JP2002126072A - Platelet collection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a platelet collection device which enables a user to confirm, during the collection of platelets, a more accurate compensatory number of platelets to be collected than the expected number of platelets to be collected in the scheduled number of cycles of platelet procedures. SOLUTION: A platelet collection device 1 is a device that calculates the number of platelet collection cycles and the expected number of platelets to be collected or their associated levels from the hematocrit data, the platelet concentration data and the target collection number of platelets of a donor to be inputted prior to the procedure of collecting platelets. The platelet collection device 1 is provided with a function to calculate the expected compensatory number of platelets to be collected for calculating the compensatory level of the expected platelets to be collected, using the expected extracorporeal circulatory blood volume and the expected number of platelets to be collected which are calculated from an actual extracorporeal circulatory blood volume during the first operational procedure of platelet collection following the termination of blood collection for the first operational procedure of platelet collection and the above hematocrit data.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a platelet collecting apparatus for collecting platelets from blood.

[0002]

2. Description of the Related Art At the time of performing blood collection, at present, blood is separated into blood components by centrifugation or the like, for reasons such as effective use of blood and reduction of the burden on donors, and only the components necessary for the transfuser are collected. Samples are collected and other components are collected and returned to donors. In such a component collection, blood collected from a donor is introduced into a platelet collection circuit, and separated into four components, plasma, white blood cells, platelets, and red blood cells, by a centrifuge called a centrifugal bowl installed in the platelet collection circuit. Then, the platelets are collected in a container, and the platelet product and plasma are also collected in another container to be used as a raw material for a plasma product or a plasma fraction product, and the remaining plasma, white blood cells and red blood cells are returned to the donor. Is done.

As a method for collecting platelets, see, for example,
As disclosed in Japanese Unexamined Patent Publication No. 313599, blood is preliminarily collected from a donor before blood collection, blood information (hematocrit value, platelet concentration) is obtained from the preliminarily collected blood, and the blood information data and the target platelet collection number are calculated. The number of required platelet collection operation cycles is calculated by inputting to the platelet collection device, and the platelet collection operation of the cycle number is performed.

[0004]

However, the measurement error of the analyzer for obtaining blood information from the pre-collected blood,
Input errors to the platelet collection device may occur,
In this case, the number of platelet collection cycles is calculated based on the incorrect blood information data, and the platelet collection operation is performed at that cycle number. For this reason, although the operator believed that the target platelet count could be collected, the inspection after collection revealed that the target platelet count was not actually collected for the first time. . An object of the present invention is to calculate the expected number of platelets to be collected in the scheduled number of platelet operation cycles, using data and the like obtained from the first platelet collection operation after the end of the blood collection in the first platelet collection operation, An object of the present invention is to provide a platelet collection device that allows an operator to confirm a newly calculated predicted platelet collection number during a platelet collection operation.

[0005]

Means for achieving the above object are as follows: (1) A rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and the rotation of the rotor A centrifugal separator for centrifuging the blood introduced from the inflow port in the blood storage space, a first line for connecting a blood collection needle or a blood collection device connection to an inflow port of the centrifuge, A second line connected to the outlet of the centrifuge; a third line for anticoagulant injection connected to the first line;
A platelet collection circuit comprising a plasma collection bag having a first tube connected in the middle of the line and a second tube connected to the second line, and a platelet collection bag connected to the second line. A blood supply pump provided on the first line, and based on the blood donor's hematocrit value data and platelet concentration data and the target platelet collection number or its related value input before the platelet collection operation, the number of platelet collection cycles And a platelet collection device for calculating the number of platelets to be collected or a related value thereof, the platelet collection device comprising: after the blood collection in the first platelet collection operation, the actual extracorporeal blood in the first platelet collection operation; Using the blood volume value, the predicted extracorporeal blood volume value calculated from the hematocrit value data, and the predicted blood platelet count, collection A platelet collection device comprising a correction sampling predicted platelet count calculation function for calculating a correction value of the number of platelets.

(2) In the above (1), the platelet collection device operates the liquid supply pump from the start of blood collection to the time when the centrifuge is filled with anticoagulant-added blood and the stored unit operation amount. Using the calculated blood volume calculated from the volume of blood per pump and the volume of the centrifuge, the calculated blood volume per unit operating volume of the liquid pump is calculated, and the blood is collected in the first platelet collection operation. It is preferable to have an extracorporeal circulating blood volume value calculation function of calculating an actual extracorporeal circulating blood volume value using the total operation amount of the liquid feeding pump and the calculated blood supply volume.

(3) In the above (1), the platelet collection device uses the operation amount of the liquid feed pump and the volume of the centrifuge from the start of blood collection to the time when the centrifuge is filled with anticoagulant-added blood. To calculate the calculated blood supply amount per unit operation amount of the liquid supply pump, and use the total operation amount of the liquid supply pump for blood collection in the first platelet collection operation and the calculated blood supply amount to calculate the actual blood supply amount. This is a platelet collection device having an extracorporeal circulating blood volume value calculating function for calculating an extracorporeal circulating blood volume value.

(4) In the above (1), the function of calculating the estimated number of platelets to be corrected by the platelet collection device is to calculate the actual extracorporeal circulating blood volume value in the first platelet collection operation.
The estimated extracorporeal blood volume value calculated from the hematocrit value data, the anticoagulant addition rate, the internal volume of rotation of the centrifuge at the set centrifugal rotation, and the blood throughput when the centrifuge is filled with blood And calculating a correction value of the estimated number of collected platelets using the estimated number of collected platelets.

(5) In any one of the above (1) to (4), the platelet collection device may calculate the corrected number of predicted platelet units calculated from the corrected number of predicted platelets based on the target number of platelet collection units. It is preferable to have a warning function that is activated when it is small.

(6) In any one of the above (1) to (4), the platelet collection device may be arranged so that the corrected collection predicted platelet count calculated by the corrected collection prediction platelet count calculation function or its related value is a predetermined value. It is preferable to have a warning function that is activated when it is smaller.

(7) In any one of the above (1) to (6), the platelet collection device may be arranged so that the corrected collection predicted platelet count calculated by the corrected collection prediction platelet count calculation function or its related value is the target collection platelet count. It is preferable to provide a warning function that is activated when the number of collected platelets or its related value is smaller.

(8) In any one of the above (1) to (7), the platelet collection device includes a centrifuge driving device for rotating the rotor of the centrifuge, a hematocrit value input unit, and a platelet concentration. It is preferable to include an input unit and a target platelet collection number or its related value input unit.

(9) In any one of the above (1) to (8), it is preferable that the platelet collection device has a function of changing the number of platelet collection cycles.

(10) In any one of the above (1) to (9), the liquid sending pump is arranged on a centrifugal separator side from a connection portion between the first line and the first tube, Further, the platelet collection device includes a centrifugal separator driving device for rotating the rotor of the centrifugal separator, a second liquid pump for the third line,
A plurality of flow path opening / closing means for opening / closing the flow path of the platelet collection circuit; the centrifugal separator driving device; the first liquid feeding pump; the second liquid feeding pump; A control device for controlling the means, the control device further comprising: a blood sample to which an anticoagulant is added, a blood sample to be separated, and a plasma sample to be collected in the plasma collection bag. At least one plasma collection / circulation step including a collection step, a plasma circulation step of circulating the plasma in the plasma collection bag collected in the plasma collection step to the centrifuge, and the plasma collection / circulation step After the end of the platelet collection, the plasma circulation speed by the first liquid feed pump is accelerated to cause the platelets to flow out of the centrifuge and collect the platelets in the platelet collection bag. To perform the steps as after completion of the platelet collection step, the platelet collection operation is to perform a blood return step of returning blood to the centrifugal separator of the blood takes place,
It is preferable that the device controls the centrifugal separator driving device, the first liquid feed pump, the second liquid feed pump, and the plurality of flow path opening / closing means.

(11) In any one of the above items (1) to (10), it is preferable that the control device controls the platelet collection operation to be performed at least twice.

[0016]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A platelet collecting apparatus according to the present invention will be described with reference to an embodiment shown in the drawings. FIG. 1 is a plan view showing a configuration example of a platelet collection circuit used in the platelet collection device of the present invention, FIG. 2 is a plan view of a cassette housing part of the platelet collection circuit of FIG. 1, and FIG. FIG. 4 is a partially cutaway cross-sectional view showing a state in which a driving device is mounted on a centrifuge used in a platelet collection circuit. FIG. FIG. 5 is a conceptual diagram, and FIG. 5 is a block diagram of a control device used in the platelet collection device of the present invention.

The platelet collection device 1 of the present invention has a rotor 142 having a blood storage space therein, and an inlet 143 and an outlet 144 communicating with the blood storage space.
The centrifugal separator 20 for centrifuging the blood introduced from the inflow port 143 in the blood storage space by the rotation of 2, the blood collection needle 29 or a blood collection device connection part (not shown), and the inflow port 143 of the centrifuge 20 A first line 21 for connecting;
A second line 22 connected to the outlet 144 of the centrifuge 20, a third line 23 for anticoagulant injection connected to the first line 21, and a halfway point of the first line 21. Connected first tube 25a and second line 2
3 is a platelet collection device for the platelet collection circuit 2 that includes a plasma collection bag 25 having a second tube 25 b connected to the third line 3 and a platelet collection bag 26 connected to the second line 22.

Further, the platelet collection device 1 of the present invention includes a liquid supply pump 11 (first liquid supply pump) provided on the first line 21 and receives a blood donor input before the platelet collection operation. This is a platelet collection device that calculates the number of platelet collection cycles and the predicted number of collected platelets or its related value from the hematocrit value data and platelet concentration data and the target platelet collection number or its related value. Then, after completion of the blood collection in the first platelet collection operation, the platelet collection device of the present invention provides an actual extracorporeal blood volume value in the first platelet collection operation and an estimated extracorporeal blood volume calculated from the hematocrit value data. The apparatus is provided with a function for calculating a corrected expected number of platelets to calculate a correction value of the expected number of collected platelets using the amount value and the estimated number of collected platelets.

The platelet collection device 1 includes a centrifuge drive device 1 for rotating the rotor 142 of the centrifuge 20.
0 and the first pump 11 for the first line 21
And the second liquid supply pump 12 for the third line 23
A plurality of channel opening / closing means 81, 82, 83, 84, 85, 86 for opening and closing the channel of the platelet collection circuit 2;
Centrifuge drive 10, first liquid feed pump 11, second
And a control device 13 for controlling the liquid sending pump 12 and the plurality of flow path opening / closing means. Therefore, the platelet collection circuit 2 will be described first.

The platelet collection circuit 2 is a circuit for collecting platelets. The platelet collection circuit 2 includes a blood collection needle 29
A blood collection device such as, or a connection to a blood collection device having a blood collection needle or blood pool connection (blood collection device connection),
The blood collection needle 29 or the blood collection device connection part is connected to the inflow port 143 of the centrifugal separator 20, and the first pump tube 21g is connected.
A first line 21 (blood collection and return line) comprising:
A second line 22 for connecting the outlet 144 of the centrifuge 20 to the first line 21, which is connected near the blood collection needle 29 of the first line 21, and is connected to the second pump tube 2.
A third line 23 (an anticoagulant injection line) including the first tube 25a and a second line 22 connected to a branch connector 21f located closer to the blood collection needle than the pump tube 21g of the first line 21; A plasma collection bag 25 having a second tube 25b connected thereto,
, A platelet collection bag 26 including a third tube 26 a connected to the second line 22, and a buffy coat collection bag 27 including a fourth tube 27 a connected to the second line 22. The platelet collection circuit 2 may include a connection portion (for example, a metal or synthetic resin needle) for connecting to a blood pool such as a blood bag instead of a blood collection needle.

As the blood collection needle 29, a known metal needle is used. The first line 21 is connected to the blood collection needle side first line 21 a to which the blood collection needle 29 is connected and the inflow port 14 of the centrifuge 20.
3 and the first line 21b connected to the centrifugal separator. The blood collection needle side first line 21a is formed by connecting a plurality of soft resin tubes. The blood collection needle side first line 21a includes a branch connector 21c for connection to the third line 23, a chamber 21d for removing bubbles and microaggregates, and a second line 22 from the blood collection needle side.
Branch connector 21e for connection with the plasma collection bag 25
Branch connector 21f for connection with the first tube 25a
Is provided. An air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d. The centrifuge-side first line 21b is connected to a branch connector 21f for connection to the first tube 25a, and has a pump tube 21g formed in the vicinity thereof.

The second line 22 connecting the outlet 144 of the centrifuge 20 and the first line 21 has one end connected to the outlet 144 of the centrifuge 20 and the other end connected to the first line 21. Is connected to the connection branch connector 21e. The second line 22 is connected to the second tube 25b of the plasma collection bag 25 and the branch connector 22a for connection with the third tube 26a of the platelet collection bag 26 from the centrifugal separator side, and a tube provided with a filter 22f for removing bubbles. A connection branch connector 22c and a branch connector 22d for connection to the fourth tube 27a of the buffy coat collection bag 27 are provided. The third line 23 is a connection branch connector 21 having one end provided on the first line 21.
c. The third line 23 includes, from the connector 21c side, a pump tube 23a, a foreign matter removing filter 23b, a bubble removing chamber 23c, and an anticoagulant container connecting needle 23d.

The plasma collection bag 25 is connected to the first line 21
The first tube 25a and the second tube 25a connected to the branch connector 21f located closer to the blood collection needle than the pump tube 21g.
Connected to the branch connector 22a of the line 22 of FIG.
It has a tube 25b. The platelet collection bag 26 includes a third tube 26a connected to the branch connector 22a of the second line 22. The buffy coat collection bag 27 includes a fourth tube 27a connected to the branch connector 22d of the second line 22. As a constituent material of the tube, the pump tube, and the tube connected to the bag used for forming the above-described first to third lines 21, 22, 23, polyvinyl chloride is preferable. If each tube is made of polyvinyl chloride, sufficient flexibility and flexibility can be obtained, so that it is easy to handle, and it is also suitable for clogging with cleanse or the like. Also, as the constituent material of the branch connector described above, the same material as the constituent material of the tube can be used. As the pump tube, a tube having such a strength that the tube is not damaged even when pressed by a roller pump is used.

Plasma collection bag 25, platelet collection bag 2
6. The buffy coat collection bag 27 is formed by laminating a resin-made flexible sheet material and fusing (thermal fusing, high frequency fusing, etc.) or bonding the peripheral edge thereof into a bag shape. used. As a material used for each of the bags 25, 26, 27, for example, soft polyvinyl chloride is preferably used. As a plasticizer in this soft polyvinyl chloride, for example, di (ethylhexyl) phthalate (D
EHP), di- (n-decyl) phthalate (DnDP)
Etc. are used. Incidentally, the content of such a plasticizer,
The amount is preferably about 30 to 70 parts by weight based on 100 parts by weight of polyvinyl chloride. The platelet collection bag 26
It is more preferable to use a sheet material having excellent gas permeability in order to improve the platelet preservation property. As such a sheet material, for example, using the above-described polyolefin or DnDP plasticized polyvinyl chloride, or the like, without using such a material,
Using a sheet material of the above-described material, the thickness is relatively thin (for example, about 0.1 to 0.5 mm, particularly 0.1 to 0.5 mm).
(Approximately 0.3 mm) is preferable.

The main part of the platelet collection circuit 2 is as follows.
As shown in FIG. 2, it is of a cassette type. The platelet collection circuit 2 includes all lines (first line, second line, and third line) and all tubes (first tube, second tube, third tube, and fourth tube).
Are partially housed and partially hold them, in other words with a cassette housing 28 in which they are partially fixed. Both ends of the first pump tube 21g and both ends of the second pump tube 23a are fixed to the cassette housing 28.
g and 23a project from the cassette housing 28 in a loop shape corresponding to the shape of the roller pump. Therefore, the first and second pump tubes 21g, 23a
Is easy to mount on a roller pump.

Further, the cassette housing 28 has a plurality of openings located in the cassette housing 28. Specifically, the first opening portion 91 exposing the first line 21 on the blood collection needle side from the pump tube 21g and allowing the first flow path opening / closing means 81 of the platelet collection device 1 to enter, The first tube 25a of the collection bag 25 is exposed and the second channel opening / closing means 8 of the platelet collection device 1
Opening 92 through which the second sample 2 can enter, the plasma collection bag 2
5, the third opening 93 through which the third channel opening / closing means 83 of the platelet collection device 1 can enter, and the third tube 26a of the platelet collection bag 26 are exposed, and the platelet collection is performed. Fourth channel opening / closing means 8 of device 1
The fourth opening 94, the second line 22 through which the fourth
And the fourth tube 27a of the buffy coat collection bag 27
A fifth opening 95 that exposes the second line 22 at a position closer to the centrifugal separator (upstream side) than the connection portion with the fifth passage 95 and allows the fifth flow path opening / closing means 85 of the platelet collection device 1 to enter; First
Connection with line 21 and buffy coat collection bag 2
7 exposing the second line 22 between the connection portion of the platelet collection device 1 and the second line 22 (downstream from the connection portion of the second line 22 and the fourth tube 27a). Sixth opening 96 into which channel opening / closing means 86 can enter
It has.

On the inner surface of the cassette housing 28, the above-described branch connector is fixed. Further, near the side surface of the cassette housing 28, a line and a tube protruding from the side surface of the housing are held,
In addition, a reinforcing tube for preventing bending at the housing portion is provided. Cassette housing 28
Is a box-shaped body in which the portion shown by the broken line in FIG. 2 can be stored. Then, the cassette housing 28
Is formed of a synthetic resin having a certain degree of rigidity.

The platelet collection device 1 includes this cassette housing mounting portion (not shown). For this reason, by mounting the cassette housing 28 on the cassette housing mounting portion of the platelet collection device 1, each line and each tube of the portion exposed from the opening of the cassette housing 28 are automatically connected to the corresponding channel opening / closing means. Be attached. Thus, the circuit can be easily mounted, and platelet collection can be quickly prepared. Further, the platelet collection device 1 is provided with two pumps near the cassette housing mounting portion. For this reason, the cassette housing 28
It is also easy to mount the exposed pump tube on the pump.

The centrifuge 20 provided in the platelet collection circuit 2 is usually called a centrifugal bowl, and separates blood components by centrifugal force. As shown in FIG. 3, the centrifugal separator 20 has a vertically extending tube 141 having an inlet 143 formed at an upper end thereof, and rotates around the tube 141 to be liquid-tightly sealed with respect to the upper portion 145. Hollow rotor 142
It is composed of A flow path (blood storage space) is formed in the rotor 142 along the bottom and the inner surface of the peripheral wall, and an outlet 144 is formed to communicate with the upper part of the flow path. In this case, the volume of the rotor 142 is, for example,
It is about 100 to 350 ml.

The rotor 142 is rotated under predetermined centrifugal conditions (rotation speed and rotation time) set by the rotor rotation driving device 10 provided in the platelet collection device 1. According to the centrifugal conditions, a blood separation pattern in the rotor 142 (for example, the number of blood components to be separated) can be set. In this embodiment, as shown in FIG.
Blood flows from the inner layer to the plasma layer 13 in the flow path of the rotor 142.
1. Centrifugation conditions are set so that the buffy coat layer 132 and the red blood cell layer 133 are separated.

Next, the platelet collecting apparatus 1 of the present invention shown in FIG. 4 will be described. The platelet collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid supply pump 11 for the first line 21, and a third liquid supply pump 11 for the third line 23. And a plurality of flow path opening / closing means 81, 82, 83, 84, 85, 8 for opening / closing the flow path of the platelet collection circuit 2.
6, centrifuge drive 10, first liquid pump 1
A control device 13 for controlling the first and second liquid feed pumps 12 and a plurality of flow path opening / closing means is provided. Further, the platelet collection device 1 is attached above the turbidity sensor 14 and the centrifuge 20 which are attached to the second line 22 on the centrifuge side (upstream side) from the connection portion 22a with the second tube 25b. And a weight sensor 16 for detecting the weight of the plasma collection bag 25.

In this embodiment, the platelet collection apparatus calculates the number of platelet collection cycles and the estimated platelet It is a platelet collection device that calculates a number or its related value. For this purpose, the platelet collection device includes a hematocrit value input unit 61, a platelet concentration input unit 62, and a target platelet collection unit number input unit 63 that is a value related to the target platelet collection number.

In the platelet collecting apparatus of the present invention, blood is collected for analysis from a blood donor before the operation of collecting platelets, and the blood for analysis (preliminary blood collected) is further collected using a blood analyzer prepared separately. (Hematocrit value, platelet concentration)
Then, by inputting the blood information data and the target platelet collection number (or target platelet collection unit number) to the platelet collection device, the required number of platelet collection operation cycles is calculated, and the platelet collection operation of that cycle number is performed. I am going to do it.

The control unit 13 includes a control unit 50, a pump controller 53 for the first liquid feed pump 11, and a second
A pump controller 54 for the liquid feed pump 12, an input unit 60, and a display unit 52 as a warning unit. The control unit 50, which is a control mechanism of the control device 13, is electrically connected to the first liquid supply pump 11 and the second liquid supply pump 12 via pump controllers 53 and 54. Further, the operation amount detection unit 5 attached to the first liquid feed pump 11
6 is electrically connected to the control unit 50. As the operation amount detection unit 56, a rotation amount detection unit can be used, and specifically, a rotary encoder can be suitably used. Furthermore, it is also electrically connected to the drive controller 55 provided in the centrifuge drive device (rotor drive device) 10.

The control unit 50 stores necessary data and arithmetic expressions, and further, a memory for storing input hematocrit value data, platelet concentration data, and the like, and performs calculations such as a predicted number of corrected platelets to be collected. Is provided. The input unit 60 of the control device 13 includes a hematocrit value input unit 61, a platelet concentration input unit 62, a target platelet collection unit number input unit 63, a start switch 6
4. A switch 65 for changing the number of platelet collection cycles is provided.

The platelet collection device 1 calculates the number of platelet collection cycles and the estimated number of platelets to be collected or their related values from the hematocrit value data and the platelet concentration data obtained from the blood for analysis and the target platelet collection number or its related value. I do. Note that the relevant value of the target platelet collection number is the target platelet collection unit number. The related value of the expected number of collected platelets is the expected number of platelet units to be collected. The relationship between the number of platelets and the number of platelets is as follows. 20 units of platelets: 4 × 10 ¹¹ or more 15 units of platelets: 3 × 10 ¹¹ or more 10 units of platelets: 2 × 10 ¹¹ or more 5 units of platelets: 1 × 10 ¹¹ or more In terms of the formula, the number of units = 5 × Platelet count / 10 ¹¹
Platelet count = unit number × 2 × 10 ¹⁰

The function of calculating the number of platelet collection cycles and the expected number of collected platelets or its related value from the hematocrit value data and platelet concentration data obtained from the blood for analysis and the target platelet collection number or its related value will be described. In the platelet collection apparatus of this embodiment, specifically, the control unit 50 calculates the number of platelet collection cycles and the number of platelets to be collected or their related values as follows. In this embodiment, the target platelet collection unit number is input, and the calculation unit of the control unit 50 calculates the target platelet collection number. Expected platelet count in the first cycle = extracorporeal blood volume x PLT concentration x predicted recovery rate ...

From the second cycle onward, the blood processing volume in consideration of the extracorporeal blood volume and the residual blood volume inside the bowl and the increase in the platelet count due to the buffy coat recycling performed in the platelet collection apparatus of this embodiment are based on Equation 1. Take into account. Specifically, in the second cycle, the expected number of collected platelets in the second cycle = the blood processing volume in the second cycle × the predicted platelet concentration in the second cycle × the predicted recovery rate + the blood processing volume in the first cycle × the blood volume in the first cycle Measured PLT concentration × Remaining rate × Recycling rate ... Equation 2

Specifically, in the third cycle, the expected number of collected platelets in the third cycle = the blood processing volume in the third cycle × the predicted platelet concentration in the third cycle × the predicted recovery rate + the blood processing volume in the second cycle × the second cycle Predicted platelet concentration at the time × leftover rate × recycling rate + first cycle blood processing volume × predicted PLT concentration at the first cycle × leftover rate × recycling rate ... Equation 3 Thus, 1 cycle, 2, 3 and After the calculation, the cycle number at the time when the target platelet collection number is exceeded is output as the platelet collection cycle number. Further, the number of collected platelets at the end of the cycle is output as the predicted platelet number.

The extracorporeal circulating blood volume in Equation 1 is obtained by the following equation: Extracorporeal circulating blood volume = K × B (%) ÷ HCT (%). In Expressions 1, 2, 3 and 4, K: the volume occupied by red blood cells inside the centrifuge bowl at the end of blood collection (start of platelet collection operation) (200 to 220 ml) B (%): the ratio of red blood cells in K above ( 60 ~
95%) The values of K and B are determined experimentally, and fixed values are used. HCT (%): hematocrit value (hematocrit value obtained from blood collected for analysis)

PLT concentration: platelet concentration (platelet concentration obtained from blood collected for analysis) Predicted recovery rate: as platelet concentrate from the centrifuge container, based on the total number of platelets contained in blood supplied to the centrifuge container in each cycle The percentage of the number of platelets that can be removed to the collection bag is indicated, and empirical values based on experiments are used. Usually 60 to 85%) the target platelet collection stars target platelet collection unit number × 2 × 10 ¹⁰ taken prediction platelet count = collecting anticipated platelet count of the first cycle + the second cycle of the sampling expected number of platelets +. Estimated number of platelets collected in the last cycle: Equation 5

Further, the platelet collection device may calculate the predicted number of platelet collection units. The display unit 52 has a function of displaying the number of platelet collection operation cycles and the estimated number of platelet collections or the estimated number of platelet collection units. Further, the control unit 50 may have a function of calculating an expected platelet collection operation time for calculating an expected end time from the number of platelet collection operation cycles. Further, the control device 13
May have a function of displaying the expected platelet collection operation time.

The platelet collecting apparatus of the present invention
After the blood collection in the first platelet collection operation, specifically, after the first platelet collection operation (in other words, after the end of the first cycle), the actual extracorporeal circulating blood volume value in the first platelet collection operation, A function of calculating a corrected estimated platelet count to calculate a corrected value of the estimated platelet count using the estimated extracorporeal blood volume value calculated from the hematocrit value data (in other words, the input hematocrit value) and the estimated platelet count. I have. The hematocrit value data is the hematocrit value initially input to the platelet collection device, and if there is no input error, the hematocrit value obtained from the blood collected for analysis.

In this platelet collection apparatus, the difference between the predicted blood processing volume and the actual processing volume, that is, whether there is a difference between the input hematocrit value and the actual hematocrit value, Judgment is made after the end, and the blood processing amount at the end of the blood collection in the first platelet collection operation is adopted as (positive), and the already set platelet operation cycle number (first calculated platelet operation cycle number) Is performed to calculate the number of platelets collected (corrected predicted platelet number) and / or the corrected predicted platelet unit number obtained when the above is performed. The display unit 52 displays the estimated number of platelets to be corrected and / or the number of platelet units to be corrected.
It has. Therefore, the operator can know the correction value of the platelet number or platelet unit number obtained after the end of the set platelet collection cycle number, and can cope with a case where the correction value is different from the target value.

The platelet collection device 1 has a function of calculating the calculated blood supply amount per unit operation amount of the liquid supply pump, and further operates the total operation of the liquid supply pump for blood collection in the first platelet collection operation. It is preferable to have an extracorporeal circulating blood volume value calculating function of calculating an actual extracorporeal circulating blood volume value using the volume and the calculated blood supply volume. Here, the extracorporeal circulating blood volume value does not include an anticoagulant.

For example, the platelet collection device calculates the amount of blood pump operation from the start of blood collection to the time the centrifuge is filled with anticoagulant-added blood and the stored amount of blood per unit operation. Using the calculated blood volume and the volume of the centrifuge at the time of the set centrifugal rotation, the calculated blood supply amount per unit operation amount of the liquid transfer pump is calculated, and the liquid transfer for blood collection in the first platelet collection operation is performed. It has an extracorporeal circulating blood volume value calculation function for calculating the actual extracorporeal circulating blood volume value using the total operation amount of the pump and the calculated blood supply volume. To be exact,
The actual extracorporeal blood volume of the anticoagulant-added extracorporeal blood is calculated using the total amount of operation of the liquid supply pump for blood collection and the calculated blood supply volume in the first platelet collection operation. The extracorporeal circulating blood volume value is calculated by subtracting the added anticoagulant amount from the blood volume value.

The calculated amount of blood calculated from the operation amount of the liquid supply pump from the start of blood collection to the time when the centrifuge is filled with the anticoagulant-added blood and the stored blood supply amount per unit operation amount are as follows: The figure shows the blood transfer volume obtained by the operation of the liquid feeding pump from the start of filling the empty centrifuge with the anticoagulant-added blood to the time when the centrifuge is completely filled with the anticoagulant-added blood. If the liquid supply pump is a roller pump as in the illustrated embodiment, the liquid supply amount = the number of rotations of the roller × the liquid supply amount of one rotation of the roller. The roller rotation speed is
The rotation amount is detected by the rotation amount detection unit 56. If the feed pump is a peristaltic pump, the feed rate = pump operation time x
This is the amount of liquid sent per unit time. In the platelet collection device, the platelet collection device stores the amount of liquid sent per rotation of the roller or the amount of liquid sent per unit time.

Then, from the calculated blood volume obtained from the blood volume of the liquid pump and the volume of the centrifuge, the calculated blood volume of the liquid pump is calculated. This is because the platelet collection device memorizes the amount of liquid per rotation of the pump roller (in the case of a roller pump) or the amount of liquid per unit time (in the case of a peristaltic pump) that is different from the actual value. Correction. That is, the known volume of the centrifugal separator at the time of the set centrifugal rotation (the same as the volume at the time of predetermined rotation when the anticoagulant-added blood is filled) is stored in the plasma separation device in advance (specifically, the control unit 50) and this value,
When the blood supply pump is a roller pump, for example, when the liquid supply pump is a roller pump, a comparison is made between the number of rotations of the roller × the liquid supply amount of one rotation of the storage roller, and the actual amount of rotation of the roller is calculated. Calculate the liquid transfer volume. If the liquid sending pump is a peristaltic pump, the actual liquid sending amount per unit time is calculated by comparing the pump operation time × the liquid sending amount per storage unit time with the volume of the centrifuge.

Then, the operation amount of the liquid sending pump (total number of rotations in the case of a roller pump, the operation time in the case of a peristaltic pump) and the calculated blood supply amount per unit operation amount obtained as described above. By calculating the actual extracorporeal circulating blood volume value with the anticoagulant added and subtracting the added anticoagulant amount from the anticoagulant added extracorporeal circulating blood volume value, An actual extracorporeal circulating blood volume value (extracorporeal circulating blood volume) for blood collection is calculated. It should be noted that the platelet collection device may not store the blood supply amount per unit operation amount. In this case, the platelet collection device uses the operation amount of the liquid sending pump and the volume of the centrifugal separator from the start of blood collection to the time when the centrifugal separator is filled with anticoagulant-added blood to operate the unit of the liquid sending pump. Calculate the calculated blood supply volume per volume and calculate the actual extracorporeal circulating blood volume using the total operation volume of the liquid pump for blood collection and the calculated blood flow volume per unit operation volume in the first platelet collection operation. It has an extracorporeal circulating blood volume value calculation function for calculating the value.

In this case, the known volume of the centrifuge at the set centrifugal rotation (the same as the volume of the anticoagulant-added blood filling) is stored in advance in the plasma separating apparatus (specifically, This value is stored in the memory of the unit 50).
Calculated blood transfer per unit time of the liquid sending pump from the amount of operation of the liquid sending pump until the anticoagulant-added blood is filled (roller rotation speed for a roller pump, pump operation time for a peristaltic pump) Calculate the amount. Then, the operation amount of the liquid sending pump (total number of rotations for a roller pump,
In the case of a peristaltic pump, the operation time) and the calculated blood supply amount per unit operation amount obtained as described above,
The actual extracorporeal circulating blood volume value (extracorporeal circulating blood volume) for blood collection in the first platelet collection operation, which is the actual blood transfer volume, is calculated.

The function of the platelet collection device for calculating the corrected expected platelet count is based on the actual extracorporeal blood volume value (RBV) in the first platelet collection operation and the hematocrit value data (in other words, the input hematocrit value). The calculated expected extracorporeal blood volume value (EBV), the anticoagulant addition rate (ACD), the internal volume of rotation of the centrifuge at the set centrifugal rotation (BBV), and the time when the centrifuge is filled with blood It is preferable to calculate a correction value (corrected predicted platelet count) of the predicted predicted platelet count using the blood processing volume (OFV) and the predicted predicted platelet count. In this embodiment, the set centrifugal rotation is 4750 rotations, and the rotation inner volume (BBV) of the centrifuge is 265 m
L (including the amount of ACD-A liquid).
Processing volume = whole blood + ACD-A solution volume = 241 mL + 24 m
L, (ACD-A solution ratio 1/10 is the standard setting). In the platelet collection apparatus of this embodiment, the centrifugal rotation speed was 4750 rpm from the blood collection step to the blood return step.
Is constant.

Then, it is preferable that the corrected platelet count is calculated as follows. Corrected HCT value = αxHCTx (EBV / RBV) x (OFV / BBV) x ((ACD + 1) / ACD) Expression 6 The contents of each symbol in Expression 6 are as follows. EBV: Predicted extracorporeal circulating blood volume (mL) RBV: Extracorporeal circulating blood volume (mL): BBV: Internal volume of rotation of centrifuge bowl at set centrifugal rotation speed (mL) OFV: Blood treatment when centrifuge bowl is filled with blood Volume (mL) HCT: Hematocrit value input (%) ACD: Anticoagulant mixture ratio (shown as whole blood volume per anticoagulant volume 1) α: Coefficient for correction

Then, each term (EBV / RB) in equation (6)
V) is for calculating the error of the blood processing volume, and (O)
(FV / BBV) is for calculating the flow error of the blood pump, and ((ACD + 1) / ACD) is for calculating the whole blood volume in the BBV fluid volume. Using the HCT value calculated by the equation 6, the PLT number is calculated again by the equations 1 to 4, and the corrected PLT number is set as the corrected sampled platelet count. Then, the platelet collection device operates when the corrected collection predicted platelet count calculated by the corrected collection prediction platelet count calculation function or its related value (specifically, the corrected collection predicted platelet unit number) is smaller than a predetermined value. It is preferable to have a warning function.

The warning operation pattern is, for example, a warning pattern that is activated when the corrected expected platelet count calculated by the corrected expected platelet count calculation function is smaller than a predetermined value. The expected number of platelet units to be collected is a predetermined value (minimum number of platelet collection units,
Specifically, it is activated when it is smaller than 2 units),
Any method may be used, such as outputting an alarm when the corrected number of estimated collected platelets calculated from the corrected estimated number of platelets is smaller than the target number of collected platelets (5 × corrected estimated number of collected platelets / 10 ¹¹ ).

Further, in the platelet collecting apparatus of the present invention, it is preferable that the number of platelet collecting operation cycles can be changed after this warning is displayed.
An instruction to change the number of platelet collection operation cycles, specifically, an instruction to increase the number of cycles (usually one cycle) is input (specifically, input from the platelet collection cycle number change switch 65).
Then, it is preferable that the platelet collection device calculates the corrected collection prediction platelet count again. in this case,
It is more preferable to have a function of terminating the operation of the warning function when the set warning condition is not satisfied after the recalculation. Further, it is preferable that the recalculated corrected collection predicted platelet count or its related value (specifically, the recalculated corrected collection predicted platelet unit number) be displayed on the display unit 52.

The platelet collection device may be one in which the number of platelet collection operation cycles is automatically changed after this warning is displayed. In this case, the control unit calculates the number of platelet collection operation cycles required to satisfy the set warning condition and automatically changes the setting. Then, for the reset number of platelet collection cycles, the corrected collection predicted platelet count is calculated again,
It is preferable that the recalculated corrected collection predicted platelet count or its related value (specifically, the recalculated corrected collection predicted platelet unit number) be displayed on the display unit. Further, the platelet collection device includes a manual mode in which the number of cycles is not changed unless the operator changes the number of platelet collection cycles as described above, and an automatic mode when the warning condition is met as described above. It may be provided with an automatic mode in which the number of platelet collection cycles is changed as well as a mode selection function capable of selecting a manual mode or an automatic mode. In this case, the input unit is provided with a mode selection switch.

Although not performed in the platelet collecting apparatus of this embodiment, even after the blood collection is completed in the second and subsequent platelet collecting operations, the corrected predicted platelet count is recalculated to recalculate the correction value of the predicted platelet count. A number recalculation function may be provided. Also, the flow path opening / closing means 81, 82, 8
3, 84, 85, 86 are all connected to the control device,
Their opening and closing are controlled by the control device 13. Further, a turbidity sensor 14, an optical sensor 15 mounted above the centrifuge 20, and a weight sensor 16 for detecting the weight of the plasma collection bag 25 are also electrically connected to the control device 13, and The output signal is input to the control device 13.

The control device 13 has a control mechanism composed of, for example, a microcomputer and a function of calculating the number of rotations of the rotor.
5. The detection signal from the turbidity sensor 14 is input to the control device 13 as needed. The control device 13 is based on signals from the turbidity sensor 14, the optical sensor 15, and the weight sensor 16,
It controls the rotation, stop, and rotation direction (forward / reverse) of each pump, and controls the opening / closing of each flow path opening / closing means and the operation of the centrifuge rotation drive device 10 (rotation of the rotor) as necessary.

The first channel opening / closing means 81 is provided for opening / closing the first line 21 on the blood collection needle side from the pump tube 21g. Second channel opening / closing means 82
Is provided for opening and closing the first tube 25a of the plasma collection bag 25. The third channel opening / closing means 83 includes:
It is provided for opening and closing the second tube 25b of the plasma collection bag 25. The fourth channel opening / closing means 84 is provided for opening / closing the third tube 26 a of the platelet collection bag 26. The fifth flow path opening / closing means 85 is connected to the second line 22 at a position closer to the centrifugal separator (upstream side) than the connection portion 22d between the second line 22 and the fourth tube 27a of the buffy coat collection bag 27. It is provided for opening and closing.

The sixth flow passage opening / closing means 86 is provided between the connecting portion 21e of the first line 21 and the connecting portion of the fourth tube 27a (the connecting portion of the second line 22 and the fourth tube 27a). At a position (downstream side), the second line 22 is provided for opening and closing. The channel opening / closing means includes an insertion portion for a line or a tube.
It has a clamp that operates with a drive source such as a solenoid, electric motor, cylinder (hydraulic or pneumatic). Specifically, a pneumatic cylinder clamp operated by pneumatic pressure is preferable. The clamp of the passage opening / closing means operates based on a signal from the control device 13.

As shown in FIG. 3, the rotor driving device 10 holds a rotor rotation driving device housing 151 accommodating the centrifuge 20, a leg 152, a motor 153 as a driving source, and the centrifuge 20. Disc-shaped fixed base 1
55. The housing 151 includes the leg 1
It is placed and fixed on the upper part of the reference numeral 52. Further, the spacers 15 are provided on the lower surface of the housing 151 by bolts 156.
7, the motor 153 is fixed. Motor 15
A fixed base 155 is fitted to the tip of the third rotating shaft 154 so as to rotate coaxially and integrally with the rotating shaft 154, and the bottom of the rotor 142 is fitted to the upper part of the fixed base 155. A recess is formed. In addition, centrifuge 2
0 is fixed to the housing 151 by a fixing member (not shown). Rotor rotation drive 10
When the motor 153 is driven, the fixed base 155 and the rotor 142 fixed to the fixed base
Spin at 000-6000 rpm.

Further, the rotor rotation driving device housing 1
On the inner wall of 51, an interface of the separated blood components in the centrifuge (for example, the plasma layer 131 and the buffy coat layer 132)
B, buffy coat layer 132 and red blood cell layer 133
Optical sensor 15 for optically detecting the position of
Are installed and fixed by a mounting member 158. As the optical sensor 15, an optical sensor that can scan vertically along the outer peripheral surface of the centrifuge 20 is used. The sensor includes a light source that irradiates light toward the shoulder of the centrifugal separator 20 and a light receiving unit that receives light reflected from the centrifuge bowl and returned. That is, LE
A light-emitting element such as D or laser and a light-receiving element are arranged in a row, and light reflected from a blood component of light emitted from the light-emitting element is received by the light-receiving element, and the amount of received light is photoelectrically converted. Have been.

The separated blood components (for example, plasma layer 13
1 and the buffy coat layer 132), the position of the interface corresponding to the light receiving element where the amount of received light has changed is detected as the position of the interface B. More specifically,
Based on the difference in the amount of light received by the light receiving portion between when the light passing through the centrifuge 20 is filled with a transparent liquid (plasma or water) and when it is filled with the buffy coat layer, the buffy coat It is detected that the layer has reached the light passage. The position at which the buffy coat layer is detected is adjusted by changing the position at which light passes through the bowl. Usually, once the light passing position is determined, it is fixed there.

The turbidity sensor 14 is for detecting the turbidity of the fluid flowing in the second line 22, and outputs a voltage value corresponding to the turbidity. Specifically, a low voltage value is output when the turbidity is high, and a high voltage value is output when the turbidity is low. The first liquid supply pump 11 to which the pump tube 21g of the first line 21 is attached and the second liquid supply pump 12 to which the pump tube 23a of the third line 23 is attached are roller pumps, peristaltic pumps. Non-blood contact type pumps such as are preferred. As the first liquid sending pump 11 (blood pump), a pump capable of sending blood in any direction is used. Specifically, a roller pump capable of normal rotation and reverse rotation is used.

The control device collects the blood to which the anticoagulant has been added, separates the collected blood, and collects the separated plasma in a plasma collection bag. At least one plasma collection / circulation step comprising a plasma circulation step of circulating plasma in a plasma collection bag through a centrifuge; and, after the plasma collection / circulation step, after the plasma collection / circulation step, a plasma circulation rate by a first liquid feeding pump Accelerate the, platelet collection step of causing the platelets to flow out of the centrifuge and collect the platelets in the platelet collection bag, and after the platelet collection step,
A blood return step of returning blood in the centrifuge is performed.

Specifically, the control device 13 operates the first liquid supply pump 11 and the second liquid supply pump 12 to collect the blood to which the anticoagulant has been added, and the centrifuge drive device 10
Is operated (by rotating the rotor at the above-mentioned calculated value or set value) to perform a first plasma collection step of collecting a first predetermined amount of plasma from the blood into the plasma collection bag 25, Next, the blood collection is temporarily interrupted, and the centrifuge driving device 10 is operated (by rotating the rotor at the above-described calculated value or set value) to remove the plasma in the plasma collection bag from the centrifugal separator 20. A constant-speed plasma circulation step (constant-speed circulation) to circulate at a constant speed, and perform a plasma collection / constant-speed circulation step. Next, the first liquid-feed pump 11 and the second liquid-feed pump 12 are operated. When activated, blood with anticoagulant added thereto is collected and the centrifuge drive 10
Operating (rotating the rotor with the above-mentioned calculated value or set value), and collecting a plasma until a predetermined position (for example, a buffy coat layer) is detected by the interface sensor; After the completion of the second plasma collection step, the blood collection is temporarily interrupted, and the centrifuge driving device 10 is operated (by rotating the rotor at the above-mentioned calculated value or set value) to thereby remove the plasma collection bag 25.
A plasma collection / acceleration circulation step comprising an accelerated plasma circulation step (accelerated circulation) for circulating the plasma in the centrifuge 20 while accelerating the plasma therein, and after the plasma collection / circulation step, the first liquid feed pump 11 A platelet collection step of accelerating the plasma circulation speed of the platelet to cause the platelets to flow out of the centrifuge 20 and collect the platelets into a platelet collection bag, and after the platelet collection step is completed, returning the blood in the centrifuge 20 To perform a blood return step. In the second plasma collection step, the weight of the plasma bag is not detected because the detection is performed by the interface sensor.

Thus, during one platelet collection operation,
The plasma recirculation step of suspending blood collection and recirculating the collected plasma to the centrifuge is performed at least twice, and the latter half of the plasma recirculation step is accelerated circulation, so that the In this case, the blood cell layer and the buffy coat layer (BC layer) can be prevented from being excessively compressed, and platelets buried in the red blood cell layer can be lifted up and taken into the BC layer. Further, since the BC layer itself also rises, the separation and alignment of platelets and white blood cells in the BC layer are promoted. For this reason,
It is possible to obtain a platelet-containing liquid (rich platelet plasma) with little leukocyte contamination and high platelet collection efficiency. Further, the controller 13 of the platelet collection device 1 of this embodiment
Is the above mentioned plasma collection and constant speed circulation step,
The centrifugal separator driving device 10, the first liquid feed pump 11, the second liquid feed pump 12, and the plurality of flow paths are set so that the platelet collection operation including the accelerated circulation step, the platelet collection step, and the blood return step is performed twice. It controls the road opening / closing means.

Further, the control device 13 of the platelet collection apparatus 1 of this embodiment
Before the blood return step, the plasma circulation speed by the first liquid feeding pump 11 is set higher than the final speed in the platelet collection step, the buffy coat is discharged from the centrifuge 20, and the buffy coat is collected in the buffy coat collection bag 27. To perform a buffy coat collection step. The buffy coat collecting step is not limited to the above-described method. For example, the plasma circulation speed by the first liquid feeding pump 11 is maintained at the final speed in the platelet collecting step, and the centrifugal separator 20 This may be performed by lowering the rotation speed of the rotor. Further, the buffy coat collecting step may be performed by making the plasma circulation speed by the first liquid feeding pump higher than the final speed in the platelet collecting step and lowering the rotation speed of the rotor of the centrifuge.

After the buffy coat collection step is completed, the first liquid feeding pump is operated so that a buffy coat return step of returning the collected buffy coat to the centrifuge 20 before the next blood collection step is performed. 11 and a plurality of channel opening / closing means.

The operation of collecting platelets will be specifically described with reference to the flowcharts shown in FIGS. As shown in FIG. 6, first, the operator uses the sampled blood collected from the donor just before starting the component blood collection,
Perform blood cell component measurement in advance. The hematocrit value (HCT) and platelet concentration (PLT)
Concentration) Further, the target number of platelet collection units is input to the apparatus.
The control unit of the platelet collection device calculates the target minimum platelet collection value, the estimated extracorporeal blood volume, the platelet collection operation cycle number, and the predicted platelet collection number using the input values and the stored data or calculation formula. I do. Further, it may calculate the expected number of platelet collection units. Then, the number of platelet collection operation cycles and the predicted platelet collection number or the predicted platelet collection unit number are displayed on the display unit 52. Further, the control unit may have a function of calculating an expected end time from the number of platelet collection operation cycles and displaying the calculated end time.

The estimated number of collected platelets is calculated by the following equation (5). Estimated number of collected platelets = Estimated number of collected platelets in the first cycle + Estimated number of collected platelets in the second cycle + Number of platelets And Equation 1 below
Thus, the expected number of platelets to be collected in the second cycle is calculated by the following equation 2, and the expected number of platelets to be collected in the third cycle is calculated by the following equation 3. Expected platelet count in the first cycle = extracorporeal blood volume x PLT concentration x predicted recovery rate ...

Expected number of collected platelets in the second cycle = second cycle blood throughput × predicted platelet concentration in the second cycle × estimated recovery rate + first cycle blood throughput × predicted PLT concentration in the first cycle × remaining Rate x Recycle Rate Equation 2 Expected number of platelets collected in the third cycle = Blood processing volume in the third cycle x Predicted platelet concentration in the third cycle x Predicted recovery rate + Blood processing volume in the second cycle x Second cycle Predicted platelet concentration × remaining rate × recycling rate + first cycle blood throughput × predicted PLT concentration in first cycle × remaining rate × recycling rate Equation 3 And the number of final cycles is 1 cycle, 2, It is calculated as 3, and is calculated as the number of cycles when the number of collected platelets exceeds the target number.

The extracorporeal circulating blood volume in Equation 1 is
It is calculated by the following equation 4. Extracorporeal circulating blood volume = K × B (%) ÷ HCT (%) Expression 4 In this embodiment, K is the volume occupied by red blood cells in the centrifuge bowl at the end of blood collection (start of platelet collection operation). Yes, specifically 200-230ml, B (%)
Is the ratio of red blood cells in the above K,
95%. Note that HCT (%) is a hematocrit value input value, and PLT concentration is a platelet concentration input value. The predicted recovery rate is 65 to 80%. The target number of platelets to be collected is calculated from the target number of platelet collection units × 2 × 10 ¹⁰ . And the third line 2
3 and the blood collection needle 29 are primed with an anticoagulant, and then the donor is punctured with a puncture needle to start collecting blood components.

An anticoagulant is prescribed for whole blood (1 for whole blood)
/ 8 to 1/20, specifically 1/10) at a predetermined speed (250 ml / min or less; preferably 150 ml / min or less).
４０40 ml / min or less, specifically, 60 ml / mi
n) through the first line 21 to the centrifugal separator 20 to rotate the centrifugal separator 20 at a predetermined rotational speed [3000 to 600].
0 rpm, preferably in the range of 4400 to 5800 rpm and automatically calculated by using the hematocrit value of the donor (initial rotor rotation speed)] to convert blood into plasma, buffy coat, and erythrocyte components. Separation, when the plasma overflows the centrifuge 20, the blood is collected in a plasma bag, and when a predetermined amount of plasma (10 to 150 ml, preferably 20 to 30 ml) has been collected, the blood transfer is stopped.
The plasma was subjected to a predetermined condition (at a rate greater than the
10 to 90 sec at ~ 250 ml / min, specifically, the first circulation is 200 ml / min x 30 sec)
A constant-rate plasma circulation is performed, returning to the centrifuge 20 through the first line 21 and the second line 22.

Then, an anticoagulant is again added to whole blood (1/8 to 1/20, specifically 1/10 with respect to whole blood).
At a predetermined speed (250 ml / min or less; preferably 150 to 40 ml / min or less, specifically,
(60 ml / min or less) to the centrifugal separator 20 via the first line 21 to rotate the centrifugal separator 20 at a predetermined rotation speed (3
000-6000 rpm, preferably 4700-48
(00 rpm) to separate blood into plasma, buffy coat, and red blood cell components. When the blood cell interface position inside the centrifuge 20 is detected by the buffy coat interface detection sensor, the blood transmission is stopped. Under predetermined conditions (initial speed 60 ~
80 ml / min, final arrival speed (set speed) 150 to
250 ml / min, acceleration conditions (every second) 2 to 10
ml / min speed increase, circulation time 10-90 sec)
Then, accelerated plasma circulation is performed by returning to the centrifuge 20 through the first line 21 and the second line 22.

Then, under predetermined conditions (blood collection amount: 100 to 25)
00 / Hct% [ml], specifically, 250 to 100
(0 / Hct% [ml]), a small amount of whole blood is collected while adding an anticoagulant again. After the last blood draw,
The plasma is returned to the centrifuge 20 through the first and second lines 22 under predetermined conditions, and the acceleration is gradually increased under predetermined conditions (stepwise acceleration; 0.1 to 99 ml).
/ Min / sec, specifically 2 to 10 ml / min
/ Sec) Platelet collection speed (60-250 ml / mi)
n; actually, 200 ml / min), and the platelets that have flowed out from the centrifuge 20 are collected in the platelet collection bag 2.
6 to be collected.

Further, in this apparatus, after collecting the platelets, the blood circulation speed is maintained (60 to 250 ml / min, specifically 200 ml / min), and the centrifuge 20
Lower the number of rotations (100 to 30
By lowering the buffy coat by about 0 rpm), the buffy coat that has flowed out is collected, and the collected buffy coat is supplied to the centrifugal separator 20 before performing blood collection in the next cycle. The buffy coat may be collected by accelerating the blood circulation speed to a predetermined speed (equal to or higher than the platelet collection speed, preferably 60 to 250 ml / min, specifically 205 ml / min) after collecting the platelets. Good.

In this embodiment, the platelet collection operation is repeated a plurality of times, and after the platelet collection steps other than the last one are completed, before the blood return step, a buffy coat collection step is performed, and the next blood collection step is performed. Before this, a buffy coat return step of returning the buffy coat to the centrifugal separator 20 is performed. Specifically, after blood collection starts,
As shown in FIG. 6, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect blood to which the anticoagulant is added, and the centrifugal separator driving device 10 is operated to collect the blood. A first plasma collection step of collecting a first predetermined amount of plasma into the plasma collection bag 25 is performed.

When the first blood collection is started, the blood pump 1
1 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is simultaneously driven at a predetermined speed (for example, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.
Blood collected from the donor is mixed with ACD fluid and
, Flows through the chamber, the first channel opening / closing means 81, and flows into the centrifuge 20. At this time,
The sixth flow path opening / closing means 86, the fifth flow path opening / closing means 85, the second flow path opening / closing means 82, and the third flow path opening / closing means 83 are closed, and the first flow path opening / closing means 81, The fourth channel opening / closing means 84 is open. When the ACD blood is supplied to the centrifuge 20, the sterilized air that has entered the centrifuge 20 flows through the second line 22, passes through the fourth channel opening / closing means 84, and enters the platelet collection bag 26. Flows into.

Simultaneously with the start of the blood collection process, the centrifuge 20 starts rotating at a predetermined speed, and the centrifuge 20 receives the supply of ACD blood while rotating. The blood is separated from the inside into a plasma layer, a buffy coat layer (BC layer), and an erythrocyte layer. The blood is completely filled and the blood flows out of the outlet of the centrifuge 20. The turbidity sensor 14 attached to the second line 22 connected to the outlet of the centrifuge 20 detects that the fluid flowing in the line has changed from air to plasma, and
3 closes the fourth flow path opening / closing means 84 based on the detection signal of the turbidity sensor 14 and sets the third flow path opening / closing means 8
3 is opened, and the plasma is collected in the plasma collection bag 25.

The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control device 13. For this reason, the weight of the plasma collected in the plasma collection bag 25 is the first predetermined amount (10 to 10).
150 g, for example, 30 g).
Closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a constant-speed plasma circulation step. In the constant-velocity plasma circulation step, blood collection is suspended,
At the same time, the centrifuge driving device 10 is operated to circulate the plasma collected in the plasma collection bag 25 to the centrifuge 20 at a constant speed.

In the constant-velocity plasma circulation step, the control device 13 sets the closed state of the first flow path opening / closing means 81 and the second state.
, The ACD pump 12 stops, and the blood pump 11 operates at a predetermined speed (60 to 25).
0 ml / min, for example, 200 ml / min), and the plasma in the plasma collection bag 25 is
2 to a centrifugal separator 20 that rotates at a predetermined speed (calculated value of the initial rotor speed). At the same time, the plasma flowing out of the centrifuge 20 flows into the plasma collection bag 25 through the turbidity sensor 14 and the third channel opening / closing means 83. After the constant-speed plasma circulation step has started,
After 90 seconds (for example, 30 seconds) elapse, the control device 13
Closes the second flow path opening / closing means 82, opens the first flow path opening / closing means 81, and shifts to a second plasma collection step.
The first plasma circulation is preferably performed at a flow rate of at least 60 ml / min or more for 10 seconds or more.

In the second plasma collection step, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant has been added, and usually the amount of plasma in the bag is reduced. Due to the increase, when the optical sensor 15 detects the buffy coat layer of the separator, this signal is sent to the control device 13, and the control device 13 closes the first flow path opening / closing means 81, and closes the second flow path. The road opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. Specifically, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the second pump, which is an anticoagulant pump, is simultaneously driven at a predetermined speed (for example, 1/1 of the blood pump speed).
At 0), an anticoagulant (for example, ACD-A solution) is supplied.

The blood collected from the donor is mixed with the ACD solution, flows into the centrifugal separator 20 rotating at a predetermined speed (calculated value of the initial rotor speed), and the plasma is collected in the plasma collection bag 2.
Collect within 5. Normally, when the optical sensor 15 detects the buffy coat layer of the separator due to an increase in the amount of plasma in the bag, this signal is sent to the control device 13 and the control device 13 Is closed, the second flow path opening / closing means 82 is opened, and the process proceeds to the accelerated plasma circulation step. In the second plasma collection step, the sensor 15
Until the Buffy coat (BC interface: interface between the plasma layer and the buffy coat layer) is detected.

In the accelerated plasma circulation step, the blood collection is temporarily interrupted, and the centrifuge driving device 10 is operated to circulate the plasma in the plasma collection bag 25 to the centrifuge 20 while accelerating. At this time, the blood pump speed is lower than the constant-speed plasma circulation step, for example, 60 ml / mi.
n and the final speed is 150-200ml / mi
Accelerate until n is reached. The acceleration conditions are as follows: a speed of 2 to 10 ml / min increases every second, 200 m
It is performed with an arrival time of 1 / min about 14 to 70 seconds. After the end of the circulation step, the process proceeds to FIG. 7, and a small amount plasma collection step for adjusting the interface is performed.

As shown in FIG. 7, in the step of collecting a small amount of plasma for adjusting the interface, in order to keep the position of the buffy coat layer constant in the subsequent platelet collecting step regardless of the donor,
Blood is collected for a predetermined supply amount of red blood cells. The red blood cell supply amount is defined as a value obtained by dividing the blood collection amount by the hematocrit value of the donor, and the blood collection amount is generally about 12 ml. Also in this blood collection, the first liquid supply pump 11 starts blood collection at a predetermined speed (for example, 60 ml / min). At this time,
The second pump, which is an anticoagulant pump, simultaneously supplies an anticoagulant (for example, ACD-A liquid) at a predetermined rate (for example, 1/10 of the blood pump speed). The blood collected from the donor is mixed with the ACD solution and flows into the centrifuge 20 rotating at a predetermined speed (calculated value of the initial rotor speed).
A small plasma collection is performed. The control device 13 calculates the collection time from the set collection amount and the pump speed, and ends the blood collection when the collection time has elapsed. And the control device 13
Closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82, and shifts to a platelet collection step.

After the above steps are completed, the plasma circulation speed of the first liquid feed pump 11 is increased, and the centrifuge 20
Platelets are drained from the inside and platelets are collected in a platelet collection bag 26.
A platelet collection step of collecting platelets. The platelet collection step is also called a so-called acceleration process. In this step, the blood pump speed is 60 ml /
The control device 13 operates the blood pump to accelerate 200 ml / min from 10 min / min to 200 ml / min by 10 ml / min every predetermined time (for example, 1 second).
min, until the platelet collection process is over,
Maintain that speed.

When the platelet collection step is started, the turbidity sensor 14 detects the turbidity of the liquid passing therethrough, and the turbidity is output as a voltage value by the sensor, and the output signal is input to the control device 13. When the speed of the blood pump increases and reaches approximately 160 to 200 ml / min, platelets contained in the buffy coat layer remaining in the centrifuge 20 flow out. Turbidity sensor 14 when platelets flow out
When the turbidity of the liquid passing through the portion increases and the voltage value output from the sensor decreases by 0.2 V, the third flow path opening / closing means 83 closes, the fourth flow path opening / closing means 84 opens, and The platelet-rich plasma flowing out of the separator 20 is collected in the platelet collection bag 26. The voltage value output from the turbidity sensor 14 is converted into a platelet concentration by the control device 13 to calculate the platelet concentration in the platelet collection bag 26 during platelet collection. The platelet concentration in the platelet collection bag 26 once reaches the maximum concentration and then decreases. At the point in time when it is detected that the maximum concentration has been reached, the platelet collection step ends, and the process proceeds to the buffy coat collection step.

In the buffy coat collecting step, when the above-described platelet collecting step is completed, the controller 13 closes the fourth flow path opening / closing means 84 and the fifth flow path opening / closing means 85
Release. The plasma in the plasma collection bag 25 is sent to the centrifugal separator 20 by the blood pump 11, and at the same time, the liquid (the buffy coat layer has flowed out) flowing out of the centrifuge 20 flows into the buffy coat collection bag 27. . In the buffy coat collection step, the blood pump 11
Is maintained at the final speed in the platelet collection step, and the rotational speed of the centrifuge 20 is a predetermined speed or slightly higher than the predetermined speed (from 50 to over the predetermined speed).
(About 200 rpm, for example, 100 rpm higher rotation speed)
Then, the buffy coat flows out of the centrifuge 20 and is collected in the buffy coat collection bag 27. When the amount calculated from the hematocrit value of the donor and the amount of collected platelets is collected, the blood pump 11 is stopped, all valves are closed, the rotation of the centrifuge 20 is stopped, and the buffy coat collection step is completed.

Next, a blood return step of returning the blood in the centrifuge 20 is performed. The control device 13 controls the blood pump 11
Is rotated in the reverse direction, the first channel opening / closing means 81 is opened, and the red blood cell layer remaining in the centrifuge 20 is returned to the donor via the first line 21. Thus, the first (first) platelet collection operation is completed.

Subsequently, the flow shifts to the second platelet collection operation shown in FIG. Before shifting to the second platelet collection operation, a corrected predicted platelet count is calculated. After completion of the first cycle, the platelet collection device of the present invention provides the following: Using the number, a corrected predicted platelet count, which is a correction value of the predicted platelet count, is calculated. The calculation of the corrected sampled platelet count may be performed not after the completion of the first cycle but after the calculation becomes possible in the first cycle. Specifically, after the blood collection in the first cycle is completed, in this embodiment, the calculation can be performed if the blood collection is completed in the small-volume plasma collection step.

The calculation of the corrected estimated platelet count is performed as follows. Corrected HCT value = αxHCTx (EBV / RBV) × (OFV / BBV) × ((ACD + 1) / ACD) Expression 6 The contents of each symbol in Expression 6 are as follows. EBV: Predicted extracorporeal circulating blood volume (mL) RBV: Extracorporeal circulating blood volume (mL): BBV: Internal volume of rotation of centrifuge bowl at set centrifugal rotation speed (mL) OFV: Blood treatment when centrifuge bowl is filled with blood Volume (mL) HCT: Input hematocrit value (%) ACD: Anticoagulant mixture ratio (shown as whole blood volume relative to anticoagulant volume 1) α: Coefficient for correction The HCT value calculated by equation 6 Then, the number of PLTs is calculated again according to Equations 1 to 4, and is used as the corrected estimated platelet count.

[0093] The platelet collection device stores the amount of blood sent per unit operation amount of the liquid sending pump, specifically, the amount of blood sent per rotation of the roller pump. Since an accurate extracorporeal circulating blood volume cannot be obtained, an accurate extracorporeal circulating blood volume is obtained as follows. Specifically, in the platelet collection device 1, the operation amount of the liquid sending pump 11 from the start of blood collection to the time when the centrifugal separator is filled with anticoagulant-added blood is determined by the operation amount detection unit (rotation amount detection unit) 5.
6, the known volume of the centrifugal separator is divided by the operation amount (rotation amount) of the liquid supply pump detected by the control unit, whereby accurate delivery per unit operation amount of the liquid supply pump is achieved. The blood volume (calculated blood volume) is calculated, and this calculated blood volume is used thereafter. Then, based on the signal output from the operation amount detection unit (rotation amount detection unit) 56, the control unit 50 calculates the operation amount of the liquid feeding pump 11 in the total blood collection time until the last blood collection in the first cycle. Then, an accurate extracorporeal circulating blood volume is calculated from the calculated total pumping blood pumping volume and the calculated blood pumping volume per unit operating volume of the liquid feeding pump.

Then, according to the above equation 6, the estimated number of collected platelets due to the difference between the input value of the hematocrit value and the actual hematocrit value is corrected, output as the corrected estimated number of collected platelets, and displayed. Further, in the platelet collecting apparatus of this embodiment, as shown in FIG. 8, the corrected predicted platelet count is a predetermined value (specifically, 1.94 × 10 ¹¹ ).
If smaller, a warning is displayed on the display unit. Further, the lamp 57 may blink. 1.95
If it is not less than × 10 ¹¹ , it is permissible as 10 unit platelets, and if it is intended to collect 10 units of platelets, 1.94 × 10 ¹¹ is used as the predetermined value. Then, the operator who sees this warning can change the number of platelet collection operation cycles. The number of platelet collection operation cycles is changed by the platelet collection cycle number change switch 65. For example, when the number of platelet collection operation cycles initially set by calculation is three, the cycle number is changed to four.

When the number of platelet collection operation cycles is changed, the corrected number of predicted platelets is recalculated, and the corrected number of collected platelets after the recalculation is set to a predetermined value (specifically, 1.94).
× 10 ¹¹ ), the warning on the display section disappears. The lamp 57 is also turned off. If the expected end time is displayed, it is calculated again and displayed.
Then, when a warning is displayed on the display unit, the normal operator changes the cycle number setting and cancels the warning display. However, after a predetermined time (for example, after 30 seconds), the cycle is automatically set. The number may be incremented by one to cancel the alarm display.

If no warning is displayed on the display, the process immediately proceeds to the second cycle. Although not shown in the flowchart of FIG. 8, after the warning is displayed on the display unit, the number of platelet collection operation cycles can be changed at any time, and the correction is performed immediately when the number of platelet collection operation cycles is changed. The estimated number of collected platelets is recalculated, and the corrected estimated number of collected platelets after the recalculation is a predetermined value (specifically, 1.94).
In the case of × 10 ¹¹ ) or more, the warning on the display section disappears.
The lamp 57 is also turned off. If the expected end time is displayed, it is calculated again and displayed.

The warning operation pattern is not limited to the above-described predetermined value. The warning indicates that the corrected collection predicted platelet unit number calculated from the corrected collection predicted platelet count calculated by the corrected collection predicted platelet count calculation function is:
A predetermined value (for example, the minimum number of platelet collection units, specifically,
(2 units). Further, as shown in the flowchart of FIG. 12, the warning may be activated when the corrected number of predicted platelet units calculated by the corrected number of collected platelets unit is smaller than the target number of collected platelets.

In the second cycle, first, as shown in FIG. 8, the buffy coat collected by the first platelet collection operation is centrifuged before the next plasma collection step.
A buffy coat return step for returning within is performed.
When the process proceeds to the buffy coat return step, the control device 1
3 rotates the centrifugal separator 20 at a predetermined rotation speed (initial rotor rotation speed calculation value),
Is opened, and the blood pump 11 is operated at a predetermined speed (the default is 100 ml / min).
The buffy coat contained in the buffy coat collection bag 27 passes through the fifth flow path opening / closing means 85 and passes through the centrifugal separator 2.
0 is supplied. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 is rotated by the amount of the buffy coat collected, the buffy coat return step ends.

Subsequently, as shown in FIG. 8, a first plasma collection step, a constant-speed plasma circulation step, a second plasma collection step, and an accelerated plasma circulation step are performed. The plasma collection step, the platelet collection step, the buffy coat collection step, and the blood return step are sequentially performed, and the second platelet collection operation is completed.

Next, the final platelet collection operation shown in FIG. 10 will be described. In this embodiment, the third time is the final time, but the present invention is not limited to this, and the fourth time and thereafter may be the final time of the platelet collection operation. in this case,
Except for the last round, it is the same as the second platelet collection operation (FIGS. 8 and 9).

In the final platelet collection operation, first, as shown in FIG. 10, the buffy coat collected by the second (previous) platelet collection operation is centrifuged by a centrifugal separator 20 before the next plasma collection step. Perform the buffy court return step to return within. When the process proceeds to the buffy coat return step, the control device 13 rotates the centrifugal separator 20 at a predetermined rotation speed (for example, 4800 rpm) to open the fifth channel opening / closing unit 85 and the fourth channel opening / closing unit 84. , The blood pump 11 at a predetermined speed (default is 100 ml / mi)
Operate in n). The buffy coat contained in the buffy coat collection bag 27 is supplied to the centrifugal separator 20 through the fifth channel opening / closing means 85. The air in the centrifuge 20 is sent to the platelet collection bag 26 through the second line 22 and the fourth channel opening / closing means 84. After the blood pump 11 is rotated by the amount of the buffy coat collected, the buffy coat return step ends.

Next, the first liquid supply pump 11 and the second liquid supply pump 12 are operated to collect the blood to which the anticoagulant is added, and the centrifugal separator driving device 10 is operated to remove the blood. A first plasma collection step of collecting a first predetermined amount of plasma in the plasma collection bag 25 is performed. When the first blood collection is started, the first liquid supply pump 11 operates at a predetermined speed (for example, 60
(ml / min). At this time, the second pump, which is an anticoagulant pump, also has a predetermined speed (for example,
An anticoagulant (eg, AC) at 1/10 of the blood pump speed
(DA solution). The blood collected from the donor is A
The mixture is mixed with the CD solution, flows through the first line 21, passes through the chamber, the first channel opening / closing means 81, and
Flows into.

At this time, the sixth channel opening / closing means 86 and the fifth
Are closed, the second channel opening / closing unit 82, and the third channel opening / closing unit 83 are closed.
The first and fifth flow path opening / closing means 85 are open, and when the ACD blood is supplied to the centrifuge 20, the centrifuge 2
The sterilized air that has entered 0 flows into the buffy coat collection bag 27 through the line sensor and the fifth flow path opening / closing means 85. Simultaneously with the start of the blood collection process, the centrifuge 20 starts rotating at a predetermined speed.
Since the blood supply D is supplied, the blood is centrifuged in the separator, and the blood is separated from the inside into three layers of a plasma layer, a buffy coat layer (BC layer), and a red blood cell layer. Is supplied, blood in the centrifugal separator 20 is completely filled, and plasma flows out from the outlet of the centrifugal separator 20.

The second outlet connected to the outlet of the centrifuge 20
The turbidity sensor 14 attached to the line 22 detects that the fluid flowing in the line has changed from air to plasma, and the control device 13 performs the fifth operation based on the detection signal of the turbidity sensor 14. With the flow path opening / closing means 85 closed and the third flow path opening / closing means 83 opened, the plasma is collected in the plasma collection bag 25. The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control device 13. For this reason,
When the weight of the plasma collected in the plasma collection bag 25 increases by a predetermined amount (for example, 30 g), the control device 13 closes the first flow path opening / closing means 81 and opens the second flow path opening / closing means 82. Then, move to the constant-speed plasma circulation step.

Then, the above-mentioned constant-speed plasma circulation step,
The second plasma collection step and the accelerated plasma circulation step are performed, and the process proceeds to FIG. 11, where a small-volume plasma collection step for interfacial adjustment, a platelet collection step, and a blood return step are sequentially performed, and the final platelet collection operation is completed. I do. Final round and 2
The difference from the first time is that the bag in which air flows in the above-mentioned constant-speed plasma circulation step is different, and in the last time, the blood return step is performed without performing the buffy coat collection step.

[0106]

According to the platelet collection apparatus of the present invention, the number of platelet collection cycles and the predicted platelet collection number are obtained from the donor's hematocrit value data and platelet concentration data and the target platelet collection number or a related value input before the platelet collection operation. In the platelet collection device that calculates the number or the related value, after the blood collection in the first platelet collection operation is completed, the actual extracorporeal circulating blood volume value in the first platelet collection operation and the prediction calculated from the hematocrit value data The apparatus is provided with a function for calculating a corrected expected platelet count based on the extracorporeal circulation blood volume value and the estimated predicted platelet count. For this reason, the operator
It is possible to confirm a corrected expected number of collected platelets more accurately than the expected number of collected platelets in the expected number of platelet operation cycles during the platelet collection operation. Then, the operator can appropriately cope with a case where the corrected estimated collection platelet count is smaller than the estimated collection platelet count.

[Brief description of the drawings]

FIG. 1 is a plan view showing a configuration example of a platelet collection circuit used in a platelet collection device of the present invention.

FIG. 2 is a plan view of a cassette housing part of the platelet collection circuit of FIG. 1;

FIG. 3 is a partially cutaway sectional view showing a state in which a driving device is mounted on a centrifuge used in a platelet collection circuit.

FIG. 4 is a conceptual diagram of one embodiment of the platelet collection device of the present invention with a platelet collection circuit mounted.

FIG. 5 is a block diagram of a control device used in the platelet collection device of the present invention.

FIG. 6 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 7 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 8 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 9 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 10 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 11 is a flowchart for explaining the operation of the platelet collection device of the present invention.

FIG. 12 is a flowchart for explaining the operation of the platelet collection device of the present invention.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 platelet collection device 2 platelet collection circuit 10 centrifuge drive device 11 first liquid feed pump 12 second liquid feed pump 13 controller 14 turbidity sensor 15 optical sensor 16 weight sensor 20 centrifuge 21 first Line 22 Second line 23 Third line 25 Plasma collection bag 26 Platelet collection bag 29 Blood collection needle

Claims (11)

[Claims] A rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and rotating the rotor to centrifuge blood introduced from the inlet into the blood storage space. A centrifuge for separation, a first line for connecting a blood collection needle or a blood collection device connection part to an inlet of the centrifuge, and a second line connected to the outlet of the centrifuge A third line connected to the first line for injecting an anticoagulant; a second tube connected to the first line and the second line connected in the middle of the first line; A platelet collection circuit comprising a plasma collection bag having a tube, a platelet collection bag connected to the second line, and a liquid sending pump provided in the first line, and input before the platelet collection operation Donated blood A platelet collection apparatus that calculates the number of platelet collection cycles and the number of platelets to be collected or its related value from the hematocrit value data and platelet concentration data of the subject and the target platelet collection number or its related value, After the blood collection in the first platelet collection operation is completed, the actual extracorporeal circulation blood volume value in the first platelet collection operation, the predicted extracorporeal circulation blood volume value calculated from the hematocrit value data, and the collection predicted platelet count are used. A platelet collection device having a correction collection platelet count calculation function for calculating a correction value of the collection platelet count. 2. The blood collection apparatus according to claim 1, wherein the platelet collection device calculates the amount of operation of the liquid supply pump from the start of blood collection to the time when the anticoagulant-added blood is filled in the centrifuge and the stored amount of blood per unit operation amount. Using the calculated blood volume and the volume of the centrifuge, the calculated blood supply volume per unit operation amount of the liquid supply pump is calculated, and the total operation of the liquid supply pump for blood collection in the first platelet collection operation The platelet collection device according to claim 1, further comprising an extracorporeal circulating blood volume value calculating function for calculating an actual extracorporeal circulating blood volume value using the volume and the calculated blood supply volume. 3. The unit for pumping the platelet using the operation amount of the pump and the volume of the centrifuge from the start of blood collection until the centrifuge is filled with anticoagulant-added blood. Calculate the calculated blood supply amount per operation amount, and calculate the actual extracorporeal circulating blood volume value using the total operation amount of the liquid supply pump for blood collection in the first platelet collection operation and the calculated blood supply amount. 2. The platelet collection device according to claim 1, further comprising an extracorporeal circulating blood volume value calculating function. 4. The function of calculating a corrected expected platelet count of the platelet collection device according to the present invention includes a function of calculating an actual extracorporeal blood volume in the first platelet collection operation and an expected extracorporeal blood volume calculated from the hematocrit value data. Using the anticoagulant addition rate, the internal volume of rotation of the centrifuge at the set centrifugal rotation, the blood throughput when the centrifuge is filled with blood, and the estimated platelet collection, 2. The platelet collection device according to claim 1, wherein the platelet collection device calculates a correction value of the number. 5. The platelet collection apparatus according to claim 1, further comprising a warning function that is activated when the corrected number of predicted platelet units calculated from the corrected number of predicted platelets is smaller than the target number of platelet collection units. 5. The apparatus for collecting platelets according to any one of claims 4 to 4. 6. The platelet collection device according to claim 1, further comprising a warning function that is activated when the corrected collection predicted platelet count calculated by the corrected collection prediction platelet count calculation function or its related value is smaller than a predetermined value. 5. The platelet collection device according to any one of 1 to 4. 7. The alarm which is activated when the corrected expected platelet count or its related value calculated by the corrected expected platelet count calculating function is smaller than the target platelet collection number or its related value. The platelet collection device according to any one of claims 1 to 6, having a function. 8. The centrifuge driving device for rotating the rotor of the centrifuge, wherein the platelet collection device includes:
The platelet collection device according to any one of claims 1 to 7, further comprising a hematocrit value input unit, a platelet concentration input unit, a target platelet collection number or a related value input unit. 9. The platelet collection device according to claim 1, wherein the platelet collection device has a platelet collection cycle number changing function. 10. The liquid sending pump is disposed on a centrifugal separator side from a connection portion between the first line and the first tube, and further, the platelet collection device is provided in the centrifugal separator. A centrifugal separator driving device for rotating the rotor, a second liquid feed pump for the third line, and a plurality of channel opening / closing means for opening and closing the channel of the platelet collection circuit; The centrifuge drive, the first
A liquid supply pump, a control device for controlling the second liquid supply pump and the plurality of flow path opening / closing means, and further, the control device collects and collects blood to which an anticoagulant is added. Plasma collection step of collecting the separated blood and the separated plasma into the plasma collection bag, and a plasma circulation step of circulating the plasma in the plasma collection bag collected by the plasma collection step to the centrifuge. At least one plasma collection / circulation step comprising: after the plasma collection / circulation step, increasing the plasma circulation speed by the first liquid feeding pump to cause platelets to flow out of the centrifugal separator, In the platelet collection bag, and after the platelet collection step, performing a blood return step of returning blood in the centrifuge. 2. The apparatus according to claim 1, wherein the centrifugal separator driving device, the first liquid supply pump, the second liquid supply pump, and the plurality of flow path opening / closing means are controlled so that a platelet collection operation is performed. 10. The apparatus for collecting platelets according to any one of claims 9 to 9. 11. The platelet collection device according to claim 10, wherein the control device controls the platelet collection operation to be performed at least twice.