IT1269180B - Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi - Google Patents

Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi

Info

Publication number
IT1269180B
IT1269180B ITMI940031A ITMI940031A IT1269180B IT 1269180 B IT1269180 B IT 1269180B IT MI940031 A ITMI940031 A IT MI940031A IT MI940031 A ITMI940031 A IT MI940031A IT 1269180 B IT1269180 B IT 1269180B
Authority
IT
Italy
Prior art keywords
production
enzyme
acylase
phalosporanic
carboxybutanamide
Prior art date
Application number
ITMI940031A
Other languages
English (en)
Inventor
Der Goes Wilhelmus Van
Pietro Cesti
Antonella Bernardi
Aldo Bosetti
Giuliana Franzosi
Original Assignee
Mini Ricerca Scient Tecnolog
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mini Ricerca Scient Tecnolog filed Critical Mini Ricerca Scient Tecnolog
Publication of ITMI940031A0 publication Critical patent/ITMI940031A0/it
Priority to ITMI940031A priority Critical patent/IT1269180B/it
Priority to US08/366,457 priority patent/US5612210A/en
Priority to EP95200057A priority patent/EP0663445B1/en
Priority to AT95200057T priority patent/ATE179755T1/de
Priority to DK95200057T priority patent/DK0663445T3/da
Priority to ES95200057T priority patent/ES2132511T3/es
Priority to DE69509417T priority patent/DE69509417T2/de
Priority to JP7019867A priority patent/JP2805594B2/ja
Publication of ITMI940031A1 publication Critical patent/ITMI940031A1/it
Priority to US08/712,389 priority patent/US5801048A/en
Application granted granted Critical
Publication of IT1269180B publication Critical patent/IT1269180B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

L'invenzione si riferisce ad un processo per la produzione di elevate quantità dell'enzima "acido 7?-(4-carbossibutanamido)-cefalosporanico acilasi" (GA) che consiste nella coltivazione, in adatte condizioni colturali, di un ceppo ricombinante di Escherichia coli K12 e nella successiva estrazione dell'enzima dalla coltura ottenuta.La produzione dell'enzima è posta sotto il controllo di un particolare sistema di espressione che può essere indotto dal corn-steep liquor, un componente del terreno di coltura a bassissimo costo.L'enzima può essere impiegato nella preparazione enzimatica dell'acido 7-amminocefalosporanico a partire dall'acido 7?-(4-carbossibutanamido)-cefalosporanico.
ITMI940031A 1994-01-14 1994-01-14 Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi IT1269180B (it)

Priority Applications (9)

Application Number Priority Date Filing Date Title
ITMI940031A IT1269180B (it) 1994-01-14 1994-01-14 Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi
US08/366,457 US5612210A (en) 1994-01-14 1994-12-30 Process for producing 7β-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme
DK95200057T DK0663445T3 (da) 1994-01-14 1995-01-11 Fremgangsmåde til fremstilling af 7beta-(4-carboxybutanamido)-cephalosporansyre-acylase("GA")enzym
AT95200057T ATE179755T1 (de) 1994-01-14 1995-01-11 Herstellungsverfahren des enzyms 7beta-(4-carboxy-butanamido)-cephalosporanische säureacylase
EP95200057A EP0663445B1 (en) 1994-01-14 1995-01-11 Process for producing 7beta-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA) enzyme
ES95200057T ES2132511T3 (es) 1994-01-14 1995-01-11 Procedimiento para la produccion de la enzima acido 7beta-(4-carboxibutanamido)-cefalosporanico-acilasa ("ga").
DE69509417T DE69509417T2 (de) 1994-01-14 1995-01-11 Herstellungsverfahren des Enzyms 7beta-(4-carboxy-butanamido)-cephalosporanischen Säureacylase
JP7019867A JP2805594B2 (ja) 1994-01-14 1995-01-13 ７−（４−カルボキシブタンアミド）−セファロスポラン酸アシラーゼの製法
US08/712,389 US5801048A (en) 1994-01-14 1996-09-11 Process for producing 7B-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI940031A IT1269180B (it) 1994-01-14 1994-01-14 Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi

Publications (3)

Publication Number Publication Date
ITMI940031A0 ITMI940031A0 (it) 1994-01-14
ITMI940031A1 ITMI940031A1 (it) 1995-07-14
IT1269180B true IT1269180B (it) 1997-03-21

Family

ID=11367491

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI940031A IT1269180B (it) 1994-01-14 1994-01-14 Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi

Country Status (8)

Country Link
US (2) US5612210A (it)
EP (1) EP0663445B1 (it)
JP (1) JP2805594B2 (it)
AT (1) ATE179755T1 (it)
DE (1) DE69509417T2 (it)
DK (1) DK0663445T3 (it)
ES (1) ES2132511T3 (it)
IT (1) IT1269180B (it)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
CN1515679A (zh) * 1997-09-09 2004-07-28 ���������ﻯѧ���޹�˾ 不含酯酶的酶

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2464298A1 (fr) * 1979-09-04 1981-03-06 Roquette Freres Procede de trempage du mais, produits ainsi obtenus et leur application a la fabrication de la penicilline
US4440792A (en) * 1982-09-07 1984-04-03 A. E. Staley Manufacturing Company Method for preventing gelation of corn steep liquor
ES2020792A6 (es) * 1990-08-03 1991-09-16 Antibioticos Sa Un procedimiento enzimatico para la presentacion de acido 7-amino-cefa-losporanico y un procedimiento para expresar la enzima 7b (4-carboxibutanamido) cefalosporinacilasa.
DE4136389A1 (de) * 1991-03-18 1992-10-08 Hoechst Ag Verfahren zur herstellung von glutarylacylase in grossen mengen

Also Published As

Publication number Publication date
ATE179755T1 (de) 1999-05-15
DE69509417D1 (de) 1999-06-10
EP0663445B1 (en) 1999-05-06
EP0663445A1 (en) 1995-07-19
ES2132511T3 (es) 1999-08-16
JP2805594B2 (ja) 1998-09-30
US5801048A (en) 1998-09-01
DE69509417T2 (de) 1999-12-16
DK0663445T3 (da) 1999-11-01
JPH07303483A (ja) 1995-11-21
ITMI940031A0 (it) 1994-01-14
US5612210A (en) 1997-03-18
ITMI940031A1 (it) 1995-07-14

Similar Documents

Publication Publication Date Title
HUP0203704A2 (hu) L-Pankolakton hidroláz és eljárás D-pantolakton előállítására
ATE331731T1 (de) Verfahren zur herstellung von diphtheria toxin
DK0625571T3 (da) Mikroorganismer, deres anvendelse samt fremgangsmåde til fremstilling af L-alfa-aminosyrer
PT97397A (pt) Processo para a preparacao de penicilina g acilase e de genes que a codificam
EP1194575A4 (en) MODIFIED HUMAN GRANULOCYTE COLONY, STIMULATING FACTOR, AND METHOD FOR PRODUCING THE SAME
SE7607907L (sv) Enzymkomplex for omvandling av racemiska hydantoiner till optiskt aktiva aminosyror
ATE461997T1 (de) Neuartige glukose-6-phosphat dehydrogenase
ATE334195T1 (de) Proteine mit zellulase-aktivitäten und prozess für ihre herstellung
DE69529953T2 (de) Hitzebeständige rekombinante Enzyme für die Umsetzung von Maltose in Trehalose
EP0859050A4 (en) ALKALINE PROTEASE, PROCESS FOR PRODUCTION AND USE OF SAID PROTEASE, AND MICROORGANISM PRODUCING THE SAME
IT1269180B (it) Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi
IL76649A0 (en) Process for the enzymatic hydrolysis of d-alpha-amino-acid amides
BE902257A (fr) Procede de praparation du d-glucose et de produits d'hydrolyse de l'amidon.
DE3476681D1 (en) Production of secretin
DE58905691D1 (de) Verfahren zur Herstellung von L-alpha-Aminosäuren.
BR0009636B1 (pt) processo para a preparação de insulina madura ou um derivado de insulina madura.
ITMI913375A1 (it) Procedimento per la produzione di neuraminidasi
HUP9802915A2 (hu) Új sztafilokinázszármazékok
ATE346941T1 (de) Protein-disulfidisomerase-gen eines methylotrophen hefestammes
ES2141258T3 (es) Enzima y su uso en la preparacion de acido (s)-pipecolico.
BR9810020A (pt) Processo para a preparação de ampicilina
DE730028T1 (de) Neuartige Cholesterinoxidase
IT1245385B (it) Procedimento migliorato per la preparazione di polipeptidi maturi
JP2838005B2 (ja) 新規なγ−ポリグルタミン酸分解酵素
DE2922496A1 (de) Verfahren zur spezifischen abspaltung von proteinsequenzen aus proteinen

Legal Events

Date Code Title Description
0001 Granted