KR102425128B1 - Centrifugal device - Google Patents

Abstract

a centrifugal separation unit providing an intermediate chamber into which the centrifugation object is input; and a driving unit disposed on a rotation shaft passing through the intermediate chamber. There is provided a centrifugal separation device comprising a.

Description

Centrifugal device

The present invention relates to a centrifugal separation device, and more particularly, to a centrifugal separation device in which a rotating shaft passes through an intermediate chamber.

Blood may include blood cell components such as red blood cells, white blood cells and platelets, and plasma components composed of water, protein, fat, carbohydrates and other minerals. Such blood may be used as a sample for judging various diseases or health conditions, and is mainly input to a biochip and used to detect the presence or amount of a specific protein in the blood.

Since the protein to be detected is present in the plasma component, the blood cell component may need to be removed from the blood. Therefore, the step of removing the blood cell component from the blood may be preceded before being introduced into the biochip. The amount of plasma in the blood may be about 50-60%.

Separation of blood cells and plasma may be performed by centrifugation. The centrifuge can be used repeatedly in the field in a short time. Since blood comes from the human body, research on a small-capacity centrifuge is being conducted so that it can be detected even with a small amount of blood if possible.

An object of the present invention is to provide a centrifugal separation device capable of simplifying the volume.

An object of the present invention is to provide a centrifugal separation device capable of separating even a small amount of blood.

An object of the present invention is to provide a centrifugal separation device capable of rapidly performing a separation operation.

An object of the present invention is to provide a centrifugal separation device that can be used conveniently.

The problem to be solved by the present invention is to provide an inexpensive centrifugal separation device.

An object of the present invention is to provide a centrifugal separation device capable of performing up to a bioreaction at a time.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, a centrifugal separation apparatus according to an embodiment of the present invention includes a centrifugal separation unit providing an intermediate chamber into which a centrifugal separation object is inserted; and a driving unit disposed on a rotation shaft passing through the intermediate chamber. may include.

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the intermediate chamber may be in the shape of a jar.

In order to achieve the above object, in a centrifugal separation device according to an embodiment of the present invention, the centrifugal separation unit includes a first member and a second member, and the first member is a third member defining an upper side of the intermediate chamber. and one inner surface, and the second member may include a second inner surface defining a lower side of the intermediate chamber.

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the first inner surface may form an acute angle with the axis of rotation, and the second inner surface may form an acute angle with the axis of rotation. .

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the centrifugal separation unit further includes a third member, wherein the third member is disposed between the first member and the second member. A third inner surface connecting the first member and the second member to define an outer chamber communicating with the intermediate chamber may be included.

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the outer chamber may surround a part of the rotation shaft and the intermediate chamber.

In order to achieve the above object, the centrifugal separation device according to an embodiment of the present invention includes a first upper member including the first inner surface and a lower surface defining the outer chamber of the first member A second lower member may be included, and the second member may include a second lower member including the second inner surface and a second upper member including an upper surface defining the outer chamber.

In order to achieve the above object, the centrifugal separation device according to an embodiment of the present invention further includes a connection unit connecting the centrifugal separation unit and the driving unit, and the driving unit may rotate the connection unit and the centrifugal separation unit. .

In order to achieve the above object, the centrifugal separation apparatus according to an embodiment of the present invention includes a centrifugal separator that provides an intermediate chamber into which a centrifugal separation object is introduced, and the centrifugal separator is centered on a rotational shaft passing through the intermediate chamber. can be rotated with

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the intermediate chamber may be in the shape of a jar.

In order to achieve the above object, in a centrifugal separation device according to an embodiment of the present invention, the centrifugal separation unit includes a first member and a second member, and the first member is a third member defining an upper side of the intermediate chamber. and one inner surface, and the second member may include a second inner surface defining a lower side of the intermediate chamber.

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the first inner surface may form an acute angle with the axis of rotation, and the second inner surface may form an acute angle with the axis of rotation. .

In order to achieve the above object, the centrifugal separation device according to an embodiment of the present invention includes a centrifugal separation unit, wherein the centrifugal separation unit includes a first member and a second member, and the first member is the centrifugal separation unit. A first intermediate chamber through which the rotational shaft of the negative part passes through, the second member provides a second intermediate chamber through which the rotational shaft of the centrifugal separator passes, and the first intermediate chamber and the second intermediate chamber communicate with each other. .

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the first member includes a first inner surface defining the first intermediate chamber, and the second member includes the second member and a second inner surface defining an intermediate chamber.

In order to achieve the above object, in the centrifugal separation device according to an embodiment of the present invention, the first inner surface may form an acute angle with the axis of rotation, and the second inner surface may form an acute angle with the axis of rotation. .

Specific details of other embodiments of the present invention are not limited to those mentioned above, and other matters not mentioned will be clearly understood by those skilled in the art from the following description.

According to the centrifugal separation apparatus of the present invention, the volume can be simplified.

According to the centrifugal separation apparatus of the present invention, it may be possible to separate even a small amount of blood.

According to the centrifugal separation apparatus of the present invention, the separation operation can be performed quickly.

According to the centrifugal separation device of the present invention, the operator can conveniently use it in the field.

According to the centrifugal separation apparatus of the present invention, it can be inexpensive.

According to the centrifugal separation apparatus of the present invention, even bioreaction can be performed at once.

The effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 is a cross-sectional view showing a centrifugal separation device according to exemplary embodiments of the present invention.
2 is an exploded cross-sectional view showing a centrifugal separation device according to exemplary embodiments of the present invention.
3 is a cross-sectional view showing a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.
4 is a flowchart illustrating a centrifugal separation method of a centrifugal separation device according to exemplary embodiments of the present invention.
5 to 10 are cross-sectional views illustrating an operating principle of a centrifugal separator of a centrifugal separator according to exemplary embodiments of the present invention.
11 and 12 are cross-sectional views illustrating an operating principle of a centrifuge unit of a centrifugal separation device according to exemplary embodiments of the present invention.
13 is a cross-sectional view illustrating a part of a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.
14 is a cross-sectional view illustrating a part of a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.
15 is a cross-sectional view illustrating a part of a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.
16 is a cross-sectional view showing a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.

In order to fully understand the configuration and effects of the technical idea of the present invention, preferred embodiments of the technical idea of the present invention will be described with reference to the accompanying drawings. However, the technical spirit of the present invention is not limited to the embodiments disclosed below, and may be implemented in various forms and various changes may be made. However, it is provided so that the disclosure of the technical idea of the present invention is complete through the description of the present embodiments, and to fully inform those of ordinary skill in the art to which the present invention belongs, the scope of the invention.

Parts indicated with like reference numerals throughout the specification indicate like elements. Embodiments described in this specification will be described with reference to a block diagram, a perspective view, and/or a cross-sectional view that is an ideal illustration of the technical idea of the present invention. In the drawings, the thickness of the regions is exaggerated for effective description of technical content. Accordingly, the regions illustrated in the drawings have a schematic nature, and the shapes of the illustrated regions in the drawings are intended to illustrate specific shapes of regions of the device and not to limit the scope of the invention. In various embodiments of the present specification, various terms are used to describe various elements, but these elements should not be limited by these terms. These terms are only used to distinguish one component from another. The embodiments described and illustrated herein also include complementary embodiments thereof.

The terminology used herein is for the purpose of describing the embodiments and is not intended to limit the present invention. In this specification, the singular also includes the plural, unless specifically stated otherwise in the phrase. As used herein, 'comprises' and/or 'comprising' does not exclude the presence or addition of one or more other elements.

Hereinafter, the present invention will be described in detail by describing preferred embodiments of the technical idea of the present invention with reference to the accompanying drawings.

1 is a cross-sectional view illustrating a centrifugal separation device according to exemplary embodiments of the present invention, and FIG. 2 is an exploded cross-sectional view illustrating a centrifugal separation device according to exemplary embodiments of the present invention.

Hereinafter, the right direction of FIG. 1 is the first direction (D1), the upward direction is the second direction (D2), the direction substantially perpendicular to the first direction (D1) and the second direction (D2) and the direction facing forward It may be referred to as a three-way (D3).

1 and 2 , the centrifugal separation device H may include a centrifugal separation unit 1 , a driving unit 5 , and a connecting unit 3 . An intermediate chamber C and an outer chamber O may be provided in the centrifuge unit 1 . The intermediate chamber (C) may be located in the middle of the centrifugation unit (1). The intermediate chamber C may include a first intermediate chamber C1 (refer to FIG. 3 ) and a second intermediate chamber C2 (refer to FIG. 3 ). The intermediate chamber (C) may be in the shape of a jar. The outer chamber (O) may surround the intermediate chamber (C). In embodiments, the volume of the outer chamber O may be 40-50% of the volume of the centrifugation object (B, see FIG. 6 ). However, the present invention is not limited thereto, and a mechanism capable of adjusting the volume of the outer chamber O may be included. The driving unit 5 may rotate the centrifugal separation unit 1 . In embodiments, the driving unit 5 may include a motor or the like. The driving unit 5 may include a driving body 51 and a driving shaft 53 . The driving body 51 may rotate the driving shaft 53 . The drive shaft 53 may be rotated by being coupled to the centrifugal separator 1 via the connecting part 3 . The connecting part 3 may connect the centrifugal separator 1 and the driving part 5 . The connection part 3 may include a centrifugal separator connection part 31 and a drive shaft connection hole 33 . The centrifugal separator connection part 31 may be depressed in a direction opposite to the second direction D2. The centrifuge part 1 may be inserted into the centrifuge part connection part 31 . The drive shaft connection hole 33 may be depressed in the second direction D2 . The drive shaft 53 may be inserted into the drive shaft connection hole 33 .

3 is a cross-sectional view showing a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Referring to FIG. 3 , the centrifugal separator 1 may include a first member 11 , a second member 13 , and a third member 15 .

In embodiments, the first member 11 may have a symmetrical shape with respect to the rotation axis (X). The rotation axis X may be substantially parallel to the second direction D2. The first member 11 may include a first lower member 111 and a first upper member 113 . The first lower member 111 may be deployed on a plane defined in the first direction D1 and the third direction D3 . The first lower member 111 may have a donut shape. The first upper member 113 may extend in the second direction D2 from the first lower member 111 . The height of the first upper member 113 may be h1. The first upper member 113 may have a cut cone shape whose cross-sectional area becomes narrower toward the second direction D2. The first upper member 113 and the first direction D1 may form a predetermined angle a. a may be 0 degrees or more and 90 degrees or less. More specifically, a may be greater than 0 degrees and less than 90 degrees. More preferably, a may be 45 to 80 degrees. The interior of the first upper member 113 may be empty. A first intermediate chamber C1 may be provided inside the first upper member 113 . The first intermediate chamber C1 may be defined by the first inner surface 113h. The first inner surface 113h may be hydrophobically treated. A configuration such as a protrusion (not shown) may be additionally provided on the first inner surface 113h. The upper end of the first upper member 113 may be open. The first intermediate chamber C1 may communicate with the outside through the inlet 11h. A sealing member (not shown) for blocking the inlet 11h may be further provided. In embodiments, the first member 11 may include a transparent material. The first member 11 may include plastic. In embodiments, the first member 11 may be disposable.

The second member 13 may be spaced apart from the first member 11 in the second direction D2 . In embodiments, the second member 13 may have a symmetrical shape with respect to the rotation axis (X). The rotation axis X may be substantially parallel to the second direction D2. The second member 13 may include a second upper member 131 , a second lower member 133 , and a support member 135 . The second upper member 131 may be deployed on a plane defined in the first direction D1 and the third direction D3 . The second upper member 131 may have a donut shape. The second lower member 133 may extend in a direction opposite to the second direction D2 from the second upper member 131 . The height of the second lower member 133 may be h2. The second lower member 133 may have a cut cone shape in which the cross-sectional area becomes narrower toward the direction opposite to the second direction D2. The second lower member 133 and the first direction D1 may form a predetermined angle b. b may be 0 degrees or more and 90 degrees or less. More specifically, b may be greater than 0 degrees and less than 90 degrees. More preferably, b may be 45 to 80 degrees. The inside of the second lower member 133 may be empty. A second intermediate chamber C2 may be provided inside the second lower member 133 . The second intermediate chamber C2 may be defined by the second inner surface 133h. The second inner surface 133h may be hydrophobically treated. A configuration such as a protrusion (not shown) may be additionally provided on the second inner surface 133h. The support member 135 may be coupled under the second lower member 133 . The second member 13 may include plastic. In embodiments, the second member 13 may be disposable.

The rotation axis (X) may pass through the intermediate chamber (C). In a portion where the first intermediate chamber C1 and the second intermediate chamber C2 meet, the radius of the intermediate chamber C with the rotation axis X as the center may be r1.

The third member 15 may be positioned between the first member 11 and the second member 13 . In embodiments, the third member 15 may have a donut shape. The third member 13 can join the first member 11 and the second member 13 together. In embodiments, the third member 15 may include double-sided tape, silicone, rubber, or the like. The height of the third member 15 may be h3. In embodiments, h3 may be less than h1 and h2. The distance between the third member 15 and the rotation axis X may be r2. r2 may be greater than r1. The outer chamber O may be defined by the third inner surface 15h of the third member 15 and the lower surface of the first lower member 111 and the upper surface of the second upper member 131 . The outer chamber O may communicate with the intermediate chamber C.

4 is a flowchart illustrating a centrifugal separation method of a centrifugal separation device according to exemplary embodiments of the present invention.

Referring to FIG. 4 , the centrifugation method (S) of the centrifugal separation device includes inputting a centrifugation object into the intermediate chamber (S1), rotating the centrifugation device (S2), and extracting from the intermediate chamber (S3) ) may be included. Hereinafter, each step of the centrifugal separation method (S) will be described in detail with reference to FIGS. 5 to 10 .

5 to 10 are cross-sectional views illustrating the principle of operation of a centrifuge unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Referring to FIG. 5 , the centrifugation object B may be introduced into the centrifugation unit 1 through the inlet 11h. The input of the centrifugation object (B) may be performed by a pipette or the like. The centrifugal separation object (B) may fall in a direction opposite to the second direction (D2). The centrifugal separation object (B) may be moved by gravity acting in a direction opposite to the second direction (D2). The centrifugation object (B) may include two or more materials having different densities. In embodiments, the object to be centrifuged (B) may contain blood. However, the present invention is not limited thereto, and may include other liquids.

Referring to FIG. 6 , the centrifugation target (B) may be located in the intermediate chamber (C, see FIG. 3 ) of the centrifugation unit 1 . The centrifugal separation object (B) may be in contact with the first inner surface (113h) and the second inner surface (133h). Depending on the amount of the object to be centrifuged (B), a part of the object to be centrifuged (B) may be located in the outer chamber (O).

Referring to FIG. 7 , the centrifugation object (B) may rotate. More specifically, the centrifugation object B may be rotated by the driving unit 5 (see FIGS. 1 and 2 ). The centrifugal separation object (B) may rotate based on the rotation axis (X). The centrifugation object (B) may rotate at a constant speed. The centrifugation object (B) may rotate at a speed of V1. When the object to be centrifuged (B) rotates at a speed of V1, a portion of the object to be centrifuged may move in the first direction (D1) and in directions opposite to the first direction (D1). A part of the centrifugation object may be further moved to the outer chamber (O). The portion remaining in the intermediate chamber may be referred to as a first portion to be centrifuged B1 , and the portion entering the outer chamber may be referred to as a second portion to be centrifuged B2 . In embodiments, the second part B2 to be centrifuged may come into contact with the third inner surface 15h of the third member 15 . The centrifugation target may no longer be located in the center of the intermediate chamber. The center of the intermediate chamber may be emptied. The centrifugal separation target may not exist in the portion through which the rotational axis X passes.

Referring to FIG. 8 , the centrifugal separator 1 may rotate at a speed of V2. In embodiments, V2 may be greater than V1. Therefore, the centrifugal separator 1 can rotate faster. The centrifugation object may be separated. The material having a high density may be biased toward the third inner surface 13h away from the rotation axis X. The separated material having a high density may be referred to as a second material M2. A material having a low density may be distributed relatively close to the rotation axis (X). The separated material having a low density may be referred to as a first material M1. In embodiments, when the object to be centrifuged is blood, the first material M1 may include plasma. The second material M2 may include blood cells. That is, plasma and blood cells can be separated by rotation. The rotation of the centrifuge unit 1 may be continued until plasma and blood cells are properly separated. When the first member 11 is transparent, the separation of plasma and blood cells may be visually confirmed. Alternatively, a separate imaging device (not shown) may be further included to detect the separation of plasma and blood cells.

Referring to FIG. 9 , the rotation of the centrifugal separator 1 may be stopped. The first material M1 may be collected on the support member 135 by gravity. The second material M2 may be continuously positioned in the outer chamber. Depending on the amount of blood or the proportion of blood cells in the blood, a portion of the first material M1 ′ may remain in the outer chamber.

Referring to FIG. 10 , the first material M1 collected in the intermediate chamber may be extracted. More specifically, the first material M1 collected on the support member 135 through the inlet 11h may be extracted to the outside. Extraction may be performed by a pipette or the like. The extracted first material M1 may be used for various purposes. In embodiments, when the object to be centrifuged (B) is blood, the first material M1 including plasma may be used to perform a bioreaction. When the volume of the outer chamber O is greater than 40-50% of the combined volume of the outer chamber O and the intermediate chamber C, the extracted material may include only plasma without blood cells. Therefore, the accuracy of the bioreaction can be improved.

According to the centrifugal separation device according to the exemplary embodiments of the present invention, since it is rotated based on the rotation axis passing through the intermediate chamber, the volume of the centrifugal separation device can be reduced, that is, the centrifugal separation object is inserted into the chamber spaced apart from the rotation center. Rather than being done, since the centrifugation object is introduced into the intermediate chamber through which the rotating shaft passes, and centrifugation is performed, the volume of the apparatus can be reduced. The reduced volume centrifugal separation device can be easily used in the field and can be inexpensive. In addition, the amount of centrifugation target required for separation can be reduced. When blood is to be separated, only a small amount of blood needs to be extracted, thereby reducing the burden on the human body. Furthermore, since separation is possible within a short time, work efficiency can be improved.

11 and 12 are cross-sectional views illustrating an operating principle of a centrifuge unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Hereinafter, contents substantially the same as or similar to those described with reference to FIGS. 1 to 10 may be omitted for convenience.

Referring to FIG. 11 , in the state of FIG. 8 , the centrifugal separation object B3 may be further introduced into the intermediate chamber through which the rotation shaft X passes.

Referring to FIG. 10 , the centrifugal separator 1 may be rotated at a speed of V3. V3 may be greater than V1. The centrifugal separator 1 may be further rotated to separate the first material M1 ′ and the second material M2 ′. Thereafter, as shown in FIG. 10 , the first material M1 ′ may be extracted through the inlet 11h.

The centrifugation unit 1 is rotated at a constant speed V1 to form an empty space in the central chamber through which the rotation shaft X passes, and the centrifugation object B3 is further introduced to further rotate at the speed of V3, so that more first A material (M1') can be obtained. The accuracy of the analysis can be improved.

13 is a cross-sectional view illustrating a part of a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Hereinafter, content substantially the same as or similar to that described with reference to FIGS. 1 to 12 may be omitted for convenience.

Referring to FIG. 13 , a microstructure 17 may be further provided on the upper surface of the second upper member 131 . The microstructure 17 may be disposed close to the central chamber. The microstructure 17 may have a rectangular cross-section. The microstructure 17 may prevent the first material and the second material from being mixed after the first material and the second material are separated. When the first material flows down due to gravity, the second material may be prevented from flowing down with the microstructure 17 . The efficiency of centrifugation can be improved.

14 is a cross-sectional view illustrating a part of a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Hereinafter, contents substantially the same as or similar to those described with reference to FIGS. 1 to 13 may be omitted for convenience.

Referring to FIG. 14 , a microstructure 17 ′ may be further provided on the upper surface of the second upper member 131 . The microstructure 17 ′ may have a cross-sectional cross-section in which a triangular shape is arranged in the second direction D2 . The microstructure 17 ′ may prevent the first material and the second material from being mixed after the first material and the second material are separated. When the first material flows down due to gravity, the second material may be prevented from flowing down with the microstructure 17'. The efficiency of centrifugation can be improved.

15 is a cross-sectional view illustrating a part of a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Hereinafter, contents substantially the same as or similar to those described with reference to FIGS. 1 to 14 may be omitted for convenience.

Referring to FIG. 15 , a microstructure 17 ″ may be further provided on the upper surface of the second upper member 131 . The microstructure 17 ″ may increase in height from the third member 15 in the second direction D2 . The microstructure 17 ″ may prevent the first material and the second material from being mixed after the first material and the second material are separated. When the first material flows down due to gravity, the second material flows down together can be prevented by the microstructure 17 ″. The efficiency of centrifugation can be improved.

16 is a cross-sectional view showing a centrifugal separation unit of a centrifugal separation device according to exemplary embodiments of the present invention.

Hereinafter, content substantially the same as or similar to that described with reference to FIGS. 1 to 15 may be omitted for convenience.

Referring to FIG. 16 , the centrifuge unit 1 may further include a biosensor 17 . The biosensor 17 may be coupled to the second inner surface 133h. When the first material flows down and comes into contact with the biosensor 17 , a bioreaction may be performed in the biosensor 17 . The bioreaction may include an antigen-antibody reaction and the like. In embodiments, while the bioreaction is being performed, the centrifuge unit 1 may rotate.

In embodiments, the support member 135 ′ may include a valve. The valve can be opened. By opening the valve, the first material for which the bioreaction is completed may be discharged downward.

According to the centrifugal separation apparatus according to exemplary embodiments of the present invention, the biosensor 17 is present in the centrifugal separation unit 1 to perform a bioreaction immediately after centrifugation. Therefore, the reaction rate can be improved. A separate process for extracting the separated material through the inlet 11h may not be required. Therefore, the whole process can be simplified, and the operator can work conveniently. Furthermore, since a separate biosensor is not required, the volume of the equipment may be reduced. That is, a centrifugal separator can be used as a reaction vessel for a bioreaction.

As mentioned above, although embodiments of the present invention have been described with reference to the accompanying drawings, those of ordinary skill in the art to which the present invention pertains can implement the present invention in other specific forms without changing its technical spirit or essential features. You will understand that there is Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

H: centrifugation device
1: Centrifuge
11: first member
11h: Entrance
13: second member
15 third member
C: middle chamber
O: outer chamber
3: Connection
5: drive
51: drive body
53: drive shaft
B: centrifugation object
M1: first substance
M2: second substance

Claims (17)

a centrifugal separation unit providing an intermediate chamber into which the centrifugation object is input; and
a driving unit disposed on a rotation shaft passing through the intermediate chamber; including,
The centrifugal separator includes a first member, a second member and a third member,
the first member comprises a first inner surface defining an upper side of the intermediate chamber;
wherein the second member comprises a second inner surface defining a lower side of the intermediate chamber;
The third member includes a third inner surface connecting the first member and the second member between the first member and the second member, the third inner surface defining an outer chamber communicating with the intermediate chamber;
The first inner surface forms an acute angle with the axis of rotation,
The second inner surface forms an acute angle with the axis of rotation,
The outer chamber surrounds a portion of the rotation shaft and the intermediate chamber,
and a microstructure coupled to the second member is provided in the outer chamber.
delete delete delete delete delete The method of claim 1,
The first member includes a first upper member including the first inner surface and a second lower member including a lower surface defining the outer chamber,
wherein the second member includes a second lower member including the second inner surface and a second upper member including an upper surface defining the outer chamber.
The method of claim 1,
It further comprises a connection part for connecting the centrifugal separator and the driving part,
The driving unit is a centrifugal separation device for rotating the connecting portion and the centrifugal separation unit.
delete delete delete delete including a centrifuge,
The centrifugal separator includes a first member and a second member,
The first member provides a first intermediate chamber through which the rotation shaft of the centrifugal separator passes,
The second member provides a second intermediate chamber through which the rotation shaft of the centrifugal separator passes,
The first intermediate chamber and the second intermediate chamber are communicated,
the first member comprises a first inner surface defining the first intermediate chamber;
the second member comprises a second inner surface defining the second intermediate chamber;
The first inner surface forms an acute angle with the axis of rotation,
The second inner surface forms an acute angle with the axis of rotation,
The centrifugal separation unit further comprises a biosensor coupled to the second inner surface.
delete delete delete 14. The method of claim 13,
The centrifugal separation unit further comprises a support member provided with a valve that opens downward.

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