CN215465213U - Cell centrifugal device - Google Patents
Cell centrifugal device Download PDFInfo
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- CN215465213U CN215465213U CN202121524433.9U CN202121524433U CN215465213U CN 215465213 U CN215465213 U CN 215465213U CN 202121524433 U CN202121524433 U CN 202121524433U CN 215465213 U CN215465213 U CN 215465213U
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Abstract
The utility model provides a cell centrifugation device, which comprises a centrifugation cup and a liquid pushing plate; the centrifugal cup is provided with a rotating axis, is connected with the rotating driving mechanism and is driven by the rotating driving mechanism to rotate around the rotating axis so as to be used for centrifuging blood samples contained in the centrifugal cup; the liquid pushing plate is arranged in the centrifugal cup, and is used for pushing the blood sample to synchronously rotate along the same rotation direction with the centrifugal cup under the condition of autorotation of the centrifugal cup. According to the utility model, the liquid pushing plate is arranged in the centrifugal cup, and in the process of centrifuging the cup, a blood sample in the centrifugal cup synchronously rotates with the centrifugal cup under the pushing action of the liquid pushing plate, so that cells in the blood sample are prevented from being sheared in the centrifuging process, and the shearing damage to the cells in the centrifuging process is reduced.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to a cell centrifugation device.
Background
In the field of cell medicine, it is often necessary to culture cells collected from a living body and then inoculate the cells to a patient, and in order to ensure the therapeutic effect of the cells collected and cultured from the living body, it is necessary to perform peripheral blood mononuclear cell sorting (PBMC sorting) on the cells, and at the same time, it is necessary to remove impurities and culture medium during the cell culture process and to concentrate the cells to a cell concentration suitable for therapy. The method for sorting the peripheral blood mononuclear cells is a density gradient centrifugation method, namely, different particles in a blood sample are settled at a certain speed under the action of centrifugal force, and zones are finally formed on different areas so as to achieve the purpose of sorting.
The inner side wall of the existing centrifugal equipment drives outer liquid close to the inner side wall to rotate by virtue of viscous resistance in the centrifugal process, the outer liquid drives inner liquid far away from the inner side wall to rotate by virtue of the viscous resistance, and finally, the liquid in the centrifugal equipment is in a rotating state.
SUMMERY OF THE UTILITY MODEL
The utility model provides a cell centrifugal device, which is used for solving the problem that the existing centrifugal equipment is easy to cause shearing damage to cells in the centrifugal process.
The present invention provides a cell centrifuge device comprising: a centrifuge cup and a liquid pushing plate; the centrifugal cup is provided with a rotating axis, is connected with the rotating driving mechanism and is driven by the rotating driving mechanism to rotate around the rotating axis so as to be used for centrifuging a blood sample contained in the centrifugal cup; one or more liquid pushing plates are arranged in the centrifugal cup; under the condition of the autorotation of the centrifugal cup, the liquid pushing plate is used for pushing the blood sample to synchronously rotate along the same rotation direction as the centrifugal cup.
According to the cell centrifugal device provided by the utility model, one end of the liquid pushing plate is close to the rotating axis, and the other end of the liquid pushing plate extends towards the inner side wall of the centrifugal cup; the projection of the first side edge of the liquid pushing plate on the plane where the bottom of the centrifugal cup is located is linear or arc-shaped, and the second side edge of the liquid pushing plate is perpendicular to the bottom of the centrifugal cup; and under the condition that the projection of the first side edge of the liquid pushing plate on the plane of the bottom of the centrifuge cup is a straight line, the first side edge of the liquid pushing plate extends along the radial direction of the centrifuge cup.
According to the cell centrifugation device provided by the utility model, a first gap is formed between one end, close to the inner side wall of the centrifugation cup, of the liquid pushing plate and the inner side wall; one end of the liquid pushing plate close to the cup bottom of the centrifugal cup is attached to the cup bottom.
According to the cell centrifuge device provided by the utility model, under the condition that the number of the liquid pushing plates is multiple, the liquid pushing plates are uniformly distributed in a circumference direction relative to the rotation axis.
According to the present invention, there is provided a cell centrifuge, further comprising: a cup cover; the cup cover is matched with the cup opening of the centrifugal cup; a first liquid passing structure is arranged on the cup cover, and a second liquid passing structure is arranged on the centrifugal cup; the first end of the first liquid passing structure is arranged on the outer side surface of the cup cover and distributed along the rotation axis, and the second end of the first liquid passing structure is arranged on the inner side surface of the cup cover; the second liquid passing structure comprises a liquid passing pipe and a liquid passing channel; the liquid passing pipe is arranged in the centrifugal cup, the first end of the liquid passing pipe is communicated with the second end of the first liquid passing structure, and the second end of the liquid passing pipe is arranged at the bottom of the centrifugal cup; the liquid passing channel is arranged in the shell wall of the cup bottom, the first end of the liquid passing channel is communicated with the second end of the liquid passing pipe, and the second end of the liquid passing channel is arranged on the inner side surface of the cup bottom and is communicated with the inner cavity of the centrifugal cup.
According to the cell centrifugal device provided by the utility model, a third liquid passing structure is arranged in the shell wall of the cup bottom of the centrifugal cup; the first end of the third liquid passing structure is arranged on the outer side surface of the cup bottom and distributed along the rotation axis, and the second end of the third liquid passing structure is arranged on the inner side surface of the cup bottom and communicated with the inner cavity of the centrifugal cup.
According to the cell centrifugal device provided by the utility model, the first liquid passing structure comprises a connecting pipe; the connecting pipe and the liquid through pipe are distributed along the rotating axis; a liquid passage and a gas passage are arranged in the connecting pipe, a liquid interface is arranged at the first end of the liquid passage, and the second end of the liquid passage is communicated with the first end of the liquid through pipe; the first end of the gas passage is provided with a gas interface, and the second end of the gas passage is communicated with the inner cavity of the centrifugal cup.
According to the cell centrifugal device provided by the utility model, the cup cover is provided with the air holes; the air holes are at least one, and air permeable films are arranged in the air holes.
The cell centrifugal device provided by the utility model has the advantages that the liquid pushing plate is arranged in the centrifugal cup, under the condition that the centrifugal cup rotates automatically, a blood sample in the centrifugal cup rotates synchronously with the centrifugal cup under the pushing action of the liquid pushing plate, namely, in the rotating direction of the centrifugal cup, relative motion does not exist between outer layer cells close to the inner side wall of the centrifugal cup and inner layer cells close to the rotating axis, and the rotating power source of the inner layer cells is not viscous resistance between the outer layer cells and the inner layer cells but thrust of the liquid pushing plate, so that the shearing action on the cells when the viscous resistance acts on the cells is avoided, and the shearing damage to the cells in the centrifugal process is reduced.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, some brief descriptions will be given below to the drawings used in the description of the embodiments or the prior art, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic view of the structure of a cell centrifuge apparatus according to the present invention;
FIG. 2 is a schematic diagram of the force applied to the cells during centrifugation;
FIG. 3 is a schematic diagram of the force of centrifugation of cells provided by the present invention when a push plate is provided;
FIG. 4 is a schematic view of a first arrangement of the pusher plate in the centrifuge cup provided by the present invention;
FIG. 5 is a schematic view of a second arrangement of the pusher plate in the centrifuge cup provided by the present invention;
FIG. 6 is a schematic top view of the cell centrifuge apparatus according to the present invention;
FIG. 7 is a schematic cross-sectional view taken along the line A-A of FIG. 6 according to the present invention;
FIG. 8 is a schematic cross-sectional view taken along the line A-A of FIG. 6 according to the present invention;
FIG. 9 is a schematic view of a lid according to the present invention;
FIG. 10 is a second schematic view of the lid of the present invention;
FIG. 11 is a broken line schematic diagram of the number of cells in the cell culture process provided by the present invention;
reference numerals:
1: a centrifuge cup; 11: a second liquid-passing structure; 111: a liquid pipe is communicated;
112: a liquid passage; 2: a liquid pushing plate; 21: a pin shaft;
3: a cup cover; 31: a first liquid passing structure; 311: a connecting pipe;
3111: a liquid passage; 3112: a gas passage; 3113: a liquid interface;
3114: a gas interface; 32: air holes are formed; 33: a top cover;
34: an edge; 4: a rotation driving mechanism; 5: and (6) observing holes.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A cell centrifuge device according to the present invention will be described with reference to FIGS. 1 to 11.
As shown in fig. 1 to 10, the present embodiment provides a cell centrifuge, including: the centrifugal cup 1 is connected with the rotary driving mechanism 4, and is driven by the rotary driving mechanism 4 to rotate around the rotary axis so as to be used for centrifuging a blood sample contained in the centrifugal cup 1; the number of the liquid pushing plates 2 is one or more, the liquid pushing plates are arranged in the centrifugal cup 1, and under the condition that the centrifugal cup 1 rotates, the liquid pushing plates 2 are used for pushing the blood sample to rotate synchronously along the same rotating direction as the centrifugal cup 1.
Specifically, in the present embodiment, the liquid pushing plate 2 is disposed in the centrifuge cup 1, and under the condition that the centrifuge cup 1 rotates, a blood sample in the centrifuge cup 1 rotates synchronously with the centrifuge cup 1 under the pushing action of the liquid pushing plate 2, that is, in the rotation direction of the centrifuge cup 1, there is no relative motion between the outer layer cell close to the inner side wall of the centrifuge cup 1 and the inner layer cell close to the rotation axis, and the power source for the rotation of the inner layer cell is no longer the viscous resistance between the outer layer cell and the inner layer cell, but is the pushing force of the liquid pushing plate, so as to avoid the shearing action on the cell when the viscous resistance acts on the cell, and reduce the shearing damage to the cell in the centrifugation process.
It should be noted that the blood sample shown in this embodiment contains target cells to be extracted, and the blood sample is centrifuged by the centrifuge device, so that the target cells and other particles in the blood sample are layered to extract the target cells.
As shown in FIG. 2, the centrifugal cup 1 of this embodiment performs a centrifugal operation on a blood sample when the liquid pushing plate 2 is not disposed, the dotted line is a movement track of cells in the blood sample, and outer cells near the inner sidewall of the centrifugal cup 1 are supported by viscous resistance between liquids, so as to drive inner cells near the rotation axis of the centrifugal cup 1 to rotate, in the process, the rotation of the inner layer cells has a hysteresis phenomenon, namely, in the rotating direction of the centrifugal cup 1, the relative motion exists between the outer layer cells and the inner layer cells, under the condition of relative movement, the outer layer cell and the inner layer cell are both subjected to a shearing force tau, the direction of the shearing force tau is along the tangential direction of the movement track, the shearing force tau applied to the outer layer cell is larger, the shearing force tau applied to the inner layer cell is smaller, under the condition that the shearing force tau acts on the cells, the shearing force tau can cause shearing damage to the cells; in addition, for one of the cells, the blood sample flow rate is higher on the side close to the inner side wall and lower on the side close to the rotation axis, so that the cell is also subjected to a radial pressure, which also causes shear damage to the cell when the pressure is applied to the cell.
As shown in fig. 3, the centrifuge cup 1 shown in this embodiment performs a centrifugation operation on a blood sample when the push plate 2 is disposed, the dotted line is a movement track of cells in the blood sample, outer-layer cells and outer-layer cells synchronously rotate under the action of the pushing force F of the push plate, and a power source for the rotation of inner-layer cells is no longer viscous resistance between the outer-layer cells and the inner-layer cells, so that there is no hysteresis in the rotation of the inner-layer cells, that is, there is no relative movement between the outer-layer cells and the inner-layer cells in the rotation direction of the centrifuge cup 1, and the outer-layer cells and the inner-layer cells are not subjected to a shearing force without the relative movement, that is, there is no shearing damage to the cells during the centrifugation process.
It should be noted that the number of the liquid pushing plate 2 may be 1, 2, 3, or 4, and the like, and is not limited thereto.
Preferably, as shown in fig. 4 and 5, the liquid pushing plate 2 shown in the present embodiment has one end close to the rotation axis and the other end extending toward the inner side wall of the centrifugal cup 1; the projection of the first side edge of the liquid pushing plate 2 on the plane where the bottom of the centrifugal cup 1 is located is linear or arc-shaped, and the second side edge of the liquid pushing plate 2 is perpendicular to the bottom of the centrifugal cup 1.
Specifically, when the projection of the first side edge of the liquid pushing plate 2 on the plane where the bottom of the centrifuge cup 1 is located is a straight line, the plate surface of the liquid pushing plate 2 for pushing the blood sample to rotate is a plane; when the projection of the first side edge of the liquid pushing plate 2 on the plane where the bottom of the centrifugal cup 1 is located is arc-shaped, the plate surface of the liquid pushing plate 2 used for pushing blood samples to rotate is an arc surface, and the liquid pushing plate in a corresponding shape is selected according to different blood sample concentrations so as to ensure that the blood samples achieve a good centrifugal effect.
Preferably, the liquid pushing plate 2 shown in the embodiment is fixedly connected with the bottom of the centrifuge cup 1, or the liquid pushing plate 2 is detachably connected with the bottom of the centrifuge cup 1.
Specifically, the liquid pushing plate 2 is detachably connected with the bottom of the centrifugal cup 1, so that the centrifugal cup 1 is convenient to clean and the liquid pushing plate 2 is convenient to replace.
Wherein, the fixed connection shown in the embodiment comprises bonding; the detachable connection comprises a pin shaft connection or a bolt connection, or a clamping structure is arranged at the bottom of the centrifugal cup 1, and the liquid pushing plate is clamped in the clamping structure.
In one embodiment, as shown in fig. 1, 4, 5, 7 and 8, the liquid pushing plate 2 is connected with the cup bottom through a pin shaft 21, the liquid pushing plate 2 is provided with a pin shaft hole along the rotation axis direction, the cup bottom is provided with a fixing hole, one end of the pin shaft 21 is connected with the pin shaft hole, and the other end of the pin shaft 21 is connected with the fixing hole, so as to realize the detachable connection of the liquid pushing plate 2 and the cup bottom; in order to ensure the connection stability, two pin shaft holes are formed, and the fixing holes are correspondingly arranged with the pin shaft holes one to one.
In another embodiment, a first clamping groove is formed in the cup bottom along the radial direction of the centrifugal cup 1, the liquid pushing plate 2 is clamped in the first clamping groove, and the liquid pushing plate 2 and the first clamping groove are in interference fit; or a second clamping groove is formed in the inner side wall of the centrifugal cup 1 along the rotation axis direction, the liquid pushing plate 2 is clamped in the second clamping groove, and the liquid pushing plate 2 and the second clamping groove are in interference fit; or the inner side walls of the cup bottom and the centrifugal cup 1 are respectively provided with a first clamping groove and a second clamping groove, the first clamping groove and the second clamping groove are arranged in a one-to-one correspondence manner, the liquid pushing plate 2 is clamped into the first clamping groove and the second clamping groove simultaneously, and the liquid pushing plate 2 is in interference fit with the first clamping groove and the second clamping groove.
Preferably, as shown in fig. 4, 7 and 8, a first gap is formed between one end of the liquid pushing plate 2 close to the inner side wall of the centrifuge cup 1 and the inner side wall; one end of the liquid pushing plate 2 close to the cup bottom of the centrifugal cup 1 is attached to the cup bottom.
Specifically, in the centrifugation process of the centrifugal cup 1, the target cells in the blood sample gradually approach the inner side wall of the centrifugal cup 1 and are finally attached to the inner side wall of the centrifugal cup 1, and the target cells can pass through the first gap, so that the phenomenon of concentrated accumulation of the target cells is avoided; meanwhile, blood samples can freely pass through the first gap, and the liquid level heights of the two sides of the liquid pushing plate 2 are kept consistent.
It should be noted here that the first gap is not set too large, the liquid pushing plate 2 cannot achieve a good liquid pushing effect when the first gap is set too large, the ratio of the first gap to the inner radius of the centrifugal cup 1 is in the range of 1/600-1/20, and the ratio of the first gap to the inner radius of the centrifugal cup 1 may be 1/600, 1/300, 1/100, 1/60, 1/40 or 1/20, wherein 1/60 is preferred.
Preferably, as shown in fig. 1, 4 and 5, a second gap is provided between one end of the liquid pushing plate 2 close to the rotation axis and the rotation axis.
Specifically, a certain operation space is provided for the installation and the disassembly of the liquid pushing plate 2 by arranging a second gap between the liquid pushing plate 2 and the rotating axis, meanwhile, the linear velocity of inner-layer cells close to the rotating axis is smaller in the centrifugal process, and the arrangement of the second gap has smaller influence on the centrifugal effect of the blood sample.
It should be noted here that the ratio of the second gap to the inner radius of the centrifuge cup 1 is in the range of 1/200-1/5, and the ratio of the second gap to the inner radius of the centrifuge cup 1 may be 1/200, 1/100, 1/60, 1/30, 1/10 or 1/5, wherein 1/30 is preferred.
Preferably, in the case where the liquid pushing plate 2 is plural, the plural liquid pushing plates 2 are circumferentially evenly distributed with respect to the rotation axis.
Specifically, the plurality of liquid pushing plates 2 divide the centrifugal cup 1 into a plurality of relatively independent centrifugal areas, and the blood samples in each centrifugal area are ensured to have the same centrifugal effect by uniformly distributing the liquid pushing plates 2 along the circumferential direction of the rotation axis.
In one embodiment, two liquid pushing plates 2 are provided, and the included angle between the two liquid pushing plates 2 is 180 degrees.
In another embodiment, three liquid pushing plates 2 are provided, and the included angle between two adjacent liquid pushing plates 2 is 120 degrees.
In another embodiment, as shown in fig. 1 and 4, the number of the liquid pushing plates 2 is four, and the included angle between two adjacent liquid pushing plates 2 is 90 °.
Preferably, as shown in fig. 6, 7 and 8, the centrifugal device of the present embodiment further includes a lid 3; the cup cover 3 is matched with the cup opening of the centrifugal cup 1; the cup cover 3 is provided with a first liquid passing structure 31; the first end of the first liquid passing structure 31 is arranged on the outer side surface of the cup cover 3 and distributed along the rotation axis, and the second end of the first liquid passing structure 31 is arranged on the inner side surface of the cup cover 3.
Specifically, through set up first logical liquid structure 31 on bowl cover 3, can realize adding the blood sample in the centrifuge cup 1 under the condition of not opening bowl cover 3.
Preferably, as shown in fig. 1, 4, 5, 7 and 8, the centrifuge cup 1 shown in this embodiment is provided with a second liquid passing structure 11; the second liquid passing structure 11 comprises a liquid passing pipe 111 and a liquid passing channel 112; the liquid flowing pipe 111 is arranged in the centrifugal cup 1, the first end of the liquid flowing pipe 111 is communicated with the second end of the first liquid flowing structure 31, the second end of the liquid flowing pipe 111 is arranged at the bottom of the centrifugal cup 1, the liquid flowing channel 112 is arranged in the shell wall of the bottom of the cup, the first end of the liquid flowing channel 112 is communicated with the second end of the liquid flowing pipe 111, and the second end of the liquid flowing channel 112 is arranged on the inner side surface of the bottom of the cup and is communicated with the inner cavity of the centrifugal cup 1.
Specifically, in order to prevent the blood sample from splashing when the blood sample is added into the centrifuge cup through the first liquid passing structure 31, the second liquid passing structure 11 is arranged, so that the blood sample enters the liquid passing tube 111 after passing through the first liquid passing structure 31, then enters the liquid passing channel 112, and finally enters the inner cavity of the centrifuge cup 1 from the cup bottom, and the stability of the blood sample in the injection process is ensured; meanwhile, the liquid passage 112 is embedded in the wall of the cup bottom, so that the liquid passage 112 is prevented from occupying the effective space of the inner cavity of the centrifugal cup; furthermore, the blood sample after centrifugation yields a cell suspension which can be withdrawn through the second and first liquid passing structures 11 and 31.
In one embodiment, as shown in fig. 7 and 8, the liquid feed tube 111 is preferably arranged along the rotation axis to improve the stability of the centrifuge cup 1 during rotation.
Preferably, as shown in fig. 7 and 8, one end of the liquid pushing plate 2 close to the cup cover 3 is attached to the cup cover 3.
Specifically, through setting up the one end that the push pedal 2 is close to bowl cover 3 and laminating mutually with bowl cover 3, can prevent effectively that the blood sample that is located push pedal 2 one side from getting into the opposite side of push pedal 2 through the one end that push pedal 2 is close to bowl cover 3 at centrifugal process.
Preferably, a third liquid passing structure is arranged in the shell wall of the centrifugal cup 1 shown in the embodiment; the first end of the third liquid passing structure is arranged on the outer side surface of the cup bottom and distributed along the rotation axis, and the second end of the third liquid passing structure is arranged on the inner side surface of the cup bottom and communicated with the inner cavity of the centrifugal cup 1.
Specifically, a blood sample can be injected into the inner cavity of the centrifuge cup 1 from the bottom of the cup through the third liquid passing structure, and accordingly, a cell suspension can be extracted through the third liquid passing structure.
Preferably, as shown in fig. 7, 8 and 9, the first liquid passing structure 31 shown in the present embodiment includes a connection pipe 311; the connecting tube 311 and the liquid flowing tube 111 are distributed along the rotation axis, a liquid passage 3111 and a gas passage 3112 are arranged in the connecting tube 311, a liquid port 3113 is arranged at a first end of the liquid passage 3111, and a second end of the liquid passage 3111 is communicated with a first end of the liquid flowing tube 111; a first end of the gas passage 3112 is provided with a gas port 3114, and a second end of the gas passage 3112 is communicated with the inner cavity of the centrifugal cup 1.
Specifically, in the centrifuge cup 1 shown in this embodiment, when used for cell culture, a culture medium can be injected into the centrifuge cup through the liquid port 3113 and carbon dioxide of a predetermined concentration can be injected into the centrifuge cup through the gas port 3114 without opening the lid 3.
It should be noted here that the liquid passage 3111 is not limited to only being used for introducing liquid, but may also be used for introducing gas, and similarly, the gas passage 3112 is not limited to only being used for introducing gas, but may also be used for introducing liquid; the liquid passage 3111 and the gas passage 3112 are independent of each other, and when liquid is introduced into one of the liquid passage 3111 and the gas passage 3112, gas can be introduced into the other at the same time, and the two operations of introducing liquid and introducing gas do not interfere with each other.
In one embodiment, the liquid passage 3111 is side-by-side with the gas passage 3112.
In another embodiment, as shown in fig. 7 and 9, the liquid passage 3111 is sleeved in the gas passage 3112, and the second end of the gas passage 3112 is arranged along the rotation axis and is communicated with the inner cavity of the centrifugal cup 1.
In yet another embodiment, as shown in fig. 8, the second end of the gas passage 3112 extends in the radial direction of the centrifuge cup 1 and communicates with the inner cavity of the centrifuge cup 1.
Preferably, as shown in fig. 6, 9 and 10, the cup cover 3 shown in this embodiment is provided with at least one air hole 32, and the air hole 32 is provided with an air permeable membrane.
Specifically, when the centrifuge cup 1 shown in this embodiment is used for cell culture, the gas permeable membrane can prevent external bacteria from entering the centrifuge cup 1, and meanwhile, the gas permeable membrane can realize gas exchange between the inside of the centrifuge cup 1 and the outside, so that the normal respiration of cells in the centrifuge cup 1 is ensured.
It should be noted here that the gas permeable membrane is preferably a hydrophobic gas permeable membrane.
Preferably, as shown in fig. 7, 8, 9 and 10, the cup lid 3 shown in this embodiment includes a top cover 33 and a brim 34, the brim 34 extends along the circumferential direction of the top cover 33, the cup lid 3 covers the rim of the centrifuge cup 1, and the inner side surface of the brim 34 is attached to the outer side wall of the centrifuge cup 1.
Specifically, the cup cover 3 is covered on the cup mouth of the centrifugal cup 1, and the inner side surface of the edge 34 is attached to the outer side wall of the centrifugal cup 1, so that the tightness between the cup cover 3 and the centrifugal cup 1 is ensured.
Preferably, as shown in fig. 4 to 6, when the centrifugal cup 1 is used for culturing cells, in order to facilitate observation of the culture condition of the cells in the centrifugal cup 1, a plurality of observation holes 5 are formed on the centrifugal cup 1 or the cup cover 3, the observation holes 5 are in a normally closed state during the culture of the cells, and when observation is required, the observation holes 5 are opened, and the observation holes 5 are connected with an optical monitoring device for observation.
Preferably, the centrifugation step of the cell centrifugation device shown in the present embodiment is:
step 101, a predetermined volume of separation fluid is added to a centrifuge cup.
Step 102, connecting the centrifuge cup with a rotation driving mechanism, and adding a blood sample into the centrifuge cup.
And 103, starting the rotary driving mechanism, driving the centrifugal cup to rotate by the rotary driving mechanism, so that the liquid pushing plate pushes the blood sample to synchronously rotate along the same rotation direction as the centrifugal cup, and obtaining the cell suspension.
Preferably, in order to compare the influence of the cells on the cell culture results after centrifugation with and without the push plate, the present example performed a control experiment, in which two control groups were provided, and each control group was provided with five experimental samples.
Wherein the first control group is used for centrifuging the cells under the condition that the liquid pushing plate is arranged, and the second control group is used for centrifuging the cells under the condition that the liquid pushing plate is not arranged.
After the centrifugation is finished, the cell yield of each experimental sample in the two control groups is respectively detected, and the detection results are shown in table 1:
table 1 statistics of cell yield after centrifugation:
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Mean value | |
| First control group | 96.1％ | 94.6％ | 93.6％ | 96.4％ | 95.6％ | 95.3％ |
| Second control group | 81.6％ | 76.2％ | 75.4％ | 84.3％ | 80.0％ | 79.5％ |
It can be seen that the cell yield of the first control group is much higher than that of the second control group, and the second control group has a phenomenon that a large number of cells are sheared and damaged in the centrifugation process due to the fact that no liquid pushing plate is arranged, so that the cell yield is reduced.
Further culturing the centrifuged cells, counting the number of the cells in the first control group and the second control group every day, wherein the number of the initially cultured cells is 0.5 hundred million, and the counting result is shown in fig. 11;
statistics at day 14 showed that the number of cells in the first control group proliferated from the initial 0.5 to 1080.0 billion;
statistics at day 7 showed that the number of cells in the second control group proliferated from 0.5 to 200.0 million initially; statistics at day 12 showed that the number of cells in the second control group was 200.5 billion; statistics at day 14 showed that the number of cells in the second control group dropped to 190.5 million.
Therefore, it can be concluded that, although the cell yield of the second control group after the centrifugation was about 83% of the cell yield of the first control group, a large number of cells in the second control group were damaged by shearing to various degrees, and the bioactivity of the cells damaged by shearing was reduced, thereby failing to perform normal culture. Therefore, the liquid pushing plate is arranged in the centrifugal cup, so that shearing damage to cells in the centrifugal process can be effectively reduced, and the cell culture effect is guaranteed.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. A cytocentrifuge device, comprising:
the centrifugal cup is provided with a rotating axis and is used for being connected with the rotating driving mechanism and rotating around the rotating axis under the driving of the rotating driving mechanism so as to be used for centrifuging the blood sample contained in the centrifugal cup;
one or more liquid pushing plates are arranged and arranged in the centrifugal cup; under the condition of the autorotation of the centrifugal cup, the liquid pushing plate is used for pushing the blood sample to synchronously rotate along the same rotation direction as the centrifugal cup.
2. The cytocentrifuge of claim 1,
one end of the liquid pushing plate is close to the rotating axis, and the other end of the liquid pushing plate extends towards the inner side wall of the centrifugal cup;
the projection of the first side edge of the liquid pushing plate on the plane where the bottom of the centrifugal cup is located is linear or arc-shaped, and the second side edge of the liquid pushing plate is perpendicular to the bottom of the centrifugal cup;
and under the condition that the projection of the first side edge of the liquid pushing plate on the plane of the bottom of the centrifuge cup is a straight line, the first side edge of the liquid pushing plate extends along the radial direction of the centrifuge cup.
3. The cytocentrifuge apparatus of claim 2,
a first gap is formed between one end, close to the inner side wall of the centrifugal cup, of the liquid pushing plate and the inner side wall; one end of the liquid pushing plate close to the cup bottom of the centrifugal cup is attached to the cup bottom.
4. The cytocentrifuge apparatus of claim 2,
when the number of the liquid pushing plates is multiple, the liquid pushing plates are uniformly distributed in a circle relative to the rotation axis.
5. The cytocentrifuge of claim 3,
further comprising: a cup cover; the cup cover is matched with the cup opening of the centrifugal cup; a first liquid passing structure is arranged on the cup cover, and a second liquid passing structure is arranged on the centrifugal cup;
the first end of the first liquid passing structure is arranged on the outer side surface of the cup cover and distributed along the rotation axis, and the second end of the first liquid passing structure is arranged on the inner side surface of the cup cover;
the second liquid passing structure comprises a liquid passing pipe and a liquid passing channel; the liquid passing pipe is arranged in the centrifugal cup, the first end of the liquid passing pipe is communicated with the second end of the first liquid passing structure, and the second end of the liquid passing pipe is arranged at the bottom of the centrifugal cup; the liquid passing channel is arranged in the shell wall of the cup bottom, the first end of the liquid passing channel is communicated with the second end of the liquid passing pipe, and the second end of the liquid passing channel is arranged on the inner side surface of the cup bottom and is communicated with the inner cavity of the centrifugal cup.
6. The cytocentrifuge of claim 3,
a third liquid passing structure is arranged in the shell wall of the cup bottom of the centrifugal cup;
the first end of the third liquid passing structure is arranged on the outer side surface of the cup bottom and distributed along the rotation axis, and the second end of the third liquid passing structure is arranged on the inner side surface of the cup bottom and communicated with the inner cavity of the centrifugal cup.
7. The cytocentrifuge apparatus of claim 5,
the first liquid passing structure comprises a connecting pipe;
the connecting pipe and the liquid through pipe are distributed along the rotating axis; a liquid passage and a gas passage are arranged in the connecting pipe, a liquid interface is arranged at the first end of the liquid passage, and the second end of the liquid passage is communicated with the first end of the liquid through pipe; the first end of the gas passage is provided with a gas interface, and the second end of the gas passage is communicated with the inner cavity of the centrifugal cup.
8. The cytocentrifuge apparatus of claim 5,
the cup cover is provided with air holes;
the air holes are at least one, and air permeable films are arranged in the air holes.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121524433.9U CN215465213U (en) | 2021-07-06 | 2021-07-06 | Cell centrifugal device |
| KR1020237045518A KR20240039093A (en) | 2021-07-06 | 2022-06-28 | Cell centrifugation device and cell centrifugation, washing and culture methods |
| PCT/CN2022/101748 WO2023280008A1 (en) | 2021-07-06 | 2022-06-28 | Cell centrifugation apparatus and cell centrifugation, cleaning, and culturing methods |
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| CN202121524433.9U CN215465213U (en) | 2021-07-06 | 2021-07-06 | Cell centrifugal device |
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| CN215465213U true CN215465213U (en) | 2022-01-11 |
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