KR102404000B1 - Chamber for centrifuge device and centrifuge device comprising the same - Google Patents

Abstract

The present embodiments relate to a chamber for a centrifugal separation device and a centrifugal separation device including the same. A chamber for a centrifugal separation device according to an embodiment includes a main body, an upper plate, and a lower plate, wherein the main body includes first to third separation units in which each component of a centrifuged sample is separated and located, the first a first extraction unit for moving and accommodating components located in the separation unit; a second extraction unit for further separating the components accommodated in the first extraction unit and then moving and accommodating; and a third extraction unit for moving and accommodating components located in the second separation unit, wherein the upper plate includes: an inlet for injecting a sample for centrifugal separation; and a first outlet and a second outlet for drawing out the separated components accommodated in the second and third extracting units, respectively, and the lower plate may seal the opening of the lower part of the main body.

Description

Chamber for centrifugal separation device and centrifugal separation device comprising same

The present embodiment relates to a chamber for a centrifugal separation device and a centrifugal separation device including the same. More specifically, it relates to a chamber for a centrifugal separator capable of easily extracting a sample separated by component through each outlet without contamination to the outside of the chamber, and a centrifugal separator including the same.

Whole blood or various biological fluids may be separated from its components or parts. For example, whole blood is composed of plasma, white blood cells, platelets, and red blood cells, and may be separated for each component in a centrifugal separation device or the like.

In the prior art, for example, in order to separate whole blood for each component, whole blood was put into a single tube container and then put into a centrifugal separator to separate based on specific gravity. The centrifuged sample was stacked in several layers for each component based on specific gravity, and each component layered and stacked in this way was manually recovered.

However, in general, when each layer of a sample stacked for each component is separated by hand, work efficiency is remarkably reduced, and each centrifuged component is mixed again, and there is no choice but to extract a specific component. However, there was a limit to complete extraction. In addition, when various samples are simultaneously processed in a narrow space, there is a problem in that cross-contamination between different samples often occurs.

Therefore, when a biological fluid such as whole blood is separated using a centrifugal separation device, it is urgent to develop a technology capable of easily extracting a desired component without re-contamination in the extraction process after separating the fluid for each component.

In one embodiment, it includes a main body, an upper plate, and a lower plate, wherein the main body comprises first to third separation units in which each component of the centrifuged sample is separated, and components located in the first separation unit. a first extraction unit to move and accommodate; a second extraction unit for further separating the components accommodated in the first extraction unit and then moving and accommodating; and a third extraction unit for moving and accommodating components located in the second separation unit, wherein the upper plate includes: an inlet for injecting a sample for centrifugal separation; and a first outlet and a second outlet for withdrawing the separated components accommodated in the second and third extracting units to the outside, respectively, wherein the lower plate closes the opening at the lower part of the main body. It is intended to provide a chamber for the device.

In another embodiment, it includes a main body, an upper plate, and a lower plate, wherein the main body, each component of the centrifuged sample is separated from the first to third separation units located; a first extraction unit for moving and accommodating components located in the first separation unit; a second extraction unit for further separating the components accommodated in the first extraction unit and then moving and accommodating; and a third extraction unit for moving and receiving each component positioned in the second separation unit, wherein the upper plate includes: a first inlet for injecting a sample for centrifugal separation; a second inlet for injecting the Ficoll solution; and a first outlet and a second outlet for withdrawing the separated components accommodated in the second and third extracting units to the outside, respectively, wherein the lower plate closes the opening at the lower part of the main body. It is intended to provide a chamber for the device.

In another embodiment, to provide a centrifugal separation device including at least two chambers for the centrifugal separation device according to the embodiment.

An object of the present embodiment is to provide a chamber for a centrifugal separation device that can be easily extracted without recontamination in the process of extracting a desired component after centrifugation of a biological fluid, and a centrifugal separation device including the same.

Since the chamber for a centrifugal separation device according to an embodiment includes an extraction unit for moving and accommodating the separated components in the separation unit together with the separation unit in which the centrifuged components are separated, centrifugal separation after centrifugation of the biological fluid It can be easily separated without re-contamination in the process of extracting the separated components.

In addition, in the chamber for the centrifugal separation device according to the present embodiment, since the outlet included in the upper plate includes a stopper including a cutout that passes gas but does not pass liquid, the sample separated in the process of extracting the centrifuged component to prevent leakage or contamination.

1 is a plan view schematically illustrating a chamber for a centrifugal separation device according to an embodiment.
FIG. 2 is an exploded perspective view of the chamber for the centrifugal separation device of FIG. 1 .
3A is a cross-sectional view of the centrifugal separation device chamber of FIG. 1 taken along line A-A', and FIGS. 3B and 3C are plan and cross-sectional views of the first outlet stopper, respectively.
4A is a cross-sectional view of the centrifugal separation device chamber of FIG. 1 taken along line B-B', and FIGS. 4B and 4C are plan and cross-sectional views of the second outlet stopper, respectively.
5 is a cross-sectional view taken along line I-II of the chamber for the centrifugal separation device of FIG. 1 .
6 is a plan view schematically illustrating a chamber for a centrifugal separation device according to another embodiment.
7 is an exploded perspective view of the chamber for the centrifugal separation device of FIG. 6 .
8A is a cross-sectional view of the centrifugal separation device chamber of FIG. 6 taken along line A-A', and FIGS. 8B and 8C are plan and cross-sectional views of the first outlet stopper, respectively.
9A is a cross-sectional view of the centrifugal separation device chamber of FIG. 6 taken along line B-B', and FIGS. 9B and 9C are plan and cross-sectional views of the second outlet stopper, respectively.
10 is a cross-sectional view taken along line I-II of the chamber for the centrifugal separation device of FIG. 6 .
11 is a plan view schematically illustrating a centrifugal separation device according to an embodiment.

Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and the same reference numerals are given to the same or similar components throughout the specification.

In addition, since the size and thickness of each component shown in the drawings are arbitrarily indicated for convenience of description, the present invention is not necessarily limited to the illustrated bar. In order to clearly express various layers and regions in the drawings, the thicknesses are enlarged. And in the drawings, for convenience of description, the thickness of some layers and regions are exaggerated.

Further, when a part of a layer, film, region, plate, etc. is said to be “on” or “on” another part, it includes not only cases where it is “directly on” another part, but also cases where another part is in between. . Conversely, when we say that a part is "just above" another part, we mean that there is no other part in the middle. In addition, to be "on" or "on" the reference part is located above or below the reference part, and does not necessarily mean to be located "on" or "on" the opposite direction of gravity. .

In addition, throughout the specification, when a part "includes" a certain component, this means that other components may be further included, rather than excluding other components, unless otherwise stated.

In addition, throughout the specification, when referring to "planar", it means when the target part is viewed from above, and "cross-sectional" means when viewed from the side when a cross-section of the target part is vertically cut.

1 is a plan view schematically showing a chamber for a centrifugal separation device according to an embodiment, and FIG. 2 is an exploded perspective view of the chamber for a centrifugal separation device of FIG. 1 .

Hereinafter, a chamber for a centrifugal separation device according to an embodiment will be described in detail with reference to FIGS. 1 and 2 .

1 and 2 , the chamber 500 for a centrifugal separation device according to an embodiment includes an upper plate 100 , a body 200 , and a lower plate 300 .

The body 200 includes a separation unit 210 in which the centrifuged sample is separated for each component and an extraction unit 220 to move and receive the sample separated by component in the separation unit 210 .

First, the separation unit 210 includes a first separation unit 211 , a second separation unit 212 , and a third separation unit 213 positioned side by side in one direction.

At this time, each region of the first separation unit 211 and the second separation unit 212 is separated by a first partition wall 311 positioned in the separation unit 210 , and the second separation unit 212 and the third separation unit 212 are separated from each other. Each region of the separation unit 213 is separated by a second partition wall 312 positioned in the separation unit 210 .

As described above, since the separation unit 210 includes the first separation unit 211 , the second separation unit 212 , and the third separation unit 213 , each component of the centrifuged sample is first separated for each component. The unit 211 , the second separation unit 212 , and the third separation unit 213 may be respectively located.

Meanwhile, the main body 200 includes an extraction unit 220 .

The extraction unit 220 includes a first extraction unit 221 , a second extraction unit 222 , and a third extraction unit 223 .

More specifically, the first extraction unit 221 is a space for moving and receiving the components separated by the first separation unit 211 , and the second extraction unit 222 is the component moved to the first extraction unit 221 . It is a space to move and accommodate the additionally separated components after additional separation. The third extraction unit 223 is a space for moving and accommodating components located in the second separation unit.

The first separation unit 211 is connected to the first extraction path 321 located at the lower portion of the main body 200 . The components separated by the first separation unit 211 after centrifugation are moved through the first extraction path 321 connected to the first separation unit 211 and accommodated in the first extraction unit 221 .

The first extraction unit 221 is connected to the second extraction path 322 located at the lower portion of the main body 200 . The components accommodated in the first extraction unit 221 are further separated by a method of performing centrifugation once more, are moved through the second extraction path 322 and are accommodated in the second extraction unit 222 . In this case, a high-purity sample can be obtained by more reliably removing the contaminants included in the components separated by the first separation unit 211 after centrifugation.

When, for example, blood is injected as a sample, the separated component positioned in the first separator 211 through centrifugation is plasma.

Such plasma is used for genomic analysis of cell free DNA, which is used as a method of liquid biopsy to perform a diagnosis using a part of a specific tissue secreted into blood without directly extracting the patient's tissue. That is, in the genomic analysis of free DNA, a method of increasing the accuracy of diagnosis by centrifuging the plasma obtained after separating the components of whole blood twice to remove contaminants is common. In this case, it is necessary to manually divide the components of the blood by changing several tubes, and to centrifuge them continuously again. cause the rise.

However, as in the present embodiment, after plasma is primarily separated in the first extraction unit 221 and additional centrifugation is performed, the supernatant separated in the first extraction unit 221 is transferred to the second extraction unit 222 . By accommodating it, it is possible to obtain plasma that is not contaminated by other DNA components or the like.

The first extraction part 221 includes a second vent hole extension part 252 on a sealed upper surface, and the second vent hole extension part 252 has a second vent hole 132 located in the upper plate 100 . ) and is a unitary structure. The second vent hole 132 is formed when the component separated by the first separation unit 211 is moved to the first extraction unit 221 , that is, when the first valve is opened, the second vent hole 132 is also should be open In addition, when the component additionally separated by the first extraction unit 221 is moved to the second extraction unit 222 , that is, when the second valve is opened, the second vent hole 132 must also be opened. When the first valve is closed, the second vent hole 132 may also be closed.

Meanwhile, the second extraction unit 222 includes the first extraction unit 241 .

The first draw-out part 241 is connected to the first draw-out hole 121 positioned on the upper plate 100 .

3A is a cross-sectional view of the centrifugal separation device chamber of FIG. 1 taken along line A-A', and FIGS. 3B and 3C are a plan view and a cross-sectional view of the first outlet stopper, respectively.

Referring to FIG. 3A , the lower end of the first outlet 121 includes a first outlet stopper 121a for sealing the inner peripheral lower surface of the first outlet 121 . That is, the first outlet stopper 121a is opened only when pressed with a withdrawal tool for withdrawing the centrifuged component to the outside, and is normally closed. Therefore, except when withdrawing the components separated by the second extraction unit 222 to the outside, the separated components are prevented from popping out due to unintended circumstances (eg, when the centrifugal separation chamber is overturned), and Incorporation and contamination of pollutants in the air can be prevented.

Referring to FIG. 3A , the first lead-out part 241 has a shape in which the upper and lower surfaces of the main body 200 are open.

In this case, a portion of the wall of the first lead-out part 241 may be formed in a structure having an inclination in the thickness direction. When the first extraction unit 241 includes a slope, the components accommodated in the second extraction unit 222 can be quickly withdrawn, and a desired sample can be well recovered without omission. That is, the first lead-out portion 241 only needs to include an inclination in the thickness direction, and does not have to be formed in a special angle range. Accordingly, the first lead-out part 241 may be configured in a conical shape, for example, in which the upper part has a larger diameter than the lower part.

Next, the average thickness of the first outlet stopper 121a may be in the range of 0.1 mm to 10 mm, more specifically, in the range of 0.3 mm to 5 mm, or in the range of 0.5 mm to 3 mm. When the thickness of the first outlet stopper 121a satisfies the above range, a pull-out tool for withdrawing the additional centrifuged components accommodated in the second extracting unit 222 can be easily inserted into the first withdrawing unit 241. and it is possible to effectively prevent the components of the sample separated as described above from popping out due to unintended circumstances.

In addition, the first outlet stopper 121a may be made of a material having elasticity, for example, it may be formed of an elastic material such as rubber, silicone, or a polymer compound.

Referring to FIGS. 3A to 3C , the area of the first outlet stopper 121a in a plan view is larger than that of the first outlet stopper 121 . Accordingly, the first outlet stopper 121a may be positioned at the lower end of the first outlet 121 to seal the inner circumferential lower surface of the first outlet 121 and seal the upper surface of the first outlet 241 at the same time.

The first outlet stopper 121a is a first cutout 121aa formed so that a withdrawal tool for withdrawing the additional centrifuged component accommodated in the second extraction unit 222 can be inserted into the first extraction unit 241 . ) is included.

The first cutout 121aa may have, for example, at least one of a straight line shape, a cross shape, and an asterisk shape. The shape of the first cutout 121aa is not particularly limited as long as a tool, for example, a pipette, for extracting the components accommodated in the second extraction unit 222 by further centrifugation can be inserted thereinto.

In the present embodiment, the first cutout 121aa prevents a liquid having a high surface tension from passing through the narrow gap of the cutout, and gas such as air or air bubbles can pass therethrough. Accordingly, even if the centrifugation chamber 500 is turned over during the sample withdrawal process after centrifugation, it is possible to prevent leakage of the liquid component of the separated sample, which is very useful. In addition, when the component accommodated in the first extraction unit 221 is moved to the second extraction unit 222 through the second extraction path 322, it sufficiently serves as air circulation without a separate vent hole, so that the first vent hole ( 131) or the second vent hole 132, there is an advantage that a separate additional vent hole is not required.

The average spacing of the first cutouts 121aa may be in the range of 0.01 mm to 2 mm, more specifically, in the range of 0.05 mm to 1 mm or 0.1 mm to 0.5 mm. When the average interval of the first cutouts 121aa satisfies the above range, it is possible to prevent the separated sample from leaking out even when the centrifugal separation chamber 500 is overturned, and the first extraction unit 221 ) when moving the component accommodated in the second extraction passage 322 to the second extraction unit 222, even if there is no separate vent hole, it can serve as an air circulation.

Next, the average length of the first cutout 121aa may be in the range of 0.01 mm to 10 mm, more specifically, in the range of 0.05 mm to 8 mm or 0.1 mm to 6 mm. When the average length of the first cut-out portion 121aa satisfies the above range, an extraction tool for withdrawing the additional centrifuged component accommodated in the second extraction portion 222 is easily inserted into the first extraction portion 241. can do.

In this way, the components further separated and accommodated in the second extraction unit 222 are inserted into the first extraction unit 241 through the first cut-out portion 121aa of the first exit port 121 and the first exit stopper 121a. It can be withdrawn to the outside by using a withdrawal tool. In this case, the withdrawal to the outside through the first outlet 121 may be performed using, for example, a pipette.

Next, the second separation unit 212 is connected to the third extraction path 323 located in the lower portion of the main body (200). The components separated by the second separation unit 212 after centrifugation are moved through the third extraction path 323 connected to the second separation unit 212 and accommodated in the third extraction unit 223 .

The body 200 includes a third valve unit 233 positioned to overlap a partial region of the third extraction path 323 . At this time, a third valve is positioned in the third valve unit 233 , and the third extraction path 323 is opened and closed by the third valve. Therefore, in order to extract the components separated by the second separator 212 after centrifugation, the third valve is driven to open the third extraction passage 323 to remove the separated components in the second separator 212 . 3 It can be moved to the extraction unit 223 .

The third extractor 223 includes a second extractor 242 .

The second draw-out part 242 is connected to the second draw-out hole 122 positioned on the upper plate 100 .

4A is a cross-sectional view of the centrifugal separation device chamber of FIG. 1 taken along line B-B', and FIGS. 4B and 4C are plan and cross-sectional views of the second outlet stopper, respectively.

Referring to FIG. 4A , the second outlet 122 includes a second outlet stopper 122a for sealing the inner circumferential lower surface of the second outlet 122 . That is, the second outlet stopper 122a is opened only when pressed with a withdrawal tool for withdrawing the centrifuged component to the outside, and is normally closed. Therefore, it is possible to prevent the separated components from popping out due to unintended circumstances, except when withdrawing the components separated by the third extraction unit 223 to the outside, and to prevent mixing and contamination of contaminants in the outside air. have.

Referring to FIG. 4A , the second lead-out part 242 has an open upper surface and a lower surface of the main body 200 .

In this case, a portion of the wall of the second lead-out part 242 may be formed to have a structure having an inclination in the thickness direction. When the second extraction unit 242 includes a slope, the components accommodated in the third extraction unit 223 can be quickly withdrawn, and a desired sample can be well recovered without omission. That is, the second lead-out part 242 only needs to include an inclination in the thickness direction, and does not have to be formed in a special angle range. Accordingly, the second lead-out part 242 may be configured in a conical shape, for example, in which the upper part has a larger diameter than the lower part.

Next, the average thickness of the second outlet stopper 122a may be in the range of 0.1 mm to 10 mm, more specifically, in the range of 0.3 mm to 5 mm, or in the range of 0.5 mm to 3 mm. When the thickness of the second draw-out stopper 122a satisfies the above range, a draw-out tool for drawing out the components contained in the third extracting unit 223 can be easily inserted into the second draw-out unit 242 .

In addition, the second outlet stopper 122a may be made of a material having elasticity, for example, it may be formed of an elastic material such as rubber, silicone, or a polymer compound.

4A to 4C , the area of the second outlet stopper 122a in a plan view is larger than that of the second outlet stopper 122 . Accordingly, the second outlet stopper 122a may be positioned at the lower end of the second outlet 122 to seal the inner circumferential lower surface of the second outlet 122 and seal the upper surface of the second outlet 242 at the same time.

The second outlet stopper 122a is a second cutout 122aa formed so that a withdrawal tool for withdrawing the centrifuged component accommodated in the third extraction unit 223 can be inserted into the second extraction unit 242 . includes

The second cutout 122aa may have, for example, at least one of a straight line shape, a cross shape, and an asterisk shape. The shape of the second cut-out 122aa is not particularly limited as long as it is centrifuged and a tool for extracting the components contained in the third extraction unit 223, for example, a tip of a pipette can be inserted.

In this embodiment, the second cut-out 122aa may not pass a liquid having a high surface tension, but may pass a gas that does not. Accordingly, it is very useful to prevent the separated sample from leaking even if the centrifugation chamber 500 is turned over during the sample withdrawal process after centrifugation. In addition, when the components separated by the second separation unit 212 are moved to the third extraction unit 223 through the third extraction path 323, the first vent hole 131 serves to circulate air without a separate vent hole. ) or a second vent hole 132, there is an advantage that a separate vent hole is not required.

The average spacing of the second cutouts 122aa may be in the range of 0.01 mm to 2 mm, more specifically, in the range of 0.05 mm to 1 mm or 0.1 mm to 0.5 mm. When the average interval of the second cut-outs 122aa satisfies the above range, it is possible to prevent the separated sample from leaking out even when the centrifugal separation chamber 500 is turned over, and the second separation unit 212 ) may serve as air circulation without a separate vent hole when moving the component accommodated in the third extraction path 323 to the third extraction unit 223 .

Next, the average length of the second cutout 122aa may be in the range of 0.01 mm to 10 mm, more specifically, in the range of 0.05 mm to 8 mm or 0.1 mm to 6 mm. When the average length of the second cut-out portion 122aa satisfies the above range, an extraction tool for withdrawing the additional centrifuged component accommodated in the third extraction portion 223 is easily inserted into the second extraction portion 242. can do.

After being centrifuged in this way, the components accommodated in the third extraction unit 223 are removed using a withdrawal tool inserted into the second extraction unit 242 through the second exit port 122 and the second exit stopper 122a. can be withdrawn with In this case, the withdrawal to the outside through the second outlet 122 may be performed using, for example, a pipette.

Next, the separation unit 210 will be described in more detail.

As described above, the separation unit 210 includes a first separation unit 211 , a second separation unit 212 , and a third separation unit 213 , and includes a first partition wall 311 and a second partition wall 312 . ) to separate each area.

5 is a cross-sectional view taken along line I-II of the chamber for the centrifugal separation device of FIG. 1 .

Referring to FIG. 5 , the first partition wall 311 may be formed to have a predetermined distance H1 from the upper plate 100 . Specifically, the first partition wall 311 and the upper plate 100 may be formed to have a height spaced apart from each other by 0.1 mm to 2 mm, for example. Specifically, since the height of the first partition wall 311 is slightly lower than the height of the main body 200 , it has a structure in which the first partition wall 311 and the upper plate 100 are opened by a predetermined interval H1 .

If the interval H1 between the first partition wall 311 and the upper plate 100 is 0.1 mm or more, the paths for each component moving up and down during centrifugation can maintain an appropriate interval, so that the Different components may be effectively separated by the first separating part 211 and the second separating part 212 . In addition, when the distance H1 between the first partition wall 311 and the upper plate 100 is 2 mm or less, the components separated by the first separator 211 and the second separator 212 after centrifugation are separated from each other. Mixing can be effectively prevented.

Next, the second partition wall 312 may be formed to have a predetermined distance H2 from the upper plate 100 . Specifically, the second partition wall 312 and the upper plate 100 may be formed to have a height spaced apart from each other by 0.1 mm to 2 mm, for example. Specifically, since the height of the second barrier rib 312 is slightly lower than the height of the main body 200 , it has a structure in which the second barrier rib 312 and the upper plate 100 are opened at a predetermined interval.

When the interval H2 between the second partition wall 312 and the upper plate 100 is 0.1 mm or more, the paths for each component moving up and down during centrifugation can maintain an appropriate interval, so that the Different components may be effectively separated by the second separation unit 212 and the third separation unit 213 . In addition, when the interval H2 between the second partition wall 312 and the upper plate 100 is 2 mm or less, the components separated by the second separation unit 212 and the third separation unit 213 after centrifugation are separated from each other. Mixing can be effectively prevented.

In this embodiment, the distance H1 between the first partition wall 311 and the upper plate 100 and the distance H2 between the second partition wall 312 and the upper plate 100 do not necessarily have to be the same. . This can be appropriately adjusted according to the characteristics of the component to be separated, for example, whether it is a solid component or a liquid component, and the amount of the component to be separated.

In addition, the first partition wall 311 and the second partition wall 312 may have a structure that protrudes from the lower plate 300 , for example, and protrudes from a sidewall inside the separation part 210 of the main body 200 . It may have a structure in the form in which it is applied, and the form is not particularly limited.

Next, the angle A1 between the bottom surface of the first separation part 211 region and the first partition wall 311 may be in the range of 91 degrees to 179 degrees, and more specifically, 95 degrees to 125 degrees or 97 degrees. degrees to 107 degrees. When the angle A1 between the lower plate of the first separation unit 211 region and the first partition wall 311 is less than 91 degrees, the cells in the sample positioned in the first separation unit 211 among the samples injected for centrifugation Since it is difficult for solid components, such as, to move beyond the first partition wall 311 to the second separation unit, there is a problem in that centrifugal separation efficiency is very low. In addition, when the angle A1 exceeds 179 degrees, the thickness of the first partition wall 311 becomes too thick, and accordingly, the amount of separation components that can be accommodated in the first separation part 211 is very small. . Therefore, when it is desired to separate more than a certain amount of components through centrifugation, a large area is required to increase the accommodation space of the first separation unit 211 , so the space efficiency is too low.

An angle A2 between the bottom surface of the second separation part 212 region and the first partition wall 311 may be in the range of 85 degrees to 179 degrees, and more specifically, 90 degrees to 160 degrees, or 90 degrees to 90 degrees. It may be in the range of 95 degrees. When the angle between the bottom surface of the second separation unit 212 region and the first partition wall 311 is less than 85 degrees, the liquid component in the sample positioned in the second separation unit 212 among the samples injected for centrifugation is It is difficult to move beyond the first partition wall 311 to the first separation unit. In addition, when the angle between the bottom surface of the second separation unit 212 region and the first partition wall 311 exceeds 179 degrees, it is difficult to secure sufficient space in the second separation unit 212 to accommodate the separated components. There is a problem with not enough space.

Next, the angle A3 between the bottom surface of the second separation part 212 region and the second partition wall 312 may be in the range of 91 degrees to 179 degrees, and more specifically, 95 degrees to 125 degrees, or 97 degrees. degrees to 107 degrees.

An angle A4 between the bottom surface of the third separation part 213 region and the second partition wall 312 may be in the range of 90 degrees to 179 degrees, and more specifically, 91 degrees to 160 degrees or 100 degrees to 110 degrees. It can also be a range.

When, for example, blood is introduced as a centrifugation sample, a buffy coat including a large number of white blood cells is located in the second separation unit 212 after centrifugation, and red blood cells are located in the third separation unit 213 . A component comprising a plurality of is located.

Accordingly, when blood is injected into the third separator for centrifugation to fill the second separator 212 and the first separator 211 and then centrifugation is performed, the blood components located in the third separator 213 are The component corresponding to the buffy coat moves to the area of the second separation unit 212 on the second partition wall 312 , and the component containing a large number of red blood cells among the components of the blood located in the second separation unit 212 is the second partition wall 312 . The second partition wall 312 moves to the third separation unit 213 .

At this time, when the angle A3 between the bottom surface of the second separation unit 212 region and the second partition wall 312 is less than 91 degrees, the third separation unit among the blood components located in the second separation unit 212 . There is a problem in that the component including a large number of red blood cells moving toward the 213 does not move smoothly due to friction with the second partition wall 312 . Therefore, it is preferable to configure the angle A3 to be 91 degrees or more.

In addition, when the angle A3 exceeds 179 degrees, it is separated after centrifugation to effectively form a space to contain a component located in the second separation unit 212, for example, a component corresponding to the buffy coat. is difficult

In addition, when the angle A4 between the bottom surface of the third separation unit 213 region and the second partition wall 312 is less than 90 degrees, the second separation of blood components located in the third separation unit 213 is There is a problem in that the movement of the component corresponding to the buffy coat moving toward the part 212 is not smooth. In addition, when the angle A4 is 179 degrees or more, it is difficult to secure a sufficient space in the third separation unit 213, so separation is not easy.

In this embodiment, the angles A3 and A4 between the second partition wall 312 and the bottom surface of the separation part may be greater than the angles A1 and A2 between the first partition wall 311 and the bottom surface of the separation part. . This is because both surfaces of the first partition wall 311 are the side where the liquid component mainly passes during centrifugal separation, so they are less affected by the frictional force, but the second partition wall 312 is the side through which the solid component such as cells passes, so the effect of the frictional force is large. because you receive

Therefore, in order to increase the centrifugal separation efficiency, the angles A3 and A4 between the second partition wall 312 and the bottom surface of the separation unit are greater than the angles A1 and A2 between the first partition wall 311 and the bottom surface of the separation unit. it is preferable

As described above, since the separation unit 210 includes the first separation unit 211 , the second separation unit 212 , and the third separation unit 213 , each component of the centrifuged sample is first separated for each component. The unit 211 , the second separation unit 212 , and the third separation unit 213 may be respectively located.

Referring back to FIGS. 1 and 2 , the main body 200 includes an inlet extension 271 , and the inlet extension 271 is connected to the inlet 341 positioned under the main body 200 . to be.

When a sample is injected into the inlet 101 of the upper plate 100 for centrifugal separation of a desired sample, the sample injected through the inlet extension 271 and the injection path 341 moves to the third separator 213 . do.

At this time, when the first vent hole 131 is opened, the sample injected into the third separation unit 213 by air circulation moves to the second separation unit 212 along the second partition wall 312 , and thereafter, the first The partition wall 311 may be moved toward the first separation unit 211 . In the present embodiment, the separation unit includes the first separation unit to the third separation unit, but if necessary, a separation unit may be further added.

The first vent hole 131 is preferably located in a region close to the rotation axis of the centrifugal separation device to be described later in the upper plate 100 . In addition, it is preferable that the sample to be injected for centrifugation, for example, blood, is filled from the region of the third separation unit located farthest from the axis of rotation of the centrifugal separation device. In this case, since the air pushed by the injected sample escapes through the upper first vent hole 131, sample injection is smooth. In addition, the sample can be quickly and easily injected into the chamber for a centrifugal separator without causing any bubbles in the sample and thus damaging the active ingredient, for example, cells, etc. included in the sample.

In the present embodiment, the upper surface of the separating unit 210 is an opening, but if necessary, the upper surface of the separating unit 210 may be in a closed form. In this way, when the separation unit has a sealed upper surface, a first vent hole extension (not shown) integral with the first vent hole may be formed on the upper surface of the first separation unit.

Meanwhile, the main body 200 includes a first valve unit 231 positioned to overlap a partial region of the first extraction path 321 . At this time, the first valve is positioned in the first valve unit 231 , and the first extraction path 321 is opened and closed by the first valve. Therefore, in order to extract the components separated by the first separation unit 211 after centrifugation, the first valve is operated to open the first extraction path 321 to first extract the components separated by the first separation unit It can be moved to the unit 221 .

The body 200 includes a third valve unit 233 positioned to overlap a partial region of the third extraction path 323 . At this time, a third valve is positioned in the third valve unit 233 , and the third extraction path 323 is opened and closed by the third valve. Therefore, in order to extract the components separated by the second separator 212 after centrifugation, the third valve is driven to open the third extraction passage 323 to remove the separated components in the second separator 212 . 3 It can be moved to the extraction unit 223 .

Meanwhile, although not shown, the components separated by the third separation unit 213 may also be withdrawn to the outside if desired. For example, the separated components of the third separation unit 213 may be extracted using a valve in a similar manner to withdrawing the components separated by the second separation unit 212 to the outside, and then may be withdrawn to the outside.

Referring back to FIG. 2 , the upper plate 100 includes an inlet 101 for injecting a sample for centrifugation, and a first outlet 121 and a second outlet for extracting each centrifuged component to the outside at the same time. Two outlets 122 are included.

The first outlet 121 and the second outlet 122 are the same as those described above, and detailed descriptions thereof will be omitted herein.

Meanwhile, although not shown, the inlet 101 may also include an inlet stopper. The inlet stopper is made of, for example, a material having elasticity, and may have a ring shape surrounding the rim of the inlet. As such, the inclusion of the inlet stopper prevents the injected sample from popping out due to unintended circumstances when the centrifuge chamber is operated, such as rotation, when injecting a sample for centrifugation, and mixing of contaminants in the outside air It functions to prevent over-contamination.

The upper plate 100 includes a first vent hole 131 and a second vent hole 132 together with the injection hole 101 , the first outlet 121 , and the second outlet 122 .

The first vent hole 131 is located on the upper surface of the first separation unit 211 located in the main body 200 . If necessary, when the upper surface of the separation unit 210 has a closed structure, a first vent hole extension (not shown) may be formed on the upper surface of the first separation unit 211 .

The second vent hole 132 is connected to the second vent hole extension part 252 included in the first extraction part 221 located in the main body 200 .

In addition, the upper plate 100 includes a first valve hole 111 , a second valve hole 112 , and a third valve hole 113 .

The first valve hole 111 is connected to the first valve unit 231 .

The first valve unit 231 is positioned to overlap a partial region of the first extraction path 321 positioned in the main body 200 . The first valve located in the first valve unit 231 is driven through the first valve hole 111, and is separated from the first separation unit by opening and closing the first extraction path 321 through the first valve. You can control the movement of ingredients.

The second valve hole 112 is connected to the second valve unit 232 .

The second valve unit 232 is positioned to overlap a partial region of the second extraction path 322 positioned in the main body 200 . The second valve located in the second valve unit 232 is driven through the second valve hole, and the component accommodated in the first extraction unit 221 by opening and closing the second extraction path 322 through the second valve can control the movement of

The third valve hole 113 is connected to the third valve unit 233 .

The third valve unit 233 is positioned to overlap a partial region of the third extraction path 323 . The third valve located in the third valve unit 233 is driven through the third valve hole 113, and by opening and closing the third extraction path 323 through the third valve, the second separation unit 212 is It is possible to control the movement of separated components into the

The lower plate 300 seals the opening partially formed in the lower portion of the main body 200 .

In this embodiment, the structure in which the first partition wall 311 and the second partition wall 312 are formed in the main body 200 is illustrated. However, the first partition wall 311 and the second partition wall 312 may be formed to protrude from the lower plate 300 if necessary.

6 is a plan view schematically showing a chamber for a centrifugal separation device according to another embodiment, and FIG. 7 is an exploded perspective view of the chamber for a centrifugal separation device of FIG. 6 .

6 and 7 , the chamber 500 for a centrifugal separation device according to another embodiment includes an upper plate 100 , a body 200 , and a lower plate 300 .

In the chamber for the centrifugal separation device of the present embodiment, the upper plate 100 includes a first inlet 401 and a second inlet 402 .

Also, the body 200 includes a first inlet extension 411 and a second inlet extension 412 .

The first inlet 401 is for injecting a sample, for example, blood for centrifugation, and the second inlet 402 is a specific component of the sample, for example, one of the blood components when blood is injected into the sample. This is to inject a Ficoll solution, etc. for isolating only peripheral blood mononuclear cells (PBMC).

Conventionally, in order to isolate only these PBMCs, a Ficoll solution was put into one test tube, blood was carefully injected thereon, and two layers were formed, followed by centrifugation. However, as described above, when the chamber for a centrifugal separation device including the first and second inlets 401 and 402 is used, a desired component can be obtained by conveniently and easily injecting the sample and the Ficoll solution.

In this embodiment, the case including two injection holes has been illustrated and described, but if necessary, the injection holes may be further added.

The sample injected through the first inlet 401 passes through the first inlet extension 411 and is then separated through the first injection path 421 connected to the second separator 212 . It is moved to the second and first separations.

The Ficoll solution injected through the second inlet 402 passes through the second inlet extension 412 and then moves to the third separation unit through the second injection path 422 connected to the third separation unit. do. As such, when a sample, for example, blood and a Ficoll solution are injected using the two inlets, the Ficoll solution is positioned outside the blood with respect to the chamber for the centrifugal separation device. When centrifugation is performed, the Ficoll solution moves to the second separation unit due to the specific gravity difference due to centrifugal force to form a PBMC layer.

6 and 7, the first injection hole 401, the second injection hole 402, the first injection hole extension part 411, the second injection hole extension part 412, the first injection path 421 and the second injection hole Configurations other than the furnace 422 are the same as those of the chamber for a centrifugal separation device according to an embodiment described with reference to FIGS. 1 and 2 , and have the same characteristics as those described above. Accordingly, in FIGS. 6 and 7, the same reference numerals as in FIGS. 1 and 2 are used.

8A is a cross-sectional view of the centrifugal separation device chamber of FIG. 6 taken along line A-A', and FIGS. 8B and 8C are plan and cross-sectional views of the first outlet stopper, respectively.

Referring to FIG. 8A , the lower end of the first outlet 121 includes a first outlet stopper 121a for sealing the inner circumferential lower surface of the first outlet 121 and sealing the upper surface of the first outlet 241 at the same time. and the first outlet stopper 121a includes a first cutout 121aa.

A detailed description of the first outlet 121, the first outlet 241, the first outlet stopper 121a, and the first cutout 121aa shown in FIGS. 8A to 8C is a centrifugal separation device according to an embodiment. It is the same as described with reference to FIGS. 3A to 3C in the chamber for use. Accordingly, the same reference numerals as in FIGS. 3A to 3C are used in FIGS. 8A to 8C.

9A is a cross-sectional view of the centrifugal separation device chamber of FIG. 6 taken along line B-B', and FIGS. 9B and 9C are plan views and cross-sectional views of the second outlet stopper, respectively.

Referring to FIG. 9A , the lower end of the second outlet 122 includes a second outlet stopper 122a for sealing the inner circumferential lower surface of the second outlet 122 and sealing the upper surface of the second outlet 242 at the same time. and the second outlet stopper 122a includes a second cutout 122aa.

A detailed description of the second outlet 122, the second outlet 242, the second outlet stopper 122a, and the second cutout 122aa shown in FIGS. 9A to 9C is a centrifugal separation device according to an embodiment. It is the same as described with reference to FIGS. 4A to 4C in the chamber for use. Accordingly, the same reference numerals as in FIGS. 4A to 4C are used in FIGS. 9A to 9C.

10 is a cross-sectional view taken along line I-II of the chamber for the centrifugal separation device of FIG. 6 .

Referring to FIG. 6 , the separation unit 210 includes a first separation unit 211 , a second separation unit 212 , and a third separation unit 213 positioned side by side in one direction. In this case, each region of the first separation unit 211 and the second separation unit 212 is separated by a first partition wall 311 located in the separation unit 210 .

In addition, the first partition wall 311 may be formed to have a predetermined distance H1 from the upper plate 100 , and may be formed to have predetermined angles A1 and A2 from the bottom surface.

The second partition wall 312 may be formed to have a predetermined distance H2 from the upper plate 100 , and may be formed to have predetermined angles A3 and A4 with respect to the bottom surface.

The characteristics of the first and second partition walls as described above are the same as those described with reference to FIG. 5 in the chamber for a centrifugal separation device according to an embodiment. Therefore, in FIG. 10, the same reference numerals as in FIG. 5 are used.

In the chamber for a centrifugal separation device according to this embodiment, the remaining components other than the above-described configuration are the same as those described in the chamber for a centrifugal separation device of an embodiment described with reference to FIGS. 1 to 5, and detailed descriptions thereof will be omitted. do.

According to the chamber for a centrifugal separation device according to the present embodiment, it is possible to easily separate the centrifuged components after injecting the sample by component without a separate manual operation, and mixing between the separated components even when the rotational motion is stopped after centrifugation can prevent Therefore, each separated component can be easily obtained with high quality.

In addition, since only a desired component can be selectively and easily extracted from each component separated by using a valve, user convenience can be remarkably increased.

In addition, according to the embodiment described with reference to FIG. 4, since it includes a plurality of inlets, when it is necessary to input other materials necessary for centrifugation in addition to a sample, for example, blood, it is possible to extract a desired component by simply injecting it.

11 is a plan view schematically illustrating a centrifugal separation device according to an embodiment.

Referring to FIG. 11 , the centrifugal separation apparatus 1000 according to the embodiment may include at least two chambers 500 for the centrifugal separation apparatus of the above-described embodiments.

At this time, the two or more chambers 500 for the centrifugal separation device are positioned to be symmetrical to each other about the rotation axis 600 in the centrifugal separation device 1000 . 11 shows only a case in which two chambers 500 for centrifugal separation device are included for convenience, but if symmetrically arranged as described above, the number of chambers 500 for centrifugal separation device can be appropriately increased if necessary.

According to the chamber for a centrifugal separation device according to the present embodiment, it is possible to easily separate the centrifuged components after injecting the sample by component without a separate manual operation, and mixing between the separated components even when the rotational motion is stopped after centrifugation can prevent Therefore, each separated component can be easily obtained with high quality.

In addition, since only a desired component can be selectively and easily extracted from each component separated by using a valve, user convenience can be remarkably increased.

In particular, since the extraction unit in the chamber for the centrifugal separation device of the present embodiments includes an extract receiving unit and a contaminant receiving unit separated by a contamination prevention wall, re-contamination in the process of extracting the centrifuged components after centrifuging the biological fluid It has the advantage that it can be easily separated without

In addition, according to the embodiment described with reference to FIG. 6, since it includes a plurality of inlets, when it is necessary to input other materials necessary for centrifugation in addition to a sample, for example, blood, a desired component can be extracted by simply putting it in.

9 is a plan view schematically illustrating a centrifugal separation device according to an embodiment.

Referring to FIG. 9 , the centrifugal separation apparatus 1000 according to an embodiment may include at least two or more chambers 500 for the centrifugal separation apparatus of the above-described embodiments.

At this time, the two or more chambers 500 for the centrifugal separation device are positioned to be symmetrical to each other about the rotation axis 600 in the centrifugal separation device 1000 . In FIG. 9 , only the case including two chambers 500 for centrifugal separation device is illustrated for convenience, but if symmetrically arranged as described above, the number of chambers 500 for centrifugal separation device can be appropriately increased if necessary.

The present invention is not limited to the above embodiments, but can be manufactured in various different forms, and those of ordinary skill in the art to which the present invention pertains can take other specific forms without changing the technical spirit or essential features of the present invention. It will be understood that it can be implemented as Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

1000: centrifugation device
500: chamber for centrifugation device
100: top plate
200: body
300: lower plate
121: first outlet
121a: first stopper
122: second outlet
122a: first stopper
210: separation unit
211: first separation unit
212: second separation unit
213: third separation unit
220: extraction unit
221: first extraction unit
222: second extraction unit
223: third extraction unit
241: first withdrawal unit
242: second withdrawal unit
311: first bulkhead
312: second bulkhead
101: inlet

Claims (25)

It includes a main body, an upper plate located on the upper part of the main body, and a lower plate for sealing the opening of the lower part of the main body,
The body may include first to third separation units in which each component of the centrifuged sample is separated, and a first extraction unit to move and receive components located in the first separation unit; a second extraction unit for further separating the components accommodated in the first extraction unit and then moving and accommodating; and a third extraction unit for moving and accommodating components located in the second separation unit,
The upper plate may include an inlet for injecting a sample for centrifugation; and a first outlet and a second outlet for withdrawing the separated components accommodated in the second and third extracting units to the outside, respectively. According to claim 1,
A chamber for a centrifugal separation device comprising a first outlet stopper sealing the inner circumferential lower surface of the first outlet at a lower end of the first outlet. 3. The method of claim 2,
The second extraction unit includes a first extraction unit,
The first outlet stopper includes a first cutout formed so that a withdrawal tool for withdrawing the additional centrifuged component contained in the second extraction unit can be inserted into the first withdrawal unit through the first outlet. Chamber for centrifugal separators. 3. The method of claim 2,
An average thickness of the first outlet stopper is in the range of 0.1 mm to 10 mm for a centrifugal separation device chamber. 4. The method of claim 3,
The first cut-out portion has at least one shape of a straight line shape, a cross shape, and an asterisk shape. 4. The method of claim 3,
The first cut-out chamber for a centrifugal separation device that is impermeable to liquid, gas to pass through. 4. The method of claim 3,
The average interval of the first incision is in the range of 0.01mm to 2mm for a centrifugal separation device chamber. 4. The method of claim 3,
An average length of the first incision is in the range of 0.01 mm to 10 mm for a centrifugal separation device chamber. 4. The method of claim 3,
A chamber for a centrifugal separation device, wherein a portion of the wall of the first lead-out portion has an inclination in the thickness direction. According to claim 1,
and a second outlet stopper at a lower end of the second outlet to seal an inner circumferential lower surface of the second outlet. 11. The method of claim 10,
The third extraction unit includes a second extraction unit,
The second outlet stopper includes a second cutout formed so that a withdrawal tool for withdrawing the centrifuged component contained in the third extraction unit can be inserted into the second withdrawal unit through the second outlet. Chamber for separation device. 12. The method of claim 11,
A chamber for a centrifugal separation device, wherein a portion of the wall of the second lead-out portion has an inclination in the thickness direction. It includes a main body, an upper plate located on the upper part of the main body, and a lower plate for sealing the opening of the lower part of the main body,
The body may include first to third separation units in which each component of the centrifuged sample is separated; a first extraction unit for moving and accommodating components located in the first separation unit; a second extraction unit for further separating the components accommodated in the first extraction unit and then moving and accommodating; and a third extraction unit for moving and accommodating each component located in the second separation unit,
The upper plate may include a first inlet for injecting a sample for centrifugation; a second inlet for injecting the Ficoll solution; and a first outlet and a second outlet for withdrawing the separated components accommodated in the second and third extracting units to the outside, respectively. 14. The method of claim 13,
A chamber for a centrifugal separation device comprising a first stopper sealing the inner peripheral lower surface of the first outlet at a lower end of the first outlet. 15. The method of claim 14,
The second extraction unit includes a first extraction unit,
The first outlet stopper includes a first cutout formed so that a withdrawal tool for withdrawing the additional centrifuged component contained in the second extraction unit can be inserted into the first withdrawal unit through the first outlet. Chamber for centrifugal separators. 15. The method of claim 14,
An average thickness of the first outlet stopper is in the range of 0.1 mm to 10 mm for a centrifugal separation device chamber. 16. The method of claim 15,
The first cut-out portion has at least one shape of a straight line shape, a cross shape, and an asterisk shape. 16. The method of claim 15,
The first cut-out chamber for a centrifugal separation device that is impermeable to liquid, gas to pass through. 16. The method of claim 15,
The average interval of the first incision is in the range of 0.01mm to 2mm for a centrifugal separation device chamber. 16. The method of claim 15,
An average length of the first incision is in the range of 0.01 mm to 10 mm for a centrifugal separation device chamber. 16. The method of claim 15,
A chamber for a centrifugal separation device, wherein a portion of the wall of the first lead-out portion has an inclination in the thickness direction. 14. The method of claim 13,
and a second outlet stopper at a lower end of the second outlet to seal an inner circumferential lower surface of the second outlet. 23. The method of claim 22,
The third extraction unit includes a second extraction unit,
The second outlet stopper includes a second cutout formed so that a withdrawal tool for withdrawing the centrifuged component contained in the third extraction unit can be inserted into the second withdrawal unit through the second outlet. Chamber for separation device. 24. The method of claim 23,
A chamber for a centrifugal separation device, wherein a portion of the wall of the second lead-out portion has an inclination in the thickness direction. A centrifugal separation device comprising at least two chambers for the centrifugal separation device according to claim 1 .

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