JP2002272836A - Blood component collecting device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood component collecting device capable of reducing the time for collecting blood components by reducing a blood treatment quantity. SOLUTION: The blood component collecting device performs a blood component collecting process for collecting the blood components (blood plasma) by centrifuging blood collected from a donor when collecting the blood components (blood plasma), and a blood component returning process for returning the residual blood components to the donor. In the blood component returning process, a target returning quantity W is calculated (step S203), and whether or not a returned quantity V amounts to the target returning quantity W is judged (step S205). When it amounts to the target returning quantity W, the blood component returning process is finished and the process is shifted to the blood component collecting process.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component collecting apparatus for collecting a predetermined blood component from blood.

[0002]

2. Description of the Related Art At the time of blood collection, at present, blood is separated into various blood components by centrifugation or the like for reasons such as effective use of blood and reduction of the burden on donors, and only components necessary for the transfuser are collected. Blood is collected from components collected and returned to donors.

[0003] In such a component blood sampling, for example, when obtaining a plasma product, blood collected from a donor is introduced into a blood component collecting circuit, and the blood is collected by a centrifugal separator called a centrifugal bowl installed in the blood component collecting circuit. , Blood plasma, leukocytes, platelets, and red blood cells, and the plasma is collected in a container to form a plasma product, and the remaining white blood cells, platelets, and red blood cells are returned to the donor. Will be

By the way, in such a blood component collecting apparatus for collecting component blood, for example, in the case of collecting plasma, a blood component collecting step of collecting plasma and providing a blood donor with remaining blood components (blood components other than plasma) are performed. And a blood component return step of returning the blood component is performed alternately.

[0005] The blood component collecting step and the blood component returning step are repeatedly performed a plurality of times. Thus, the total amount of plasma collected in each blood component collection step reaches the amount of plasma to be collected from the donor (donor), that is, the target amount of plasma to be collected (target amount of plasma).

However, in the blood component return step, all the remaining blood components in the blood component collection circuit are returned, so that it takes time to collect plasma. As a result, the donor is restrained for a long time, and the occupancy time of the bed is extended.

[0007] Furthermore, the amount of whole blood to be processed required for plasma collection is increased, which places a great burden on donors.

[0008]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a blood component collecting apparatus capable of reducing the amount of processed blood and reducing the time required for collecting the blood components.

[0009]

This and other objects are achieved by the present invention which is defined below as (1) to (12).

(1) A blood collecting means for collecting blood from a donor and a rotor having a blood storage space therein, and a centrifuge for centrifuging the blood collected by the blood collecting means in the blood storage space by rotation of the rotor. A blood component collection circuit that includes a separator and a blood component collection bag that collects a predetermined blood component separated by the centrifuge, and centrifugally separates blood collected from a donor; A blood component collection device for returning the remaining blood components to the blood donor after collecting the blood components, detecting means for detecting the amount of the predetermined blood component collected from the blood donor, and collecting the blood components from the blood donor. The remaining blood component to be returned to the donor based on the target collection amount of the predetermined blood component and the current collection amount of the predetermined blood component detected by the detection means. Calculation means for calculating the return amount of the target of, using the information obtained by the calculation means,
A blood component collection device configured to return the remaining blood components to the donor.

(2) A blood collecting means for collecting blood from a blood donor, and a rotor having a blood storage space therein, and a centrifuge for centrifuging the blood collected by the blood collecting means in the blood storage space by rotation of the rotor. A blood component collection circuit that includes a separator and a blood component collection bag that collects a predetermined blood component separated by the centrifuge, and centrifugally separates blood collected from a donor; A blood component collecting step of alternately repeating a blood component collecting step of collecting the blood component and a blood component returning step of returning the remaining blood components to the blood donor, wherein the predetermined blood component collected from the blood donor is Detecting means for detecting an amount of collected blood; a target amount of the predetermined blood component to be collected from the blood donor; and a current sampling of the predetermined blood component detected by the detecting means. Calculating means for calculating a target return amount of the remaining blood component to be returned to the blood donor based on the amount, and using the information obtained by the calculating means, A blood component collecting apparatus, which is configured to return a blood component.

(3) A blood component collecting apparatus for separating blood collected from a donor into a plurality of blood components, collecting predetermined blood components, and returning the remaining blood components to the donor. A rotor having a blood storage space, an inlet and an outlet communicating with the blood storage space, and centrifuging blood introduced from the inlet by rotation of the rotor into a plurality of blood components in the blood storage space. Centrifugal separator, blood collection means for collecting blood from the donor, a first line connecting the inflow port and the blood collection means, a blood component collection bag for collecting the predetermined blood component, A second line connecting an outlet and the blood component collection bag, detection means for detecting an amount of the predetermined blood component collected from the blood donor, and the predetermined blood component collected from the blood donor Calculating means for calculating a target return amount of the remaining blood component to be returned to the blood donor based on the target collection amount of the target and the current collection amount of the predetermined blood component detected by the detection means. And a device configured to return the remaining blood components to the blood donor using information obtained by the calculation means.

(4) A blood component collecting step of separating blood collected from a donor into a plurality of blood components and collecting a predetermined blood component, and a blood component returning step of returning the remaining blood components to the donor. A blood component collection device that alternately and repeatedly performs, a rotor having a blood storage space therein, an inlet and an outlet communicating with the blood storage space, and introduced from the inlet by rotation of the rotor. A centrifuge for centrifuging blood into a plurality of blood components in the blood storage space, a blood collection unit for collecting blood from the donor, a first line connecting the inflow port and the blood collection unit,
A blood component collection bag for collecting the predetermined blood component,
A second connecting the outlet and the blood component collection bag;
Line, detection means for detecting the collection amount of the predetermined blood component collected from the blood donor, target collection amount of the predetermined blood component collected from the blood donor, detected by the detection means Calculating means for calculating a target return amount of the remaining blood component to be returned to the blood donor, based on the current collection amount of the predetermined blood component, and information obtained by the calculation means. A blood component collection device configured to return the remaining blood component to the blood donor.

[0014] (5) The current amount of the predetermined blood component detected by the detection means after the predetermined blood component is collected and before the remaining blood component is returned to the donor. The blood component collection device according to any one of the above (1) to (4), which is a collection amount.

(6) The target return amount is a value obtained by multiplying a difference between the target collection amount and the current collection amount by a predetermined coefficient, and is one of the above (1) to (5). The blood component sampling device according to claim 1.

(7) The blood component collecting apparatus according to any one of (1) to (6), wherein the detecting means is a weight sensor.

(8) The blood component collection device according to (7), wherein the weight sensor measures the weight of the predetermined blood component collected in the blood component collection bag together with the blood component collection bag.

(9) When returning the remaining blood components to the donor, when the return of the remaining blood components to the donor is completed without reaching the target return amount, The blood component collection apparatus according to any one of (1) to (8), wherein the return of the blood component to the donor is completed.

(10) When returning the remaining blood components to the donor, when the return amount of the remaining blood components to the donor reaches the target return amount, the remaining blood components are returned to the donor. The blood component collection device according to any one of (1) to (9), wherein the return of the blood component to the donor is completed.

(11) The method according to any one of (1) to (10), wherein when the current collection amount reaches the target collection amount, all of the remaining blood components are returned to the blood donor. Blood component collection device.

(12) The blood component collecting apparatus according to any one of (1) to (11), wherein the predetermined blood component is mainly plasma.

[0022]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a blood component collecting apparatus according to the present invention will be described in detail with reference to the preferred embodiments shown in the accompanying drawings.

FIG. 1 is a plan view showing an example of the configuration of a blood component collecting apparatus according to the present invention, and FIG. 2 is a view showing a cross section of a centrifuge used in the blood component collecting apparatus according to the present invention.

As shown in these figures, the blood component collecting apparatus 1 is provided with a blood collecting needle (blood collecting means) for collecting blood from a donor.
A centrifuge (centrifuge bowl) 20 for centrifuging the blood collected by the blood collection means 29 in the blood storage space by rotation of the rotor 142, and a centrifuge 20; And a plasma collection bag (blood component collection bag) 25 for collecting plasma (predetermined blood components) separated by the method described above, and centrifugally separates blood collected from a donor. Then, it is a plasma collection device (blood component collection circuit) that collects plasma (predetermined blood components) and returns the remaining blood components (mainly white blood cells, platelets, and red blood cells) to the donor.

The blood component collection device 1 includes a weight center (detection means) 16 for detecting a collection amount of a predetermined blood component (plasma) collected from a donor, and a blood center (detection means) for collecting plasma (predetermined blood component) from the donor. Based on the target collection amount and the collection amount of plasma (predetermined blood component) detected by the detection means, the return amount of the remaining blood components (white blood cells, platelets, and red blood cells) to be returned to the donor is calculated. Calculating means,
The information obtained by the calculation means is used to return the remaining blood components (white blood cells, platelets, and red blood cells) to the donor.

In this embodiment, an example in which the blood component collection device 1 is applied to a plasma collection device will be described. In the present invention, the blood component collection device 1 is a blood component collection device that collects blood components other than plasma. It can also be applied as a device, for example, a platelet collection device.

Hereinafter, blood components other than plasma returned to the donor, that is, leukocytes, platelets, and erythrocytes will be described as “remaining blood components”.

The blood component collecting apparatus 1 has a blood component collecting circuit 2, and the blood component collecting circuit 2 includes a rotor 142 having a blood storage space therein, and an inflow port 14 communicating with the blood storage space.
And a centrifuge 20 for centrifuging the blood introduced from the inlet 143 by rotation of the rotor 142 in the blood storage space, and a blood collection needle 29 and a centrifuge 20 as blood collection means. A first line 21 for connecting the inlet 143 and an outlet 144 of the centrifuge 20;
A second line 22 connected to the first line 21; a third line 23 connected to the first line 21 for anticoagulant injection;
A plasma collection bag 25 connected to the second line 22;
It has a plasma collection bag 25 and a sub-bag 32 connected by a tube 32a.

The blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid feed pump 11 for the first line 21, A second liquid supply pump 12 for the third line 23, a first flow path opening / closing means 51 and a second flow path opening / closing means 52 for opening and closing the flow path of the blood component collection circuit.
Centrifuge drive device 10, first liquid feed pump 11,
The control unit 5 for controlling the second liquid sending pump 12, the first flow path opening / closing means 51, and the second flow path opening / closing means 52
5 is provided.

As the blood collection needle 29, for example, a metal needle is used. The first line 21 is disposed between the blood collection needle-side first line 21a to which the blood collection needle 29 is connected and the centrifuge-side first line 21b to which the inflow port 143 of the centrifuge 20 is connected. It comprises the first pump tube 21g.

The first line 21a on the blood collection needle side has a chamber 21d for removing bubbles and microaggregates. An air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d.

The second line 22 has one end connected to the centrifugal separator 2.
0 and the other end is connected to the plasma collection bag 2
5 is connected.

One end of the third line 23 is connected to a connection branch connector 21c provided on the first line 21. The third line 23 includes a second pump tube 23a, a bubble removal chamber 23c, and an anticoagulant container connection needle 23d from the connection branch connector 21c side.

The constituent materials of the tubes used for forming the first line 21, the second line 22, and the third line 23, the pump tube, and the tubes connected to the bag are polychlorinated. Vinyl is preferred.

If each tube is made of polyvinyl chloride,
This is because sufficient flexibility and flexibility can be obtained, so that it is easy to handle, and it is also suitable for blocking by cleansing or the like.

Also, as the constituent material of the above-mentioned branch connector, the same constituent material as that of the tube can be used. As the pump tube, a tube having such a strength as not to be damaged even when pressed by a roller pump is used.

The plasma collection bag 25 and the sub-bag 32
In each case, a resin-made flexible sheet material is layered, and the peripheral portion thereof is fused (heat fusion, high-frequency fusion or the like) or bonded to form a bag.

As a material used for the plasma collection bag 25 and the subbag 32, for example, soft polyvinyl chloride is preferably used. Examples of the plasticizer in the soft polyvinyl chloride include di (ethylhexyl) phthalate (DEHP) and di- (n-decyl) phthalate (Dn).
DP) or the like is used. The content of such a plasticizer is 30 to 70 parts by weight based on 100 parts by weight of polyvinyl chloride.
It is preferable to use about parts by weight.

The main part of the blood component collecting circuit 2 is of a cassette type as shown in FIG. The blood component collection circuit 2 is connected to all the lines (the first line 2
1, a second line 22 and a third line 23) are partially housed and partially hold them, in other words comprise a cassette housing 33 in which they are partially fixed.

Both ends of the first pump tube 21g and both ends of the second pump tube 23a are fixed to the cassette housing 33.
g and 23a project from the cassette housing 33 in a loop shape corresponding to the shape of the roller pump. Therefore, the first pump tube 21g and the second pump tube 23a can be easily mounted on the roller pump.

Further, the cassette housing 33 has a plurality of openings located in the cassette housing 33. More specifically, the first line 21 on the blood collection needle 29 side is exposed from the first pump tube 21g, and the first flow path opening / closing means 51 of the blood component collection device 1 can enter the first line 21. An opening is provided with a second opening that exposes the second line 22 and allows the second flow path opening / closing means 52 of the blood component collection device 1 to enter.

The blood component collecting apparatus 1 includes the cassette housing mounting section (not shown). For this reason, by mounting the cassette housing 33 on the cassette housing mounting portion of the blood component collection apparatus 1, each line and each tube of the portion exposed from the opening of the cassette housing 33 automatically correspond to the corresponding channel opening / closing means. Attached to. Thus, the circuit can be easily mounted, and the preparation for collecting blood components can be quickly performed.

The blood component collecting apparatus 1 is provided with two pumps near the cassette housing mounting portion. Therefore, the pump tube exposed from the cassette housing 33 can be easily mounted on the pump. The centrifuge 20 provided in the blood component collection circuit is usually
It is called a centrifugal bowl and separates blood components by centrifugal force. As the centrifuge 20, the one shown in FIG. 2 is used.

Further, the blood component collection device 1 includes a turbidity sensor 14 mounted on the second line 22 and a centrifuge 20
The optical sensor 15 attached above the sensor detects the weight of the plasma collection bag 25 (the weight of the plasma collection bag 25 and the plasma collected in the plasma collection bag 25) (weighs).
(Weight sensor) 16 for detecting
The first line 21 on the first liquid sending pump 11 side from the position d.
The first bubble sensor 17 provided above and the chamber 2
A second bubble sensor 18 provided on the first line 21 on the blood collection needle 29 side with respect to 1d.

The first channel opening / closing means 51 is provided for opening / closing the first line 21 on the blood collection needle side from the pump tube 21g.

The second channel opening / closing means 52 is provided for opening / closing the second line 22. Since the second line is closed by the roller pump when the second line is stopped, the second flow path opening / closing means 52 does not always need to be provided.

Each of the first channel opening / closing means 51 and the second channel opening / closing means 52 has an insertion portion for a line or a tube. The insertion portion includes, for example, a solenoid, an electric motor,
It has a clamp that operates with a drive source such as a cylinder (hydraulic or pneumatic). Specifically, an electromagnetic clamp operated by a solenoid is preferable. The clamps of the first channel opening / closing unit 51 and the second channel opening / closing unit 52 operate based on a signal from the control unit 55.

As shown in FIG. 2, the centrifuge driving device 10 includes a centrifuge rotation driving device housing 151 for accommodating the centrifuge 20, a leg 152, a driving source motor 153, and a centrifugal separator. And a disk-shaped fixed base 155 for holding the container 20. The centrifuge rotation drive housing 151 is mounted and fixed on the upper part of the leg 152.

On the lower surface of the centrifuge rotation driving device housing 151, the spacers 15 are fixed by bolts 156.
7, a motor 153 is fixed. Motor 15
A fixed base 155 is fitted to the tip of the third rotating shaft 154 so as to rotate coaxially and integrally with the rotating shaft 154, and the bottom of the rotor 142 is fitted to the upper part of the fixed base 155. A recess is formed.

The upper part 145 of the centrifuge 20 is fixed to the centrifuge rotation drive housing 151 by a fixing member (not shown). In the centrifuge rotation driving device 10, when the motor 153 is driven, the fixed base 155 and the rotor 142 fixed thereto are rotated under predetermined centrifugal conditions (for example, rotation speeds of 1000 to 6).
000 rpm). According to the centrifugation conditions, a blood separation pattern in the rotor 142 (for example, the number of blood components to be separated) can be set.

The interface of the separated blood components in the centrifuge (for example, the plasma layer 131 and the buffy coat layer 13) is provided on the inner wall of the centrifuge rotation drive housing 151.
2, the buffy coat layer 132 and the red blood cell layer 13
An optical sensor 15 for optically detecting the position of the interface with the interface 3 and the interface between the plasma layer and the blood cell layer) is installed and fixed by a mounting member 158.

This sensor comprises a light source for irradiating light toward the shoulder of the centrifuge 20 and a light receiving unit for receiving light reflected from the centrifuge bowl and returned. In other words, a light-emitting element such as an LED or a laser and a light-receiving element are arranged in a row, and light reflected by a blood component of light emitted from the light-emitting element is received by the light-receiving element, and the amount of received light is photoelectrically converted. Is configured. Separated blood components (for example, plasma layer 131 and buffy coat layer 13)
Since the intensity of the reflected light differs according to 2), the position corresponding to the light receiving element in which the received light amount has changed is detected as the position of the interface B.

More specifically, the light-receiving portions of the centrifuge 20 when the light passes therethrough are filled with a transparent liquid (plasma or water) and filled with a buffy coat layer. It is detected from the difference in the amount of received light that the buffy coat layer has reached the light passing portion.

The position at which the buffy coat layer is detected is adjusted by changing the position at which light passes through the bowl. Usually, once the light passing position is determined, it is fixed there.

The turbidity sensor 14 is for detecting the turbidity of the fluid flowing in the second line 22, and outputs a voltage value corresponding to the turbidity. Specifically, a low voltage value is output when the turbidity is high, and a high voltage value is output when the turbidity is low.

The first bubble sensor 17 and the second bubble sensor 18 are for detecting that air has flowed in the first line 21. As the turbidity sensor and the bubble sensor, an ultrasonic sensor, an optical sensor, an infrared sensor, and the like can be used.

The first liquid supply pump 11 to which the first pump tube 21g of the first line 21 is attached and the third liquid supply pump 11
As the second liquid feed pump 12 to which the second pump tube 23a of the line 23 is attached, a non-blood contact type pump such as a roller pump is suitable. As the first liquid sending pump 11 (blood pump), a pump capable of sending blood in any direction is used. Specifically, a roller pump capable of normal rotation and reverse rotation is used.

The control unit 55 performs control so as to collect blood from a blood donor (donor) and to collect plasma from the blood components (blood components). Specifically, the blood collected by the blood collection needle 29 and added with the anticoagulant is centrifuged by the centrifugal separator 2.
And a blood component collecting step of collecting the plasma separated by the centrifugal separator into the plasma collection bag 25 and blood components other than the plasma remaining in the centrifugal separator 20 (remaining blood components). And performing a blood component return step of returning the blood component.

In the blood component collection step, plasma is collected from the donor until the target amount of plasma to be collected from the donor, that is, the target collection amount (target plasma collection amount) is reached.

In the blood component collection step, the amount of blood collected in the plasma collection circuit 2, that is, the extracorporeal blood circulation volume of the donor is the allowable extracorporeal circulation volume (for example, 15
%), Or when the turbidity sensor 14 detects a buffy coat, the blood component collection step is terminated.

If the amount (collected amount) of plasma collected in the blood component collecting step has not reached the target collected amount, after the blood component returning step to be performed next is completed, the blood component collecting step is performed again. Perform the process and collect plasma from the donor.

That is, in the blood component collecting apparatus 1, the blood component collecting step and the blood component returning step are alternately and repeatedly performed until the target collection amount is reached. Thereby, the total amount of plasma collected in each blood component collection step reaches the target collection amount, and the collection of plasma ends.

The operation principle of the blood component collection step and the blood component return step in the collection of plasma will be briefly described below.

As a premise for starting the collection of plasma, first,
The third line 23 and the blood collection needle 29 are primed with an anticoagulant, and then the donor is punctured with a puncture needle.

[Blood Component Collection Step] When collecting plasma from a blood donor, first, the first liquid supply pump 11 starts collecting blood at a predetermined speed (for example, 60 ml / min). At this time, the second liquid supply pump 12 which is an anticoagulant pump also simultaneously supplies an anticoagulant (for example, ACD-A liquid) at a predetermined speed (for example, 1/10 of the speed of the first liquid supply pump 11). Supply.

Blood collected from a donor is mixed with ACD-A solution, and a mixture of the blood and ACD-A solution (AC
D-added blood) flows through the first line 21 and the chamber 21
d, first channel opening / closing means 51, first pump tube 2
After passing through 1 g, it flows into the centrifuge 20. At this time,
First flow path opening / closing means 51 and second flow path opening / closing means 52
Is open.

When the ACD blood is supplied to the centrifuge 20, the sterilized air entering the centrifuge 20 flows through the second line 22, passes through the second channel opening / closing means 52, and collects plasma. It flows into the bag 25.

At the same time as the start of the blood component collection step,
The rotor 142 of the centrifuge 20 is driven at a predetermined speed (for example,
At 4800 rpm), the centrifugal separator 20 is supplied with ACD-added blood while rotating, and the centrifugal separator 20 performs centrifugal separation of the blood. This allows blood to
Plasma layer 131, buffy coat layer (BC layer) 1 from inside
32, and is separated into three layers of a red blood cell layer 133.

Then, the capacity of the centrifuge 20 (for example,
If more than about 265 ml) of ACD blood is supplied,
The inside of the centrifuge 20 is completely filled with blood, and the plasma flows out of the outlet 144 of the centrifuge 20.

The outflowing plasma is collected in the plasma collection bag 25. Here, the weight of the plasma collected in the plasma collection bag 25 is measured by the weight sensor 16 together with the plasma collection bag 25. Then, the weight sensor 16 transmits a weight signal indicating the weight to the control unit 55, and the control unit 55 receives the weight signal to thereby obtain information on the weight of the plasma collection bag 25 containing the plasma. The weight of the plasma, that is, the amount of the collected plasma is constantly measured from the acquired and acquired information and the previously input weight of the plasma collection bag 25 itself.

When the amount of collected plasma reaches the target amount, the rotation of the rotor 142 and the rotation of the first liquid supply pump 11 are stopped, the second channel opening / closing means 52 is closed, and the blood The component collecting step ends.

If the turbidity sensor 14 detects a buffy coat before the amount of collected plasma reaches the target amount, the rotation of the rotor 142 and the rotation of the first liquid supply pump 11 are stopped. Then, the second channel opening / closing means 52 is closed, and this blood component collecting step is completed.

If the extracorporeal blood circulation amount of the donor reaches the permissible extracorporeal circulation amount before the turbidity sensor 14 detects the buffy coat, the rotation of the rotor 142 and the first
Of the liquid sending pump 11 is stopped, the second channel opening / closing means 52 is closed, and the blood component collecting step is ended.

[Blood Component Returning Step] After the blood component returning step is completed, in the next blood component collecting step, if the amount of plasma to be collected is small, it is necessary to return all remaining blood components in the centrifuge bowl 20. There is no.
For this reason, in this blood component collection device 1, in this blood component return step, the return amount of the remaining blood components to be returned to the donor, that is, the target return amount, is calculated each time, and the blood supply is performed using the information. Return the remaining blood components to the person.

When returning the remaining blood components to the donor,
First, the target return amount is calculated. The return amount for this goal is
Target collection amount, current collection amount (current collection amount),
That is, after the plasma is collected (after the blood component collection step is completed), the weight sensor (detection means) 16 weighs (detects) the weight of the plasma before returning the remaining blood components to the blood donor. Is calculated by the control unit (calculation means) 55 based on

Specifically, the target return amount W is a value obtained by multiplying a difference between the target collection amount X and the current collection amount Y by a predetermined coefficient Z. That is, it is obtained from the following equation (1).

W = (X−Y) × Z (1) (where W = return amount of target, X = target collection amount, Y = current collection amount, Z = coefficient)

Here, the coefficient Z is not particularly limited.
The value is preferably about 1.4 to 4, and more preferably about 2.

The coefficient Z may be constant irrespective of the donor, and before starting the collection of the blood from the donor, the coefficient Z is determined based on the hematocrit value of the donor's blood measured in advance. May be set to the coefficient Z.

Then, the return of the remaining blood components in the centrifuge 20 is started based on the calculated target return amount W.

In this blood component return step, the centrifuge 2
0 stops the rotation, the first flow path opening / closing means 51 and the second flow path opening / closing means 52 are in an open state, and in this state, the first liquid supply pump 11 rotates reversely (for example, 45 ml / min). . Thus, the remaining blood components in the centrifuge 20 pass through the centrifuge first line 21b and the blood collection needle side first line 21a, and are returned to the blood donor from the blood collection needle 29.

Here, the return amount V of the remaining blood component returned to the blood donor is constantly calculated by the control unit 55 based on the rotation speed of the first liquid feeding pump 11.

The control unit 55 constantly calculates the return amount V and determines whether the return amount V has reached the target return amount W. When the return amount V reaches the target return amount W, the operation of [2] described below is performed, and the blood component return step is completed.

When the return amount V does not reach the target return amount W, that is, before the return amount V reaches the target return amount W, the remaining blood components in the centrifuge bowl 20 are returned to the donor. When the remaining blood components in the centrifugal bowl 20 become empty, the following operation is performed.

[1] When the return amount V does not reach the target return amount W and the remaining blood components in the centrifugal bowl 20 become empty, the return amount V does not reach the target return amount W, and the centrifugation is performed. When the remaining blood components in the bowl 20 are empty,
When the remaining blood components have been returned to the blood donor without reaching the target return amount W, the first air bubble sensor 17 attached to the blood collection needle side first line 21a detects the air in the tube. The existence is confirmed.

At this time, in order to return the remaining blood components in the chamber 21d from the first bubble sensor 17 to the blood donor, the speed of the first liquid supply pump 11 is reduced by one step (for example, 30 ml / min). Then, the first liquid supply pump 11 is rotated a predetermined number of times (for example, 10 times).

As a result, almost all of the remaining blood components in the blood component collection circuit 2 are returned to the donor, the blood component return process ends, and the next blood component collection process starts.

[2] When the Return Volume V Reaches the Target Return Volume W When the return volume V reaches the target return volume W, the first liquid feed pump 11 is stopped and the blood component return process is performed. Terminate.

When the blood component return step is completed, the remaining blood component that has not been returned remains in the centrifugal bowl 20, and in that state, the next blood component collection step is performed.

That is, in the blood component return step, not all of the remaining blood components in the centrifugal bowl 20 are returned, but the remaining blood components corresponding to the target return amount W are returned to the blood donor. A predetermined amount of the remaining blood components remains in the centrifugal bowl 20. Therefore, in the next blood component collecting step, the volume of the centrifugal bowl 20 is filled with a small amount of collected blood. Thus, the time required for returning the remaining blood components in the blood component return step and the time required for collecting blood in the subsequent blood component collection step can be reduced.

In this way, the blood component collection step and the blood component return step are performed alternately and repeatedly, and the amount of collected plasma (current collection amount Y) reaches the target collection amount X.

When the current collection amount Y reaches the target collection amount X, the following blood component return step is performed.

[3] When the current collection amount Y reaches the target collection amount X When the current collection amount Y reaches the target collection amount X, the remaining blood components in the blood component collection circuit 2 are removed. Return all to the donor.

More specifically, when the first liquid feed pump 11 is rotated in the reverse direction (for example, 45 ml / min) and the first bubble sensor 17 detects air in the tube, the first liquid feed pump 11 Reduce the rotation speed by one step (for example, 30 ml /
min), the first liquid feed pump 11 is turned on a predetermined number of times (for example, 1
0 times).

Thus, all the remaining blood components in the blood component collection circuit 2 are returned to the blood donor, and the blood component return step ends. It should be noted that the collection of plasma is completed by the completion of the blood component return step.

Next, the control operation of the controller 55 in the blood component collection step and the blood component return step in the collection of plasma will be described more specifically.

FIG. 3 shows the control unit 5 in the blood component collecting step.
FIG. 4 is a flowchart showing the control operation of the control unit 55 in the blood component return step. Hereinafter, description will be made based on this flowchart.

[Blood Component Collection Step] As shown in FIG. 3, in the blood component collection step, first, the first
(For example, 60 ml / min)
To collect blood from the donor (step S10).
1). And, as described above, the collected blood is A
The mixture is mixed with the CD-A solution, and flows into the centrifugal bowl 20.

Next, the rotor 142 is rotated (for example, 4
800 rpm) (step S102). During the rotation, the plasma flows out from the outlet 144 of the centrifuge 20 while the ACD blood is flowing into the centrifuge bowl 20.

The plasma that has flowed out is collected in the plasma collection bag 25 and measured by the weight sensor 16. As described above, the control unit 55 measures the amount of collected plasma from the weight signal acquired from the weight sensor 16.

Next, it is determined whether or not the collected amount of plasma has reached the target collected amount X (step S103). If it has, the rotation of the rotor 142 and the rotation of the first liquid supply pump 11 are determined. Stops, the second flow path opening / closing means 52 closes (step S106), and the blood component collecting step ends. Thereby, the process proceeds to the next blood component return step.

If the plasma collection amount has not reached the target collection amount X, it is next determined whether or not the extracorporeal circulation amount of the donor has reached the allowable extracorporeal circulation amount (step S10).
4) If it has arrived, the rotation of the rotor 142 and the rotation of the first liquid feed pump 11 are stopped, the second flow path opening / closing means 52 is closed (step S106), and this blood component collecting step is ended. . Thereby, the process proceeds to the next blood component return step.

If the extracorporeal circulation amount of the donor has not reached the allowable extracorporeal circulation amount, it is next determined whether or not the turbidity sensor 14 has detected a buffy coat (step S10).
5) If the boundary is detected, the rotation of the rotor 142 and the rotation of the first liquid supply pump 11 are stopped, the second flow path opening / closing means 52 is closed (Step S106), and the blood component collecting step is completed. I do. Thereby, the process proceeds to the next blood component return step.

If no buffy coat has been detected, the process returns to step S102, and steps S102 and thereafter are executed again. That is, the collection of plasma is continued.

[Blood Component Returning Step] As shown in FIG. 4, in the blood component returning step, first, the weight sensor 16 detects the current collection amount Y (step S20).
1) It is determined whether or not the current collection amount Y has reached the target collection amount X (step S202).

If the current collection amount Y has not reached the target collection amount X, then the target return amount W is calculated (step S203). The target return amount W is, as described above,
It is represented by the above equation (1).

Next, the first liquid feed pump 11 is rotated in reverse (4
(5 ml / min) (step S204). As a result, the remaining blood components in the centrifuge 20 are returned to the donor.

Next, it is determined whether or not the return amount V measured by the control unit 55 has reached the target return amount W (step S205), and the return amount V has reached the target return amount W. If not, it is determined whether or not the first bubble sensor 17 has detected air (step S206).

If the first bubble sensor 17 has not detected air, the flow returns to step S205 again, and returns to step S2.
05 and later are performed. That is, the return of the remaining blood components is performed until the return amount V reaches the target return amount W, or until the first bubble sensor 17 detects air.

If the first bubble sensor 17 has detected air, the rotation speed of the first liquid supply pump 11 is reduced by one step (for example, 30 ml / min), and the rotation is performed a predetermined number of times (for example, 10 ml / min).
Times) (step S207). As a result, the remaining blood components in the chamber 21d are returned from the first bubble sensor 17 to the blood donor.

Then, the rotation of the first liquid supply pump 11 is stopped (step S208), the blood component return step is completed, and the flow proceeds to the next blood component collection step.

If the return amount V has reached the target return amount W in step S205, the rotation of the first liquid supply pump 11 is stopped (step S208), and the blood component return step is terminated. Then, the process proceeds to the next blood component collecting step.

In step S202, if the current collection amount Y has reached the target collection amount X, the first liquid supply pump 11 is rotated (step S209), and the first bubble sensor 17 Is detected (step S210),
The rotation speed of the first liquid sending pump 11 is reduced by one step (for example, 30 ml / min) and rotated a predetermined number of times (for example, 10 times) (step S211). Then, the rotation of the first liquid supply pump 11 is stopped (step S212), and the blood component return step is completed. The collection of the plasma is terminated when the blood component return step is completed.

As described above, according to the blood component collecting apparatus 1, in the blood component return step, the target return amount W is calculated each time, and the information is used to return the remaining blood components to the donor. If the amount of plasma to be collected in the next blood component collection step is small,
With the remaining blood components remaining within 0, the process proceeds to the blood component collection step. Thereby, in the blood component return step, the amount of return is reduced and the time required for the return is reduced, and in the next blood component collection step, the amount of blood collected is reduced, and the time required for the blood collection is reduced. Be shortened. That is, the total blood collection amount (whole blood processing amount) in collecting blood components is reduced, and the required time is shortened.

As a result, the time during which the donor is restrained and the occupancy time of the bet can be reduced, and the burden on the donor can be reduced.

Since the target return amount W is calculated by the above equation (1), the target return amount W can be obtained properly and reliably.

The blood component collecting apparatus according to the present invention has been described based on the illustrated embodiments. However, the present invention is not limited to these, and the configuration of each part may be any configuration having the same function. Can be replaced with

Further, in this embodiment, the case where the blood component collecting apparatus is applied to the plasma collecting apparatus has been described. Can also be adapted.

[0119]

Next, specific examples of the present invention will be described.

<Example> Plasma was collected from a donor using the blood component collecting apparatus 1 of the embodiment shown in FIG. 1, and the amount of blood processing and the time required for collecting the plasma were measured.

In this measurement, the blood component collecting step
One cycle was composed of two steps, a blood component return step after the blood component collection step. The collection of plasma was completed in two cycles.

The amount of blood introduced from the donor into the blood component collection circuit 2 was defined as the blood processing amount.

The amount of collected plasma was defined as the amount of collected plasma, and the time required for collecting the plasma was defined as the collection time.

The amount of the remaining blood component returned to the donor was defined as the return amount, and the time required for returning the remaining blood component was defined as the return time.

The time required for the entire cycle (collection time and return time) was taken as the required time.

In this measurement, the hematocrit value of the donor was 45%, and the target collection amount X was 300 m
l.

The target return amount W was obtained from the above equation (1), and the coefficient Z of the equation (1) was set to 2.

The blood collection speed was 60 ml / mi.
n, the return rate of the remaining blood components was 45 ml / min. The capacity of the centrifugal bowl 20 was 265 ml.

The addition amount of the ADC-A solution was set at 1/12 of the blood processing amount at the time of plasma collection.

The blood processing volume and the required time required for the collection of the plasma (required for the whole cycle) are as shown in Table 1 below.

Table 1 also shows the blood processing volume, plasma collection volume, collection time, target return volume, return volume, and return time in each cycle.

<Comparative Example> In the blood component return step, plasma was collected from a donor using a blood component collection device configured to return all remaining blood components remaining in the blood component collection circuit. Blood processing volume and time required for plasma collection were measured.

In this measurement, the conditions were the same as in the above example, except that all the remaining blood components were returned to the donor.

The blood processing amount and the time required for the collection of the plasma (required for the whole cycle) are as shown in Table 1 below.

Table 1 also shows the blood processing amount, plasma collection amount, collection time, return amount and return time in each cycle.

[0136]

[Table 1]

<Evaluation> In the example, as shown in Table 1,
In cycle 1, the value of the target return amount W becomes less than the capacity of the centrifugal bowl 20 (target return amount = 132 ml),
A part of the remaining blood components in the centrifuge bowl 20 was returned to the donor (return amount = 132 ml). Therefore, the return time is short (return time = 2.9 min).

Since the remaining blood components remained in the centrifugal bowl 20, in cycle 2, the blood processing volume was small (blood processing volume = 215 ml) and the collection time was short (collection time = 3.9 min).

On the other hand, in the comparative example in which all the remaining blood components were returned in each blood component return step, as shown in Table 1, all the remaining blood components in the centrifugal bowl 20 were returned in cycle 1 ( Return amount = 249 ml), return time is longer than that of the embodiment (return time = 5.5 mi)
n).

In the cycle 2, since the inside of the centrifugal bowl 20 is empty, the blood processing volume is larger than that of the embodiment (blood processing volume = 292 ml), and the collection time is longer than that of the embodiment (collection time = 5.5 min).

As shown in Table 1, the amount of blood processed in all the cycles in the example was smaller than that in the comparative example (634 ml in the example, 711 ml in the comparative example).
Also, the required time is short (in the case of the embodiment = 19.9 mi)
n, for the comparative example = 23.9 min).

[0142]

As described above, according to this blood component collecting apparatus, it is possible to efficiently collect blood components.

Particularly, in the blood component return step, a target return amount is calculated, and the remaining blood components are returned to the donor using the information. Thereby, the return amount is reduced and the return time is shortened.

When the return amount is reduced, the blood collection amount (blood processing amount) is reduced in the next blood component collection step, and the blood collection time is shortened.

As a result, the total amount of blood to be collected in collecting blood components is reduced, and the required time is shortened.

As a result, it is possible to shorten the time for which the donor is restrained and the time for occupying the bet, and it is possible to reduce the burden on the donor.

[Brief description of the drawings]

FIG. 1 is a plan view showing a configuration example of a blood component collection device of the present invention.

FIG. 2 is a diagram showing a cross section of a centrifuge driving device used in the blood component collecting device of the present invention.

FIG. 3 is a flowchart showing a control operation of a control unit in a blood component collecting step.

FIG. 4 is a flowchart showing a control operation of a control unit in a blood component return step.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 blood component collecting device 2 blood component collecting circuit 10 centrifuge driving device 11 first liquid feed pump 12 second liquid feed pump 14 turbidity sensor 15 optical sensor 16 detecting means 17 first bubble sensor 18 second Bubble sensor 20 Centrifuge 21 First line 22 Second line 23 Third line 24 Fourth line 25 Blood component collection bag 29 Blood collection needle 32 Subbag 33 Cassette housing 51 First channel opening / closing means 52 Second flow path opening / closing means 55 Control unit 131 Plasma layer 132 Buffy coat layer 133 Red blood cell layer 142 Rotor 143 Inflow port 144 Outflow port 145 Upper part 151 Centrifuge drive housing 152 Leg 153 Motor 154 Rotating shaft 155 Fixed table 156 Bolt 157 Spacer 158 Mounting member 21a Blood sampling needle side first In 21b centrifuge first line 21c connecting branch connector 21d chamber 21g first pump tube 21i filter 23a second pump tube 23c bubble removing chamber 23d anticoagulants 溶器 connection needle 32a tube

Claims (8)

[Claims] 1. A blood collection means for collecting blood from a donor, and a rotor having a blood storage space therein, and a centrifuge for centrifuging the blood collected by the blood collection means in the blood storage space by rotation of the rotor. And a blood component collection circuit comprising a blood component collection bag for collecting a predetermined blood component separated by the centrifuge, and centrifuging blood collected from a donor to remove the predetermined blood component. A blood component collection device for returning the remaining blood components to the blood donor after the blood collection, wherein the detection means detects the amount of the predetermined blood component collected from the blood donor, and the blood component is collected from the blood donor. Based on the target collection amount of the predetermined blood component and the current collection amount of the predetermined blood component detected by the detection means, the remaining blood component to be returned to the donor is Calculating means for calculating the return amount of the target, using the information obtained by the calculating means, the blood component is configured to return the remaining blood component to the blood donor Sampling equipment. 2. A blood collection means for collecting blood from a donor, and a rotor having a blood storage space therein, and a centrifuge for centrifuging the blood collected by the blood collection means in the blood storage space by rotation of the rotor. And a blood component collection circuit comprising a blood component collection bag for collecting a predetermined blood component separated by the centrifuge, and centrifuging blood collected from a donor to remove the predetermined blood component. A blood component collection device that alternately and repeatedly performs a blood component collection process of collecting and a blood component return process of returning the remaining blood components to the blood donor, wherein the predetermined blood component collected from the blood donor is collected. Detection means for detecting the amount; a target collection amount of the predetermined blood component collected from the blood donor; and a current collection amount of the predetermined blood component detected by the detection means. Calculating means for calculating a target return amount of the remaining blood component to be returned to the blood donor based on the information obtained by the calculating means. A blood component collection device configured to return a component. 3. The method according to claim 1, wherein the current sampling amount is a sampling of the predetermined blood component detected by the detecting unit after the predetermined blood component is collected and before the remaining blood component is returned to the blood donor. The blood component collection device according to claim 1 or 2, wherein the device is a quantity. 4. The blood according to claim 1, wherein the return amount of the target is a value obtained by multiplying a difference between the target collection amount and the current collection amount by a predetermined coefficient. Component sampling device. 5. When returning the remaining blood component to the blood donor, when the return of the remaining blood component to the blood donor is completed without reaching the target return amount,
The blood component collection device according to any one of claims 1 to 4, wherein return of the remaining blood components to the donor is completed. 6. When returning the remaining blood component to the donor, when the return amount of the remaining blood component to the donor reaches the target return amount, the remaining blood component is returned. 2. The return of the component to the donor is terminated.
6. The blood component collecting apparatus according to any one of claims 5 to 5. 7. The blood component collection device according to claim 1, wherein when the current collection amount reaches the target collection amount, all of the remaining blood components are returned to the blood donor. . 8. The blood component collecting apparatus according to claim 1, wherein the predetermined blood component is mainly plasma.