KR102404002B1 - Driving method of centrifuge device - Google Patents

Abstract

이다.The present embodiments relate to a method of driving a centrifugal separation device. A method of driving a centrifugal separation device according to an embodiment of the present invention is centrifugal separation including a main body including a separation unit in which each component of a centrifuged sample is separated and located and an extraction unit to move and receive each component located in the separation unit In the driving method of a centrifugal separation device including a chamber, wherein the separation unit is positioned side by side in one direction and includes a first separation unit and a second separation unit separated by a first partition wall, wherein the centrifugal separation chamber is at an optimum rotation speed or less When the first inclination angle of the first partition wall is θ1, the average rotation radius of the centrifugation chamber is r, the gravitational acceleration is G, and the optimum rotation speed is n, the optimum expressed in revolutions per second rotation speed is

to be.

Description

A method of driving a centrifugal separator {DRIVING METHOD OF CENTRIFUGE DEVICE}

The present embodiment relates to a method of driving a centrifugal separation device, and more particularly, to a method of driving a centrifugal separation device capable of easily extracting components located in an upper layer among samples separated by components without contamination.

Whole blood or various biological fluids may be separated from its components or parts. For example, whole blood is composed of plasma, white blood cells, platelets, and red blood cells, and may be separated for each component in a centrifugal separation device or the like.

In the prior art, for example, in order to separate whole blood for each component, whole blood was put into a single tube container and then put into a centrifugal separator to separate based on specific gravity. The centrifuged sample was stacked in several layers for each component based on specific gravity, and each component layered and stacked in this way was manually recovered.

However, in general, when each layer of a sample stacked for each component is separated by hand, work efficiency is remarkably reduced, and each centrifuged component is mixed again, and there is no choice but to extract a specific component. However, there was a limit to complete extraction. In addition, when various samples are simultaneously processed in a narrow space, there is a problem that cross-contamination often occurs.

Therefore, when a biological fluid such as whole blood is separated using a centrifugal separation device, it is urgent to develop a technology capable of easily extracting a desired component without re-contamination in the extraction process after separating the fluid for each component.

In one embodiment, an object of the present invention is to provide a method of driving a centrifugal separation device capable of maximally securing a supernatant after centrifugation of a sample.

The method of driving the centrifugal separation device according to the present embodiment is centrifugal separation including a main body including a separation unit in which each component of a centrifuged sample is separated and located, and an extraction unit to move and receive each component located in the separation unit In the driving method of a centrifugal separation apparatus including a chamber, wherein the separation unit is positioned side by side in one direction and includes a first separation unit and a second separation unit separated by a first partition wall, the centrifugal separation chamber is located on the axis of rotation It rotates below the optimum rotation speed, and when the first inclination angle of the first partition wall is θ1, the average rotation radius of the centrifugal separation chamber is r, the gravitational acceleration is G, and the optimum rotation speed is n, the number of revolutions per second The optimal rotation speed expressed is

to be.

When the centrifugal separation chamber is rotated, a second inclination angle of the water surface of the sample positioned in the first separation unit may be smaller than the first inclination angle of the first partition wall.

The average rotation radius of the centrifugation chamber may be a distance from the center of the rotation axis to the water surface.

The maximum value of the average rotation radius of the centrifugal separation chamber may be a distance from the center of the rotation shaft to the outlet located on the bottom surface of the first separation unit.

The separation unit further includes a third separation unit separated by a second partition wall, the sample is centrifuged, the first separation liquid is located in the first separation unit, and the second separation liquid is located in the second separation unit; A third separation liquid may be located in the third separation unit.

In the initial stage of driving the centrifugation chamber, the centrifugation chamber is rotated faster than the optimal rotation speed to move the first separation solution to the extraction unit, and in the final stage of driving the centrifugation chamber, the centrifugation chamber is The first separation liquid may be moved to the extraction unit by rotating it below the optimum rotation speed.

The reference time point for dividing the initial stage and the final stage may be a time point at which the first separation liquid stops moving to the extraction unit.

In the initial stage, the first separation liquid may be moved to the extraction unit through an outlet located on a bottom surface of the first separation unit adjacent to the first partition wall.

The sample may include blood, and the first separation liquid positioned in the first separation unit may include plasma.

The centrifugation chamber may include: an upper plate including an inlet for injecting a sample for centrifugation and an outlet for withdrawing the separated components accommodated in the extraction unit to the outside; And it may further include a lower plate for sealing the opening of the lower body.

In the method of driving the centrifugal separation device according to an embodiment, the centrifugal separation chamber is rotated at an optimum rotation speed or less so that the second inclination angle of the water surface of the sample is smaller than or equal to the first inclination angle of the first partition wall, thereby separating the first separation unit. The supernatant of the sample to be used can be secured as much as possible.

1 is a schematic perspective view of a centrifugal separation device according to an embodiment;
2 is a plan view schematically illustrating a sub-centrifugal separation chamber of a centrifugal separation apparatus according to an embodiment.
3 is an exploded perspective view of the sub-centrifuge chamber of FIG. 2 .
4 is a cross-sectional view illustrating the sub-centrifuge chamber of FIG. 2 taken along line IV-IV'.
5 to 7 are enlarged views of part A of FIG. 4 , and FIG. 5 is a view showing the state of the water surface of the first separation liquid when the centrifugal separation chamber does not rotate.
6 is a view showing the state of the water surface of the first separation liquid when the centrifugal separation chamber rotates at a speed greater than the optimum rotation speed.
7 is a view showing the state of the water surface of the first separation liquid when the centrifugal separation chamber rotates at a speed less than or equal to the optimum rotation speed.

Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and the same reference numerals are given to the same or similar components throughout the specification.

In addition, since the size and thickness of each component shown in the drawings are arbitrarily indicated for convenience of description, the present invention is not necessarily limited to the illustrated bar. In order to clearly express various layers and regions in the drawings, the thicknesses are enlarged. And in the drawings, for convenience of description, the thickness of some layers and regions are exaggerated.

Further, when a part of a layer, film, region, plate, etc. is said to be “on” or “on” another part, it includes not only cases where it is “directly on” another part, but also cases where another part is in between. . Conversely, when we say that a part is "just above" another part, we mean that there is no other part in the middle. In addition, to be "on" or "on" the reference part is located above or below the reference part, and does not necessarily mean to be located "on" or "on" the opposite direction of gravity. .

In addition, throughout the specification, when a part "includes" a certain component, this means that other components may be further included, rather than excluding other components, unless otherwise stated.

In addition, throughout the specification, when referring to "planar", it means when the target part is viewed from above, and "cross-sectional" means when viewed from the side when a cross-section of the target part is vertically cut.

Hereinafter, a method of driving a centrifugal separation device according to an embodiment will be described in detail with reference to the drawings.

1 is a schematic perspective view of a centrifugal separation device according to an embodiment;

As shown in FIG. 1 , the centrifugal separation apparatus according to an embodiment includes a centrifugal separation chamber 1000 and a rotational driving unit 2000 configured to rotate the centrifugal separation chamber 1000 .

The centrifugal separation chamber 1000 includes a plurality of sub centrifugal separation chambers 1100 , and the sub centrifugal separation chamber 1100 may have a planar sectoral shape. Accordingly, the centrifugal separation chamber 1000 including the plurality of sub centrifugal separation chambers 1100 may have a planar circular shape as a whole. In this case, the plurality of sub centrifugal separation chambers 1100 may be sequentially arranged adjacent to each other.

When the centrifugal separation chamber 1000 is manufactured as a single structure by injection or the like, it is difficult to completely implement the complex internal structure of the centrifugal separation chamber 1000 . Therefore, in the present embodiment, by dividing the centrifugal separation chamber 1000 into a plurality of sub-centrifugal separation chambers 1100 and manufacturing, the quality and high productivity of the injection product can be maintained.

In the present embodiment, the centrifugal separation chamber is composed of four sub-centrifugation chambers, but is not limited thereto, and may include a variety of sub-centrifugation chambers.

The rotation driving unit 2000 may rotate the centrifugal separation chamber 1000 at a predetermined rotation speed based on the rotation axis Y to separate the sample 1 injected into the centrifugal separation chamber 1000 into components.

Hereinafter, a sub-centrifugal separation chamber of the centrifugal separation apparatus according to an exemplary embodiment will be described in detail with reference to FIGS. 2 to 4 .

2 is a plan view schematically showing a sub-centrifuge chamber of the centrifugal separation apparatus according to an embodiment, FIG. 3 is an exploded perspective view of the sub-centrifuge chamber of FIG. 2, and FIG. 4 is the sub-centrifuge chamber of FIG. It is a cross-sectional view cut along line IV-IV'.

2 to 4 , the sub centrifugal separation chamber 1100 includes an upper plate 100 , a body 200 , and a lower plate 300 .

The main body 200 includes a separation unit 210 in which the centrifuged sample 1 is separated for each component, and an extraction unit 220 to move and receive the sample 1 separated by component in the separation unit 210 . includes

The separation unit 210 includes a first separation unit 211 , a second separation unit 212 , and a third separation unit 213 positioned side by side in one direction. The separation unit 210 is disposed outside the first separation unit 211 , the second separation unit 212 , and the third separation unit 213 in the order of the rotation axis Y (refer to FIG. 1 ). At this time, each region of the first separation unit 211 and the second separation unit 212 is separated by a first partition wall 311 located in the separation unit 210 , and the second separation unit 212 and the second separation unit 212 are separated from each other. Each region of the third separation unit 213 is separated by a second partition wall 312 .

The first partition wall 311 may be formed to have a predetermined distance H1 from the upper plate 100 . The second partition wall 312 may be formed to have a predetermined distance H2 from the upper plate 100 . In this embodiment, the gap H1 between the first partition wall 311 and the upper plate 100 and the gap H2 between the second partition wall 312 and the upper plate 100 are not necessarily the same. This can be appropriately adjusted according to the characteristics of the component to be separated, for example, whether it is a solid component or a liquid component, and the amount of the component to be separated.

In addition, the first partition wall 311 and the second partition wall 312 may have a structure that protrudes from the lower plate 300 , for example, and protrudes from a sidewall inside the separation part 210 of the main body 200 . It may have a structure in the form in which it is applied, and the form is not particularly limited.

After centrifugation, the sample (1) is separated into the first separation solution (2) in the first separation unit (211), and is separated into the second separation solution (3) in the second separation unit (212), The third separation liquid 4 may be separated and located in the separation unit 213 .

When the sample 1 is blood, the first separation solution 2 is plasma, the second separation solution 3 is a buffy coat containing a large number of white blood cells, and the third separation solution 4 ) may be a component containing a plurality of red blood cells.

Therefore, when the second separator 212 and the first separator 211 are filled by injecting blood into the third separator 213 for centrifugation, and then centrifugation is performed, it is located in the third separator 213 Among the components of the blood, the component corresponding to the buffy coat moves to the area of the second separation unit 212 on the second partition wall 312 , and includes a large number of red blood cells among components of the blood located in the second separation unit 212 . The component to move to the third separation unit 213 riding on the second partition wall 312 .

As described above, since the separation unit 210 includes the first separation unit 211 , the second separation unit 212 , and the third separation unit 213 , each component of the centrifuged sample 1 is separated by component. The first separation unit 211 , the second separation unit 212 , and the third separation unit 213 may be respectively located.

The main body 200 includes an injection hole extension 271 , and the injection hole extension portion 271 is connected to the injection path 341 located under the main body 200 .

When a sample is injected into the inlet 101 of the upper plate 100 for centrifugal separation of the desired sample 1, the sample 1 injected through the inlet extension 271 and the injection path 341 is separated by a third Go to section 213 .

At this time, when the first vent hole 131 is opened, the sample injected into the third separation unit 213 by air circulation moves to the second separation unit 212 along the second partition wall 312 , and thereafter, the first The partition wall 311 may be moved toward the first separation unit 211 . In the present embodiment, the separation unit 210 is divided into three regions of the first separation unit to the third separation unit 211 , 212 , and 213 , but the present invention is not limited thereto. or may join.

The first vent hole 131 is preferably located in a region close to the rotation axis Y of the centrifugal separator in the upper plate 100 . In addition, it is preferable to fill the sample 1 to be injected for centrifugation, for example, blood from the third separation unit 213 located farthest from the center of the rotation axis Y of the centrifugal separation device. do. In this case, since the air pushed by the injected sample 1 escapes through the upper first vent hole 131, sample injection is smooth. In addition, it is possible to quickly and easily inject the sample into the sub-centrifuge chamber 1100 without damaging the active ingredient, for example, cells, etc. contained in the sample 1 because no bubbles are formed in the sample 1 have.

Meanwhile, the main body 200 includes an extraction unit 220 , and the extraction unit 220 includes a first extraction unit 221 , a second extraction unit 222 , and a third extraction unit 223 .

The first extraction unit 221 is a space for moving and accommodating the components separated by the first separation unit 211 , and the second extraction unit 222 is a space for additionally separating the components moved to the first extraction unit 221 . It is a space for moving and accommodating additional separated components afterward.

Specifically, the first separation unit 211 is connected to the first extraction path 321 located in the lower portion of the main body (200). The components separated by the first separation unit 211 after centrifugation are moved through the first extraction path 321 connected to the first separation unit 211 and accommodated in the first extraction unit 221 .

The body 200 includes a first valve unit 231 positioned to overlap a partial region of the first extraction path 321 . At this time, the first valve is positioned in the first valve unit 231 , and the first extraction path 321 is opened and closed by the first valve. Therefore, in order to extract the components separated by the first separation unit 211 after centrifugation, the components separated in the first separation unit 211 are removed by opening the first extraction path 321 by driving the first valve. 1 may be moved to the extraction unit 221 .

After being separated by the first separation unit 211 as described above, the components accommodated in the first extraction unit 221 through the first extraction path 321 are further separated by performing centrifugation once more for the first extraction. It may be moved to the second extraction unit 222 through the second extraction path 322 connected to the unit 221 . In this case, it is possible to more reliably remove the contaminants from the components separated by the centrifugal separation unit 211 in the first separation unit 211 .

When, for example, blood is injected into the sample, the first separation solution 2 , which is the supernatant of the sample 1 positioned in the first separator 211 through centrifugation, may be plasma.

Such plasma is used for genomic analysis of cell free DNA, which is used as a method of liquid biopsy to perform a diagnosis using a part of a specific tissue secreted into blood without directly extracting the patient's tissue. That is, in the genomic analysis of free DNA, a method of increasing the accuracy of diagnosis by centrifuging the plasma obtained after separating the components of whole blood twice to remove contaminants is common. In this case, it is necessary to manually divide the components of the blood by changing several tubes, and to centrifuge them in succession again. cause cost increase.

However, as in the present embodiment, after plasma is primarily separated in the first extraction unit 221 and additional centrifugation is performed, the supernatant separated in the first extraction unit 221 is transferred to the second extraction unit 222 . By accommodating it, it is possible to obtain a plasma that is not contaminated by other DNA components.

However, after component separation using the centrifugation chamber 1000 is completed, the centrifugation chamber 1000 is moved to move the first separation solution 2, which is the supernatant of the sample 1, to the first extraction unit 221 . When rotating, when the second inclination angle θ2 of the water surface 1a of the sample 1 positioned in the first separation unit 211 is greater than the first inclination angle θ1 of the first partition wall 311, the first Since the extraction path 321 and the first separation liquid 2 do not meet each other, the first separation liquid 2 does not all move to the first extraction unit 221 and rotates while remaining in the first separation unit 211 . Therefore, it is impossible to secure all of the first separation liquid 2 remaining in the first separation unit 211 .

Accordingly, in the present embodiment, the second inclination angle θ2 of the water surface 1a of the sample 1 positioned in the first separation unit 211 is maintained to be less than or equal to the inclination angle θ1 of the first partition wall 311 . and rotating the centrifugal separation chamber 1000 below the optimum rotation speed to maintain the first extraction path 321 and the water surface 1a to meet until the first separation liquid 2 is not left. The separation liquid 2 can be moved to the first extraction unit 221 .

This will be described in detail below with reference to FIGS. 5 to 7 .

5 to 7 are enlarged views of part A of FIG. 4 , FIG. 5 is a view showing the state of the water surface of the first separation liquid when the centrifugal separation chamber does not rotate, and FIG. 6 is an optimal centrifugal separation chamber It is a view showing the state of the water surface of the first separation liquid when rotating at a speed greater than the rotation speed, and FIG. 7 is the water surface of the first separation liquid when the centrifugal separation chamber rotates at a speed less than or equal to the optimum rotation speed It is a diagram showing the state of

As shown in FIG. 5 , when the centrifugal separation chamber 1000 does not rotate, the water surface 1a of the first separation liquid 2 located in the first separation unit 211 is the surface of the centrifugal separation chamber 1000 . It may be formed parallel to the bottom surface (20a). That is, the water surface 1a may be formed perpendicular to the direction of gravity Fg. In this case, the first separation liquid 2 may be positioned to be separated from the second separation liquid 3 by the first partition wall 311 having a first inclination angle θ1 .

6 and 7, when the centrifugal separation chamber 1000 rotates at a predetermined rotation speed, the water surface 1a of the first separation liquid 2 is moved by the centrifugal force Fc by the centrifugal separation chamber ( 1000) has a bottom surface 20a and a second inclination angle θ2.

At this time, as shown in FIG. 6 , when the centrifugal separation chamber 1000 rotates faster than the optimum rotation speed n, the water surface ( The second inclination angle θ2 of 1a) is greater than the first inclination angle θ1 of the first partition wall 311 . Centrifugal separation chamber 1000 As such, the second inclination angle θ2 of the water surface 1a of the first separation liquid 2 positioned in the first separation unit 211 is the first inclination angle θ2 of the first partition wall 311 ( When θ1 is greater than θ1 , the first separation liquid 2 is not all moved to the first extraction unit 221 , but remains in the first separation unit 211 , making it difficult to secure the first separation liquid 2 as much as possible.

However, as shown in FIG. 7 , when the centrifugal separation chamber 1000 rotates below the optimum rotation speed n, the water surface 1a of the first separation liquid 2 positioned in the first separation unit 211 is ) is separated through the first extraction path 321 while maintaining the second inclination angle θ2 less than or equal to the first inclination angle θ1 of the first partition wall 311 . Accordingly, the first separation liquid 2 , which is the supernatant of the sample 1 located in the direction close to the central axis Y among the upper portions of the first partition wall 311 , can be secured as much as possible.

The optimum rotation speed n expressed in such rotations per second (RPS) may be expressed by Equation 1 below.

where r is the average turning radius of the centrifugation chamber, and G is the gravitational acceleration. 7 , the average rotation radius r of the centrifugal separation chamber 1000 may be the distance from the center of the rotation axis Y to the water surface 1a. In particular, the maximum value of the average rotation radius r is the lowest due to the rotation of the centrifugal separation chamber 1000 so that the water surface 1a is parallel to one side of the first partition wall 311 and the water surface 1a ) may be the distance from the center of the rotation axis Y to the water surface 1a when the second inclination angle θ2 is equal to the first inclination angle θ1. Therefore, the maximum value of the average rotation radius r is the air outlet ( 50) can be a distance. Here, the bottom part 31 of the first partition wall 311 is a boundary line between the first partition wall 311 and the bottom surface 20a, and the outlet 50 is adjacent to the first partition wall 311 side and the bottom surface 20a. can be located in

The resultant force Ft applied to the water surface 1a of the first separation solution 2 is the sum of gravity Fg and centrifugal force Fc, and tan(θ1) = Fc/Fg, so that Equation 1 can be derived. At this time, the closer the outlet 50 is to the bottom part 31 that is the boundary line between the first partition wall 311 and the bottom surface 20a, the more advantageous it is to move the first separation liquid 2 to the first extraction part 221 . do. Accordingly, it is possible to secure the first separation liquid 2 as much as possible.

Table 1 is a table showing the optimal number of rotations according to the first inclination angle when the average rotation radius r is set to 3 cm.

θ1 tan(θ1) RPM (revolutions per minute, speed) RPS (revolutions per second, speed) 45 One 172.6 2.877 60 1.73 227.1 3.786 70 2.75 286.1 4.768 75 3.73 333.4 5.557 80 5.67 411 6.85 85 11.43 583.5 9.725 87 19.08 753.9 12.565 89 57.29 1306.4 21.773

As shown in Table 1, it can be seen that the optimal rotation speed n increases as the first inclination angle θ1 increases.

In addition, by rotating the centrifugal separation chamber 1000 at the optimum rotation speed n, the first separation unit 211 can effectively use the space for containing the first separation liquid 2, thereby reducing the dead space. can be minimized

On the other hand, in the initial stage of driving the centrifugal separation chamber 1000 in order to secure the first separation liquid 2 as much as possible, the centrifugal separation chamber 1000 is rotated faster than the optimum rotation speed n, and the centrifugal separation chamber 1000 is rotated faster than the optimum rotation speed n. In the final stage of driving of the centrifugal separation chamber 1000 may be rotated at the optimum rotation speed (n). Here, the reference point for dividing the initial stage and the final stage is that the first extraction path 321 and the first separation liquid 2 do not meet each other, so that the first separation liquid 2 moves to the first extraction unit 221 . It may be a stopping point.

That is, in the initial stage of driving the centrifugal separation chamber 1000 , by rotating the centrifugal separation chamber 1000 faster than the optimum rotation speed n, the first separation liquid 2 located in the first separation unit 211 is strongly strengthened. While maintaining the separated state using centrifugal force, it is quickly moved to the first extraction unit 221 through the first valve.

In this way, since the centrifugal separation chamber 1000 can rapidly move the first separation liquid 2 to the first extraction unit 221 as it rotates at a higher speed, the centrifugal separation chamber 1000 is optimized for separation efficiency. It may be advantageous to rotate faster than the rotation speed n.

However, as shown in FIG. 6 , at this speed, due to the strong centrifugal force, the first separation liquid 2 remaining in the centrifugal separation chamber 1000 is closely attached to the first partition wall 311 and the outlet located on the bottom surface 20a. (50) cannot escape.

Accordingly, at the reference point in time, the second inclination angle θ2 of the water surface 1a is greater than the first inclination angle θ1, so that the first separation liquid 2 stops moving to the first extraction unit 221, and the first extraction unit ( The first separation liquid 2 that has not been discharged to 221 ) remains in the first separation unit 211 .

However, as shown in FIG. 7 , by slowly rotating the centrifugation chamber 1000 below the optimum rotation speed n in the final stage of driving the centrifugation chamber 1000 after the reference point, the first separation solution ( 2) by moving the water surface 1a beyond the location of the outlet 50 to move the first separation liquid 2 remaining in the first separation unit 211 to the first extraction unit 221 through the outlet. .

Through this series of processes, the first separation liquid 2 can be sent to the first extraction unit 221 as much as possible, so that the first separation liquid 2 can be secured as much as possible.

On the other hand, the first extraction part 221 includes a second vent hole extension part 252 on the sealed upper surface, and the second vent hole extension part 252 is a second vent hole located in the upper plate 100 . It is a structure that forms one body with (132). The second vent hole 132 is opened when the component separated by the first separation unit 211 is moved to the first extraction unit 221 , that is, when the first valve is opened, the second vent hole 132 is also opened. it should be In addition, when the component additionally separated by the first extraction unit 221 is moved to the second extraction unit 222 , that is, when the second valve is opened, the second vent hole 132 must also be opened. When the first valve is closed, the second vent hole 132 may also be closed.

The first extraction unit 221 is connected to the second extraction path 322 located at the lower portion of the main body 200 . The components additionally centrifuged as described above in the first extraction unit 221 are moved through the second extraction path 322 connected to the first extraction unit 221 and accommodated in the second extraction unit 222 .

The second extraction unit 222 includes a first extraction unit 241 . The first draw-out part 241 may have a conical structure in which the upper and lower surfaces of the main body 200 are open and the upper part has a larger diameter than the lower part. In addition, the upper opening of the first draw-out part 241 has a structure integral with the first draw-out hole 121 positioned on the upper plate 100 .

In this way, the component further separated and accommodated in the second extraction unit 222 may be withdrawn to the outside through the first outlet 121 . In this case, the withdrawal to the outside through the first outlet 121 may be performed using, for example, a pipette.

Next, the second separation unit 212 is connected to the third extraction path 323 located in the lower portion of the main body (200). The components separated by the second separator 212 after centrifugation are moved through the third extraction path 323 connected to the second separator 212 and accommodated in the third extraction part 223 .

The body 200 includes a third valve unit 233 positioned to overlap a partial region of the third extraction path 323 . At this time, the third valve is positioned in the third valve unit 233 , and the third extraction path 323 is opened and closed by the third valve. Therefore, in order to extract the components separated by the second separator 212 after centrifugation, the third valve is driven to open the third extraction passage 323 to remove the separated components in the second separator 212 . 3 It can be moved to the extraction unit 223 .

The third extractor 223 includes a second extractor 242 . The second lead-out part 242 may have a conical structure in which the upper and lower surfaces of the main body 200 are open and the upper part has a larger diameter than the lower part. In addition, the upper opening of the second lead-out part 242 has a structure integral with the second lead-out hole 122 positioned on the upper plate 100 .

As described above, the component accommodated in the third extractor 223 may be withdrawn to the outside through the second outlet 122 . In this case, the withdrawal to the outside through the second outlet 122 may be performed using, for example, a pipette.

The upper plate 100 is to prevent contamination when injecting a sample for centrifugation or withdrawing each centrifuged component to the outside, and includes an inlet 101 and outlet ports 121 and 122 .

The inlet 101 is for injecting a sample into the separation unit before centrifugation, and the outlet 101 is for extracting each component of the centrifuged sample for each component.

The outlet includes a first outlet 121 and a second outlet 122 .

In this embodiment, the first outlet 121 is for withdrawing the components accommodated in the second extraction unit 222 among the components of the centrifuged sample to the outside. In this embodiment, the component accommodated in the second extraction unit 222 is relatively dense after moving the component separated in the first separation unit 211 to the first extraction unit 221 after centrifugation, for example. It is a liquid component with high purity by further separating the low component and moving it to the second extraction unit 222 .

In addition, the second outlet 122 is for taking out the components accommodated in the third extraction unit 223 among the components of the centrifuged sample to the outside. The upper plate 100 includes a first vent hole 131 and a second vent hole 132 together with an inlet and an outlet.

The first vent hole 131 is located on the upper surface of the first separation unit 211 located in the main body 200 . If necessary, when the upper surface of the separation unit 210 has a closed structure, a first vent hole extension (not shown) may be formed on the upper surface of the first separation unit 211 .

The second vent hole 132 is connected to the second vent hole extension part 252 included in the first extraction part 221 located in the main body 200 .

In addition, the upper plate 100 includes a first valve hole 111 , a second valve hole 112 , and a third valve hole 113 .

The first valve hole 111 is connected to the first valve unit 231 . The first valve unit 231 is positioned to overlap a partial region of the first extraction path 321 positioned in the main body 200 . The first valve located in the first valve unit 231 is driven through the first valve hole 111, and the first separation unit 211 by opening and closing the first extraction path 321 through the first valve It is possible to control the movement of separated components into the

The second valve hole 112 is connected to the second valve unit 232 . The second valve unit 232 is positioned to overlap a partial region of the second extraction path 322 positioned in the main body 200 . The second valve located in the second valve unit 232 is driven through the second valve hole, and by opening and closing the second extraction path 322 through the second valve, You can control movement.

The third valve hole 113 is connected to the third valve unit 233 . The third valve unit 233 is positioned to overlap a partial region of the third extraction path 323 . The third valve located in the third valve unit 233 is driven through the third valve hole 113 and is connected to the second separation unit 212 by opening and closing the third extraction path 323 through the third valve. The movement of separated components can be controlled.

The lower plate 300 seals the opening partially formed in the lower portion of the main body 200 .

In this embodiment, the structure in which the first partition wall 311 and the second partition wall 312 are formed in the main body 200 is illustrated. However, the first partition wall 311 and the second partition wall 312 may be formed to protrude from the lower plate 300 if necessary.

The present invention is not limited to the above embodiments, but can be manufactured in various different forms, and those of ordinary skill in the art to which the present invention pertains can take other specific forms without changing the technical spirit or essential features of the present invention. It will be understood that it can be implemented as Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

One; sample
2; first separation liquid
3; second separation liquid
100: centrifugation chamber
110: sub centrifugation chamber
200: rotation drive unit
211: first separation unit
212: second separation unit
213: third separation unit
311: first bulkhead

Claims (10)

In a method of driving a centrifugal separation device comprising a centrifugal separation chamber comprising a main body including a separation unit in which each component of the centrifuged sample is separated and located, and an extraction unit to move and receive each component located in the separation unit, ,
The separation unit includes a first separation unit and a second separation unit positioned side by side in one direction and separated by a first partition wall,
The centrifugal separation chamber rotates at an optimum rotation speed or less about a rotation axis,
Assuming that the first inclination angle of the first partition wall is θ1, the average rotation radius of the centrifugal separation chamber is r, the gravitational acceleration is G, and the optimal rotation speed is n,
The optimum rotation speed expressed in revolutions per second is

A method of driving a phosphorus centrifugal separator. According to claim 1,
When the centrifugation chamber is rotated, a second inclination angle of the water surface of the sample positioned in the first separation unit is smaller than or equal to the first inclination angle of the first partition wall. 3. The method of claim 2,
The average rotation radius of the centrifugal separation chamber is a distance from the center of the rotation shaft to the water surface. 4. The method of claim 3,
The maximum value of the average rotation radius of the centrifugal separation chamber is a distance from a center of the rotation shaft to a discharge port located on a bottom surface of the first separation unit. According to claim 1,
The separation unit further includes a third separation unit separated by a second partition wall,
The sample is centrifuged, and the first separation solution is located in the first separation unit, the second separation solution is located in the second separation unit, and the third separation solution is located in the third separation unit. Way. 6. The method of claim 5,
In the initial stage of driving the centrifugation chamber, the centrifugation chamber is rotated faster than the optimal rotation speed to move the first separation solution to the extraction unit, and in the final stage of driving the centrifugation chamber, the centrifugation chamber is A method of driving a centrifugal separation device for moving the first separation solution to the extraction unit by rotating it below the optimum rotation speed. 7. The method of claim 6,
The reference time point for dividing the initial stage and the final stage is a time point at which the first separation liquid stops moving to the extraction unit. 8. The method of claim 7,
In the initial step, a method of driving a centrifugal separation device for moving the first separation liquid to the extraction unit through an outlet located on a bottom surface of the first separation unit adjacent to the first partition wall. According to claim 1,
The sample comprises blood;
A method of driving a centrifugal separation device in which the first separation liquid located in the first separation unit includes plasma. According to claim 1,
The centrifugation chamber may include: an upper plate including an inlet for injecting a sample for centrifugation and an outlet for withdrawing the separated components accommodated in the extraction unit to the outside; and a lower plate sealing the opening in the lower part of the body.

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