a material used for each of the bags 25, 26, 27, for example, soft polyvinyl chloride is preferably used. As a plasticizer in this soft polyvinyl chloride, for example, di (ethylhexyl) phthalate (D
EHP), di- (n-decyl) phthalate (DnDP)
Etc. are used. In addition, the content of such a plasticizer,
The amount is preferably about 30 to 70 parts by weight based on 100 parts by weight of polyvinyl chloride.

As the sheet material of each of the bags 25, 26, and 27, a polyolefin, that is, a polymer obtained by polymerizing or copolymerizing an olefin such as ethylene, propylene, butadiene, or isoprene or a diolefin may be used. Specifically, for example, polyethylene,
Polypropylene, ethylene-vinyl acetate copolymer (EV
A), a polymer blend of EVA and various thermoplastic elastomers, or a combination thereof arbitrarily. Furthermore, polyethylene terephthalate (PET), polybutylene terephthalate (PBT),
Polyesters such as poly-1,4-cyclohexanedimethyl terephthalate (PCHT) and polyvinylidene chloride can also be used.

As the sheet material used for the platelet collection bag 26, it is more preferable to use a sheet material having excellent gas permeability in order to improve platelet preservation. As such a sheet material, for example, using the above-described polyolefin or DnDP plasticized polyvinyl chloride,
Further, without using such a material, a sheet material of the above-described material is used, and the thickness is relatively thin (for example,
Those having a thickness of about 0.1 to 0.5 mm, particularly about 0.1 to 0.3 mm) are preferable. Further, the platelet collection bag includes, for example, physiological saline, GAC, PAS, PSM-1.
Such a platelet preservation solution as described above may be stored in advance.

The main part of the blood component collecting circuit 2 is of a cassette type as shown in FIG. The blood component collection circuit 2 includes all lines (first line, second line, third line, and fourth line) and all tubes (first tube, second tube, third tube, and fourth line). Tubes) are partially housed and partially hold them, in other words comprise a cassette housing 28 in which they are partially fixed. Both ends of a first pump tube 21g and both ends of a second pump tube 23a are fixed to the cassette housing 28. The pump tubes 21g and 23a are formed into a loop shape corresponding to the shape of the roller pump by the cassette housing 28. It is protruding. Therefore, the first and second pump tubes 21g and 23a can be easily mounted on the roller pump.

Further, the cassette housing 28 has a plurality of openings located in the cassette housing 28. More specifically, the first opening portion 91 exposing the first line 21 on the blood collection needle side from the pump tube 21g and allowing the first flow path opening / closing means 81 of the blood component collection device 1 to enter, First tube 25a of plasma collection bag 25
And the second opening 92 through which the second flow path opening / closing means 82 of the blood component collection device 1 can enter, the second tube 25b of the plasma collection bag 25 is exposed, and the second The third in which the third channel opening / closing means 83 can enter.
Opening 93, the fourth tube 94 exposing the third tube of the platelet collection bag 26 and allowing the fourth flow path opening / closing means 84 of the blood component collection device 1 to enter, the second line 2
2 and the fourth tube 27 of the buffy coat collection bag 27
a of the position on the centrifugal separator side (upstream side) from the connection with
Of the blood component collection device 1
A fifth opening 95 through which the flow path opening / closing means 85 can enter;
A second portion between the connection portion with the first line 21 and the connection portion with the fourth tube 27a of the buffy coat collection bag 27 (downstream from the connection portion between the second line 22 and the fourth tube 27a). The sixth opening 96 that allows the line 22 to be exposed and allows the sixth flow path opening / closing means 86 of the blood component collection device 1 to enter, the fourth line 24 to be exposed, and the seventh component of the blood component collection device 1 to be exposed. The seventh in which the flow path opening / closing means 87 can enter.
The opening 97 is provided.

On the inner surface of the cassette housing 28, the above-described branch connector is fixed. Further, near the side surface of the cassette housing 28, a line and a tube protruding from the side surface of the housing are held,
Further, a reinforcing tube for preventing bending at the housing portion is provided. Cassette housing 28
Is a box-shaped body in which the portion shown by the broken line in FIG. 2 can be stored. Then, the cassette housing 28
Is formed of a synthetic resin having a certain degree of rigidity.

The blood component collecting apparatus 1 includes the cassette housing mounting portion (not shown). For this reason, by mounting the cassette housing 28 on the cassette housing mounting portion of the blood component collecting apparatus 1, each line and each tube of the portion exposed from the opening of the cassette housing 28 automatically correspond to the corresponding channel opening / closing means. Attached to. Thus, the circuit can be easily mounted, and the preparation for collecting blood components can be quickly performed. In addition, the blood component collection device 1 is provided with two pumps near the cassette housing mounting portion. Therefore, the pump tube exposed from the cassette housing 28 can be easily mounted on the pump.

The centrifugal separator 20 provided in the blood component collection circuit 2 is usually called a centrifuge bowl, and separates blood components by centrifugal force. As shown in FIG. 3, the centrifugal separator 20 has a vertically extending tube 141 having an inlet 143 formed at the upper end, and rotates around the tube 141, and is liquid-tightly sealed to the upper portion 145. Hollow rotor 14
And 2. A flow path (blood storage space) is formed in the rotor 142 along the bottom and the inner surface of the peripheral wall,
An outlet 144 is formed so as to communicate with an upper part of the flow path. In this case, the volume of the rotor 142 is, for example, about 100 to 350 ml.

The rotor 142 is rotated under predetermined centrifugal conditions (rotation speed and rotation time) set by the rotor rotation drive device 10 provided in the blood component collection device 1. According to the centrifugation conditions, a blood separation pattern (for example, the number of blood components to be separated) in the rotor 142 can be set. In the present embodiment, as shown in FIG.
The centrifugation conditions are set so that the separation is performed into the buffy coat layer 132 and the red blood cell layer 133.

Next, the blood component collecting apparatus 1 of the present invention shown in FIG. 4 will be described. The blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid supply pump 11 for the first line 21, and a third line 23. And a plurality of flow path opening / closing means 81, 82, 83, 84, 8 for opening and closing the flow path of the blood component collection circuit 2.
5, 86, 87, and a control unit 13 for controlling the centrifugal separator driving device 10, the first liquid feed pump 11, the second liquid feed pump 12, and the plurality of flow path opening / closing means. Further, the blood component collection device 1 is mounted above the turbidity sensor 14 and the centrifuge 20 which are mounted on the second line 22 on the centrifuge side (upstream side) from the connection portion 22a with the second tube 25b. And a weight sensor 16 for detecting the weight of the plasma collection bag 25. Furthermore, the control unit 13 may have a function of controlling the centrifugal rotation speed of the rotor of the centrifuge according to the blood component concentration of the donor. The blood component concentration of the donor may be, for example, a hematocrit value or a platelet concentration, or may be an expected number of collected platelets calculated from the hematocrit value and the platelet concentration.

In this embodiment, the blood component sampling device has a hematocrit value input unit. The control unit 13 includes two pump controllers (not shown) for the first liquid supply pump 11 and the second liquid supply pump 12, and includes a control mechanism of the control unit 13 and the first liquid supply pump 11 and The second liquid feed pump 12 is electrically connected via a pump controller, and is also electrically connected to a drive controller included in the centrifuge drive device (rotor drive device) 10. The control unit 13 uses a hematocrit value input unit, a rotor speed calculation function for calculating a rotor speed suitable for the hematocrit value using the input hematocrit value, and a rotor speed calculated by the rotor speed calculation function. It has the function of controlling the heart separator driving device using the numbers.

The channel opening / closing means 81, 82, 83, 8
4, 85, 86, and 87 are all connected to the control unit 13, and their opening and closing are controlled by the control unit 13.
Further, a turbidity sensor 14, an optical sensor 15 mounted above the centrifuge 20, and a weight sensor 16 for detecting the weight of the plasma collection bag 25 are also electrically connected to the control unit 13, and The output signal is the control unit 1
3 is input. The control unit 13 has a control mechanism composed of, for example, a microcomputer and a function of calculating the number of rotations of the rotor.
5. The detection signal from the turbidity sensor 14 is input to the control unit 13 as needed. The control unit 13 controls the rotation, stop, and rotation direction (forward / reverse) of each pump based on signals from the turbidity sensor 14, the optical sensor 15, and the weight sensor 16 and, if necessary, controls each flow. It controls the opening and closing of the road opening and closing means and the operation (rotation of the rotor) of the centrifugal separator rotation drive device 10. The control unit 13 has, as a rotor rotation speed calculation function, a hematocrit value and a rotor rotation speed proper relational expression data storage unit, and an input hematocrit value and a stored hematocrit value and a rotor rotation speed proper relational expression data. It has a function to calculate the proper rotor speed. The appropriate relational data between the hematocrit value and the rotor speed are data obtained by actually collecting platelets at various rotor speeds using various hematocrit values of blood and obtaining good results (hematocrit value and rotor speed). ) Can be calculated. The relational expression can be expressed as a first-order, second-order, or even higher-order regression equation. The hematocrit value obtained by the present inventors (x:
%) And an appropriate relational expression data of the rotor rotation speed (y: rpm) are as follows. ^{y = 40x + 3000 y = -0.94505x} 2 + 110.18x + 1744 y = 0.0667x 3 -8.7619x 2 +391.9
x-1277.4 In any of the examples of the appropriate relational expression data, the tendency is that the lower the hematocrit value, the lower the proper rotor speed, and conversely, the higher the hematocrit value, the higher the proper rotor speed.

When the hematocrit value (x) is input, a proper rotor speed (y) is calculated from the stored data of the proper relational expression between the hematocrit value and the rotor speed. The rotor rotation speed calculated by the rotor rotation speed calculation function when necessary (at the time of plasma collection step described later, at the time of plasma collection / constant speed circulation step, at the time of the second plasma collection step, and at the time of plasma collection / acceleration circulation step). Controls the centrifuge drive to rotate the rotor. By changing the rotor speed in accordance with the hematocrit value in this way, for example, in the case of a blood donor with a low hematocrit value,
It is possible to prevent excessive compression and reduction of the separated blood component layer due to excessive centrifugation called packing. When such packing occurs, the platelet layer is also compressed, so that platelet collection is difficult and collection efficiency is reduced. Also, conversely,
In the case of a blood donor having a high hematocrit value, it is possible to prevent an insufficient separation state due to insufficient centrifugation. When such a poorly separated state occurs, many platelets not located in the platelet layer are present in the blood cell layer or plasma,
The sampling efficiency is reduced. However, in the apparatus of the present invention, the above-mentioned reduction in packing and the occurrence of insufficient separation are extremely reduced, and platelets can be collected efficiently.

The first channel opening / closing means 81 is provided for opening / closing the first line 21 on the blood collection needle side from the pump tube 21g. Second channel opening / closing means 82
Is provided for opening and closing the first tube 25a of the plasma collection bag 25. The third channel opening / closing means 83 includes:
It is provided for opening and closing the second tube 25b of the plasma collection bag 25. The fourth channel opening / closing means 84 is provided for opening / closing the third tube 26 a of the platelet collection bag 26. The fifth flow path opening / closing means 85 is connected to the second line 22 at a position closer to the centrifugal separator (upstream side) than the connection 22 d between the second line 22 and the fourth tube 27 a of the buffy coat collection bag 27. It is provided for opening and closing. The sixth flow passage opening / closing means 86 is provided between the connection portion 21e with the first line 21 and the connection portion with the fourth tube 27a (downstream from the connection portion between the second line 22 and the fourth tube 27a). ) Is provided for opening and closing the second line 22. Seventh channel opening / closing means 87
Is provided for opening and closing the fourth line 24. The channel opening / closing means includes a line or tube insertion portion, and the insertion portion has a clamp operated by a drive source such as a solenoid, an electric motor, and a cylinder (hydraulic or pneumatic). Specifically, an electromagnetic clamp operated by a solenoid is preferable. The clamp of the flow path opening / closing means operates based on a signal from the control unit 13.

As shown in FIG. 3, the rotor driving device 10 holds a rotor rotation driving device housing 151 accommodating the centrifugal separator 20, a leg 152, a motor 153 as a driving source, and the centrifugal separator 20. Disc-shaped fixed base 1
55. The housing 151 includes the leg 1
It is placed and fixed on the upper part of the reference numeral 52. Further, a spacer 15 is provided on the lower surface of the housing 151 by a bolt 156.
7, the motor 153 is fixed. Motor 15
A fixed base 155 is fitted to the tip of the third rotating shaft 154 so as to rotate coaxially and integrally with the rotating shaft 154, and the bottom of the rotor 142 is fitted to the upper part of the fixed base 155. A recess is formed. In addition, centrifuge 2
0 is fixed to the housing 151 by a fixing member (not shown). Rotor rotation drive 10
Then, when the motor 153 is driven, the fixed base 155 and the rotor 142 fixed thereto are rotated, for example, at a rotation speed of three.
Spin at 000-6000 rpm.

Further, the rotor rotation driving device housing 1
The inner wall of the centrifuge 51 has an interface between the separated blood components (for example, the plasma layer 131 and the buffy coat layer 132).
B, buffy coat layer 132 and erythrocyte layer 133
Optical sensor 15 for optically detecting the position of
Are installed and fixed by a mounting member 158. As the optical sensor 15, an optical sensor that can scan vertically along the outer peripheral surface of the rotor 142 is used. This sensor includes a light source that irradiates light toward the shoulder portion of the centrifuge 20 and a light receiving unit that receives light that is reflected back from the centrifuge bowl. That is, LE
A light-emitting element such as D or a laser and a light-receiving element are arranged in a row, and light reflected from a blood component of light emitted from the light-emitting element is received by the light-receiving element, and the amount of received light is photoelectrically converted. Have been. Since the intensity of the reflected light differs depending on the separated blood components (for example, the plasma layer 131 and the buffy coat layer 132), the position corresponding to the light receiving element whose received light amount has changed is detected as the position of the interface B.
More specifically, the amount of light received by the light receiving unit when the light passing through the centrifuge 20 is filled with a transparent liquid (plasma or water) and when the light is filled with a buffy coat layer It is detected from the difference that the buffy coat layer has reached the light passing portion. The position at which the buffy coat layer is detected is adjusted by changing the position at which light passes through the bowl. Usually, once the light passing position is determined, it is fixed there.

The turbidity sensor 14 is for detecting the turbidity of the fluid flowing in the second line 22, and outputs a voltage value corresponding to the turbidity. Specifically, a low voltage value is output when the turbidity is high, and a high voltage value is output when the turbidity is low.

Pump tube 21g of first line 21
Non-blood contact type pumps such as a roller pump and a peristaltic pump are preferable as the first liquid supply pump 11 to which the pump is mounted and the second liquid supply pump 12 to which the pump tube 23a of the third line 23 is mounted. It is. Also,
As the first liquid sending pump 11 (blood pump), a pump capable of sending blood in any direction is used.
Specifically, a roller pump capable of normal rotation and reverse rotation is used.

The control section collects the blood to which the anticoagulant has been added, separates the collected blood, and collects the separated plasma in the plasma collection bag. And a plasma circulating step of circulating the plasma in the plasma collection bag through the centrifugal separator. The plasma collecting and circulating step is performed at least once. Specifically, the control unit 13 operates the first liquid supply pump 11 and the second liquid supply pump 12 to collect blood to which the anticoagulant has been added, and the centrifugal separator driving device 1
Activate 0 (with the above calculated value or set value,
Rotating the rotor) to collect plasma from blood 25
A first plasma collection step of collecting a first predetermined amount of plasma, and then temporarily suspending the blood collection and operating the centrifuge driving device 10 (the above-described calculated value or setting). At a constant value), and a constant-speed plasma circulation step (constant-speed circulation) for circulating the plasma in the plasma collection bag to the centrifuge 20 at a constant speed (constant-speed circulation). First), and then
The first liquid feed pump 11 and the second liquid feed pump 12 are operated to collect blood to which the anticoagulant is added, and the centrifuge driving device 10 is operated (to the above-described calculated value or set value). And rotate the rotor) until the interface sensor detects a predetermined position (for example, a buffy coat layer).
A second plasma collection step of collecting plasma, and after completion of the second plasma collection step, suspending blood collection, and
An accelerated plasma circulating step in which the centrifugal separator driving device 10 is operated (by rotating the rotor with the above-mentioned calculated value or set value) to accelerate and circulate the plasma in the plasma collection bag 25 to the centrifugal separator 20 (Accelerated circulation), a plasma collection / accelerated circulation step (second time) comprising: and after the plasma collection / accelerated circulation step, the plasma circulation speed by the first liquid supply pump 11 is accelerated,
A platelet collecting step of causing platelets to flow out of the centrifuge 20 and collecting the platelets into a platelet collection bag, and a blood return step of returning blood in the centrifuge 20 after the platelet collecting step is completed. is there. In the second plasma collection step, the weight of the plasma bag is not detected because the detection is performed by the interface sensor.

Further, the blood component collecting apparatus 1 of this embodiment
The control unit 13 performs centrifugation so that the platelet collection operation including the plasma collection / constant-speed circulation step, the plasma collection / accelerated circulation step, the platelet collection step, and the blood return step is performed a plurality of times (at least twice). Device driving device 10, first liquid supply pump 11, second liquid supply pump 12
And a plurality of flow path opening / closing means. Furthermore, in the blood return step in the platelet collection operation before the last round, the control unit returns the blood, which is the blood cell component in the centrifugal separator, and the weight of the plasma collected before the blood return step to a predetermined required minimum. The plasma is returned until the plasma volume (first predetermined value) is reached. Then, in the blood return step in the final platelet collection operation, the first liquid supply pump 11 and the plurality of blood pumps are collected so that the plasma collected by the final blood return step is collected in the plasma collection bag 25. The flow path opening / closing means is controlled. The required minimum residual plasma volume refers to a volume of plasma that can fill a tube portion of a circuit necessary for plasma circulation, and varies depending on the circuit, but is about 10 to 50 ml. This can be easily confirmed by previously measuring the volume of the portion of the platelet collection circuit (blood component collection circuit) used in the plasma circulation.

In this way, at the end of each platelet collection operation before the last round, only the required minimum amount of collected plasma is returned, and the rest is returned, so that each platelet collection operation is completed by the returned plasma volume. At that time (in other words, at the start of the next platelet collection operation), the blood removal amount becomes smaller by that amount, and the burden on the donor is reduced. Also,
Only in the final round, by collecting the plasma collected in the plasma collection bag 25 up to the final blood return step, it is possible to collect plasma together with platelets,
It can be effectively used as a raw material for plasma preparations.

Further, in the blood return step in the platelet collection operation before the last round, the control unit returns the weight of the plasma collected before the blood return step together with the blood return (the plasma collection bag 25).
Is returned so that the plasma weight within the range falls within the range of the residual plasma volume (y) under the following conditions. Furthermore, in the blood return step in the final platelet collection operation, the weight of the plasma collected by the collection of the target plasma collection amount into the plasma collection bag 25 or the final blood return step reaches the target plasma collection amount. Otherwise, if the collected plasma is collected in the plasma collection bag 25 and the weight of the collected plasma by the final blood return step exceeds the target plasma collection amount, the excess plasma is returned. The first liquid feed pump 11 and the plurality of flow path opening / closing means are controlled so that the above operation is performed. (Conditions) Residual plasma volume (y) is not less than A and not more than B when A ≦ B A = predetermined required residual plasma volume (first predetermined value) B = number of platelet collection operations × (target plasma collection volume− Volume of plasma collected at the end of the first platelet collection operation) / (total number of platelet collection operations-1)

By controlling in this way, it is possible to collect the target plasma at or near the target plasma collection volume, and the plasma collection volume before the final platelet collection operation is equal to that under normal conditions. As compared with the method of collecting blood, the amount of blood removed before the last round is also reduced, and the burden on the donor can be reduced. For example, when the control of the control unit is performed as in this embodiment, when the target plasma collection volume is 300 ml and the platelet collection operation is performed five times (the required minimum remaining plasma volume A is 50 ml), the first plasma collection is performed. 20 samples
If it is 0 ml, the above B is 1 · (300-2
00) / (5-1) = 25, and because A> B, the residual plasma volume was 50, and in the blood return step, 200-50
= Return 150 ml of plasma. In the second platelet collection operation, B is 2 · (300-200) / (5-
1) = 50, and in the blood return step, the plasma weight was 50
ml of plasma (in other words, almost all plasma collected by the second platelet collection operation is returned. In the third platelet collection operation, the above B
Is 3 · (300−200) / (5-1) = 75, and in the blood return step, returns the plasma until the plasma weight reaches 75 ml (in other words, the plasma collected by the third platelet collection operation) Do not return only the 25 ml inside). In the fourth platelet collection operation, B is 4 ·
(300-200) / (5-1) = 100, and in the blood return step, the plasma is returned until the plasma weight reaches 100 ml (in other words, only 25 ml of the plasma collected by the fourth platelet collection operation) Will not be returned). In the final platelet collection operation, the plasma is not substantially returned. For this reason, since there is almost no change in the condition from the first time, about 2
Since 00 ml of plasma is collected, the target 300 ml of plasma can be collected by combining this with the amount of retained plasma up to the fourth time.

Further, the blood component collecting apparatus 1 of this embodiment
The control unit 13 after the end of the platelet collection step,
Before the blood return step, the plasma circulation speed by the first liquid feeding pump 11 is set higher than the final speed in the platelet collection step, the buffy coat is discharged from the centrifuge 20 and the buffy coat is collected in the buffy coat collection bag 27. The buffy coat collection step is controlled to be performed. The buffy coat collecting step is not limited to the above-described method. For example, the plasma circulation speed by the first liquid feeding pump 11 is maintained at the final speed in the platelet collecting step, and This may be performed by lowering the rotation speed of the rotor. Further, the buffy coat collecting step may be performed by making the plasma circulation speed by the first liquid sending pump higher than the final speed in the platelet collecting step and lowering the rotation speed of the rotor of the centrifuge.

After the buffy coat collection step is completed, a first buffy coat return step for returning the collected buffy coat into the centrifuge 20 before the next blood collection step is performed. 11 and a plurality of flow path opening / closing means. Specifically, an anticoagulant is added to whole blood at a predetermined ratio (1/8 to 1/20, specifically 1/10 with respect to whole blood), and a predetermined speed (250 ml /
min or less; preferably, 150 to 40 ml / min or less, specifically, 60 ml / min or less), sent to the centrifuge 20 via the first line 21,
Calculated or set value (3000 to 600
0 rpm) to turn the blood into plasma, buffy coat,
Separated into each component of red blood cells, and when the plasma overflows the centrifuge 20, the blood is collected in a plasma bag, and when a predetermined amount of plasma (10 to 150 ml, preferably 20 to 30 ml) has been collected, the blood transmission is stopped. The plasma is subjected to a predetermined condition (at a speed higher than the blood collection volume, 60 to 250 ml / min for 10 to 90 seconds, specifically, the first circulation is 200 ml).
/ Min × 30 sec) and return to the centrifugal separator 20 through the first line 21 and the second line 22 to perform constant-speed plasma circulation.

Then, an anticoagulant is again added to whole blood (1/8 to 1/20, specifically 1/10 with respect to whole blood).
At a predetermined rate (250 ml / min or less; preferably, 150 to 40 ml / min or less, specifically, 60 ml / min or less) to the centrifugal separator 20 via the first line 21 for centrifugation. The rotor of the centrifuge 20 is rotated at a calculated value or a set value (3000 to 6000 rpm) to separate blood into components of plasma, buffy coat, and red blood cells. Stop sending blood at the time of detection,
The plasma was subjected to predetermined conditions (initial speed 60 to 80 ml / min, final arrival speed (set speed) 150 to 250 ml / min, acceleration conditions (every second) at a speed increase of 2 to 10 ml / min,
At a circulation time of 10 to 90 sec), the accelerated plasma circulation is performed by returning to the centrifuge 20 through the first line 21 and the second line 22.

Then, under predetermined conditions (blood collection amount: 0 to 2500)
/ Hct% [ml], specifically, 400 to 1000 /
Hct% [ml]), the anticoagulant-supplemented whole blood was again sent, and the plasma was separated under the predetermined conditions into the first and second lines 21 and 21.
22 and return to the centrifugal separator 20 and increase the acceleration stepwise under predetermined conditions (stepwise acceleration;
1 to 99 ml / min / sec, specifically, 2 to 10
ml / min / sec) Platelet collection speed (60 to 250
ml / min; actually, 200 ml / min), and collects platelets flowing out of the centrifuge 20 into the platelet collection bag 26. Furthermore, in this device, after collecting platelets, the blood circulation speed is maintained (60 to 25).
0 ml / min, specifically 200 ml / min)
And reducing the rotation speed of the centrifugal separator 20 (by lowering the rotation speed by about 100 to 300 rpm, preferably at 4500 to 4600 rpm).
The buffy coat that has flowed out is collected, and the collected buffy coat is supplied to the centrifugal separator 20 before blood collection in the next cycle is performed. In addition, the collection of the buffy coat, after collecting the platelets, the blood circulation rate to a predetermined speed (more than the platelet collection rate, preferably 60 to 250 ml
/ Min, specifically 205 ml / min).

FIG. 4 shows a blood component collecting step (first platelet collecting operation) by the blood component collecting apparatus 1 of this embodiment.
This will be described with reference to the flowcharts of FIGS.
In this embodiment, the platelet collection operation is repeated three times, and after the end of the platelet collection step other than the last one, before the blood return step, the buffy coat collection step is performed, and before the next blood collection step, Centrifuge 20
A buffy coat return step for returning this is performed.

(Example 1) First, the third line 23 and the blood collection needle 29 are primed with an anticoagulant, and then the donor is punctured with a puncture needle. Then, a small amount of blood is collected, a hematocrit value is measured by a hematocrit measuring device prepared separately, and the value is input from a hematocrit value input section as shown in FIG. The control unit calculates an appropriate rotor speed from the input hematocrit value. The first liquid feed pump 11 and the second liquid feed pump 12 are operated to collect the blood to which the anticoagulant has been added, and the centrifugal separator driving device 10 rotates the rotor at the calculated value to convert the blood into blood plasma. A first plasma collection step of collecting a first predetermined amount of plasma in the collection bag 25 is performed.

When the first blood collection is started, the blood pump 1
1 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is also driven at a predetermined speed (for example, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.
Blood collected from the donor is mixed with ACD fluid and
, Passes through the chamber and the first channel opening / closing means 81, and flows into the centrifuge 20. At this time,
Sixth flow path opening / closing means 86, fifth flow path opening / closing means 85, second flow path opening / closing means 82, third flow path opening / closing means 83, seventh flow path
Are closed, and the first channel opening / closing means 81 and the fourth channel opening / closing means 84 are open. When the ACD blood is supplied to the centrifuge 20, the centrifuge 20
The sterile air that has entered flows through the second line 22 and
, And flows into the platelet collection bag 26. The rotor of the centrifugal separator 20 starts rotating at the calculated value (for example, 4800 rpm) at the same time as the start of the blood collection process, and the centrifugal separator 20 receives the supply of ACD blood while rotating. Is done,
Blood is applied from the inside to the plasma layer and buffy coat layer (BC
Layer) and red blood cell layer, and when ACD blood (about 270 ml) exceeding the capacity of the separator is supplied, the inside of the centrifuge 20 is completely filled with blood, and the outlet of the centrifuge 20 Out of the plasma. The turbidity sensor 14 attached to the second line 22 connected to the outlet of the centrifuge 20 detects that the fluid flowing in the line has changed from air to plasma, and the control unit 13 Based on the detection signal of the turbidity sensor 14, the fourth flow path opening / closing means 84 is closed and the third flow path opening / closing means 83 is opened, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. For this reason, the weight of the plasma collected in the plasma collection bag 25 is the first predetermined amount (10 to 150 g, for example, 20 g).
When the number increases, the control unit 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a constant speed circulation step.

In the constant-speed circulation step, the blood collection is temporarily stopped, and the centrifuge drive device 10 is operated to maintain the rotation of the rotor of the centrifuge 20 at the calculated value, and the blood is collected in the plasma collection bag 25. The plasma thus obtained is circulated through the centrifuge 20 at a constant speed. When entering the constant-speed circulation step, the control unit 13 sets the closed state of the first flow path opening / closing unit 81 and the second state.
, The second liquid feed pump (ACD pump) 12 is stopped, and the first liquid feed pump (blood pump) 11 is driven at a predetermined speed (60 to 250 ml / m).
In, for example, 200 ml / min), the plasma in the plasma collection bag 25 is sent to the rotating centrifuge 20 at a calculated value (for example, 4800 rpm) through the second flow path opening / closing means 82. At the same time, the plasma flowing out of the centrifuge 20 flows into the plasma collection bag 25 through the turbidity sensor 14 and the third channel opening / closing means 83. After the constant-speed circulation step starts, a predetermined time (10 to 90 seconds, for example,
After elapse of 30 seconds), the control unit 13 closes the second flow path opening / closing means 82, opens the first flow path opening / closing means 81, and shifts to the second plasma collection step. The first plasma circulation is
It is preferable to carry out at a flow rate of at least 60 ml / min or more for 10 seconds or more.

In the second plasma collection step, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added, and the amount of plasma in the bag is increased. When the optical sensor 15 detects the buffy coat layer of the separator, this signal is sent to the control unit 13, and the control unit 13 closes the first flow path opening / closing means 81 and opens and closes the second flow path The means 82 is opened to move to the accelerated plasma circulation step. Specifically, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min).
At this time, the second pump, which is an anticoagulant pump, simultaneously supplies the anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). Blood collected from the donor is mixed with the ACD solution, flows into the centrifugal separator 20 where the rotor rotates at a calculated value (for example, 4800 rpm), and plasma is collected in the plasma collection bag 25. Normally, when the optical sensor 15 detects the buffy coat layer of the separator due to an increase in the amount of plasma in the bag, this signal is sent to the control unit 13, and the control unit 13 transmits the signal to the first channel opening / closing unit 81. Is closed, the second flow path opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. In the plasma collection step, the sensor is buffy coat (BC interface:
Plasma is collected until the detection of the interface between the plasma layer and the buffy coat layer). In the apparatus of this embodiment, as shown in the flowcharts of FIGS. 5, 7, and 9, the detection of the BC interface is performed in each of the first to last plasma collection steps, If a BC interface is detected during these first to final plasma collection steps, the plasma collection is interrupted and a transition is made to the accelerated plasma circulation step.

In the accelerated circulation step, the blood collection is temporarily stopped, and the centrifuge drive device 10 is operated to maintain the rotation of the rotor of the centrifuge 20 at the calculated value, and the plasma in the plasma collection bag 25 is removed. Circulation is accelerated through the centrifuge 20. At this time, the blood pump speed is lower than the constant speed circulation step, for example, starts at 60 ml / min, and accelerates until the final speed reaches 200 ml / min. The acceleration condition is 6.7 m per second.
It is performed with a 200 ml / min arrival time of about 21 seconds at which the 1 / min speed increases. After the end of this circulation step, the process proceeds to FIG. 6, and a blood collection step for interface adjustment is performed.

As shown in FIG. 6, the blood sampling step for adjusting the interface can be said to be a small-quantity plasma sampling step. In this step, in order to keep the position of the buffy coat layer constant in the subsequent platelet collection step regardless of the donor, blood is collected by a predetermined supply amount of red blood cells. The red blood cell supply amount is defined as a value obtained by dividing the blood collection amount by the hematocrit value of the donor, and the blood collection amount is generally about 12 ml. Also in this blood collection, the first liquid supply pump 11 operates at a predetermined speed (for example, 60 m).
1 / min). At this time, the second pump, which is an anticoagulant pump, also simultaneously transmits the anticoagulant (for example, ACD) at a predetermined speed (for example, 1/10 of the blood pump speed).
-A solution). Blood collected from the donor was AC
It is mixed with the solution D, flows into the centrifugal separator 20 that rotates at a calculated value (for example, 4800 rpm), and a small amount of plasma is collected. The control unit 13 calculates the collection time from the set collection amount and the pump speed, and ends the blood collection when the collection time has elapsed. Then, the control unit 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a platelet collection step.

After the above steps are completed, the plasma circulation speed of the first liquid feed pump 11 is increased, and the centrifuge 20
Platelets are drained from the inside and platelets are collected in a platelet collection bag 26.
A platelet collection step of collecting platelets. The platelet collection step is also called a so-called acceleration process. In this step, the blood pump speed is, in this example, 60 ml /
The control unit 13 operates the blood pump so as to accelerate the blood pump from 10 min / min to 200 ml / min every predetermined time (for example, 1 second) by 10 ml / min.
Once in is reached, the speed is maintained until the platelet collection step is completed.

When the platelet collection step is started, the turbidity sensor 14 detects the turbidity of the liquid passing therethrough, and the turbidity is output as a voltage value by the sensor.
3 is input. When the speed of the blood pump increases and reaches approximately 160 to 200 ml / min, platelets contained in the buffy coat layer remaining in the centrifuge 20 flow out. When the platelets flow out, the turbidity of the liquid passing through the turbidity sensor 14 increases, and when the voltage value output from the sensor drops by 0.2 V, the third flow path opening / closing means 83 closes and the fourth flow The path opening / closing means 84 is opened, and platelet-rich plasma flowing out of the centrifuge 20 is collected in the platelet collection bag 26. The voltage value output from the turbidity sensor 14 is converted into a platelet concentration by the control unit 13, and the platelet concentration in the platelet collection bag 26 during platelet collection is calculated. The platelet concentration in the platelet collection bag 26 once reaches the maximum concentration and then decreases. At the point in time when it is detected that the maximum concentration has been reached, the platelet collection step ends, and the process proceeds to the buffy coat collection step.

In the buffy coat collecting step, when the above-described platelet collecting step is completed, the control unit 13
Is closed, and the fifth channel opening / closing means 85 is opened. The blood plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 by the blood pump 11, and the liquid (the buffy coat layer has flowed out) from the centrifuge 20 simultaneously flows into the buffy coat collection bag 27. .
In the buffy coat collection step, the blood pump speed is maintained at the final speed in the platelet collection step. At the time when the amount calculated from the hematocrit value of the toner and the amount of collected platelets is collected, the blood pump 11 is stopped, all valves are closed, the rotation of the rotor of the centrifuge 20 is stopped, and the buffy coat collection step is completed. .

Next, a blood return step (blood return step) for returning the blood in the centrifuge 20 is performed. In the blood return step,
It moves to FIG. In this embodiment, the centrifuge 20
Plasma return is performed together with the return of blood cell components inside. Therefore,
The blood return step is a step of performing both blood cell component return and plasma return. First, the flow path opening / closing means 82, the third flow path opening / closing means 83, the fourth flow path opening / closing means 84, the fifth flow path opening / closing means 85, the sixth flow path opening / closing means 86, and the seventh flow path The opening / closing means 87 is closed, only the first flow path opening / closing means 81 is opened, and the blood pump 11 rotates in the reverse direction. As a result, the blood cell components in the centrifuge 20 pass through the tubes 21b and 21a and are returned to the donor via the blood collection needle 29. And this blood cell component return is shown in FIG.
As shown in the figure, the blood return volume is one predetermined blood return volume (for example, 5
0 ml). The flow rate is measured by the number of revolutions of the pump. Subsequently, plasma return is performed. In this plasma return, the plasma is not directly returned to the blood donor, but is made to flow into the centrifuge 20. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. In the plasma return, it is checked whether the plasma weight (PPP) in the plasma collection bag 25 is equal to or more than a predetermined necessary residual plasma amount (secured ppp, for example, 50 g). Determine if the return set plasma volume (eg, 50 g) has been reached,
When it reaches, the plasma weight (PPP) in the plasma collection bag 25 again becomes the predetermined required residual plasma amount (securing ppp,
For example, it is checked whether it is 50 g) or more.
The return of plasma is stopped and the blood cell component is returned again. Then, the above steps are repeated until the plasma collection bag 2
When the plasma weight (PPP) in the sample 5 has reached a predetermined required residual plasma volume (securing ppp, for example, 50 g), it is determined whether or not the blood cell component return is completed (specifically, attached to the tube 21b). Sensor 31 (for example,
An ultrasonic sensor, an optical sensor or an infrared sensor) detects whether there is a blood component in the tube). Then, when the presence of the blood component in the tube is confirmed, the blood cell component return is started, and it is finally confirmed that the blood cell component return end state has been reached, and the blood return step ends. As a result, the red blood cell layer remaining in the centrifugal separator 20 and the plasma having a predetermined residual plasma amount or more are returned to the donor. Thus, the first (first) platelet collection operation is completed.

Subsequently, the operation proceeds to the second platelet collection operation shown in FIG. First, as shown in FIG. 8, a buffy coat return step of returning the buffy coat collected in the first platelet collection step into the centrifuge 20 before the next blood collection step is performed. In the buffy coat return step, the control unit 13 rotates the rotor of the centrifuge 20 with the calculated value to open the fifth flow path opening / closing means 85 and the fourth flow path opening / closing means 84, and sets the blood pump. 11 is a predetermined speed (default is 100ml / min)
Operate with The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of the buffy coat collected, the buffy coat return step ends.

After the above-described first plasma collection step, constant-speed circulation step, second plasma collection step, and accelerated circulation step, the process proceeds to FIG. , A buffy coat collection step and a blood return step are sequentially performed. In the blood return step, the process proceeds to FIG. 10, and the blood return step is performed in the same manner as the first time, and the second platelet collection operation is completed.

Next, the final platelet collection operation shown in FIGS. 11 to 13 will be described. In this embodiment, the third time is the final time, but the present invention is not limited to this.
The fourth and subsequent times may be the final platelet collection operation. In this case, except for the last time, the operation is the same as the second platelet collection operation (FIGS. 7 and 8). First, as shown in FIG. 11, the buffy coat collected by the second platelet collection operation was centrifuged before the next blood collection step.
Perform the buffy coat return step to return within. When shifting to the buffy coat return step, the control unit 13
By rotating the rotor of the centrifuge 20 with the calculated value, the fifth
And the fourth flow path opening / closing means 84 is opened, and the blood pump 11 is operated at a predetermined speed (the default is 100 m).
1 / min). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of buffy coat collected,
The buffy coat return step ends.

Next, the rotation of the rotor of the centrifugal separator 20 at the calculated value is maintained, and the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added. Then, a first plasma collection step of collecting a first predetermined amount of plasma from the blood into the plasma collection bag 25 is performed.

When the blood collection is started, the first liquid feeding pump 1
1 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is also driven at a predetermined speed (for example, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.
Blood collected from the donor is mixed with ACD fluid and
, Passes through the chamber and the first channel opening / closing means 81, and flows into the centrifuge 20. At this time,
Sixth flow path opening / closing means 86, fifth flow path opening / closing means 85, second flow path opening / closing means 82, third flow path opening / closing means 83, seventh flow path
Is closed, the first channel opening / closing means 81 and the fifth channel opening / closing means 85 are open, and when the ACD blood is supplied to the centrifuge 20, the centrifugal separation is performed. The sterilized air contained in the vessel 20 flows into the buffy coat collection bag 27 through the line sensor and the fifth flow path opening / closing means 85. Simultaneously with the start of the blood collection process, the centrifuge 20 keeps rotating at a predetermined speed (for example, 4800 rpm), and the centrifugal separator 20 receives the supply of ACD blood while rotating. The blood is separated from the inside into a plasma layer, a buffy coat layer (BC layer), and an erythrocyte layer. The inside is completely filled with blood, and plasma flows out from the outlet of the centrifuge 20. Turbidity sensor 14 attached to a second line 22 connected to the outlet of centrifuge 20
Detects that the fluid flowing in the line has changed from air to plasma, and based on the detection signal of the turbidity sensor 14, the control unit 13 closes the fifth flow path opening / closing means 85,
In addition, the third channel opening / closing means 83 is opened, and the plasma is collected in the plasma collection bag 25. The plasma collection bag 25
The weight is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. For this reason, when the weight of the plasma collected in the plasma collection bag 25 increases by a predetermined amount (for example, 30 g), the control unit 13 closes the first channel opening / closing unit 81 and the second channel opening / closing unit 82
Is released, and the operation proceeds to the constant speed circulation step.

Then, the above-mentioned constant speed circulation step, the second
The plasma collection step and the accelerated circulation step of FIG.
Then, the blood collecting step for adjusting the interface, the platelet collecting step, and the blood returning step are sequentially performed, and the final platelet collecting operation is completed. The difference between the last round and the second round is that the bag for inflowing the air in the above-described constant-speed circulation step is different, and in the last round, the blood return step is performed without performing the buffy coat collection step and the blood return step. It is the contents of.

For particle suspensions, it is known that the higher the particle concentration, the higher the apparent viscosity of the suspension. The higher the viscosity, the smaller the sedimentation velocity difference between particles with different specific gravities. When this is applied to the platelet collection step, as the viscosity in the centrifuge 20 increases, the probability that white blood cells having a higher specific gravity than platelets flow out together with platelets increases. During blood collection, the centrifuge 20 is rotating, and the blood components in the centrifuge 20 are constantly subjected to centrifugal force. From the outside of the vessel, it is separated into a blood cell layer, a buffy coat layer, and a plasma layer as shown in FIG. The particles in each layer are sequentially smaller from the outside.

A plurality of cake layers are formed in the centrifugal separator 20, and the cake layers are gradually compressed.
Then, the newly flowing whole blood flows in from the cake layer formed by the outermost blood cells of the centrifugal separator 20, and the blood cells are caught by this cake layer and other components pass through, and the buffy coat layer and the plasma layer Move to However, when the blood cell cake layer and the buffy coat cake layer are excessively compressed, the gap between the fine particles in each layer becomes small, and platelets are trapped in the blood cell cake layer or the buffy coat cake layer. For this reason, it is expected that the collection efficiency of platelets will decrease.

However, in the present invention, the plasma collection step can be divided and the time of the first plasma collection step can be shortened, and the accelerated plasma circulation step is performed after the second plasma collection, thereby compressing the above-mentioned cake layer. Progress is difficult. In particular, even if the final reaching circulation speed is increased by using the accelerated plasma circulation step, not only does the cake layer hardly compress, but also the CRC layer (rich red blood cell layer)
Platelets buried in the cell can be raised and taken up in the BC layer. Further, since the BC layer itself also rises, the separation and alignment of platelets and WBC (white blood cells) in the BC layer are promoted.
Thus, the collection efficiency of platelets is improved.

Finally, a blood return step (blood return step) for returning the blood in the centrifuge 20 is performed. In the blood return step, the process proceeds to FIG. In this embodiment, the blood plasma is returned together with the blood cell components in the centrifuge 20. Therefore, the blood return step is a step of performing both blood cell component return and plasma return. First, the flow path opening / closing means 82, the third flow path opening / closing means 83, the fourth flow path opening / closing means 84, the fifth flow path opening / closing means 85, the sixth flow path opening / closing means 86, and the seventh flow path The opening / closing means 87 is closed, only the first flow path opening / closing means 81 is opened, and the blood pump 11 rotates in the reverse direction. Thereby, the blood cell component in the centrifuge 20
The blood passes through the tubes 21b and 21a and is returned to the donor via the blood collection needle 29. And this blood cell component return,
As shown in FIG. 13, the operation is stopped when the amount of blood returned reaches one predetermined amount of blood returned (for example, 50 ml). The flow rate is measured by the number of rotations of the pump. Subsequently, plasma return is performed. In this plasma return, the plasma is not directly returned to the blood donor, but is made to flow into the centrifuge 20. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. In the plasma return, it is determined whether the plasma weight (PPP) in the plasma collection bag 25 is the target plasma collection amount (for example, 200 g), and if the plasma weight (PPP) is larger than the target plasma collection amount,
Excess plasma is returned to the donor and the plasma weight (PPP)
However, when the amount of the collected plasma is equal to or less than the target plasma collection amount, the blood cell component is returned without performing the plasma return. Then, when the plasma return is performed, it is determined whether or not the plasma return amount has reached a set return plasma amount (for example, 50 g) at one time. Weight (PP
If P) is equal to or greater than the target plasma collection volume, the return of plasma is stopped and the blood cell component is returned again. Then, the above steps are repeated, and when the plasma weight (PPP) in the plasma collection bag 25 reaches the target plasma collection amount, it is determined whether or not the blood cell component return is completed (specifically, tube Sensor 31 attached to 21b
(For example, ultrasonic sensor, optical sensor, infrared sensor)
Detects whether there is a blood component in the tube). Then, when the presence of the blood component in the tube is confirmed, the blood cell component return is started, and it is finally confirmed that the blood cell component return end state has been reached, and the blood return step ends.

As a result, the red blood cell layer remaining in the centrifuge 20 and the plasma having the target plasma collection amount or more are returned to the donor. Thus, all platelet collection operations are completed. In the above embodiment, the plasma is also returned in the final blood return step. However, the present invention is not limited to this. In the blood return step in the final platelet collection operation, the controller returns the blood return in the final blood return step. The control may be such that the plasma collected in the plasma collection bag is not returned by the step.

(Embodiment 2) The control unit 13
As in the embodiments shown in FIG. 14 to FIG. 22, the present invention is not limited to the type in which the rotor speed is calculated in all cases. For example, when the input hematocrit value is less than 40% or 50%. Only when it exceeds, the rotor speed suitable for the hematocrit value is calculated, and the rotor speed is controlled when necessary using the calculated value.If the rotor speed is not calculated, that is, the input hematocrit value is When it is 40% or more and 50% or less, the rotor rotation may be controlled at a preset rotor rotation number stored in advance. Except for this point, it is the same as the above-described embodiment.

In this case, the control unit 13 includes a hematocrit value input unit, and determines whether or not the input hematocrit value is less than 40% or exceeds 50% (rotor speed calculation necessity determination function). When the judgment function judges that the calculation of the rotor speed is necessary,
Calculation of the rotor speed suitable for the hematocrit value and the rotor speed calculation and output function that outputs the calculated value, and the judgment function determine that the rotor calculation is unnecessary (input hematocrit value is 40% or more and 50% or less) It has a set rotor speed storage and output function that is output when the setting is performed. When necessary (at the time of the plasma collection step, the plasma collection / constant-speed circulation step, the second plasma collection step, and the plasma collection / acceleration circulation step), the control unit performs any one of the output functions described above. And controls the centrifugal separator driving device to rotate the rotor according to the rotor speed output by

FIG. 4 shows a blood component collecting step (first platelet collecting operation) by the blood component collecting apparatus 1 of this embodiment.
This will be described with reference to the flowcharts of FIGS. In this embodiment, the platelet collection operation is repeated three times, and after the end of the platelet collection step other than the last one, before the blood return step, the buffy coat collection step is performed and before the next blood collection step. Centrifuge 20
A buffy coat return step for returning this is performed.

First, the third line 23 and the blood collection needle 29 are primed with an anticoagulant, and then the donor is punctured with a puncture needle. Then, a small amount of blood is collected, a hematocrit value is measured by a hematocrit measuring device prepared separately, and the value is input from a hematocrit value input unit as shown in FIG. The control unit determines whether it is necessary to calculate the rotor rotation speed based on the input hematocrit value, specifically, the input hematocrit value is 40%
It is determined whether it is not less than 50% or less, and if it is not applicable (in the case of N), an appropriate rotor speed is calculated. For this reason, the rotation of the rotor is performed with the calculated value when there is a calculated value, and with the set value when there is no calculated value.

The first liquid feed pump 11 and the second liquid feed pump 12 are operated to collect blood to which the anticoagulant has been added,
The centrifugal separator driving device 10 uses the calculated value if there is a calculated value of the rotor speed, and if there is no calculated value of the rotor speed, the centrifuge driving device 10 uses the set number of revolutions stored in the control unit. Is rotated to perform a first plasma collection step of collecting a first predetermined amount of plasma from the blood into the plasma collection bag 25. When the first blood collection is started, the blood pump 11
Starts blood collection at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is simultaneously driven at a predetermined speed (for example, 1/10 of the blood pump speed).
Supplies an anticoagulant (for example, ACD-A solution). The blood collected from the donor is mixed with the ACD solution, flows through the first line 21, and is connected to the chamber, the first channel opening / closing means 81.
And flows into the centrifuge 20. At this time, the sixth
, The fifth passage opening / closing unit 85, the second passage opening / closing unit 82, the third passage opening / closing unit 83, and the seventh passage opening / closing unit 87 are closed. Channel opening / closing means 8
The first and fourth flow path opening / closing means 84 are open. When the ACD blood is supplied to the centrifuge 20, the sterilized air that has entered the centrifuge 20 flows through the second line 22, passes through the fourth channel opening / closing means 84, and enters the platelet collection bag 26. Flows into. Simultaneously with the start of the blood collection process, the rotor of the centrifugal separator 20 is operated or set (for example, 4800 rpm).
m), the centrifugal separator 20 rotates while rotating A
Since the blood is supplied with CD blood, the blood is centrifuged in the separator, and the blood is separated from the inside into three layers, a plasma layer, a buffy coat layer (BC layer), and a red blood cell layer, and the capacity of the separator is increased. When ACD blood (about 270 ml) is supplied, the centrifuge 20 is completely filled with blood.
Plasma flows out of the outlet of the centrifuge 20. The turbidity sensor 14 attached to the second line 22 connected to the outlet of the centrifuge 20 detects that the fluid flowing in the line
Upon detecting that the air has changed to plasma, the control unit 13
Based on the detection signal of the turbidity sensor 14, the fourth flow path opening / closing means 84 is closed and the third flow path opening / closing means 83 is opened, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. For this reason, when the weight of the plasma collected in the plasma collection bag 25 increases by the first predetermined amount (10 to 150 g, for example, 20 g), the control unit 13 closes the first flow path opening / closing means 81, and Then, the flow path opening / closing means 82 is opened to shift to a constant speed circulation step.

In the constant-speed circulation step, the blood collection is temporarily stopped, and the centrifugal separator driving device 10 is operated to maintain the rotation of the rotor of the centrifugal separator 20 at the calculated value or the set value. The plasma collected therein is circulated through the centrifuge 20 at a constant speed. When entering the constant-speed circulation step, the control unit 13 maintains the closed state of the first flow path opening / closing means 81 and the open state of the second flow path opening / closing means 82, stops the ACD pump 12, and stops the blood pump 11 Is a predetermined speed (60 to 250 ml / min, for example, 200 ml
/ Min), and the plasma in the plasma collection bag 25 is
Centrifuge 2 rotating at a calculated value or a set value (for example, 4800 rpm)
Sent to 0. At the same time, the plasma flowing out of the centrifuge 20 flows into the plasma collection bag 25 through the turbidity sensor 14 and the third channel opening / closing means 83. When a predetermined time (10 to 90 seconds, for example, 30 seconds) elapses after the start of the constant speed circulation step, the control unit 13 closes the second flow path opening / closing means 82 and opens the first flow path opening / closing means 81. Then, the process proceeds to the second plasma collection step. The first plasma circulation is preferably performed at a flow rate of at least 60 ml / min or more for 10 seconds or more.

In the second plasma collection step, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added, and the amount of plasma in the bag is increased. When the optical sensor 15 detects the buffy coat layer of the separator, this signal is sent to the control unit 13, and the control unit 13 closes the first flow path opening / closing means 81 and opens / closes the second flow path. The means 82 is opened to move to the accelerated plasma circulation step. Specifically, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min).
At this time, the second pump, which is an anticoagulant pump, simultaneously supplies the anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). The blood collected from the donor is mixed with the ACD solution, flows into the centrifugal separator 20 where the rotor rotates at a calculated value or a set value (for example, 4800 rpm), and converts the plasma into a plasma collection bag 25.
Collect inside. Usually, due to the increased plasma volume in the bag,
When the optical sensor 15 detects the buffy coat layer of the separator, this signal is sent to the control unit 13 and the control unit 13
Closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to an accelerated plasma circulation step. In the plasma collection step, plasma is collected until the sensor detects a buffy coat (BC interface: interface between the plasma layer and the buffy coat layer). In the apparatus of this embodiment, as shown in the flowcharts of FIGS. 14, 17, and 20, the BC interface is also detected in each of the first to final plasma collection steps, if,
If a BC interface is detected during these first through last plasma collection steps, plasma collection is interrupted and the process proceeds to an accelerated plasma circulation step.

In the accelerated circulation step, the blood collection is temporarily stopped, and the centrifuge drive device 10 is operated to maintain the rotation of the rotor of the centrifuge 20 at the calculated value or the set value. Centrifuge 20
Then, circulate while accelerating. At this time, the blood pump speed is lower than the constant speed circulation step, for example, 60 m
Start at 1 / min, final speed 200ml / mi
Accelerate until n is reached. The acceleration conditions are as follows: 6.7 ml / min speed increases every second, 200 ml
This is carried out with a / min arrival time of about 21 seconds. After the completion of the accelerated circulation step, the flow shifts to that in FIG. 15, and a blood collection step for adjusting the interface is performed.

As shown in FIG. 15, the blood collecting step for adjusting the interface can be said to be a small-quantity plasma collecting step. In this step, in order to keep the position of the buffy coat layer constant in the subsequent platelet collection step regardless of the donor, blood is collected by a predetermined supply amount of red blood cells. The red blood cell supply amount is defined as a value obtained by dividing the blood collection amount by the hematocrit value of the donor, and the blood collection amount is generally about 12 ml. Also in this blood collection, the first liquid supply pump 11 operates at a predetermined speed (for example, 60 m).
1 / min). At this time, the second pump, which is an anticoagulant pump, also simultaneously transmits the anticoagulant (for example, ACD) at a predetermined speed (for example, 1/10 of the blood pump speed).
-A solution). Blood collected from the donor was AC
It is mixed with the liquid D, and the calculated value or set value (for example, 48
(00 rpm) and a small amount of plasma is collected. The control unit 13 calculates the collection time from the set collection amount and the pump speed, and ends the blood collection when the collection time has elapsed. Then, the control unit 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a platelet collection step.

After the above steps are completed, the circulating speed of the plasma by the first liquid feed pump 11 is accelerated, and the centrifuge 20
Platelets are drained from the inside and platelets are collected in a platelet collection bag 26.
A platelet collection step of collecting platelets. The platelet collection step is also called a so-called acceleration process. In this step, the blood pump speed is, in this example, 60 ml /
The control unit 13 operates the blood pump so as to accelerate the blood pump from 10 min / min to 200 ml / min every predetermined time (for example, 1 second) by 10 ml / min.
Once in is reached, the speed is maintained until the platelet collection step is completed.

When the platelet collection step starts, the turbidity sensor 14 detects the turbidity of the liquid passing therethrough, and the turbidity is output as a voltage value by the sensor.
3 is input. When the speed of the blood pump increases and reaches approximately 160 to 200 ml / min, platelets contained in the buffy coat layer remaining in the centrifuge 20 flow out. When the platelets flow out, the turbidity of the liquid passing through the turbidity sensor 14 increases, and when the voltage value output from the sensor drops by 0.2 V, the third flow path opening / closing means 83 closes and the fourth flow The path opening / closing means 84 is opened, and platelet-rich plasma flowing out of the centrifuge 20 is collected in the platelet collection bag 26. The voltage value output from the turbidity sensor 14 is converted into a platelet concentration by the control unit 13, and the platelet concentration in the platelet collection bag 26 during platelet collection is calculated. The platelet concentration in the platelet collection bag 26 once reaches the maximum concentration and then decreases. At the point in time when it is detected that the maximum concentration has been reached, the platelet collection step ends, and the process proceeds to the buffy coat collection step.

In the buffy coat collection step, when the above-described platelet collection step is completed, the control unit 13
Is closed, and the fifth channel opening / closing means 85 is opened. The blood plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 by the blood pump 11, and the liquid (the buffy coat layer has flowed out) from the centrifuge 20 simultaneously flows into the buffy coat collection bag 27. .
In the buffy coat collection step, the blood pump speed is maintained at the final speed in the platelet collection step. At the time when the amount calculated from the hematocrit value of the toner and the amount of collected platelets is collected, the blood pump 11 is stopped, all valves are closed, the rotation of the rotor of the centrifuge 20 is stopped, and the buffy coat collection step is completed. .

Next, a blood return step (blood return step) for returning the blood in the centrifuge 20 is performed. In the blood return step,
It transfers to FIG. In this embodiment, the centrifuge 2
The plasma return is performed together with the return of the blood cell components within 0. Therefore, the blood return step is a step of performing plasma return and blood cell component return. In the blood return step, the weight of the plasma (ppp) in the plasma collection bag 25 in the first platelet collection step
1: The plasma weight in the plasma collection bag immediately before the first blood return step is stored in the control unit (in other words, the control unit 13 stores the plasma weight in the plasma collection bag immediately before the first blood return step). Has a memory function of the plasma weight ppp1), and the plasma weight (PPP) in the plasma collection bag 25 is
In the case of the minimum residual plasma amount A (for example, 50 g) or less,
Return blood cell components without returning plasma. Also,
The control unit 13 determines the number of platelet collection operations (1 because it is the first time).
× (target plasma collection amount-ppp1) / (total number of platelet collection operations-1) = B is provided, and plasma is returned even when the plasma weight (PPP) in the plasma collection bag 25 is less than or equal to the calculation value B Is performed, and control is performed so that the plasma is returned only when the plasma weight (PPP) in the plasma collection bag 25 is larger than the calculated value B. In addition,
It is rare that the calculated value B is smaller than the minimum remaining plasma volume A, so that the case where B is larger than A will be described below.

First, the flow path opening / closing means 82, the third flow path opening / closing means 83, the fourth flow path opening / closing means 84, the fifth flow path opening / closing means 85, the sixth flow path opening / closing means 86 and the seventh flow path Is closed, only the first channel opening / closing means 81 is opened, and the blood pump 11 rotates in the reverse direction. As a result, the blood cell components in the centrifuge 20 pass through the tubes 21b and 21a and are returned to the donor via the blood collection needle 29. This blood cell component return is shown in FIG.
As shown in FIG. 6, a predetermined amount of blood is returned once (for example,
50 ml). continue,
Plasma return is performed. In this plasma return, the plasma is not directly returned to the blood donor, but is made to flow into the centrifuge 20. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. In the plasma return, it is checked whether the plasma weight (PPP) in the plasma collection bag 25 is equal to or more than a predetermined required residual plasma amount (secured ppp, for example, 50 g). It is determined whether (PPP) is greater than the above-described calculated value B. If it is greater, it is determined whether the plasma return amount has reached a single set return plasma amount (for example, 50 g). Again, plasma collection bag 2
It is determined whether the plasma weight (PPP) in 5 is larger than the above-mentioned calculated value B, and if it is larger, the return of plasma is stopped and the blood cell component is returned again. Then, the above steps are repeated, and when the plasma weight (PPP) in the plasma collection bag 25 becomes less than or equal to the calculated value B (preferably, = calculated value B), it is determined whether or not the blood cell component return end state is reached. A determination is made (specifically, whether or not there is a blood component in the tube is detected by a sensor 31 (for example, an ultrasonic sensor, an optical sensor, or an infrared sensor) attached to the tube 21b). Then, when the presence of the blood component in the tube is confirmed, the blood cell component return is started, and it is finally confirmed that the blood cell component return end state has been reached, and the blood return step ends. As a result, the red blood cell layer remaining in the centrifuge 20 and the plasma in an amount exceeding the calculated value B are returned to the donor. As a result, the red blood cell layer remaining in the centrifuge 20 is returned to the donor through the first line 21. Thus, the first (first) platelet collection operation is completed.

Subsequently, the flow shifts to the second platelet collection operation shown in FIG. First, as shown in FIG.
A buffy coat return step of returning the buffy coat collected by the platelet collection steps to the centrifuge 20 before the next blood collection step is performed. In the buffy coat return step, the control unit 13 rotates the rotor of the centrifuge 20 with the calculated value or the set value, and controls the fifth channel opening / closing unit 85 and the fourth channel opening / closing unit 84.
, And set the blood pump 11 to the predetermined speed (default is 1
(00 ml / min). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 rotates by the amount of the buffy coat collected, the buffy coat return step ends.

Then, the first plasma collection step, the constant-speed circulation step, the second plasma collection step, the accelerated circulation step, the third plasma collection step, the third accelerated plasma circulation step, and the fourth plasma collection step are performed. After the step, the fourth accelerated plasma circulation step, the fifth plasma collection step, and the fifth accelerated plasma circulation step, the process proceeds to FIG.
The blood collection step for adjusting the interface, the platelet collection step, the buffy coat collection step, and the blood return step are sequentially performed.
The second platelet collection operation ends. In the blood return step,
The process proceeds to [10] in FIG. 19, where blood cell return and plasma return are performed in the same manner as in the first time, and the second platelet collection operation is completed. Note that the control unit 13 determines the number of platelet collection operations (2 because it is the second time) × (target plasma collection amount−ppp1)
/ (Total number of platelet collection operations −1) = B is calculated.

Next, the final platelet collection operation shown in FIG. 20 will be described. In this embodiment, the third time is the final time, but the present invention is not limited to this, and the fourth time and thereafter may be the final time of the platelet collection operation. In this case, except for the last round, the second platelet collection operation (FIG. 17,
18 and 19). First, as shown in FIG. 20, the buffy coat collected by the second platelet collection operation was centrifuged before the next blood collection step.
Perform a buffy coat return step to return within 0. When the process proceeds to the buffy coat return step, the control unit 13
Rotates the rotor of the centrifuge 20 at the calculated value or the set value, opens the fifth flow path opening / closing means 85 and the fourth flow path opening / closing means 84, and starts the blood pump 11 at a predetermined speed (the default is 100 ml). / Min). The buffy coat contained in the buffy coat collection bag 27 is
It is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 passes through a second line 22,
The platelet collection bag 26 passes through the fourth flow path opening / closing means 84.
Sent to Blood pump 1 for the amount of buffy coat collected
After 1 rotates, the buffy coat return step ends.

Next, the rotation of the rotor of the centrifuge 20 at the calculated value or the set value is maintained, and the first liquid feeding pump 1 is rotated.
1. A first plasma collection step of operating the second liquid supply pump 12 to collect blood to which an anticoagulant has been added, and collecting a first predetermined amount of plasma from the blood into a plasma collection bag 25. Do. When blood collection is started, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, simultaneously supplies the anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the blood pump speed). The blood collected from the donor is mixed with the ACD solution, flows through the first line 21, passes through the chamber and the first channel opening / closing means 81, and flows into the centrifuge 20. At this time, the sixth channel opening / closing unit 86, the fifth channel opening / closing unit 85, the second channel opening / closing unit 82, the third channel opening / closing unit 83, and the seventh channel opening / closing unit 87 are closed. The first channel opening / closing means 81;
The fifth channel opening / closing means 85 is open, and the centrifuge 20
Is supplied with the ACD blood, the sterilized air originally contained in the centrifuge 20 flows into the buffy coat collection bag 27 through the line sensor and the fifth flow path opening / closing means 85. Since the centrifuge 20 keeps rotating at a predetermined speed (for example, 4800 rpm) simultaneously with the start of the blood collection step, and the centrifuge 20 receives the supply of ACD blood while rotating,
Blood is centrifuged in the separator, and the blood is separated from the inside into a plasma layer, a buffy coat layer (BC layer), and a red blood cell layer.
When the ACD blood (about 270 ml) which is separated into layers and exceeds the capacity of the separator is supplied, the inside of the centrifuge 20 is completely filled with blood, and the plasma flows out from the outlet of the centrifuge 20. The second connected to the outlet of the centrifuge 20
The turbidity sensor 14 attached to the line 22 detects that the fluid flowing in the line has changed from air to plasma, and the control unit 13 performs the fifth operation based on the detection signal of the turbidity sensor 14. The flow path opening / closing means 85 is closed, and the third
Is opened, and the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. Therefore, when the weight of the plasma collected in the plasma collection bag 25 increases by a predetermined amount (for example, 30 g), the control unit 13 closes the first channel opening / closing unit 81 and sets the second channel opening / closing unit 82 to Release and move to the constant speed circulation step.

Then, the above-mentioned constant speed circulation step, the second
The plasma collection step and the accelerated circulation step of FIG.
[11], the blood sampling step for interface adjustment,
The platelet collection step and the blood return step are sequentially performed, and the final platelet collection operation is completed. The difference between the last round and the second round is that the bag for inflowing the air in the above-described constant-speed circulation step is different, and in the last round, the blood return step is performed without performing the buffy coat collection step and the blood return step. It is the contents of. Finally, the centrifuge 20
A blood return step (blood return step) for returning the blood in the inside is performed.
In the blood return step in the final platelet collection operation, if the weight of the plasma collected by the final blood return step exceeds the target plasma collection amount, excess plasma is returned. That is, if the weight of the plasma collected before the blood return step in the final platelet collection operation is equal to or less than the target plasma collection amount, the plasma is not returned. Note that the present invention is not limited to such an embodiment, and the plasma may not be returned in the final blood return step regardless of the final plasma weight.

In this embodiment, the blood return step is performed as shown in FIG.
Is started by shifting to [12]. In this embodiment, the blood plasma is returned together with the blood cell components in the centrifuge 20. Therefore, the blood return step is a step of performing both blood cell component return and plasma return. First, the flow path opening / closing means 82, the third flow path opening / closing means 83, the fourth flow path opening / closing means 84, the fifth flow path opening / closing means 85, the sixth flow path opening / closing means 86, and the seventh flow path The opening / closing means 87 is closed, only the first flow path opening / closing means 81 is opened, and the blood pump 11 rotates in the reverse direction. Thereby, the centrifuge 20
The blood cell components inside pass through the tubes 21b and 21a and are returned to the donor via the blood collection needle 29. Then, as shown in FIG. 22, the blood cell component return is stopped when the blood return volume reaches a predetermined predetermined blood return volume (for example, 50 ml). Subsequently, plasma return is performed. In this plasma return, the plasma is not directly returned to the blood donor, but is made to flow into the centrifuge 20. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13. In the plasma return, the plasma weight (PPP) in the plasma collection bag 25 is changed to the target plasma collection amount (for example, 200
g) and if the plasma weight (PPP) is greater than the target plasma collection volume, the excess plasma is returned to the donor and the plasma weight (PPP) is less than or equal to the target plasma collection volume. In this case, the blood cell component is returned without performing the plasma return. Then, when the plasma return is performed, it is determined whether or not the plasma return amount has reached a set return plasma amount (for example, 50 g) at one time. If the weight (PPP) is equal to or greater than the target plasma collection amount, the return of plasma is stopped, and the blood cell component is returned again. And repeat the above steps,
When the plasma weight (PPP) in the plasma collection bag 25 reaches the target plasma collection amount, it is determined whether or not the blood cell component return end state has been reached (specifically, the sensor 31 attached to the tube 21b (for example, , An ultrasonic sensor, an optical sensor or an infrared sensor) to detect whether there is a blood component in the tube). Then, when the presence of the blood component in the tube is confirmed, the blood cell component return is started, and it is finally confirmed that the blood cell component return end state has been reached, and the blood return step ends. As a result, the red blood cell layer remaining in the centrifugal separator 20 and the plasma having the target plasma collection amount or more are returned to the donor. Thus, all platelet collection operations are completed.

[0091]

According to the blood component collecting apparatus of the present invention, since the apparatus is constituted by two pumps, the apparatus can be downsized. Further, during one platelet collection operation, at least two plasma recirculation steps of suspending blood collection and recirculating the collected plasma to the centrifuge are performed, and the latter half of the plasma recirculation step is performed. Because of the accelerated circulation,
The blood cell layer and the buffy coat layer in the centrifuge can be prevented from being excessively compressed, and platelets buried in the dense red blood cell layer can be raised and taken into the buffy coat layer. In addition, the buffy coat layer itself soars, thereby promoting the separation and alignment of platelets and leukocytes in the buffy coat layer. Therefore, it is possible to obtain a platelet-containing liquid (rich platelet plasma) with little leukocyte contamination and high platelet collection efficiency.

Further, in the blood component collecting apparatus of the present invention,
In the blood return step in the platelet collection operation before the last round, the control unit returns the plasma until the weight of the plasma collected before the blood return step reaches the first predetermined amount (required minimum remaining plasma amount). It is controlled so that In this way, at the end of each platelet collection operation before the last round, by leaving only the required minimum amount of collected plasma and returning the rest, the amount of the returned plasma at the end of each platelet collection operation (in other words, For example, the blood removal amount at the time of the next platelet collection operation start) becomes smaller by that amount, and the burden on the donor is reduced. Furthermore, if plasma is not returned only in the last round, or if a predetermined amount is left, plasma can be collected together with platelets, which can be effectively used as a raw material of a plasma preparation.

In the blood return step in the platelet collection operation before the last round, the control unit returns the blood and the weight of the plasma collected before the blood return step (the plasma collection bag 25).
The first liquid feed pump 11 and the plurality of flow path opening / closing means are controlled so that the plasma is returned so that the plasma weight within the range falls within the range of the residual plasma volume (y) under the following conditions. You may. (Conditions) The residual plasma volume (y) is equal to or higher than A and equal to or lower than B when A ≦ B. A = required minimum residual plasma volume B = number of platelet collection operations × (target plasma collection amount−1 end of first platelet collection operation) The amount of plasma collected at the time) / (total number of platelet collection operations-1) By controlling in this way, it is possible to collect plasma at or near the target plasma collection volume, and at the same time, before the final platelet collection operation. Under normal conditions, the amount of plasma collected is smaller than in the method of collecting an equal amount of plasma, the amount of blood removed before the final round is reduced, and the burden on the donor can be reduced.

[Brief description of the drawings]

FIG. 1 is a plan view showing a configuration example of a blood component collection circuit used in a blood component collection device of the present invention.

FIG. 2 is a plan view of a cassette housing part of the blood component collection circuit of FIG. 1;

FIG. 3 is a partially broken cross-sectional view showing a state in which a driving device is mounted on a centrifuge used in a blood component collection circuit.

FIG. 4 is a conceptual diagram of one embodiment of a blood component collecting apparatus of the present invention in a state where a blood component collecting circuit is mounted.

FIG. 5 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 6 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 7 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 8 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 9 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 10 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 11 is a flowchart for explaining the operation of a blood component collecting apparatus according to another embodiment of the present invention.

FIG. 12 is a flowchart for explaining the operation of a blood component collecting apparatus according to another embodiment of the present invention.

FIG. 13 is a flowchart for explaining the operation of the blood component collecting apparatus according to another embodiment of the present invention.

FIG. 14 is a flowchart for explaining the operation of a blood component collecting apparatus according to another embodiment of the present invention.

FIG. 15 is a flowchart for explaining the operation of the blood component collecting apparatus according to another embodiment of the present invention.

FIG. 16 is a flowchart for explaining the operation of a blood component collecting apparatus according to another embodiment of the present invention.

FIG. 17 is a flowchart for explaining the operation of the blood component collecting apparatus according to another embodiment of the present invention.

FIG. 18 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 19 is a flowchart for explaining the operation of the blood component sampling device of the present invention.

FIG. 20 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 21 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

FIG. 22 is a flowchart for explaining the operation of the blood component collecting apparatus of the present invention.

[Explanation of symbols]

 Reference Signs List 1 blood component collection device 2 blood component collection circuit 10 centrifuge drive device 11 first liquid feed pump 12 second liquid feed pump 13 control unit 14 turbidity sensor 15 optical sensor 16 weight sensor 20 centrifuge 21 1 line 22 2nd line 23 3rd line 24 4th line 25 Plasma collection bag 26 Platelet collection bag 29 Blood collection needle

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (reference) G01N 33/48 A61B 5/14 300G 33/49 300C 310 F term (reference) 2G045 AA06 BA10 BB10 BB34 CA02 CA11 CA24 CA25 CA26 FA05 GA05 HA06 HA14 JA01 JA07 JA08 JA11 4C038 KK00 KM00 KX01 TA04 UA03 UA06 UA10 UG02 UG05 UG10 4C077 AA13 BB04 CC09 DD07 DD13 DD27 EE01 FF01 HH03 HH16 HH20 JJ03 KK07 JJ13 JJ07 JJ13 JJ19 JJ13 JJ07 JJ30

Claims (13)

[Claims] A rotor having a blood storage space therein, and an inlet and an outlet communicating with the blood storage space, and centrifuging the blood introduced from the inlet by rotation of the rotor in the blood storage space. A centrifuge for separation, a first line for connecting a blood collection needle or a blood collection device connection portion to an inlet of the centrifuge, and a second line connected to the outlet of the centrifuge A third line connected to the first line for injecting an anticoagulant; a second tube connected to the first line and the second line connected in the middle of the first line; A blood component collection device including a blood component collection circuit including a plasma collection bag having a tube and a platelet collection bag connected to the second line, wherein the blood component collection device includes a centrifugal separator. The rotor A centrifugal separator driving device for rotating the first line, a first liquid sending pump for the first line, which is disposed closer to the centrifugal separator than a connection portion between the first line and the first tube; A second liquid feed pump for the third line, a plurality of flow path opening / closing means for opening / closing a flow path of the blood component collection circuit, the centrifugal separator driving device, the first A control unit for controlling the liquid supply pump, the second liquid supply pump, and the plurality of flow path opening / closing units, wherein the control unit collects blood to which an anticoagulant is added; A plasma collection step of collecting the separated and separated plasma into the plasma collection bag, and a plasma circulation step of circulating the plasma in the plasma collection bag collected by the plasma collection step to the centrifuge, Blood performed at least once A collecting and circulating step; after the plasma collecting and circulating step is completed, a platelet collecting step of accelerating a plasma circulating speed, causing platelets to flow out of the centrifuge and collecting platelets in the platelet collecting bag, After completion of the collection step, the centrifuge drive device, the first liquid feed pump, the blood pump, so that a platelet collection operation for performing a blood return step of returning blood in the centrifuge is performed a plurality of times. And controlling the second liquid sending pump and the plurality of flow path opening / closing means. In the blood return step in the platelet collection operation before the last round, the weight of the plasma collected before the blood return step is a predetermined value. A blood component collection device for controlling the return of plasma to a required residual plasma volume. 2. A rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and blood introduced from the inlet by rotation of the rotor is centrifuged in the blood storage space. A first line for connecting a centrifuge to be separated, a blood collection needle or a blood collection device connection portion to an inlet of the centrifuge, and a second line connected to the outlet of the centrifuge A third line connected to the first line for injecting an anticoagulant; a second tube connected to the first line and a second line connected in the middle of the first line; A blood component collection device provided with a blood component collection circuit comprising a plasma collection bag having a tube and a platelet collection bag connected to the second line, wherein the blood component collection device includes a centrifugal separator. The rotor A centrifugal separator driving device for rotating the first line, a first liquid sending pump for the first line, which is disposed closer to the centrifugal separator than a connection portion between the first line and the first tube; A second liquid feed pump for the third line, a plurality of flow path opening / closing means for opening / closing a flow path of the blood component collection circuit, the centrifugal separator driving device, the first A control unit for controlling the liquid supply pump, the second liquid supply pump, and the plurality of flow path opening / closing units, wherein the control unit collects blood to which an anticoagulant is added; A plasma collection step of collecting the separated and separated plasma into the plasma collection bag, and a plasma circulation step of circulating the plasma in the plasma collection bag collected by the plasma collection step to the centrifuge, Blood performed at least once A collecting and circulating step; after the plasma collecting and circulating step is completed, a platelet collecting step of accelerating a plasma circulating speed, causing platelets to flow out of the centrifuge and collecting platelets into the platelet collecting bag, After completion of the collection step, the centrifuge drive device, the first liquid transfer pump, and the platelet collection operation are performed a plurality of times to perform a blood return step of returning blood in the centrifuge. And controlling the second liquid sending pump and the plurality of flow path opening / closing means. In the blood return step in the platelet collection operation before the last round, the weight of the plasma collected before the blood return step is as follows: A blood component collecting apparatus for controlling the return of plasma so as to fall within the range of the residual plasma volume (y) of the condition. (Condition) The residual plasma volume (y) is not less than A and not more than B when A ≦ B. A = predetermined required residual plasma volume B = number of platelet collection operations × (target plasma collection volume—before the first blood return step) Of plasma collected at the end of platelet collection
/ (Total number of platelet collection operations -1) 3. The control section controls the plasma collection / circulation step to be performed twice. In the first plasma collection / circulation step, the plasma in the plasma collection bag is separated by the centrifugal separator. Plasma collection at constant speed
A constant-speed circulation step is performed, and in the second plasma collection / circulation step, control is performed such that a plasma collection / accelerated circulation step of circulating plasma in the plasma collection bag while accelerating the plasma in the centrifuge is performed. The blood component collection device according to claim 1 or 2, wherein 4. The control unit, in a blood return step in a final platelet collection operation, if the weight of plasma collected by the final blood return step exceeds the target plasma collection amount, 4. The blood component collection device according to claim 1, wherein the blood component collection device is controlled to return the plasma. 5. The control unit controls in a blood return step in a final platelet collection operation not to return the plasma collected in the plasma collection bag by the final blood return step. The blood component collection device according to any one of claims 1 to 3. 6. The predetermined required residual plasma amount is 10 to 50.
5. The blood component collecting apparatus according to claim 1, wherein the blood component is ml. 7. The blood component collection device according to claim 1, wherein the control unit has a function of controlling a centrifugal rotation speed of a rotor of the centrifuge according to a hematocrit value of a blood donor. . 8. The blood component collection device includes a hematocrit value input unit or a hematocrit value measurement function,
Using the input or measured hematocrit value, the control unit uses a rotor rotation speed calculation function for calculating a rotor rotation speed suitable for the hematocrit value and a rotor rotation speed calculated by the rotor rotation speed calculation function. The blood component collection device according to claim 7, further comprising a function of controlling the centrifuge driving device. 9. The blood component collection device according to claim 1, wherein the blood component collection device includes a weight sensor for the plasma collection bag. 10. The centrifugal separator driving device, the first liquid feed pump, the second liquid feed pump, and the plurality of flows so that the platelet collection operation is performed at least twice. 9. The blood component collecting apparatus according to claim 1, wherein the apparatus controls a road opening / closing means. 11. The blood component collection circuit includes a buffy coat collection bag connected to the second line,
The control unit, after the end of the platelet collection step, before the blood return step, the plasma circulation speed by the first liquid pump is higher than the final speed in the platelet collection step, the centrifuge The blood component collection device according to any one of claims 1 to 10, wherein a buffy coat collecting step is performed for causing a buffy coat to flow out from the inside and collecting the buffy coat in the buffy coat collection bag. 12. The blood component collection circuit includes a buffy coat collection bag connected to the second line,
The controller, after the end of the platelet collection step and before the blood return step, makes the plasma circulation speed of the first liquid feeding pump equal to or higher than the final speed in the platelet collection step, and performs the centrifugation. The buffy coat collecting step of lowering the rotation speed of the rotor of the separator, causing the buffy coat to flow out of the centrifugal separator, and collecting the buffy coat in the buffy coat collection bag, is performed. The blood component sampling device according to claim 1. 13. The control unit returns the collected buffy coat to the centrifugal separator before the next blood collection step, if a platelet collection operation is performed after the completion of the buffy coat collection step. 13. The blood component collecting apparatus according to claim 11, wherein the first liquid feed pump and the plurality of flow path opening / closing means are controlled so as to perform a buffy coat return step.